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6 results on '"PASQUATTI, Taynara N."'

1. Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae

Author

Bacanelli, Gisele, Olarte, Larissa C., Silva, Marcio Roberto, Rodrigues, Rudielle A., Carneiro, Paulo A. M., Kannene, John B., Pasquatti, Taynara N., Takatani, Haruo, Zumarraga, Martin Jose, Etges, Rodrigo N., Araujo, Flabio Ribeiro de, and Verbisck, Newton V.

Subjects
Espectrometría, Spectrometry, Bovina, Mycobacterium bovis Infections, Tuberculosis, Infeccion Mycobacterium tuberculosis, Desorption, Infeccion por Mycobacterium bovis, Ionization Treatment, Mycobacterium tuberculosis Infections, Desorción, Bovinae, Tratamiento Ionizante
Abstract

In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. Instituto de Biotecnología Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasil Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil Fil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil

Published
2019

4. Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae

Author

BACANELLI, Gisele, primary, OLARTE, Larissa C., additional, SILVA, Márcio R., additional, RODRIGUES, Rudielle A., additional, CARNEIRO, Paulo A.M., additional, KANEENE, John B., additional, PASQUATTI, Taynara N., additional, TAKATANI, Haruo, additional, ZUMÁRRAGA, Martin J., additional, ETGES, Rodrigo N., additional, ARAÚJO, Flábio R., additional, and VERBISCK, Newton V., additional

Published
2019
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5. Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil.

Author

Carneiro, Paulo A. M., Pasquatti, Taynara. N., Takatani, Haruo, Zumárraga, Martin J., Marfil, Maria. J., Barnard, Christian, Fitzgerald, Scott D., Abramovitch, Robert B., Araujo, Flábio R., and Kaneene, John B.

Subjects
*MYCOBACTERIAL diseases, *CATTLE, *BACTERIAL growth, *MYCOBACTERIUM bovis, *TUBERCULOSIS in cattle, *MYCOPLASMA bovis, *SLAUGHTERING
Abstract

The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil. [ABSTRACT FROM AUTHOR]

Published
2020
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