18 results on '"P. Molina Aguilar"'
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2. El sujeto psicológico y la microgénesis: aportes de Bärbel Inhelder al estudio de la diversidad funcional intelectual.
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Molina Aguilar, Jorge
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INTELLECTUAL development ,COGNITIVE development ,WOMEN in science ,INTELLECTUAL disabilities ,SOCIAL services ,THEORY of knowledge - Abstract
Copyright of Teoría y Praxis is the property of Editorial Universidad Don Bosco and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
- Full Text
- View/download PDF
3. Comparative measurement of collagen bundle orientation by Fourier analysis and semiquantitative evaluation: reliability and agreement in Masson's trichrome, Picrosirius red and confocal microscopy techniques
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P. Molina Aguilar, Amparo Ruiz-Sauri, Víctor Marcos-Garcés, M. Harvat, and A. Ferrández Izquierdo
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0301 basic medicine ,Polarized light microscopy ,Histology ,Materials science ,030102 biochemistry & molecular biology ,Coefficient of variation ,Analytical chemistry ,Magnification ,Pathology and Forensic Medicine ,Staining ,Masson's trichrome stain ,03 medical and health sciences ,symbols.namesake ,030104 developmental biology ,Trichrome ,Fourier analysis ,Microscopy ,symbols ,Biomedical engineering - Abstract
Summary Measurement of collagen bundle orientation in histopathological samples is a widely used and useful technique in many research and clinical scenarios. Fourier analysis is the preferred method for performing this measurement, but the most appropriate staining and microscopy technique remains unclear. Some authors advocate the use of Haematoxylin-Eosin (H&E) and confocal microscopy, but there are no studies comparing this technique with other classical collagen stainings. In our study, 46 human skin samples were collected, processed for histological analysis and stained with Masson's trichrome, Picrosirius red and H&E. Five microphotographs of the reticular dermis were taken with a 200× magnification with light microscopy, polarized microscopy and confocal microscopy, respectively. Two independent observers measured collagen bundle orientation with semiautomated Fourier analysis with the Image-Pro Plus 7.0 software and three independent observers performed a semiquantitative evaluation of the same parameter. The average orientation for each case was calculated with the values of the five pictures. We analyzed the interrater reliability, the consistency between Fourier analysis and average semiquantitative evaluation and the consistency between measurements in Masson's trichrome, Picrosirius red and H&E-confocal. Statistical analysis for reliability and agreement was performed with the SPSS 22.0 software and consisted of intraclass correlation coefficient (ICC), Bland-Altman plots and limits of agreement and coefficient of variation. Interrater reliability was almost perfect (ICC > 0.8) with all three histological and microscopy techniques and always superior in Fourier analysis than in average semiquantitative evaluation. Measurements were consistent between Fourier analysis by one observer and average semiquantitative evaluation by three observers, with an almost perfect agreement with Masson's trichrome and Picrosirius red techniques (ICC > 0.8) and a strong agreement with H&E-confocal (0.7 0.8), better with Fourier analysis than with semiquantitative evaluation (single and average). These results in nonpathological skin samples were also confirmed in a preliminary analysis in eight scleroderma skin samples. Our results show that Masson's trichrome and Picrosirius red are consistent with H&E-confocal for measuring collagen bundle orientation in histological samples and could thus be used indistinctly for this purpose. Fourier analysis is superior to average semiquantitative evaluation and should keep being used as the preferred method.
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- 2017
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4. Poster session: Aortic stenosis
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R. Piccolo, J. Clarke, C. A. Brambila, B. Igual Munoz, K. Hristova, M. S. Carvalho, M. Tesic, O. Azevedo, J. A. Del Prado, A. Mcculloch, O. Kaitozis, B. Popovic, S. Stankovic, H. Chamsi-Pasha, R. Abdelfatah, V. Parisi, K. Pushparajah, E. Zemtsovsky, B. Kilickiran Avci, A. Manouras, K. Takenaka, F. Parthenakis, P. Vardas, A. Goudev, M. Orii, A. Kutarski, R. De Rosa, M. Castillo Orive, A. Sahlen, H. Ahn, S. Nedjati-Gilani, G. J. King, H. Bellsham-Revell, D. Lahidheb, M. Anastasiou-Nana, F. Pereira Machado, S. Yurdakul, N. Olsen, S. Pica, A. Ebihara, T. Nakajima, P. Molina Aguilar, R. Hornsten, M. Elnoamany, M. Cramer, G. Tamborini, G. Pagano, H. Kim, S. Soderberg, A. M. Gonzalez, N. Zlatareva, E. Marangio, F. Yang, G. Cho, I. Paunovic, C. Jons, T. Tanimoto, H. Triantafyllidi, D. Gopalan, O. Ozcan, M. Norman, G. Grazioli, F. Castillo, E. Kort, R. Bruno, J. Kostic, M. Daimon, D. Kang, C. Badiu, C. Magnino, C. Bucca, I. Joao, F. Buendia Sanchez, A. Tomaszewski, M. Alasnig, J. Kisslo, T. Kawata, S. Fernandez Casares, A. Livingston, J. Silva Cardoso, S. Korkmaz, J. Rodriguez Garcia, M. Tomaszewski, Y. Motoyoshi, A. Kaneva, E. Kinova, J. Lekakis, N. Bruun, M. Elneklawy, K. Uno, K. Nour, J. M. Ferrer, T. Wada, T. Katova, E. Ermis, F. Gaita, S. Rafla, F. Macedo, S. Woo, S. Perry, M. Lonnebakken, K. Thapa, M. Banovic, C. Selton-Suty, V. Pereira, A. Lourenco, G. Dreyfus, W. Serra, M. Hedstrom, A. Hagendorff, H. Nishino, T. Filali, M. Muratori, F. De Stefano, J. Marin, B. Jedaida, I. Rangel, J. Haertel, S. Tzortzis, A. Kalogerakis, G. Galasso, P. Hoffman, L. Chen, Y. Juilliere, V. Kostova, J. Navarro Manchon, C. J. Lopez-Guarch, J L Moya Mur, J. D. J. Baguda, C. Moretti, C. Manisty, N. Hajlaoui, H. Mahfoudhi, E. Martins, F. Bourlon, Y. Choi, C. Papadopoulos, A. Santos, I. V. Vassiliadis, A. Pereira, D. Domingo Valero, P. Iacotucci, C. Fernandez-Golfin, P. Li, I. Xanthopoulou, G. Pontone, R. Tan, D. D. Valero, D. Cramariuc, D. Lovric, F. Maffessanti, V. Pehar Pejcinovic, Y. Xu, M. Gurzun, L. Mitrofanova, P. Sousa, M. Miglioranza, A. Goncalves, I. Nedeljkovic, S. Stanic, C Di Mario, Y. Shiono, Y. Bian, E. Tossavainen, N. Risum, L. Sargento, K. Hirata, K. Said, H. Park, A. M. Argudo, T. Kubo, S. Barker, A. Chetta, R. Palma Reis, E. Malev, C. Yao, I. Papadakis, R. Medeiros, J. Tong, M. Previtali, T. Yamaguchi, S.-H. Shin, M. Sitges, C. Calinescu, J. Rueda Soriano, K. Steine, R. Ichikawa, K. Farouk, S. Pedri, J. Ripsweden, S. Carillo, G. Gelbrich, P. Rees, F. Costantino, S. Hutchings, A. Bel Minguez, A. Gaspar, M. Petrovic, M. Li Kam Wa, E. Mavronasiou, R. Winter, I. Quelhas, J. Johnson, A. Gopal, H. Jurin, R. Rordorf, M. Al-Mallah, A. Kydd, M. Ezat, A. M. Duncan, A. Kyriacou, Y. Kim, D. Mihalcea, J. Lessa, L. Mont, T. Fritz Hansen, J. Separovic Hanzevacki, D. Mesa, R. Mincu, G. Pavlidis, A.D.J. Ten Harkel, L. Gabrielli, F. Civaia, B. Vujisic-Tesic, M. Lourenco, C. Cefalu, C. Alexandrescu, L. Stefani, D. Gerede, M. Bartesaghi, C. Calin, F. Alamanni, A. Giesecke, P. Fazendas, C. Sousa, C. Ginghina, J. Magne, S. Lemoine, M. Gonzalez, C. Gohlke-Baerwolf, K. H. Hirata, S. Fawzi, H. Kisacik, B. Popescu, L. Visconti, W. Brzozowski, M. Driessen, V. Schiano Lomoriello, S. Yamada, I. Machado, F. Silveira, A. Nordin, E. Velazquez, J. Simpson, D. Vasilev, R. Rimbas, R. Murphy, C. Szymanski, T. Imanishi, M. Martirosyan, E. Najjar, J. Chambers, I. Jovanovic, A. Nagorni, E. Gunyeli, M. Omelchenko, P. De Araujo Goncalves, E. Avenatti, R. Marinov, A. Rieck, C. Tribouilloy, I. Sitges, P. Navas Tejedor, N. Lousada, W. Fehri, B. Pezo Nikolic, T. Leiner, C. Lazaro Rivera, H. Pereira, M. Loeffler, R. Hural, D. Caldeira, D. Francis, M. Di Natale, P. Salgado Filho, F. Gao, C. Alm, G. Tarsia, A. Aleixo, D. Vinereanu, C. Cotrim, M. Lotfi, B. Mc Loughlin, H. Morita, S. K. Saha, A. Djordjevic-Dikic, D. Voilliot, R. Camporotondo, J. Shin, P. Pavlov, M. A. Cattabiani, G. Sekita, A. Djordjevic Dikic, K. Ishibashi, C. Pare, J. Kwan, S. Miyazaki, V. Di Tante, E. Svenungsson, V. Giga, Y. Ino, M. Rover, J. Niewiadomska, M. Florescu, I. Skjoerten, C. Wilson, P. Davlouros, M. Hazekamp, N. Moat, A. Correia, C. Tekedis, I. Ikonomidis, B. Dilekci, L. Magda, T. Le, D. Sohn, S. Hamdy, M. Cinteza, R. Enache, A. Milan, R. Dahmani, A. Lopez Granados, J. Zamorano Gomez, E. Zorio Grima, S. Ghulam Ali, B. Demirkan, A. Shehata, M. Vono, M. Chiarlo, Miguel Mota Carmo, D. Trifunovic, B. Bijnens, Y. Yatomi, J J Jimenez Nacher, B. Rogge, R. Nagai, D. Dutka, X. Shen, I. Mordi, M. Henein, F. Celeste, G. Nadais, H. El Atroush, T. Yamano, D. Andreini, B. Beleslin, H. Suzuki, L. Yan, S. Ghio, C. C. De Sousa, S. Stoebe, S. Petrovic-Nagorni, D. Leosco, T. Komori, S. El-Tobgi, S. Mihaila, A. Madureira, T. Leiria, G. Kim, H. Haouala, B. Stuart, G. Touati, K. Oleszczak, M. Ostojic, J. Song, D. Presutti, A. Fournier, H. Daida, M. Perez Guillen, I. Kuipers, H. Hwang, B. Belesiln, K. Park, Y. Guray, D. Pfeiffer, C. Reverberi, A. Lech, A. Valentini, A. Cogo, F. Piscione, S. Negrea, S. Mezghani, V. Pilosoff, P. Sogaard, N. Blom, N. Tzemos, A. Mantovani, K. Okada, A. Turco, M. Peltier, B. Lopez Melgar, U. Guray, Q. Chen, S. Chamuleau, T. Stanton, F. Baeza, S. M. Rafla, J. Roquette, I. Almuntaser, E. Picano, D. Rusinaru, R. Kalil, R. Martin Asenjo, A. Kiotsekoglou, A. Chilingaryan, B. Candemir, P. Sonecki, A. Moulias, M. Rosca, H. Marques, A. Patrianakos, S. Sahin, J. Estornell Erill, O. Enescu, J. Spratt, P. Barbier, M. Maciel, I. Ivanac Vranesic, P. Lindqvist, T. Snow, J. Silva-Cardoso, N. Koutsogiannis, D. Ardissino, L. Zhong, K. Adamyan, L. Mccormick, A. Calin, P. Innelli, S. Yokoyama, C. Erol, P. Pabari, A. Tarr, M. Galderisi, S. Govind, B. Suran, I. Simova, E. Guyeli, T. Pinho, L. Bjornadal, B. Diaz Anton, J. Hilde, R. Sicari, C. Beladan, M. Ege, A. Zacharaki, L. Ghiadoni, A. A. La Huerta, S. Zdravkovic-Ciric, O. Huttin, K. Jensen-Urstad, F. Veglio, M. Elsedi, M. Nakabachi, P. Zinzius, D. Kim, H. Dores, A. Kakkavas, H. Badran, V. Sanchez Sanchez, E. Duo, J. Carrasco, A. Almeida, M. Virdee, M. Llemit, A. Anwar, L. Pratali, J. Monmeneu Menadas, S. Nevin, L. Fusini, F. Lombera Romero, E. Despotopoulos, E. Nyktari, G. Galanti, K. Kim, A. Van Der Hulst, H. Khachab, M. Dikic, I. Cruz, M. Melsom, J. Brugada, V. Mitic, M. Landolina, S. Turhan, V. Hansteen, D Rodriguez Munoz, J. S. De Lezo, N. Gori, Z. Baricevic, S.-P. Lee, M. Arnau Vives, S. Lee, P. Gripari, S. Humerfelt, F. Huang, T. Mikami, G. Soltan, T. Akasaka, S. Kaga, G. Penney, L. Toncelli, K. Boman, B. Basnyat, E. Kowalik, A. Bartolini, S. Georgiev, K. Shahgaldi, M. Pepi, M. Ruiz Ortiz, R. Sant'anna, H. Tsutsui, P. A. Fernandez, G. Tempesti, S. Aytekin, H. Iwano, Y. Nosir, C. Raineri, J. Rasmunsson, S. Lasarov, P. Lopez Lereu, V. Persic, F. Khan, J. Hisdal, M. Gommidh, A. Alhagoly, E. Gerdts, M. Milicia, G. Rengo, K. Kimura, F. Hakansson, M. Morenate, P. Mitev, M. Yacoub, M. Satendra, B. Kusmierczyk-Droszcz, E. Romo, R. Jankovic-Tomasevic, A. Roest, J. Stepanovic, J. Schwartz, Z. Ashour, L. Klitsie, J. Giner Blasco, M. Delgado, P. Omede, S. Mayordomo Gomez, I. Paraskevaidis, J. L. Zamorano, N. Goodfield, E. Dores, S. Davies, N. Patrascu, D. Alexopoulos, L. Donate Bertolin, D. Stanojevic, E. Psathakis, M. Dobric, P. Trivilou, H. Sasmaz, A. Marinkovic, O. Mirea, G. Sieswerda, M. Maruyama, A. M. Maceira Gonzalez, T. I. Imanishi, A. Santoro, G. Festa, R. Coma Samartin, and V. Atanaskovic
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medicine.medical_specialty ,Stenosis ,business.industry ,Internal medicine ,Cardiology ,medicine ,Radiology, Nuclear Medicine and imaging ,General Medicine ,Session (computer science) ,Cardiology and Cardiovascular Medicine ,business ,medicine.disease - Published
- 2012
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5. Clinical Exome Sequencing Enables Congenital Sialidosis Type II Diagnosis in Two Siblings Presenting with Unreported Clinical Features from a Rare Homozygous Sequence Variant p.(Tyr370Cys) in NEU1
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Flores-Contreras, Elda Ariadna, García-Ortiz, José Elías, Robles-Espinoza, Carla Daniela, Zomosa-Signoret, Viviana, Becerra-Solano, Luis Eduardo, Vidaltamayo, Román, Castaneda-García, Carolina, Esparza-García, Eduardo, Molina-Aguilar, Christian, Hernández-Orozco, Angélica Alejandra, and Córdova-Fletes, Carlos
- Abstract
Sialidosis is a rare autosomal recessive disease that presents with progressive lysosomal storage of sialylated glycopeptides and oligosaccharides caused by homozygous or compound heterozygous sequence variants in the neuraminidase 1 (NEU1) gene. These sequence variants can lead to sialidosis type I and II; the latter is the most severe and presents prenatally or at early age. However, sialidosis diagnosis is challenging, especially in those health systems with limited resources of developing countries. Consequently, it is necessary to dip into high-throughput molecular diagnostic tools to allow for an accurate diagnosis with better cost-effectiveness and turnaround time. We report a 4-member pedigree segregating an ultrarare missense variant, c.1109A>G; p.Tyr370Cys, in NEU1as detected by whole-exome sequencing. Two short-lived siblings, who presented with previously unreported clinical features from such a homozygous sequence variant, were diagnosed with sialidosis type II. Additionally, we present a novel molecular model exhibiting the consequences of the variant in the sialidase-1 tridimensional structure. This study allowed us to provide a definitive diagnosis for our patients, increase our understanding of this pathogenic variant, and improve genetic counseling.
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- 2021
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6. Fruta fresca, cuerpos marchitos. Trabajadores agrícolas migrantes en Estados Unidos.
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Molina Aguilar, Jorge
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- 2020
7. Correlación canónica entre volúmenes de almacenamiento en presas e intensidades de precipitación durante huracanes.
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Pablo Molina-Aguilar, Juan, Gutiérrez-López, Alfonso, and Cruz Paz, Ivonne Monserrat
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HYDRAULICS ,DAMS ,HURRICANES ,METEOROLOGICAL precipitation ,RAINFALL ,MEASUREMENT of runoff - Abstract
Copyright of Tecnología y Ciencias del Agua is the property of Instituto Mexicano de Tecnologia del Agua (IMTA) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2019
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- View/download PDF
8. Sudden Death Due to Thoracic Aortic Dissection in Young People: A Multicenter Forensic Study.
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Morentin Campillo, Benito, Molina Aguilar, Pilar, Monzó Blasco, Ana, Laborda Gálvez, José Luis, Arrieta Pérez, Jon, Sancho Jiménez, Jennifer, Lamas Ruiz, Julia, and Lucena Romero, Joaquín
- Abstract
Copyright of Revista Española de Cardiología (18855857) is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
- Full Text
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9. Age-related dermal collagen changes during development, maturation and ageing - a morphometric and comparative study
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V. García Bustos, Víctor Marcos-Garcés, C. Bea Serrano, P. Molina Aguilar, J. Benavent Seguí, Amparo Ruiz-Sauri, and A. Ferrández Izquierdo
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Adult ,Male ,medicine.medical_specialty ,Histology ,Adolescent ,Human skin ,Masson's trichrome stain ,Young Adult ,Internal medicine ,Linear regression ,medicine ,Image Processing, Computer-Assisted ,Humans ,Child ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Aged ,Polynomial regression ,Aged, 80 and over ,Chemistry ,Papillary dermis ,Infant ,Regression analysis ,Cell Biology ,Anatomy ,Dermis ,Original Articles ,Middle Aged ,Skin Aging ,Endocrinology ,Ageing ,Child, Preschool ,Regression Analysis ,Female ,Collagen ,Reticular Dermis ,Developmental Biology - Abstract
The tissue organisation of dermal collagen is gaining importance as a contributing factor both in development and ageing, as well as in skin maturation processes. In this work we aim to study different representative parameters of this structural organisation in 45 human skin samples of assorted ages, by means of image analysis. The variation of these parameters on the basis of age was assessed using several regression models (linear, quadratic and cubic). The area occupied by collagen was significantly reduced as a function of age in the papillary dermis (R(2) = 0.437, P < 0.0001), as well as the thickness of the collagen bundles (R(2) = 0.461, P < 0.0001), following statistical models of cubic and quadratic regression, respectively. The width of the papillary dermis increased in a significant manner over a linear regression model (R(2) = 0.26, P < 0.0001). In the reticular dermis, the cubic regression indicated a significant decline (R(2) = 0.392, P = 0.002) of the area filled with collagen according to the age. Both collagen thickness and bundle orientation parameters fit a quadratic regression over the age in a significant way (R(2) = 0.433 and R(2) = 0.334, respectively, both P < 0.0001). The width of the reticular dermis followed also a significant quadratic distribution according to age (R(2) = 0.193, P = 0.011). These parameters could partially explain the lifelong functional changes taking place in the skin and propose a baseline providing a useful entry point for future investigation.
- Published
- 2014
10. Búsqueda armónica para optimizar la función Gumbel Mixta univariada.
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Pablo Molina-Aguilar, Juan, Alfonso Gutiérrez-López, Martín, and Javier Aparicio-Mijares, Francisco
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STANDARD deviations ,DISTRIBUTION (Probability theory) ,ALGORITHMS ,SEARCH algorithms ,PARAMETER estimation - Abstract
Copyright of Tecnología y Ciencias del Agua is the property of Instituto Mexicano de Tecnologia del Agua (IMTA) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2018
- Full Text
- View/download PDF
11. Muerte súbita por disección de aorta torácica en jóvenes: estudio multicéntrico forense
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Morentin Campillo, Benito, Molina Aguilar, Pilar, Monzó Blasco, Ana, Laborda Gálvez, José Luis, Arrieta Pérez, Jon, Sancho Jiménez, Jennifer, Lamas Ruiz, Julia, and Lucena Romero, Joaquín
- Abstract
La disección de la aorta torácica (DAT) es infrecuente en jóvenes y presenta características diferentes que en la población adulta. En este estudio se analizan las características clinicopatológicas de la muerte súbita por DAT de personas de 1-35 años.
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- 2019
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12. Patología coronaria no arteriosclerótica como causa de muerte súbita en adultos: Casuística del Instituto de Medicina Legal de Valencia (1997-2005)
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F. Carratalá Calvo, P. Molina Aguilar, C. de la Presentación Blasco, and Jm. Ortiz Criado
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Enfermedad coronaria ,business.industry ,muerte súbita cardiaca ,Medicine ,business ,patología forense ,Pathology and Forensic Medicine - Abstract
Introducción y objetivos. En los países industrializados, la causa más frecuente de muerte súbita cardíaca (MSC) en el adulto es la isquemia miocárdica aguda por arterioesclerosis coronaria. Sin embargo, existen otras patologías coronarias, congénitas o adquiridas, que pueden producir isquemia miocárdica y muerte súbita: origen anómalo, hipoplasia, estenosis del ostium, aneurisma, disección, localización alta del ostium, puente miocárdico, vasculitis. El objetivo del presente trabajo es revisar la casuística de este tipo de patología coronaria no arterioesclerótica en las 8003 autopsias realizadas en el Instituto de Medicina Legal de Valencia (IMLV) entre los años 1997-2005. Método: Los criterios de inclusión de los casos para este estudio han sido: edad superior a 14 años, patología coronaria no arterioesclerótica como causa de muerte y circunstancias de la muerte compatibles con la definición de muerte súbita. Resultados: Se han encontrado 9 casos de muerte súbita en adultos con patología coronaria no arterioesclerótica como causa de muerte: 2 casos de puente miocárdico, 6 casos de disección coronaria y 1 caso de origen anómalo de coronaria. Conclusiones: La patología coronaria no arterioesclerótica, aunque es poco frecuente como causa de MSC, debemos conocerla y poder diagnosticarla macroscópicamente en la sala de autopsias. Siempre debe descartarse otra posible causa de muerte mediante los estudios complementarios químico-toxicológicos e histopatológicos. Es importante conseguir la máxima información clínica anterior al fallecimiento que pueda ayudar al diagnóstico en vida de este tipo de patologías, ya que en la mayoría de los casos sigue siendo un hallazgo postmortem.
- Published
- 2005
13. Tackling the lack of diversity in cancer research
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Molina-Aguilar, Christian and Robles-Espinoza, C. Daniela
- Abstract
Despite the clear benefit of studying biological samples from diverse genetic backgrounds and geographical locations, our current knowledge of disease is mostly derived from the study of European-descent individuals. In the cancer field, this is reflected in the poor representation of African and Amerindian/Latino samples in most large public data repositories. This lack of diversity is due to several reasons, but here we focus on (1) the lack of support for studies on non-European populations that are performed in low- and middle-income countries (LMICs), and (2) unequal partnerships between scientists in LMICs and those in high-income countries. We argue that expanding access to research funding, increasing the participation of underrepresented scientists in editorial boards and international conferences, facilitating the publication of studies conducted in these countries, and properly acknowledging LMIC researchers' contributions in publications and grant applications will promote equity for scientists working in LMICs. We envisage that this will translate to more impactful research in these countries, which will include more samples from diverse populations. For the cancer field, this will broaden our understanding of pathomechanisms and may help to improve the treatment of patients from all backgrounds.
- Published
- 2023
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14. Unidades multidisciplinares en el estudio y prevención de la muerte súbita por cardiopatías familiares
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Molina Aguilar, Pilar, Giner Blasco, Juan, Izquierdo Macian, Isabel, Martínez-Dolz, Luis, Barriales Villa, Roberto, and Zorio Grima, Esther
- Abstract
La muerte súbita es fundamentalmente cardiaca y tiene un gran impacto social, económico y mediático. Cuando acontece en personas jóvenes, suele obedecer a causas genéticas (cardiopatías familiares). En este grupo se incluyen las miocardiopatías, canalopatías y el aneurisma/disección aórtica familiar. Las cardiopatías familiares son enfermedades raras en términos de prevalencia y poco conocidas, por lo que su manejo requiere formación especializada y multidisciplinar. La muerte súbita suele acontecer en el ámbito extrahospitalario y es objeto de una autopsia forense. Estas autopsias deberían realizarse de acuerdo a los estándares mínimos de calidad de las guías europeas. El claro sustrato genético justifica la necesidad de un screening familiar para realizar diagnósticos precoces y también para descartarla en otros familiares en riesgo a través de un abordaje multidisciplinar que ofrezca una medicina personalizada a las familias afectadas. Además de las cuestiones médicas, este esquema permite ofrecer apoyo psicológico precoz y consejo genético de cara a planificar nuevas gestaciones. En España existe un gran vacío legal en el abordaje de la muerte súbita y su prevención, con una falta de homogenización entre las distintas comunidades autónomas tanto a nivel judicial como asistencial debido a las características del proceso de transferencia de competencias y a la organización territorial judicial y forense. Sería deseable que, desde los órganos consultivos de ambos ministerios (Sanidad y Justicia) se elaborara un plan de actuación global que asegurara la calidad en el estudio post mortemy su continuidad en el posterior estudio familiar clínico y genético. Así, el potencial clínico-preventivo de las autopsias tomaría cuerpo y revertiría en un claro beneficio para las familias afectadas y para la sociedad en general favoreciendo asimismo la docencia y la investigación, tan importantes para el avance del conocimiento en este ámbito tan desconocido y devastador.
- Published
- 2018
- Full Text
- View/download PDF
15. Poster session Friday 13 December - AM: 13/12/2013, 08:30-12:30 * Location: Poster area
- Author
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Gertsen, M, Nemes, A, Szolnoky, G, Altmayer, A, Gavaller, H, Kemeny, L, Forster, T, Park, J R, Jo, SY, Kim, KH, Kho, JS, Kwack, CH, Hwang, JY, Popovic, D, Ostojic, MC, Petrovic, M, Vujisic-Tesic, B, Arandjelovic, A, Banovic, M, Vukcevic, V, Petrovic, I, Popovic, B, Damjanovic, S, Placido, R, Marta, L, Ramalho, AR, Nobre Menezes, M, Cortez-Dias, N, Martins, S, Goncalves, S, Almeida, AG, Silva-Marques, J, Nunes-Diogo, A, Germanakis, I, Kakouri, P, Karachaliou, M, Vassilaki, M, Chatzi, L, Roumeliotaki, T, Kogevinas, M, Horst, J-P, Kelter-Kloepping, A, Koerperich, H, Barth, P, Haas, NA, Kececioglu, D, Laser, KT, Laser, KT, Horst, J-P, Kelter-Kloepping, A, Barth, P, Haas, NA, Kececioglu, D, Koerperich, H, Samiei, N, Nabati, M, Azari-Jafari, M, Vakili-Zarch, A, Parsaee, M, Haghjoo, M, Ahmed, A J, Val-Mejias, J E, Von Bulow, F M, Baltussen, E J M, Darban, AM, Claus, P, Voigt, JU, Rodriguez Munoz, DA, Moya Mur, JL, Gonzalez, A, Garcia Martin, A, Becker Filho, D, Fernandez Santos, S, Lazaro Rivera, C, Recio Vazquez, M, Fernandez Golfin, C, Zamorano Gomez, JL, Bandera, F, Pellegrino, M, Generati, G, Alfonzetti, E, Donghi, V, Castelvecchio, S, Garatti, A, Menicanti, L, Guazzi, M, Kowalik, E, Klisiewicz, A, Hoffman, P, Kim, EJ, Cho, I J, Oh, J, Chang, HJ, Park, J, Shin, S, Shim, CY, Hong, GR, Ha, JW, Chung, N, Park, JH, Lee, HS, Kim, HS, Ahn, KT, Kim, JH, Lee, JH, Choi, SW, Jeong, JO, Seong, IW, Holzendorf, V, Gelbrich, G, Wachter, R, Loeffler, M, Pieske, BM, Broda, A, Edelmann, F, Failure, German Competence Network for Heart, Kim, YH, Kim, DH, Kim, SH, Ahn, JC, Song, WH, Hashimoto, G, Suzuki, M, Yoshikawa, H, Otsuka, T, Kusunose, Y, Nakamura, M, Sugi, K, De Knegt, M C, Biering-Sorensen, T, Sogaard, P, Sivertsen, J, Jensen, JS, Mogelvang, R, Murbraech, K, Smeland, KH, Holte, H, Loge, JH, Kiserud, CE, Aakhus, S, Peteiro, J, Gargallo-Fernandez, P, Garcia-Guimaraes, M, Bouzas-Mosquera, A, Yanez-Wronenburger, JC, Martinez-Ruiz, D, Castro-Beiras, A, Trzcinski, PT, Jaskowski, MJ, Nowak, JN, Pawlus, MP, Figiel, LF, Kasprzak, JDK, Lipiec, PL, Zhong, L, Su, Y, Teo, SK, Le, TT, Tan, RS, Tesic, M, Djordjevic-Dikic, A, Giga, V, Jovanovic, I, Paunovic, I, Petrovic, MT, Trifunovic, D, Beleslin, B, Stepanovic, J, Vujisic-Tesic, B, Parato, V M, Partemi, M, Nardini, E, Pasanisi, E, Park, T-H, Lee, J-E, Lee, D-H, Park, J-S, Park, K, Kim, M-H, Kim, Y-D, Vegsundvag, J, Holte, E, Wiseth, R, Hegbom, K, Hole, T, Fusini, L, Tamborini, G, Ghulam Ali, S, Muratori, M, Gripari, P, Cefalu, C, Maffessanti, F, Celeste, F, Alamanni, F, Pepi, M, Negrea, SL, Alexandrescu, C, Rossi, P, Iacuzio, L, Dreyfus, G, Moatemri, F, Mahdhaoui, A, Bouraoui, H, Ernez, S, Jeridi, G, Yuan, L, Feng, JL, Jin, X Y, Seoane Garcia, T, Delgado Ortega, M, Mesa Rubio, D, Ruiz Ortiz, M, Martin Hidalgo, M, Carrasco Avalos, F, Casares Mediavilla, J, Alados, P, Lopez Granados, A, Suarez De Lezo Cruz Conde, J, Mutuberria Urdaniz, M, Rodriguez-Palomares, JF, Baneras-Rius, JF, Acosta-Velez, JG, Buera-Surribas, I, Gonzalez-Alujas, MT, Teixido, G, Evangelista, A, Tornos, P, Garcia-Dorado, D, Iliuta, L, Boerlage-Van Dijk, K, Van Riel, ACMJ, De Bruin-Bon, HACM, Wiegerinck, EMA, Koch, KT, Vis, MM, Meregalli, PG, Piek, JJ, Bouma, BJ, Baan, J, Enache, R, Muraru, D, Piazza, R, Popescu, BA, Coman, M, Calin, A, Rosca, M, Beladan, CC, Nicolosi, GL, Ginghina, C, Song, JM, Kim, JJ, Ha, TY, Jung, SH, Hwang, IS, Lee, IC, Sun, BJ, Kim, DH, Kang, DH, Song, JK, Sturmberger, T, Ebner, CE, Aichinger, J, Tkalec, W, Niel, J, Steringer-Mascherbauer, R, Kabicher, G, Winter, S, Nesser, HJ, Hofmann-Bowman, M, Lin Yan, LY, Puri, TP, Chin, C W L, Doris, M, Shah, A, Mills, N, Semple, S, Prasad, S, White, A, Dweck, M, Newby, D, Debonnaire, P, Al Amri, I, Leong, DP, Joyce, E, Katsanos, S, Kamperidis, V, Schalij, MJ, Bax, JJ, Ajmone Marsan, N, Delgado, V, Cerin, G, Popa, B A, Lanzillo, G, Benea, D, Karazanishvili, L, Diena, M, Dedobbeleer, C, Schnell, F, Jotrand, E, El Mourad, M, Thebault, C, Plein, D, Donal, E, Unger, P, Spampinato, RA, Tasca, M, Da Rocha E Silva, JG, Strotdrees, E, Schloma, V, Dmitrieva, Y, Mende, M, Borger, MA, Mohr, FW, Veronesi, F, Muraru, D, Addetia, K, Corsi, C, Lamberti, C, Lang, RM, Mor-Avi, V, Badano, LP, Zemanek, D, Tomasov, P, Belehrad, M, Kara, T, Veselka, J, Igual Munoz, B, Estornell Erill, JORDI, Maceira Gonzalez Alicia, AMG, Monmeneu Menadas, JVMM, Lopez Lereu Pilar, PLL, Molina Aguilar, PMA, Domingo-Valero, DDV, Osca Asensi, JOA, Zorio Grima, EZG, Salvador Sanz Antonio, ASS, Ibrahimi, P, Bajraktari, G, Poniku, A, Hysenaj, V, Ahmeti, A, Jashari, F, Haliti, E, Henein, MY, Maramao, F, Conde, Y, Maramao, L, Rulli, F, Roussin, I, Drakopoulou, M, Bhattacharyya, S, Simpkin, V, Sharma, R, Rosen, S, Prasad, S, Senior, R, Lyon, AR, Kimura, K, Tanimoto, T, Akasaka, T, Fijalkowski, M, Jaguszewski, M, Fijalkowska, M, Nowak, R, Galaska, R, Rojek, A, Narkiewicz, K, Rynkiewicz, A, Azevedo, O, Marques, N, Cruz, I, Picarra, B, Lima, R, Amado, J, Pereira, V, Almeida, AR, SUNSHINE, Zito, C, Crea, P, Cusma Piccione, M, Vriz, O, Bitto, A, Minisini, R, Madaffari, A, Acri, E, Oteri, A, Carerj, S, Leggio, S, Buccheri, S, Tamburino, C, Monte, I P, Mihalcea, D, Florescu, M, Enescu, OA, Magda, LS, Radu, E, Acasandrei, AM, Balanescu, P, Rimbas, RC, Jinga, D, Vinereanu, D, 112/2011, Research grant, Miyoshi, T, Tanaka, H, Kaneko, A, Matsumoto, K, Imanishi, J, Motoji, Y, Mochizuki, Y, Minami, H, Kawai, H, Hirata, K, Ryu, SK, Shin, DG, Son, JW, Choi, JH, Goh, CW, Choi, JW, Park, JY, Hong, GR, Le Page, P, Mitchell, ARJ, Maclachlan, HI, Hurry, RW, Villagraz Tecedor, L, Jimenez Lopez Guarch, C, Alonso Chaterina, S, Mayordomo Gomez, S, Blazquez Arrollo, L, Lombera Romero, F, Lopez Melgar, B, Escribano Subias, MP, Lichodziejewska, B, Kurnicka, K, Goliszek, S, Kostrubiec, M, Dzikowska Diduch, O, Krupa, M, Grudzka, K, Ciurzynski, M, Palczewski, P, Pruszczyk, P, Lovric, D, Carmona, C, Bergerot, C, Schnell, F, Thibault, H, Barthelet, M, Ninet, J, Revel, D, Croisille, P, Derumeaux, G, Jensen, MT, Rossing, P, Sogaard, P, Andersen, HU, Bech, J, Hansen, TF, Gustafsson, I, Galatius, S, Jensen, JS, Shang, Q, Zhang, Q, Sanderson, JE, Tam, LS, Lee, A PW, Fang, F, Li, E KM, Yu, CM, Bruin De- Bon, HACM, Tan, HL, Hardziyenka, M, Symersky, P, Bonta, PI, Brink Van Den, RBA, Bouma, BJ, Bader, RS, Punn, R, Silverman, N, Cruz, C, Pinho, T, Lebreiro, A, Dias, CC, Silva Cardoso, J, Julia Maciel, M, Melao, F, Ribeiro, V, Cruz, C, Maciel, MJ, Attenhofer Jost, C H, Schmidt, D, Pfyffer, M, Biaggi, P, Seifert, B, Weber, R, De Pasquale, G, Kretschmar, O, Seeliger, T, Greutmann, M, Johansson, M C, Mirzada, N, Ladenvall, P, Besiroglu, F, Samadov, F, Atas, H, Sari, I, Tufekcioglu, O, Birincioglu, CL, Acar, B, Duman, I, Colak, A, Zagatina, A, Krylova, L, Zhuravskaya, N, Vareldzhyan, Y, Tyurina, TV, Clitsenko, O, Castro, M, Dores, H, Carvalho, MS, Reis, C, Horta, E, Trabulo, MS, Andrade, MJ, Mendes, M, Gasior, Z, Plonska-Gosciniak, E, Wita, K, Mizia-Stec, K, Kulach, A, Szwed, H, Chrzanowski, L, Tomaszewski, A, Sinkiewicz, W, Wojciechowska, C, Aggeli, C, Felekos, I, Stergiou, P, Roussakis, G, Kakiouzi, V, Kastellanos, S, Koutagiar, I, Stefanadis, C, Bouzas Mosquera, A, Peteiro, J, Alvarez-Garcia, N, Broullon, FJ, Garcia-Guimaraes, MM, Martinez-Ruiz, D, Yanez-Wonenburger, JC, Bouzas-Zubeldia, B, Fabregas, R, Castro-Beiras, A, Brugger, N, Huerzeler, M, Wustmann, K, Wahl, A, Steck, H, Seiler, C, Sarwar, R, Malhotra, A, Wong, KC, Betts, TR, Bashir, Y, Rajappan, K, Newton, JD, Casanova Rodriguez, C, Cano Carrizal, R, Iglesias Del Valle, D, Martin Penato Molina, A, Garcia Garcia, A, Prieto Moriche, E, Alvarez Rubio, J, Paredes Gonzalez, B, De Juan Baguda, J, Plaza Perez, I, Van Den Oord, SCH, Akkus, Z, Roeters Van Lennep, JE, Bosch, JG, Van Der Steen, AFW, Sijbrands, EJG, Schinkel, AFL, Muraru, D, Calore, C, Badano, LP, Melacini, C, Mihaila, S, Peluso, D, Puma, L, Kocabay, G, Rizzon, G, Iliceto, S, Bochard Villanueva, B, Paya-Serrano, R, Garcia-Gonzalez, P, Fabregat-Andres, O, Perez-Bosca, JL, Cubillos-Arango, A, Ferrando-Beltran, M, Chacon-Hernandez, N, Albiach-Montanana, C, Ridocci-Soriano, F, Ancona, R, Comenale Pinto, S, Caso, P, Arenga, F, Coppola, MG, Calabro, R, Tarr, A, Stoebe, S, Pfeiffer, D, Hagendorff, A, Hollekim, SM, Bjorgaas, MR, Tjonna, AE, Wisloff, U, Ingul, CB, (CERG), Cardiac Exercise Research Group, Oreto, L, Zito, C, Cusma-Piccione, M, Calabro, MP, Todaro, MC, Vita, GL, Messina, S, Vita, G, Sframeli, M, Carerj, S, Remoli, R, Lamberti, F, Bellini, C, Mercurio, M, Dottori, S, Bellusci, F, Mazzuca, V, Gaspardone, A, Rimbas, RC, Enescu, OA, Mihaila, S, Ciobanu, A, Vinereanu, D, Henri, C, Magne, J, Dulgheru, R, Laaraibi, S, Voilliot, D, Kou, S, Pierard, L, Lancellotti, P, Wellnhofer, E, Kriatselis, C, Gerds-Li, H, Furundzija, VESNA, Thanabalasingam, U, Fleck, E, Graefe, M, Kouris, N, Keramida, K, Karidas, V, Kostopoulos, V, Kostakou, P, Mprempos, G, Olympios, CD, Duchateau, N, Giraldeau, G, Gabrielli, L, Penela, D, Evertz, R, Mont, L, Brugada, J, Berruezo, A, Bijnens, BH, Sitges, M, Bernard, A, Donal, E, Reynaud, A, Schnell, F, Daubert, JC, Leclercq, C, Hernandez, A, Keramida, K, Kouris, N, Kostopoulos, V, Karidas, V, Dagre, A, Ntarladimas, I, Damaskos, D, Stamatelatou, M, Olympios, CD, Panetta, G L, Peraldo Neja, C, Urbano Moral, JA, Evangelista, A, Azzolini, P, Gaudio, C, Pandian, NG, Barbier, P, Mirea, O, Savioli, G, Cefalu, C, Guglielmo, M, Fusini, L, Maltagliati, A, Hamdy, AM, Fereig, HM, Nabih, MA, Abdel-Aziz, A, Ali, AA, Buccheri, S, Mangiafico, S, Leggio, S, B, VE, Tropea, L, Tamburino, C, Monte, I P, Garcia-Gonzalez, P, Chacon-Hernandez, N, Cozar-Santiago, P, Fabregat-Andres, O, Sanchez-Jurado, R, Higueras-Ortega, L, Albiach-Motanana, C, Perez-Bosca, JL, Paya-Serrano, R, Ridocci-Soriano, F, Flori, M, Valette, F, Guijarro, D, Pallardy, A, Le Tourneau, T, Kraeber-Bodere, F, Piriou, N, Saxena, A, Ramakrishnan, S, Tulunay Kaya, C, Ongun, A, Kilickap, M, Candemir, B, Altin, AT, Gerede, M, Ozcan, OU, Erol, C, Yue, WS, Yang, F, Huang, D, Gu, P, Luo, Y, Lv, Z, Siu, CW, Tse, HF, Yiu, KH, Saura Espin, D, Lopez Cuenca, A, Espinosa Garcia, MD, Oliva Sandoval, MJ, Lopez Ruiz, M, Gonzalez Carrillo, J, Garcia Navarro, MJ, Valdes Chavarri, M, De La Morena Valenzuela, G, Gustafsson, U, Spuhler, JH, Hoffman, J, Brodin, LÅ, Kisko, A, Dernarova, L, Hudakova, A, Santova, T, Jakubikova, M, Mikulak, M, Horlenko, O, Kishko, N, Svystak, V, Shyp, A, Faden, G, Gaibazzi, N, Rigo, F, Mureddu, GF, Moreo, A, Bussadori, G, Facchetti, R, Cesana, F, Giannattasio, C, Faggiano, P, and group, APRES collaborative
- Abstract
Pulmonary vascular dysfunction is claimed to be a contributor to the development of pulmonary hypertension (PH). Impaired systemic vascular reactivity is one of the essential factors in the pathogenesis of cardiovascular disease. The aim of the investigation was to study whether there is any association between systemic vascular function and pulmonary artery pressure (PAP) in patients who have associated causes for PH development, such as coronary heart disease (CHD) and chronic obstructive pulmonary disease (COPD). Methods: The brachial artery vasodilator responses were measured by the ultrasound technique in twenty patients with mild to moderate COPD (group I) and twenty age–matched and COPD stage-matched patients who had past history of myocardial infarction (NYHA II) (group II).Conventional echocardiographic variables were measured in the said patients too. Results: Both flow-mediated dilatation (FMD) and nitrate-mediated dilatation (NMD) were significantly lower, and PAP was significantly higher in the group II patients compared to the same parameters of group I patients. NMD was inversely correlated with PAP (r=-0.7, p=0.02) in group I patients. There was no interrelation between FMD and PAP in patients from group I. Neither FMD nor NMD were correlated with PAP in group II patients. A significant positive correlation between PAP and left ventricular mass index (r=0.8, p=0.003) was revealed in the said patients as well. Conclusions: Attenuated vasodilator response of brachial artery to nitroglycerine is associated with PAP elevation in COPD patients. PH is closely related to cardiac remodeling in COPD patients in whom CHD developed. These data suggest different "stages" of vascular and cardiac remodeling in patients with COPD alone and in coexistence with CHD. The obtained data can be useful in the selection of treatment as regards these patient categories.
- Published
- 2013
- Full Text
- View/download PDF
16. Comparative measurement of collagen bundle orientation by Fourier analysis and semiquantitative evaluation: reliability and agreement in Masson's trichrome, Picrosirius red and confocal microscopy techniques.
- Author
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Marcos-Garcés V, Harvat M, Molina Aguilar P, Ferrández Izquierdo A, and Ruiz-Saurí A
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Female, Hospitals, University, Humans, Image Processing, Computer-Assisted methods, Infant, Male, Middle Aged, Skin pathology, Spain, Young Adult, Collagen analysis, Fourier Analysis, Histocytochemistry methods, Microscopy methods, Microscopy, Confocal methods, Microscopy, Polarization methods, Staining and Labeling methods
- Abstract
Measurement of collagen bundle orientation in histopathological samples is a widely used and useful technique in many research and clinical scenarios. Fourier analysis is the preferred method for performing this measurement, but the most appropriate staining and microscopy technique remains unclear. Some authors advocate the use of Haematoxylin-Eosin (H&E) and confocal microscopy, but there are no studies comparing this technique with other classical collagen stainings. In our study, 46 human skin samples were collected, processed for histological analysis and stained with Masson's trichrome, Picrosirius red and H&E. Five microphotographs of the reticular dermis were taken with a 200× magnification with light microscopy, polarized microscopy and confocal microscopy, respectively. Two independent observers measured collagen bundle orientation with semiautomated Fourier analysis with the Image-Pro Plus 7.0 software and three independent observers performed a semiquantitative evaluation of the same parameter. The average orientation for each case was calculated with the values of the five pictures. We analyzed the interrater reliability, the consistency between Fourier analysis and average semiquantitative evaluation and the consistency between measurements in Masson's trichrome, Picrosirius red and H&E-confocal. Statistical analysis for reliability and agreement was performed with the SPSS 22.0 software and consisted of intraclass correlation coefficient (ICC), Bland-Altman plots and limits of agreement and coefficient of variation. Interrater reliability was almost perfect (ICC > 0.8) with all three histological and microscopy techniques and always superior in Fourier analysis than in average semiquantitative evaluation. Measurements were consistent between Fourier analysis by one observer and average semiquantitative evaluation by three observers, with an almost perfect agreement with Masson's trichrome and Picrosirius red techniques (ICC > 0.8) and a strong agreement with H&E-confocal (0.7 < ICC < 0.8). Comparison of measurements between the three techniques for the same observer showed an almost perfect agreement (ICC > 0.8), better with Fourier analysis than with semiquantitative evaluation (single and average). These results in nonpathological skin samples were also confirmed in a preliminary analysis in eight scleroderma skin samples. Our results show that Masson's trichrome and Picrosirius red are consistent with H&E-confocal for measuring collagen bundle orientation in histological samples and could thus be used indistinctly for this purpose. Fourier analysis is superior to average semiquantitative evaluation and should keep being used as the preferred method., (© 2017 The Authors Journal of Microscopy © 2017 Royal Microscopical Society.)
- Published
- 2017
- Full Text
- View/download PDF
17. Age-related dermal collagen changes during development, maturation and ageing - a morphometric and comparative study.
- Author
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Marcos-Garcés V, Molina Aguilar P, Bea Serrano C, García Bustos V, Benavent Seguí J, Ferrández Izquierdo A, and Ruiz-Saurí A
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Female, Humans, Image Processing, Computer-Assisted, Infant, Male, Middle Aged, Regression Analysis, Young Adult, Collagen analysis, Dermis cytology, Skin Aging pathology
- Abstract
The tissue organisation of dermal collagen is gaining importance as a contributing factor both in development and ageing, as well as in skin maturation processes. In this work we aim to study different representative parameters of this structural organisation in 45 human skin samples of assorted ages, by means of image analysis. The variation of these parameters on the basis of age was assessed using several regression models (linear, quadratic and cubic). The area occupied by collagen was significantly reduced as a function of age in the papillary dermis (R(2) = 0.437, P < 0.0001), as well as the thickness of the collagen bundles (R(2) = 0.461, P < 0.0001), following statistical models of cubic and quadratic regression, respectively. The width of the papillary dermis increased in a significant manner over a linear regression model (R(2) = 0.26, P < 0.0001). In the reticular dermis, the cubic regression indicated a significant decline (R(2) = 0.392, P = 0.002) of the area filled with collagen according to the age. Both collagen thickness and bundle orientation parameters fit a quadratic regression over the age in a significant way (R(2) = 0.433 and R(2) = 0.334, respectively, both P < 0.0001). The width of the reticular dermis followed also a significant quadratic distribution according to age (R(2) = 0.193, P = 0.011). These parameters could partially explain the lifelong functional changes taking place in the skin and propose a baseline providing a useful entry point for future investigation., (© 2014 Anatomical Society.)
- Published
- 2014
- Full Text
- View/download PDF
18. The adult macaque spinal cord central canal zone contains proliferative cells and closely resembles the human.
- Author
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Alfaro-Cervello C, Cebrian-Silla A, Soriano-Navarro M, Garcia-Tarraga P, Matías-Guiu J, Gomez-Pinedo U, Molina Aguilar P, Alvarez-Buylla A, Luquin MR, and Garcia-Verdugo JM
- Subjects
- Adult, Age Factors, Aged, Aged, 80 and over, Animals, Epithelial Cells physiology, Female, Humans, Macaca, Macaca fascicularis, Male, Species Specificity, Spinal Canal ultrastructure, Spinal Cord ultrastructure, Cell Proliferation physiology, Spinal Canal cytology, Spinal Canal physiology, Spinal Cord cytology, Spinal Cord physiology
- Abstract
The persistence of proliferative cells, which could correspond to progenitor populations or potential cells of origin for tumors, has been extensively studied in the adult mammalian forebrain, including human and nonhuman primates. Proliferating cells have been found along the entire ventricular system, including around the central canal, of rodents, but little is known about the primate spinal cord. Here we describe the central canal cellular composition of the Old World primate Macaca fascicularis via scanning and transmission electron microscopy and immunohistochemistry and identify central canal proliferating cells with Ki67 and newly generated cells with bromodeoxyuridine incorporation 3 months after the injection. The central canal is composed of uniciliated, biciliated, and multiciliated ependymal cells, astrocytes, and neurons. Multiciliated ependymal cells show morphological characteristics similar to multiciliated ependymal cells from the lateral ventricles, and uniciliated and biciliated ependymal cells display cilia with large, star-shaped basal bodies, similar to the Ecc cells described for the rodent central canal. Here we show that ependymal cells with one or two cilia, but not multiciliated ependymal cells, proliferate and give rise to new ependymal cells that presumably remain in the macaque central canal. We found that the infant and adult human spinal cord contains ependymal cell types that resemble those present in the macaque. Interestingly, a wide hypocellular layer formed by bundles of intermediate filaments surrounded the central canal both in the monkey and in the human, being more prominent in the stenosed adult human central canal., (Copyright © 2013 Wiley Periodicals, Inc.)
- Published
- 2014
- Full Text
- View/download PDF
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