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Validation of an analytical method is a necessary step in controlling the quality of quantitative analysis. Method validation is an established process which provides documentary evidence that a system fulfils its pre-defined specification, or shows that an analytical method is acceptable for its intended purpose. The purpose of the present study was to validate an SPE-LC-MS/MS method for the determination of 17b-estradiol and estrone in surface water samples according to the requirements laid down in ISO 17025. The calibration curves, working ranges, recoveries, detection and quantification limits, trueness as well as repeatability were determined. The uncertainty budget was estimated following a top-down approach based on in-house validation data. For 17-estradiol, the expanded relative uncertainty was estimated as 16 and 15% at low and high concentration levels respectively, based on 15 replicate measurements on 5 days, and as 50 and 44% for a single measurement performed on a single day. For estrone, the expanded relative uncertainty was estimated as 13 and 12% at low and high concentration levels respectively, based on 15 replicate measurements on 5 days, and as 42 and 38% for a single measurement performed on a single day., JRC.D.2-Water and Marine Resources

Validation of an analytical method is a necessary step in controlling the quality of quantitative analysis. Method validation is an established process which provides documentary evidence that a system fulfills its pre-defined specification, or shows that an analytical method is acceptable for its intended purpose. The purpose of the present study was to validate an SPE-LC-MS/MS method for the determination of diclofenac in surface water samples according to the requirements laid down in ISO 17025. The calibration curves, working ranges, recoveries, detection and quantification limits, trueness as well as repeatability were determined. The uncertainty budget was estimated following a top-down approach based on in-house validation data. The expanded relative uncertainty was estimated as 13 and 8% at low and high concentration levels respectively, based on 15 replicate measurements on 5 days, and as 30 and 18% for a single measurement performed on a single day., JRC.H.1-Water Resources

This report describes work conducted by the European Commission’s Joint Research Centre in the context of its support to the implementation of the Water Framework Directive 2000/60/EC. The work aimed at the feasibility assessment of an experimental monitoring exercise in support to a so-called Watch List Mechanism in a collaborative design involving EU Member States laboratories and some 200 official monitoring station operated by the Member States. The report includes all details on sampling stations, performance of analytical methods as well as the results of the analyses of all samples with regard to the occurrence and levels of 20 compounds of concern. In total, 219 whole water samples originating from 25 EU Member States and 2 other European countries, were assessed for contents of acesulfame, glyphosate and its metabolite AMPA, 1H-Benzotriazole and tolyltriazoles, bisphenol A, triclosan and triclocarban, carbamazepine and its metabolite 10,11-dihydro-10,11-dihydroxycarbamazepine, sulfamethoxazole, perfluoropropionic acid, tris-2-chloropropyl phosphate, methyl tert-butyl ether, silver, boron and chloride (Cl-) in water. Furthermore, 23 sediment samples were analysed for decabromodiphenylethane and decabromodiphenyl ether. The underlying analytical methods are carefully documented with regards to their performance characteristics. Obtained results are assessed statistically and where possible compared to other findings. Although the analysed single samples are insufficient to make any statement on the performance of the treatment processes leading to the compost, the collective of data allows having a glance at the pan-European situation as regards the studies compounds. Background information from literature describing the situation before the survey is included, too. The report is divided into a core part and two annexes. For practical reasons, the report is split into two volumes: Volume 1 contains the report and the single analytical results; volume 2 contains the documentation of the sampling stations., JRC.H.1-Water Resources

This report describes work conducted by the European Commission’s Joint Research Centre (JRC) in the context of an Administrative Arrangement between DG Environment and the JRC. This work aimed at the generation, within a limited timeframe, of a large amount of analytical data, with high scientific and statistical value, for a number of compost and digestate types (afterwards referred to as COMDIG samples), to help provide a general overview and estimation of that possible variability within and between different COMDIG materials. The report includes the results of a targeted and independent screening of typical European situations of COMDIG materials with regard to the occurrence and levels of compounds of concern, many of which have never been assessed at a pan-European level. In total, 139 samples, mostly taken as grab samples and originating from 15 countries, were assessed for 22 minor and trace elements and 92 organic compounds including ingredients of personal care products and pharmaceuticals. The underlying analytical methods are carefully documented with regard to their performance characteristics. Where available, the so-called “horizontal” standards were followed. The results obtained are assessed statistically. Although the analysed single samples are insufficient to make any statement on the performance of the treatment processes leading to COMDIG samples, this collective of data provide a glimpse of the pan-European situation as regards the studied compounds., JRC.H.1-Water Resources

Validation of an analytical method is a necessary step in controlling the quality of quantitative analysis. Method validation is an established process which is the provision of documentary evidence that a system fulfills its pre-defined specification or the process of providing that an analytical method is acceptable for its intended purpose. The objectives of the present study were: • to validate a SPE-LC-MS/MS method for the determination of carbamazepine (CBZ), 10,11-dihydro-10,11-dihydroxy-carbamazepine (CBZ-DiOH), sulfamethoxazole (SMZ) and pentafluoropropionic acid (PFPrA) in surface water samples; • to validate a SPE-GC-MS method for the determination of tris (1-chloro-2-propyl) phosphate (TCPP) content in surface water samples. Method validations were performed according to the ISO 17025 requirement and the BT/TF151 WI CSS 99026 document. The calibration curves, working ranges, recoveries, detection and quantification limits, trueness as well as repeatability were determined. The budget uncertainty was also estimated following a top-down approach based on in-house validation data., JRC.H.1-Water Resources

This report describes the technical support provided to identify and prioritize among relevant compounds that have to be considered in European regulation dealing with Sewage Sludge. The work includes the results of a screening of typical European situations of sewage sludges with regard to the occurrence and levels of compounds of concern, many of which never assessed in a pan-European dimension. In total, 63 samples originating from 15 countries, were assessed for 22 minor and trace elements and 92 organic compounds including ingredients of personal care products and pharmaceuticals. Obtained results are assessed statistically and where possible compared to other findings. Background information from literature describing the situation before the survey is included. To assess the availability of selected persistent organic pollutants, findings of along-term case study are included, too., JRC.H.1-Water Resources