193 results on '"PZR"'
Search Results
2. RNA-Seq Analysis Reveals Altered Expression of Cell Adhesion-Related Genes Following PZR Knockout in Lung Cancer Cells.
- Author
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Fu, Ying, Li, Guodong, Fu, Xueqi, Xing, Shu, and Zhao, Zhizhuang Joe
- Abstract
Protein zero related (PZR) serves as a substrate and anchor protein for SHP-2, the product of the proto-oncogene PTPN11 that is frequently mutated in cancers. The expression level of PZR is elevated in various cancers, which is correlated with an unfavorable prognosis. The role of PZR in lung cancer is not fully studied. To investigate how PZR affects signaling pathways involved in LUAD development, we utilized the CRISPR technology to knock out PZR expression in SPC-A1 lung adenocarcinoma cells and then conducted RNA sequencing to profile the transcriptome. Our results showed that 226 genes exhibited differential expressions in PZR-knockout SPC-A1 cells vs wild-type cells. Many of the genes encode proteins involved in cell adhesion, migration, actin cytoskeleton organization, and regulation of cell shape. Furthermore, our experimental data showed that PZR-knockout SPC-A1 cells displayed faster attachment to tissue culture dishes and slower detachment from the dishes upon EDTA treatment. The data suggest an important role of PZR in cell–matrix interaction and may provide new insights into the signaling events that regulate cancer development. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Tüketilen Bazı Probiyotik Preparatların İçerdiği Bakteri Seviyelerinin Belirlenerek PZR ile Doğrulanması
- Author
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Özen Yurdakul, Elif Gizem Yılmaz, Erdi Şen, and Soner Tutun
- Subjects
bifidobacterium spp. ,lactobacillus spp. ,probiyotik mikroorganizmalar ,probiyotik-prebiyotik preparatlar ,pzr ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
Bu çalışma, takviye olarak kullanılan probiyotik preparatların etikette belirtildiği gibi içerdiği bakterilerin canlılık ve seviyelerinin incelenmesi amacıyla yapılmıştır. Bu amaçla farklımarkalara ait Lactobacillus ve Bifidobacterium türlerini içeren toplam 15 farklı preparat temin edildi. Örnekler Lactobacillus ve Bifidobacterium türlerini yönünden mikrobiyolojik ekime tabii tutuldu. Yapılan mikrobiyolojik analiz sonucunda örneklerin 13’ünde (%87) canlı bakteri saptanırken 2 örnekte (%13) üreme olmadığı gözlemlendi. Lactobacillus spp. içeren probiyotik preparatlarda bakteri sayısı ortalama 5,9×1010 kob/g; Bifidobacterium spp. içeren probiyotik preparatlarda ise bakteri sayısının ortalama 1,3×109 kob/g olduğu görüldü. Gram boyama, katalaz testivekarbonhidrat fermentasyon testi yapılan suşların Polimeraz Zincir Reaksiyonu (PZR) analiziile doğrulamaları yapıldı. PZR analizi sonucunda, 13 örnekte Lactobacillus ve Bifidobacterium türleri tespit edildi. Sonuç olarak tüketime sunulan hazır probiyotik preparatlarda belirtilen suşların varlığı, canlılığı ve miktarı oldukça önemlidir. Etikette yazılan bilgilerin doğruluk payının %87 olduğu yaptığımız çalışmamızda görüldü.
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- 2024
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4. Detection and Molecular Characterization of Canine Coronavirus Based on Partial Membrane Gene Sequences.
- Author
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KARAPINAR, Zeynep and TİMURKAN, M. Özkan
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CORONAVIRUSES , *FOOD contamination , *MOLECULAR epidemiology , *MEMBRANE proteins , *INFECTION control - Abstract
Canine coronavirus (CCoV) infection in dogs is common all over the world and progresses with gastroenteritis findings. Infection as a result of complications with secondary factors may result in death, especially in puppies. The virus, which is excreted in the feces, spreads indirectly through the contamination of food, water, and the environment. This study, it was aimed at revealing the CCoV infection and obtaining current molecular information about the infection. In addition, molecular characterization of CCoV strains circulating in the region was made based on the M (membrane protein) gene. The study material consisted of stool samples from 12 dogs with gastroenteritis findings. The amplified PCR products were subjected to sequence analysis and a phylogenetic tree was constructed by comparing them with different reference CCoV isolates from GenBank. In the phylogenetic tree, 1 of the 5 positive samples was determined to be CCoV-I, and 4 samples were determined to be CCoV-IIa. It was determined that the strains obtained were 85.4 - 97.7% similar among themselves and 82.7-98% similar to other strains obtained from GenBank. As a result of study, current molecular information about CCoV circulating in the Balıkesir region was obtained. With this study, it is thought that new research on the existence and molecular epidemiology of CCoV infection in Türkiye will make important contributions to vaccine studies and the control of infection. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Investigation of the Presence and Prevalence of Listeriosis in Clinical Samples in Van and its Region.
- Author
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AKAR, Kadir, SANİOĞLU GÖLEN, Gökçenur, BABAOĞLU, Ali Rıza, and EKİN, İsmail Hakkı
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LISTERIOSIS ,SILAGE ,OLIGONUCLEOTIDES ,POLYMERASE chain reaction ,CLINICAL trials - Abstract
Copyright of Harran University Journal of the Faculty of Veterinary Medicine / Harran Üniversitesi Veteriner Fakültesi Dergisi is the property of Harran University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
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6. InDel Variations of PRL and GHR Genes Associated with Litter Size in Pirlak sheep breed.
- Author
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ATAY, Sude, YURDAGUL, Kadriye Gul, BILGINER, Umit, KARSLI, Taki, and DEMIR, Eymen
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SHEEP breeds , *SHEEP breeding , *SOMATOTROPIN receptors , *GENETIC variation , *GENES , *GENE expression profiling - Abstract
Numerous molecular genotyping methods are available to analyse local livestock populations at molecular level in which traditional Polymerase Chain Reaction (PCR) guided by specific oligonucleotides is a fast and costeffective method to investigate single genes. Until today, many genes which are of major effects on litter size have been reported in sheep. Genetic variations in these genes shaping the expression profile at DNA level may lead to differences in litter size among the sheep breeds. This is the first attempt to investigate insertion/deletion (inDel) variations in Prolactin (PRL) intron 2 and Growth Hormone Receptor (GHR) intron 3 and intron 4 genes in Pirlak sheep breed via traditional PCR technique. A total of 100 unrelated animals sampled from representative herds reared in Antalya were genotyped based on absence/presence of 23 base pairs (bp) length inDel in which three genotypes (II, ID, and DD) were detected in all loci. I and D allele frequency were 0.421 and 0.579, respectively in terms of PRL-intron 2 locus. I / D allele frequencies were found as 0.599 / 0.401 and 0.372 / 0.628 in GHR intron 3 and intron 4, respectively. The lowest II (0.181) and DD (0.177) genotype frequencies were detected in GHR-intron 4 and GHR-intron 3 loci, respectively. The lowest (0.177 for DD) and highest (0.448 for ID) genotype frequencies were detected in GHR intron 3 locus across the population. Significant deviation from Hardy- Weinberg Equilibrium (HWE) was detected only in PRL-intron 2 locus. The results of the present study confirm that Pirlak breed conserves sufficient genetic variation in PRL and GHR gene regions which could be utilized in selection strategies in order to increase litter size in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
7. Postmortem Süreçte COVID-19 Enfeksiyon Etkeninin Pozitif Kalma Süresi.
- Author
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Göçeoğlu, Ümit Ünüvar, Balcı, Yasemin, Togan, Turhan, Aksözek, Alper, Gürsoy, Canan, Çitil, Burak Ekrem, Çatal, Yeşim Gök, and Ekinci, Ecesu
- Abstract
Copyright of Bulletin of Legal Medicine / Adli Tıp Bülteni is the property of Galenos Yayinevi Tic. LTD. STI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
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8. Multietiological abortion due to Brucella melitensis and Chlamydia abortus in a sheep fetus.
- Author
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Deniz, İbrahim and Oruç, Ertan
- Subjects
BRUCELLA melitensis ,CHLAMYDIA ,IMMUNOHISTOCHEMISTRY ,BRONCHOPNEUMONIA ,HISTOPATHOLOGY - Abstract
Copyright of Etlik Veteriner Mikrobiyoloji Dergisi is the property of Veteriner Kontrol Merkez Arastirma Enstitusu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
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9. Determination of Trichomonas vaginalis Frequency Among Symptomatic Cases in Muğla Sıtkı Koçman Hospital Using Different Methods (Direct Microscopy, Culture, PCR and Immunochromatographic Method).
- Author
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Sankur, Funda, Ertuğ, Sema, Malatyalı, Erdoğan, Tileklioğlu, Evren, Yıldız, İbrahim, and Ertabaklar, Hatice
- Subjects
TRICHOMONAS vaginalis ,MICROSCOPY ,POLYMERASE chain reaction ,DEMOGRAPHIC surveys ,OBSTETRICS - Abstract
Copyright of Meandros Medical & Dental Journal is the property of Galenos Yayinevi Tic. LTD. STI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
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10. Evaluation of Toxoplasma gondii Molecular Test Results in Patients Admitted to Ankara City Hospital: Three-Year Retrospective Analysis
- Author
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Fisun Kırca and Filiz Demirel
- Subjects
toxoplasma gondii ,toksoplazmoz ,pzr ,hiv. ,toxoplasmosis ,pcr ,hiv ,Medicine - Abstract
Aim: Toxoplasma gondii infects about 25-30% of the world population. Toxoplasmosis is generally asymptomatic in immunocompetent individuals, but the infection can be life threatening in congenitally infected children and immunocompromised individuals. In this study, it is aimed to analyse the molecular test results of patients suspected with toxoplasmosis, retrospectively. Material and Method: A total of 647 clinical samples investigated for T. gondii DNA with real-time PCR during the three-year period between 2019 and 2022 were evaluated retrospectively. For the qualitative detection of T. gondii, DNA isolation and DNA amplification were performed using commercial DNA extraction kit (Qiagen, Germany) and real time PCR kit (Sacace Biotechnologies, Italy), respectively. The data on the demographic and clinical parameters of the patients were obtained from the laboratory information management system. Results: Out of 647 patients investigated for T. gondii DNA with real-time PCR, 51.8% were female and the mean age of the patients was 37.03 years. Among all patients, five were positive for T. gondii DNA with real-time PCR and the frequency of a positive PCR result was found 0.8% of all samples analysed. The most frequently positive clinical sample was blood (80%). Among five patients with T. gondii DNA positivity, one was diagnosed with congenital toxoplasmosis, four were HIV-infection. Conclusion: Fast and accurate diagnosis of toxoplasmosis especially in immunosuppressed patients is crucial for rapid and specific treatment. Further studies are needed to understand the importance of molecular tests, in addition to the serological tests, in the diagnosis of toxoplasmosis.
- Published
- 2022
- Full Text
- View/download PDF
11. STREPTOCOCCUS MILLERI GRUP (SMG) BAKTERİLERİNİN BİYOKİMYASAL VE MOLEKÜLER YÖNTEMLERLE TANIMLANMASI.
- Author
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GÜNEL, Gülşen and GÜRLER, Bülent
- Subjects
ANTIBIOTICS ,BIOCHEMISTRY ,BIOMARKERS ,ERYTHROMYCIN ,PHENOMENOLOGICAL biology ,STREPTOCOCCAL diseases ,ANTI-infective agents ,MOLECULAR biology ,BACTERIOPHAGE typing ,DISEASE susceptibility ,CHLORAMPHENICOL ,RESEARCH funding ,POLYMERASE chain reaction - Abstract
Copyright of Journal of Advanced Research in Health Sciences (JARHS) / Sağlık Bilimlerinde İleri Araştırmalar Dergisi (SABİAD) is the property of Journal of Advanced Research in Health Sciences (JARHS) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
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12. Mikrobiyolojide En Yaygın Moleküler Tanı Yöntemi: Polimeraz Zincir Reaksiyonu.
- Author
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TÜREDİ, Oğuz Kağan and ŞEKER, Esra
- Abstract
Copyright of Harran University Journal of the Faculty of Veterinary Medicine / Harran Üniversitesi Veteriner Fakültesi Dergisi is the property of Harran University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
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13. Koyun ve Keçi Atık Fetüslerinde Toxoplasma gondii'nin Prevelansının PZR ile Taranması.
- Author
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AKPINAR, Rahşan, KIZILTEPE, Şemistan, KAYA, Selma, AYDIN, Coşkun, and TÜRLEK, Şakir Önder
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TOXOPLASMA gondii ,POLYMERASE chain reaction ,PARASITIC protozoa ,DOMESTIC animals ,SHEEP farming ,SHEEP ranches - Abstract
Copyright of Firat Universitesi Saglik Bilimleri Veteriner Dergisi is the property of Firat Universitesiu, Saglik Bilimleri Enstitusu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
14. PZR with the help of markers in the cotton plant DNA
- Author
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Mullayev, Dilshod Akhmatovich and Kamilova, Mohinabonu Bobirqizi
- Published
- 2022
- Full Text
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15. Akut Gastroenteritli Hastaların Klinik Örneklerinde Salmonella, Shigella ve Campylobacter Türlerinin Kültür Yöntemi ve Moleküler Yöntem ile Tespit Edilmesi.
- Author
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Avcı, Gamze, Kiraz, Nuri, Duran, Hülya, and Erdal, Berna
- Abstract
Copyright of Osmangazi Journal of Medicine / Osmangazi Tip Dergisi is the property of Eskisehir Osmangazi University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
- Full Text
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16. Evaluation of Echinococcus DNA by polymerase chain reaction (PCR) in cystic Echinococcosis of the liver.
- Author
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Kırış, Mehmet Tolga, Ergün, Sefa, Akıncı, Ozan, Ergin, Sevgi, Velidedeoğlu, Mehmet, Kocazeybek, Bekir Sami, and Göksoy, Ertuğrul
- Subjects
- *
DNA polymerases , *POLYMERASE chain reaction , *ECHINOCOCCUS , *ECHINOCOCCOSIS , *RAPD technique , *EDWARDSIELLA tarda - Published
- 2022
- Full Text
- View/download PDF
17. First Detection of Giardia duodenalis in Cats in Mardin Province.
- Author
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ASLAN ÇELİK, Burçak
- Subjects
CATS ,GIARDIA ,GIARDIASIS ,PROVINCES ,PROTOZOA - Abstract
Copyright of Firat Universitesi Saglik Bilimleri Veteriner Dergisi is the property of Firat Universitesiu, Saglik Bilimleri Enstitusu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
18. Benötigt subgingivale Instrumentierung vorherige professionelle Zahnreinigung?
- Author
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Jentsch, Holger
- Subjects
ORAL hygiene ,PROFESSIONAL relationships ,PROFESSIONAL employees ,MOTIVATION (Psychology) - Abstract
Copyright of Parodontologie: die Zeitschrift für die Praxis (Berlin, Germany) is the property of Quintessenz Verlags GmbH and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
19. Toxoplasma gondii 529 baz çifti büyüklüğünde tekrar bölgesine (RE) özgü hızlı döngü aracılı izotermal amplifikasyon testinin geliştirilmesi ve analitik hassasiyetinin belirlenmesi
- Author
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Karakavuk, Muhammet, Can, Hüseyin, Karakavuk, Tuğba, Gül, Ceren, Alak, Sedef Erkunt, Gül, Aytül, Döşkaya, Aysu Değirmenci, Ün, Cemal, Gürüz, Adnan Yüksel, and Döşkaya, Mert
- Abstract
Copyright of Ege Journal of Medicine is the property of Ege University, Faculty of Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
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20. FIRST MOLECULAR DETECTION AND PHYLOGENETIC ANALYSIS OF ANAPLASMA PHAGOCYTOPHILUM IN HORSES IN MUŞ PROVINCE OF TURKEY.
- Author
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Oğuz, Bekir
- Subjects
ANAPLASMA phagocytophilum ,HORSES ,THROMBOCYTOPENIA ,PHYLOGENY - Abstract
Copyright of Journal of Health Sciences of Kocaeli University / Kocaeli Üniversitesi Sağlık Bilimleri Dergisi is the property of Institute of Health Sciences of Kocaeli University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
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21. Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey.
- Author
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OĞUZ, Bekir, DEĞER, M. Serdar, and KOŞAL, Sena
- Subjects
- *
RIBOSOMAL RNA , *SARCOCYSTIS , *RIBOSOMAL DNA , *TRYPANOSOMA cruzi , *WATER buffalo , *BOS , *MICROSCOPY - Abstract
This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from İpekyolu, Tuşba, Edremit, Erciş, and Gevaş districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic Local Alignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
22. PZR suppresses innate immune response to RNA viral infection by inhibiting MAVS activation in interferon signaling mediated by RIG-I and MDA5.
- Author
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Deng, Rilin, Zhang, Lini, Chen, Shengwen, Li, Xinran, Xue, Binbin, Li, Huiyi, Xu, Yan, Tian, Renyun, Liu, Qian, Wang, Luoling, Liu, Shun, Yang, Di, Li, Penghui, Tang, Songqing, and Zhu, Haizhen
- Subjects
- *
VIRUS diseases , *IMMUNE response , *RNA , *PHOSPHOPROTEIN phosphatases , *CUCUMBER mosaic virus , *ANTIVIRAL agents , *INTERFERONS , *PROTEIN-tyrosine phosphatase - Abstract
RNA viral infections seriously endanger human health. Src homology 2 (SH2) domain-containing protein tyrosine phosphatase 2 (SHP2) suppresses innate immunity against influenza A virus, and pharmacological inhibition of SHP2 provokes hepatic innate immunity. SHP2 binds and catalyzes tyrosyl dephosphorylation of protein zero-related (PZR), but the regulatory effect of PZR on innate immune response to viral infection is unclear. In this study, the transcription and protein level of PZR in host cells were found to be decreased with RNA viral infection, and high level of PZR was uncovered to inhibit interferon (IFN) signaling mediated by RIG-I and MDA5. Through localizing in mitochondria, PZR targeted and interacted with MAVS (also known as IPS-1/VISA/Cardif), suppressing the aggregation and activation of MAVS. Specifically, Y263 residue in ITIM is critical for PZR to exert immunosuppression under RNA viral infection. Moreover, the recruited SHP2 by PZR that modified with tyrosine phosphorylation under RNA viral infection might inhibit phosphorylation activation of MAVS. In conclusion, PZR and SHP2 suppress innate immune response to RNA viral infection through inhibiting MAVS activation. This study reveals the regulatory mechanism of PZR-SHP2-MAVS signal axis on IFN signaling mediated by RIG-I and MDA5, which may provide new sight for developing antiviral drugs. • RNA viral infection decreases PZR level in host cells. • Through localizing in mitochondria, PZR inhibits MAVS aggregation, suppressing IFN signaling mediated by RIG-I and MDA5. • TM and Y263 residue in ITIM of PZR are critical for exerting immunosuppression to RNA viral infection. • SHP2 may inhibit phosphorylation activation of MAVS under RNA viral infection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
23. Microbiological Diagnosis of COVID-19.
- Author
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SÜMBÜL, Bilge and DOYMAZ, Mehmet Ziya
- Subjects
- *
COVID-19 testing , *MEDICAL personnel , *PERSONAL protective equipment , *COVID-19 , *SARS-CoV-2 - Abstract
Various tests are used to detect the severe acute respiratory syndrome-coronirus-2 (SARS-CoV-2) virus causing Coronavirus disease-19 (COVID-19) disease. Today, the realtime (RT) -PCR test combined with the reverse-transcription reaction is the gold standard method used to diagnose SARS-CoV-2. This method is referred to as quantitative realtime PCR (RT-qPCR) because it determines not only the presence of SARS-CoV-2 but also the amount of virus in the specimen. Due to the use of virus-specific primers, the specificity of the tests is considered to be 100%. For this test, swab samples taken from the upper respiratory tract such as nasopharyngeal and throat, samples from the lower respiratory tract areas such as sputum and bronchoalveolar lavage fluid, rectal swab, feces, serum and urine samples are preferred. Correct use of personal protective equipment (PPE) by healthcare professionals during sampling and testing is important. Rapid antigen tests used in addition to RT-qPCR test for the diagnosis of SARS-CoV-2 are advantageous due to the theoretical rapid result time and low cost, but the sensitivity of this method is known to be very low. Virus detection in cell cultures can be used to detect SARS-CoV-2, but it is not for routine diagnostic because the results take a long time, require labor, and expertise. Serological tests are frequently used in the diagnosis and follow-up of this disease. These are mainly ELISA, CLIA, immunofluorescence test (IFA), western blot (WB), protein microarray (microarray) and neutralization. ELISA based immunoglobulin (Ig)M and IgG antibody tests have more than 95% specificity in the diagnosis of COVID-19. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
24. Klinik Pseudomonas aeruginosa İzolatlarında Siprofloksasin Direnci ve Direnç Mekanizmalarının Araştırılması.
- Author
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Akel, Nilüfer Uzunbayır, Tekintaş, Yamaç, Yılmaz, Fethiye Ferda, Öztürk, İsmail, Ökeer, Mustafa, Aydemir, Sabire Şöhret, Çilli, Fatma Feriha, and Hoşgör-Limoncu, Mine
- Abstract
Copyright of ANKEM Antibiyotik & Kemoterapi Dergisi is the property of ANKEM Antibiyotik & Kemoterapi Dergisi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
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25. The Molecular Identification and Antibiotic Sensitivity of Streptococcus equi Strains Isolated from Horses.
- Author
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ÇALIŞKAN, Abdullah Burak and TEL, Osman Yaşar
- Abstract
Copyright of Harran University Journal of the Faculty of Veterinary Medicine / Harran Üniversitesi Veteriner Fakültesi Dergisi is the property of Harran University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
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26. Clinical Evaluation of Cases of Pandemic H1N1 Influenza in Sanliurfa Province in Epidemic Period
- Author
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Halil Kazanasmaz, Süleyman Geter, Abdullah Solmaz, Şakir Genç, and Hüseyin Gümüş
- Subjects
h1n1 ,influenza ,oseltamivir ,pzr ,pnömoni ,pcr ,pneumonia ,Medicine - Abstract
Aim: In this study, we aimed to share our experience with 75 patients admitted to the hospital who adhered to the definition of influenza-like illness.Patients and Methods: Pandemic 2009 influenza positive and negative cases with RNA (ribonucleic acid) detected by PCR (polymerase chain reaction) method were compared in terms of age, gender and underlying disease relationships, laboratory and radiological imaging results, hospital stay and intensive care unit stay and discharge patterns. Results: There was no significant difference in sex distribution, age and chest x-ray findings between the two groups. When the CRP(C-reactive protein) and platelet levels of the groups were compared between the groups, there was a meaningful difference between them, but there was no significant difference in terms of white cell, hemoglobin, neutrophil and lymphocyte counts. The mean CRP level in the H1N1 positive group was significantly higher than the H1N1 negative group. In the H1N1 positive group, the mean platelet amount was significantly lower than in the H1N1 negative group. Conclusion: Clinical follow-up of pandemic H1N1 influenza cases is very important. Pandemic influenza (H1N1) was thought to be fatal in some cases in the underlying chronic illness.
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- 2018
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27. The Role of Interleukin-4 VNTR Polymorphism in Dysmenorrhea Development.
- Author
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Esen, Mehmet, Nursal, Ayşe Feyda, Duman, Esra, and Yiğit, Serbülent
- Subjects
- *
ACADEMIC medical centers , *ALLELES , *DYSMENORRHEA , *GENETIC polymorphisms , *INTERLEUKINS , *LONGITUDINAL method , *POLYMERASE chain reaction , *CASE-control method , *DATA analysis software , *DESCRIPTIVE statistics , *SEQUENCE analysis , *GENOTYPES , *DISEASE risk factors - Abstract
Aim: Primary dysmenorrhea (PD) is among the most common gynecological diseases in young women presenting to emergency department. It has been shown that cytokines played roles in PD pathogenesis. Interleukin-4 (IL-4), a cytokine, regulates multiple biological functions. The objective of the present study was to examine possible relationship between IL-4 variable number of tandem repeat (VNTR) polymorphism and susceptibility to PD. Methods: This study was based on a prospective cohort study design. A total of 120 patients with PD and 116 healthy controls, who presented to the emergency department between 01.12.2018 and 01.12.2019, were included in the study. IL-4 VNTR was genotyped by polymerase chain reaction (PCR). The results of the analyses were evaluated in terms of statistically significant differences. Results: The prevalence of genotypes of P1/P1, P1/P2, and P2/ P2 for IL-4 VNTR were 1.72%, 34.4%, and 63.7% in patients with PD, and 0.8%, 26.6%, and 72.5% in controls, respectively. There was no significant difference in distribution of genotypes and allele frequencies of IL-4 VNTR between the groups (p>0.05). Conclusion: This research is the first study to examine the relationship between IL-4 VNTR and PD. The data of the present study did not support a relationship between IL-4 VNTR and PD risk. [ABSTRACT FROM AUTHOR]
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- 2020
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28. ACAN Gene VNTR Polymorphism and Intervertebral Disc Degeneration in a Turkish Population.
- Author
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Öz, Tuba, Kaya, İsmail, Nursal, Ayşe Feyda, Aydın, Hasan Emre, Demir, Osman, and Yiğit, Serbülent
- Subjects
- *
GENETICS of disease susceptibility , *ALLELES , *GENETIC polymorphisms , *GLYCOPROTEINS , *INTERVERTEBRAL disk , *SPINE diseases , *POLYMERASE chain reaction , *SEX distribution , *CASE-control method , *DESCRIPTIVE statistics - Abstract
Aim: Intervertebral disc degeneration (IVDD) is caused by several genetic and environmental factors. Aggrecan is the major component of intervertebral disk matrix proteoglycan with multiple functional domains. The aim of this study was to investigate the possible association between ACAN (coding aggrecan) gene variable number tandem repeat (VNTR) polymorphism and susceptibility to IVDD. Methods: Two hundred and sixty subjects participated in this study. The disease group comprised 150 patients diagnosed with symptomatic IVDD. The control group consisted of 110 healthy subjects. The ACAN gene VNTR region was analyzed using the polymerase chain reaction (PCR) method. Results: The most common allele in the patient and the control group was 27 repeat allele (49% and 34.55%, respectively). Allele 26 was more frequent in males compared to females (p=0.030). Allele 21 and 23 were more common in ones living in rural areas (p=0.030) while allele 27 was the most frequent in ones living in urban areas (p<0.001). Allele 26, allele 29 and allele 30 were less frequent in the patient group than in the control group (p=0.013, p=0.001 and p=0.001, respectively) while allele 27 was more common in the patient group compared to the control group (p=0.001). Conclusion: Our results showed that ACAN VNTR allele 27 had a positive relationship with IVDD susceptibility in a Turkish population. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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29. Düzce COVID 19 PZR Testleri Nerede, Nasıl, Hangi Koşullarda Çalışılıyor?
- Author
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Öztürk, C. Elif, Erdoğan, Ziya, Atik, Dursun, Esen, Taner, Dönmez, Betül, Memiş, Nagihan, BanuHümeyraKeskin, Kayabaşı, Eda, Kaya, Sare, Köse, Ezgi, Kahraman, Gözde, Duran, Pelin, Çalışkan, Emel, Öksüz, Şükrü, and Şahin, İdris
- Subjects
- *
CLINICAL pathology , *ENZYME-linked immunosorbent assay , *EPIDEMICS , *HOSPITALS , *POLYMERASE chain reaction - Abstract
Shortly after the first cases were reported in late 2019 in Wuhan Province of China, SARS CoV 2 spread all over the world and was declared a pandemic by the World Health Organization on March 12, 2020. In Turkey the first case was in March 10, 2020 and March 25, 2020 in Düzce. Duzce University Faculty of Medicine, Department of Medical Microbiology, PCR laboratory was declared to be "T.C. Ministry of Health Düzce authorized Covid 19 PCR laboratory " in 28.03.2020. Samples are sent to our laboratory from all provincial and district hospitals, private hospitals and public health institutions in Düzce. This is the only Covid 19 center in our city and it works for 7 days a week. In our laboratory, Covid 19 antibody tests are carried out with card tests and ELISA methods. A total of 8500 COVID 19 PCR tests and 2200 anti Covid19 ELISA total antibody tests were studied in our laboratory within three months. Although covid 19 patients are not followed in our hospital, pandemic process continues in our laboratory. In addition, all PCR studies and IFA studies of the hospital are carried out in this laboratory. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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30. Pandemi Sürecinde Düzce Üniversitesi Hastanesi: Başhekimlik Yönünden.
- Author
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Akcan, Fatih Alper, Öneç, Kürşad, Annakkaya, Ali Nihat, Pehlivan, Mevlüt, Karaduman, Zekeriya Okan, Balbay, Öner Abidin, Çakar, Nigar Demircan, Eravcı, Fatma, Çelik, Muhammet, Kaplan, Zekeriya, Şen, Nevin, Ergün, Hacer Ak, Kuzyaka, İlknur, Bulut, Feryal, Yalçın, Bedri, Öner, Seçkin, Odabaşı, Nuray, Köklü, Şaban, Koku, Sercan, and Şengül, Kadriye
- Subjects
- *
ACADEMIC medical centers , *EMERGENCY management , *EMOTIONS - Abstract
We aimed to compile the effects of the preparations on the new coronavirus outbreak (Covid-19) in the largest pandemic, which is still visible in the world. Pandemics have caused millions of people to become ill, disabled and death for centuries. Thanks to past pandemics that deeply affect the society health, socially and economically, preparations have been made again against possible pandemics. Preparations may not go as planned. Desperation may occur for unforeseen situations. Despite these despair, the tool that can contribute in practice besides the basic principles in combating pandemics can be obtained from the past. Like every pandemic, the pandemic will end one day. Those applied and not implemented after the outbreak will be evaluated and will remain a lesson from the past for future outbreaks. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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31. Şişelenmiş içme sularında Helicobacter pylori DNA'sının araştırılması: Bir ön çalışma.
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KALAYCI-YÜKSEK, Fatma
- Subjects
- *
GASTRIC mucosa , *MUCOSA-associated lymphoid tissue lymphoma , *BOTTLED water , *BACTERIAL DNA , *WATER pollution , *DRINKING water , *FECAL contamination , *WATER filters - Abstract
Objective: Helicobacter pylori is a bacterium which colonizes the human gastric mucosa and known to affect half of the world's population. H. pylori is defined as the aetiological agent of peptic ulcer, gastric adenocarcinoma and mucosa-associated lymphoid tissue lymphoma. However, the natural host and reservoir have not been clearly identified. The ability of adaptation and biofilm formation, which play an important role in the virulence of the bacterium, constituted the basis of studies about water contamination. In relation with this, bacterial DNA was detected in drinking water, surface water, groundwater and wastewater in many studies. It has been suggested that the transmission of bacterium occurs via fecal-oral, oraloral and gastro-oral routes, additionally contaminated foods and water may be source of infection. In the present preliminary study, it was aimed to investigate the presence of H. pylori DNA in drinking water samples. Methods: Thirty five different trademarked drinking water samples (each one 500 mL) were collected from March-June 2019 in all around Istanbul. It was observed that water samples have a slightly alkaline pH ranging from 6.6 to 8.45. All water samples were filtrated using 0.22 µM filter membranes which were incubated on Brain Heart Infusion (BHI) broth for half hour at room temperature and filter included broths were centrifuged. Then bacterial DNA was extracted. Polymerase Chain Reaction (PCR) was used for the detection of the ureC (glmM) gene. The PCR products were visualised in %1.5 agarose gel electrophoresis. Results: In this study H. pylori DNA was not detected in any of the water samples tested. Conclusion: This preliminary study results have suggested that, drinking waters do not have a role in transmission of H. pylori in this geographical area. However, it is important to carry out more extensive studies since the number of samples examined in this study was limited and only bottles sold in 500 mLpackaging are included. Therefore it may be concluded that different water sources such as 19L flagon bottled drinking waters, tap waters and water samples from various tanks may be useful for clarifying the role of water for transmission route of this pathogen. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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32. First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan.
- Author
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ALTAY, Kursat, AYDIN, Mehmet Fatih, AYTMIRZAKIZI, Ayperi, JUMAKANOVA, Zarima, CUNUSOVA, Ayday, and DUMANLI, Nazir
- Subjects
- *
MYCOPLASMA , *CANDIDATUS , *MAXIMUM likelihood statistics , *DOGS - Abstract
Mycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp) have been investigated using species specific PCR and sequencing in 170 dogs from Kyrgyzstan. Maximum likelihood estimation (MLE) of the infection rates with 95% confidence intervals (CI) was calculated. The molecular prevalence of hemoplasma infection was 5.29% (CI 2.57-9.34). It was found that, five (2.94%, CI 1.06-6.22) samples were found to be infected with Mhc, one (0.59%, CI 0.03-2.57) sample with CMhp and three (1.76%, CI 0.44-4.52) samples with both species. These results demonstrate that dogs can be exposed to each haemoplasma species and provide first molecular evidence for these species in Kyrgyzstan. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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33. Yerfıstığından İzole Edilen Aspergillus section Flavi Türlerinin Tanımlanması ve Mikotoksijenik Özelliklerinin Belirlenmesi.
- Author
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Lavkor, Işılay
- Subjects
- *
THIN layer chromatography , *HIGH performance liquid chromatography , *POLYMERASE chain reaction , *AFLATOXINS , *TUBULINS , *MYCOTOXINS , *PEANUTS , *DNA - Abstract
This study is aimed to identify 50 A. section Flavi isolates from peanut samples collected in the Adana and Osmaniye provinces of Turkey by using morphological and polymerase chain reaction (PCR), and to determine the in vitro aflatoxin and cyclopyazonic acid formation properties of the isolates. DNA was extracted for the identification of Aspergillus spp., and ß-tubulin gene region on DNA was amplified by PCR. Identification of species was determined by comparing the result of sequences which belong to partial ß-tubulin of the sequences with known ribosomal sequences using Basic Local Alignment Search Tool (BLAST). In the study, out of a total 50 fungal isolates, 17 were identified as A. parasiticus, and 33 were identified as A. flavus. As a result of Blast analysis of ß-tubulin gene sequence comparisons A. flavus and A. parasiticus showed a major similarity in ranges 89-100% and 96-100%, respectively. Furthermore, all of the A. flavus and A. parasiticus isolates were determined as aflatoxin producers in the range from 0.45 to 4521.64 µg/L and 0.22 to 2562.87 µg/L with high-performance liquid chromatography (HPLC) under in vitro conditions, respectively. Total 32 A. flavus isolates produced cyclopiazonic acid in the range between 0.35-8.21 µg/g with thin layer chromatography (TLC). The present study provides a new method on molecular characterization of A. section Flavi on peanut samples collected from the Adana and Osmaniye provinces of Turkey. This research also demonstrated that A. flavus and A. parasiticus were responsible for mycotoxin contamination on peanut samples collected from Adana and Osmaniye provinces. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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34. Auf das klinische Protokoll kommt es an - PZR, UPT und GBT.
- Author
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Bastendorf, Klaus-Dieter and Strafela-Bastendorf, Nadine
- Abstract
Copyright of Quintessenz Zahnmedizin is the property of Quintessenz Verlags GmbH and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
35. A new method to quantify atmospheric Poaceae pollen DNA based on the trnT-F cpDNA region.
- Author
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Alan, Şenol, Sarışahin, Tuğba, Şahin, Aydan Acar, Kaplan, Ayşe, Erdoğan, İbrahim, and Pınar, Nur Munevver
- Subjects
- *
DNA primers , *CHLOROPLAST DNA , *POLLEN , *DNA , *DNA fingerprinting , *GRASSES , *POLYMERASE chain reaction - Abstract
Background: Pollen, mold spores, bacteria and viruses are the main biological substances in the atmosphere causing allergic symptoms and disease. Distinguishing pollen and spores is quite time consuming and requires a trained expert. There is a different approach to identification of these substances such as microscopic analysis. However, DNA based identification of these is becoming popular recently. Objective: We evaluated the correlation between the quantity of DNA, which was amplified using trnT-F cpDNA specific primers in samples obtained from a high volume air sampler (HVAS), and concentration of Poaceae pollen collected with a Burkard trap. Materials and methods: Here, we present a method for identifying and quantifying airborne Poaceae pollen using a single step polymerase chain reaction (PCR) technique. Forty daily air samples were collected by HVAS. The method was optimised using two different methods (M1 and M2) and the trnT-F cpDNA region was amplified using a Poaceae specific primer pair. The correlation between the quantity of DNA and pollen concentration was tested using R statistical programming language. Results: Although a significant correlation was obtained between the M1 and M2 methods (R2=0.655, p<0.01), the M2 method was more correlated with pollen concentration. The correlation between pollen and DNA content changed due to episodes that were observed during the pollen season. DNA concentrations from the PCR data were significantly correlated with pollen concentrations determined by light microscopy (R2=0.767, p<0.01) in episode II using the M2 method and during the entire season (R2=0.469, p<0.01) using M2. Conclusions: The M2 method correctly identified Poaceae pollen in mixed air samples from Zonguldak Province. The non-coding trnT-F cpDNA region was used for the first time in aerobiological samples to identify Poaceae pollen. Use of this method that does not require DNA extraction may be a crucial step for real-time pollen monitoring devices to be developed in the future. The correlation strength between pollen and amplified DNA content could be improved using a sampler that has a lower absorption rate, and a more sensitive technique, such as qPCR. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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36. A Serosurvey on Some Canine Vector-borne Zoonoses (Anaplasma spp., Ehrlichia spp., Borrelia burgdorferi, Dirofilaria immitis and Leishmania spp.) in Osmaniye.
- Author
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GÜVEN GÖKMEN, Tülin, GÜNAYDIN, Elçin, TURUT, Nevin, AKIN, Bünyamin, KOÇ, Özgür, and ÜTÜK, Armağan Erdem
- Subjects
- *
DIROFILARIA immitis , *ANAPLASMA , *BORRELIA burgdorferi , *EHRLICHIA , *ZOONOSES , *DISEASE prevalence , *ENZYME-linked immunosorbent assay , *SEROPREVALENCE - Abstract
Vector-borne diseases in dogs are of major global significance for their impact on animal and human health. Especially, it is necessary to determine the prevalence of agents found in reservoir animals by conventional, molecular and serological methods for the application of control programs for these diseases. Serosurvey studies are one of the reliable methods to know the presence and prevalence of these diseases in our country and region. In this study, it was aimed to detect the prevalence of Ehrlichia canis/E.ewingii, Anaplasma platys/A.phagocytophilum, Borrelia burgdorferi, Dirofilaria immitis and Leishmania infantum in dogs in Osmaniye. Five canine vector-borne diseases were investigated with a rapid in-clinic enzyme-linked immunosorbent assay in 100 clinically healthy dog serum samples in Osmaniye city center, Düziçi, Sumbas, Kadirli, Hasanbeyli, Bahçe and Toprakkale districts. Seroprevalence rate was detected as 3% for E.canis/E.ewingii and 1% for D.immitis by SNAP 4Dx PLUS. The prevalence of A.platys/A.phagocytophilum, Borrelia burgdorferi and L.infantum were determined as 0%. In conclusion, our study in which we determined the seroprevalence of dog vector-borne diseases in Osmaniye is the first study in which five agents are determined in one step and will contribute to the effective control programs prepared for animal and public health in our region. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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- View/download PDF
37. Molecular Survey of Hepatozoonosis in Natural Infected Dogs: First Detection and Molecular Characterisation of Hepatozoon canis in Kyrgyzstan.
- Author
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ALTAY, Kursat, AYDIN, Mehmet Fatih, AYTMIRZAKIZI, Ayperi, JUMAKANOVA, Zarima, CUNUSOVA, Ayday, and DUMANLI, Nazir
- Subjects
- *
CANIS , *PROTOZOAN diseases , *POLYMERASE chain reaction , *BLOOD sampling , *DOG diseases - Abstract
Canine hepatozoonosis is a tick-borne protozoan disease and widespread in Europe, Africa, Asia and America. There is not any available data about the presence of Hepatozoon infections in dogs in Kyrgyzstan. In the study we aimed that investigate the presence of Hepatozoon canis and the prevalance of Hepatozoon infections in dogs from Kyrgyzstan using polymerase chain reaction (PCR) and sequence analysis. To determine the prevelance of hepatozoonosis in dogs, a total of 170 blood samples were applied to PCR to amplify a fragment of 666 bp found in 18S SSU rRNA gene of Hepatozoon spp. The PCR results shown that Hepatozoon infection rate was 28.8% (49/170) in dogs. Eleven representative positive samples were sequenced to classification of the species. The nucleotide sequences were compared to the H. canis sequences which registered in GenBank using the basic local alignment search tool. Results of sequence analyse of 11 amplicons indicated that 8 were 100% identical and the other 3 sequences shared 99% similarity with H. canis. The sequences were deposited in Genbank with accession numbers from MG917709 to MG917719. It was the first record of H. canis in dogs in Kyrgyzstan. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
38. Viral Ensefalit / Menenjit Şüpheli Hastalarda,Beyin Omurilik Sıvısı Örneklerinde Herpes Simplex Virüslerinin Real-Time PZR ile Araştırılması.
- Author
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Terzi, Hüseyin Agah, Aydemir, Özlem, Karakeçe, Engin, Köroğlu, Mehmet, and Altındiş, Mustafa
- Abstract
Objective: Human herpes viruses have an important role in the pathogenesis of the central nervous system (CNS) infections seperating two general category as aseptic meningitis and encephalitis. In this study we aimed to investigate the frequency of Herpes Simplex Virus (HSV- 1/2) in cerebrospinal fluid (CSF), biochemical specifications and also bacteriological culture of CSF samples in suspected cases of viral infections of CNS in our region. Material-Method: In this study, 110 CSF samples sent to our laboratory from patients with suspected CNS infection between January 2016-December 2017 were evaluated retrospectively. HSV-1/2 DNA presence was evaluated qualitatively with Real-time PCR technique by using Artus® HSV ½ QS-RGQ (Qiagen, Hamburg, Germany) kits on Rotor-Gene system (Qiagen, Hamburg, Germany). Also, protein and glucose levels in CSF samples were recorded. Furthermore bacteriological culture of CSF samples were evaluated simultaneously. Results: While, HSV-1 DNA was detected by nucleic acid testing in 5 of the 110 patients (4.5%), HSV-2 DNA was not detected. The CSF glucose and protein levels were found normal in 42 (38%) and 27 (25%) patients, respectively. The CSF glucose levels of the patients, who were found to be positive for HSV-1 were high in four patients. Also CSF protein levels in HSV-1 positive group were detected high in 3 patients. HSV ½ negative 8 different samples were detected positive in bacteriological culture. Conclusions: Because of the high mortality and morbidity rates in CNS infections, initiating of specific antiviral treatment after the rapid identification of the agent is crucial. Even though encephalitis caused by herpes viruses were seen more frequent, other viral and bacterial agents must be kept in mind. Therefore necessity of moleculer methods are taking over like multiplex PCR which detects multiplexed viral and bacterial agents at the same time and in a short time. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
39. The prevalence of Cyclospora cayatenensis in patients with gastrointestinal system complaints.
- Author
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Ozpinar, Necati and Karaman, Ulku
- Subjects
- *
GASTROINTESTINAL system , *WATER sampling , *PARASITOLOGY , *SIMULATED patients , *PROTOZOA - Abstract
Objective: In our study, the prevalence of Cyclospora cayatenensis was investigated molecularly in patients with gastrointestinal complaints. Method: Seventy patients with gastrointestinal system complaints were included in the study. Nested PCR was applied to all samples taken from patients. Results: In this study, 70 stool samples taken from the patients with gastrointestinal system complaints and sent to the parasitology laboratory of Ordu University Faculty of Medicine were examined molecularly for Cyclospora cayatenensis and of the 70 samples, 25 (35.7%) were found to be positive. Conclusions: The high rates determined from the patient samples and the environmental samples with the studies suggest that this parasite may be the cause of an epidemic in the future. Especially diarrhea patients, water and food samples should be evaluated in terms of coccidian protozoa like Cyclospora. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
40. P0-Related Protein Accelerates Human Mesenchymal Stromal Cell Migration by Modulating VLA-5 Interactions with Fibronectin
- Author
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Maria G. Roubelakis, Grigorios Tsaknakis, Feng-Juan Lyu, Ourania Trohatou, Andrew C. W. Zannettino, and Suzanne M. Watt
- Subjects
human bone marrow stromal cells ,PZR ,SHP-2 ,cell adhesion molecules ,fibroblasts ,ITIM ,Cytology ,QH573-671 - Abstract
P0-related protein (PZR), a Noonan and LEOPARD syndrome target, is a member of the transmembrane Immunoglobulin superfamily. Its cytoplasmic tail contains two immune-receptor tyrosine-based inhibitory motifs (ITIMs), implicated in adhesion-dependent signaling and regulating cell adhesion and motility. PZR promotes cell migration on the extracellular matrix (ECM) molecule, fibronectin, by interacting with SHP-2 (Src homology-2 domain-containing protein tyrosine phosphatase-2), a molecule essential for skeletal development and often mutated in Noonan and LEOPARD syndrome patients sharing overlapping musculoskeletal abnormalities and cardiac defects. To further explore the role of PZR, we assessed the expression of PZR and its ITIM-less isoform, PZRb, in human bone marrow mesenchymal stromal cells (hBM MSC), and its ability to facilitate adhesion to and spreading and migration on various ECM molecules. Furthermore, using siRNA knockdown, confocal microscopy, and immunoprecipitation assays, we assessed PZR and PZRb interactions with β1 integrins. PZR was the predominant isoform in hBM MSC. Migrating hBM MSCs interacted most effectively with fibronectin and required the association of PZR, but not PZRb, with the integrin, VLA-5(α5β1), leading to modulation of focal adhesion kinase phosphorylation and vinculin levels. This raises the possibility that dysregulation of PZR function may modify hBM MSC migratory behavior, potentially contributing to skeletal abnormalities.
- Published
- 2020
- Full Text
- View/download PDF
41. Self-Compacted Concrete with Self-Protection and Self-Sensing Functionality for Energy Infrastructures
- Author
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Alonso Maria Cruz and Puentes Javier
- Subjects
scc ,self-diagnosis ,electrical resistivity ,pzr ,cnt ,cmf ,thermal fatigue ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
This paper aims to demonstrate the self-protection and self-sensing functionalities of self-compacted concrete (SCC) containing carbon nanotubes (CNT) and carbon microfibers (CMF) in a hybrid system. The ability for self-sensing at room temperature and that of self-protection after thermal fatigue cycles is evaluated. A binder containing a high volume of supplementary mineral additions (30%BFSand20%FA) and different type of aggregates (basalt, limestone, and clinker) are used. The self-diagnosis is assessed measuring electrical resistivity (ER) and piezoresistivity (PZR) in compression mode within the elastic region of the concrete. Thermal fatigue is evaluated with mechanical and crack measurements after heat cycles (290−550 °C). SCC withstands high temperature cycles. The protective effect of the hybrid additive (CNT+CMF) notably diminishes damage by keepinghigher residual strength and lessmicrocracking of the concrete. Significant reductions in ER are detected. The self-diagnosis ability of functionalized SCC isconfirmed with PZR. A content of the hybrid functional additive (CNT+CMF) in the percolation region is recommended to maximize the self-sensing sensitivity. Other parameters as sample geometry, sensor location, power supply, and load level have less influence.
- Published
- 2020
- Full Text
- View/download PDF
42. Bir Kıl Keçisinde İntrakardiyak Hidatik Kistin Moleküler Karakterizasyonu ve Patolojisi.
- Author
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AKDENİZ İNCİLİ, Canan, GÜNYAKTI KILINÇ, Şeyma, KARABULUT, Burak, ÇEVİK, Aydın, TİMURKAAN, Necati, ÇELİK, Figen, and ERÖKSÜZ, Hatice
- Subjects
LEFT heart ventricle ,ECHINOCOCCOSIS ,ECHINOCOCCUS granulosus ,ENDOCARDIUM ,AUTOPSY ,MYOCARDIUM - Abstract
Copyright of Firat Universitesi Saglik Bilimleri Veteriner Dergisi is the property of Firat Universitesiu, Saglik Bilimleri Enstitusu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
43. HELAL VE HELAL OLMAYAN GIDA BİLEŞENLERİNİN TESPİTİNDE DNA TABANLI ANALİZ YÖNTEMLERİNDEN POLİMERAZ ZİNCİR REAKSİYONUNUN (PZR) KULLANILMASI
- Author
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ALKAY, Zühal
- Subjects
Helal gıda ,Biyoteknoloji ,PZR ,taklit-tağşiş ,Food Science and Technology ,Gıda Bilimi ve Teknolojisi - Abstract
Halal foods and their quality is of great importance in the daily lives of Muslims. One of the main challenges of halal foods is the detection of food adulteration and the presence of any of the prohibited ingredients which is often undetectable and undetectable with the naked eye. Therefore, thanks to the awareness of Muslim consumers who are concerned about the authenticity of food products, various methods have been used to verify the halal ingredients in the foods produced around the world. Sensitive, easy-to-use and reliable methods have been preferred to provide halal authentication of food products rapidly. The most common of these techniques is Polymerase Chain Reaction (PCR). This method pro-vides several advantages. Consequently, the review deals with recent studies on the PCR technique used in the analysis of halal food authenticity., Helal gıda ve kalitesi, Müslümanların günlük yaşamlarında büyük önem taşımaktadır. Helal gıdaların kullanımında karşılaşılan ana zorluklarından biri, gıda tağşişinin tespiti ve yasak bileşenlerin varlığının genellikle fark edilememesi veya çıplak gözle belirlenememesidir. Bu yüzden helal gıda ürünlerinin güvenirliği konusunda endişelenen Müslüman tüketicilerin bilinçlenmesi ile gıdalarda yer alan bileşenlerin doğrulanmasında çeşitli yöntemler kullanılmaya başlanmıştır. Gıda ürünlerinin helal uygunluk değerlendirilmesinin hızlı bir şekilde sağlanabilmesi için hassas, kullanımı kolay ve güvenilir yöntemler tercih edilmektedir. Bu tekniklerden en yaygın olanı, DNA analizlerinde kullanılan Polimeraz Zincir Reaksiyonu (PZR) yöntemidir. Bu yöntemin analizlerde kullanılması da çok çeşitli avantajlar sağlamaktadır. Bu derleme çalışmasında, gıda bileşenlerinin helal orijinli olup olmadıklarının belirlenmesinde kullanılan PZR tekniği ve yapılan son çalışmalar ele alınmıştır.
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- 2022
44. The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal Swab Samples from Dogs in Van Province, Turkey.
- Author
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KARAPINAR, Zeynep, DİNÇER, Ender, and ÖZKAN, Cumali
- Subjects
- *
PHYLOGENY , *PARVOVIRUSES , *MYOCARDITIS , *DOG diseases , *VACCINATION - Abstract
In this study, it was aimed to determine the presence of CPV infection in the blood and rectal swaps of suspected dogs that they were handed to Yuzuncu Yıl University, Faculty of Veterinary, Department of Internal Disease using PCR techniques and molecular characterization of local viruses. 57 (75%) of the 76 rectal swabs used in the study and 40 (64%) of the 62 blood samples were determined to be positive by PCR obtained from dogs with clinical symptoms. Selected samples were positive with the virus carried the molecular characterization. The results of the sequence analysis were similar among themselves and they were found in CPV-2a and CPV-2b groups. It was determined that the sequences of virus strains showed 99-100% homology with other strains obtained from Genbank. [ABSTRACT FROM AUTHOR]
- Published
- 2018
45. Evaluation of Toxoplasma gondii Molecular Test Results in Patients Admitted to Ankara City Hospital: Three-Year Retrospective Analysis
- Author
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Filiz DEMİREL and Fisun KIRCA
- Subjects
Toxoplasma gondii ,Toksoplazmoz ,PZR ,HIV ,Health Care Sciences and Services ,Geography, Planning and Development ,Toxoplasmosis ,PCR ,Management, Monitoring, Policy and Law ,Sağlık Bilimleri ve Hizmetleri - Abstract
Amaç: Toxoplasma gondii dünya nüfusunun yaklaşık %25-30'unu enfekte eder. Toksoplazmoz bağışıklık sistemi sağlam bireylerde genellikle asemptomatiktir, ancak enfeksiyon konjenital enfeksiyonlu çocuklarda ve immunsupresif bireylerde hayatı tehdit edici olabilir. Bu çalışmada toksoplazmozdan şüphelenilen hastaların moleküler test sonuçlarının geriye dönük olarak incelenmesi amaçlandı.Gereç ve Yöntem: Hastanemizde 2019-2022 yılları arasındaki üç yıllık dönemde gerçek zamanlı PCR ile T. gondii DNA araştırılan toplam 647 klinik örnek geriye dönük olarak değerlendirildi. T. gondii'nin kalitatif tespiti için ticari bir DNA ekstraksiyon kiti (Qiagen, Almanya) ve real time PCR kiti (Sacace Biotechnologies, İtalya) kullanılarak DNA izolasyonu ve DNA amplifikasyonu yapıldı. Hastaların demografik ve klinik parametreleri ile ilgili veriler laboratuvar bilgi yönetim sisteminden elde edildi.Bulgular: Real-time PCR ile T. gondii DNA'sı araştırılan 647 hastanın %51,8'i kadındı ve hastaların ortalama yaşı 37,03 idi. Hastalardan beşinde, real-time PCR ile T. gondii DNA pozitif tespit edildi ve PCR pozitifliği analiz edilen tüm örnekler içinde %0,8 bulundu. En fazla pozitiflik tespit edilen klinik örnek kandı (%80). T. gondii DNA pozitifliği saptanan beş hastadan birine konjenital toksoplazmoz, dördünde HIV enfeksiyonu vardı.Sonuç: Özellikle bağışıklık sistemi baskılanmış hastalarda toksoplazmozun hızlı ve doğru teşhisi, hızlı ve spesifik tedavi için çok önemlidir. Toksoplazmoz tanısında serolojik testlere ek olarak moleküler testlerin öneminin anlaşılabilmesi için ileri çalışmalara ihtiyaç vardır., Aim: Toxoplasma gondii infects about 25-30% of the world population. Toxoplasmosis is generally asymptomatic in immunocompetent individuals, but the infection can be life threatening in congenitally infected children and immunocompromised individuals. In this study, it is aimed to analyse the molecular test results of patients suspected with toxoplasmosis, retrospectively.Material and Method: A total of 647 clinical samples investigated for T. gondii DNA with real-time PCR during the three-year period between 2019 and 2022 were evaluated retrospectively. For the qualitative detection of T. gondii, DNA isolation and DNA amplification were performed using commercial DNA extraction kit (Qiagen, Germany) and real time PCR kit (Sacace Biotechnologies, Italy), respectively. The data on the demographic and clinical parameters of the patients were obtained from the laboratory information management system.Results: Out of 647 patients investigated for T. gondii DNA with real-time PCR, 51.8% were female and the mean age of the patients was 37.03 years. Among all patients, five were positive for T. gondii DNA with real-time PCR and the frequency of a positive PCR result was found 0.8% of all samples analysed. The most frequently positive clinical sample was blood (80%). Among five patients with T. gondii DNA positivity, one was diagnosed with congenital toxoplasmosis, four were HIV-infection.Conclusion: Fast and accurate diagnosis of toxoplasmosis especially in immunosuppressed patients is crucial for rapid and specific treatment. Further studies are needed to understand the importance of molecular tests, in addition to the serological tests, in the diagnosis of toxoplasmosis.
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- 2022
46. Detection of Salmonella, Shigella and Campylobacter Species in Clinical Samples of Patients with Acute Gastroenteritis by Culture and Molecular Method
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AVCİ, Gamze, KİRAZ, Nuri, DURAN, Hülya, and ERDAL, Berna
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Campylobacter ,Salmonella ,Shigella ,PZR ,Health Care Sciences and Services ,PCR ,Sağlık Bilimleri ve Hizmetleri - Abstract
Akut gastroenterit (AGE), tüm dünyada yaygın olarak görülen enfeksiyon hastalıkları arasındadır. Campylobacter, Salmonella, Shigella türleri AGE’nin bakteriyel etkenleri arasında ilk sıralarda bulunmaktadır. Bu çalışmada, laboratuvarımıza gönderilen gaita örneklerinde kültür yöntemi ve moleküler yöntemle Campylobacter, Salmonella ve Shigella sıklığını saptamak ve her iki yöntemin kıyaslanması amaçlanmıştır. 2019-2020 yılları arasında Namık Kemal Üniversitesi Tıp Fakültesi Hastanesi’ne ishal yakınması ile başvuran, ayaktan erişkin ve çocuk hasta örnekleri dahil edilmiştir. Dışkı örnekleri Campylobacter, Salmonella ve Shigella türlerinin tespiti için konvansiyonel kültür yöntemleri ve polimeraz zincir reaksiyonu (PZR) yöntemi ile çalışılmıştır. Çalışmamızda 400 (%77 yetişkin, %23 çocuk) hastaya ait dışkı örneği değerlendirilmiştir. Örneklerin kültür ile değerlendirilmesinde 14 örnekte (%3.5) etken saptanmış; 10’u (%2.5) Campylobacter spp., 4’ü (%1) Salmonella spp. olarak tiplendirilmiş, Shigella spp. izole edilememiştir. Kültür yöntemi ile Campylobacter spp. ve Salmonella spp. sıklığı çocuklarda sırasıyla %6.5 ve %2.2, yetişkinlerde %1.3 ve %0.7 olarak saptanmıştır. Örneklerin PZR ile değerlendirilmesinde 38 örnekte (%9.6) etken saptanmış; 33’ünde (%8.3) Campylobacter spp., 5’inde (%1.3) Salmonella spp. varlığı tespit edilmiş, hiçbir örnekte Shigella spp. saptanamamıştır. Kültürde üreme saptanan 14 dışkı örneğinin tamamında PZR yönteminde de aynı etken mikroorganizma tespit edilmiştir. 24 örnekte ise kültürde üreme saptanmayıp sadece PZR’de Campylobacter spp. veya Salmonella spp. saptanmıştır. Gaita kültürü AGE tanısında altın standart yöntem olmasına rağmen araştırmamız sonucunda PZR’nin hem hızlı sonuç vermesi hem de saptama oranının yüksek olması nedeniyle kültüre avantajlı olduğu görülmüştür. Bu nedenle moleküler yöntemlerin rutin tanıda kullanılmasının faydalı olacağı kanaatindeyiz., Acute gastroenteritis (AGE) is among the most common infectious diseases all over the world. Campylobacter, Salmonella and Shigella species are among the first bacterial agents of AGE. In this study, it was aimed to determine the prevelance of Campylobacter, Salmonella and Shigella by culture method and molecular method in stool samples sent to our laboratory and to compare both methods. Samples of adult and pediatric patients who applied to the Namık Kemal University Medical Faculty Hospital with the complaint of diarrhea between 2019-2020 were included. Stool samples were studied by conventional culture methods and polymerase chain reaction (PCR) method for the detection of Campylobacter, Salmonella and Shigella species. In our study, stool samples of 400 (77% adults, 23% children) patients were evaluated. In the evaluation of the samples by culture, agents were detected in 14 samples (3.5%), 10 (2.5%) were Campylobacter spp., 4 (1%) were Salmonella spp, Shigella spp. could not be isolated. By culture method, Campylobacter spp. and Salmonella spp. prevalence was 6.5% and 2.2% in children, and 1.3% and 0.7% in adults, respectively. In the evaluation of the samples by PCR, the causative agent was found in 38 samples (9.6%); Campylobacter spp. was found in 33 (8.3%) and Salmonella spp. was found in 5 (1.3%) samples, Shigella spp. not detected. The same causative microorganism was detected in the PCR method in all 14 stool samples with growth in the culture. In 24 samples, no growth was detected in the culture, only by PCR method, Campylobacter spp. or Salmonella spp. detected. Although stool culture is the gold standard method in the diagnosis of AGE, as a result of our research, PCR was found to be advantageous to culture because of its rapid results and high detection rate. Therefore, we believe that it would be beneficial to use molecular methods in routine diagnosis.
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- 2022
47. Investigation of Viruses Causing Gastroenteritis by Immunochromatographic Tests and Multiplex PZR Method
- Author
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TOPÇU, Mücahide, KÖROĞLU, Mehmet, AYDEMİR, Özlem, AYDEMİR, Yusuf, ALTINDİŞ, Mustafa, and ELMAS, Bahri
- Subjects
Gastroenterit ,Rotavirus ,Adenovirus ,Norovirus ,PCR ,Medicine ,PZR ,Tıp - Abstract
Amaç: Akut gastroenterit (AGE), erken ve doğru tedavi edilmediğinde özellikle çocuklarda yüksek morbidite ve mortalite ile seyredebilen ve tüm dünyada sık görülen bir enfeksiyondur. Çocukluk döneminde en sık Rotavirüsler, Adenovirüsler, Norovirüsler AGE’lere neden olmaktadır. Viral enfeksiyonların hızlı tanısında, immünokromatografik (ICT) veya ELISA temelli yöntemler tercih edilmekte iken, son yıllarda multipleks polimeraz zincir reaksiyonu (PZR) testleri de laboratuvarlarda kullanılmaya başlanmıştır. Bu çalışmada Rotavirus, Adenovirus ve Norovirus tanısında immünokromatografik yöntem ve PZR yöntemi ile elde edilen sonuçların karşılaştırılması amaçlanmıştır.Gereç ve Yöntemler: Çalışmaya AGE tanılı hastaların ICT yöntemi ile Rotavirus ve/veya Adenovirus pozitif olarak saptanan 67 gaita örneği ve ICT yöntemi ile viral, bakteriyel, paraziter etkenler açısından negatif bulunan 17 gaita örneği dâhil edildi. Bu örneklerde Rotavirus, Norovirus RNA’sı ile Adenovirus DNA’sı multipleks PZR kiti kullanılarak araştırıldı. Bulgular: ICT yöntemi ile örneklerin 62’sinde (%73,8) Rotavirus pozitif iken, gerçek zamanlı PZR yöntemi ile 49’unda (%58,5) Rotavirus pozitif bulundu. ICT yöntemi ile örneklerin beşinde (%5,9) Adenovirus pozitif bulunurken, multipleks gerçek zamanlı PZR yöntemi ile 40’ında (%47,6) Adenovirus pozitif tespit edildi. Örneklerin 11’inde (%13) Norovirus pozitif saptandı.Sonuç: En sık karşılaşılan viral gastroenterit etkenleri olan Rotavirus ve Adenovirus tanısı için çok yaygın olarak ICT kullanılmaktadır. Ancak çalışmamızda da görüldüğü üzere; bu tür olgularda Norovirüs gibi diğer gastroenterit etkeni virüslerin de klinik tablodan sorumlu olabileceği göz ardı edilmemelidir. Rotavirus ve Adenovirus tanımlamasında ICT ve PZR yöntemleri arasında anlamlı ancak düşük düzeyde korelasyon saptandı., Aim: Acute gastroenteritis (AGE) is a common infection all over the world, which can progress with high morbidity and mortality, especially in children, if not treated early and correctly. Rotaviruses, Adenoviruses, Noroviruses most commonly cause AGEs in childhood. While immunochromatographic (ICT) or ELISA-based methods are preferred for rapid diagnosis of viral infections, multiplex polymerase chain reaction (PCR) tests have also been used in laboratories in recent years. In this study, it is aimed to compare the results obtained by immunochromatographic and real-time PCR methods in the diagnosis of Rotavirus, Adenovirus and Norovirus.Material and Methods: The study included 67 stool samples of a patient diagnosed with AGE who were positive for Rotavirus and/or Adenovirus by the ICT method, and 17 stool samples that were found to be negative in terms of viral, bacterial and parasitic agents by the ICT method. Rotavirus, Norovirus RNA and Adenovirus DNA were investigated in all samples using multiplex real-time PCR kit.Results: At least one of Rota/Adeno was found to be positive in 67 of the stool samples of 84 patients included in the study. While 62 (73.8%) of the samples were Rotavirus positive with the ICT method, 49 (58.5%) were positive with the real-time PCR method. While 5 (5.9%) of the samples were found to be Adenovirus positive with the ICT method, 40 (47.6%) of them were found positive with the real-time PZR method. Norovirus was positive in 11 (13%) of the samples. Conclusion: ICT is widely used for the diagnosis of the most common viral gastroenteritis agents Rotavirus and Adenovirus. However, as can be seen in our study; In such cases, it should not be ignored that other gastroenteritis-causing viruses, such as other Norovirus, may be responsible for the clinical picture. A significant but low level correlation was found between ICT and PCR methods in the identification of Rotavirus and Adenovirus.
- Published
- 2022
48. Aydın Bölgesinde Kum Havuzu Bulunan Parklarda Zoonoz Helmintlerin Yaygınlığının Belirlenmesi
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Şahin, Tayfun, Aypak, Süleyman, Aydın Adnan Menderes Üniversitesi Sağlık Bilimleri Enstitüsü Veterinerlik Parazitolojisi, and Aydın Adnan Menderes Üniversitesi, Sağlık Bilimleri Enstitüsü, Veterinerlik Parazitolojisi Doktora Programı
- Subjects
Kum/toprak ,PCR ,Sand/soil ,Çocuk parkı, Echinococcus, Kum/toprak, PZR, Toxocara ,Child playground ,PZR ,Çocuk parkı ,Echinococcus ,Toxocara - Abstract
Amaç: Bu çalışmada Echinococcus spp. ve Toxocara spp.’nin Aydın Bölgesi çocuk oyun parklarındaki yaygınlığının modifiye yüzdürme ve PZR yöntemi kullanarak tespit edilmesi amaçlanmıştır. Gereç ve Yöntem: Çalışma Aydın’ın yedi ilçesini kapsamaktadır. Efeler’de 9, Nazilli’de 8, Söke’de 8, Çine’de 7, Bozdoğan, Germencik ve Kuşadası’nda 6’şar, toplam 50 park, her parktan 5 örnek alınarak toplam 250 örnek incelenmiştir. Ek olarak toplam 81 dışkı örneği de çalışmaya dahil edilmiştir. Bulgular: Yapılan mikroskobik incelemelerde toplanan 250 örneğin 17’si (%6,8) en az bir parazit türü yönünden pozitif, 233’ü (%93,2) negatif olarak tespit edilmiştir. Pozitif 17 örneğin, 8’inde (%3,2) Toxocara spp., 9’unda (%3,6) Strongylid tip yumurtalar tespit edilmiştir. Efeler’deki parkların %33 (%11 Strongylid tip yumurta, %22 Toxocara spp.), Bozdoğan’daki parkların %67 (%33 Toxocara spp., %33 Strongylid tip yumurta), Kuşadası’daki parkların %33 oranında (%100 Toxocara spp.) pozitif olduğu tespit edilmiştir. Mikroskobik incelemelerde, Taeniid tip yumurtaya rastlanmamıştır. 250 örneğin PZR analizinde, E. granulosus bakımından 16 (%6,4) ve T. cati bakımından 46 (%18,4) örneğin pozitif olduğu görüldü. 50 parkın PCR analizi sonucunda 30’u (%60) en az bir parazit türü ile, 21’i (%42) T. cati, 7’si (%14) E. granulosus ve 2’si (%4) hem T. cati hem de E. granulosus yönünden pozitif olduğu tespit edildi. Toplanan 81 dışkıdan 11'i (%13,6) Toxocara spp. (%8,6), Taenia spp. (%2,6), Strongylid (%1,2) ve Ascaris lumbricoides (%1,2) yönünden pozitif bulundu. Sonuç: Bu sonuçlara göre kum havuzu bulunan parklar çocuklar için büyük riskler barındırmaktadır. Yapılan analizlerde elde edilen sonuçlar kum/toprak örneklerinin incelenmesinde sadece mikroskobik bakı ya da PZR’ın tek başına yeterli olmadığını, her iki yöntemin birlikte kullanılması gerektiğini göstermektedir. Bu tez Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından VTF-15029 proje numarası ile desteklenmiştir. KABUL VE ONAY i TEŞEKKÜR ii İÇİNDEKİLER iii SİMGELER ve KISALTMALAR DİZİNİ vi ŞEKİLLER DİZİNİ viii RESİMLER DİZİNİ ix TABLOLAR DİZİNİ x ÖZET xii ABSTRACT xiii 1. GİRİŞ 1 2. GENEL BİLGİLER 4 2.1. Topraktan Bulaşabilen Önemli Zoonoz Helmint Türleri ve Yaygınlıkları 4 2.1.1. Cestod Enfeksiyonları 4 2.1.1.1. Kistik Echinococcosis (KE) 4 2.1.1.2. Alveolar Echinococcosis (AE) 7 2.1.1.3. Echinococcus Türlerinin Yumurta Özellikleri 8 2.1.1.4. Echinococcus Türlerinin Dünyadaki ve Türkiye’deki Yayılışı 10 2.1.1.4.1. Kistik Echinococcosis’in Yayılışı 10 2.1.1.4.2. Alveolar Echinococcosis’in Yayılışı 14 2.1.2. Nematod Enfeksiyonları 16 2.1.2.1. Ancylostomatidosis 17 2.1.2.1.1. Kancalıkurt Türlerinin Yumurta Özellikleri 19 2.1.2.1.2. Kancalıkurtların Dünyadaki ve Türkiye’deki Yayılışı 20 2.1.2.1.3. Cutaneous Larva Migrans’ın (CLM) Dünya’daki ve Türkiye’deki Yaygınlığı 23 2.1.2.2. Toxocarosis ve Toxascariosis 24 2.1.2.2.1. Toxocara Türlerinin Yumurta Özellikleri 28 2.1.2.2.2. Toxocara Türlerinin Dünya’daki ve Türkiye’deki Yayılışı 31 2.1.2.2.3. Visceral Larva Migrans’ın Yaygınlığı 34 2.2. Parklarda, Çocuk Oyun Alanlarında ve Kamusal Alanlarda Zoonoz Helmint Yumurtalarının Yaygınlığı 35 2.2.1. Dünyada Farklı Ülkelerdeki Çocuk Oyun Alanlarının/Parkların ya da Kamusal Alanların Köpek-Kedi Helmint Yumurtaları ve Diğer Parazitlerle Kontaminasyon Durumu 35 2.2.2. Türkiye’de Farklı Şehirlerdeki Çocuk Oyun Alanlarının/Parkların ya da Kamusal Alanların Kedi-Köpek Helmint Yumurtaları ve Diğer Parazitlerle Kontaminasyon Durumu 40 2.3. Kullanılan Yöntemler 45 2.4. Çalışma Sonuçlarını Etkileyebilecek Faktörler 45 2.4.1. İncelenen Toprak Örneği Miktarı 46 2.4.2. İncelenen Toprağın Türü ve Güneşe Maruz Kalma Durumu 46 2.4.3. İncelenen Toprağa Flotasyon Öncesi Uygulanan İşlemler 47 2.4.4. Tercih Edilen Flotasyon Çözeltileri 47 2.5. Moleküler Yöntemler 48 3. GEREÇ VE YÖNTEM 50 3.1. Gereç 50 3.1.1. Kum/Toprak ve Dışkı Örneklerinin Toplanması 50 3.2. Yöntem 55 3.2.1. Toplanan Toprak/kum Örneklerinin Hazırlanması ve Mikroskobik Olarak İncelenmesi 55 3.2.2. Toplanan Dışkı Örneklerinin Hazırlanması ve İncelenmesi 56 3.2.3. Toplanan Örneklerin PZR ile Moleküler Düzeyde İncelenmesi 56 3.2.4. Pozitif PZR Ürünlerinin Sekans Analizleri İçin Klonlanması 58 3.2.5. İstatistik Analiz 60 4. BULGULAR 61 4.1. Kum/toprak Örneklerinin Mikroskobik Bakıda Elde Edilen Sonuçları 61 4.2. PZR Analizi ile Tespit Edilen Parazitler 64 4.3. Dizilim Sonuçları 71 4.4. Parklardan Toplanan Dışkı Örneklerinin Mikroskobik Bakı Sonuçları 73 5. TARTIŞMA 76 6. SONUÇ VE ÖNERİLER 89 KAYNAKLAR 90 EKLER 115 Ek 1. Kum/toprak ve dışkı örneği toplanan parklarda mikroskobik bakı ve PZR ile elde edilen sonuçlar. 115 BİLİMSEL ETİK BEYANI 118 ÖZ GEÇMİŞ 119
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- 2022
49. The use of polymerase chain reaction (pcr), one of the DNA-based analysis methods in the detection of halal and non-halal food components
- Author
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Alkay, Zühal
- Subjects
Adulteration ,Halal food ,PCR ,Helal gıda ,PZR ,Biyoteknoloji ,Taklit-tağşiş ,Biotechnology - Abstract
Helal gıda ve kalitesi, Müslümanların günlük yaşamlarında büyük önem taşımaktadır. Helal gıdaların kullanımında karşılaşılan ana zorluklarından biri, gıda tağşişinin tespiti ve yasak bileşenlerin varlığının genellikle fark edilememesi veya çıplak gözle belirlenememesidir. Bu yüzden helal gıda ürünlerinin güvenirliği konusunda endişelenen Müslüman tüketicilerin bilinçlenmesi ile gıdalarda yer alan bileşenlerin doğrulanmasında çeşitli yöntemler kullanılmaya başlanmıştır. Gıda ürünlerinin helal uygunluk değerlendirilmesinin hızlı bir şekilde sağlanabilmesi için hassas, kullanımı kolay ve güvenilir yöntemler tercih edilmektedir. Bu tekniklerden en yaygın olanı, DNA analizlerinde kullanılan Polimeraz Zincir Reaksiyonu (PZR) yöntemidir. Bu yöntemin analizlerde kullanılması da çok çeşitli avantajlar sağlamaktadır. Bu derleme çalışmasında, gıda bileşenlerinin helal orijinli olup olmadıklarının belirlenmesinde kullanılan PZR tekniği ve yapılan son çalışmalar ele alınmıştır. Halal foods and their quality is of great importance in the daily lives of Muslims. One of the main challenges of halal foods is the detection of food adulteration and the presence of any of the prohibited ingredients which is often undetectable and undetectable with the naked eye. Therefore, thanks to the awareness of Muslim consumers who are concerned about the authenticity of food products, various methods have been used to verify the halal ingredients in the foods produced around the world. Sensitive, easy-to-use and reliable methods have been preferred to provide halal authentication of food products rapidly. The most common of these techniques is Polymerase Chain Reaction (PCR). This method pro-vides several advantages. Consequently, the review deals with recent studies on the PCR technique used in the analysis of halal food authenticity.
- Published
- 2022
50. Meme Kanseri Hastalarında hTERT Gen Ekspresyonunun Klinikopatolojik Önemi
- Author
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Kankaya, Burak, Tatar, Zeynep, and Dirican, Ebubekir
- Subjects
ÖR ,luminal ,Meme kanseri ,bazal benzeri ,PZR ,hTERT - Abstract
Amaç: Bu çalışma, meme kanserinde insan telomeraz ters transkriptaz (hTERT) genin mRNA ekspresyonunun klinik önemini incelemeyi ve klinikopatolojik rolünü araştırmayı amaçlamıştır. hTERT geni hücre proliferasyonu, apoptoz ve hücre invazyonu gibi olaylarda rol almaktadır. Yüksek hTERT mRNA ekspresyonunun meme kanseri, mide, akciğer, glioblastoma, baş ve boyun gibi solid tümörlerde kötü prognoz ile ilişkili olduğu bildirilmektedir. Gereç ve Yöntemler: 59 meme kanser ve sağlıklı kan örneklerinin RNA izolasyonları gerçekleştirildi. hTERT geni mRNA ekspresyonu Gerçek Zamanlı Polimeraz Zincir Reaksiyonu (RT-PZR) yöntemi ile analiz edildi. mRNA ekspresyon sonuçları 2-??ctmetoduyla kanserli ve sağlıklı örneklerde değerlendirildi. Klinik veriler toplandı ve bu verilerin hTERT gen ekspresyonu ile ilişkisi istatiksel olarak değerlendirildi. Bulgular: Meme kanser hastalarında hTERT geni mRNA ekspresyonu sağlıklı örneklere kıyasla anlamlı derecede yüksekti (p=0,0211). Düşük hTERT geni ekspresyonu östrojen reseptör (ÖR) (+) ve insan epidermal büyüme faktör reseptörü 2 (HER 2) (+) olan hastalarda anlamlı derecede ilişkiliydi (sırasıyla p=0,0464; p=0,0080). hTERT geni ekspresyonu histolojik derece (grade) 3 hastalarda derece 2’ye kıyasla yüksek anlamlı bulundu (p=0,0389). Bununla birlikte hTERT ekspresyonun bazal benzeri histolojik tipte luminal A ve luminal B’ye kıyasla anlamlı derecede yüksek olduğu gösterildi (sırasıyla p=0,0160; p=0,0008). Sonuç: Çalışmamızın bulguları, meme kanser hastalarında hTERT gen ekspresyonunun sağlıklı örneklere göre anlamlı olarak daha yüksek olduğunu gösterdi. Anormal hTERT gen ekspresyonu kanserojenez veya teşhiste önemli bir rol oynayabilir.
- Published
- 2022
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