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The aim of the present study was to assess the innocuity of isoflurane in rat lung and liver through histopathological examination. Experimental protocol: 20 female Wistar rats were divided intro 4 groups, each consisting of 5 individuals: control group, group I, II and III. Female rats from the control group were not exposed to any anesthetic treatment, while female rats from the other groups (I, II and III) were exposed to isoflurane three times, for 2 hours, every other day. Animals from the control group and group I were sacrificed immediately after the last exposure (0 h). Group II was sacrificed 6 hours after the last exposure and group III 24 hours after the last exposure. Fragments of the lung and liver were collected from the 20 female rats taken into study and then histologically processed (fixed in 10% buffered formalin and embedded in paraffin). After obtaining 5 μm thick sections, they were stained using Goldner's Trichrome method. Histopathological examination of the tissue specimens showed no structural changes perceptible through light microscopy, thus showing that isoflurane did not exert a toxic action in these two organs. The present study proved a good innocuity of isoflurane in rat lung and liver. [ABSTRACT FROM AUTHOR]