1. Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
- Author
-
Bengoa Luoni, Sofia A., Cenci, Alberto, Moschen, Sebastian, Nicosia, Salvador, Radonic, Laura M., Sabio, Julia, Garcia, Langlade, Nicolas B., Vile, Denis, Rovere, Cecilia Vazquez, Fernandez, Paula, Instituto Nacional de Tecnología Agropecuaria (INTA), Bioversity International [Montpellier], Bioversity International [Rome], Consultative Group on International Agricultural Research [CGIAR] (CGIAR)-Consultative Group on International Agricultural Research [CGIAR] (CGIAR), Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INTA PE 1131022, 1131043, ANPCyT Préstamo BID PICT 2012–0390, PICT 2014–0175, PIP CONICET PIP 11220120100262CO, Marie Curie IRSES Project DEANN (PIRSES-GA-2013-612583), CONICET, SUNRISE and Heliagen Project, and ANR-11-BTBR-0005,SUNRISE,Ressources génétiques de tournesol pour l'amélioration de la stabilité de production d'huile sous c(2011)
- Subjects
0106 biological sciences ,Leaves ,Arabidopsis thaliana ,Arabidopsis ,QH426-470 ,Senescence ,01 natural sciences ,03 medical and health sciences ,Leaf senescence ,Factores de Transcripción ,Gene Expression Regulation, Plant ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Genetics ,Girasol ,Phylogeny ,030304 developmental biology ,Plant Proteins ,2. Zero hunger ,0303 health sciences ,Leaf Senescence ,Research ,High throughput phenotyping ,Correction ,food and beverages ,Helianthus annuus ,Phylogenetic Analysis ,15. Life on land ,HaNAC001 ,Genética ,Plant Senescence ,Plant Leaves ,Sunflower ,Análisis Filogenético ,NAC transcriptions factors ,Avejentamiento ,Hojas ,Helianthus ,TP248.13-248.65 ,010606 plant biology & botany ,Biotechnology ,Transcription Factors - Abstract
Background: Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. Results: To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. Conclusions: This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower. Instituto de Biotecnología Fil: Bengoa Luoni, Sofia Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Cenci, Alberto. Bioversity International; Francia Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Fil: Nicosia, Salvador. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Langlade, Nicolas B. Université de Toulouse. LIPM-INRA-CNRS; Francia Fil: Langlade, Nicolas B. Université de Montpellier. LEPSE; Francia Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
- Published
- 2021
- Full Text
- View/download PDF