139 results on '"P. Le Cann"'
Search Results
2. Effect of collagen fibril orientation on the anisotropic properties of peri-implant bone
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Colabella, Lucas, Naili, Salah, Le Cann, Sophie, and Haiat, Guillaume
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- 2024
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3. Epstein-Barr Virus and immune status imprint the immunogenomics of non-Hodgkin lymphomas occurring in immune-suppressed environments
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Marine Baron, Karim Labreche, Marianne Veyri, Nathalie Désiré, Amira Bouzidi, Fatou Seck-Thiam, Frédéric Charlotte, Alice Rousseau, Véronique Morin, Cécilia Nakid-Cordero, Baptiste Abbar, Alberto Picca, Marie Le Cann, Noureddine Balegroune, Nicolas Gauthier, Ioannis Theodorou, Mehdi Touat, Véronique Morel, Franck Bielle, Assia Samri, Agusti Alentorn, Marc Sanson, Damien Roos-Weil, Corinne Haioun, Elsa Poullot, Anne Langlois de Septenville, Frédéric Davi, Amélie Guihot, Pierre-Yves Boelle, Véronique Leblond, Florence Coulet, Jean-Philippe Spano, Sylvain Choquet, Brigitte Autran, and IDeATIon study group
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Non-Hodgkin lymphomas (NHL) commonly occur in immune-deficient (ID) patients, both HIV-infected and transplanted, and are often EBV-driven with cerebral localization, raising the question of tumor immunogenicity, a critical issue for treatment responses. We investigated the immunogenomics of 68 lymphoproliferative disorders from 51 ID (34 posttransplant, 17 HIV+) and 17 immunocompetent patients. Overall, 72% were Large B Cells Lymphoma (LBCL) and 25% were primary central-nervous-system lymphoma (PCNSL) while 40% were EBV-positive. Tumor whole-exome and RNA sequencing, along with a bioinformatics pipeline allowed analysis of tumor mutational burden (TMB), tumor landscape and microenvironment (TME) and prediction of tumor neoepitopes. Both TMB (2.2 vs 3.4/Mb, p=0.001) and neoepitopes numbers (40 vs 200, p=0.00019) were lower in EBVpositive than in EBV-negative NHL, regardless of the immune status. In contrast both EBV and the immune status influenced the tumor mutational profile, with HNRNPF and STAT3 mutations exclusively observed in EBV-positive and ID NHL, respectively. Peripheral blood T-cell responses against tumor neoepitopes were detected in all EBV-negative cases but in only half EBV-positive ones, including responses against IgH-derived MHC-class-II restricted neoepitopes. The TME analysis showed higher CD8 T cell infiltrates in EBVpositive vs EBV-negative NHL, together with a more tolerogenic profile composed of Tregs, type-M2 macrophages and an increased expression of negative immune-regulators. Our results highlight that the immunogenomics of NHL in patients with immunodeficiency primarily relies on the tumor EBV status, while T cell recognition of tumor- and IgH-specific neoepitopes is conserved in EBV-negative patients, offering potential opportunities for future T cell-based immune therapies.
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- 2024
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4. On behalf of the SFGM‐TC: Real‐life use of third‐party virus‐specific T‐cell transfer in immunocompromised transplanted patients
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Esther Hazane Leroyer, Nadine Petitpain, Stéphane Morisset, Bénédicte Neven, Martin Castelle, Sarah Winter, Laetitia Souchet, Véronique Morel, Marie Le Cann, Mony Fahd, Karima Yacouben, Françoise Mechinaud, Marie Ouachée‐Chardin, Cécile Renard, Hélène Labussière Wallet, Marie Angoso, Charlotte Jubert, Patrice Chevallier, Alexandra Léger, Fanny Rialland, Nathalie Dhedin, Christine Robin, Sébastien Maury, Florence Beckerich, David Beauvais, Thomas Cluzeau, Michaël Loschi, Alina Fernster, Marcelo De Carvalho Bittencourt, Maxime Cravat, Karin Bilger, Laurence Clément, Véronique Decot, Mélanie Gauthier, Anne Legendre, Jérôme Larghero, Amani Ouedrani, Guillaume Martin‐Blondel, Cécile Pochon, Loïc Reppel, Hélène Rouard, Stéphanie Nguyen‐Quoc, Jean‐Hugues Dalle, Maud D'Aveni, and Danièle Bensoussan
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2024
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5. High-resolution mass spectrometry identifies delayed biomarkers for improved precision in acetaminophen/paracetamol human biomonitoring
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Eva Gorrochategui, Marc Le Vee, Habiba Selmi, Anne Gérard, Jade Chaker, Annette M. Krais, Christian Lindh, Olivier Fardel, Cécile Chevrier, Pierre Le Cann, Gary W. Miller, Robert Barouki, Bernard Jégou, Thomas Gicquel, David M. Kristensen, and Arthur David
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Human biomonitoring ,High-resolution mass spectrometry ,Acetaminophen/paracetamol ,Exposomics ,Thiomethyl metabolites ,Chemical exposome ,Environmental sciences ,GE1-350 - Abstract
Paracetamol/acetaminophen (N-acetyl-p-aminophenol, APAP) is a top selling analgesic used in more than 600 prescription and non-prescription pharmaceuticals. To study efficiently some of the potential undesirable effects associated with increasing APAP consumption (e.g., developmental disorders, drug-induced liver injury), there is a need to improve current APAP biomonitoring methods that are limited by APAP short half-life. Here, we demonstrate using high-resolution mass spectrometry (HRMS) in several human studies that APAP thiomethyl metabolite conjugates (S-methyl-3-thioacetaminophen sulfate and S-methyl-3-thioacetaminophen sulphoxide sulfate) are stable biomarkers with delayed excretion rates compared to conventional APAP metabolites, that could provide a more reliable history of APAP ingestion in epidemiological studies. We also show that these biomarkers could serve as relevant clinical markers to diagnose APAP acute intoxication in overdosed patients, when free APAP have nearly disappeared from blood. Using in vitro liver models (HepaRG cells and primary human hepatocytes), we then confirm that these thiomethyl metabolites are directly linked to the toxic N-acetyl-p-benzoquinone imine (NAPQI) elimination, and produced via an overlooked pathway called the thiomethyl shunt pathway. Further studies will be needed to determine whether the production of the reactive hepatotoxic NAPQI metabolites is currently underestimated in human. Nevertheless, these biomarkers could already serve to improve APAP human biomonitoring, and investigate, for instance, inter-individual variability in NAPQI production to study underlying causes involved in APAP-induced hepatotoxicity. Overall, our findings demonstrate the potential of exposomics-based HRMS approach to advance towards a better precision for human biomonitoring.
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- 2023
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6. Mechanical micromodeling of stress-shielding at the bone-implant interphase under shear loading
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Hériveaux, Yoann, Le Cann, Sophie, Fraulob, Manon, Vennat, Elsa, Nguyen, Vu-Hieu, and Haïat, Guillaume
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- 2022
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7. Early exposure to farm dust in an allergic airway inflammation rabbit model: Does it affect bronchial and cough hyperresponsiveness?
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Amandine Divaret-Chauveau, Laurent Foucaud, Bruno Demoulin, Cédric Teston, Pauline Loison, Pierre Le Cann, Cyril Schweitzer, Marcelo De Carvalho Bittencourt, Frédéric Mauny, and Silvia Demoulin-Alexikova
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Medicine ,Science - Abstract
IntroductionOver the past 50 years, the prevalence of allergic respiratory diseases has been increasing. The Hygiene hypothesis explains this progression by the decrease in the bio-diversity of early microbial exposure. This study aims to evaluate the effect of early-life farm exposure on airway hyperresponsiveness and cough hypersensitivity in an allergic airway inflammation rabbit model.MethodA specific environment was applied to pregnant rabbits and their offspring until six weeks after birth. Rabbits were housed in a pathogen-free zone for the control group and a calf barn for the farm group. At the end of the specific environmental exposure, both groups were then housed in a conventional zone and then sensitized to ovalbumin. Ten days after sensitization, the rabbit pups received ovalbumin aerosols to provoke airway inflammation. Sensitization to ovalbumin was assessed by specific IgE assay. Cough sensitivity was assessed by mechanical stimulation of the trachea, and bronchial reactivity was assessed by methacholine challenge. The farm environment was characterized by endotoxin measurement.ResultsA total of 38 rabbit pups were included (18 in the farm group). Endotoxin levels in the farm environment varied from 30 to 1854 EU.m-3. There was no significant difference in specific IgE values to ovalbumin (p = 0.826) between the two groups. The mechanical threshold to elicit a cough did not differ between the two groups (p = 0.492). There was no difference in the number of cough (p = 0.270) or the intensity of ventilatory responses (p = 0.735). After adjusting for age and weight, there was no difference in respiratory resistance before and after methacholine challenge.ConclusionEarly exposure to the calf barn did not affect cough sensitivity or bronchial reactivity in ovalbumin-sensitized rabbits. These results suggest that not all farm environments protect against asthma and atopy. Continuous exposure to several sources of microbial diversity is probably needed.
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- 2023
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8. The Hydration State of Bone Tissue Affects Contrast in Neutron Tomographic Images
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Elin Törnquist, Sophie Le Cann, Alessandro Tengattini, Lukas Helfen, Joeri Kok, Stephen A. Hall, and Hanna Isaksson
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neutron tomography ,rat tibiae ,trabecular bovine bone ,image quality ,heavy water ,Biotechnology ,TP248.13-248.65 - Abstract
Neutron tomography has emerged as a promising imaging technique for specific applications in bone research. Neutrons have a strong interaction with hydrogen, which is abundant in biological tissues, and they can penetrate through dense materials such as metallic implants. However, in addition to long imaging times, two factors have led to challenges in running in situ mechanical characterization experiments on bone tissue using neutron tomography: 1) the high water content in specimens reduces the visibility of internal trabecular structures; 2) the mechanical properties of bone are dependent on the hydration state of the tissue, with drying being reported to cause increased stiffness and brittleness. This study investigates the possibility of improving image quality in terms of neutron transmission and contrast between material phases by drying and rehydrating in heavy water. Rat tibiae and trabecular bovine bone plugs were imaged with neutron tomography at different hydration states and mechanical testing of the bone plugs was carried out to assess effects of drying and rehydration on the mechanical properties of bone. From analysis of image histograms, it was found that drying reduced the contrast between bone and soft tissue, but the contrast was restored with rehydration. Contrast-to-noise ratios and line profiles revealed that the contrast between bone tissue and background was reduced with increasing rehydration duration but remained sufficient for identifying internal structures as long as no free liquid was present inside the specimen. The mechanical analysis indicated that the proposed fluid exchange protocol had no adverse effects on the mechanical properties.
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- 2022
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9. The difficulty to model Huntington’s disease in vitro using striatal medium spiny neurons differentiated from human induced pluripotent stem cells
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Kim Le Cann, Alec Foerster, Corinna Rösseler, Andelain Erickson, Petra Hautvast, Sebastian Giesselmann, Daniel Pensold, Ingo Kurth, Markus Rothermel, Virginia B. Mattis, Geraldine Zimmer-Bensch, Stephan von Hörsten, Bernd Denecke, Tim Clarner, Jannis Meents, and Angelika Lampert
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Medicine ,Science - Abstract
Abstract Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder caused by an expanded polyglutamine repeat in the huntingtin gene. The neuropathology of HD is characterized by the decline of a specific neuronal population within the brain, the striatal medium spiny neurons (MSNs). The origins of this extreme vulnerability remain unknown. Human induced pluripotent stem cell (hiPS cell)-derived MSNs represent a powerful tool to study this genetic disease. However, the differentiation protocols published so far show a high heterogeneity of neuronal populations in vitro. Here, we compared two previously published protocols to obtain hiPS cell-derived striatal neurons from both healthy donors and HD patients. Patch-clamp experiments, immunostaining and RT-qPCR were performed to characterize the neurons in culture. While the neurons were mature enough to fire action potentials, a majority failed to express markers typical for MSNs. Voltage-clamp experiments on voltage-gated sodium (Nav) channels revealed a large variability between the two differentiation protocols. Action potential analysis did not reveal changes induced by the HD mutation. This study attempts to demonstrate the current challenges in reproducing data of previously published differentiation protocols and in generating hiPS cell-derived striatal MSNs to model a genetic neurodegenerative disorder in vitro.
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- 2021
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10. Quantitative ultrasound assessment of the influence of roughness and healing time on osseointegration phenomena
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M. Fraulob, R. Vayron, S. Le Cann, B. Lecuelle, Y. Hériveaux, H. Albini Lomami, C. H. Flouzat Lachaniette, and G. Haïat
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Medicine ,Science - Abstract
Abstract The evolution of bone tissue quantity and quality in contact with the surface of orthopedic and dental implants is a strong determinant of the surgical outcome but remains difficult to be assessed quantitatively. The aim of this study was to investigate the performance of a quantitative ultrasound (QUS) method to measure bone-implant interface (BII) properties. A dedicated animal model considering coin-shaped titanium implants with two levels of surface roughness (smooth, S a = 0.49 µm and rough, S a = 3.5 µm) allowed to work with a reproducible geometry and a planar interface. The implants were inserted in rabbit femurs and tibiae for 7 or 13 weeks. The ultrasonic response of the BII was measured ex vivo, leading to the determination of the 2-D spatial variations of bone in contact with the implant surface. Histological analysis was carried out to determine the bone-implant contact (BIC) ratio. The amplitude of the echo was significantly higher after 7 weeks of healing time compared to 13 weeks, for both smooth (p
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- 2020
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11. The difficulty to model Huntington’s disease in vitro using striatal medium spiny neurons differentiated from human induced pluripotent stem cells
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Le Cann, Kim, Foerster, Alec, Rösseler, Corinna, Erickson, Andelain, Hautvast, Petra, Giesselmann, Sebastian, Pensold, Daniel, Kurth, Ingo, Rothermel, Markus, Mattis, Virginia B., Zimmer-Bensch, Geraldine, von Hörsten, Stephan, Denecke, Bernd, Clarner, Tim, Meents, Jannis, and Lampert, Angelika
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- 2021
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12. Asthma and Indoor Environment: Usefulness of a Global Allergen Avoidance Method on Asthma Control and Exposure to Molds
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Gangneux, Jean-Pierre, Bouvrais, Matthieu, Frain, Sophie, Morel, Hugues, Deguen, Séverine, Chevrier, Sylviane, and Le Cann, Pierre
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- 2020
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13. Analysis of Particulate and Microbiological Filtration Performance of Air Handling Unit Filters in a Low-Energy Office Building over 12 Months
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Gaëtan Pavard, Aurélie Joubert, Yves Andrès, and Pierre Le Cann
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indoor air quality ,air handling units ,filters ,filtration efficiency ,particle ,bacteria ,Building construction ,TH1-9745 - Abstract
Indoor air quality is an important consideration for the health and well-being of building occupants, and the SARS CoV-2 pandemic highlighted the importance of maintaining proper ventilation in buildings. Air handling units (AHUs) are used to provide fresh air and maintain occupant comfort. The objective of this work was to study the evolution of filtration efficiency in an AHU fitted with bag filters, installed to treat office air in a low-energy building, over a 12-month period. The particulate filtration efficiency (PFE) and the microbial filtration efficiency (MFE) were quantified by measuring particle size distribution and bacterial and fungal concentration in the air circulating in the AHU. The resulting microbial concentration measurements in the fresh air (between 10²–103 CFU/m3 for fungi and around 103 CFU/m3 for bacteria) were higher than those in the extracted air from the offices (between 101 and 102 CFU/m3 for fungi and around 102 CFU/m3 for bacteria). The PFE and MFE measured were almost constant throughout the 12 months, with an increase of the filter pressure drop from 70 to 90 Pa. The PFE and MFE were quite comparable for a particle diameter. Therefore, the measurement of PFE is a reliable indicator of the MFE.
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- 2022
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14. Multiscale Characterization of Embryonic Long Bone Mineralization in Mice
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Isabella Silva Barreto, Sophie Le Cann, Saima Ahmed, Vivien Sotiriou, Mikael J. Turunen, Ulf Johansson, Angel Rodriguez‐Fernandez, Tilman A. Grünewald, Marianne Liebi, Niamh C. Nowlan, and Hanna Isaksson
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bone development ,Fourier transform infra‐red microspectroscopy ,small‐ and wide‐angle X‐ray scattering ,X‐ray fluorescence spectroscopy ,X‐ray tomography ,Science - Abstract
Abstract Long bone mineralization occurs through endochondral ossification, where a cartilage template mineralizes into bone‐like tissue with a hierarchical organization from the whole bone‐scale down to sub‐nano scale. Whereas this process has been extensively studied at the larger length scales, it remains unexplored at some of the smaller length scales. In this study, the changes in morphology, composition, and structure during embryonic mineralization of murine humeri are investigated using a range of high‐resolution synchrotron‐based imaging techniques at several length scales. With micro‐ and nanometer spatial resolution, the deposition of elements and the shaping of mineral platelets are followed. Rapid mineralization of the humeri occurs over approximately four days, where mineral to matrix ratio and calcium content in the most mineralized zone reach adult values shortly before birth. Interestingly, zinc is consistently found to be localized at the sites of ongoing new mineralization. The mineral platelets in the most recently mineralized regions are thicker, longer, narrower, and less aligned compared to those further into the mineralized region. In summary, this study demonstrates a specific spatial distribution of zinc, with highest concentration where new mineral is being deposited and that the newly formed mineral platelets undergo slight reshaping and reorganization during embryonic development.
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- 2020
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15. Indoor Carbon Dioxide, Fine Particulate Matter and Total Volatile Organic Compounds in Private Healthcare and Elderly Care Facilities
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Alexandre Baudet, Estelle Baurès, Olivier Blanchard, Pierre Le Cann, Jean-Pierre Gangneux, and Arnaud Florentin
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indoor air quality ,carbon dioxide ,particulate matter ,total volatile organic compounds ,dental offices ,general practitioner offices ,Chemical technology ,TP1-1185 - Abstract
Poor indoor air quality can have adverse effects on human health, especially in susceptible populations. The aim of this study was to measure the concentrations of dioxide carbon (CO2), fine particulate matter (PM2.5) and total volatile organic compounds (TVOCs) in situ in private healthcare and elderly care facilities. These pollutants were continuously measured in two rooms of six private healthcare facilities (general practitioner’s offices, dental offices and pharmacies) and four elderly care facilities (nursing homes) in two French urban areas during two seasons: summer and winter. The mean CO2 concentrations ranged from 764 ± 443 ppm in dental offices to 624 ± 198 ppm in elderly care facilities. The mean PM2.5 concentrations ranged from 13.4 ± 14.4 µg/m3 in dental offices to 5.7 ± 4.8 µg/m3 in general practitioner offices. The mean TVOC concentrations ranged from 700 ± 641 ppb in dental offices to 143 ± 239 ppb in general practitioner offices. Dental offices presented higher levels of indoor air pollutants, associated with the dental activities. Increasing the ventilation of these facilities by opening a window is probably an appropriate method for reducing pollutant concentrations and maintaining good indoor air quality.
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- 2022
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16. Quantitative Health Risk Assessment of the Chronic Inhalation of Chemical Compounds in Healthcare and Elderly Care Facilities
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Anaïs Colas, Alexandre Baudet, Pierre Le Cann, Olivier Blanchard, Jean-Pierre Gangneux, Estelle Baurès, and Arnaud Florentin
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health risk assessment ,indoor air pollution ,chronic inhalation ,chemical compounds ,healthcare facility ,hospital ,Chemical technology ,TP1-1185 - Abstract
Previous studies have described the chemical pollution in indoor air of healthcare and care facilities. From these studies, the main objective of this work was to conduct a quantitative health risk assessment of the chronic inhalation of chemical compounds by workers in healthcare and elderly care facilities (hospitals, dental and general practitioner offices, pharmacies and nursing homes). The molecules of interest were 36 volatile and 13 semi-volatile organic compounds. Several professional exposure scenarios were developed in these facilities. The likelihood and severity of side effects that could occur were assessed by calculating the hazard quotient for deterministic effects, and the excess lifetime cancer risk for stochastic effects. No hazard quotient was greater than 1. Three compounds had a hazard quotient above 0.1: 2-ethyl-1-hexanol in dental and general practitioner offices, ethylbenzene and acetone in dental offices. Only formaldehyde presented an excess lifetime cancer risk greater than 1 × 10−5 in dental and general practitioner offices (maximum value of 3.8 × 10−5 for general practitioners). The health risk for chronic inhalation of most compounds investigated did not appear to be of concern. Some values tend to approach the acceptability thresholds justifying a reflection on the implementation of corrective actions such as the installation of ventilation systems.
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- 2022
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17. Iron deficiency, an unusual cause of thrombocytopenia: results from a multicenter retrospective case-controlled study
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Huscenot, Tessa, Darnige, Luc, Wagner-Ballon, Orianne, Ronchetti, Anne-Marie, Lousteau, Valentine, Limal, Nicolas, Morbieu, Caroline, Gobert, Delphine, Rohmer, Julien, Mathian, Alexis, Le Cann, Marie, Fadlallah, Jehane, Languille, Laetitia, and Michel, Marc
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- 2019
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18. Quantitative ultrasound assessment of the influence of roughness and healing time on osseointegration phenomena
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Fraulob, M., Vayron, R., Le Cann, S., Lecuelle, B., Hériveaux, Y., Albini Lomami, H., Flouzat Lachaniette, C. H., and Haïat, G.
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- 2020
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19. Bone Damage Evolution Around Integrated Metal Screws Using X-Ray Tomography — in situ Pullout and Digital Volume Correlation
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Sophie Le Cann, Erika Tudisco, Magnus Tägil, Stephen A. Hall, and Hanna Isaksson
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X-ray tomography ,bone ,metallic screw ,in situ loading ,Digital Volume Correlation ,Biotechnology ,TP248.13-248.65 - Abstract
Better understanding of the local deformation of the bone network around metallic implants subjected to loading is of importance to assess the mechanical resistance of the bone-implant interface and limit implant failure. In this study, four titanium screws were osseointegrated into rat tibiae for 4 weeks and screw pullout was conducted in situ under x-ray microtomography, recording macroscopic mechanical behavior and full tomographies at multiple load steps before failure. Images were analyzed using Digital Volume Correlation (DVC) to access internal displacement and deformation fields during loading. A repeatable failure pattern was observed, where a ∼300–500 μm-thick envelope of bone detached from the trabecular structure. Fracture initiated close to the screw tip and propagated along the implant surface, at a distance of around 500 μm. Thus, the fracture pattern appeared to be influenced by the microstructure of the bone formed closely around the threads, which confirmed that the model is relevant for evaluating the effect of pharmacological treatments affecting local bone formation. Moreover, cracks at the tibial plateau were identified by DVC analysis of the tomographic images acquired during loading. Moderate strains were first distributed in the trabecular bone, which localized into higher strains regions with subsequent loading, revealing crack-formation not evident in the tomographic images. The in situ loading methodology followed by DVC is shown to be a powerful tool to study internal deformation and fracture behavior of the newly formed bone close to an implant when subjected to loading. A better understanding of the interface failure may help improve the outcome of surgical implants.
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- 2020
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20. Metagenomic Characterization of Indoor Dust Bacterial and Fungal Microbiota in Homes of Asthma and Non-asthma Patients Using Next Generation Sequencing
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Jean-Pierre Gangneux, Mohamed Sassi, Pierre Lemire, and Pierre Le Cann
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microbiota ,mycobiota ,asthma ,indoor environment ,dust ,next-generation sequencing ,Microbiology ,QR1-502 - Abstract
BackgroundThe exposure of house occupants to indoor air pollutants has increased in recent decades. Among microbiological contaminants, bacterial and fungal aerosols remain poorly studied and the debate on the impact of these aerosols on respiratory health is still open. This study aimed to assess the diversity of indoor microbial communities in relationship with the health of occupants.MethodsMeasurements were taken from dwellings of 2 cohorts in Brittany (France), one with children without any pathology and the other with children and adults with asthma. Thirty dust samples were analyzed by next generation sequencing with a 16S and 18S targeted metagenomics approach. Analysis of sequencing data was performed using qiime 2, and univariate and multivariate statistical analysis using R software and phyloseq package.ResultsA total of 2,637 prokaryotic (589 at genus level) and 2,153 eukaryotic taxa were identified (856 fungal taxa (39%) and 573 metazoa (26%)). The four main bacterial phyla were identified: Proteobacteria (53%), Firmicutes (27%), Actinobacteria (11%), Bacteroidetes (8%). Among Fungi, only 136 taxa were identified at genus level. Three main fungal phyla were identified: Ascomycota (84%), Basidiomycota (12%) and Mucoromycota (3%). No bacterial nor fungal phyla were significantly associated with asthma versus control group. A significant over representation in control group versus asthma was observed for Christensenellaceae family (p-value = 0.0015, adj. p-value = 0.033). Besides, a trend for over representation in control group was observed with Dermabacteraceae family (p-value = 0.0002, adj. p-value = 0.815).ConclusionsOur findings provide evidence that dust samples harbor a high diversity of human-associated bacteria and fungi. Molecular methods such as next generation sequencing are reliable tools for identifying and tracking the bacterial and fungal diversity in dust samples, a less easy strategy for the detection of eukaryotes at least using18S metagenomics approach. This study showed that the detection of some bacteria might be associated to indoor air of asthmatic patients. Regarding fungi, a higher number of samples and sequencing with more depth could allow reaching significant signatures.
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- 2020
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21. Sub-trabecular strain evolution in human trabecular bone
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Turunen, Mikael J., Le Cann, Sophie, Tudisco, Erika, Lovric, Goran, Patera, Alessandra, Hall, Stephen A., and Isaksson, Hanna
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- 2020
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22. Indoor Air Quality in Healthcare and Care Facilities: Chemical Pollutants and Microbiological Contaminants
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Alexandre Baudet, Estelle Baurès, Hélène Guegan, Olivier Blanchard, Monique Guillaso, Pierre Le Cann, Jean-Pierre Gangneux, and Arnaud Florentin
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indoor air quality ,organic compounds ,particulate matter ,environmental microbiology ,environmental pollutants ,health facility environment ,Meteorology. Climatology ,QC851-999 - Abstract
The indoor air quality of healthcare and care facilities is poorly studied. The aim of this study was to qualitatively and quantitatively describe the chemical pollution and the microbiological contaminations of the indoor environment of these facilities. Methods: A wide range of chemical compounds (39 volatile and 13 semi-volatile organic compounds, carbon dioxide, fine particulate matter) and microorganisms (fungi and bacteria) were studied. Sampling campaigns were conducted in two French cities in summer 2018 and winter 2019 in six private healthcare facilities (general practitioner’s offices, dental offices, pharmacies) and four care facilities (nursing homes). Results: The highest median concentrations of chemical compounds (μg/m3) were measured for alcohols (ethanol: 378.9 and isopropanol: 23.6), ketones (acetone: 18.8), aldehydes (formaldehyde: 11.4 and acetaldehyde: 6.5) and terpenes (limonene: 4.3). The median concentration of PM2.5 was 9.0 µg/m3. The main bacteria of these indoor environments were Staphylococcus, Micrococcus and Bacillus genera, with median bacterial concentrations in the indoor air of 14 cfu/m3. The two major fungal genera were Cladosporium and Penicillium, with median fungal concentrations of 7 cfu/m3. Conclusions: Indoor air in healthcare and care facilities contains a complex mixture of many pollutants found in higher concentrations compared to the indoor air in French hospitals in a previous study.
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- 2021
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23. The Fate of Mengovirus on Fiberglass Filter of Air Handling Units
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Bandaly, Victor, Joubert, Aurélie, Le Cann, Pierre, and Andres, Yves
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- 2017
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24. Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic Imaging
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Sophie Le Cann, Erika Tudisco, Mikael J. Turunen, Alessandra Patera, Rajmund Mokso, Magnus Tägil, Ola Belfrage, Stephen A. Hall, and Hanna Isaksson
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X-ray tomography ,bone ,metallic screw ,in situ loading ,synchrotron ,Biotechnology ,TP248.13-248.65 - Abstract
Long-term stability of endosseous implants depends on successful bone formation, ingrowth and adaptation to the implant. Specifically, it will define the mechanical properties of the newly formed bone-implant interface. 3D imaging during mechanical loading tests (in situ loading) can improve the understanding of the local processes leading to bone damage and failure. In this study, titanium screws were implanted into rat tibiae and were allowed to integrate for 4 weeks with or without the addition of the growth factor Bone Morphogenetic Protein and the bisphosphonate Zoledronic Acid. Samples were subjected to in situ pullout using high-resolution synchrotron x-ray tomography at the Tomcat beamline (SLS, PSI, Switzerland) at 30 keV with 25 ms exposure time, resulting in a total acquisition time of 45 s per scan, with a 3.6 μm isotropic voxel size. Using a custom-made loading device positioned inside the beamline, screws were pulled out with 0.05 mm increment, acquiring multiple scans until rupture of the sample. The in situ loading protocol was adapted to ensure short imaging time, which enabled multiple samples to be tested with short loading steps, while keeping the total testing time low and reducing dose deposition. Higher trabecular bone content was quantified in the surrounding of the screw in the treated groups, which correlated with increased mechanical strength and stiffness. Differences in screw implantation, such as contact between threads and cortex as well as minor tilt of the screw were also correlated to the mechanical parameters. In situ loading enabled the investigation of crack propagation during the pullout, highlighting the mechanical behavior of the interface. Three typical crack types were observed: (1) rupture at the interface of trabecular and cortical bone tissues, close to the screw, (2) large crack inside the cortex connected to the implant, and (3) first failure away from the screw with cracks propagating toward the screw-bone interface. Mechanical properties of in vivo integrated bone-metal screws rely on a combination of multiple parameters that are difficult to identify and separate one from the other.
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- 2019
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25. Large diurnal bottom temperature oscillations around the Saint Pierre and Miquelon archipelago
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Lazure, Pascal, Le Cann, Bernard, and Bezaud, Marion
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- 2018
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26. A yeast-based assay identifies drugs that interfere with immune evasion of the Epstein-Barr virus
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Cécile Voisset, Chrysoula Daskalogianni, Marie-Astrid Contesse, Anne Mazars, Hratch Arbach, Marie Le Cann, Flavie Soubigou, Sébastien Apcher, Robin Fåhraeus, and Marc Blondel
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EBV-associated cancers ,Cell-based drug screening ,EBNA1 GAr domain ,Yeast-based models ,Immune evasion ,Doxorubicin ,Daunorubicin ,5-fluorouracil ,Medicine ,Pathology ,RB1-214 - Abstract
Epstein-Barr virus (EBV) is tightly associated with certain human cancers, but there is as yet no specific treatment against EBV-related diseases. The EBV-encoded EBNA1 protein is essential to maintain viral episomes and for viral persistence. As such, EBNA1 is expressed in all EBV-infected cells, and is highly antigenic. All infected individuals, including individuals with cancer, have CD8+ T cells directed towards EBNA1 epitopes, yet the immune system fails to detect and destroy cells harboring the virus. EBV immune evasion depends on the capacity of the Gly-Ala repeat (GAr) domain of EBNA1 to inhibit the translation of its own mRNA in cis, thereby limiting the production of EBNA1-derived antigenic peptides presented by the major histocompatibility complex (MHC) class I pathway. Here we establish a yeast-based assay for monitoring GAr-dependent inhibition of translation. Using this assay we identify doxorubicin (DXR) as a compound that specifically interferes with the GAr effect on translation in yeast. DXR targets the topoisomerase-II–DNA complexes and thereby causes genomic damage. We show, however, that the genotoxic effect of DXR and various analogs thereof is uncoupled from the effect on GAr-mediated translation control. This is further supported by the observation that etoposide and teniposide, representing another class of topoisomerase-II–DNA targeting drugs, have no effect on GAr-mediated translation control. DXR and active analogs stimulate, in a GAr-dependent manner, EBNA1 expression in mammalian cells and overcome GAr-dependent restriction of MHC class I antigen presentation. These results validate our approach as an effective high-throughput screening assay to identify drugs that interfere with EBV immune evasion and, thus, constitute candidates for treating EBV-related diseases, in particular EBV-associated cancers.
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- 2014
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27. Évaluation multicentrique et randomisée de l’impact des conseillers en environnement intérieur sur le contrôle de l’asthme : l’étude ECENVIR
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J.P. Gangneux, H. Morel, F.X. Blanc, P. Bonniaud, G. Boza, A. Briault, J. Brouard, D. Charpin, C. Chenivesse, H. Colas, A. Cottereau, G. Dalampira, F. De Blay, X. Debeillex, P. Dumont, K. Elchehaded, M. Fayon, S. Fry, E. Hullo, A. Leblanc, J. Letreut, A. Magnan, G. Mahay, J.M. Perotin-Collard, H. Pegliasco, I. Pin, C. Pison, N. Rossignoli, V. Siao Him Fa, H. Ziani Bey, L. Com-Ruelle, J. Morcet, E. Oger, P. Le Cann, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), Laboratoire d'étude et de recherche en environnement et santé (LERES), Département des sciences en santé environnementale (DEESSE), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Département Santé Environnement Travail et Génie Sanitaire (DSETGS)
- Subjects
Visite ,Immunology and Allergy ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Asthme ,Etude ECENVIR ,Conseillers en environnement intérieur (CEI) - Abstract
International audience; Introduction (contexte de la recherche) :L’exposition répétée des patients asthmatiques aux allergènes intradomiciliaires contribue aux exacerbations de l’asthme. Plusieurs stratégies d’intervention sont proposées, parmi lesquelles, la visite domiciliaire de conseillers en environnement intérieur (CEI). Les CEI établissent un état des lieu au domicile du patient et proposent des mesures de remédiation afin d’améliorer les fonctions respiratoires. Plusieurs études monocentriques ont montré l’intérêt d’une telle démarche.ObjectifL’étude ECENVIR est un essai clinique, multicentrique, prospectif et randomisé qui vise à évaluer l’impact des CEI sur le niveau de contrôle de l’asthme.Méthodes :Au total, 104 patients ont été inclus dont 85 patients ont été suivis médicalement et randomisés dans 2 groupes : le Groupe Intervention (GI) a bénéficié d’une visite de CEI avec prélèvements pour mesures d’allergènes et conseils à l’inclusion et après 12 mois de suivi ; et le groupe témoin (GT) a bénéficié d’une même visite de contrôle uniquement à M12.Résultats :Le critère de jugement principal a été analysé chez 71/85 patients : 34 dans le GI et 37 dans le GT. A M12, une amélioration du contrôle de l’asthme était notée dans le GI avec une diminution du nombre d’asthme sévère et une augmentation d’asthme intermittent (p = 0,05) comparativement au GT (p = 0,24). En comparaison intergroupe, 50 % (17/34) des patients du GI ont montré une amélioration clinique comparativement au GT (p = 0,43). Bien que non statistiquement significative probablement dû à un manque de puissance, les patients du GI avaient 1,5 fois plus de chance de s’améliorer cliniquement en analyse multivariée (OR 1,47 CI95 % [0,57–3,75]. Les analyses secondaires montrent que : (i) dans la population des asthmes sévères, 12/16 patients (75 %) du GI se sont améliorés contre 7/16 du GT(44 %)(p = 0,06) ; (ii) la consommation des corticoïdes oraux et le taux d’hospitalisation sont significativement diminués dans le groupe ayant bénéficié de conseils (p = 0,029 et p = 0,042, respectivement).Conclusions :L’intervention des CEI est bénéfique cliniquement sur le niveau du contrôle de l’asthme et permet sur le plan économique une diminution de la consommation de soins, en cours d’investigation via les données d’Assurance maladie.
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- 2022
28. POINT SUR LES PROJETS DE NORMALISATION « AIR DES LIEUX DE TRAVAIL » RELATIFS AUX AGENTS BIOLOGIQUES AUX NIVEAUX EUROPÉEN ET FRANÇAIS
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P. DUQUENNE, P. LE CANN, B. FACON, O. SCHLOSSER, E. DALIBERT, M. DUSSÉAUX, A. SACHOT, P. ARNOULD, P. LOISON, L. DUPONT, I. CHEVALIER-ALLIO, and E. BARZYKOWSKI
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Biological agents ,Prévention ,Measurement methods and strategies ,Prevention ,Méthodes et stratégies de mesure ,Working in network ,Agents biologiques ,travail en réseau - Abstract
La présentation fait un état des lieux des travaux de normalisation menés aux niveaux européen et français sur la période 2018-2021 et précise les orientations prévues après 2022. Les normes EN 13098, EN 14031 et EN 14583 sur les agents biologiques ont été révisées par le groupe de travail CEN/TC 137/WG5 « Mesurage des agents biologiques ». Ce même groupe propose des projets de normes concernant les mesures des mycotoxines, des virus dans l'air et des agents biologiques par les techniques de biologie moléculaire. Il propose aussi d'accentuer la promotion de la recherche pour accompagner la normalisation et le travail en réseau. Au niveau français, le groupe miroir AFNOR X43C/GE1 suit ces travaux et a engagé un travail sur la stratégie de mesure. Les travaux de normalisation ont créé une dynamique nouvelle et fédératrice sur le thème des risques biologiques et permettent de diffuser la culture et la démarche de prévention., The presentation provides an overview of the standardisation work carried out at European and French level over the 2018-2021 period and specifies the directions that have been given after 2022. The standards EN 13098, EN 14031 and EN 14583 relative to biological agents have been revised by the working group CEN/TC 137/WG5 "Measurement of biological agents". The same group is proposing draft standards for the measurement of mycotoxins, viruses in air and biological agents by molecular techniques. It also proposes to increase the promotion of research to support standardisation and networking. At the French level, the AFNOR X43C/GE1 mirror group is following this work and has initiated work on the measurement strategy. The standardisation work has created a new and unifying dynamic on the issue of biological risks and has made it possible to disseminate the culture and the prevention approach.
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- 2022
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29. EVALUATION D'UNE MÉTHODOLOGIE UTILISANT LES FILTRES DE REPRISE DES CENTRALES DE TRAITEMENT D'AIR POUR QUALIFIER LA QUALITÉ MICROBIOLOGIQUE DE L'AIR INTÉRIEUR DES BUREAUX
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G. PAVARD, A. JOUBERT, Y. ANDRÈS, and P. LE CANN
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Qualité microbiologique de l'air ,Microbiological air quality ,Air Handling Units ,Centrale de Traitement d'Air ,Offices ,Filtres ,Filters ,Aérosols microbiens ,Microbial aerosols ,Bureaux - Abstract
La pandémie Covid-19 a permis de rappeler l'importance de maintenir une qualité de l'air intérieur convenable pour diminuer les risques de propagation des particules virales. L'objectif de cette étude est de tester une méthodologie utilisant les filtres de reprise des centrales de traitement d'air pour identifier les contaminants microbiens présents dans l'air intérieur de bureaux. La méthodologie met en oeuvre des coupons de média filtrant prélevés périodiquement sur les filtres et analysés par méthodes culturale et moléculaire. Les résultats montrent que la concentration en microorganismes cultivables sur les filtres se stabilise après 4-5 mois et les genres Aspergillus, Cladosporium et Penicillium sont majoritaires., The Covid-19 pandemic reminded us of the importance of maintaining adequate indoor air quality to reduce the risk of propagation of viral particles. The aim of this study is to use air handling unit (AHU) filters to develop a methodology to identify microbial contaminants present in office indoor air. The methodology involves discs of filter media taken periodically from the extraction filters and analysed by cultural and molecular methods. The concentration of cultivable mircoorganisms on the filters stabilises after 4-5 months. The genera Aspergillus, Cladosporium and Penicillium are the most represented.
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- 2022
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30. Pedicle Screw Fixation Study in Immature Porcine Spines to Improve Pullout Resistance during Animal Testing.
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Sophie Le Cann, Thibaut Cachon, Eric Viguier, Lotfi Miladi, Thierry Odent, Jean-Marie Rossi, and Patrick Chabrand
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Medicine ,Science - Abstract
The porcine model is frequently used during development and validation of new spinal devices, because of its likeness to the human spine. These spinal devices are frequently composed of pedicle screws with a reputation for stable fixation but which can suffer pullouts during preclinical implantation on young animals, leading to high morbidity. With a view to identifying the best choices to optimize pedicle screw fixation in the porcine model, this study evaluates ex vivo the impact of weight (age) of the animal, the level of the vertebrae (lumbar or thoracic) and the type of screw anchorage (mono- or bi-cortical) on pedicle screw pullouts. Among the 80 pig vertebrae (90- and 140-day-old) tested in this study, the average screw pullout forces ranged between 419.9N and 1341.2N. In addition, statistical differences were found between test groups, pointing out the influence of the three parameters stated above. We found that the the more caudally the screws are positioned (lumbar level), the greater their pullout resistance is, moreover, screw stability increases with the age, and finally, the screws implanted with a mono-cortical anchorage sustained lower pullout forces than those implanted with a bi-cortical anchorage. We conclude that the best anchorage can be obtained with older animals, using a lumbar fixation and long screws traversing the vertebra and inducing bi-cortical anchorage. In very young animals, pedicle screw fixations need to be bi-cortical and more numerous to prevent pullout.
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- 2015
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31. The Disintegrin and Metalloprotease ADAM12 Is Associated with TGF-β-Induced Epithelial to Mesenchymal Transition.
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Michaël Ruff, Anthony Leyme, Fabienne Le Cann, Dominique Bonnier, Jacques Le Seyec, Franck Chesnel, Laurent Fattet, Ruth Rimokh, Georges Baffet, and Nathalie Théret
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Medicine ,Science - Abstract
The increased expression of the Disintegrin and Metalloprotease ADAM12 has been associated with human cancers, however its role remain unclear. We have previously reported that ADAM12 expression is induced by the transforming growth factor, TGF-β and promotes TGF-β-dependent signaling through interaction with the type II receptor of TGF-β. Here we explore the implication of ADAM12 in TGF-β-mediated epithelial to mesenchymal transition (EMT), a key process in cancer progression. We show that ADAM12 expression is correlated with EMT markers in human breast cancer cell lines and biopsies. Using a non-malignant breast epithelial cell line (MCF10A), we demonstrate that TGF-β-induced EMT increases expression of the membrane-anchored ADAM12L long form. Importantly, ADAM12L overexpression in MCF10A is sufficient to induce loss of cell-cell contact, reorganization of actin cytoskeleton, up-regulation of EMT markers and chemoresistance. These effects are independent of the proteolytic activity but require the cytoplasmic tail and are specific of ADAM12L since overexpression of ADAM12S failed to induce similar changes. We further demonstrate that ADAM12L-dependent EMT is associated with increased phosphorylation of Smad3, Akt and ERK proteins. Conversely, inhibition of TGF-β receptors or ERK activities reverses ADAM12L-induced mesenchymal phenotype. Together our data demonstrate that ADAM12L is associated with EMT and contributes to TGF-β-dependent EMT by favoring both Smad-dependent and Smad-independent pathways.
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- 2015
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32. Lagrangian circulation of the North Atlantic Central Water over the abyssal plain and continental slopes of the Bay of Biscay: description of selected mesoscale features
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Alain Serpette, Bernard Le Cann, and François Colas
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bay of biscay ,lagrangian floats and surface drifters ,slope current ,mesoscale eddies ,slope ocean exchanges ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Between 1994 and 2001, several experiments (ARCANE, SEFOS, INTERAFOS) were conducted to directly measure the general and mesoscale Lagrangian circulations over the Bay of Biscay abyssal plain and slopes. Two levels (~100 m and ~450 m) were selected to cover the North Atlantic Central Water range. Two types of Lagrangian instruments, drogued surface drifters tracked by satellite (Surdrift) and acoustically tracked subsurface floats (Rafos and Marvor), were used. Overall, more than 36 instrument-years were collected in the Bay of Biscay region (43-49°N, 01-12°W). The weak general circulation in the Bay of Biscay is seen to be highly influenced by the occurrence of several mesoscale coherent features, notably slope currents and eddies, and these affect the exchanges between the abyssal plain and the slopes. The objective of this paper is to depict some specific examples of the observed mesoscale field. Selected float trajectories are shown and used to discuss observations of slope currents and of both anticyclonic and cyclonic eddies. Slope currents exhibit alternation of poleward and equatorward directions, depending on both the period and the geographic area considered. Although the generation process of mesoscale eddies is difficult to observe unambiguously from Lagrangian instruments, eddies are nevertheless ubiquitous over the abyssal plain. Some characteristics of the observed cyclonic and anticyclonic eddies are presented. Smaller anticyclones, localised over the outer shelf and interpreted in terms of ajustment of slope water intrusions, are also depicted.
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- 2006
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33. Pullout strength of all suture anchors in the repair of rotator cuff tears: a biomechanical study
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Galland, Alexandre, Airaudi, Stéphane, Gravier, Renaud, Le Cann, Sophie, Chabrand, Patrick, and Argenson, Jean-Noël
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- 2013
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34. A high-resolution modeling study of the Western Iberian Margin mean and seasonal upper ocean circulation
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Nolasco, Rita, Pires, Ana Cordeiro, Cordeiro, Nuno, Le Cann, Bernard, and Dubert, Jesus
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- 2013
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35. Factors associated with post-seasonal serological titer and risk factors for infection with the pandemic A/H1N1 virus in the French general population.
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Nathanael Lapidus, Xavier de Lamballerie, Nicolas Salez, Michel Setbon, Rosemary M Delabre, Pascal Ferrari, Nanikaly Moyen, Marie-Lise Gougeon, Frédéric Vely, Marianne Leruez-Ville, Laurent Andreoletti, Simon Cauchemez, Pierre-Yves Boëlle, Eric Vivier, Laurent Abel, Michaël Schwarzinger, Michèle Legeas, Pierre Le Cann, Antoine Flahault, and Fabrice Carrat
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Medicine ,Science - Abstract
The CoPanFlu-France cohort of households was set up in 2009 to study the risk factors for infection by the pandemic influenza virus (H1N1pdm) in the French general population. The authors developed an integrative data-driven approach to identify individual, collective and environmental factors associated with the post-seasonal serological H1N1pdm geometric mean titer, and derived a nested case-control analysis to identify risk factors for infection during the first season. This analysis included 1377 subjects (601 households). The GMT for the general population was 47.1 (95% confidence interval (CI): 45.1, 49.2). According to a multivariable analysis, pandemic vaccination, seasonal vaccination in 2009, recent history of influenza-like illness, asthma, chronic obstructive pulmonary disease, social contacts at school and use of public transports by the local population were associated with a higher GMT, whereas history of smoking was associated with a lower GMT. Additionally, young age at inclusion and risk perception of exposure to the virus at work were identified as possible risk factors, whereas presence of an air humidifier in the living room was a possible protective factor. These findings will be interpreted in light of the longitudinal analyses of this ongoing cohort.
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- 2013
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36. QUALITÉ DE L'AIR INTÉRIEUR EN STRUCTURES MÉDICO-SOCIALES ET LIBÉRALES : CONTAMINATION CHIMIQUE ET MICROBIOLOGIQUE
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A. BAUDET, E. BAURÈS, O. BLANCHARD, J.P. GANGNEUX, H. GUEGAN, M. GUILLASO, E. SURGET, P. LE CANN, and A. FLORENTIN
- Subjects
Microbiologie environnementale ,Environmental microbiology ,Organic compounds ,Composés organiques ,Indoor air quality ,Particulate matter ,Matière particulaire ,Qualité de l'air intérieur - Abstract
La qualité de l'air des structures médico-sociales et libérales ne bénéficie que d'une exploration très limitée. Le but de notre étude est de décrire qualitativement et quantitativement la contamination microbiologique, chimique et particulaire de l'environnement intérieur de ces structures. Des campagnes de mesures ont été réalisées en été 2018 et hiver 2019 sur Rennes et Nancy dans six structures de soins libérales et quatre structures médico-sociales pour séniors. Les premiers résultats montrent que l'air intérieur comporte un mélange complexe de polluants retrouvés en concentrations assez faibles, en deçà des valeurs guides de l'air intérieur., The indoor air quality of the medico-social and liberal facilities is poorly study. The aim of our study is to describe qualitatively and quantitatively the microbiological, chemical and particulate contamination of the indoor environment of these facilities. Measurements campaigns were taken in Rennes and Nancy in summer 2018 and winter 2019 in six liberal facilities and four medico-social institutions for seniors. The first results show that indoor air contained a complex mixture of many pollutants found in rather low concentrations, below the indoor air quality guidelines.
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- 2020
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37. LE GROUPE D'EXPERTS AFNOR X43C/GE 1 « BIOAÉROSOLS » : UNE NOUVELLE DYNAMIQUE SUR LE THÈME DES BIOAÉROSOLS AU NIVEAU NATIONAL
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P. DUQUENNE, P. LE CANN, B. FACON, O. SCHLOSSER, E. DALIBERT, A. SACHOT, P. ARNOULD, and I. CHEVALIER-ALLIO
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AFNOR ,Bioaérosols ,Normalisation ,Bioaerosols ,Standardization ,French group of experts ,Groupe d'experts Français - Abstract
L'article propose un état d'avancement des travaux de normalisation au niveau européen et présente les activités et les objectifs du groupe d'experts français « Bioaérosols » nouvellement créé. Le groupe de travail européen CEN/TC 137/WG5 « Mesurage des agents biologiques » a engagé la révision des normes EN 13098, EN 14031 et EN 14583 sur les bioaérosols. Le groupe d'experts AFNOR X43C/GE 1 « Bioaérosols » a été créé en vue de suivre ces travaux européens et de forger une position Française pour la révision des normes. La création de ce groupe français génère une dynamique nouvelle et fédératrice sur le thème des bioaérosols au niveau national., The article proposes a progress report on standardization work at the European level and presents the activities and objectives of the newly created French group of experts "Bioaerosols". The European Working Group CEN/TC 137/WG 5 "Measurement of biological agents" has initiated the revision of standards EN 13098, EN 14031 and EN 14583 on bioaerosols. The expert group AFNOR X43C / GE 1 "Bioaerosols" was created to follow this European work and to forge a French position for the revision of standards. The creation of this French group generates a new and federative dynamic on the theme of bioaerosols at the national level.
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- 2020
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38. CARACTÉRISATION DU MICROBIOTE BACTÉRIEN DES POUSSIÈRES INTÉRIEURES CHEZ LES PATIENTS ASTHMATIQUES ET NON ASTHMATIQUES : UNE ÉTUDE PILOTE UTILISANT LE SÉQUENÇAGE HAUT DÉBIT
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P. LE CANN, P. LEMIRE, and J.-P. GANGNEUX
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Environmental health ,Asthme ,Poussière domestique ,Indoor air quality ,Household dust ,Asthma ,Qualité de l'air intérieur ,Santé environnementale - Abstract
L'exposition des occupants des logements aux polluants de l'air intérieur a augmenté au cours des dernières décennies. Parmi les contaminants microbiologiques, les aérosols bactériens restent cependant peu étudiés. Cette étude visait à évaluer la diversité des communautés microbiennes intérieures en relation avec la santé des occupants. Les mesures ont été effectuées sur des logements de 2 cohortes en Bretagne (France), l'une avec des enfants sans pathologie et l'autre avec des enfants et adultes asthmatiques. Trente échantillons de poussière ont été analysés par séquençage haut débit afin d'évaluer la possibilité d'étudier le rôle des communautés microbiennes intérieures dans le développement des effets sur la santé, particulièrement l'asthme. Les quatre principaux phyla bactériens ont été identifiés : Actinobactéries, Bacteroidetes, Firmicutes, et Protéobactéries avec plus de 89% de toutes les séquences. Aucun genre bactérien n'a été associé de façon significative à la gravité de l'asthme chez les enfants. Au niveau du genre, Massilia était significativement plus fréquent chez les personnes non asthmatiques.
- Published
- 2019
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39. QUALITÉ DE L'AIR INTÉRIEUR DES STRUCTURES MÉDICOSOCIALES ET LIBÉRALES
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E. BAURÈS, J.P. GANGNEUX, O. BLANCHARD, P. LE CANN, and A. FLORENTIN
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institution médicale ,spatial and temporal variability ,pollutants ,air sampling methodology ,polluants ,Indoor air ,health center ,Air intérieur ,méthodologie de prélèvement d'air ,varibilité spatio-temporelle - Abstract
La maitrise de l'environnement intérieur, dont la qualité de l'air, est un enjeu primordial du Plan National Santé Environnement 3. Dans ce contexte, les hôpitaux ont bénéficié d'études récentes d'évaluation de la qualité de l'air intérieur). Néanmoins, les autres structures de soins à la fois médico-sociale et libérale ne bénéficient que d'une exploration très limitée. Pourtant ces structures présentent des problématiques similaires tant en termes de typologie des populations que de pratiques. La plupart des polluants trouvés dans le secteur hospitalier sont utilisés dans les cabinets médicaux, dentaires ou dans les maisons de retraite, telles que les solutions hydroalcooliques, les produits pharmaceutiques, les détergents / désinfectants? ce qui peut donc exposer les résidents des maisons de retraite, les professionnels et le public. De plus, ces structures sont encore peu étudiées dans le domaine de la qualité de l'air intérieur. Comme les hôpitaux, ces structures sont des lieux de passage importants entraînant une contamination physique, chimique et microbiologique spécifique. Dans cette étude, nous nous concentrerons sur les contaminants liés aux utilisations et aux activités de ces structures. L'objectif principal du projet médiQAI est de disposer de données qualitatives et quantitatives concernant la contamination de l'environnement intérieur par des substances chimiques et des agents microbiens de différentes structures médico-sociales (EHPAD, FAS/MAS, maison de retraite) et structures libérales (cabinet de médecine en ville, cabinet dentaire, pharmacie, structure ambulatoire ...) en vue de l'évaluation de l'exposition du personnel et des patients.
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- 2019
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40. Mise en place de consultations tripartites en hématologie clinique ambulatoire : retour d’expérience à 18 mois
- Author
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Le Bozec, A., Vello, A., Hauer, S., Eftekhari, P., Le Cann, M., Rousseau, J., Legros, L., and Chaumais, M.C.
- Abstract
Depuis avril 2022, le service d’hématologie clinique ambulatoire de notre établissement réalise en lien avec le service de pharmacie clinique (PC) et une infirmière, des consultations tripartites (CT), lors de l’instauration de thérapies orales et/ou interféron puis du suivi. L’objectif de ces CT pour l’équipe pluridisciplinaire (hématologues, infirmières et pharmaciens) est d’améliorer la qualité et la sécurisation du parcours de soin des patients d’oncohématologie.
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- 2024
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41. CAR T-cell therapy in primary central nervous system lymphoma: the clinical experience of the French LOC network
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Alcantara, Marion, Houillier, Caroline, Blonski, Marie, Rubio, Marie-Thérèse, Willems, Lise, Rascalou, Agathe Waultier, Le Garff-Tavernier, Magali, Maloum, Karim, Bravetti, Clotilde, Souchet, Laetitia, Roos-Weil, Damien, Morel, Véronique, Uzunov, Madalina, Metz, Carole, Dhib-Charfi, Meriem, Nguyen, Stéphanie, Shor, Natalia, Psimaras, Dimitri, Weiss, Nicolas, Jacque, Nathalie, Solorzano, Silvia, Gauthier, Nicolas, Le Cann, Marie, Norol, Françoise, Soussain, Carole, and Choquet, Sylvain
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- 2022
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42. Integrative study of pandemic A/H1N1 influenza infections: design and methods of the CoPanFlu-France cohort
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Lapidus Nathanael, de Lamballerie Xavier, Salez Nicolas, Setbon Michel, Ferrari Pascal, Delabre Rosemary M, Gougeon Marie-Lise, Vely Frédéric, Leruez-Ville Marianne, Andreoletti Laurent, Cauchemez Simon, Boëlle Pierre-Yves, Vivier Eric, Abel Laurent, Schwarzinger Michaël, Legeas Michèle, Le Cann Pierre, Flahault Antoine, and Carrat Fabrice
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Influenza a virus H1N1 subtype ,Cohort study ,Risk factors ,France ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background The risk of influenza infection depends on biological characteristics, individual or collective behaviors and the environmental context. The Cohorts for Pandemic Influenza (CoPanFlu) France study was set up in 2009 after the identification of the novel swine-origin A/H1N1 pandemic influenza virus. This cohort of 601 households (1450 subjects) representative for the general population aims at using an integrative approach to study the risk and characteristics of influenza infection as a complex combination of data collected from questionnaires regarding sociodemographic, medical, behavioral characteristics of subjects and indoor environment, using biological samples or environmental databases. Methods/Design Households were included between December 2009 and July 2010. The design of this study relies on systematic follow-up visits between influenza seasons and additional visits during influenza seasons, when an influenza-like illness is detected in a household via an active surveillance system. During systematic visits, a nurse collects individual and environmental data on questionnaires and obtains blood samples from all members of the household. When an influenza-like-illness is detected, a nurse visits the household three times during the 12 following days, and collects data on questionnaires regarding exposure and symptoms, and biological samples (including nasal swabs) from all subjects in the household. The end of the follow-up period is expected in fall 2012. Discussion The large amount of data collected throughout the follow-up will permit a multidisciplinary study of influenza infections. Additional data is being collected and analyzed in this ongoing cohort. The longitudinal analysis of these households will permit integrative analyses of complex phenomena such as individual, collective and environmental risk factors of infection, routes of transmission, or determinants of the immune response to infection or vaccination.
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- 2012
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43. QUALITÉ DE L'AIR INTÉRIEUR DANS LES HÔPITAUX
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E. BAURÈS, J.P. GANGNEUX, O. BLANCHARD, F. MERCIER, P. LE CANN, and A. FLORENTIN
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biocontamination ,Aérosols et qualité de l’air intérieur ,spatial and temporal variability ,variabilité spatio-temporelle ,pollutants ,hôpital ,polluants ,Indoor air ,hospital ,Air intérieur - Abstract
La maitrise de la qualité de l'air est un enjeu primordial dans les environnements sensibles comme le milieu hospitalier. L'objectif du projet QAIHOSP est de disposer de données qualitatives et quantitatives sur la contamination de l'air intérieur hospitalier par des substances chimiques et des agents microbiens afin d'évaluer la variabilité spatio-temporelle de la contamination. L'étude s'est déroulée dans deux établissements de santé français. Ces campagnes ont permis l'analyse de paramètres chimiques, d'agents microbiologiques, des particules PM 2,5 et 10 et en parallèle, des paramètres de confort. Lors de ces campagnes, une pollution plutôt faible de l'air intérieur a été analysée dans les deux hôpitaux. Ceci est principalement dû à une ventilation double-flux qui induit un renouvellement d'air important. Une variabilité spatiale a été observée principalement en liens avec les activités de soins et usages. Les profils de contamination sont similaires entre Rennes et Nancy. L'activité semble plus déterminer le profil de pollution que la géographie et surtout le renouvellement d'air qui montre son efficacité., Indoor air quality control is a very important issue in the sensitive environments as the hospital. The objective of the QAIHOSP project is to have qualitative and quantitative data on the contamination of the hospital indoor air by chemical substances and microbial agents. The aim is to estimate the spatial and temporal variability of the contamination. The study took place in two French establishments of health. These campaigns allowed to analyse chemical parameters, microbiological agents and particles PM 2.5 and PM10 and in parallel, to measure the ambiant parameters. Concentrations were found in the same order of magnitude in both hospitals. However, the results have shown that indoor air contains a complex mixture of chemical, physical and microbiological compounds. Compared to indoor air in dwellings, our study shows low, at least equivalent, contamination for non-hospital specific parameters, which is related to effective ventilation. Chemical compounds retrieved at the highest concentration and most frequently are due to health care activities and uses.
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- 2018
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44. Spatio-temporal evolution of hydroxyapatite crystal thickness at the bone-implant interface.
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Le Cann, Sophie, Törnquist, Elin, Silva Barreto, Isabella, Fraulob, Manon, Albini Lomami, Hugues, Verezhak, Mariana, Guizar-Sicairos, Manuel, Isaksson, Hanna, and Haïat, Guillaume
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SMALL-angle X-ray scattering ,MINERALS ,BONES ,BONE growth ,INTERFACIAL stresses - Abstract
A better understanding of bone nanostructure around the bone-implant interface is essential to improve longevity of clinical implants and decrease failure risks. This study investigates the spatio-temporal evolution of mineral crystal thickness and plate orientation in newly formed bone around the surface of a metallic implant. Standardized coin-shaped titanium implants designed with a bone chamber were inserted into rabbit tibiae for 7 and 13 weeks. Scanning measurements with micro-focused small-angle X-ray scattering (SAXS) were carried out on newly formed bone close to the implant and in control mature cortical bone. Mineral crystals were thinner close to the implant (1.8 ± 0.45 nm at 7 weeks and 2.4 ± 0.57 nm at 13 weeks) than in the control mature bone tissue (2.5 ± 0.21 nm at 7 weeks and 2.8 ± 0.35 nm at 13 weeks), with increasing thickness over healing time (+30 % in 6 weeks). These results are explained by younger bone close to the implant, which matures during osseointegration. Thinner mineral crystals parallel to the implant surface within the first 100 µm indicate that the implant affects the ultrastructure of neighbouring bone , potentially due to heterogeneous interfacial stresses, and suggest a longer maturation process of bone tissue and difficulty in binding to the metal. The bone growth kinetics within the bone chamber was derived from the spatio-temporal evolution of bone tissue's nanostructure, coupled with microtomographic imaging. The findings indicate that understanding mineral crystal thickness or plate orientation can improve our knowledge of osseointegration. Image, graphical abstract [ABSTRACT FROM AUTHOR]
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- 2020
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45. Assessment of poly-<scp>l</scp> -lysine dendrigrafts for virus concentration in water: use of MS2 bacteriophage as proof of concept
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A. Gérard, Benoit Couturaud, J. Boismard, A. Mas, P. Le Cann, Clément Faye, Benoit Roig, Axelle Cadiere, Laurent Garrelly, Laboratoire d'étude et de recherche en environnement et santé (LERES), École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Colcom SARL, Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)
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Concentration ,Real-Time Polymerase Chain Reaction ,Applied Microbiology and Biotechnology ,Virus ,Microbiology ,law.invention ,Bacteriophage ,03 medical and health sciences ,law ,Diagnosis ,Water safety ,Bacteriophage MS2 ,Polylysine ,Filtration ,Levivirus ,030304 developmental biology ,0303 health sciences ,Chromatography ,Poly-l-lysine dendrigrafts ,biology ,030306 microbiology ,Extraction (chemistry) ,General Medicine ,Contamination ,biology.organism_classification ,[CHIM.POLY]Chemical Sciences/Polymers ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,13. Climate action ,Nucleic acid ,Water Microbiology ,Water use ,Biotechnology - Abstract
Aims Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly-l-lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real-time PCR quantification. Methods and Results This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0·99 ± 0·04 and Y intercept when x = −0·02 ± 0·28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16 600-fold 1 l of sample and to detect and quantify down to 750 GC l−1 and 7500 GC l−1, respectively). Conclusions To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water. Significance and Impact of the Study This study gives valuable advance in the methods of concentration and diagnosis of virus in water.
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- 2013
46. Rapid quantification of viable fungi in hospital environments: analysis of air and surface samples using solid-phase cytometry
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P. Le Cann, Jean-Pierre Gangneux, D. Meheust, Laboratoire d'étude et de recherche en environnement et santé (LERES), École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
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MESH: Fungi ,Microbiology (medical) ,Veterinary medicine ,Air sampling ,Fungal contamination ,Air Microbiology ,Colony Count, Microbial ,Air sampler ,010501 environmental sciences ,Biology ,01 natural sciences ,Microbiology ,Hospital ,03 medical and health sciences ,MESH: Air Microbiology ,Environmental Microbiology ,Humans ,Malt extract agar ,MESH: Colony Count, Microbial ,Image Cytometry ,MESH: Microbial Viability ,0105 earth and related environmental sciences ,Viable fungi ,0303 health sciences ,Microbial Viability ,MESH: Humans ,030306 microbiology ,Environmental surveillance ,Solid-phase cytometry ,fungi ,Significant difference ,Fungi ,MESH: Environmental Microbiology ,General Medicine ,MESH: Hospitals ,Hospitals ,3. Good health ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Infectious Diseases ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Infectious risk ,Cytometry ,MESH: Image Cytometry - Abstract
International audience; BACKGROUND: Environmental surveillance is important in high-risk areas of hospitals to prevent fungal infections in immunosuppressed patients. Conventional culture methods for enumerating environmental fungi are time-consuming. AIM: In this field study, a solid-phase cytometry technique (SPC) and a more conventional culture-based method to quantify fungal contamination of hospital air and surface samples were compared. METHODS: For the air sampling, a liquid cyclone air sampler was used with a flow rate of 300 L/min for 10 min in each of four hospital locations. Surface swabbing was done in two locations, with two different swab types. Samples from all areas were processed by SPC and by culture on malt extract agar. FINDINGS: The mean airborne concentrations of viable fungi determined by SPC were about 1.5-fold higher than the mean concentrations obtained with the culture-based method. These differences for air samples were significant in three hospital environments. No significant difference was observed for surface samples between the two swab types and between the two analytical methods. One of the prominent advantages of SPC was its rapidity in comparison with the culture-based method (5 h versus 5 days). CONCLUSION: In this study, we showed that SPC allows for rapid monitoring of viable fungi in hospital environments. SPC can thus be used to provide an early warning and a rapid implementation of corrective measures. Viable fungi detection may be an important tool to assess infectious risk in wards with immunosuppressed patients.
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- 2013
47. Analyse physicochimique et microbiologique de la Qualité de l’Air Intérieur dans les établissements HOSPitaliers : l’étude QAIHOSP
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P. Le Cann, S. Belaz, Jean-Pierre Gangneux, Fabien Mercier, Alexandre Rivier, Arnaud Florentin, P. Y. Donnio, Estelle Baurès, Olivier Blanchard, Monique Guillaso, E. Surget, CHU Pontchaillou [Rennes], Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Service de Parasitologie-Mycologie [Rennes], Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], École des Hautes Études en Santé Publique [EHESP] (EHESP), Département Santé Environnement Travail et Génie Sanitaire (DSETGS), Laboratoire d'étude et de recherche en environnement et santé (LERES), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université d'Angers (UA), Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes]
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0303 health sciences ,03 medical and health sciences ,Qualité de l’Air Intérieur ,0302 clinical medicine ,Infectious Diseases ,030306 microbiology ,Etablissements Hospitaliers ,[SDV]Life Sciences [q-bio] ,030231 tropical medicine ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,ComputingMilieux_MISCELLANEOUS - Abstract
Introduction L’objectif de cette etude est de disposer de donnees qualitatives et quantitatives sur la contamination de l’environnement interieur hospitalier, microbiologique et chimique, afin d’evaluer l’exposition du personnel, des visiteurs et des patients et la variabilite spatio-temporelle de la contamination. Methodes L’etude s’est deroulee dans differents lieux des CHU de Rennes et de Nancy : hall d’accueil, salle de soins infirmiers, salle de reveil post-operatoire, chambre d’un patient, unite de desinfection des endoscopes, laboratoire de parasitologie et salle de decoupe de plâtres. Deux campagnes de prelevement ont eu lieu en ete 2014 et en hiver 2015. Les methodes de prelevement etaient nombreuses et complementaires ( Fig. 1 ). L’analyse des donnees a ete realisee sous IBM SPSS grâce au test de Mann-Whitney et le coefficient de correlation de Pearson. Resultats Les concentrations d’aldehydes, de composes organiques volatils (COV) et semi-volatils etaient faibles a tres faibles dans les 2 etablissements. Etaient detectables, mais sans differences globales entre les 2 etablissements : la contamination fongique (m = 226 UFC/m3 ; p = 0,97) ou bacterienne (m = 352 UFC/m3 ; p = 0,14), les particules PM2,5 (m = 2,1 μg/m3 ; p = 0,97) et PM10 (m = 6,6 μg/m3 ; p = 0,84). Sur le plan saisonnier, la contamination fongique etait plus importante en ete pour les 2 etablissements (p = 0,002). La contamination bacterienne ne varie pas significativement entre les locaux (p = 0,14) contrairement a la contamination fongique (p = 0,02) et particulaires PM2,5 (p = 0,01) ou PM10 (p = 0,01). Cette derniere est plus importante dans le hall (m = 879 UFC/m3), le laboratoire de parasitologie (m = 333 UFC/m3) et la salle de plâtre (m = 310 UFC/m3). Nous ne retrouvons pas de correlation significative entre le nombre de personnes, le taux de CO2, l’humidite relative et le denombrement bacterien ou fongique. Neanmoins nous retrouvons une relation forte entre la temperature du local et la contamination bacterienne (r = 0,7, p = 0,008) ou la contamination fongique (r = 0,56, p = 0,045) d’une part, et entre la contamination fongique et les PM10 (r = 0,52, p = 0,022) d’autre part. Conclusion Notre etude montre une tres faible contamination par les aldehydes, COV et sCOV comparativement a d’autres lieux publics. La forte variabilite spatio-temporelle de la contamination fongique et particulaire est liee a la saison, a l’activite et a la ventilation.
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- 2016
48. Assessing the impact of pain-linked Nav1.7 variants: An example of two variants with no biophysical effect
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Le Cann, Kim, Meents, Jannis E., Sudha Bhagavath Eswaran, Vishal, Dohrn, Maike F., Bott, Raya, Maier, Andrea, Bialer, Martin, Hautvast, Petra, Erickson, Andelain, Rolke, Roman, Rothermel, Markus, Körner, Jannis, Kurth, Ingo, and Lampert, Angelika
- Abstract
ABSTRACTMutations in the voltage-gated sodium channel Nav1.7 are linked to human pain. The Nav1.7/N1245S variant was described before in several patients suffering from primary erythromelalgia and/or olfactory hypersensitivity. We have identified this variant in a pain patient and a patient suffering from severe and life-threatening orthostatic hypotension. In addition, we report a female patient suffering from muscle pain and carrying the Nav1.7/E1139K variant. We tested both Nav1.7 variants by whole-cell voltage-clamp recordings in HEK293 cells, revealing a slightly enhanced current density for the N1245S variant when co-expressed with the β1 subunit. This effect was counteracted by an enhanced slow inactivation. Both variants showed similar voltage dependence of activation and steady-state fast inactivation, as well as kinetics of fast inactivation, deactivation, and use-dependency compared to WT Nav1.7. Finally, homology modeling revealed that the N1245S substitution results in different intramolecular interaction partners. Taken together, these experiments do not point to a clear pathogenic effect of either the N1245S or E1139K variant and suggest they may not be solely responsible for the patients’ pain symptoms. As discussed previously for other variants, investigations in heterologous expression systems may not sufficiently mimic the pathophysiological situation in pain patients, and single nucleotide variants in other genes or modulatory proteins are necessary for these specific variants to show their effect. Our findings stress that biophysical investigations of ion channel mutations need to be evaluated with care and should preferably be supplemented with studies investigating the mutations in their context, ideally in human sensory neurons.
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- 2021
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49. CANOMAD: a neurological monoclonal gammopathy of clinical significance that benefits from B-cell–targeted therapies
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Le Cann, Marie, Bouhour, Françoise, Viala, Karine, Simon, Laurence, Tard, Céline, Rossi, Cédric, Morel, Guillaume, Lagrange, Emmeline, Magy, Laurent, Créange, Alain, Michaud, Maud, Franques, Jérôme, Echaniz-Laguna, Andoni, Antoine, Jean-Christophe, Baron, Marine, Arnulf, Bertrand, Puma, Angela, Delmont, Emilien, Maisonobe, Thierry, Leblond, Véronique, and Roos-Weil, Damien
- Abstract
CANOMAD (chronic ataxic neuropathy, ophthalmoplegia, immunoglobulin M [IgM] paraprotein, cold agglutinins, and disialosyl antibodies) is a rare syndrome characterized by chronic neuropathy with sensory ataxia, ocular, and/or bulbar motor weakness in the presence of a monoclonal IgM reacting against gangliosides containing disialosyl epitopes. Data regarding associated hematologic malignancies and effective therapies in CANOMAD are scarce. We conducted a French multicenter retrospective study that included 45 patients with serum IgM antibodies reacting against disialosyl epitopes in the context of evocating neurologic symptoms. The main clinical features were sensitive symptoms (ataxia, paresthesia, hypoesthesia; n = 45, 100%), motor weakness (n = 18, 40%), ophthalmoplegia (n = 20, 45%), and bulbar symptoms (n = 6, 13%). Forty-five percent of the cohort had moderate to severe disability (modified Rankin score, 3-5). Cold agglutinins were identified in 15 (34%) patients. Electrophysiologic studies showed a demyelinating or axonal pattern in, respectively, 60% and 27% of cases. All patients had serum monoclonal IgM gammopathy (median, 2.6 g/L; range, 0.1-40 g/L). Overt hematologic malignancies were diagnosed in 16 patients (36%), with the most frequent being Waldenström macroglobulinemia (n = 9, 20%). Forty-one patients (91%) required treatment of CANOMAD. Intravenous immunoglobulins (IVIg) and rituximab-based regimens were the most effective therapies with, respectively, 53% and 52% of partial or better clinical responses. Corticosteroids and immunosuppressive drugs were largely ineffective. Although more studies are warranted to better define the optimal therapeutic sequence, IVIg should be proposed as the standard of care for first-line treatment and rituximab-based regimens for second-line treatment. These compiled data argue for CANOMAD to be included in neurologic monoclonal gammopathy of clinical significance.
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- 2020
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50. Real-time RT-PCR for norovirus screening in shellfish
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Patrice Guillon, Monique Pommepuy, P. Le Cann, Fabienne Loisy, Robert L. Atmar, and F. Le Guyader
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Reverse Transcriptase Polymerase Chain Reaction ,viruses ,Norovirus ,virus diseases ,Large range ,Biology ,Amplicon ,medicine.disease_cause ,Ostreidae ,Sensitivity and Specificity ,Virology ,digestive system diseases ,Microbiology ,fluids and secretions ,Real-time polymerase chain reaction ,medicine ,Animals ,RNA, Viral ,Shellfish ,DNA Primers - Abstract
Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II. Seventy and seven RT-PCR units of genogroup I and II reference norovirus strains, respectively, were detected in artificially contaminated oysters. Validation of the new method on 150 archived naturally contaminated shellfish confirmed the utility of the genogroup II primer set to detect a large range of different strains circulating in France since 1995, but genogroup I strains were detected infrequently.
- Published
- 2005
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