33,658 results on '"PC12 Cells"'
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2. 加味孔圣枕中丹含药血清调控 SIRT1/PGC-1α 通路改善氧糖剥夺 再灌注 PC12 细胞的线粒体功能.
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吴俏兰, 欧春雪, 武筱林, 高祖, 王嘉昀, and 于华芸
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ISCHEMIC stroke ,FLOW cytometry ,OXYGEN consumption ,APOPTOSIS ,RESPIRATION - Abstract
Copyright of Traditional Chinese Drug Research & Clinical Pharmacology is the property of Traditional Chinese Drug Research & Clinical Pharmacology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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3. Neurotrophic Natural Products
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Fukuyama, Yoshiyasu, Kubo, Miwa, Harada, Kenichi, Kinghorn, A. Douglas, Series Editor, Falk, Heinz, Series Editor, Gibbons, Simon, Series Editor, Asakawa, Yoshinori, Series Editor, Liu, Ji-Kai, Series Editor, Dirsch, Verena M., Series Editor, Appendino, Giovanni, Advisory Editor, Kobayashi, Jun'ichi, Advisory Editor, Ludwiczuk, Agnieszka, Advisory Editor, Naman, C. Benjamin, Advisory Editor, Mata, Rachel, Advisory Editor, and Trauner, Dirk, Advisory Editor
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- 2024
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4. Protective Effect of Nervonic Acid on Oxidative Damage of PC12 Cells Induced by H2O2
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Mayile AIHAITI, LIU Yaojie, LI Jianke
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nervonic acid ,oxidative damage ,pc12 cells ,signaling pathway ,Food processing and manufacture ,TP368-456 - Abstract
This study developed a cell model of oxidative damage by treating PC12 cells for 24 h with 200 µmol/L H2O2 and determined the degree of oxidative stress by assaying the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and lactate dehydrogenase (LDH) and the level of malondialdehyde (MDA). Moreover, cell apoptosis and reactive oxygen species (ROS) levels were evaluated. Western blot and real-time polymerase chain reaction (PCR) were used to detect the protein and mRNA expression levels of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), caspase-3, nuclear factor E2 related factor 2 (Nrf2), kelch-like ECH-associated protein 1 (Keap1), and heme oxygenase-1 (HO-1). The results showed that after being treated with 200 µmol/L H2O2 for 24 h, the survival rate of PC12 cells was 60.12%. Cytotoxicity experiments showed that nervonic acid could significantly reduce the contents of LDH and MDA, inhibit excessive production of ROS, and enhance the activities of SOD and GSH-Px in H2O2-injuried cells. In addition, it significantly upregulated the expression of Bcl-2, Nrf2 and HO-1, and downregulated the expression of caspase-3, Bax, and Keap1. In summary, nervonic acid has a protective effect on H2O2-induced oxidative damage in PC12 cells by a mechanism associated with the activation of the Nrf2/HO-1 signaling pathway.
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- 2024
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5. A PEDOT: PSS/GO fiber microelectrode fabricated by microfluidic spinning for dopamine detection in human serum and PC12 cells.
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Zhao, Zexu, Hou, Yang, Zhang, Hao, Guo, Jiahao, and Wang, Jinyi
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MICROFIBERS , *DOPAMINE , *FIBERS , *DETECTION limit , *MICROELECTRODES , *NEUROLOGICAL disorders - Abstract
Rapid and accurate in situ determination of dopamine is of great significance in the study of neurological diseases. In this work, poly (3,4-ethylenedioxythiophene): poly (styrenesulfonic acid) (PEDOT: PSS)/graphene oxide (GO) fibers were fabricated by an effective method based on microfluidic wet spinning technology. The composite microfibers with stratified and dense arrangement were continuously prepared by injecting PEDOT: PSS and GO dispersion solutions into a microfluidic chip. PEDOT: PSS/GO fiber microelectrodes with high electrochemical activity and enhanced electrochemical oxidation activity of dopamine were constructed by controlling the structure composition of the microfibers with varying flow rate. The fabricated fiber microelectrode had a low detection limit (4.56 nM) and wide detection range (0.01–8.0 µM) for dopamine detection with excellent stability, repeatability, and reproducibility. In addition, the PEDOT: PSS/GO fiber microelectrode prepared was successfully used for the detection of dopamine in human serum and PC12 cells. The strategy for the fabrication of multi-component fiber microelectrodes is a new and effective approach for monitoring the intercellular neurotransmitter dopamine and has high potential as an implantable neural microelectrode. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Myosin VI in the nucleolus of neurosecretory PC12 cells: its involvement in the maintenance of nucleolar structure and ribosome organization.
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Nowak, Jolanta, Lenartowski, Robert, Kalita, Katarzyna, Lehka, Lilya, Karatsai, Olena, Lenartowska, Marta, and Rędowicz, Maria Jolanta
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NUCLEAR proteins ,NUCLEOLUS ,MOLECULAR motor proteins ,MYOSIN ,CELL motility - Abstract
We have previously shown that unconventional myosin VI (MVI), a unique actin- based motor protein, shuttles between the cytoplasm and nucleus in neurosecretory PC12 cells in a stimulation-dependent manner and interacts with numerous proteins involved in nuclear processes. Among the identified potential MVI partners was nucleolin, a major nucleolar protein implicated in rRNA processing and ribosome assembly. Several other nucleolar proteins such as fibrillarin, UBF (upstream binding factor), and B23 (also termed nucleophosmin) have been shown to interact with MVI. A bioinformatics tool predicted the presence of the nucleolar localization signal (NoLS) within the MVI globular tail domain, and immunostaining confirmed the presence of MVI within the nucleolus. Depletion of MVI, previously shown to impair PC12 cell proliferation and motility, caused disorganization of the nucleolus and rough endoplasmic reticulum (rER). However, lack of MVI does not affect nucleolar transcription. In light of these data, we propose that MVI is important for nucleolar and ribosome maintenance but not for RNA polymerase 1-related transcription. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Protection of Si Nanowires against A β Toxicity by the Inhibition of A β Aggregation.
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Zhao, Xuechun, Mou, Chenye, Xu, Jiayi, Cui, Wei, Shi, Yijing, Wang, Yangzhe, Luo, Tian, Guo, Wei, Ye, Jichun, and Chen, Wanghua
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PLASMA-enhanced chemical vapor deposition , *NANOWIRES , *ALZHEIMER'S disease , *NEURONS , *BIOCOMPATIBILITY , *GOLD nanoparticles - Abstract
Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by the accumulation of amyloid beta (Aβ) plaques in the brain. Aβ1–42 is the main component of Aβ plaque, which is toxic to neuronal cells. Si nanowires (Si NWs) have the advantages of small particle size, high specific surface area, and good biocompatibility, and have potential application prospects in suppressing Aβ aggregation. In this study, we employed the vapor–liquid–solid (VLS) growth mechanism to grow Si NWs using Au nanoparticles as catalysts in a plasma-enhanced chemical vapor deposition (PECVD) system. Subsequently, these Si NWs were transferred to a phosphoric acid buffer solution (PBS). We found that Si NWs significantly reduced cell death in PC12 cells (rat adrenal pheochromocytoma cells) induced by Aβ1–42 oligomers via double staining with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and fluorescein diacetate/propyl iodide (FDA/PI). Most importantly, pre-incubated Si NWs largely prevented Aβ1–42 oligomer-induced PC12 cell death, suggesting that Si NWs exerts an anti-Aβ neuroprotective effect by inhibiting Aβ aggregation. The analysis of Fourier Transform Infrared (FTIR) results demonstrates that Si NWs reduce the toxicity of fibrils and oligomers by intervening in the formation of β-sheet structures, thereby protecting the viability of nerve cells. Our findings suggest that Si NWs may be a potential therapeutic agent for AD by protecting neuronal cells from the toxicity of Aβ1–42. [ABSTRACT FROM AUTHOR]
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- 2024
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8. 神经酸对H2O2c诱导PC12细胞氧化 损伤的保护作用.
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玛依乐·艾海提 and 刘垚杰,李建科
- Abstract
Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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9. Zirconium dioxide nanoparticles induced cytotoxicity in rat cerebral cortical neurons and apoptosis in neuron-like N2a and PC12 cell lines.
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Alavi, Mohaddeseh Sadat, Asadpour, Elham, Boroushaki, Mohammad Taher, Fakharzadeh Moghadam, Omid, and Sadeghnia, Hamid R
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ZIRCONIUM oxide , *CYTOTOXINS , *CELL lines , *ORAL drug administration , *WESTERN immunoblotting , *NANOMEDICINE , *BLOOD-brain barrier - Abstract
During recent decades, the application of zirconium dioxide nanoparticles (ZrO2-NP) has been expanded in various fields ranging from medicine to industry. It has been shown that ZrO2-NP has the potential to cross the blood–brain barrier (BBB) and induce neurotoxicity. In the current study, we investigated the in vivo neurotoxicity, as well as, the cellular mechanism of ZrO2-NP toxicity on two neuronal-like cell lines, PC12 and N2a. PC12 and N2a cells were exposed to increasing concentrations of ZrO2-NP (0-2000 µg/ml) for 48 h. The apoptotic effect of ZrO2-NP was determined using annexin V/propidium iodide double staining (by flow cytometry), and western blot analysis of relative apoptotic proteins, including caspase-3, caspase-9, bax, and bcl2. Based on our results, ZrO2-NP at concentrations of 250-2000 μg/mL increased both early and late-stage apoptosis in a concentration-dependent manner. Moreover, the expressions of cleaved-caspase-3 and -9 proteins and the bax/bcl2 ratio were significantly increased. In addition, oral administration of ZrO2-NP (50 mg/kg) to male Wistar rats for 28 days led to the loss of neuronal cells in the cerebral cortex. Taken together, our findings highlighted the role of apoptosis on cytotoxicity induced by ZrO2-NP. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Progesterone improved the behavior of PC12 cells under OGD/R by reducing FABP5 expression and inhibiting TLR4/NF-κB signaling pathway.
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Li, Chunlin, Li, Bowen, Qu, Linglong, Song, Ruichang, Liu, Hui, and Su, Shanshan
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PROGESTERONE , *PROGESTERONE receptors , *CELLULAR signal transduction , *CELL proliferation , *WESTERN immunoblotting , *CELL survival - Abstract
Herein, PC12 cells were applied to detect the impact of progesterone under oxygen glucose deprivation/reperfusion (OGD/R) stimulation. The cell proliferation of PC12 cells was evaluated by cell counting kit-8 assay, and the concentrations of MDA, ROS and SOD were examined by their corresponding Enzyme Linked Immunosorbent Assay kits. The invasion and migration properties of PC12 cells were evaluated by transwell and wound healing assays, respectively. The expression patterns of related genes were evaluated by western blot and qPCR. Under OGD/R stimulation, progesterone treatment could elevate the viability of PC12 cells, reduce the levels of MDA and ROS, and elevate the concentration of SOD. Moreover, progesterone treatment could strengthen the invasion and migration abilities of PC12 cells under OGD/R condition, as well as decrease the apoptosis and inflammation. FABP5 expression was significantly increased in PC12 cells under OGD/R stimulation, which was reversed after progesterone stimulation. Under OGD/R stimulation, the protective effects of progesterone on PC12 cells were strengthened after si-FABP5 treatment. The protein levels of TLR4, p-P65 NF-κB, and P65 NF-κB in OGD/R-induced PC12 cells were increased, which were inhibited after progesterone treatment. Progesterone exerted protective effects on PC12 cells by targeting FABP5 under OGD/R stimulation. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Knocking down GRAMD1C expression reduces 6-OHDA-induced apoptosis in PC12 cells.
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He, Hui, Zhang, Bo, Wang, Xiang, and Chen, Lulu
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PARKINSON'S disease ,APOPTOSIS ,FLOW cytometry ,CELL proliferation ,WESTERN immunoblotting - Abstract
Aim To explore the differential genes in Parkinson's disease (PD) through a preliminary GEO database, and to investigate the possible mechanisms. Materials and Methods The PD differentially expressed genes (DEGs) were analyzed by the microarray method. Then, these DEGs were applied to KEGG and GO analyses to predict the related signaling pathways and molecular functions. Comparison of GRAMD1C expression levels in the putamen of normal and Parkinson's patients by bioinformatic analysis. PC12 cells were cultured to construct a 6-hydroxydopamine (6-OHDA)-induced Parkinson's cell model. RT-qPCR was performed to detect the efficiency of GRAMD1C siRNA. MTT assay was conducted to examine the proliferation of cells. Then, the apoptosis of each group of cells was measured by flow cytometry. Western blot was carried out to determine the expression of apoptosis-related proteins. Results Through bioinformatics, GRAMD1C was confirmed to be one of the most significantly upregulated genes in PD. Furthermore, GRAMD1C was notably enhanced in the PD patients and 6-OHDA-induced PC12 cells. Besides, 6-OHDA stimulation significantly reduced PC12 cell proliferation, and it reverted with the GRAMD1C siRNA. Moreover, the flow cytometry results showed that knockdown of GRAMD1C could effectively reduce the high apoptosis rate of PC12 cells induced by 6-OHDA treatment. Similarly, western blot results found that 6-OHDA stimulation markedly increased the expression levels of Bax and Caspase 3Caspase 3 and decreased the Bcl-2 expression in PC12 cells, and GRAMD1C knockdown reversed these changes. Conclusion GRAMD1C is upregulated in PD, and may affect the PD process through the apoptotic pathway. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Chlorogenic acid permeation across intestinal cell monolayers: Influence by circadian rhythms in the presence of other natural polyphenols and by dopaminergic neuronal-like cells
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Giada Botti, Barbara Pavan, Anna Bianchi, Luca Ferraro, Sarah Beggiato, Federica Brugnoli, Valeria Bertagnolo, and Alessandro Dalpiaz
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Chlorogenic acid ,Circadian rhythms ,IEC-6 cells ,Intestinal permeability ,Natural polyphenols ,PC12 cells ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Poor oral bioavailability limits chlorogenic acid (CGA) benefits on the gut-brain axis. In this study we demonstrate the existence of an active circadian-dependent efflux for the CGA intestinal permeation, leading to greater bioavailability in the evening rather than in the morning. As therapeutic/nutraceutical polyphenols can mutually influence their intestinal absorption following circadian rhythms, we also evaluated the effects of other polyphenols on CGA permeation/bioavailability. The results indicate that gallic acid reduced in vitro CGA permeation across IEC-6 cell monolayers, as well as rat oral CGA bioavailability. On the contrary, arbutin, caffeic acid and ferulic acid did not modify in vitro CGA permeation. Finally, 60 mM KCl-evoked dopamine release from PC12 cells significantly increased intestinal CGA permeation, likely by downregulating the expression/activity of efflux transporters, as confirmed by western blot analysis. Dopamine released from the enteric nervous system may therefore increase the dependence of oral bioavailability of CGA on circadian rhythms.
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- 2024
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13. Anti-inflammatory and DNA Repair Effects of Astragaloside IV on PC12 Cells Damaged by Lipopolysaccharide
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Li, Hai-long, Shao, Li-hua, Chen, Xi, Wang, Meng, Qin, Qi-jie, Yang, Ya-li, Zhang, Guang-run, Hai, Yang, and Tian, Yi-hong
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- 2024
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14. miR-497-5p promoted neuronal injury in ischemic stroke by inhibiting the BDNF/TrkB/PI3K/Akt pathway.
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Chunyan Gong, Xiaona He, Guiliang Li, Dayu Wang, Yonghua Yang, Yanping Shi, Wenbing Su, and Yuanxian Wu
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ISCHEMIC stroke ,BRAIN-derived neurotrophic factor ,REACTIVE oxygen species ,WOUNDS & injuries ,CELL survival - Abstract
The aim of this study was to investigate the molecular mechanism by which miR-497-5p regulates neuronal injury after ischemic stroke through the BDNF/TrkB/Akt signaling pathway. PC12 cells were used to construct a stroke injury model by oxygen-glucose deprivation/reoxygenation (OGD/R). The expression level of miR-497-5p was measured by RT-qPCR. CCK-8 kit was used to detect cell viability. Cell apoptosis and reactive oxygen species (ROS) were detected by flow cytometry. MDA and SOD detection kits were used to detect MDA content and SOD activity. A double luciferase reporter system was used to verify the targeting relationship between miR-497-5p and BDNF. The expression of BDNF, TrkB, p-TrkB, Akt and p-Akt was detected by Western blot. We have found that miR-497-5p expression was inhibited after treatment with OGD/R. Simultaneously, cell apoptosis, MDA content and ROS were upregulated, while cell viability and SOD were significantly decreased in PC12 cells. The effects of OGD/R on PC12 cells were reversed with the downregulation of miR-497-5p. A double luciferase reporter assay demonstrated that miR-497-5p negatively targets BDNF. BDNF inhibited cell apoptosis and oxidative stress injury in PC12 cells. These findings suggest that miR-497-5p aggravates neuronal injury in experimental model of ischemic stroke by inhibiting the BDNF/TrkB/PI3K/Akt signaling pathway. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Potential of Collagen/PLA-Based Nanofibrous Scaffold to Support PC12 Cells and Neural Repair.
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Aseer, Muhammad, Taleb, Mohammad, Arabpour, Zohreh, Bhatti, Qaisar Abbas, and Ghanbari, Hossein
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BIOPOLYMERS ,POLYLACTIC acid ,NERVE tissue ,CELL adhesion ,PERIPHERAL nervous system - Abstract
Objective: This study attempts to modify Polylactic Acid (PLA) with the natural polymer Collagen (Coll), to develop materials such as an electrospun scaffold that have better mechanical stability and biocompatibility. Retinoic acid (RA), a bioactive material that promotes nerve growth, is to be added to the nanofiber scaffolds as part of this project. Methods: One of the most important methods we employed in this work to create nanofibrous scaffolds was electrospinning. Results: The synthesized nanofiber scaffold exhibited a diameter of 255 ± 40 nm and a tensile strength of 175 ± 10.4 N, providing sufficient support for native peripheral nerve repair. The inclusion of Coll enhanced the scaffold's hydrophilic behavior (contact angle: 56 ± 4°), ensuring stability in aqueous solutions. In addition, cell adhesion and proliferation are demonstrated to be improved by PLA composite nanofibers based on Collagen, while PC12 cell adhesion and proliferation are further improved by RA. Conclusion: Based on their biodegradability, robust mechanical properties, and porous structure, these scaffolds are excellent choices for nerve tissue engineering, according to our findings. The significant increase in PC12 cells' adhesion and proliferation upon the addition of RA demonstrates the cells' potential for nerve repair. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Characterization and Exploration of the Neuroprotective Potential of Oat-Protein-Derived Peptides in PC12 Cells and Scopolamine-Treated Zebrafish.
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Rafique, Hamad, Hu, Xinzhong, Ren, Tian, Dong, Rui, Aadil, Rana Muhammad, Zou, Liang, Sharif, Mian Kamran, and Li, Lu
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Neurodegenerative disorders pose a substantial risk to human health, and oxidative stress, cholinergic dysfunction, and inflammation are the major contributors. The purpose of this study was to explore the neuroprotective effects of oat protein hydrolysate (OPH) and identify peptides with neuroprotective potential. This study is the first to isolate and identify OPH peptides with neuroprotective potential, including DFVADHPFLF (DF-10), HGQNFPIL (HL-8), and RDFPITWPW (RW-9), by screening via peptidomes and molecular-docking simulations. These peptides showed positive effects on the activity of antioxidant enzymes and thus reduced oxidative stress through regulation of Nrf2-keap1/HO-1 gene expression in vitro and in vivo. The peptides also significantly ameliorated scopolamine-induced cognitive impairment in the zebrafish model. This improvement was correlated with mitigation of MDA levels, AChE activity, and levels of inflammatory cytokines in the brains of zebrafish. Furthermore, these peptides significantly upregulated the mRNA expression of Bdnf, Nrf2, and Erg1 in the brains of zebrafish with neurodegenerative disorders. Collectively, oat peptides have potential for use as active components in nutraceutical applications for the prevention of neurodegenerative diseases. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Simple ammonium salt and sigma-1 receptor ligand dipentylammonium provides neuroprotective effects in cell culture and Caenorhabditis elegans models of Alzheimer's disease
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Mani Iyer Prasanth, Kanika Verma, Sirikalaya Brimson, Tewin Tencomnao, and James Michael Brimson
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σ-1R ,DPA ,Neurite outgrowth ,PC12 cells ,Amyloid-β ,C. elegans ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The sigma-1 receptor (σ-1R), a chaperone protein located at the mitochondria-associated membrane (MAM) of the endoplasmic reticulum, can interact with and modify the signaling pathways of various proteins, thereby modulating many disease pathologies, including Alzheimer's disease (AD). The σ-1R ligand dipentylammonium (DPA) was analyzed for its anti-AD properties using PC12 cells (in vitro) and Caenorhabditis elegans (in vivo) models along with molecular docking (in silico) analysis. DPA at 1 and 10 µM concentrations was able to significantly potentiate NGF-induced neurite growth length by 137.7 ± 12.0 and 187.8 ± 16.4, respectively, when compared to the control 76.9 ± 7.4. DPA also regulated neurite damage caused by Aβ(25−35) treatment in differentiated PC12 cells by improving cell viability and neurite length. In C. elegans, DPA could significantly extend the median and maximum lifespan of Aβ transgenic strain CL2006 without impacting wild-type nematodes. Additionally, it could significantly reduce the paralysis phenotype of another Aβ transgenic strain, CL4176, thereby improving the overall health in AD pathogenesis. This effect depended on σ-1R, as DPA could not modulate the lifespan of σ-1R mutant TM3443. This was further confirmed using agonist PRE084 and antagonist BD1047, wherein the agonist alone could extend the lifespan of CL2006, while the antagonist suppressed the effect of DPA in CL2006. Interestingly, neither had an TM3443. Further, molecular docking analysis showed that DPA had a similar binding affinity as that of PRE084, BD1047 and pentazocine against the σ-1R receptor in humans and C. elegans, which collectively suggests the anti-AD properties of DPA.
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- 2024
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18. PROTECTIVE EFFECT OF NEUREGULIN1β ON OXYGEN-GLUCOSE DEPRIVATION/REOXYGENATION-INDUCED PC12 CELL INJURY AND ITS MECHANISM
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SUN Shanshan, YU Xi, ZHAI Qiuyue, YU Zhuqin
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neuregulin-1 ,pc12 cells ,ferroptosis ,glutathione peroxidase ,oxygen-glucose deprivation/reoxygenation ,Medicine - Abstract
Objective To investigate the protective effect of neuregulin1β (NRG1β) on PC12 cell injury induced by oxygen-glucose deprivation/reoxygenation (OGD/R) and its mechanism. Methods OGD/R models of PC12 cells were established and randomly divided into control group (routinely cultured), model group (OGD/R-treated), and intervention group (treated with OGD/R and intervened by NRG1β). The cell viability of each group was measured by cell counting kit-8. The levels of reactive oxygen species (ROS) were measured by fluorescence intensity analysis. The levels of malondialdehyde (MDA) and the absorbance ratio of reduced glutathione/oxidized glutathione (GSH/GSSG) were measured by fluorescence spectrophotometry. Mitochondrial damage in each group was observed under a transmission electron microscope. The expression of glutathione peroxidase 4 (GPX4) was measured by Western blot. Results Compared with the control group, the model group had significantly decreased cell viability (t=25.76,P
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- 2023
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19. Small Structural Differences in Proline-Rich Decapeptides Have Specific Effects on Oxidative Stress-Induced Neurotoxicity and L-Arginine Generation by Arginosuccinate Synthase
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Carlos Alberto-Silva, Brenda Rufino da Silva, Julio Cezar Araujo da Silva, Felipe Assumpção da Cunha e Silva, Roberto Tadashi Kodama, Wilmar Dias da Silva, Maricilia Silva Costa, and Fernanda Calheta Vieira Portaro
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neuroprotective ,bioactive peptide ,proline-rich peptide ,snake venom ,oxidative stress ,PC12 cells ,Medicine ,Pharmacy and materia medica ,RS1-441 - Abstract
Introduction. The proline-rich decapeptide 10c (Bj-PRO-10c; ENWPHPQIPP) from the Bothrops jararaca snake modulates argininosuccinate synthetase (AsS) activity to stimulate L-arginine metabolite production and neuroprotection in the SH-SY5Y cell line. The relationships between structure, interactions with AsS, and neuroprotection are little known. We evaluated the neuroprotective effects of Bj-PRO-10c and three other PROs (Bn-PRO-10a, Bitis nasicornis snake venom, with a high degree of similarity to Bj-PRO-10c, on oxidative stress-induced toxicity in neuronal PC12 cells and L-arginine metabolite generation via AsS activity regulation. Methods. Cell integrity, metabolic activity, reactive oxygen species (ROS) production, and arginase activity were examined after 4 h of PRO pre-treatment and 20 h of H2O2-induced damage. Results. Only Bn-PRO-10a-MK and Bn-PRO-10c restored cell integrity and arginase function under oxidative stress settings, but they did not reduce ROS or cell metabolism. The MK dipeptide in Bn-PRO-10a-MK and valine (V8) in Bn-PRO-10c are important to these effects when compared to Bn-PRO-10a. Bj-PRO-10c is not neuroprotective in PC12 cells, perhaps because of their limited NMDA-type glutamate receptor activity. The PROs interaction analysis on AsS activation can be rated as follows: Bj-PRO-10c > Bn-PRO-10c > Bn-PRO-10a-MK > Bn-PRO-10a. The structure of PROs and their correlations with enzyme activity revealed that histidine (H5) and glutamine (Q7) in Bj-PRO-10c potentiated their affinity for AsS. Conclusions. Our investigation provides the first insights into the structure and molecular interactions of PROs with AsS, which could possibly further their neuropharmacological applications.
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- 2024
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20. Comparative Study of the Protective and Neurotrophic Effects of Neuronal and Glial Progenitor Cells-Derived Conditioned Media in a Model of Glutamate Toxicity In Vitro.
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Leonov, Georgy, Salikhova, Diana, Shedenkova, Margarita, Bukharova, Tatiana, Fatkhudinov, Timur, and Goldshtein, Dmitry
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PROGENITOR cells , *NEUROGLIA , *CELL morphology , *GLUTAMIC acid , *STEM cells - Abstract
Cell therapy represents a promising approach to the treatment of neurological diseases, offering potential benefits not only by cell replacement but also through paracrine secretory activities. However, this approach includes a number of limiting factors, primarily related to safety. The use of conditioned stem cell media can serve as an equivalent to cell therapy while avoiding its disadvantages. The present study was a comparative investigation of the antioxidant, neuroprotective and neurotrophic effects of conditioned media obtained from neuronal and glial progenitor cells (NPC-CM and GPC-CM) on the PC12 cell line in vitro. Neuronal and glial progenitor cells were obtained from iPSCs by directed differentiation using small molecules. GPC-CM reduced apoptosis, ROS levels and increased viability, expressions of the antioxidant response genes HMOX1 and NFE2L2 in a model of glutamate-induced oxidative stress. The neurotrophic effect was evidenced by a change in the morphology of pheochromocytoma cells to a neuron-like phenotype. Moreover, neurite outgrowth, expression of GAP43, TUBB3, MAP2, SYN1 genes and increased levels of the corresponding MAP2 and TUBB3 proteins. Treatment with NPC-CM showed moderate antiapoptotic effects and improved cell viability. This study demonstrated the potential application of CM in the field of regenerative medicine. [ABSTRACT FROM AUTHOR]
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- 2023
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21. 依托咪酯通过促进 miR-142-3p 表达减轻缺氧诱导的 PC12 细胞神经炎症反应 和细胞凋亡的分子机制研究.
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沈 磊, 李明霞, 彭 湃, 周军格, and 杨 军
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Objective: To investigate whether etomidate affects inflammatory response and apoptosis of PC12 cells induced by hypoxia by regulating miR-142-3p. Methods: PC12 cells were pretreated with different doses (2, 6, 12 µmol/L) of etomidate to establish hypoxia model; PC12 cells that transfected with miR-142-3p mimics or inhibitors were pretreated with 0 or 12 µmol/L of etomidate to establish hypoxia model. Cell viability, apoptosis and protein (CyclinD1, Cleaved-caspase-3) expressions were detected by CCK-8 method, flow cytometry and Western blot, respectively. ELISA was used to detect levels of inflammatory factors TNF-α, IL-1β, IL-6. Expression of miR-142-3p was detected by RT-qPCR. Results: Etomidate increased hypoxia-induced PC12 cells activity and expression of CyclinD1 protein and miR-142-3p, while decreased cell apoptosis rate, Cleaved-caspase-3 protein expression and levels of inflammatory factors TNF-α, IL-1β, IL-6 (P<0.05). Up-regulation of miR-142-3p increased activity and expression of CyclinD1 protein of hypoxia-induced PC12 cells, while decreased cell apoptosis rate, Cleaved-caspase-3 protein expression and levels of inflammatory factors TNF-α, IL-1β, IL-6 (P<0.05). Down-regulation of miR-142-3p reversed effects of etomidate on hypoxia-induced PC12 cell activity, apoptosis and expressions of inflammatory factors (P<0.05). Conclusion: Etomidate can reduce inflammatory response and apoptosis of PC12 cells induced by hypoxia, and its mechanism may be related to the up-regulation of miR-142-3p expression in cells. [ABSTRACT FROM AUTHOR]
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- 2023
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22. Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering.
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Olguín, Yusser, Selva, Mónica, Benavente, Diego, Orellana, Nicole, Montenegro, Ivan, Madrid, Alejandro, Jaramillo-Pinto, Diego, Otero, María Carolina, Corrales, Tomas P., and Acevedo, Cristian A.
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NERVE tissue , *PERIPHERAL nervous system , *ELECTRIC stimulation , *TISSUE engineering , *NEURAL stimulation , *BIOMATERIALS - Abstract
Extensive damage to peripheral nerves is a health problem with few therapeutic alternatives. In this context, the development of tissue engineering seeks to obtain materials that can help recreate environments conducive to cellular development and functional repair of peripheral nerves. Different hydrogels have been studied and presented as alternatives for future treatments to emulate the morphological characteristics of nerves. Along with this, other research proposes the need to incorporate electrical stimuli into treatments as agents that promote cell growth and differentiation; however, no precedent correlates the simultaneous effects of the types of hydrogel and electrical stimuli. This research evaluates the neural differentiation of PC12 cells, relating the effect of collagen, alginate, GelMA, and PEGDA hydrogels with electrical stimulation modulated in four different ways. Our results show significant correlations for different cultivation conditions. Electrical stimuli significantly increase neural differentiation for specific experimental conditions dependent on electrical frequency, not voltage. These backgrounds allow new material treatment schemes to be formulated through electrical stimulation in peripheral nerve tissue engineering. [ABSTRACT FROM AUTHOR]
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- 2023
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23. Total alkaloids from the seed embryo of Nelumbo nucifera Gaertn. improve cognitive impairment in APP/PS1 mice and protect Aβ-damaged PC12 cells.
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Meng, Xue-Lian, Xue, Jing-Su, Su, Shu-Jie, Gou, Jiang-Min, Lu, Jing, Chen, Chang-Lan, and Xu, Cheng-Bin
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EAST Indian lotus , *COGNITION disorders , *TRANSGENIC mice , *ALZHEIMER'S disease , *CHINESE medicine - Abstract
The seed embryo of Nelumbo nucifera Gaertn. is a famous traditional Chinese medicine and food which is considered conducive to the prevention of Alzheimer's disease (AD). In this study, the effect and mechanism of TASENN (total alkaloids from the seed embryo of Nelumbo nucifera Gaertn.) on AD mice and amyloid-β (Aβ) injured PC12 cells were evaluated. HPLC-UV analysis showed that the extracted TASENN (purity = 95.6%) mainly contains Liensinine, Isoliensinine, and Neferine (purity was 23.01, 28.02, and 44.57%, respectively). In vivo, oral treatment with TASENN (50 mg/kg/day for 28 days) improved the learning and memory functions of APP/PS1 transgenic mice, ameliorated the histopathological changes of cortical and hippocampal neurons, and inhibited neuronal apoptosis. We found that TASENN reduced the phosphorylation of Tau and the formation of neurofibrillary tangles (NFTs) in APP/PS1 mouse brain. Moreover, TASENN down-regulated the expression of APP and BACE1, ameliorated Aβ deposition, and inhibited microglial proliferation and aggregation. The elevated protein expression of CaM and p-CaMKII in APP/PS1 mouse brain was also reduced by TASENN. In vitro, TASENN inhibited the apoptosis of PC12 cells injured by Aβ25–35 and increased the cell viability. Aβ25–35-induced increase of cytosolic free Ca2+ level and high expression of CaM, p-CaMKII, and p-Tau were decreased by TASENN. Our findings indicate that TASENN has a potential therapeutic effect on AD mice and a protective effect on PC12 cells. The anti-AD activity of TASENN may be closely related to its negative regulation of the CaM pathway. [ABSTRACT FROM AUTHOR]
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- 2023
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24. Ferulic Acid Activates SIRT1-Mediated Ferroptosis Signaling Pathway to Improve Cognition Dysfunction in Wilson's Disease.
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Wang, Xie, Shao, Nan, Zhang, Xiaoyan, Chen, Hong, Chang, Ze, Xie, Daojun, and Zhang, Juan
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HEPATOLENTICULAR degeneration , *FERULIC acid , *CHINESE medicine , *CELLULAR signal transduction , *APOPTOSIS , *MITOCHONDRIAL pathology - Abstract
Background: Wilson's disease (WD), an autosomal recessive genetic disease, is characterized by copper metabolism disorder. WD patients may have a series of cognitive deficits in terms of neurological symptoms. Ferroptosis (FPT), a type of programmed cell death, is involved in the pathological progression of various cognitive disorders, and silent information regulator 1 (SIRT1) is considered to be a key factor in FPT. Ferulic acid (FA) is a traditional Chinese medicine monomer, with a remarkable effect in the clinical treatment of cognitive impairment-related disease. However, its intrinsic effect on FPT is still unclear. This study aims to investigate the protective effect of FA on cognitive impairment in animal and cell models of WD, and whether the pharmacological mechanism is related to the SIRT1-mediated FPT signaling pathway.Methods: Copper-loaded WD rats and PC12 cells WD were used as models of cognitive dysfunction in vivo and in vitro, respectively. Morris Water Maze (MWM) was used to evaluate the spatial exploration and memory abilities of rats. HE staining was used to observe neuronal damage in the CA1 region of the rat hippocampus. Immunofluorescence (IF) was used to detect the expression of GPX4 protein. Transmission electron microscopy (TEM) was used to observe the ultrastructure of neurons. The levels of Fe2+, MDA, SOD, GSH, 4HNE, and ROS were detected. Western blot and qRT-PCR were used to detect the protein and mRNA levels of SIRT1, Nrf2, SCL7A11, and GPX4.Results: In the WD copper-loaded model rats, MWM, TEM, and IF results showed that FA could promote the repair of learning and memory function, improve the morphological damage to hippocampal neurons, and maintain mitochondria integrity. In the PC12 cell experiment, the MTT method showed that FA increased the viability of copper-overloaded cell models. Western blot and qRT-PCR results confirmed that FA significantly increased the expression of proteins and mRNA in SIRT1, Nrf2, SCL7A11, and GPX4. In addition, FA reversed the expression of oxidative stress-related indicators, including MDA, SOD, GSH, 4HNE, and ROS.Conclusion: FA alleviates hippocampal neuronal injury by activating SIRT1-mediated FPT, providing a valuable candidate for traditional Chinese medicine monomer for the clinical therapeutics of WD cognitive impairment. [ABSTRACT FROM AUTHOR]
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- 2023
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25. Hyperoside Prevents Aβ42-Induced Neurotoxicity in PC12 Cells and Caenorhabditis elegans.
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Wang, Kexin, Zhang, Xinyue, Zhang, Miaosi, Li, Xin, Xie, Jiao, Liu, Suwen, Huang, Qun, Wang, Jilite, Guo, Qingbin, and Wang, Hao
- Abstract
Traditional Chinese medicines such as hyperoside-rich Acanthopanax senticosus and Crataegus pinnatifida have been confirmed to exhibit anti-oxidative stress properties. Hyperoside, the main ingredient of numerous antioxidant herbs, may have the ability to postpone the onset of neurodegenerative diseases. This study investigates the possible therapeutic mechanism of hyperoside as a natural antioxidant against Alzheimer's disease (AD) in Caenorhabditis elegans and PC12 cells. Specifically, hyperoside reduced reactive oxygen species (ROS) level and Aβ42-induced neurotoxicity in C. elegans worms. Meanwhile, hyperoside reduced ROS production and increased mitochondrial membrane potentialin Aβ42-induced PC12 cells, which possibly due to the increase of antioxidant enzymes activity and the diminution of malondialdehyde levels. Hoechst 33,342 staining and flow cytometry analysis results suggested that hyperoside reverses cell apoptosis. Network pharmacology predicts potentially relevant hyperoside targets and pathways in AD therapy. As anticipated, hyperoside reversed Aβ42-stimulated downregulation of the PI3K/Akt/Nrf2/HO-1. The PI3K inhibitor LY294002 partially abolished the protective capability of hyperoside. The results of molecular docking further indicated that the PI3K/Akt pathways may be involved in the protection of Aβ42-induced PC12 cells by hyperoside treatment. The study provides theoretical information for research and development of hyperoside as an antioxidant dietary supplement. [ABSTRACT FROM AUTHOR]
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- 2023
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26. Quassinoids from Twigs of Harrisonia perforata (Blanco) Merr and Their Anti-Parkinson's Disease Effect.
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Cai, Min, Bai, Xiao-Lin, Zang, Hao-Jing, Tang, Xiao-Han, Yan, Ying, Wan, Jia-Jia, Peng, Min-You, Liang, Hong, Liu, Lin, Guo, Feng, Zhao, Pei-Ji, Liao, Xun, Di, Ying-Tong, and Hao, Xiao-Jiang
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TWIGS , *BAEYER-Villiger rearrangement , *DOPAMINE receptors , *PARKINSON'S disease - Abstract
Six new C-20 and one new C-19 quassinoids, named perforalactones F-L (1–7), were isolated from twigs of Harrisonia perforata. Spectroscopic and X-ray crystallographic experiments were conducted to identify their structures. Through oxidative degradation of perforalactone B to perforaqussin A, the biogenetic process from C-25 quassinoid to C-20 via Baeyer–Villiger oxidation was proposed. Furthermore, the study evaluated the anti-Parkinson's disease potential of these C-20 quassinoids for the first time on 6-OHDA-induced PC12 cells and a Drosophila Parkinson's disease model of PINK1B9. Perforalactones G and I (2 and 4) showed a 10–15% increase in cell viability of the model cells at 50 μM, while compounds 2 and 4 (100 μM) significantly improved the climbing ability of PINK1B9 flies and increased the dopamine level in the brains and ATP content in the thoraces of the flies. [ABSTRACT FROM AUTHOR]
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- 2023
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27. Pioglitazone protects PC12 cells against oxidative stress injury: An in vitro study of its antiapoptotic effects via the PPAR? pathway.
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YALI LI, JUN LONG, LIBO LI, ZIYAO YU, YANJING LIANG, BIN HOU, LI XIANG, and XIAOLIN NIU
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OXIDATIVE stress , *REVERSE transcriptase polymerase chain reaction , *PEROXISOME proliferator-activated receptors , *PIOGLITAZONE , *SMALL interfering RNA - Abstract
To the best of our knowledge, the role of peroxisome proliferator-activated receptor γ (PPARγ) in oxidative stress-induced PC12 cell damage is unknown. Using a PC12 cell model with H2O2 treatment, the present study investigated the expression levels of apoptosis-related genes and neuronal apoptosis after oxidative stress injury. The present study further investigated the protective effect and mechanism of pioglitazone, a PPARγ agonist. PC12 cells treated with H2O2 were used as a model of oxidative stress injury. An MTT assay and flow cytometry were used to detect the effect of H2O2 on PC12 cell viability and the protective effect of pioglitazone. A TUNEL assay was used to detect neuronal apoptosis. The expression levels of PPARγ, Bax, Bcl-2 and caspase-3 were examined by reverse transcription-quantitative PCR and western blotting. H2O2 reduced PC12 cell viability in a dose- and time-dependent manner. H2O2 significantly upregulated the protein expression levels of Bax and the cleaved caspase-3/caspase-3 ratio (P<0.01), decreased the protein expression levels of Bcl-2 (P<0.01), and increased the apoptosis rate of PC12 cells. Pioglitazone significantly reduced the protein expression levels of Bax and the cleaved caspase-3/caspase-3 ratio (P<0.01), increased the expression levels of Bcl-2 (P<0.01), decreased the Bax/Bcl-2 expression ratio (P<0.01) and increased the viability of H2O2-damaged PC12 cells in a dose-dependent manner. Treatment with the PPARγ antagonist GW9662 or PPARγ small interfering RNA counteracted the protective effect of pioglitazone on PC12 cells to different extents (P<0.01). Therefore, the present study reported the role of PPARγ in protecting PC12 cells against oxidative stress injury, which may lead to novel therapeutic approaches for neurodegenerative diseases. [ABSTRACT FROM AUTHOR]
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- 2023
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28. SOX17过表达慢病毒载体和稳定转染细胞系的构建.
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黄少婷, 李友, 吴钊淳, 何嘉文, 廖科棋, and 李胜男
- Abstract
Objective To construct the sex-determined region Y-box 17 (SOX17) over-expression lentiviral vector, and to construct the cell line stably over-expressing SOX17 by using the PC12 cells infected with SOX17 over-expression lentivirus. Methods The over-expression sequence of SOX17 was designed and synthesized based on the National Center for Biotechnology Information (NCBI) Database, and was connected to the GV492 lentiviral vector after being doubly digested with BamHⅠ and AgeⅠ enzymes to construct the GV492-SOX17 over-expression recombinant plasmid. The agarose gel electrophoresis was used to identify the PCR products, and the positive bacteria carrying the GV492-SOX17 over-expression recombinant plasmid were selected, cloned and sequenced. The GV492 empty plasmid and GV492-SOX17 over-expression recombinant plasmid were transfected into the human embryonic kidney HEK 293T cells, respectively. After transfected for 48 h, the GV492 control lentivirus and GV492-SOX17 over-expression lentivirus were packaged and the viral titer was detected. The PC12 cells were divided into blank group, GV492 control group, and GV492-SOX17 group. The cells in blank group was not treated, and the cells in GV492 control and GV492-SOX17 groups were infected with the corresponding lentivirus (multiplicity of infection = 100), and the PC12 cells successfully infected with lentivirus were selected with 10 mg·L-1 puromycin. The growth state and green fluorescence expression of the PC12 cells were observed under fluorescence microscope;real-time fluorescence quantitative PCR (RT-qPCR) method was used to detect the expression level of SOX17 mRNA in the PC12 cells in various groups;Western blotting method was used to detect the expression level of SOX17 protein in the PC12 cells in various groups. Results The gene fragment length of GV492-SOX17 over-expression recombinant plasmid was about 744 bp, and the sequence of GV492-SOX17 over-expression recombinant plasmid gene was identical to the designed and synthesized SOX17 over-expression sequence. The titers of GV492 control lentivirus and GV492-SOX17 over-expression lentivirus were both 2.5×108 TU·mL-1. The growth state of the PC12 cells in various groups was good and the green fluorescence was expressed. The RT-qPCR results showed that the expression level of SOX17 mRNA in the cells in GV492-SOX17 group was significantly higher than those in blank group and GV492 control group( P<0.01). The Western blotting results showed that there was a specific band appeared at a relative molecular mass of 44 000, suggesting the SOX17 protein was successfully expressed in the PC12 cells; compared with blank group and GV492 control group, the expression level of SOX17 protein in the cells in GV492-SOX17 group was increased ( P<0.05) . Conclusion The GV492-SOX17 lentiviral expression vector is successfully constructed and the PC12 cell line stably over-expressing GV492-SOX17 is established. [ABSTRACT FROM AUTHOR]
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- 2023
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29. Stem cells alleviate OGD/R mediated stress response in PC12 cells following a co-culture: modulation of the apoptotic cascade through BDNF-TrkB signaling.
- Author
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Kaur, Harpreet, Sarmah, Deepaneeta, Datta, Aishika, Borah, Anupom, Yavagal, Dileep R., and Bhattacharya, Pallab
- Abstract
Apoptosis mediated by endoplasmic reticulum (ER) stress plays a crucial role in several neurovascular disorders, including ischemia/reperfusion injury (I/R injury). Previous in vitro and in vivo studies have suggested that following I/R injury, ER stress is vital for mediating CCAT-enhancer-binding protein homologous protein (CHOP) and caspase-12-dependent apoptosis. However, its modulation in the presence of stem cells and the underlying mechanism of cytoprotection remains elusive. In vivo studies from our lab have reported that post-stroke endovascular administration of stem cells renders neuroprotection and regulates apoptosis mediated by ER stress. In the current study, a more robust in vitro validation has been undertaken to decipher the mechanism of stem cell-mediated cytoprotection. Results from our study have shown that oxygen–glucose deprivation/reoxygenation (OGD/R) potentiated ER stress and apoptosis in the pheochromocytoma 12 (PC12) cell line as evident by the increase of protein kinase R (PKR)-like ER kinase (p-PERK), p-Eukaryotic initiation factor 2α subunit (EIF2α), activation transcription factor 4 (ATF4), CHOP, and caspase 12 expressions. Following the co-culture of PC12 cells with MSCs, ER stress was significantly reduced, possibly via modulating the brain-derived neurotrophic factor (BDNF) signaling. Furthermore, inhibition of BDNF by inhibitor K252a abolished the protective effects of BDNF secreted by MSCs following OGD/R. Our study suggests that inhibition of ER stress-associated apoptotic pathway with MSCs co-culture following OGD/R may help to alleviate cellular injury and further substantiate the use of stem cells as a therapeutic modality toward neuroprotection following hypoxic injury or stroke in clinical settings. [ABSTRACT FROM AUTHOR]
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- 2023
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30. The protective effect of apigenin against inorganic arsenic salt-induced toxicity in PC12 cells.
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Almeer, Rafa and Alyami, Nouf M.
- Subjects
NUCLEAR factor E2 related factor ,APIGENIN ,ARSENIC poisoning ,ARSENIC compounds ,POISONS - Abstract
Poisoning by arsenic affects people worldwide, and many human illnesses and health issues, including neurotoxicity, have been linked to chronic exposure to arsenic. When exposed to arsenic, the body produces intracellular reactive oxygen species (ROS), which influence a variety of alterations in cellular activity and directly harm molecules through oxidation. Arsenic-induced lesions are improved by antioxidants with the ability to lower ROS levels. Therefore, the current research aimed to assess how well apigenin protected PC12 cells from the toxicity caused by inorganic arsenic salt (iAs). For 24 and 48 h, iAs and/or apigenin were applied to PC12 cells. Then, oxidative stress indicators like malondialdehyde (MDA), nitric oxide (NO), and ROS in addition to the enzymatic and non-enzymatic antioxidant molecules such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD) were assessed. Moreover, after exposure to iAs, PC12 was examined for nuclear factor erythroid 2–related factor 2 (Nrf2) expression to clarify how apigenin manifests its neuroprotection. Furthermore, NF-kB p65 concentration and IL-1B, IL-6, and TNF-α mRNA expression were measured to assess neuroinflammation. Bax, caspase-3, and Bcl-2 levels were measured to investigate apigenin's potential to protect PC12 cells from iAs poisoning. The obtained results revealed that, the cell survival rate in the iAs group was significantly lower (P < 0.05), and the number of viable cells steadily increased after apigenin treatment. Furthermore, the study found that iAs decreased GSH, CAT, and SOD in the PC12 cells while increasing ROS, MDA, and NO levels. In PC12 cells, the capacity of iAs to cause oxidative stress was linked to the induction of neuroinflammation and apoptosis. Interestingly, apigenin pre-treatment of PC12 cells resulted in exceptional protection against iAs-induced neuroinflammation, oxidative stress, and apoptotic cell death. Nrf2 upregulation in PC12 cells may explain the neuroprotection effect of apigenin against iAs toxicity. In conclusion, the obtained results of the present study have clinical significance and indicate that apigenin is a promising candidate for shielding the nervous system from toxic effects caused by arsenic. These findings require further investigation using in vivo experimental models. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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31. Berberine Alleviates the Damage, Oxidative Stress and Mitochondrial Dysfunction of PC12 Cells Induced by High Glucose by Activating the KEAP1/Nrf2/ARE Pathway.
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Yuan, Haoyu, Wang, Baohua, Ye, Zicheng, and Li, Saimei
- Abstract
Diabetic encephalopathy (DE) is one of the major chronic complications of diabetes mellitus. This study aims to investigate the inhibitory effect of berberine (BBR) on the damage of PC12 cells induced by high glucose (HG). Differentiated PC12 cells were treated with different concentrations of glucose/BBR. The cell morphology, cell viability, lactate dehydrogenase (LDH) activity, apoptosis, oxidative stress (OS), mitochondrial structure, mitochondrial membrane potential (MMP), mitochondrial complex I-V activity, and adenosine triphosphate (ATP) levels were evaluated. The mRNA and protein levels of the Keap1/Nrf2/ARE pathway-related genes were assessed by RT-qPCR and Western blot. High-dose BBR and HG jointly treated-PC12 cells were treated with Nrf2-specific inhibitor ML385 to further verify whether Nrf2 was the target of BBR. The results showed that BBR inhibited cell damage, OS, and mitochondrial dysfunction induced by HG. The inhibitory effect of high BBR was more significant. The Keap1/Nrf2/ARE pathway was inhibited in PC12 cells induced by HG. BBR could activate the Keap1/Nrf2/ARE pathway, thus up-regulating the expression levels of antioxidant enzymes. ML385 antagonized the ameliorating effect of BBR on OS and mitochondrial dysfunction. The conclusion is that BBR can activate the Keap1/Nrf2/ARE pathway, upregulate the expression patterns of antioxidant enzymes, and reduce cell damage, OS, and mitochondrial dysfunction of PC12 cells induced by HG. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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32. SIP30 involvement in vesicle exocytosis from PC12 cells
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Ning Guo and Lei Yu
- Subjects
SIP30 ,Exocytosis ,Synaptic vesicles ,PC12 cells ,Neuropathic pain ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
SNAP25 (synaptosome-associated protein of 25 kDa) is a core SNARE (soluble N-ethylmaleimide-sensitive factor attachment receptor) protein; and the interaction between SNAP25 and other SNARE proteins is essential for synaptic vesicle exocytosis. Identified as a SNAP25 interacting protein, SIP30 (SNAP25 interacting protein at 30 kDa) has been shown to modulate neuropathic pain behavior, and is potentially involved in the cellular process of vesicle exocytosis. Previous study demonstrated that using a vesicle secretion assay in PC12 cells, anti-SIP30 siRNA reduced vesicle exocytosis. We investigated vesicle exocytosis from PC12 cells with FM1-43 fluorescence dye, and demonstrated that anti-SIP30 siRNA reduced the pool of releasable vesicles and the rate of vesicle exocytosis, without affecting the endocytosis and recycling of the exocytosed vesicles. The results show that SIP30 is involved in vesicle exocytosis, suggesting a potential mechanism of SIP30 modulation of neuropathic pain.
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- 2024
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33. α1‐Antitrypsin derived SP16 peptide demonstrates efficacy in rodent models of acute and neuropathic pain
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Wang, Zixuan, Martellucci, Stefano, Van Enoo, Alicia, Austin, Dana, Gelber, Cohava, and Campana, Wendy M
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Biomedical and Clinical Sciences ,Neurosciences ,Clinical Sciences ,Neurodegenerative ,Pain Research ,Peripheral Neuropathy ,Chronic Pain ,Aetiology ,2.1 Biological and endogenous factors ,Acute Pain ,Animals ,Disease Models ,Animal ,Low Density Lipoprotein Receptor-Related Protein-1 ,MAP Kinase Signaling System ,Male ,Mice ,Neuralgia ,Neurites ,PC12 Cells ,Peptides ,Rats ,Rats ,Sprague-Dawley ,Schwann Cells ,Sensory Receptor Cells ,alpha 1-Antitrypsin ,LRP1 ,neuroinflammation ,neuropathic pain ,nociception ,peripheral nerve injury ,SP16 ,Biochemistry and Cell Biology ,Physiology ,Medical Physiology ,Biochemistry & Molecular Biology ,Biochemistry and cell biology ,Medical physiology - Abstract
SP16 is an innovative peptide derived from the carboxyl-terminus of α1-Antitrypsin (AAT), corresponding to residues 364-380, and contains recognition sequences for the low-density lipoprotein receptor-related protein-1 (LRP1). LRP1 is an endocytic and cell-signaling receptor that regulates inflammation. Deletion of Lrp1 in Schwann cells increases neuropathic pain; however, the role of LRP1 activation in nociceptive and neuropathic pain regulation remains unknown. Herein, we show that SP16 is bioactive in sensory neurons in vitro. Neurite length and regenerative gene expression were increased by SP16. In PC12 cells, SP16 activated Akt and ERK1/2 cell-signaling in an LRP1-dependent manner. When formalin was injected into mouse hind paws, to model inflammatory pain, SP16 dose-dependently attenuated nociceptive pain behaviors in the early and late phases. In a second model of acute pain using capsaicin, SP16 significantly reduced paw licking in both male and female mice (p
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- 2022
34. Analyzing Angiotensin II Receptor Type 1 Clustering in PC12 Cells in Response to Hypoxia Using Direct Stochastic Optical Reconstruction Microscopy (dSTORM)
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Aldossary, Hayyaf S., Nieves, Daniel J., Kavanagh, Deirdre M., Owen, Dylan, Ray, Clare J., Kumar, Prem, Coney, Andrew M., Holmes, Andrew P., Crusio, Wim E., Series Editor, Dong, Haidong, Series Editor, Radeke, Heinfried H., Series Editor, Rezaei, Nima, Series Editor, Steinlein, Ortrud, Series Editor, Xiao, Junjie, Series Editor, Conde, Sílvia V., editor, Iturriaga, Rodrigo, editor, del Rio, Rodrigo, editor, Gauda, Estelle, editor, and Monteiro, Emília C., editor
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- 2023
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35. Investigating the effect of telmisartan on acrylamide-induced neurotoxicity through in vitro and in vivo methods
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Zahra Yazadanpanah, Mahboobeh Ghasemzadeh Rahbardar, Bibi Marjan Razavi, and Hossein Hosseinzadeh
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anti-oxidants ,cell survival ,motor disorders ,neurotoxins ,pc12 cells ,vitamin e ,Medicine - Abstract
Objective(s): Acrylamide (ACR) is an environmental contaminant and neurotoxin. Telmisartan is an AT1 blocker that has neuroprotective properties basically through its anti-oxidant effect. The effect of telmisartan on ACR-induced neurotoxicity was investigated in this study. Materials and Methods: Male Wistar rats were randomly assigned to eight groups (n=6): 1:Control (normal saline), 2:ACR (50 mg/kg, 11 days, IP), 3:ACR+vitamin E (200 mg/kg, every other day, 11 days), 4-6:ACR+telmisartan (0.6, 1.25, and 2.5 mg/kg, 11 days, IP), 7:ACR+telmisartan (0.6 mg/kg, days 3–11), 8:Telmisartan (2.5 mg/kg, 11 days). The behavioral test and blood pressure were assessed after 11 days. Then, the levels of MDA and GSH in brain tissue were measured. The MTT assay was used to evaluate the effect of telmisartan on ACR-induced cytotoxicity.Results: Exposing PC12 cells to ACR decreased cell viability versus the control group. Pretreating PC12 cells with telmisartan (0.0125, 0.025 µM) enhanced cell viability compared with the ACR group. Compared with control samples, ACR significantly caused motor impairment, elevated MDA, and reduced GSH levels. Locomotor abnormalities were significantly ameliorated by telmisartan (0.6, 1.25 mg/kg, 11 days) and vitamin E versus the ACR group. Receiving telmisartan (0.6, 1.25, and 2.5 mg/kg) and vitamin E along with ACR decreased MDA levels and enhanced GSH content compared with the ACR group. There was no significant difference in animal blood pressure between the groups.Conclusion: Oxidative stress has a chief role in the neurotoxicity of ACR. Telmisartan (in doses that do not affect blood pressure) ameliorated ACR-induced toxicity by inhibiting oxidative stress.
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- 2023
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36. Protection of Si Nanowires against Aβ Toxicity by the Inhibition of Aβ Aggregation
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Xuechun Zhao, Chenye Mou, Jiayi Xu, Wei Cui, Yijing Shi, Yangzhe Wang, Tian Luo, Wei Guo, Jichun Ye, and Wanghua Chen
- Subjects
Alzheimer’s disease ,amyloid β ,silicon nanowires ,PC12 cells ,toxicity ,Organic chemistry ,QD241-441 - Abstract
Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by the accumulation of amyloid beta (Aβ) plaques in the brain. Aβ1–42 is the main component of Aβ plaque, which is toxic to neuronal cells. Si nanowires (Si NWs) have the advantages of small particle size, high specific surface area, and good biocompatibility, and have potential application prospects in suppressing Aβ aggregation. In this study, we employed the vapor–liquid–solid (VLS) growth mechanism to grow Si NWs using Au nanoparticles as catalysts in a plasma-enhanced chemical vapor deposition (PECVD) system. Subsequently, these Si NWs were transferred to a phosphoric acid buffer solution (PBS). We found that Si NWs significantly reduced cell death in PC12 cells (rat adrenal pheochromocytoma cells) induced by Aβ1–42 oligomers via double staining with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and fluorescein diacetate/propyl iodide (FDA/PI). Most importantly, pre-incubated Si NWs largely prevented Aβ1–42 oligomer-induced PC12 cell death, suggesting that Si NWs exerts an anti-Aβ neuroprotective effect by inhibiting Aβ aggregation. The analysis of Fourier Transform Infrared (FTIR) results demonstrates that Si NWs reduce the toxicity of fibrils and oligomers by intervening in the formation of β-sheet structures, thereby protecting the viability of nerve cells. Our findings suggest that Si NWs may be a potential therapeutic agent for AD by protecting neuronal cells from the toxicity of Aβ1–42.
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- 2024
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37. Extracellular vesicles from neurons promote neural induction of stem cells through cyclin D1
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Song, Lu, Tian, Xinran, and Schekman, Randy
- Subjects
Stem Cell Research - Embryonic - Non-Human ,Stem Cell Research ,Neurosciences ,1.1 Normal biological development and functioning ,Underpinning research ,Neurological ,Animals ,Cell Communication ,Cell Differentiation ,Cell Line ,Cell Line ,Tumor ,Cyclin D1 ,Cyclin-Dependent Kinase 4 ,DNA-Binding Proteins ,Endosomal Sorting Complexes Required for Transport ,Extracellular Vesicles ,Gene Expression Regulation ,HSC70 Heat-Shock Proteins ,Membrane Proteins ,Mice ,Mouse Embryonic Stem Cells ,Nestin ,Neurogenesis ,Neurons ,PAX6 Transcription Factor ,PC12 Cells ,Rats ,Signal Transduction ,Transcription Factors ,Tretinoin ,Biological Sciences ,Medical and Health Sciences ,Developmental Biology - Abstract
Extracellular vesicles (EVs) are thought to mediate the transport of proteins and RNAs involved in intercellular communication. Here, we show dynamic changes in the buoyant density and abundance of EVs that are secreted by PC12 cells stimulated with nerve growth factor (NGF), N2A cells treated with retinoic acid to induce neural differentiation, and mouse embryonic stem cells (mESCs) differentiated into neuronal cells. EVs secreted from in vitro differentiated cells promote neural induction of mESCs. Cyclin D1 enriched within the EVs derived from differentiated neuronal cells contributes to this induction. EVs purified from cells overexpressing cyclin D1 are more potent in neural induction of mESC cells. Depletion of cyclin D1 from the EVs reduced the neural induction effect. Our results suggest that EVs regulate neural development through sorting of cyclin D1.
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- 2021
38. Antagonism of a key peptide 'T14' driving neurodegeneration: Evaluation of a next generation therapeutic
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Sanskar Ranglani, Sibah Hasan, Kashif Mahfooz, Jack Gordon, Sara Garcia-Rates, and Susan Greenfield
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Alzheimer’s disease ,AChE peptide ,NBP6B ,PC12 cells ,NBP14 ,VSDI ,Therapeutics. Pharmacology ,RM1-950 - Abstract
T14, a 14mer peptide derived from the C-terminus of acetylcholinesterase (AChE) is a signalling molecule that could drive neurodegeneration via the alpha 7 nicotinic acetylcholine receptor. Its levels increase as Alzheimer’s pathology progresses; however, a cyclic variant of the compound, NBP14, can block the effects of the endogenous linear counterpart in-vitro, ex vivo, and in vivo. Here, we explore the antagonistic potential of two 6mer peptides, NBP6A and NBP6B. These are smaller linear versions of NBP14, designed to be more effective by modifying the amino acid residues to enhance receptor blockade alongside other relevant solubility parameters. The peptides were tested in-vitro in PC12 cells on three parameters, calcium influx, cell viability, and AChE release, and ex vivo using voltage sensitive dye imaging (VSDI) in rat brain slices. Neither NBP6A nor NBP6B applied alone had any effect. In PC12 cells, NBP6B was identified as the more potent molecule since it demonstrated more effective blockade of T14 action on calcium influx, cell viability, and AChE release. NBP6B was then further evaluated using VSDI, where it proved twice as potent as NBP14 in blocking the action of T14. The improved effect of NBP6B in blocking the actions of T14, combined with its smaller size suggests that this variant could have even greater therapeutic potential than its original cyclic compound, for treating neurodegenerative disorders.
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- 2023
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39. Evaluating the effect of alpha-mangostin on neural toxicity induced by acrylamide in rats.
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Ghobakhlou, Farivar, Eisvand, Farhad, Razavi, Bibi Marjan, Ghasemzadeh Rahbardar, Mahboobeh, and Hosseinzadeh, Hossein
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ACRYLAMIDE ,BAX protein ,VITAMIN E ,NEUROTOXIC agents ,REACTIVE oxygen species ,MOVEMENT disorders - Abstract
Acrylamide (ACR) is known to be a neurotoxic agent for humans and animals that has many applications in industry. Alpha-mangostin is a natural antioxidant that is extracted from mangosteen. This study aimed to investigate the protective effects of alpha-mangostin against ACR-induced neurotoxicity in rats and PC12 cells. Male Wistar rats were used in this investigation for 11 days, divided into 8 groups: 1. control group (normal saline), 2. ACR (50 mg/kg, i.p.), 3–6. ACR + alpha-mangostin (20, 40, 60 mg/kg, p.o.), 7. ACR + vitamin E (200 mg/kg, i.p., every other day) 8. alpha-mangostin (60 mg/kg, p.o.). On the last day of the study, the behavioral test was performed. The amounts of malondialdehyde (MDA) and glutathione (GSH) were measured. Also, the effects of ACR and alpha-mangostin were assessed by MTT assay on PC12 cells, and the levels of reactive oxygen species (ROS), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and cleaved caspase-3 proteins were measured by Western blotting. Receiving ACR caused motor disorders in animals, increased MDA, and decreased GSH levels of the cerebral cortex versus the control group. Alpha-mangostin (60 mg/kg) reduced ACR motility disorders, MDA amounts, and augmented GSH levels. The concurrent administration of vitamin E and ACR reduced gait score, MDA level, and amplified GSH content versus the ACR group. In the in vitro section, alpha-mangostin (1.25 µM, 24 h) increased cell viability, attenuated ROS, Bax/Bcl-2, and cleaved caspase-3 levels versus the ACR group. Alpha-mangostin reduced the toxicity of ACR by inhibiting oxidative stress and apoptosis. Therefore, it could be a promising compound for managing ACR-induced neurotoxicity. [ABSTRACT FROM AUTHOR]
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- 2023
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40. Investigating the effect of telmisartan on acrylamide-induced neurotoxicity through in vitro and in vivo methods.
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Yazdanpanah, Zahra, Rahbardar, Mahboobeh Ghasemzadeh, Razavi, Bibi Marjan, and Hosseinzadeh, Hossein
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TELMISARTAN , *NEUROTOXICOLOGY , *VITAMIN E , *PRESSURE groups , *BLOOD pressure - Abstract
Objective(s): Acrylamide (ACR) is an environmental contaminant and neurotoxin. Telmisartan is an AT1 blocker that has neuroprotective properties basically through its anti-oxidant effect. The effect of telmisartan on ACR-induced neurotoxicity was investigated in this study. Materials and Methods: Male Wistar rats were randomly assigned to eight groups (n=6): 1:Control (normal saline), 2:ACR (50 mg/kg, 11 days, IP), 3:ACR+vitamin E (200 mg/kg, every other day, 11 days), 4-6:ACR+telmisartan (0.6, 1.25, and 2.5 mg/kg, 11 days, IP), 7:ACR+telmisartan (0.6 mg/kg, days 3-11), 8:Telmisartan (2.5 mg/kg, 11 days). The behavioral test and blood pressure were assessed after 11 days. Then, the levels of MDA and GSH in brain tissue were measured. The MTT assay was used to evaluate the effect of telmisartan on ACR-induced cytotoxicity. Results: Exposing PC12 cells to ACR decreased cell viability versus the control group. Pretreating PC12 cells with telmisartan (0.0125, 0.025 µM) enhanced cell viability compared with the ACR group. Compared with control samples, ACR significantly caused motor impairment, elevated MDA, and reduced GSH levels. Locomotor abnormalities were significantly ameliorated by telmisartan (0.6, 1.25 mg/kg, 11 days) and vitamin E versus the ACR group. Receiving telmisartan (0.6, 1.25, and 2.5 mg/kg) and vitamin E along with ACR decreased MDA levels and enhanced GSH content compared with the ACR group. There was no significant difference in animal blood pressure between the groups. Conclusion: Oxidative stress has a chief role in the neurotoxicity of ACR. Telmisartan (in doses that do not affect blood pressure) ameliorated ACR-induced toxicity by inhibiting oxidative stress. [ABSTRACT FROM AUTHOR]
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- 2023
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41. Protective Effects of Safflor Yellow A Against H2O2-induced Oxidative Stress Injury in PC12 Cells via Nrf2/HO-1 Pathway.
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Chen, Hui, Gu, Sichun, You, Yi, Xie, Anjie, Lu, Yiying, and Wang, Changde
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- *
OXIDATIVE stress , *SAFFLOWER , *HEME oxygenase , *NEURONS , *WESTERN immunoblotting - Abstract
Background: Safflor yellow (SY) is a water-capacitive component of dried flowers, mainly from safflor in the safflower family, which contains a large amount of bruthin A, or Safflor yellow A (SYA) for short. Studies have reported the protective effects of SYA against oxidative stress injury in nerve cells. Materials and Methods: In this study, H2O2-treated PC12 cells were used as experimental materials to explore how SYA plays a protective role against nerve cells after oxidative stress and to analyze the molecular mechanism of SYA. PC12 cells were treated with H2O2 and SYA solution. Cell proliferation is then detected with Cell Counting Kit-8 (CCK-8). The detection method is 5-(and 6)-chloromethyl-2′,7′-dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) staining reactive oxygen species (ROS). Levels of apoptosis-related proteins, including Bcl2-associated X protein (Bax), B-cell lymphoma (Bcl-2), caspase-3, and cleaved caspase-3, and oxidative stress regulators, namely, nuclear factor erythroid-2-related actor 2 (Nrf2) and heme oxygenase 1 (HO-1), were detected by western blotting. Results: The CCK-8 assay results showed that the survival rate of H2O2-induced PC12 cells was significantly increased after SYA treatment. Fluorescence microscopy (CM-H2DCF-DA staining) indicated that SYA decreased H2O2-induced apoptosis in PC12 cells by reducing intracellular ROS. Western blotting analysis showed that SYA upregulated the expression of Nrf2, HO-1, and Bcl-2 and downregulated the expression of Bax, caspase 3, and cleaved caspase-3 in H2O2-induced PC12 cells. Conclusion: SYA does protect the nerve cells from oxidative stress damage. Its mechanism of action is mainly related to whether the Nrf2/HO-1 pathway is activated. [ABSTRACT FROM AUTHOR]
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- 2023
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42. The Temporal and Spatial Changes of Autophagy and PI3K Isoforms in Different Neural Cells After Hypoxia/Reoxygenation Injury.
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Zhang, Duo, Chen, Xuanyu, Liu, Baoge, Yuan, Yuan, Cui, Wei, Zhu, Di, Zhu, Jichao, Duan, Shuo, and Li, Chenxi
- Abstract
There are limited therapeutic options for patient with traumatic spinal cord injury (SCI). Phosphoinositide 3-kinase family (PI3Ks) are the key molecules for regulating cell autophagy, which is a possible way of treating SCI. As we know, PI3K family are composed of eight isoforms, which are distributed into three classes. While the role of PI3Ks in regulating autophagy is controversial and the effects may be in a cell-specific manner. Different isoforms do not distribute in neural cells consistently and it is not clear how the PI3K isoforms regulate and interact with autophagy. Therefore, we explored the distributions and expression of different PI3K isoforms in two key neural cells (PC12 cells and astrocytes). The results showed that the expression of LC3II/I and p62, which are the markers of autophagy, changed in different patterns in PC12 cells and astrocytes after hypoxia/reoxygenation injury (H/R). Furthermore, the mRNA level of eight PI3K isoforms did not change in the same way, and even for the same isoform the mRNA activities are different between PC12 cells and astrocytes. What is more, the results of western blot of PI3K isoforms after H/R were inconsistent with the relevant mRNA. Based on this study, the therapeutic effects of regulating autophagy on SCI are not confirmed definitely, and its molecular mechanisms may be related with different temporal and spatial patterns of activation and distributions of PI3K isoforms. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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43. Neuroprotective Iridoids and Lignans from Valeriana amurensis.
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Ye, Minhui, Lin, Xiaoju, Wang, Qiuhong, Yang, Bingyou, and Wang, Changfu
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IRIDOIDS , *LIGNANS , *VALERIANA , *NEUROPROTECTIVE agents , *LACTATE dehydrogenase - Abstract
Valeriana amurensis (V. amurensis) is widely distributed in Northeast China. In addition to medicines, it has also been used to prepare food, wine, tobacco, cosmetics, perfume, and functional foods. Other studies have investigated the neuroprotective effects of V. amurensis extract. As the therapeutic basis, the active constituents should be further evaluated. In this paper, six new compounds (1–6) were isolated, including five iridoids (Xiecaoiridoidside A–E) and one bisepoxylignan (Xiecaolignanside A), as well as six known compounds (7–12). The neuroprotective effects of 1–12 were also investigated with amyloid β protein 1−42 (Aβ1-42)-induced injury to rat pheochromocytoma (PC12) cells. As a result, iridoids 1 and 2 and lignans 6, 8, and 9 could markedly maintain the cells' viability by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) and lactate dehydrogenase (LDH) release assay. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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44. Doxycycline inhibits dopaminergic neurodegeneration through upregulation of axonal and synaptic proteins.
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do Amaral, Lilian, dos Santos, Neife Aparecida Guinaim, Sisti, Flávia Malvestio, Del Bel, Elaine, and dos Santos, Antônio Cardozo
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DOXYCYCLINE ,NERVE growth factor ,PARKINSON'S disease ,NEURODEGENERATION ,CHONDROITIN sulfate proteoglycan ,TUBULINS ,DRUG repositioning - Abstract
Doxycycline (DOX) is a widely used antibiotic that is able to cross the blood–brain barrier. Several studies have shown its neuroprotective effect against neurodegeneration and have associated it with antioxidant, anti-apoptotic, and anti-inflammatory mechanisms. We have recently demonstrated that DOX mimics nerve growth factor (NGF) signaling in PC12 cells. However, the involvement of this mechanism in the neuroprotective effect of DOX is unknown. Axonal degeneration and synaptic loss are key events at the early stages of neurodegeneration, and precede the neuronal death in neurodegenerative diseases, including Parkinson's disease (PD). Therefore, the regeneration of the axonal and synaptic network might be beneficial in PD. The effect of DOX in PC12 cells treated with the Parkinsonian neurotoxin 1-methyl-4-phenylpyridinium (MPP
+ ) was addressed. Doxycycline reduced the inhibition of neuritogenesis induced by MPP+ , even in cells deprived of NGF. The mechanism involved the upregulation of GAP-43, synapsin I, β-III-tubulin, F-actin, and neurofilament-200, proteins that are associated with axonal and synaptic plasticity. Considering the role of axonal degeneration and synaptic loss at the initial stages of PD, the recent advances in early diagnosis of neurodegeneration, and the advantages of drug repurposing, doxycycline is a promising candidate to treat PD. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
45. 木犀草苷对阿尔茨海默病模型细胞凋亡和炎性因子 表达的研究.
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王翠, 杨畅, 金玉, 高蜜, 张雯, 王琼, and 金海涛
- Abstract
Objective To explore the effect and possible mechanism of cynaroside on apoptosis and expression of inflammatory factor in model cells of Alzheimer's disease (AD) by down-regulating mitogen-activated protein kinase kinase kinase 3 (MEKK3). Methods PC12 cells were cultured in vitro and incubated with different doses (6.25 mg/L, 12.5 mg/L, 25 mg/L) of cynaroside for 24 h to establish an AD cell model induced by Aβ25-35. In addition, MEKK3 small interfering RNA or overexpression vector was transfected into PC12 cells, and Aβ25-35 induced AD cell model was established after incubation with 25 mg/L luteolin for 24 h. CCK-8 was used to detect inhibition rate of cell proliferation, and flow cytometry was used to detect apoptosis rate. ELISA was used to detect inflammatory factors (TNF- α, IL-6, IL-1β). The protein expression of MEKK3 was detected by Western blot assay. Results Cynaroside could decrease the proliferation inhibition rate, apoptosis rate, inflammatory cytokines (TNF-α, IL-6, IL-1β) and MEKK3 protein expression in PC12 cells induced by Aβ25-35 (P< 0.05). Knockdown of MEKK3 could reduce the proliferation inhibition rate, apoptosis rate and inflammatory cytokines (TNFα, IL-6, IL-1β) of Aβ25-35-induced PC12 cells, while overexpression of MEKK3 showed the opposite effect. Upregulation of MEKK3 expression attenuated effects of cynaroside on the proliferation inhibition rate, apoptosis rate and inflammatory factors of Aβ25-35-induced PC12 cells. Conclusion Cynaroside may inhibit apoptosis and expression of inflammatory factors of AD model cells by down-regulating MEKK3, which has potential value in the treatment of AD. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
46. Neuroprotective Effect of Cyclosporine Against Oxidative Stress-Induced Neurotoxicity in PC12 Cells
- Author
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Liravi, Azadeh, Mehrabani, Mehrnaz, Karami-Mohajeri, Somayyeh, and Aminzadeh, Azadeh
- Published
- 2024
- Full Text
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47. Integrating Network Pharmacology, Molecular Docking and Pharmacological Evaluation for Exploring the Polyrhachis vicina Rogers in Ameliorating Depression
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He J, Han D, Jia C, Xie J, Zhu F, Wei J, Li D, Wei D, Li Y, Tang L, Wei G, Yan J, Tong Y, Yang L, and Tan X
- Subjects
pc12 cells ,bdnf ,corticosterone ,camp signaling pathway ,gc-ms ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Junhui He,1,* Dongbo Han,2,* Chunlian Jia,2,* Jiaxiu Xie,1 Fucui Zhu,2 Jie Wei,1 Dongmei Li,1 Dongmei Wei,1 Yi Li,1 Li Tang,3 Guining Wei,1,4 Jing Yan,4 Yuanming Tong,1 Lifang Yang,4,* Xuecai Tan4,* 1Department of Pharmacology, Key Laboratory of Quality Standards, Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning, 530022, People’s Republic of China; 2Department of Pharmacology, Guangxi Medical University, Nanning, 530021, People’s Republic of China; 3Department of Pharmacy, the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, 530022, People’s Republic of China; 4Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning, 530008, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xuecai Tan; Lifang Yang, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning, 530008, People’s Republic of China, Tel +86 0771-5877473, Email gxunxctan@126.com; yanglf1990@163.comPurpose: To investigate the mechanisms of antidepressant action of active fraction of Polyrhachis vicina Rogers (AFPR) through network pharmacology, molecular docking and experimental validation.Methods: GC-MS was used to predict chemical compounds, corresponding databases were used to predict chemical compound targets and depression targets, Cytoscape software was used to construct and analyze the protein interaction network map, DAVID database was used to analyze gene ontology (GO) and KEGG signaling pathway, and AGFR software was used to perform molecular docking. Subsequently, the underlying action mechanisms of AFPR on depression predicted by network pharmacology analyses were experimentally validated in a CORT-induced depression model in vitro and in vivo.Results: A total of 52 potential targets of AFPR on antidepressant were obtained. GO is mainly related to chemical synaptic transmission, signal transduction and others. KEGG signaling pathways are mainly related to cAMP signaling pathway and C-type lectin receptor signaling pathway. The experiment results showed that AFPR significantly increased the expression of PRKACA, CREB and BDNF in mouse brain tissue and PC12 cells. Furthermore, after interfered of cAMP in PC12 cells, the decreased expression of PRKACA, CREB and BDNF was reversed by AFPR.Conclusion: AFPR may exert antidepressant effects through multiple components, targets and pathways. Furthermore, it could improve neuroplasticity via the cAMP signaling pathway to improve depression-like symptoms.Graphical Abstract: Keywords: PC12 cells, BDNF, corticosterone, CAMP signaling pathway, GC-MS
- Published
- 2023
48. Propofol attenuates hypoxia-induced inflammation and apoptosis in rat pheochromocytoma cell line PC12
- Author
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GU Wei, ZHU Jianping, WU Pinwen
- Subjects
pc12 cells ,inflammatory response ,oxidative stress ,apoptosis ,mir-141-3p ,Medicine - Abstract
Objective To investigate whether propofol inhibits the expression of miR-141-3p and reduces the molecular mechanism of hypoxia-induced inflammation and apoptosis of pheochromocytoma cell line PC12. Methods PC12 cells were divided into control group, hypoxia group, (5, 10, 20)μmol/L propofol+hypoxia group, anti-miR-con+hypoxia group, anti-miR-141-3p+hypoxia group, miR-con+20 μmol/L propofol+hypoxia group, miR-141-3p+20 μmol/L propofol+hypoxia group. Flow cytometry was used to detect the apoptosis of PC12 cells; Western blot was employed to determine the expression of activated cleaved caspase-3 (cleaved caspase-3) protein, and the kits were implemented to monitor malondialdehyde (MDA) content and superoxide dismutase (SOD) activity; Reactive oxygen species fluorescent probe DCFH-DA method to determine reactive oxygen species (ROS) content; ELISA kits to assay tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 content, RT-qPCR to detect the expression of miR-141-3p. Results Compared with the control group, the apoptosis rate, cleaved caspase-3 protein expression level, MDA, ROS, TNF-α, IL-1β, IL-6 content and miR-141-3p expression of PC12 cells in hypoxia group were all increased, while SOD activity weakened (PPPPConclusions Propofol can alleviate the inflammatory response, oxidative stress and apoptosis of PC12 cells induced by hypoxia by inhibiting the expression of miR-141-3p.
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- 2023
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49. Pheochromocytoma-paraganglioma type 5 syndrome associated with mutation in the succinate dehydrogenase type A complex (SDHA), a case report
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Myriam Vanessa Rueda-Galvis, Alejandro Román-Gonzaléz, and Valentina Agredo-Delgado
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pheochromocytoma ,pc12 cells ,paraganglioma ,succinate dehydrogenase ,neurosecretory systems ,Medicine ,Medicine (General) ,R5-920 - Abstract
Pheochromocytomas and paragangliomas are rare neuroendocrine neoplasms that can produce catecholamines, with an incidence of less than one case per million inhabitants. They are histologically benign and minimally different from their malignant counterparts. The most common mutation is in the succinate dehydrogenase (SDH) complex pathway, and one of the least reported is the SDH subunit A (SDHA) mutation. A case of a patient with pheochromocytoma and paraganglioma, associated with mutation in SDHA, its behavior and good clinical response to surgical management and active surveillance is described.
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- 2023
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50. Upregulation of RIN3 induces endosomal dysfunction in Alzheimer’s disease
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Shen, Ruinan, Zhao, Xiaobei, He, Lu, Ding, Yongbo, Xu, Wei, Lin, Suzhen, Fang, Savannah, Yang, Wanlin, Sung, Kijung, Spencer, Brian, Rissman, Robert A, Lei, Ming, Ding, Jianqing, and Wu, Chengbiao
- Subjects
Biological Psychology ,Biomedical and Clinical Sciences ,Neurosciences ,Psychology ,Brain Disorders ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Dementia ,Aging ,Alzheimer's Disease ,Acquired Cognitive Impairment ,Neurodegenerative ,Aetiology ,2.1 Biological and endogenous factors ,Neurological ,Alzheimer Disease ,Animals ,Brain ,Carrier Proteins ,Cells ,Cultured ,Endosomes ,Guanine Nucleotide Exchange Factors ,HEK293 Cells ,Humans ,Male ,Mice ,Mice ,Inbred C57BL ,Mice ,Transgenic ,PC12 Cells ,Protein Interaction Domains and Motifs ,Rats ,Up-Regulation ,Alzheimer's disease ,AD risk factors ,Trafficking ,RIN3 ,BIN1 ,CD2AP ,Tau ,Alzheimer’s disease ,Clinical Sciences ,Biological psychology - Abstract
BackgroundIn Alzheimer's Disease (AD), about one-third of the risk genes identified by GWAS encode proteins that function predominantly in the endocytic pathways. Among them, the Ras and Rab Interactor 3(RIN3) is a guanine nucleotide exchange factor (GEF) for the Rab5 small GTPase family and has been implicated to be a risk factor for both late onset AD (LOAD) and sporadic early onset AD (sEOAD). However, how RIN3 is linked to AD pathogenesis is currently undefined.MethodsQuantitative PCR and immunoblotting were used to measure the RIN3 expression level in mouse brain tissues and cultured basal forebrain cholinergic neuron (BFCNs). Immunostaining was used to define subcellular localization of RIN3 and to visualize endosomal changes in cultured primary BFCNs and PC12 cells. Recombinant flag-tagged RIN3 protein was purified from HEK293T cells and was used to define RIN3-interactomes by mass spectrometry. RIN3-interacting partners were validated by co-immunoprecipitation, immunofluorescence and yeast two hybrid assays. Live imaging of primary neurons was used to examine axonal transport of amyloid precursor protein (APP) and β-secretase 1 (BACE1). Immunoblotting was used to detect protein expression, processing of APP and phosphorylated forms of Tau.ResultsWe have shown that RIN3 mRNA level was significantly increased in the hippocampus and cortex of APP/PS1 mouse brain. Basal forebrain cholinergic neurons (BFCNs) cultured from E18 APP/PS1 mouse embryos also showed increased RIN3 expression accompanied by early endosome enlargement. In addition, via its proline rich domain, RIN3 recruited BIN1(bridging integrator 1) and CD2AP (CD2 associated protein), two other AD risk factors, to early endosomes. Interestingly, overexpression of RIN3 or CD2AP promoted APP cleavage to increase its carboxyl terminal fragments (CTFs) in PC12 cells. Upregulation of RIN3 or the neuronal isoform of BIN1 increased phosphorylated Tau level. Therefore, upregulation of RIN3 expression promoted accumulation of APP CTFs and increased phosphorylated Tau. These effects by RIN3 was rescued by the expression of a dominant negative Rab5 (Rab5S34N) construct. Our study has thus pointed to that RIN3 acts through Rab5 to impact endosomal trafficking and signaling.ConclusionRIN3 is significantly upregulated and correlated with endosomal dysfunction in APP/PS1 mouse. Through interacting with BIN1 and CD2AP, increased RIN3 expression alters axonal trafficking and procession of APP. Together with our previous studies, our current work has thus provided important insights into the role of RIN3 in regulating endosomal signaling and trafficking.
- Published
- 2020
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