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• The red color of the dragon fruit pulp and peel is due to the accumulation of betacyanins. • The betacyanin accumulated calli were encapsulated using sodium alginate and calcium chloride. • The synseeds were cold stored for 6 months at 4.0 °C temperature. • The synseeds were germinated and calli formed on nutrient media. • The betacyanin-loaded synseeds could be stored, transported, and directly used as inoculum for cell cultures to produce betacyanin. The red color of dragon fruit pulp and peel is due to the accumulation of betacyanins. In the present study, callus cultures were established using in vitro germinated seedlings of the white dragon fruit plant (Selenicereus undatus). Murashige and Skoog (MS) medium with 6-benzylaminopurine (BAP) at 2.0 mg/L was used to induce red-colored friable callus (92.0 % response). The betacyanin accumulated calli were harvested and encapsulated using sodium alginate (NaC 6 H 7 O 6) and calcium chloride (CaCl 2). These synseeds were cold stored for 6 months at 4.0 °C temperature. The stored synseeds were germinated on 2.0 mg/L BAP in the medium. The highest germination rate (88 %) of synseeds was achieved with 3 % sodium alginate polymerized with 75 mM calcium chloride, resulting in firm, uniform, and round synseeds. The betacyanin-loaded synseeds could be stored, transported, and directly used as inoculums for the extensive proliferation of calli and cell cultures for the commercial production of betacyanin. [ABSTRACT FROM AUTHOR]

This study examines the use of dragon fruit peel (DFP) powder as a dietary supplement on growth performance, immune responses, and gene expression of Nile tilapia, Oreochromis niloticus, cultured within biofloc systems. A total of 300 Nile tilapia fingerlings (14.64 ± 0.09 g) were subjected to five dietary treatments, with DFP added to a basal diet at doses of 0, 20, 40, 80, and 160 g kg−1 DFP. Growth and immunological responses were assessed after 4 and 8 weeks of feeding, and the transcriptional level of immune and antioxidant‐related genes was measured after 8 weeks. Fish fed diets containing DFP exhibited significantly greater weight gain, faster growth, and enhanced levels of key indicators of immunity than control fish (p < 0.05). A diet containing 40 g DFP kg−1 produced the best result in terms of growth, enhanced immune response indicators in skin mucus and blood serum, and the upregulation of IL‐1, IL‐8, LBP, GPx, GST‐α, and GSR expression (p < 0.05). Based on the quadratic regression analysis, the optimum concentration of DFP was 80 g kg−1. These findings collectively suggest that powdered DFP may serve as a promising feed supplement for Nile tilapia raised in biofloc systems. [ABSTRACT FROM AUTHOR]

In Nepal, dragon fruit farming is promising but faces limited operation due to its high initial cost during the establishment years. However, its market demand and export potential could provide an economic opportunity for farmers. Here, dragon fruit farmers were categorized as 39 small-scale and 25 large-scale farmers based on an average farm size of 20 kattha (6772.6 m2). A total of 64 dragon fruit farmers were selected randomly from different clusters through a multi-stage sampling technique. The results revealed that the average cost of dragon fruit production per kattha during the establishment year was NRs 259098.58 (1941.89 USD). The gross benefit was NRs 540972.00 (4054.98 USD) in a large-scale farm and NRs 402928.33 (3019.87 USD) in a small-scale farm. Also, an average BC ratio of 1.87 indicated a profitable farming business. Provision of extension services and cooperative engagement were significant among socioeconomic characteristics for the farm category. Land preparation, manure, labor, fertilizers, and plant protection costs were substantial parts of the total cost incurred in dragon fruit cultivation. Pillar and plant protection costs were positively significant in the production function analysis, and the cost of irrigation was negatively significant to gross returns. These findings will encourage the adoption of dragon fruit farming by providing farmers with essential information on production costs and profitability. Market stability, training, extension, and subsidies should be top priorities in authoritative management. [ABSTRACT FROM AUTHOR]

Near infrared (NIR) phosphors have been booming as promising candidates for agriculture due to the adjustable spectrum and excellent match with the dyes of the plants. Here, Mn4+ activated double perovskite LaMg 2/3 Mn 1/3 O 3 NIR phosphor was prepared, which showed high match with the absorption spectra of phytochrome P FR. Concentration of Mg2+ was decreased to create octahedron vacancy into the lattice to improve the luminescence performance. Structure and luminescence variation of the phosphors LaMg 2/3- x Nb 1/3 O 3 :Mn4+ along with the increased vacancy concentration were carried out. Relative sensitivity S r of the optimized LaMg 0.57 Nb 0.33 O 3 :Mn4+ reaches 3.109 % and exhibits great application potential in temperature sensor. Both the NIR light emitting diode (LED) and white LED were fabricated by using this double perovskite NIR phosphor and applied to the cultivation of dragon fruit as light compensation. The dragon fruits matured faster than those without any light compensation. These results confirm the great application potential of this double perovskite NIR phosphor in temperature sensor and plant cultivation. [ABSTRACT FROM AUTHOR]

This study aimed to determine the biofunctional properties, such as antioxidant, antimicrobial, anticancer, and antidiabetic activities, of peel extracts obtained through microwave‐assisted extraction (MAE) of red beet, dragon fruit, and prickly pear peels using water as a green solvent. Results indicated that the peel extracts exhibited high total phenolic content (TPC), ranging from 345.93 to 1651.17 mg GAE/L. The DPPH scavenging capacity of the extracts ranged between 7.16 and 34.65 mg/mL, while the ABTS scavenging capacity ranged between 0.93 and 15.27 mg/mL. Dragon fruit peel extract (DFE) and prickly pear peel extract (PPE) showed significant α‐glucosidase inhibition effects, with 67.79% and 37.50% inhibitions, respectively. Moreover, significant antibacterial activities were observed against five pathogenic bacterial strains (B. cereus, E. coli, S. aureus, L. monocytogenes, and S. enterica) at various concentrations of extracts. The cytotoxic effect of the extracts on HT‐29 and HeLa cancer cells was also observed. The most abundant phenolic compound in DFE was rutin (0.558 mg/g); in PPE, hesperidin was the most abundant (0.596 mg/g); and in red beet peel extract (RBE), hesperidin (0.426 mg/g) was the predominant compound. [ABSTRACT FROM AUTHOR]

In our study, the biological activity of Hylocereus polyrhizus obtained from Türkiye was determined. Firstly, antimicrobial activity of methanol extracts obtained from H. polyrhizus against test microorganisms was determined by disc diffusion susceptibility assay, minimum inhibition concentration (MIC) and minimum fungicidal or bactericidal concentration (MFC or MBC) tests. Then, viable cell counting against Limosilactobacillus fermentum MA-7 using the macro-dilution method was used to determine the probiotic-promoting effect of the fruit extracts. Finally, the photo-protective activity of the extracts and extract-cream mixtures was obtained spectrophotometrically. The inhibition zone diameter of the peel extract against the test microorganisms is in the range of 9.60-12.37 mm and the fruit extract is in the range of 7.85-12.00 mm. MBC values ranged from 40 µg/µl to 80 µg/µl in peel extract and between 10 µg/µl and >80 µg/µl in the fruit extract. The inhibition zone diameters of the extracts against probiotics were determined as 6.10 -10.25 mm. As a result of the viable cell count, the viability rate of L. fermentum MA-7 increased as the fruit methanol extract concentration and time increased. The photoprotective activities of the peel and fruit extracts were determined as 13.17 and 12.26. It was determined that the extracts increased sun protection factor (SPF) value of the cream. The results indicated that H. polyrhizus extracts may have potential for use in the nutraceutical products, cosmetic and pharmaceutical industries. [ABSTRACT FROM AUTHOR]

Anthracnose is one of the destructive diseases of pitaya that seriously affects the plant growth and fruit quality and causes significant yield and economic losses worldwide. However, information regarding the species of pathogens that cause anthracnose in pitaya (Hylocereus undatus) fruits in Gansu Province, China, and its pathogenic mechanism is unknown. Thus, the purposes of our present study were to identify the species of pathogens causing H. undatus fruits anthracnose based on the morphological and molecular characteristics and determine its pathogenic mechanism by physiological and biochemical methods. In our present study, forty-six isolates were isolated from the collected samples of diseased H. undatus fruits and classified as three types (named as H-1, H-2, and H-3), according to the colony and conidium morphological characteristics. The isolation frequencies of H-1, H-2, and H-3 types were 63.04%, 21.74%, and 15.22%, respectively. The representative single-spore isolate of HLGTJ-1 in H-1 type has significant pathogenicity, and finally we identified Colletotrichum truncatum as the pathogen based on the morphological characteristics as well as multi-locus sequence analysis. Moreover, the H. undatus fruits inoculated with C. truncatum had a significantly increased activity of cell wall-degrading enzymes (CWDEs) cellulase (Cx), β-glucosidase (β-Glu), polygalacturonase (PG), and pectin methylgalacturonase (PMG), while having a decreased level of cell wall components of original pectin and cellulose in comparison to control. The average increased activities of Cx, β-Glu, PG, and PMG were 30.73%, 40.40%, 51.55%, and 32.23% from day 0 to 6 after inoculation, respectively. In contrast, the average decreased contents of original pectin and cellulose were 1.82% and 16.47%, respectively, whereas the average increased soluble pectin content was 38.31% in comparison to control. Our results indicate that C. truncatum infection increased the activities of CWDEs in H. undatus fruits to disassemble their cell wall components, finally leading to the fruits' decay and deterioration. Thus, our findings will provide significant evidence in the controlling of pitaya anthracnose in the future. [ABSTRACT FROM AUTHOR]

The present work intended to explore the synergistic effects of incorporating carbon nanotubes infused with magnesium oxide nanofluid and dragon fruit extract as a natural additive in photovoltaic–thermal collector systems on its performance in terms of both electrical and thermal energy yield. The proposed methodology was meticulously developed and implemented to assess the efficiency of a hybrid system integrating nano-phase change material and nanofluid in a photovoltaic–thermal configuration. This novel setup was subjected to a comprehensive comparative analysis against a traditional liquid-cooled photovoltaic–thermal system and a standalone photovoltaic module. The study also encompassed variations in cooling techniques, including water with different flow rates for the photovoltaic–thermal system, and the incorporation of nano-phase change material and nanofluid with varying concentrations and flow rates within the hybrid collector. The outcomes demonstrated that at these respective flow rates, the maximum thermal efficiency of the photovoltaic–thermal collector stood at 66.28% and 74.02%. Likewise, the peak electrical efficiency at the same flow rates was recorded as 20.79% and 21.96%. The outcomes of the investigation highlight the higher thermal conductivity of the nanofluid compared to the base fluid, leading to a slight augmentation in fluid density and viscosity. The results obtained from this investigation were found to be consistent with those documented in existing scientific literature. [ABSTRACT FROM AUTHOR]

The aim of this work was to determine the impact of pitahaya peel flour (PPF) addition, at different concentrations (1.5 and 3%) on the chemical, physico-chemical and sensorial properties of beef burgers. This assessment was based on: proximal analysis, physico-chemical and cooking properties, lipid oxidation and sensory assay of beef burgers. The burger reformulation with pitahaya peel flour had no effect (p > 0.05) on the chemical composition of raw burgers, whilst the cooked burger increased (p < 0.05) the protein and fat content and in the same way increased the calcium, potassium and phosphorus content (p < 0.05) with respect to the control sample. The colour coordinates redness (increased) and yellowness (decreased) were affected (p < 0.05) by the pitahaya peel flour addition. From a textural point of view, all parameters, except springiness, were affected (p < 0.05) by the incorporation of pitahaya peel flour. In addition, the use of pitahaya peel flour reduces (p < 0.05) the cooking loss and the shrinkage of burgers. The raw and cooked beef burger added with pitahaya peel flour showed lower lipid oxidation values (p < 0.05) than those of the control sample. The hedonic scores for all burgers analysed ranged from 6 to 7, which indicates a high degree of acceptance by the panellists. The results obtained indicated that pitahaya peel flour could be used as a novel ingredient in the development of beef burgers. [ABSTRACT FROM AUTHOR]

Owing to the increasing worldwide population explosion, managing waste generated from the food sector has become a cross-cutting issue globally, leading to environmental, economic, and social issues. Circular economy-inspired waste valorization approaches have been increasing steadily, generating new business opportunities developing valuable bioproducts using food waste, especially fruit wastes, that may have several applications in energy–food–pharma sectors. Dragon fruit waste is one such waste resource, which is rich in several value-added chemicals and oils, and can be a renewable resource to produce several value-added compounds of potential applications in different industries. Pretreatment and extraction processes in biorefineries are important strategies for recovering value-added biomolecules. There are different methods of valorization, including green extractions and biological conversion approaches. However, microbe-based conversion is one of the advanced technologies for valorizing dragon fruit waste into bioethanol, bioactive products, pharmaceuticals, and other valued products by reusing or recycling them. This state-of-the-art review briefly overviews the dragon fruit waste management strategies and advanced eco-friendly and cost-effective valorization technologies. Furthermore, various applications of different valuable bioactive components obtained from dragon fruit waste have been critically discussed concerning various industrial sectors. Several industrial sectors, such as food, pharmaceuticals, and biofuels, have been critically reviewed in detail. [ABSTRACT FROM AUTHOR]

The pitaya is a common fruit in southern China, but the growing environment of pitayas is complex, with a high density of foliage. This intricate natural environment is a significant contributing factor to misidentification and omission in the detection of the growing state of pitayas. In this paper, the growth states of pitayas are classified into three categories: flowering, immature, and mature. In order to reduce the misidentification and omission in the recognition process, we propose a detection model based on an improvement of the network structure of YOLOv8, namely YOLOv8n-CBN. The YOLOv8n-CBN model is based on the YOLOv8n network structure, with the incorporation of a CBAM attention mechanism module, a bidirectional feature pyramid network (BiFPN), and a C2PFN integration. Additionally, the C2F module has been replaced by a C2F_DCN module containing a deformable convolution (DCNv2). The experimental results demonstrate that YOLOv8n-CBN has enhanced the precision, recall, and mean average precision of the YOLOv8n model with an IoU threshold of 0.5. The model demonstrates a 91.1% accuracy, a 3.1% improvement over the original model, and an F1 score of 87.6%, a 3.4% enhancement over the original model. In comparison to YOLOv3-tiny, YOLOv5s, and YOLOv5m, which are highly effective target detection models, the mAP@0.50–0.95 of our proposed YOLOv8n-CBN is observed to be 10.1%, 5.0%, and 1.6% higher, respectively. This demonstrates that YOLOv8n-CBN is capable of more accurately identifying and detecting the growth status of pitaya in a natural environment. [ABSTRACT FROM AUTHOR]

In this study, dragon fruit peels were subjected to different times (5, 10, 15, 20 and 25 min) of ultrasound pretreatment (4.870 W m−2/25°C) and then freeze‐drying (FD) (−56°C/48 h) to obtain powder particles, which underwent physical–chemical, functional and structural characterisation. Additionally, the extraction of total phenolic compound (TPC) was evaluated through ultrasound‐assisted kinetics and mechanical agitation. Finally, films based on black rice starch were developed with the addition (0.5, 1.5 and 2.5%) of dragon fruit peel powder particles, and their physical, barrier, antioxidant, structural and mechanical properties were evaluated. The results obtained showed that all particles had low water content (4.21%–6.58%) and high solubility (84.62%–88.73%) with a reduction in particle size (23.21–39.32 μm). In the extraction of TPC, the synergistic effect of the tested conditions increased efficiency, reaching 686.66 mg GAE/100 g for pretreated dragon fruit peel powder particles (25 min) in 180 min of extraction. Furthermore, the type 'A' crystallinity structure did not change significantly with pretreatment, but there was a reduction in peak area, reflecting on relative crystallinity, with a maximum decrease of 29.04%. The starch‐based films produced with 2.5% dragon fruit peel powder particles showed low solubility (28.72%) and low water vapour permeability (5.03 × 10−8 g mm s−1 m2 Pa), however, they contained higher levels of TPC (31.78 mg GAE/100 g film) and antioxidant activity (8.17 μmol TE/g film) that were easily migrated in aqueous medium. Furthermore, they also presented low values of tensile strength at break (3.41 MPa) and Young's modulus (4.18 MPa), as well as a high value of elongation at break (45.82%). Finally, our results highlight the potential of ultrasound as a pretreatment for FD of dragon fruit peels, paving the way for the development of rice starch‐based food packaging. [ABSTRACT FROM AUTHOR]

Red dragon fruit (Hylocereus polyrhizus), recognized globally for its substantial nutrient content and health benefits, has been extensively studied; studies have particularly focused on the fruit, while the composition of the stem remains less explored. This research focuses on optimizing fermentation parameters for red dragon fruit wine, specifically examining yeast-strain selection, juice-to-water dilution ratios, and yeast concentrations. Saccharomyces cerevisiae RV002 emerged as the optimal strain due to its robust performance and adaptability under adverse conditions. The study identified a 50% dilution ratio as ideal for maximizing clarity and the sensory attributes of the wine, whereas dilution ratios exceeding 90% significantly reduced ethanol content below acceptable commercial standards. An optimal yeast concentration of 1 g/L was found to balance microbial suppression and alcohol yield effectively; deviations from this concentration led to microbial contamination or impaired fermentation dynamics. Fermentation markedly altered the biochemical properties of Hylocereus polyrhizus, reducing sugar and vitamin C levels while increasing polyphenol content and antioxidant activity, thereby enhancing potential health benefits. These findings underscore the transformative effects of microbial activity on the substrate's chemical landscape and highlight the potential of tailored fermentation strategies to enhance the utility and value of underutilized fruits in sustainable agricultural practices. [ABSTRACT FROM AUTHOR]

Abstract\nNOVELTY STATEMENTThe peel of Hylocereus undatus was employed in the preparation of biochar and firstly applied for tetracycline removal from aqueous solution. Based on different characterization techniques, the material was found to possess a variety of surface functional groups on a porous structure and a pH point of zero charge (pHpzc) of 9.3. Adsorption of tetracycline (TC) was conducted under varying conditions, revealing significant effects of carbonization temperature, solution pH, adsorbent dose, ionic strength, contact time and initial concentration of TC on the biochar adsorption capacity. Kinetic data on TC adsorption were best described using the Elovich kinetic model, with an initial adsorption rate of 167.3 mg g−1 min−1. Isotherm data on adsorption of the desired biochar showed the best fit with the Temkin isotherm model, followed by the Langmuir model, displaying maximum adsorption capacity at 12.4 mg g−1. The electrostatic interactions between the charged biochar surfaces and certain fractions of TC were proposed as the major mechanism, together with H-bonding, pore-filling effect and π–π interaction. This study demonstrates great potential of H. undatus peel as a starting material to prepare an effective and reusable adsorbent in the removal of TC.Literature is available on a large number of plant-based biochar adsorbents for the removal of antibiotics. However, to the best of our knowledge, no report has been published on applying biochar derived from Hylocereus undatus peel for antibiotics removal. This type of fruit peel is available in massive amounts in Vietnam and is considered as an agricultural solid waste. Therefore, to fill the gap in the literature and to converse this leftover waste into a value-added byproduct, we chose this study to prepare H. undatus peel-derived biochar for the elimination of TC in an aqueous solution. [ABSTRACT FROM AUTHOR]

Dragon fruit stem disease significantly affects both the quality and yield of dragon fruit. Therefore, there is an urgent need for an efficient, high-precision intelligent detection method to address the challenge of disease detection. To address the limitations of traditional methods, including slow detection and weak micro-integration capability, this paper proposes an improved YOLOv8-G algorithm. The algorithm reduces computational redundancy by introducing the C2f-Faster module. The loss function was modified to the structured intersection over union (SIoU), and the coordinate attention (CA) and content-aware reorganization feature extraction (CARAFE) modules were incorporated. These enhancements increased the model's stability and improved its accuracy in recognizing small targets. Experimental results showed that the YOLOv8-G algorithm achieved a mean average precision (mAP) of 83.1% and mAP50:95 of 48.3%, representing improvements of 3.3% and 2.3%, respectively, compared to the original model. The model size and floating point operations per second (FLOPS) were reduced to 4.9 MB and 6.9 G, respectively, indicating reductions of 20% and 14.8%. The improved model achieves higher accuracy in disease detection while maintaining a lighter weight, serving as a valuable reference for researchers in the field of dragon fruit stem disease detection. [ABSTRACT FROM AUTHOR]

Dragon fruits have abundant nutritional and antioxidant properties. High temperatures limit the growth and production of dragon fruits. Hemin can effectively alleviate abiotic stress in plants. However, the regulatory effect of Hemin on dragon fruit under heat stress remains unclear. In this study, we explored the impacts of foliar application of Hemin on dragon fruit size, yield and quality during the high temperatures of the summer season. In this experiment, dragon fruit variety 'Jindu No. 1' was used as material and treated with three Hemin concentrations, i.e., H1: 1 μmol.L−1, H2: 10 μmol.L−1, H3: 100 μmol.L−1, compared with CK: control. The results show that exogenous Hemin increased the single fruit weight, yield, fruit shape index and edible rate. It also improved pericarp L* value, a* value, C* and decreased ho, improving the peel colour; exogenous Hemin enhanced soluble solids content and phenolic compounds content and antioxidant enzyme activities in the pulp of dragon fruit. In addition, exogenous Hemin increased the content of chlorophyll content in dragon fruit stems. Differential metabolites determined by metabolomic assay also indicated that Hemin significantly increased the content of active substances such as selagin. Additionally, the Hemin treatment H2 also activated the activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), which helps to mitigate the effects of high temperatures on dragon fruit. The current findings strongly advocate that H2 treatment may effectively counteract the adverse effects of heat stress by regulating the morph-physiological and antioxidant traits. [ABSTRACT FROM AUTHOR]

Pitaya, Hylocereus spp. (Caryophyllales: Cactaceae) has been one of the new species cultivated in Türkiye in recent years. There are many limiting factors, including pests, diseases and weeds, to decrease the yield and fruit quality in pitaya (dragon fruit) areas in Türkiye. In addition, the pests of pitaya is not fully studied therefore this research has been conducted to determine mealybug species in pitaya greenhouses in Türkiye. The present study aimed to determine mealybug species and its natural enemies (parasitoids, and predator insects) in pitaya greenhouses in Adana, Mersin, and Antalya between 2021 and 2022. According to results of this study, 2 different mealybug species was determined; Phenacoccus solenopsis Tinsley 1898, Phenacoccus madeirensis Green, 1923 (Hemiptera: Pseudococcidae) in pitaya greenhouses. Moreover one parasitoid (Aenasius arizonensis Girault (Hymenoptera: Encyrtidae), and two different predatory insects from 2 different orders and 2 different families (Chrysoperla carnea Stephens (Neuroptera: Chrysopidae), Nephus includens Kirsch (Coleoptera: Coccinellidae) were detected within this research. These two invasive mealybug species have wide spectrum host plants and may cause economically important damages to pitaya, if natural enemies cannot work successfully or control strategies do not applied properly in pitaya greenhouses. [ABSTRACT FROM AUTHOR]

Pitaya fruit is a significant agricultural commodity in southern China. The traditional method of determining the ripeness of pitaya by humans is inefficient, it is therefore of the utmost importance to utilize precision agriculture and smart farming technologies in order to accurately identify the ripeness of pitaya fruit. In order to achieve rapid recognition of pitaya targets in natural environments, we focus on pitaya maturity as the research object. During the growth process, pitaya undergoes changes in its shape and color, with each stage exhibiting significant characteristics. Therefore, we divided the pitaya into four stages according to different maturity levels, namely Bud, Immature, Semi-mature and Mature, and we have designed a lightweight detection and classification network for recognizing the maturity of pitaya fruit based on the YOLOv8n algorithm, namely GSE-YOLO (GhostConv SPPELAN-EMA-YOLO). The specific methods include replacing the convolutional layer of the backbone network in the YOLOv8n model, incorporating attention mechanisms, modifying the loss function, and implementing data augmentation. Our improved YOLOv8n model achieved a detection and recognition accuracy of 85.2%, a recall rate of 87.3%, an F1 score of 86.23, and an mAP50 of 90.9%, addressing the issue of false or missed detection of pitaya ripeness in intricate environments. The experimental results demonstrate that our enhanced YOLOv8n model has attained a commendable level of accuracy in discerning pitaya ripeness, which has a positive impact on the advancement of precision agriculture and smart farming technologies. [ABSTRACT FROM AUTHOR]

Under changing climatic conditions, plants are simultaneously facing conflicting stresses in nature. Plants can sense different stresses, induce systematic ROS signals, and regulate transcriptomic, hormonal, and stomatal responses. We performed transcriptome analysis to reveal the integrative stress response regulatory mechanism underlying heavy metal stress alone or in combination with heat and drought conditions in pitaya (dragon fruit). A total of 70 genes were identified from 31,130 transcripts with conserved differential expression. Furthermore, weighted gene co‐expression network analysis (WGCNA) identified trait‐associated modules. By integrating information from three modules and protein–protein interaction (PPI) networks, we identified 10 interconnected genes associated with the multifaceted defense mechanism employed by pitaya against co‐occurring stresses. To further confirm the reliability of the results, we performed a comparative analysis of 350 genes identified by three trait modules and 70 conserved genes exhibiting their dynamic expression under all treatments. Differential expression pattern of genes and comparative analysis, have proven instrumental in identifying ten putative structural genes. These ten genes were annotated as PLAT/LH2, CAT, MLP, HSP, PB1, PLA, NAC, HMA, and CER1 transcription factors involved in antioxidant activity, defense response, MAPK signaling, detoxification of metals and regulating the crosstalk between the complex pathways. Predictive analysis of putative candidate genes, potentially governing single, double, and multifactorial stress response, by several signaling systems and molecular patterns. These findings represent a valuable resource for pitaya breeding programs, offering the potential to develop resilient "super pitaya" plants. [ABSTRACT FROM AUTHOR]

Dextransucrases play a crucial role in the production of dextran from economical sucrose; therefore, there is a pressing demand to explore novel dextransucrases with better performance. This study characterized a dextransucrase enzyme, LmDexA, which was identified from the Leuconostoc mesenteroides NN710. This bacterium was isolated from the soil of growing dragon fruit in Guangxi province, China. We successfully constructed six different N-terminal truncated variants through sequential analysis. Additionally, a truncated variant, ΔN190LmDexA, was constructed by removing the 190 amino acids fragment from the N-terminal. This truncated variant was then successfully expressed heterologously in Escherichia coli and purified. The purified ΔN190LmDexA demonstrated optimal hydrolysis activity at a pH of 5.6 and a temperature of 30 °C. Its maximum specific activity was measured to be 126.13 U/mg, with a Km of 13.7 mM. Results demonstrated a significant improvement in the heterologous expression level and total enzyme activity of ΔN190LmDexA. ΔN190LmDexA exhibited both hydrolytic and transsaccharolytic enzymatic activities. When sucrose was used as the substrate, it primarily produced high-molecular-weight dextran (>400 kDa). However, upon the addition of maltose as a receptor, it resulted in the production of a significant amount of oligosaccharides. Our results can provide valuable information for enhancing the characteristics of recombinant dextransucrase and potentially converting sucrose into high-value-added dextran and oligosaccharides. [ABSTRACT FROM AUTHOR]

Cavendish banana and red dragon fruit contain vitamins, fiber, and antioxidants. However, the fruits are quickly spoiled, and requires processing into instant powder with the foam mat drying for extend the storage time. Okara protein hydrolysate through enzymatic hydrolysis process and soy protein isolate will be used as foaming agent. This study evaluated the effect of adding okara protein hydrolysate and soy protein isolate on the physicochemical properties such as water absorption index (WAI), water solubility index (WSI), hygroscopicity, bulk density, color, viscosity, antioxidant activity, vitamin C, total soluble solid, titratable acidity, proximate composition, acceptance test, and shelf life of instant powder. The results showed that the instant powder with okara protein hydrolysate produced a moisture content of 2.81%, WAI 0.69 g/g, and bulk density 0.39 g/cm3, lower than the addition of soy protein isolate. The acceptance test of instant powder with okara protein hydrolysate had an average score of 4.40 (neutral) to 5.06 (slightly like). The addition of okara hydrolysate can significantly (p < 0.05) increased the WSI value to 81.42%, antioxidant activity 49.86%, and longer shelf life of 144 days. Therefore, okara protein hydrolysate can be used as foaming agents with natural antioxidants and produced the stable instant powder. [ABSTRACT FROM AUTHOR]

Dragon fruit has significant economic value in many countries due to has excellent nutritional content, health advantages, and adaptability to different climates, making it an important crop in the global fruit industry. This study aimed to gather comprehensive nutritional data on three dragon fruit cultivars by analysing the levels of micronutrients, fibre, carbohydrates, antioxidants, vitamins, and minerals in their pulps. Uniform dragon fruit samples underwent thorough analysis for proximate composition, mineral content, pigments, antioxidants, and vitamin C, with statistical methods used to assess significant differences among the parameters studied. The proximate composition analysis revealed significant differences among the three dragon fruit cultivars. Among the proximate components, protein (0.40 ± 0.02 g/100 g), moisture (91.33 ± 0.88%), crude fibre (0.32 ± 0.07 g/100 g), and ash (1.27 ± 0.09 g/100 g) were more abundant in Hylocereus costaricensis than in Hylocereus undatus and Hylocereus megalanthus. On the other hand, Hylocereus undatus had higher carbohydrate (17.02 ± 0.63 g/100 g) and energy (69.74 ± 2.44 kcal/100 g) contents. K (7.23 ± 0.35 mg/100 g), Ca (1.61 ± 0.13 mg/100 g), Fe (1.84 ± 0.05 mg/100 g), and Zn (0.37 ± 0.034 mg/100 g) are highly abundant in H. costaricensis. Additionally, Hylocereus costaricensis had the highest anthocyanin content (120.15 ± 3.29 mg/g FW) and total carotenoid content (72.51 ± 1.62 mg/g FW), along with the highest vitamin C content (8.92 ± 0.13 mg/g FW) and total soluble phenolic content (572.48 ± 20.77 mg/100 g). Its remarkable antioxidant activity was further highlighted by the lowest SC50 value (13.50 ± 0.4 mg/mL) for its DPPH radical scavenging capacity. The total soluble sugar content was highest in Hylocereus megalanthus (8.72 ± 0.30 g/100 g FW). Hierarchical clustering analysis revealed distinct trait and genotype associations; among the studied cultivars, Hylocereus costaricensis demonstrated superior performance across multiple traits. Correlation analysis indicated significant positive correlations among several traits, while principal component analysis highlighted the contribution of each trait to overall variance, with PC1 explaining 73.95% of the total variance. This study highlights the nutritional variations among dragon fruit cultivars, with Hylocereus costaricensis showing superior performance, guiding dietary planning and functional food development. [ABSTRACT FROM AUTHOR]

The objective of this study was to investigate the effect of microencapsulated bioactive compounds from lemongrass mixed dragon fruit peel pellet (MiEn-LEDRAGON) supplementation on fermentation characteristics, nutrient degradability, methane production, and the microbial diversity using in vitro gas production technique. The study was carried out using a completely randomized design (CRD) with five levels of MiEn-LEDRAGON supplementation at 0, 1, 2, 3, and 4% of the total dry matter (DM) substrate. Supplementation of MiEn-LEDRAGON in the diet at levels of 3 or 4% DM resulted in increased (p < 0.05) cumulative gas production at 96 hours (h) of incubation time, reaching up to 84.842 ml/ 0.5 g DM. Furthermore, supplementation with 3% MiEn-LEDRAGON resulted in higher in vitro nutrient degradability and ammonia–nitrogen concentration at 24 h of the incubation time when compared to the control group (without supplementation) by 5.401% and 11.268%, respectively (p < 0.05). Additionally, supplementation with MiEn-LEDRAGON in the diet led to an increase in the population of Fibrobacter succinogenes at 24 h and Butyrivibrio fibrisolvens at 12 h, while decreasing the population of Ruminococcus albus, Ruminococcus flavefaciens, and Methanobacteriales (p < 0.05). Moreover, supplementation of MiEn-LEDRAGON in the diet at levels of 2 to 4% DM resulted in a higher total volatile fatty acids (VFA) at 24 h, reaching up to 73.021 mmol/L (p < 0.05). Additionally, there was an increased proportion of propionic acid (C3) and butyric acid (C4) at 12 h (p < 0.05). Simultaneously, there was a decrease in the proportion of acetic acid (C2) and the ratio of acetic acid to propionic acid (C2:C3), along with a reduction of methane (CH4) production by 11.694% when comparing to the 0% and 3% MiEn-LEDRAGON supplementation (p < 0.05). In conclusion, this study suggests that supplementing MiEn-LEDRAGON at 3% of total DM substrate could be used as a feed additive rich in phytonutrients for ruminants. [ABSTRACT FROM AUTHOR]

The usage of natural food colorants in food processing is increasing nowadays. Red dragon fruit peel is rich in betacyanin pigment. The peel, which is generally categorized as food waste, can be extracted and used as a natural food colorant that produces a red-violet color. Pigment extraction using ohmic heating can shorten the extraction time. Ohmic heating has a very fast heating rate and can avoid excessive thermal damage to pigments. This study aims to determine the effect of solvent type and extraction temperature on chemical and physical characteristics, as well as process parameters (heating time, electrical conductivity, and ohmic heating efficiency) of extracts that have been extracted using the ohmic heating method. The study used a 2-factor group randomized design, namely solvent type (distilled water, citric acid 0.2%, and NaCl 0.2%) and extraction temperature (50, 60, 70, 80, and 90°). The results showed that the interaction between solvent type and extraction temperature significantly affected betacyanin content. Each factor (solvent type and extraction temperature) had a significant effect on the heating time, electrical conductivity, and extraction yield. NaCl 0.2% solvent can increase electrical conductivity so that the heating time is faster and pigments can be extracted from the cells. Ohmic heating can be used for the extraction process of betacyanin pigment from red dragon fruit peel, which is heat-labile. [ABSTRACT FROM AUTHOR]

The oriental fruit fly Bactrocera dorsalis (Hendel) has been reported on the white fleshed dragon fruit Selenicereus undatus (Haworth) D.R.Hunt for the first time in India. Symptoms of infestation and the duration of all life stages were observed. Females laid eggs just beneath the mature fruit skin. The tissue surrounding the egg mass turned light yellow. Oviposition punctures were visible on the fruit rind and the feeding of the maggots led to decay of internal contents, foul smell and the fruit turned in to a discolored semi liquid mass. Full grown maggots exited the fruit and entered a period of inactivity before pupation in moist soil. The egg, maggot and pupal stages lasted for 1.6± 0.40, 8.0 ± 0.40 and 8.0 ± 0.31 days, respectively under laboratory conditions. Adult longevity was 9.6 ± 0.5 days in the case of females and 6.4 ± 0.50 days for males. [ABSTRACT FROM AUTHOR]

In order to effectively solve the problems of the complex growth state of dragon fruit and how the picking process is mostly manual, this study designed a picking and selecting integrated remote-operation-type dragon-fruit-picking device. Based on SOLIDWORKS 2020 software for the three-dimensional digital design and overall assembly of key components, the structure and working theory of the machine are introduced. By improving the high-recognition-rate dragon fruit target detection algorithm based on YOLOv5, better recognition and locating effects were achieved for targets with a small size and high density, as well as those in bright-light scenes. Serial communication, information acquisition, and the precise control of each picking action were realized by building the software and hardware platforms of the picking device control system. By analyzing the working principle of the mechanical system and the mechanics of the machine picking process, the critical factors affecting the net picking rate and damage rate of the dragon-fruit-picking device were confirmed. Based on the force and parameter analysis of the test results, it was confirmed that the machine had an optimal picking influence when the flexible claw closing speed was 0.029 m/s, the electric cylinder extending speed was 0.085 m/s, and the mechanical arm moving speed was 0.15 m/s. The net picking rate of the device reached 90.5%, and the damage rate reached 2.9%. The picking device can complete the picking of a single dragon fruit, as well as a plurality of fruits grown at a growing point, and integrates the integration of picking fruits, removing bad fruits, and sorting fruits, which can improve the efficiency of dragon fruit harvesting and replace manual work. [ABSTRACT FROM AUTHOR]

Red dragon fruit is gaining popularity globally due to its nutritional value and bioactive components. The study aimed to assess the phytochemical, nutritional composition, antioxidant, antibacterial, and cytotoxic properties of extracts from the South Chinese red dragon fruit peel, flesh, and seeds. Extract fractions with increasing polarity (ethyl acetate50 values ranging from 3.25–8.30 μg/mL. The flesh MT and ET extract fractions demonstrated substantial inhibitory effects, with MIC50 ranging 73.62–98.85 μg/mL, against several multidrug-resistant bacteria. Cytotoxicity assays revealed no adverse effects of the crude extract fractions on human keratinocytes (HaCaT) cells (from adult human skin). Liquid chromatography with tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) analyses unveiled a wide array of compounds with known and unknown antibacterial and antioxidant activities. Thereafter, pharmacological property screening and molecular docking were performed. The results identified four active compounds, baicalein, quercetin, morin, and isorhamnetin, which demonstrated strong binding affinity with the selected target proteins and complied with most of the pharmacological parameters. In conclusion, this study provides essential information on the key nutrients present in the peel, flesh, and seeds of the red dragon fruit from Southern-China. Furthermore, it highlights the potential of ET and MT fractions, which contain compounds with promising antibacterial properties. [ABSTRACT FROM AUTHOR]

The synthesis of betalain using microorganisms is an innovative developmental technology, and the excavation of microorganisms closely related to betalain can provide certain theoretical and technical support to this technology. In this study, the characteristics of soil microbial community structures and their functions in the rhizospheres of white-fleshed dragon fruit (Hylocereus undatus) and red-fleshed dragon fruit (Hylocereus polyrhizus) were analyzed. The results show that the soil bacterial and fungal compositions in the rhizospheres were shaped differently between H. undatus and H. polyrhizus. Bacterial genera such as Kribbella and TM7a were the unique dominant soil bacterial genera in the rhizospheres of H. undatus, whereas Bradyrhizobium was the unique dominant soil bacterial genus in the rhizospheres of H. polyrhizus. Additionally, Myrothecium was the unique dominant soil fungal genus in the rhizospheres of H. polyrhizus, whereas Apiotrichum and Arachniotus were the unique dominant soil fungal genera in the rhizospheres of H. undatus. Moreover, TM7a, Novibacillus, Cupriavidus, Mesorhizobium, Trechispora, Madurella, Cercophora, and Polyschema were significantly enriched in the rhizospheres of H. undatus, whereas Penicillium, Blastobotrys, Phialemonium, Marasmius, and Pseudogymnoascus were significantly enriched in the rhizospheres of H. polyrhizus. Furthermore, the relative abundances of Ascomycota and Penicillium were significantly higher in the rhizospheres of H. polyrhizus than in those of H. undatus. [ABSTRACT FROM AUTHOR]

This study investigated the effect of exogenous plant growth regulators (PGR), namely jasmonic acid (JA) and methyl jasmonate (MeJA), on the physicochemical properties of flesh and peels of red-fleshed dragon fruit (Hylocereus polyrhizus). The fruit was sprayed with 100 and 1,000 ppm of JA and MeJA at 15 and 22 days of anthesis and harvested after 35 days. Then, the flesh and peels were analyzed for total soluble solids (TSS), total betacyanins, betanin, total phenolics (TP), total flavonoids (TF), and color characteristics. The fruit peels contained significantly higher (p<0.05) TP and antioxidant activities compared to flesh. No significant difference was detected between the variables in the peels, except for significantly higher (p<0.05) of total betacyanins (~295.6 and ~299.9 mg/100 g) and TP (~614.1 and 566.1 mg GAE/100 g) were recorded in control and 100 ppm MeJA, respectively. In the flesh, 1,000 ppm MeJA-treated fruit possessed the highest total betacyanins (~139.2 mg/100 g), betanin (~356.0 mg/g), TP (~244.9 mg GAE/100 g), TF (~329.0 mg CE/100 g), Trolox equivalent antioxidant capacity (TEAC) (63.2 µmol TE/g) and reducing power (~21.5 µmol TE/g). Overall, 1,000 ppm MeJA was more effective in enhancing the accumulation of bioactive compounds and antioxidant activities in the flesh of red-fleshed dragon fruit compared to other PGR treatments. [ABSTRACT FROM AUTHOR]

Betacyanin can be found in the peel or pulp of dragon fruit. As a natural pigment, betacyanin is unstable, so it requires encapsulation technology to maintain its quality. The stability of encapsulated betacyanin from dragon fruit peel compared to dragon fruit pulp has yet to be discovered. This study aims to compare the stability of encapsulated betacyanin (with maltodextrin and gum Arabic) from dragon fruit peel and pulp dried with vacuum drying. Dragon fruit peel extraction utilized a 50% aqueous ethanol solvent, while pulp juice extraction was performed. The ratio of dragon fruit extract to coating materials was set at 1:3 (solid/solid). Research shows that dragon fruit juice powder had higher stability and phytochemical concentrations than the ethanol extract of dragon fruit peel powder during 30 days of storage. Despite similar color stability (similar range value of ΔE), the color from dragon fruit juice powder more closely resembled the natural fruit, albeit with weaker antioxidant activity than the peel powder. Betacyanin concentration in juice powder was notably higher (82.56–156.82 μg/g) than in the ethanol extract of dragon fruit peel powder (52.51–75.12 μg/g). A combination of maltodextrin and Arabic gum (1:1) as coating materials demonstrated the highest concentrations of total phenolic and total betacyanin (81.15–95.87 mg/g and 121.91–156.82 μg/g, respectively) during the storage period. These findings contribute to our comprehension of betacyanin stability and functionality, facilitating precise applications in industrial processing environments based on their source attributes. [ABSTRACT FROM AUTHOR]

To ascertain their potential applications in the food industry, dragon fruit varieties, namely H. undatus and H. polyrhizus, were thoroughly analyzed for their physical, nutritional, and phytochemical properties. The focus was on pulp and juice, emphasizing color, mineral content, proximate analysis, and phytochemical constituents. Red flesh dragon fruit displayed a bright pink color, a slightly smaller length (9.1 cm), and a larger diameter (8.3 cm) compared to white flesh dragon fruit (9.9 cm length, 7.53 cm diameter). Red flesh dragon fruit also exhibited higher circumference and weight. White flesh dragon fruit demonstrated superior juice yield (36.23 %) compared to red flesh dragon fruit (35.28 %). Red flesh dragon fruit had higher levels of total sugar (8.45 %), protein (1.36 %), and ascorbic acid (19.83 mg/100g) in its pulp. It also showed elevated mineral content of calcium, magnesium, and phosphorus. Conversely, white flesh dragon fruit had higher fat content (0.65 %) and carbohydrate content (9.76 %) in its pulp. White flesh dragon fruit displayed brighter color characteristics with higher L*, a*, and b* values. Phytochemical analysis revealed the presence of betacyanin in red flesh dragon fruit (30.87 mg/100g) but not in white flesh dragon fruit. Red flesh dragon fruit exhibited significantly higher total phenolic content in pulp (49.67 mg GA/100g) and juice (41.25 mg GA/100g) than white flesh dragon fruit. These findings highlight substantial differences (P < 0.05) between red and white flesh dragon fruit in physical, nutritional, and phytochemical aspects, offering valuable insights for their incorporation into diverse food products, such as beverages and ice cream. [ABSTRACT FROM AUTHOR]

The cultivation of dragon fruit (Hylocereus spp.) in the Asir region has gained momentum, prompting a shift towards sustainable agricultural techniques. This research delves into the intricate relationship between lighting systems and plant growth parameters, specifically, shoot length and the number of branches, spanning from September 2022 to April 2023. Through rigorous analysis of variance, significant treatment effects were discerned across different months. During the initial phase (September to December 2022), variations in lighting systems markedly influenced the number of branches. Notably, October emerged as a pivotal month, eliciting a substantial increase in branch development. However, the effects witnessed in November and December were relatively subdued, possibly attributed to nuanced environmental factors. The subsequent period (January to April 2023) focused on shoot length. January and February exhibited conspicuous impacts, shaping shoot growth significantly. March maintained a consistent influence, while April witnessed a decline in the observed effects. This study underscores the paramount importance of comprehending both seasonal fluctuations and lighting conditions in the quest to optimize plant growth. The findings offer pivotal insights into agricultural practices and indoor cultivation methodologies, serving as a cornerstone for future research endeavors in this domain. [ABSTRACT FROM AUTHOR]

Trypsin, a novel superoxide scavenger, significantly enhances the storage quality of Hylocereus undatus (H. undatus). To elucidate the preservation mechanism of trypsin on H. undatus, a widely targeted metabolomic analysis, and transcriptomics analysis were conducted. Firstly, a total of 453 metabolites were identified, with organic acids and their derivatives constituting the largest proportion (25%). Amino acids and their metabolites, prominent among organic acids, were further analyzed. Among them, 73 metabolites were associated with amino acids, and 37 exhibited significant differences. The most enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was arginine biosynthesis (map00220), with polyamine metabolites showing the most pronounced differences, particularly spermine (FC = 1.7594). Compared with the control group, 4-hydroxy-2-oxoglutaric acid was significantly upregulated (FC = 2.117) in the process of spermine biosynthesis. Furthermore, the results of Gene Ontology (GO) and KEGG enrichment analysis of the H. undatus transcriptome profile revealed that trypsin treatment led to 187 differentially expressed genes associated with arginine. Both GO and KEGG analyses exhibited significant enrichment in the spermine biosynthetic process (GO:0006597) (map:00220) within the arginine biosynthesis pathway. Moreover, most enzymes and metabolites within the spermine biosynthesis pathway in H. undatus were upregulated. The results of the PPI network highlighted that ADC, SPDS, and SAMDC, among others, were pivotal proteins involved in trypsin-regulated arginine metabolism and spermine synthesis. This study revealed that trypsin could significantly delay postharvest senescence of H. undatus at room temperature. This effect might be attributed to trypsin triggering the synthesis of 4-hydroxy-2-oxoglutaric acid in the fruit peel, thereby promoting the biosynthesis of spermine and other polyamines. [ABSTRACT FROM AUTHOR]

Background: Geographical factors affect the nutritional, therapeutic and commercial values of fruits. Dragon fruit (Hylocereus spp) is a popular fruit in Asia and a potential functional food with diverse pharmacological attributes. Although it is produced in various localities, the information related to the altitudinal variation of dragon fruit nutrients and active compounds is scarce. Hence, this study aimed to investigate the variations in metabolite profiles of H. polyrhizus (variety Jindu1) fruit pulps from three different altitudes of China, including Wangmo (WM, 650 m), Luodian (LD, 420 m), and Zhenning (ZN, 356 m). Jindu1 is the main cultivated pitaya variety in Guizhou province, China. Results: The LC-MS (liquid chromatography-mass spectroscopy)-based widely targeted metabolic profiling identified 645 metabolites, of which flavonoids (22.64%), lipids (13.80%), phenolic acids (12.40%), amino acids and derivatives (10.39%), alkaloids (8.84%), and organic acids (8.37%) were dominant. Multivariate analyses unveiled that the metabolite profiles of the fruit differed regarding the altitude. Fruits from WM (highest altitude) were prime in quality, with higher levels of flavonoids, alkaloids, nucleotides and derivatives, amino acids and derivatives, and vitamins. Fruits from LD and ZN had the highest relative content of phenolic acids and terpenoids, respectively. We identified 69 significantly differentially accumulated metabolites across the pulps of the fruits from the three locations. KEGG analysis revealed that flavone and flavonol biosynthesis and isoflavonoid biosynthesis were the most differentially regulated. It was noteworthy that most active flavonoid compounds exhibited an increasing accumulation pattern along with the increase in altitude. Vitexin and isovitexin were the major differentially accumulated flavonoids. Furthermore, we identified two potential metabolic biomarkers (vitexin and kaempferol 3-O-[2-O-β-D-galactose-6-O-a-L-rhamnose]-β-D-glucoside) to discriminate between dragon fruits from different geographical origins. Conclusion: Our findings provide insights into metabolic changes in dragon fruits grown at different altitudes. Furthermore, they show that growing pitaya at high altitudes can produce fruit with higher levels of bioactive compounds, particularly flavonoids. [ABSTRACT FROM AUTHOR]

Simple Summary: Dragon fruit peel polyphenolic extract is a potential ruminal modulator endorsed by consumers for its inherent safety and acceptability. This in vitro study demonstrated that dragon fruit peel polyphenolic extract increased the concentration of volatile fatty acids in the rumen, reduced gas production, and enhanced the rumen bacterial abundances. These findings suggest that dragon fruit peel polyphenol extract has the potential to improve ruminal fermentation of sika deer and provides a theoretical basis for dragon fruit peel polyphenol extract to be used as a functional feed additive for Sika deer. The aim of this study is to investigate the effect of dragon fruit peel polyphenolic extract (DFPE) on gas production, rumen fermentation, and bacterial communities in sika deer using an in vitro technique. Three treatments with different DFPE levels (DFPE0, base diet; DFPE5, base diet + 5 mg/g DFPE; DFPE10, base diet + 10 mg/g DFPE, respectively; n = 6) were implemented. The phenolic composition of DFPE, gas production (GP), ammonia nitrogen (NH3-N), volatile fatty acid (VFA), and bacteria communities was evaluated after 24 h of incubation. The results showed that GP and NH3-N were reduced by DFPE supplementation. Total VFA, isovaleric acid, and valeric acid were increased (p < 0.05) by the addition of DFPE. No changes (p > 0.05) were observed in pH, acetic acid, propionic acid, isobutyric acid, butyric acid, and the ratio of acetic acid to propionic acid. Additionally, the alpha indexes, including Sobs, Shannon, and Ace, were increased by DFPE supplementation. Moreover, at the phylum level, DFPE supplementation increased (p = 0.01) Bacteroidota but reduced (p < 0.01) Firmicutes. At the genus level, compared to DFPE0, the DFPE10 had increased relative abundances of Rikenellaceae_RC9_gut_group (p < 0.01), norank_f_Muribaculaceae (p = 0.01), Lachnospiraceae_NK3A20_group (p < 0.01), Christensenellaceae_R-7_group (p < 0.01), and NK4A214_group (p < 0.01), decreased relative abundances of Streptococcus (p < 0.01), Oribacterium (p = 0.01), and Enterococcus (p < 0.01). Compared to DFPE0, DFPE5 had no change (p > 0.05) in all bacteria at the genus level except for decreased relative abundance of Enterococcus (p < 0.01). These results indicated that DFPE may be able to be used as a feed additive to enhance fermentation parameters and improve ruminal bacteria communities in Sika deer. [ABSTRACT FROM AUTHOR]

Dragon fruit peel, often discarded, is a valuable source of commercial pectin. This study investigates different extraction methods, including cold-water (CW), hot-water (HW), ultrasound (US), and novel enzyme extraction (xylanase: EZX), to extract pectins from dragon fruit peel and compare their characteristics. The pectin yield ranged from 10.93% to 20.22%, with significant variations in physicochemical properties across methods (p < 0.05). FTIR analysis revealed that extraction methods did not alter the primary structural configuration of the pectins. However, molecular weights (Mws) varied significantly, from 0.84 to 1.21 × 103 kDa, and the degree of esterification varied from 46.82% to 51.79% (p < 0.05). Monosaccharide analysis identified both homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) pectic configurations in all pectins, predominantly comprising galacturonic acid (77.21–83.12 %mol) and rhamnose (8.11–9.51 %mol), alongside minor side-chain sugars. These properties significantly influenced pectin functionalities. In the aqueous state, a higher Mw impacted viscosity and emulsification performance, while a lower Mw enhanced antioxidant activities and promoted the prebiotic function of pectin (Lactis brevies growth). This study highlights the impact of extraction methods on dragon fruit peel pectin functionalities and their structure–function relationship, providing valuable insights into predicting dragon fruit peel's potential as a food-grade ingredient in various products. [ABSTRACT FROM AUTHOR]

The pitai tree (Hylocereus polyrhizus) is a cacti with great prominence in the current fruit market. It has high rusticity and tolerance to adverse soil and climate conditions and has potential for cultivation in semi-arid regions. Despite being rarely affected by pests, its commercial exploitation can be harmed if control measures are not carried out. Furthermore, phytosanitary products have not been registered for pest control in this crop. Due to this, knowledge of the insects that affect this crop is of fundamental importance for their correct control and the productive success of the orchard. The objective of this study was to carry out an entomofauna survey aiming to evaluate the occurrence of harmful insects in a dragon fruit orchard located in the Pernambuco hinterland. The evaluation took place through photographic records, collection and identification of insects using data in the literature. Records and collections were carried out only during the day. The insects observed were: caterpillar Aricoris campestris (Bates, 1868) (Lepidoptera: Riodinidae), ant Atta sexdens rubropilosa (Forel, 1908) (Hymenoptera: Formicidae), Stingless bee arapuá Trigona spinipes (Fabricius, 1793) (Hymenoptera: Apidae), aphid Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae). This work reports the first record of A. campestris, ant, arapuá and aphid caterpillars causing damage to the dragon fruit. [ABSTRACT FROM AUTHOR]

Introduction: The consumption of high fat and sugar can increase the risk of metabolic syndrome. Hylocereus polyrhizus is known to be able to lower total cholesterol levels in the blood. This study aims to determine the effect of Hylocereus polyrhizus extract on total cholesterol levels in Wistar rats with the metabolic syndrome model. Methods: This study used laboratory experimental research using a pretest and posttest with a control group design. The sample consisted of 30 Wistar rats according to the inclusion criteria which were divided into 5 groups: K1 (positive control), K2 (negative control), K3, K4, and K5 (treatment group). The treatment group was given Hylocereus polyrhizus extract with doses of 60, 120, and 180 mg/200grW. Total cholesterol levels were measured on the 8th, 36th, and 64th day. The data were analyzed using the parameterized One-way ANOVA test followed by Tukey's HSD posthoc test, paired T-test, and Pearson's correlation test. Results: The average total cholesterol after being given by Hylocereus polyrhizus extract in K3 (137 ± 2.82 mg/dL), K4 (109 ± 2.31 mg/dL), and K5 (100 ± 2.38 mg/dL) was lower than the negative control group. Data analysis showed significant differences (p<0.05) in total cholesterol levels before and after treatment in all groups. The Pearson correlation test explained that the variation dosage of Hylocereus polyrhizus extract had a very strong correlation with a positive correlation with total cholesterol levels. Conclusion: Induction of Hylocereus polyrhizus extract at doses of 60, 120, and 180 mg/200grW was able to significantly reduce total cholesterol levels. [ABSTRACT FROM AUTHOR]

Dragon fruit (Hylocereus sp.) is an exotic crop with a great potential for its cultivation in semi arid tracts throughout India. Its planting is mainly confined to harsh climates and degraded lands facing challenges in its establishment. Hence, availability of quality planting material is essential for attaining success. Due to long juvenile phase of the sexually propagated seedlings it can be propagated asexually by stem cuttings but proper rooting is not possible without exogenous auxin application. Hence, during 2022- 2023 a research was carried out at the Horticultural Experimental area of Khalsa College, Amritsar to standardize the concentration of plant growth regulators viz. IBA, NAA and PHB for rooting and success rate in stem cuttings comprising of sixteen treatments with three replications arranged in randomized block design. The results revealed that the stem cuttings treated with IBA 4000 ppm proved to be superior for the sprouting and survival of the cuttings resulting in the improved vegetative growth with profuse, longer, thicker and the heaviest roots. Hence, the cutting treatment of IBA 4000 ppm can be proposed for dragon fruit plant propagation to meet the market demand in India. [ABSTRACT FROM AUTHOR]

BACKGROUND: Growing concern over the potential adverse health effects of synthetic colorants has led to a rising utilization of plant‐derived pigments within the food industry. Purple betacyanins from red dragon fruits (Hylocereus polyrhizus) have been subjected to an environmentally sustainable purification process for natural colorants. RESULTS: The violet‐hued pigments were absorbed onto preconditioned chitin material, a biodegraded polymer derived from shrimp shells. Liquid chromatographic–electrospray ionization–tandem mass spectrometric analysis was employed to analyze the betalain pigment composition in the raw fruit extract, making comparisons between the adsorbed supernatant and desorbed solutions. Fourier transform infrared analysis, optical microscopy, and scanning electron microscopy were conducted on the resin pre‐ and post‐adsorption stages, revealing distinctive infrared bands (RNHR, CO, CO, OH) indicative of betacyanin functional groups in the pigmented materials. The uniform adsorption of the colorant onto the adsorbents causes the intense purple color of the resin, with a maximum removal efficiency of 0.29 mg g−1. Adsorption data were subjected to various kinetic models, with the pseudo‐second‐order model demonstrating the optimal fit to the kinetic data (R2 = 0.97), while the Freundlich model proved most suitable for describing the sorption isotherm (R2 = 0.98). The adsorption energy, as determined by the Dubinin–Radushkevich equation, was 0.23 kJ mol−1, implying a physical adsorption mechanism on a heterogeneous surface. CONCLUSION: Betacyanins derived from red dragon fruits could be recovered for food colorants through chitin adsorption. This recovery mechanism is attributed to electrostatic interactions among the functional groups, including hydroxyl and carboxyl groups, and hydrogen bonds. © 2024 Society of Chemical Industry (SCI). [ABSTRACT FROM AUTHOR]

Red dragon fruit (RDF; Hylocereus polyrhizus L.) contains various polyphenols with potent antioxidant properties. Purée extracted from RDF has a vibrant red hue, making it a valuable natural food colouring agent suitable for a wide range of culinary applications. To preserve this valuable purée, non-thermal pasteurisation methods, such as highpressure processing (HPP), have emerged as an alternative to thermal pasteurisation (TP), as they do not impart the adverse effects associated with heat treatment. Therefore, the primary objective of the present work was to compare the impact of HPP and TP on several key attributes of RDF purée during 60-d storage. These attributes included the total betacyanin content (TBC), total phenolic content, total flavonoid content (TFC), antioxidant activities, enzyme activities, microbial growth, and colour stability. The RDF purée samples were divided into three groups: TP-treated (65°C/20 min), HPP-treated (350 MPa/5 min), and an untreated control group. All samples were stored at a controlled temperature of 4 ± 1°C, and analysed at 15-d intervals. Results revealed that preservation method, storage duration, and their interactions, significantly influenced the various parameters studied in RDF purée. Notably, HPP demonstrated superior efficacy in extending the shelf life of RDF purée well beyond 60 d, outperforming both TP and Control. Specifically, TP was proven effective in maintaining the phenolic content, antioxidant activities, and colour stability of the purée. On the other hand, HPP was particularly efficient in suppressing microbial growth and reducing enzyme activities in RDF purée. The findings can potentially transform the way RDF purée is preserved and utilised in the food industry, benefiting both producers and consumers, and contributing to more sustainable and health-conscious food practices. [ABSTRACT FROM AUTHOR]

Skin microbiota, and its diversity and balance, play a key role in skin health and beauty, influencing skin moisture, barrier function, and radiance. A healthy skin microbiota limits the growth of detrimental species, protecting the skin from pathologies. Prebiotics can support beneficial populations in outcompeting detrimental ones. Dragon fruit (Hylocereus undatus) contains prebiotic polysaccharides effective on gut bacteria. Its extract was tested in vitro, in a coculture model including representative beneficial and detrimental species, and in double-blind, placebo-controlled clinical trials. Effects on the skin microbiota were measured via 16S rDNA sequencing, and skin health and beauty benefits were evaluated through image analysis, TEWL measurement, and chromametry. Doppler flowmetry measured skin resilience. The extract supported S. epidermidis and S. hominis (beneficial species), while limiting S. aureus and C. acnes (representing pathogens) in vitro. Clinical results demonstrated its beneficial effects on skin microbiota diversity, especially in older volunteers (Faith's index up to +20% vs. placebo). Improvements were shown in skin sensitivity and resilience (by ca. 30% vs. placebo), skin redness (reflecting inflammation status), pigmentation and radiance (+11% ITA), barrier function (−13% TEWL), and wrinkling. This demonstrates this extract's positive effects on the beauty, health, and microbiota balance of the skin. [ABSTRACT FROM AUTHOR]

Every year, during the monsoon season, dengue, an infectious disease transmitted by the Aedes aegypti mosquito and caused by the dengue virus, occurs. The frequency and prevalence of dengue fever, as well as deaths from its consequences, have increased dramatically in recent years. Despite the fact that the infection has affected a large number of people worldwide, no specific and efficient drug treatment is known. Researchers are working tirelessly to find a cure, focusing their efforts on developing therapeutic treatments that block or inhibit the activity of specific Dengue virus (DENV) proteins. This review focuses on the phytochemical constituents present in traditionally used natural remedies for the treatment of dengue such as papaya leaf extract, goat milk, kiwi fruit, dragon fruit, and coconut water. Undoubtedly, the contents of this review will motivate researchers to do more novel work in this area which will accelerate the journey towards the exploration of the pharmacological activity of natural compounds. [ABSTRACT FROM AUTHOR]

The herb Hylocereus undatus, which is rich in phytochemicals and thought to contain antioxidants comparable to those in its peel, has a lot of promise for use in the food field. The presented study aimed to study the protective effect of the aqueous extrac and the active compounds (gallic acid) isolated from the dragon fruit (H. undates) to lessen the impact of rheumatoid arthritis (RA) induced by Freund's Complete Adjuvant (CFA)) by studying some of the changes in biochemical and histochemical parameters. The Wistar albino rats (male) were divided into four groups viz., Group I: A negative control group, dosed only with plain water orally, Group II: Induced for RA by CFA, a positive control group; Group III: dosed with aqueous extract of the fruit of H. undatus (kg/500 mg); and Group IV: dosed with gallic acid isolated from this fruit (kg/mg20). The results showed a significant increase in malondialdehyde (MDA), platelet, interleukin 6 (IL-6), and RF in rats treated with CFA and a significant decrease in Hb compared to the negative control group. There was a significant decrease of MDA, Platelet, IL-6, and RF in the groups treated with aqueous extract and a significant increase in Hb compared with the positive control group while there was a significant decrease in each of MDA, platelets, IL-6, and RF. Based on a histological analysis, the study group's liver and kidneys had smaller lesions and different abnormalities than the control. Thus, the fruit plant's gallic acid extracts were protective in reducing RA damage caused by CFA and improving kidney and liver tissue. [ABSTRACT FROM AUTHOR]

Pitaya fruit has highly edible, medicinal, and economic value. Currently, pitaya is often used as fresh fruit to meet the market demand of consumers. However, pitaya fruit is prone to mechanical damage, microbial infection, and softening and decay after harvest, affecting the quality and shelf life of fresh pitaya fruit. Therefore, many technologies have been developed to extend the postharvest storage time of fresh pitaya. This review summarises the internal and external factors that affect the deterioration in quality of pitaya fruit after harvest and introduces the types of preservation technologies for pitaya fruit presently available. This includes physical, chemical, and techniques based on natural substances to extend the shelf life of postharvest pitaya fruit, as well as the control methods of postharvest diseases and pests. Future research needs to consider the combined application of various technologies to improve the preservation of pitaya. [ABSTRACT FROM AUTHOR]