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The endoplasmic reticulum (ER) is a single-copy organelle that cannot be generated de novo, suggesting coordination between the mechanisms overseeing ER integrity and those controlling the cell cycle to maintain organelle inheritance. The Unfolded Protein Response (UPR) is a conserved signaling network that regulates ER homeostasis. Here, we show that pharmacological and genetic inhibition of the UPR sensors IRE1, ATF6, and PERK in unstressed cells delays the cell cycle, with PERK inhibition showing the most penetrant effect, which was associated with a slowdown of the G1-to-S/G2 transition. Treatment with the small molecule ISRIB to bypass the effects of PERK-dependent phosphorylation of the translation initiation factor eIF2α had no such effect, suggesting that cell cycle timing depends on PERKs kinase activity but is independent of eIF2α phosphorylation. Using complementary light and electron microscopy and flow cytometry-based analyses, we also demonstrate that the ER enlarges before mitosis. Together, our results suggest coordination between UPR signaling and the cell cycle to maintain ER physiology during cell division.

The closure of schools, social hubs, and extracurricular activities due to lockdown measures imposed to curb the spread of SARS-CoV-2, has increased the risk factors for students' mental health. This cross-sectional study, conducted from March 2020 to March 2021, aimed to estimate socio-emotional, cognitive, and affective disorders and substance use in a sample of first- and second-grade high school students in Northern Italy. This study compared data from 284 Italian students' self-perceptions along with the perceptions of their parents and teachers through a web-based survey. The differences in the perceptions of the three groups (students, parents, and teachers) were analyzed using an analysis of variance test, applying a Bonferroni correction. The X[superscript 2] test was used to assess the comparison between students, parents, and teachers in the substance use questions. The results showed statistically significant differences among the three groups. The most important outcomes were sociality, scholastic performance, extracurricular activity, emotional symptoms, affective disorders (depression and anxiety), and substance use. These findings could be interesting for the promotion of mental health and prevention of psychopathological risks in students.

ABSTRACT During its cell cycle, the bacterium Caulobacter crescentus switches from a motile, free-living state, to a sessile surface-attached cell. During this coordinated process, cells undergo irreversible morphological changes, such as shedding of their polar flagellum and synthesis of an adhesive holdfast at the same pole. In this work, we used genetic screens to identify genes involved in the regulation of the transition from the motile to the sessile lifestyle. We identified a predicted hybrid histidine kinase that inhibits biofilm formation and promotes the motile lifestyle: HmrA (holdfast and motility regulator A). Genetic screens and genomic localization led to the identification of additional genes that form a putative phosphorelay pathway with HmrA. We postulate that the Hmr pathway acts as a rheostat to control the proportion of cells harboring a flagellum or a holdfast in the population. Further genetic analysis suggests that the Hmr pathway impacts c-di-GMP synthesis through the diguanylate cyclase DgcB pathway. Our results also indicate that the Hmr pathway is involved in the regulation of motile to sessile lifestyle transition as a function of various environmental factors: biofilm formation is repressed when excess copper is present and derepressed under non-optimal temperatures. Finally, we provide evidence that the Hmr pathway regulates motility and adhesion without modulating the transcription of the holdfast synthesis regulator HfiA.IMPORTANCEComplex communities attached to a surface, or biofilms, represent the major lifestyle of bacteria in the environment. Such a sessile state enables the inhabitants to be more resistant to adverse environmental conditions. Thus, having a deeper understanding of the underlying mechanisms that regulate the transition between the motile and the sessile states could help design strategies to improve biofilms when they are beneficial or impede them when they are detrimental. For Caulobacter crescentus motile cells, the transition to the sessile lifestyle is irreversible, and this decision is regulated at several levels. In this work, we describe a putative phosphorelay that promotes the motile lifestyle and inhibits biofilm formation, providing new insights into the control of adhesin production that leads to the formation of biofilms.

IntroductionAsthma is a chronic inflammatory respiratory disease often associated with comorbidities. Among cardiovascular comorbidities, arterial hypertension seems to create an additional health burden in asthmatics. However, evidence on this relationship is lacking.ObjectiveOur study aims to evaluate the characteristics of hypertensive asthmatics, focusing on the role of inflammation as a possible link between these diseases.MethodsWe conducted a monocentric retrospective analysis consecutively including asthmatics who underwent induced sputum (IS) at our asthma referral center. Patients were divided in two groups according to presence or absence of history of hypertension. Clinical, functional, and inflammatory (airway and systemic) data were collected.ResultsData on two hundred and sixty asthmatic patients were analyzed. Seventy-nine (30.4%) of them had a diagnosis of hypertension requiring a specific pharmacological treatment. Asthmatics with hypertension were more frequently male (p = 0.047), older (p 0.05); distribution of comorbidities and lung function impairment (forced expiratory volume in the first second (FEV1) and forced vital capacity (FVC); all p

Study region: Four perialpine lakes in Switzerland, with different degrees of lake level management. Study focus: Alpine regions are particularly sensitive to climate change due to the pronounced effect on snow and glacial melt. In this context, large perialpine lakes play a crucial role in modulating climate change impacts on water resources, which brings together diverse interests. However, climate change studies on river systems rarely include lakes or lake level management. An open question is how to incorporate lake level management effects into hydrologic simulations to project climate change impacts. We combine the hydrologic model PREVAH with the hydrodynamic model MIKE11 to simulate lake level and outflow scenarios from 1981 to 2099, using the Swiss climate change scenarios CH2018. New hydrological insights for the region: The hydrological projections at the end of the century show pronounced seasonal changes in lake levels, characterised by an increase in winter and a decrease in summer when water demand is highest. Without climate mitigation measures, this summer decrease ranges from −0.04 m for a regulated lake to −0.4 m for an unregulated lake. In addition, the simulations indicate more frequent drought events. The projected changes intensify with time and missing climate mitigation measures. Future work could focus on interannual variability to explore regulatory strategies under changing conditions.

Abstract In the field of botany applied to archaeological and palaeoecological studies, the multi- and inter-disciplinary nature of this research produces a lack of data sharing and scattered articles in the specialty literature or in national and international journals. The vast production of archaeobotany and palynology data makes it necessary to develop a tool for the availability, accessibility, and dissemination of existing research. Many databases exist on palaeoecology, archaeobotany or pollen data. There are no collections focused on archaeological sites and human-induced environments and centred on Southern Europe and the Mediterranean. BRAIN - Botanical Records of Archaeobotany Italian Network is the first database listing sites from which all types of plant records are available in Italy and nearby Mediterranean regions. BRAIN represents the largest integrated collection of archaeo/palaeo-botanical data and a range of descriptive information that makes data recovery FAIR ready. This unique network hosts data on the availability of anthropogenic pollen, palynomorphs and plant macroremains in the same database, and experts of different research fields may contribute to it.

Abstract Background High blood eosinophils seem to predict exacerbations and response to inhaled corticosteroids (ICS) treatment in patients with chronic obstructive pulmonary disease (COPD). The aim of our study was to prospectively evaluate for 2 years, blood and sputum eosinophils in COPD patients treated with bronchodilators only at recruitment. Methods COPD patients in stable condition treated with bronchodilators only underwent monitoring of lung function, blood and sputum eosinophils, exacerbations and comorbidities every 6 months for 2 years. ICS was added during follow-up when symptoms worsened. Results 63 COPD patients were enrolled: 53 were followed for 1 year, 41 for 2 years, 10 dropped-out. After 2 years, ICS was added in 12/41 patients (29%) without any statistically significant difference at time points considered. Blood and sputum eosinophils did not change during follow-up. Only FEV1/FVC at T0 was predictive of ICS addition during the 2 year-follow-up (OR:0.91; 95% CI: 0.83–0.99, p = 0.03). ICS addition did not impact on delta (T24-T0) FEV1, blood and sputum eosinophils and exacerbations. After 2 years, patients who received ICS had higher blood eosinophils than those in bronchodilator therapy (p = 0.042). Patients with history of ischemic heart disease increased blood eosinophils after 2 years [p = 0.03 for both percentage and counts]. Conclusions Blood and sputum eosinophils remained stable during the 2 year follow-up and were not associated with worsened symptoms or exacerbations. Almost 30% of mild/moderate COPD patients in bronchodilator therapy at enrollment, received ICS for worsened symptoms in a 2 year-follow-up and only FEV1/FVC at T0 seems to predict this addition. History of ischemic heart disease seems to be associated with a progressive increase of blood eosinophils.

Understanding long-term committed climate change due to anthropogenic forcing is key to informing climate policies, yet these timescales are still underexplored. We present here a set of 1000-year-long abrupt stabilization simulations performed with EC-Earth3. Each simulation follows a sudden stabilization of the external forcing at the level specified by CMIP6 for historical (1990) or SSP5-8.5 scenario (2025, 2050, 2065, 2080, 2100) conditions, with a final temperature increase ranging between 1.4 and 9.6 K with respect to the pre-industrial baseline. Remarkably, the simulation stabilized at a greenhouse gas (GHG) level close to the present day (2025) exceeds the Paris Agreement goals of 1.5 and 2° warming above pre-industrial in the long term, and only the 1990 simulation leads to a stabilized climate below 1.5° warming. We first focus on the evolution of the climate response at multi-centennial timescales and its dependence on the level of forcing. We note a decrease in the magnitude of the climate feedback parameter at longer timescales. Conversely, simulations with higher forcing exhibit a larger feedback parameter (in magnitude). Subsequently, the evolution of surface warming patterns over multi-centennial timescales is studied. While the response is generally consistent across simulations, some variations, particularly in the South Pacific and at high latitudes, suggest a certain level of state or forcing dependence. The patterns of precipitation change also evolve during the stabilization runs: the drying trends found in the subtropical oceans and in Mediterranean-like hotspots in the SSP5-8.5 scenario tend to be reduced or even reversed. We finally focus on the rate of heat storage in the global ocean, which is the main driver of the climate response at multi-centennial timescales. We find that the rate of warming of the deep ocean is almost independent from the amplitude of the forcing so that most of the additional heat remains in the upper layers at high forcing. This might be due – at least partly – to a decreased ventilation of the deep ocean, caused by changes in the Meridional Overturning Circulation (MOC). These results highlight the importance of studying multi-centennial timescales of climate change to better understand the response of the deep ocean, which will play a crucial role in determining the final state of the climate system once GHG concentrations are stabilized.

Abstract Time series analyses of solute concentrations in streamwater and precipitation are powerful tools for unraveling the interplay of hydrological and biogeochemical processes at the catchment scale. While such datasets are available for many sites around the world, they often lack the necessary temporal resolution or are limited in the number of solutes they encompass. Here we present a multi-year dataset encompassing daily records of major ions and a range of trace metals in both streamwater and precipitation in three catchments in the northern Swiss Pre-Alps. These time series capture the temporal variability observed in solute concentrations in response to storm events, snow melt, and dry summer conditions. This dataset additionally includes stable water isotope data as an extension of a publicly available isotope dataset collected concurrently at the same locations, and together these data can provide insights into a range of ecohydrological processes and enable a suite of analyses into hydrologic and biogeochemical catchment functioning.

By uncovering novel aspects of second harmonic generation in aluminum, we show that there are unusual and remarkable consequences of resonant absorption, namely an unexpectedly critical role that bound electrons play for light–matter interactions across the optical spectrum, suggesting that a different basic approach is required to fully explain the physics of surfaces. We tackle an issue that is never under consideration given the generic hostile conditions to the propagation of light under resonant absorption. Unlike most noble metals, aluminum displays Lorentz-like behavior and interband transitions centered near 810 nm, thus splitting the plasmonic range in an atypical manner and setting its linear and nonlinear optical properties apart. Studies of aluminum nanostructures having complex topologies abound, as do reported inconsistencies in the linear spectral response of surface plasmons and harmonic generation. Our experimental observations of second harmonic generation from aluminum nanolayers show that bound electrons are responsible for a unique signature neither predicted nor observed previously: a hole in the second harmonic spectrum. A hydrodynamic-Maxwell theory explains these findings exceptionally well and becomes the basis for renewed studies of surface physics.

Additive Manufacturing provides unprecedented opportunities for designing and producing intricate, architected materials with exceptional properties while maintaining reduced weight. One of the most straightforward ways to create such structures is to mimic the lattice geometries of crystals. New approaches are now exploring how to influence the behaviour of these structures by mimicking not only the crystal arrangement but also peculiar mechanisms occurring at the crystallographic scale. In such a context, this study first presents a hexagonal close-packed (HCP) lattice derived from the homonymous crystal structures of Ti or Zr metals. Subsequently, the twinning phenomenon responsible for the plastic deformation of these metals is reproduced geometrically into this structure to improve its energy absorption capability. Compressive tests conducted on 3D-printed samples confirm such a hypothesis: the specific energy absorption of twinned structures is significantly higher than that of the equivalent HCP ones, with an increase of up to 24.3%. Introducing twinned regions stabilises the plastic deformation despite a limited reduction of the peak stress. Therefore, replicating the twinning metallurgical mechanism within an HCP-inspired metamaterial proves successful, further validating the approach of mimicking and transferring atomic phenomena at a different scale to tailor the properties of architected materials.

This special issue examines the complex and evolving relations between Japan and Southeast Asia. Historically rich in resources, Southeast Asia attracted imperial powers, including Japan, significantly transforming the region. Post-war ecosystems were influenced by Japanese occupation during WWII, shaping leaders and industrial development. Japanese investments and Official Development Assistance (ODA) since the 1960s have facilitated regional growth. Despite Japan's cautious diplomacy due to its US alliance, it diversified support in the 1990s, including infrastructure, legal, and political aid to transitional economies like Vietnam. Japan remains a significant investor, expanding aid to address climate change and maintaining trust in ASEAN countries. Recent geopolitical shifts, including China’s rise and US-China competition, have pushed Southeast Asia to strengthen ties with both powers, positioning Japan’s evolving role as crucial for regional security and development. The issue includes analyses of Japan’s strategic empowerment of Southeast Asia, capacity building, nostalgia in foreign policy, and smart technologies in urban planning, illustrating broader trends and reducing historical asymmetries.

INTRODUZIONE Il sonno è un processo fisiologico caratterizzato da una riduzione dello stato di coscienza e ha un impatto importante sulla qualità della vita (QoL). I disturbi del sonno sono comuni nelle persone affette da Long Covid, complessa condizione clinica caratterizzata dalla comparsa di sintomi a distanza di circa 3 mesi dall’infezione acuta da Sars-CoV-2. Lo scopo di questo studio è valutare le proprietà psicometriche della versione italiana della General Sleep Disturbance Scale (GSDS-IT) nelle persone affette da Long Covid. METODI Questo studio retrospettivo di valutazione delle qualità psicometriche della scala di valutazione General Sleep Disturbance Scale (GSDS) è stato condotto dal Team clinico-riabilitativo del Poliambulatorio San Felice della ASL Roma 2 dal 1 giugno al 10 luglio 2023 su un campione di 58 pazienti affetti da Long Covid. I criteri di inclusione erano l'ammissione ad un programma di riabilitazione respiratoria post-COVID-19; età minima 18 anni; capacità di comunicare; firma del consenso informato. La consistenza interna è stata determinata tramite l'alfa di Cronbach; per la validità di costrutto sono state somministrate la scala a 12 elementi ​​Short Form Health Survey (SF-12), le Attività di Daily Living (ADL) e le Attività Strumentali di Daily Living (IADL). RISULTATI I valori Alpha di Cronbach e la correlazione intra-classe (ICC) per tutte le sottoscale erano statisticamente significativi. È stata trovata una correlazione tra il sonno e la salute mentale, nonché per la scala totale e le ADL. CONCLUSIONI La GSDS è uno strumento valido e affidabile per la valutazione dei disturbi del sonno nei pazienti con Long Covid. Il suo utilizzo in questa tipologia di pazienti è suggerito per rilevare la presenza di disturbi del sonno, in modo che sia i medici che i ricercatori possano prendere le decisioni più appropriate per la cura globale dei pazienti e per migliorare la loro QoL.