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Microvascular endothelial cells (MiVECs) impair angiogenic potential, leading to microvascular rarefaction, which is a characteristic feature of chronic pressure overload-induced cardiac dysfunction. Semaphorin3A (Sema3A) is a secreted protein upregulated in MiVECs following angiotensin II (Ang II) activation and pressure overload stimuli. However, its role and mechanism in microvascular rarefaction remain elusive. The function and mechanism of action of Sema3A in pressure overload-induced microvascular rarefaction, is explored, through an Ang II-induced animal model of pressure overload. RNA sequencing, immunoblotting analysis, enzyme-linked immunosorbent assay, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and immunofluorescence staining results indicate that Sema3A is predominantly expressed and significantly upregulated in MiVECs under pressure overload. Immunoelectron microscopy and nano-flow cytometry analyses indicate small extracellular vesicles (sEVs), with surface-attached Sema3A, to be a novel tool for efficient release and delivery of Sema3A from the MiVECs to extracellular microenvironment. To investigate pressure overload-mediated cardiac microvascular rarefaction and cardiac fibrosis in vivo, endothelial-specific Sema3A knockdown mice are established. Mechanistically, serum response factor (transcription factor) promotes the production of Sema3A; Sema3A-positive sEVs compete with vascular endothelial growth factor A to bind to neuropilin-1. Therefore, MiVECs lose their ability to respond to angiogenesis. In conclusion, Sema3A is a key pathogenic mediator that impairs the angiogenic potential of MiVECs, which leads to cardiac microvascular rarefaction in pressure overload-induced heart disease. in press, CA extern

In order to improve the utilization rate of nitrogen fertilizer and reduce the environmental pollution risk of the nitrogen accumulation in the vegetable field, this study was carried out in the summer leisure period of the greenhouse vegetable production. This experiment designed different planting density treatments in 2017 and 2018, i.e. for catch waxy corn, 3300 plants/667m2 (WCD1), 5000 plants/667m2 (WCD2), 6600 plants/667m2 (WCD3), for forage sweet sorghum, 4500 plants /667m2 (FSS4), 7000 plants/667m2 (FSS5), 9000 plants/667m2 (FSS6) in 2017; and for catch waxy corn, 4500 plants/667m2 (WCDI), 7000 plants/667m2 (WCDII), 9000 plants/667m2(WCDIII), for forage sweet sorghum, 7000 plants/667m2 (FSSIV), 10000 plants/667m2 (FSSV), 14000 plants/667m2 (FSSVI) in 2018. The results showed that the biomass and nitrogen absorption of the two catch crops began to improve and then decreased with the increase of planting density. The nitrogen absorption amount of the catch waxy corn and forage sweet sorghum was 22.36~28.68 kg/667m2,21.67~24.39 kg/667m2, respectively. Different planting density of catch waxy corn and forage sweet sorghum could significantly reduce the total nitrogen content of 0~30cm soil layer and the nitrate nitrogen content of 0~90cm soil layer, for catch waxy corn and forage sweet sorghum, the reduction rate of total nitrogen content in 0~30cm soil layer was 9.6%~27.0%, 5.7%~23.5%, the reduction rate of nitrate nitrogen content reached 50.0%~90.8%, 80.1%~96.4%, respectively, which effectively controlled the nitrate nitrogen leaching to soil deep layer. Planting catch crops could increase soil urease activity, regulate soil nitrogen transformation. Compared with other treatments, WCDII and FSSV treatment can reduce the initial urease activity and soil nitrate nitrogen content of next crops, which is consistent with the nutrient requirements of broccoli in the early stage of growth. These catch crops planting could reduce the nitrogen environmental risk in the greenhouse soil. Finally, the study proposed that the suitable planting density of catch waxy corn and forage sweet sorghum planted was 6600~7000 plants/667m2, and 9000~10000 plants/667m2, respectively, in the greenhouse summer leisure period. It is more advantageous to improve soil nitrogen absorption and reduce soil nitrogen environmental risks for catch waxy corn.

In this experiment, the effects of different types of conditioners and their application on the absorption and transformation of phosphorus in high phosphorus soils in facilities were investigated to improve the environmental problems of protected farmland caused by phosphorus accumulation. Waxy corn was used as a test crop, and five conditioners such as humic acid, biochar, bentonite, alum, and dephosphorized gypsum were used as test materials for potting experiments. The experiment set 10 treatments, namely T1(Blank control), T2(Humic acid), T3(Biochar), T4(Bentonite), T5(Alum), T6 (Dephosphorized gypsum), T7(Biochar-bentonite-alum), T8(Humic acid-biochar-alum),T9(Humic acidbiochar- bentonite-alum),T10(Humic acid-bentonite-biochar-alum-dephosphorized gypsum). Based on the analysis of the results of the three crops, except that the first crop was not significant, the biomass and phosphorus absorption of waxy corn of T2 was the highest in the second crop, and T10 was the most effective treatment in the third crop. The soil available phosphorus content of T8 was the lowest in the second crop and that of T10 was the lowest in the third crop, which were 12.01% and 12.75% lower than the control. The soil water-soluble phosphorus content of T4 was the lowest in the second crop, which was 41.84% lower than the control, and that of T8 was the lowest in the third crop, which was 26.62% lower than the control. According to the results of the three crops, the ratio of the total phosphorus content of the inorganic phosphorus in the third crop of each treatment was increasing compared with the first crop. The soil phosphorus was transformed from organic phosphorus to inorganic phosphorus. The ratio of total phosphorus content of organic phosphorus of T6, T9, T10 was larger than other treatments, which slowed down the conversion of phosphorus to available form. The ratio of Ca8-P in the inorganic phosphorus was the highest, reaching about 50% to 60%. From the results and analysis, T2 and T10 were beneficial to the absorption of soil phosphorus by waxy corn, T8 and T10 were beneficial to slow down the conversion of phosphorus to the effective state, reduce the potential risk of phosphorus environment, improve the phosphorus accumulation environmental issues in greenhouse farmland.

Objectives MRI in the evaluation of the metabolic syndrome (metabolic syndrome, MS) on left ventricular function in hypertensive patients in clinical application. Methods the Materials and Methods 2.1, the study Collected in March 2011 June 2011 Taiyuan Central Hospital, Cardiology, Endocrinology, intervention wards and outpatient or inpatient treatment for patients with hypertension, repeated hospitalisation of patients with first hospitalisation prevail. According to the 2004 Chinese Medical Association Diabetes Branch of the Chinese population recommended for diagnostic criteria for metabolic syndrome (6): body mass index (BMI) ≥25 kg/m 2 ; triglyceride (TG) ≥1.7 mmol/l; high-density lipoprotein—cholesterol (HDL-C) M Check : Check the front again on the inspiratory and expiratory training patients, Zhu Huanzhe shallow breathing in breath-hold scan mode. First, check the pulse sequence applied TurboFlash2D cardiac axial and sagittal orientation scan, and then like on the axial position, parallel to the left ventricular outflow tract for positioning of the image and right ventricular outflow tract parallel to the positioning of images, the vertical the left and right ventricular outflow tract position as, in the heart of the Ministry of base, middle and apical part of the Department of breath do not be scanned across the surface FLASH pulse sequence. 2.23, image analysis Application of Siemens, Sonata workstation dedicated cardiac function measurement software (Argus) for measurement of cardiac function, the application of semi-automatic depicting left ventricular short axis endocardial and epicardial contours. Obtained left ventricular volume—time curves, calculated left ventricular (LV) heart function parameters: Left ventricular end-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), cardiac output (CO), ejection fraction (EF), myocardial mass (MM), and left ventricular peak ejection rate (PER), peak ejection time (TPER), peak filling rate (PFR), time to peak filling (TPFR), increased wall thickness ratio (TN). 2.24, statistical analysis Analysis using SPSS14.0 statistical package. Measurement data with the mean±SD (±s) that the differences between the groups of data into a separate group t test. Count data using χ 2 test, test level to take α=0.05, p Results In normal control group, hypertension, high blood pressure metabolic syndrome group differences were statistically significant (p Conclusions HMS and EH can cause changes in left ventricular function, and metabolic dysfunction may further aggravate this change; MRI can accurately determine the film HMS, EH patients with left ventricular heart function parameters, and good reproducibility.

International audience; A three-in-one ITO-Cu-ITO multilayer wire strategy is developed to reliably fabricate large-size backplane circuit for micro-LED display. The multilayered wire was prepared by magnetron sputtering and electroplating, and presented a sandwich structure of ITOtransitional layer /Cuseed layer /Cuelectroplating layer/ITOpassivation layer. Synergistic effect among the sandwiched layers endowed the coated wire with good electrical performance (2.31 × 10-3 Ω/sq), favorable adhesion (5B degree) and superior thickness uniformity (8 %), and could ensure reliable machining of glass-based backplane circuit. The fabricated multilayer wire facilitated the development of micro-LED backplane circuit, and was applicable to synthesize large-size (1.1 * 1.3 m2) glass backplane circuit. Field test of micro-LED circuit assembly proved the feasibility of the proposed backplane circuit strategy in production line. It is rational to believe that our strategy may offer a good avenue to upgrade traditional ITO and Cu materials for developing high-performance displays in scale production.