23 results on '"P. Romo-Rodríguez"'
Search Results
2. Implementation of a roadmap for the comprehensive diagnosis, follow-up, and research of childhood leukemias in vulnerable regions of Mexico: results from the PRONAII Strategy
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Juan Carlos Núñez-Enríquez, Rubí Romo-Rodríguez, Pedro Gaspar-Mendoza, Gabriela Zamora-Herrera, Lizeth Torres-Pineda, Jiovanni Amador-Cardoso, Jebea A. López-Blanco, Laura Alfaro-Hernández, Lucero López-García, Arely Rosas-Cruz, Dulce Rosario Alberto-Aguilar, César Omar Trejo-Pichardo, Dalia Ramírez-Ramírez, Astin Cruz-Maza, Janet Flores-Lujano, Nuria Luna-Silva, Angélica Martínez-Martell, Karina Martínez-Jose, Anabel Ramírez-Ramírez, Juan Carlos Solis-Poblano, Patricia Zagoya-Martínez, Vanessa Terán-Cerqueda, Andrea Huerta-Moreno, Álvaro Montiel-Jarquín, Miguel Garrido-Hernández, Raquel Hernández-Ramos, Daniela Olvera-Caraza, Cynthia Shanat Cruz-Medina, Enoch Alvarez-Rodríguez, Lénica Anahí Chávez-Aguilar, Wilfrido Herrera-Olivares, Brianda García-Hidalgo, Lena Sarahí Cano-Cuapio, Claudia Guevara-Espejel, Gerardo Juárez-Avendaño, Juan Carlos Balandrán, Ma. del Rocío Baños-Lara, Mariana Cárdenas-González, Elena R. Álvarez-Buylla, Sonia Mayra Pérez-Tapia, Diana Casique-Aguirre, and Rosana Pelayo
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childhood leukemias ,Mexico ,measurable residual disease (MRD) ,diagnosis ,vulnerable regions ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The main objective of the National Project for Research and Incidence of Childhood Leukemias is to reduce early mortality rates for these neoplasms in the vulnerable regions of Mexico. This project was conducted in the states of Oaxaca, Puebla, and Tlaxcala. A key strategy of the project is the implementation of an effective roadmap to ensure that leukemia patients are the target of maximum benefit of interdisciplinary collaboration between researchers, clinicians, surveyors, and laboratories. This strategy guarantees the comprehensive management of diagnosis and follow-up samples of pediatric patients with leukemia, centralizing, managing, and analyzing the information collected. Additionally, it allows for a precise diagnosis and monitoring of the disease through immunophenotype and measurable residual disease (MRD) studies, enhancing research and supporting informed clinical decisions for the first time in these regions through a population-based study. This initiative has significantly improved the diagnostic capacity of leukemia in girls, boys, and adolescents in the regions of Oaxaca, Puebla, and Tlaxcala, providing comprehensive, high-quality care with full coverage in the region. Likewise, it has strengthened collaboration between health institutions, researchers, and professionals in the sector, which contributes to reducing the impact of the disease on the community.
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- 2024
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3. Subclassification of B-acute lymphoblastic leukemia according to age, immunophenotype and microenvironment, predicts MRD risk in Mexican children from vulnerable regions
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Rubí Romo-Rodríguez, Gabriela Zamora-Herrera, Jebea A. López-Blanco, Lucero López-García, Arely Rosas-Cruz, Laura Alfaro-Hernández, César Omar Trejo-Pichardo, Dulce Rosario Alberto-Aguilar, Diana Casique-Aguirre, Armando Vilchis-Ordoñez, Juan Carlos Solis-Poblano, Lilia Adela García-Stivalet, Vanessa Terán-Cerqueda, Nuria Citlalli Luna-Silva, Miguel Ángel Garrido-Hernández, Lena Sarahí Cano-Cuapio, Karen Ayala-Contreras, Fabiola Domínguez, María de los Ángeles del Campo-Martínez, Gerardo Juárez-Avendaño, Juan Carlos Balandrán, Sonia Mayra Pérez-Tapia, Carlos Fernández-Giménez, Pedro A. Zárate-Rodríguez, Enrique López-Aguilar, Aurora Treviño-García, Célida Duque-Molina, Laura C. Bonifaz, Juan Carlos Núñez-Enríquez, Mariana Cárdenas-González, Elena R. Álvarez-Buylla, Dalia Ramírez-Ramírez, and Rosana Pelayo
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tumor microenvironment ,risk stratification ,ProB-ALL ,measurable residual disease (MRD) ,Mexican children ,acute leukemia ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
IntroductionThe decisive key to disease-free survival in B-cell precursor acute lymphoblastic leukemia in children, is the combination of diagnostic timeliness and treatment efficacy, guided by accurate patient risk stratification. Implementation of standardized and high-precision diagnostic/prognostic systems is particularly important in the most marginalized geographic areas in Mexico, where high numbers of the pediatric population resides and the highest relapse and early death rates due to acute leukemias are recorded even in those cases diagnosed as standard risk.MethodsBy using a multidimensional and integrated analysis of the immunophenotype of leukemic cells, the immunological context and the tumor microenvironment, this study aim to capture the snapshot of acute leukemia at disease debut of a cohort of Mexican children from vulnerable regions in Puebla, Oaxaca and Tlaxcala and its potential use in risk stratification.Results and discussionOur findings highlight the existence of a distinct profile of ProB-ALL in children older than 10 years, which is associated with a six-fold increase in the risk of developing measurable residual disease (MRD). Along with the absence of CD34+ seminal cells for normal hematopoiesis, this ProB-ALL subtype exhibited several characteristics related to poor prognosis, including the high expression level of myeloid lineage markers such as MPO and CD33, as well as upregulation of CD19, CD34, CD24, CD20 and nuTdT. In contrast, it showed a trend towards decreased expression of CD9, CD81, CD123, CD13, CD15 and CD21. Of note, the mesenchymal stromal cell compartment constituting their leukemic niche in the bone marrow, displayed characteristics of potential suppressive microenvironment, such as the expression of Gal9 and IDO1, and the absence of the chemokine CXCL11. Accordingly, adaptive immunity components were poorly represented. Taken together, our results suggest, for the first time, that a biologically distinct subtype of ProB-ALL emerges in vulnerable adolescents, with a high risk of developing MRD. Rigorous research on potential enhancing factors, environmental or lifestyle, is crucial for its detection and prevention. The use of the reported profile for early risk stratification is suggested.
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- 2024
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4. Tumor de células gigantes recidivante de radio distal: tratamiento con resección y reconstrucción con transposición ulnar
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Marie C. Pellat-Fons, Ranulfo Romo-Rodríguez, Fernanda Rodríguez-Reyes, and Raúl Álvarez-San Martín
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Tumor de células gigantes. Tumor recidivante. Transposición cubital. ,Medicine - Abstract
Introducción. El radio distal es la tercera localización más común para un tumor de células gigantes. El tratamiento con legrado y cementación de una lesión Campanacci grado II puede dar cabida a una recidiva de la tumoración. Objetivo. Describir una novedosa técnica que permite realizar una reconstrucción del radio distal tras una resección en bloque. Material y métodos. Se presenta un caso clínico poco común de un tumor de células gigantes recidivante de radio distal, así como el resultado funcional y clínico tras su resección y posterior reconstrucción con una transposición del decúbito ipsilateral como un injerto óseo vascularizado, asociado a una artrodesis de la muñeca. Posterior a la intervención el paciente se presenta libre de enfermedad, con arcos de movimiento completos y se encuentra asintomático y conforme con la cosmesis de su muñeca y su antebrazo. Esta es una técnica segura que presenta excelentes resultados funcionales y clínicos, no ocasiona morbilidad en otras extremidades y no requiere del empleo de una técnica microquirúrgica o de bancos óseos.
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- 2023
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5. Chloroquine Induces ROS-mediated Macrophage Migration Inhibitory Factor Secretion and Epithelial to Mesenchymal Transition in ER-positive Breast Cancer Cell Lines
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Rojas-Sanchez, Guadalupe, García-Miranda, Alin, Montes-Alvarado, José Benito, Cotzomi-Ortega, Israel, Sarmiento-Salinas, Fabiola Lilí, Jimenez-Ignacio, Eduardo Eleazar, Ramírez-Ramírez, Dalia, Romo-Rodríguez, Rubí Esmeralda, Reyes-Leyva, Julio, Vallejo-Ruiz, Verónica, Pazos-Salazar, Nidia Gary, and Maycotte, Paola
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- 2021
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6. Total Elbow Arthroplasty and Antegrade Posterior Interosseous Flap for Infected Posttraumatic Arthritis with an Active Fistula. A Rationale for Comprehensive Treatment. Case Report
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Efraín Farías-Cisneros MD, PhD, FACS, Jorge Luis Martínez-Peniche MD, Luis Carlos Olguín-Delgado MD, Francisco Guillermo Castillo-Vázquez MD, Ranulfo Romo-Rodríguez MD, FACS, and Armando Torres-Gómez MD, MSc, FACS
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Orthopedic surgery ,RD701-811 - Abstract
The indication for total elbow arthroplasty (TEA) for primary and posttraumatic elbow arthritis has increased, however, its indication after infection remains elusive. Wound closure about the elbow increases the challenge of treating a previously infected elbow, often necessitating soft tissue coverage with local or regional flaps. We present a 75-year-old male patient with an elbow infection following a failed complex intraarticular fracture open reduction and internal fixation of the distal humerus. Initially, he presented with severe functional impairment and pain, also with an active fistula with serous exudate, whose culture was positive for Cutinebacterium acnes . Septic hardware loosening, and septic nonunion with intraarticular involvement of the left elbow was diagnosed. The patient underwent hardware removal, fistulectomy, serial irrigation and debridement and a pedicled antegrade posterior interosseous artery (PIA) flap on staged surgical treatment. Finally, after ruling out infection persistence, a TEA was performed. We aim to report the outcome of a patient treated with a TEA in the context of a previously infected elbow with soft tissue coverage with an antegrade PIA flap. Comprehensive treatment must be done in an appropriate manner, to obtain an expedited and desirable outcome.
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- 2022
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7. Alcohol dehydrogenase 1 participates in the Crabtree effect and connects fermentative and oxidative metabolism in the Zygomycete Mucor circinelloides
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Rangel-Porras, Rosa Angélica, Díaz-Pérez, Sharel P., Mendoza-Hernández, Juan Manuel, Romo-Rodríguez, Pamela, Alejandre-Castañeda, Viridiana, Valle-Maldonado, Marco I., Torres-Guzmán, Juan Carlos, González-Hernández, Gloria Angélica, Campos-Garcia, Jesús, Arnau, José, Meza-Carmen, Víctor, and Gutiérrez-Corona, J. Félix
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- 2019
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8. Aerobic processes for bioleaching manganese and silver using microorganisms indigenous to mine tailings
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Huerta-Rosas, B., Cano-Rodríguez, I., Gamiño-Arroyo, Z., Gómez-Castro, F. I., Carrillo-Pedroza, F. R., Romo-Rodríguez, P., and Gutiérrez-Corona, J. F.
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- 2020
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9. Microbial interactions with chromium: basic biological processes and applications in environmental biotechnology
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Gutiérrez-Corona, J. F., Romo-Rodríguez, P., Santos-Escobar, F., Espino-Saldaña, A. E., and Hernández-Escoto, H.
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- 2016
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10. Atomistic mechanism of leptin and leptin-receptor association
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López-Hidalgo, Marisol, Caro-Gómez, Luis A., Romo-Rodríguez, Rubí, Herrera-Zuñiga, Leonardo D., Anaya-Reyes, Maricruz, Rosas-Trigueros, Jorge L., and Benítez-Cardoza, Claudia G.
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AbstractThe leptin-leptin receptor complex is at the very core of energy homeostasis and immune system regulation, among many other functions. In this work, we built homology models of leptin and the leptin binding domain (LBD) of the receptor from humans and mice. Docking analyses were used to obtain the coordinates of the native leptin-LBD complexes and a mixed heterodimer formed by human leptin and mouse LBD. Molecular dynamics (MD) simulations were performed using all models (monomers and heterodimers) as initial coordinates and the GROMACS program. The overall structural and dynamical behaviors are similar for the three complexes. Upon MD simulations, several new interactions appear. In particular, hydrophobic interactions, with more than 90% persistence, seem to be the most relevant for the stability of the dimers, as well as the pair formed by Asp85Lepand Arg468LBD. This in silicoanalysis provides structural and dynamical information, at the atomistic level, about the mechanism of leptin-LBD complex formation and leptin receptor activation. This knowledge might be used in the rational drug design of therapeutics to modulate leptin signaling.Communicated by Ramaswamy H. Sarma
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- 2023
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11. Surgical Solution for Total Carpectomy due to Destructive Wrist Pan-Osteomyelitis Using a Free Femoral Condyle Osteocutaneous Flap for Wrist Arthrodesis
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Castillo-Vázquez, Francisco Guillermo, Palafox-Carral, Ignacio, Romo-Rodríguez, Ranulfo, Limón-Muñoz, Marisol, and Farías-Cisneros, Efraín
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Osteomyelitis of the hand is rare, even more so in the carpal bones. Patients with rheumatoid arthritis (RA) have a higher infection rate overall, and up to a 14-fold increase in the incidence of septic arthritis of the hand. The destruction of immunologic barriers, such as cartilage and joint capsules, as well as the use of immunosuppressive medications will have an impact on the higher incidence of articular infections and osteomyelitis in these patients. Infection in these cases is often overlooked because of the similarity of presentation to an acute event of RA. When osteomyelitis is present, rapid and aggressive treatment should be given. Surgical debridement, lavage, and excision of necrotic bone is the best choice, followed by cemented antibiotic impregnated spacer to resolve the acute scenario. Vascularized bone grafts (VBG) can then be used for a definitive solution, as these have great biologic properties that increase the possibility of a good outcome. We hereby present a report of a wrist arthrodesis, using a free medial femoral condyle VBG for the treatment of destructive osteomyelitis of the carpal bones in a female patient with RA.
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- 2023
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12. Aerobic processes for bioleaching manganese and silver using microorganisms indigenous to mine tailings
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F. R. Carrillo-Pedroza, B. Huerta-Rosas, I. Cano-Rodríguez, Zeferino Gamiño-Arroyo, Fernando Israel Gómez-Castro, P. Romo-Rodríguez, and J. F. Gutiérrez-Corona
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0106 biological sciences ,Silver ,Physiology ,Microorganism ,chemistry.chemical_element ,Industrial Waste ,Manganese ,01 natural sciences ,Applied Microbiology and Biotechnology ,Sphingomonas ,Mining ,Metal ,03 medical and health sciences ,010608 biotechnology ,Bioleaching ,Metals, Heavy ,Mexico ,0303 health sciences ,biology ,Bacteria ,030306 microbiology ,Chemistry ,Fungi ,General Medicine ,Drug Tolerance ,biology.organism_classification ,Penicillium chrysogenum ,Tailings ,Environmental chemistry ,visual_art ,visual_art.visual_art_medium ,Leaching (metallurgy) ,Biotechnology - Abstract
Mining wastes containing appreciable concentrations of silver are considered alternative sources for metal extraction, although these wastes are often refractory due to the presence of manganese oxides. The high cost and/or environmental impact of the hydrometallurgical and pyrometallurgical extraction processes make it necessary to search for biotechnological processes for the solubilization of manganese compounds. This paper describes the characterization of chemoorganotrophic microorganisms indigenous to the tailings of a silver mine located in Coahuila, Mexico, regarding their capability to remove manganese and silver present in these residues by lixiviation. The Bacterial and fungal strains isolated were identified by sequencing the rDNA 16S and ITS-1-ITS-2 genomic regions, respectively; the bacterial strains correspond to isolates of Roseospira sp. and Sphingomonas sp., whereas the fungal strains include isolates of Cladosporium sp. A, Cladosporium sp. B and Penicillium chrysogenum. These fungal strains show an effective capacity to lixiviate manganese and silver from solid mine residue when incubated in 9 k medium; it was found that under these conditions, leaching of metals occurs due to a mixed biotic-abiotic process, which yields manganese and silver leaching efficiencies in the ranges of 58–74% and 40–67%, respectively. The fungal strains grown in the LMM medium and the bacterial strains incubated in the PDB medium caused leaching of manganese with a lower efficiency in the range of 0.17–0.24% and 1.42–1.73%, respectively; under these conditions, silver leaching by fungal and bacterial strains appeared to be reduced (
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- 2020
13. Emergency Hematopoiesis in the Pathobiology of COVID-19: The Dark Side of an Early Innate Protective Mechanism
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Balandrán, Juan Carlos, Zamora-Herrera, Gabriela, Romo-Rodríguez, Rubí, and Pelayo, Rosana
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The recognition of pathogens to which we are constantly exposed induces the immediate replenishment of innate immune cells from the most primitive stages of their development through emergency hematopoiesis, a central mechanism contributing to early infection control. However, as with other protective mechanisms, its functional success is at risk when the excess of inducing signals accelerates immunological catastrophes. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection exhibits a clinical spectrum that ranges from completely asymptomatic states to fatal outcomes, with the amplification of inflammatory components being the critical point that determine the progress, complication, and severity of the disease. This review focuses on the most relevant findings that entail emergency hematopoiesis to SARS-CoV-2 infection response and revolutionize our understanding of the mechanisms governing the clinical prognosis of COVID-19. Of special interest are the metabolic or hyperinflammatory conditions in aging that exacerbate the phenomenon and favor the uncontrolled emergency myelopoiesis leading to the evolution of severe disease.
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- 2022
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14. Fungal Processes of Interaction with Chromium
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P. Romo-Rodríguez and J. F. Gutiérrez-Corona
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Chromium ,Chemistry ,Environmental chemistry ,chemistry.chemical_element - Published
- 2019
15. Alcohol dehydrogenase 1 participates in the Crabtree effect and connects fermentative and oxidative metabolism in the Zygomycete Mucor circinelloides
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Juan Manuel Mendoza-Hernández, Jesús Campos-García, P. Romo-Rodríguez, J. Félix Gutiérrez-Corona, Sharel P. Díaz-Pérez, Juan Carlos Torres-Guzmán, Rosa Angélica Rangel-Porras, Gloria Angélica González-Hernández, Víctor Meza-Carmen, José Arnau, Marco I. Valle-Maldonado, and Viridiana Alejandre-Castañeda
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Mutant ,Applied Microbiology and Biotechnology ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Oxidative enzyme ,030304 developmental biology ,Alcohol dehydrogenase ,0303 health sciences ,Ethanol ,biology ,030306 microbiology ,Chemistry ,Alcohol Dehydrogenase ,General Medicine ,biology.organism_classification ,Complementation ,Glucose ,Biochemistry ,Mucor ,Mucor circinelloides ,Fermentation ,biology.protein ,Crabtree effect ,NAD+ kinase ,Energy Metabolism ,Oxidation-Reduction - Abstract
Mucor circinelloides is a dimorphic Zygomycete fungus that produces ethanol under aerobic conditions in the presence of glucose, which indicates that it is a Crabtree-positive fungus. To determine the physiological role of the alcohol dehydrogenase (ADH) activity elicited under these conditions, we obtained and characterized an allyl alcohol-resistant mutant that was defective in ADH activity, and examined the effect of adh mutation on physiological parameters related to carbon and energy metabolism. Compared to the Adh+ strain R7B, the ADH-defective (Adh-) strain M5 was unable to grow under anaerobic conditions, exhibited a considerable reduction in ethanol production in aerobic cultures when incubated with glucose, had markedly reduced growth capacity in the presence of oxygen when ethanol was the sole carbon source, and exhibited very low levels of NAD+-dependent alcohol de-hydrogenase activity in the cytosolic fraction. Further characterization of the M5 strain showed that it contains a 10-bp deletion that interrupts the coding region of the adhl gene. Complementation with the wild-type allele adh1+ by transformation of M5 remedied all the defects caused by the adh1 mutation. These findings indicate that in M. circinelloides, the product of the adh1 gene mediates the Crabtree effect, and can act as either a fermentative or an oxidative enzyme, depending on the nutritional conditions, thereby participating in the association between fermentative and oxidative metabolism. It was found that the spores of M. circinelloides possess low mRNA levels of the ethanol assimilation genes (adl2 and acs2), which could explain their inability to grow in the alcohol.
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- 2018
16. CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid
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Karen C. Hernández-Ramírez, Martha I. Ramírez-Díaz, P. Romo-Rodríguez, Rocio V. Perez-Gallardo, Víctor Meza-Carmen, Juan Pablo García-Merinos, Víctor M. Chávez-Jacobo, Jesus Silva-Sanchez, J. Félix Gutiérrez-Corona, and Jesús Campos-García
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0301 basic medicine ,Virulence Factors ,030106 microbiology ,Quinolones ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,Plasmid ,Shuttle vector ,Bacterial Proteins ,Mechanisms of Resistance ,Ciprofloxacin ,medicine ,Pharmacology (medical) ,Pseudomonas Infections ,Phosphorylation ,Escherichia coli ,Norfloxacin ,Pharmacology ,biology ,Chemistry ,Pseudomonas aeruginosa ,Mycobacterium smegmatis ,Aminoglycoside ,Gene Expression Regulation, Bacterial ,Plasmid-mediated resistance ,biology.organism_classification ,030104 developmental biology ,Infectious Diseases ,medicine.drug ,Plasmids - Abstract
The pUM505 plasmid, isolated from a clinical Pseudomonas aeruginosa isolate, confers resistance to ciprofloxacin (CIP) when transferred into the standard P. aeruginosa strain PAO1. CIP is an antibiotic of the quinolone family that is used to treat P. aeruginosa infections. In silico analysis, performed to identify CIP resistance genes, revealed that the 65-amino-acid product encoded by the orf131 gene in pUM505 displays 40% amino acid identity to the Mycobacterium smegmatis aminoglycoside phosphotransferase (an enzyme that phosphorylates and inactivates aminoglycoside antibiotics). We cloned orf131 (renamed crpP , for c iprofloxacin r esistance p rotein, p lasmid encoded) into the pUCP20 shuttle vector. The resulting recombinant plasmid, pUC- crpP , conferred resistance to CIP on Escherichia coli strain J53-3, suggesting that this gene encodes a protein involved in CIP resistance. Using coupled enzymatic analysis, we determined that the activity of CrpP on CIP is ATP dependent, while little activity against norfloxacin was detected, suggesting that CIP may undergo phosphorylation. Using a recombinant His-tagged CrpP protein and liquid chromatography–tandem mass spectrometry, we also showed that CIP was phosphorylated prior to its degradation. Thus, our findings demonstrate that CrpP, encoded on the pUM505 plasmid, represents a new mechanism of CIP resistance in P. aeruginosa , which involves phosphorylation of the antibiotic.
- Published
- 2018
17. Surgical Solution for Total Carpectomy due to Destructive Wrist Pan-Osteomyelitis Using a Free Femoral Condyle Osteocutaneous Flap for Wrist Arthrodesis
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Castillo-Vázquez, Francisco Guillermo, Palafox-Carral, Ignacio, Romo-Rodríguez, Ranulfo, Limón-Muñoz, Marisol, and Farías-Cisneros, Efraín
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- 2022
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18. Cr(VI) reduction by gluconolactone and hydrogen peroxide, the reaction products of fungal glucose oxidase: Cooperative interaction with organic acids in the biotransformation of Cr(VI)
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Francisco Javier Acevedo-Aguilar, Adolfo López-Torres, J. Félix Gutiérrez-Corona, Katarzyna Wrobel, Kazimierz Wrobel, and P. Romo-Rodríguez
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Chromium ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Radical ,Gluconates ,Gluconolactone ,Glucose Oxidase ,Lactones ,chemistry.chemical_compound ,Biotransformation ,Extracellular ,Soil Pollutants ,Environmental Chemistry ,Organic chemistry ,Glucose oxidase ,Organic Chemicals ,Hydrogen peroxide ,chemistry.chemical_classification ,biology ,Strain (chemistry) ,Chemistry ,Public Health, Environmental and Occupational Health ,Hydrogen Peroxide ,General Medicine ,General Chemistry ,Pollution ,Enzyme ,biology.protein ,Acids ,Oxidation-Reduction ,Nuclear chemistry - Abstract
The Cr(VI) reducing capability of growing cells of the environmental A. tubingensis Ed8 strain is remarkably efficient compared to reference strains A. niger FGSC322 and A. tubingensis NRRL593. Extracellular glucose oxidase (GOX) activity levels were clearly higher in colonies developed in solid medium and in concentrated extracts of the spent medium of liquid cultures of the Ed8 strain in comparison with the reference strains. In addition, concentrated extracts of the spent medium of A. tubingensis Ed8, but not those of the reference strains, exhibited the ability to reduce Cr(VI). In line with this observation, it was found that A. niger purified GOX is capable of mediating the conversion of Cr(VI) to Cr(III) in a reaction dependent on the presence of glucose that is stimulated by organic acids. Furthermore, it was found that a decrease in Cr(VI) may occur in the absence of the GOX enzyme, as long as the reaction products gluconolactone and hydrogen peroxide are present; this conversion of Cr(VI) is stimulated by organic acids in a reaction that generates hydroxyl radicals, which may involve the formation of an intermediate peroxichromate(V) complex. These findings indicated that fungal glucose oxidase acts an indirect chromate reductase through the formation of Cr(VI) reducing molecules, which interact cooperatively with other fungal metabolites in the biotransformation of Cr(VI).
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- 2015
19. Microbial interactions with chromium: basic biological processes and applications in environmental biotechnology
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P. Romo-Rodríguez, J. F. Gutiérrez-Corona, Fernando Santos-Escobar, Ángeles E. Espino-Saldaña, and Héctor Hernández-Escoto
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0301 basic medicine ,Chromium ,Proteomics ,Physiology ,Microorganism ,chemistry.chemical_element ,010501 environmental sciences ,01 natural sciences ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,Bioremediation ,Metabolomics ,0105 earth and related environmental sciences ,Pollutant ,Chromate conversion coating ,Bacteria ,Chemistry ,Gene Expression Profiling ,Biosorption ,Electron Spin Resonance Spectroscopy ,Fungi ,General Medicine ,030104 developmental biology ,Environmental biotechnology ,Biodegradation, Environmental ,X-Ray Absorption Spectroscopy ,Environmental chemistry ,Bioaccumulation ,Environmental Pollutants ,Biotechnology - Abstract
Chromium (Cr) is a highly toxic metal for microorganisms as well as plants and animal cells. Due to its widespread industrial use, Cr has become a serious pollutant in diverse environmental settings. The hexavalent form of the metal, Cr(VI), is considered a more toxic species than the relatively innocuous and less mobile Cr(III) form. The study of the interactions between microorganisms and Cr has been helpful to unravel the mechanisms allowing organisms to survive in the presence of high concentrations of Cr(VI) and to detoxify and remove the oxyanion. Various mechanisms of interactions with Cr have been identified in diverse species of bacteria and fungi, including biosorption, bioaccumulation, reduction of Cr(VI) to Cr(III), and chromate efflux. Some of these systems have been proposed as potential biotechnological tools for the bioremediation of Cr pollution using bioreactors or by in situ treatments. In this review, the interactions of microorganisms with Cr are summarised, emphasising the importance of new research avenues using advanced methodologies, including proteomic, transcriptomic, and metabolomic analyses, as well as the use of techniques based on X-ray absorption spectroscopy and electron paramagnetic resonance spectroscopy.
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- 2016
20. Cistoadenoma seroso gigante de ovario y embarazo a término. Reporte de un caso
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Villafán-Cedeño L, L., Vega-Malagón, G., Rivera, M., Romo-Rodríguez, M.R., and Becerril-Santos, A.
- Abstract
Se presenta el caso clínico de una mujer de 25 años de edad con embarazo de pretérmino y un adenoma seroso gigante de ovario resuelto quirúrgicamente, en el Hospital Regional No. 1 del IMSS de Querétaro.
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- 2015
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21. CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosapUM505 Plasmid
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Chávez-Jacobo, Víctor M., Hernández-Ramírez, Karen C., Romo-Rodríguez, Pamela, Pérez-Gallardo, Rocío Viridiana, Campos-García, Jesús, Gutiérrez-Corona, J. Félix, García-Merinos, Juan Pablo, Meza-Carmen, Víctor, Silva-Sánchez, Jesús, and Ramírez-Díaz, Martha I.
- Abstract
ABSTRACTThe pUM505 plasmid, isolated from a clinical Pseudomonas aeruginosaisolate, confers resistance to ciprofloxacin (CIP) when transferred into the standard P. aeruginosastrain PAO1. CIP is an antibiotic of the quinolone family that is used to treat P. aeruginosainfections. In silicoanalysis, performed to identify CIP resistance genes, revealed that the 65-amino-acid product encoded by the orf131gene in pUM505 displays 40% amino acid identity to the Mycobacterium smegmatisaminoglycoside phosphotransferase (an enzyme that phosphorylates and inactivates aminoglycoside antibiotics). We cloned orf131(renamed crpP, for ciprofloxacin resistance protein, plasmid encoded) into the pUCP20 shuttle vector. The resulting recombinant plasmid, pUC-crpP, conferred resistance to CIP on Escherichia colistrain J53-3, suggesting that this gene encodes a protein involved in CIP resistance. Using coupled enzymatic analysis, we determined that the activity of CrpP on CIP is ATP dependent, while little activity against norfloxacin was detected, suggesting that CIP may undergo phosphorylation. Using a recombinant His-tagged CrpP protein and liquid chromatography–tandem mass spectrometry, we also showed that CIP was phosphorylated prior to its degradation. Thus, our findings demonstrate that CrpP, encoded on the pUM505 plasmid, represents a new mechanism of CIP resistance in P. aeruginosa, which involves phosphorylation of the antibiotic.
- Published
- 2018
- Full Text
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22. Novel 2-aryl-4-aryloxyquinoline-based fungistatics for Mucor circinelloides. Biological evaluation of activity, QSAR and docking study.
- Author
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Nahide PD, Alba-Betancourt C, Chávez-Rivera R, Romo-Rodríguez P, Solís-Hernández M, Segura-Quezada LA, Torres-Carbajal KR, Gámez-Montaño R, Deveze-Álvarez MA, Ramírez-Morales MA, Alonso-Castro AJ, Zapata-Morales JR, Ruiz-Padilla AJ, Mendoza-Macías CL, Meza-Carmen V, Cortés-García CJ, Corrales-Escobosa AR, Núñez-Anita RE, Ortíz-Alvarado R, Chacón-García L, and Solorio-Alvarado CR
- Subjects
- Fluconazole, Humans, Mucor, Quantitative Structure-Activity Relationship, Mucormycosis drug therapy, Mucormycosis microbiology, Quinolines pharmacology, Quinolines therapeutic use
- Abstract
Zygomycetes are ubiquitous saprophytes in natural environments which transform organic matter. Some zygomycetes of gender Mucor have attracted interest in health sector. Due to its ability as opportunistic microorganisms infecting immuno-compromised people and to the few available pharmacological treatments, the mucormycosis is receiving worldwide attention. Concerning to the pharmacological treatments, some triazole-based compounds such as fluconazole are extensively used. Nevertheless, we focused in the quinolines since they are broadly used models for the design and development of new synthetic antifungal agents. In this study, the fungistatic activity on M. circinelloides of various 2-aryl-4-aryloxyquinoline-based compounds was discovered, and in some cases, it resulted better than reference compound fluconazole. These quinoline derivatives were synthesized via the C
sp 2 -O bond formation using diaryliodonium(III) salts chemistry. A QSAR study was carried out to quantitatively correlate the chemical structure of the tested compounds with their biological activity. Also, a docking study to identify a plausible action target of our more active quinolines was carried out. The results highlighted an increased activity with the fluorine- and nitro-containing derivatives. In light of the few mucormycosis pharmacological treatments, herein we present some non-described molecules with excellent in vitro activities and potential use in the mucormycosis treatment., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2022
- Full Text
- View/download PDF
23. Cr(VI) reduction by gluconolactone and hydrogen peroxide, the reaction products of fungal glucose oxidase: Cooperative interaction with organic acids in the biotransformation of Cr(VI).
- Author
-
Romo-Rodríguez P, Acevedo-Aguilar FJ, Lopez-Torres A, Wrobel K, Wrobel K, and Gutiérrez-Corona JF
- Subjects
- Acids chemistry, Organic Chemicals chemistry, Oxidation-Reduction, Chromium chemistry, Gluconates chemistry, Glucose Oxidase chemistry, Hydrogen Peroxide chemistry, Lactones chemistry, Soil Pollutants chemistry
- Abstract
The Cr(VI) reducing capability of growing cells of the environmental A. tubingensis Ed8 strain is remarkably efficient compared to reference strains A. niger FGSC322 and A. tubingensis NRRL593. Extracellular glucose oxidase (GOX) activity levels were clearly higher in colonies developed in solid medium and in concentrated extracts of the spent medium of liquid cultures of the Ed8 strain in comparison with the reference strains. In addition, concentrated extracts of the spent medium of A. tubingensis Ed8, but not those of the reference strains, exhibited the ability to reduce Cr(VI). In line with this observation, it was found that A. niger purified GOX is capable of mediating the conversion of Cr(VI) to Cr(III) in a reaction dependent on the presence of glucose that is stimulated by organic acids. Furthermore, it was found that a decrease in Cr(VI) may occur in the absence of the GOX enzyme, as long as the reaction products gluconolactone and hydrogen peroxide are present; this conversion of Cr(VI) is stimulated by organic acids in a reaction that generates hydroxyl radicals, which may involve the formation of an intermediate peroxichromate(V) complex. These findings indicated that fungal glucose oxidase acts an indirect chromate reductase through the formation of Cr(VI) reducing molecules, which interact cooperatively with other fungal metabolites in the biotransformation of Cr(VI)., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
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