1. Cryopreservation of Pelargonium apices by droplet-vitrification
- Author
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Gallard, A., Panis, B., Dorion, N., Swennen, R., Agnes Grapin, Génétique et Horticulture (GenHort), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Unité mixte de recherche génétique et horticulture Genhort, Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-Institut National d'Horticulture, Université Catholique de Louvain = Catholic University of Louvain (UCL), ProdInra, Migration, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
- Subjects
Cryopreservation ,PELARGONIUM ,AZOTE LIQUIDE ,[SDV]Life Sciences [q-bio] ,MESH: Freeze Drying ,MESH: Cryoprotective Agents ,P X HORTORUM ,VITRIFICATION ,[SDV] Life Sciences [q-bio] ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,Cryoprotective Agents ,Freeze Drying ,MESH: Seeds ,LS ,Seeds ,MESH: Cryopreservation ,PVS2 ,TAUX DE SURVIE ,MESH: Pelargonium ,P X PELTATUM - Abstract
International audience; The droplet-vitrification method was adapted to Pelargonium apices by optimizing the duration of the loading solution (LS) as well as the plant vitrification solution 2 (PVS2). The excised apices were dehydrated in two steps (20 min in LS and 15 min in PVS2) and then immersed directly in liquid nitrogen (LN). After thawing and unloading in the recovery solution at room temperature for 15 min, apices were plated onto semi-solid Murashige and Skoog medium. This simple protocol without any pretreatment was successfully applied to eight cultivars with a survival level ranging between 55.6 - 96.2 % and a regrowth level between 9.1% - 70.6%. These results prove the feasibility of the long-term storage of Pelargonium germplasm through cryopreservation
- Published
- 2008