416 results on '"P Seshadri S"'
Search Results
2. Synthesis, growth and characterization of nonlinear optical crystal: propylenediamine picrate (PDP) single crystals
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Raja, R., Seshadri, S., and Santhanam, V.
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- 2024
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3. Grip Strength, Gait Speed and Plasma Markers of Neurodegeneration in Asymptomatic Middle-aged and Older Adults
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Jacob, Mini E., O’Donnell, A., Samra, J., Gonzales, M. M., Satizabal, C., Pase, M. P., Murabito, J. M., Beiser, A., and Seshadri, S.
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- 2022
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4. Characterisation and natural progression of SARS-CoV-2 infection in ferrets
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Au, Gough G., Marsh, Glenn A., McAuley, Alexander J., Lowther, Suzanne, Trinidad, Lee, Edwards, Sarah, Todd, Shawn, Barr, Jennifer, Bruce, Matthew P., Poole, Timothy B., Brown, Sheree, Layton, Rachel, Riddell, Sarah, Rowe, Brenton, Soldani, Elisha, Suen, Willy W., Bergfeld, Jemma, Bingham, John, Payne, Jean, Durr, Peter A., Drew, Trevor W., and Vasan, Seshadri S.
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- 2022
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5. Characterisation and natural progression of SARS-CoV-2 infection in ferrets
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Gough G. Au, Glenn A. Marsh, Alexander J. McAuley, Suzanne Lowther, Lee Trinidad, Sarah Edwards, Shawn Todd, Jennifer Barr, Matthew P. Bruce, Timothy B. Poole, Sheree Brown, Rachel Layton, Sarah Riddell, Brenton Rowe, Elisha Soldani, Willy W. Suen, Jemma Bergfeld, John Bingham, Jean Payne, Peter A. Durr, Trevor W. Drew, and Seshadri S. Vasan
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Medicine ,Science - Abstract
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the infectious disease COVID-19, which has rapidly become an international pandemic with significant impact on healthcare systems and the global economy. To assist antiviral therapy and vaccine development efforts, we performed a natural history/time course study of SARS-CoV-2 infection in ferrets to characterise and assess the suitability of this animal model. Ten ferrets of each sex were challenged intranasally with 4.64 × 104 TCID50 of SARS-CoV-2 isolate Australia/VIC01/2020 and monitored for clinical disease signs, viral shedding, and tissues collected post-mortem for histopathological and virological assessment at set intervals. We found that SARS-CoV-2 replicated in the upper respiratory tract of ferrets with consistent viral shedding in nasal wash samples and oral swab samples up until day 9. Infectious SARS-CoV-2 was recovered from nasal washes, oral swabs, nasal turbinates, pharynx, and olfactory bulb samples within 3–7 days post-challenge; however, only viral RNA was detected by qRT-PCR in samples collected from the trachea, lung, and parts of the gastrointestinal tract. Viral antigen was seen exclusively in nasal epithelium and associated sloughed cells and draining lymph nodes upon immunohistochemical staining. Due to the absence of clinical signs after viral challenge, our ferret model is appropriate for studying asymptomatic SARS-CoV-2 infections and most suitable for use in vaccine efficacy studies.
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- 2022
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6. Senolytic Therapy to Modulate the Progression of Alzheimer’s Disease (SToMP-AD): A Pilot Clinical Trial
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Gonzales, Mitzi M., Garbarino, V. R., Marques Zilli, E., Petersen, R. C., Kirkland, J. L., Tchkonia, T., Musi, N., Seshadri, S., Craft, S., and Orr, Miranda E.
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- 2022
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7. BMIM[BF4]: An Efficient Ionic Liquid Medium for the Synthesis of Chromeno[b]pyridines as Potential Anticancer Agents
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Kalpana, K., Rani, V. A., Seshadri, S., and Kiran, B. Ravi
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- 2021
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8. Experimental Program of the HL-LHC Inner Triplet String Test at CERN
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Yammine, S., Bajko, M., Ballarino, A., Bednarek, M. J., Bozzini, D., Blanchard, S., Bruning, O., Cruikshank, P., Denz, R., Ramos, D. Duarte, Fleiter, J., Gamba, D., Garcia, Nicolas Heredia, Herty, A., Leclercq, Y., Maan, W., Martino, M., Modena, M., Onufrena, A., Perin, A., Pojer, M., Prin, H., Ravaioli, E., Mateos, Felix, Seshadri, S., Steckert, J., Thiesen, H., Todesco, E., Tomas Garcia, Rogelio, Verweij, A., Wollmann, D., and Zerlauth, M.
- Abstract
This contribution describes the experimental program already undergoing and to be completed on the High Luminosity Large Hadron Collider (HL-LHC) Inner Triplet (IT) String, an important intermediate milestone of the HL-LHC project at CERN. First, it describes the magnet circuits of the HL-LHC IT String. Afterwards, the different systems installed to perform the experimental program are detailed. The proposed tests are defined for the validation of the cryogenic system, the full remote alignment system, the powering system, and the protection schemes of all magnets working in unison. This strategy will allow for a verification of the integrated powering system before the final installation and commissioning in the HL-LHC's underground areas.
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- 2024
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9. At Least Three Doses of Leading Vaccines Essential for Neutralisation of SARS-CoV-2 Omicron Variant
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Nagendrakumar B. Singanallur, Petrus Jansen van Vuren, Alexander J. McAuley, Matthew P. Bruce, Michael J. Kuiper, Stella M. Gwini, Shane Riddell, Sarah Goldie, Trevor W. Drew, Kim R. Blasdell, Mary Tachedjian, Shruthi Mangalaganesh, Simran Chahal, Leon Caly, Julian D. Druce, Jennifer A. Juno, Stephen J. Kent, Adam K. Wheatley, and Seshadri S. Vasan
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AlphaFold ,biomolecular modelling ,COVID-19 ,neutralising antibody titres ,SARS-CoV-2 ,spike protein ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Plasma samples taken at different time points from donors who received either AstraZeneca (Vaxzevria) or Pfizer (Comirnaty) or Moderna (Spikevax) coronavirus disease-19 (COVID-19) vaccine were assessed in virus neutralization assays against Delta and Omicron variants of concern and a reference isolate (VIC31). With the Pfizer vaccine there was 6-8-fold reduction in 50% neutralizing antibody titres (NT50) against Delta and VIC31 at 6 months compared to 2 weeks after the second dose; followed by 25-fold increase at 2 weeks after the third dose. Neutralisation of Omicron was only consistently observed 2 weeks after the third dose, with most samples having titres below the limit of detection at earlier timepoints. Moderna results were similar to Pfizer at 2 weeks after the second dose, while the titres for AstraZeneca samples derived from older donors were 7-fold lower against VIC31 and below the limit of detection against Delta and Omicron. Age and gender were not found to significantly impact our results. These findings indicate that vaccine matching may be needed, and that at least a third dose of these vaccines is necessary to generate sufficient neutralising antibodies against emerging variants of concern, especially Omicron, amidst the challenges of ensuring vaccine equity worldwide.
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- 2022
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10. Genetic overlap between Alzheimer’s disease and Parkinson’s disease at the MAPT locus
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Desikan, RS, Schork, AJ, Wang, Y, Witoelar, A, Sharma, M, McEvoy, LK, Holland, D, Brewer, JB, Chen, C-H, Thompson, WK, Harold, D, Williams, J, Owen, MJ, O'Donovan, MC, Pericak-Vance, MA, Mayeux, R, Haines, JL, Farrer, LA, Schellenberg, GD, Heutink, P, Singleton, AB, Brice, A, Wood, NW, Hardy, J, Martinez, M, Choi, SH, DeStefano, A, Ikram, MA, Bis, JC, Smith, A, Fitzpatrick, AL, Launer, L, van Duijn, C, Seshadri, S, Ulstein, ID, Aarsland, D, Fladby, T, Djurovic, S, Hyman, BT, Snaedal, J, Stefansson, H, Stefansson, K, Gasser, T, Andreassen, OA, and Dale, AM
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Biomedical and Clinical Sciences ,Biological Psychology ,Clinical and Health Psychology ,Clinical Sciences ,Psychology ,Human Genome ,Brain Disorders ,Neurosciences ,Neurodegenerative ,Parkinson's Disease ,Prevention ,Aging ,Alzheimer's Disease ,Dementia ,Genetics ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Acquired Cognitive Impairment ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,Aged ,Aged ,80 and over ,Alleles ,Alzheimer Disease ,Apolipoproteins E ,Brain ,Chromosomes ,Human ,Pair 17 ,Female ,Genetic Loci ,Genetic Pleiotropy ,Genome-Wide Association Study ,Humans ,Male ,Middle Aged ,Parkinson Disease ,Polymorphism ,Single Nucleotide ,tau Proteins ,ADNI ,ADGC ,GERAD ,CHARGE and IPDGC Investigators ,Biological Sciences ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Psychiatry ,Clinical sciences ,Biological psychology ,Clinical and health psychology - Abstract
We investigated the genetic overlap between Alzheimer's disease (AD) and Parkinson's disease (PD). Using summary statistics (P-values) from large recent genome-wide association studies (GWAS) (total n=89 904 individuals), we sought to identify single nucleotide polymorphisms (SNPs) associating with both AD and PD. We found and replicated association of both AD and PD with the A allele of rs393152 within the extended MAPT region on chromosome 17 (meta analysis P-value across five independent AD cohorts=1.65 × 10(-7)). In independent datasets, we found a dose-dependent effect of the A allele of rs393152 on intra-cerebral MAPT transcript levels and volume loss within the entorhinal cortex and hippocampus. Our findings identify the tau-associated MAPT locus as a site of genetic overlap between AD and PD, and extending prior work, we show that the MAPT region increases risk of Alzheimer's neurodegeneration.
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- 2015
11. Advances and gaps in SARS-CoV-2 infection models.
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César Muñoz-Fontela, Lina Widerspick, Randy A Albrecht, Martin Beer, Miles W Carroll, Emmie de Wit, Michael S Diamond, William E Dowling, Simon G P Funnell, Adolfo García-Sastre, Nora M Gerhards, Rineke de Jong, Vincent J Munster, Johan Neyts, Stanley Perlman, Douglas S Reed, Juergen A Richt, Ximena Riveros-Balta, Chad J Roy, Francisco J Salguero, Michael Schotsaert, Lauren M Schwartz, Robert A Seder, Joaquim Segalés, Seshadri S Vasan, Ana María Henao-Restrepo, and Dan H Barouch
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
The global response to Coronavirus Disease 2019 (COVID-19) is now facing new challenges such as vaccine inequity and the emergence of SARS-CoV-2 variants of concern (VOCs). Preclinical models of disease, in particular animal models, are essential to investigate VOC pathogenesis, vaccine correlates of protection and postexposure therapies. Here, we provide an update from the World Health Organization (WHO) COVID-19 modeling expert group (WHO-COM) assembled by WHO, regarding advances in preclinical models. In particular, we discuss how animal model research is playing a key role to evaluate VOC virulence, transmission and immune escape, and how animal models are being refined to recapitulate COVID-19 demographic variables such as comorbidities and age.
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- 2022
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12. A Stabilized, Monomeric, Receptor Binding Domain Elicits High-Titer Neutralizing Antibodies Against All SARS-CoV-2 Variants of Concern
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Shahbaz Ahmed, Mohammad Suhail Khan, Savitha Gayathri, Randhir Singh, Sahil Kumar, Unnatiben Rajeshbhai Patel, Sameer Kumar Malladi, Raju S. Rajmani, Petrus Jansen van Vuren, Shane Riddell, Sarah Goldie, Nidhi Girish, Poorvi Reddy, Aditya Upadhyaya, Suman Pandey, Samreen Siddiqui, Akansha Tyagi, Sujeet Jha, Rajesh Pandey, Oyahida Khatun, Rohan Narayan, Shashank Tripathi, Alexander J. McAuley, Nagendrakumar Balasubramanian Singanallur, Seshadri S. Vasan, Rajesh P. Ringe, and Raghavan Varadarajan
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stabilizing mutation ,hyperstable mutants ,neutralizing antibodies ,hamster immunization ,vaccination ,lyophilization ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Saturation suppressor mutagenesis was used to generate thermostable mutants of the SARS-CoV-2 spike receptor-binding domain (RBD). A triple mutant with an increase in thermal melting temperature of ~7°C with respect to the wild-type B.1 RBD and was expressed in high yield in both mammalian cells and the microbial host, Pichia pastoris, was downselected for immunogenicity studies. An additional derivative with three additional mutations from the B.1.351 (beta) isolate was also introduced into this background. Lyophilized proteins were resistant to high-temperature exposure and could be stored for over a month at 37°C. In mice and hamsters, squalene-in-water emulsion (SWE) adjuvanted formulations of the B.1-stabilized RBD were considerably more immunogenic than RBD lacking the stabilizing mutations and elicited antibodies that neutralized all four current variants of concern with similar neutralization titers. However, sera from mice immunized with the stabilized B.1.351 derivative showed significantly decreased neutralization titers exclusively against the B.1.617.2 (delta) VOC. A cocktail comprising stabilized B.1 and B.1.351 RBDs elicited antibodies with qualitatively improved neutralization titers and breadth relative to those immunized solely with either immunogen. Immunized hamsters were protected from high-dose viral challenge. Such vaccine formulations can be rapidly and cheaply produced, lack extraneous tags or additional components, and can be stored at room temperature. They are a useful modality to combat COVID-19, especially in remote and low-resource settings.
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- 2021
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13. CoviRx: A User-Friendly Interface for Systematic Down-Selection of Repurposed Drug Candidates for COVID-19
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Hardik A. Jain, Vinti Agarwal, Chaarvi Bansal, Anupama Kumar, Faheem, Muzaffar-Ur-Rehman Mohammed, Sankaranarayanan Murugesan, Moana M. Simpson, Avinash V. Karpe, Rohitash Chandra, Christopher A. MacRaild, Ian K. Styles, Amanda L. Peterson, Matthew A. Cooper, Carl M. J. Kirkpatrick, Rohan M. Shah, Enzo A. Palombo, Natalie L. Trevaskis, Darren J. Creek, and Seshadri S. Vasan
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COVID-19 ,drug fingerprints ,drug repurposing ,open-source dataset ,search engine ,web application development ,Bibliography. Library science. Information resources - Abstract
Although various vaccines are now commercially available, they have not been able to stop the spread of COVID-19 infection completely. An excellent strategy to get safe, effective, and affordable COVID-19 treatments quickly is to repurpose drugs that are already approved for other diseases. The process of developing an accurate and standardized drug repurposing dataset requires considerable resources and expertise due to numerous commercially available drugs that could be potentially used to address the SARS-CoV-2 infection. To address this bottleneck, we created the CoviRx.org platform. CoviRx is a user-friendly interface that allows analysis and filtering of large quantities of data, which is onerous to curate manually for COVID-19 drug repurposing. Through CoviRx, the curated data have been made open source to help combat the ongoing pandemic and encourage users to submit their findings on the drugs they have evaluated, in a uniform format that can be validated and checked for integrity by authenticated volunteers. This article discusses the various features of CoviRx, its design principles, and how its functionality is independent of the data it displays. Thus, in the future, this platform can be extended to include any other disease beyond COVID-19.
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- 2022
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14. A Time-Series Metabolomic Analysis of SARS-CoV-2 Infection in a Ferret Model
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Avinash V. Karpe, Thao V. Nguyen, Rohan M. Shah, Gough G. Au, Alexander J. McAuley, Glenn A. Marsh, Sarah Riddell, Seshadri S. Vasan, and David J. Beale
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COVID19 ,SARS-CoV-2 ,metabolomics ,omics ,animal models ,ferret ,Microbiology ,QR1-502 - Abstract
The global threat of COVID-19 has led to an increased use of metabolomics to study SARS-CoV-2 infections in animals and humans. In spite of these efforts, however, understanding the metabolome of SARS-CoV-2 during an infection remains difficult and incomplete. In this study, metabolic responses to a SAS-CoV-2 challenge experiment were studied in nasal washes collected from an asymptomatic ferret model (n = 20) at different time points before and after infection using an LC-MS-based metabolomics approach. A multivariate analysis of the nasal wash metabolome data revealed several statistically significant features. Despite no effects of sex or interaction between sex and time on the time course of SARS-CoV-2 infection, 16 metabolites were significantly different at all time points post-infection. Among these altered metabolites, the relative abundance of taurine was elevated post-infection, which could be an indication of hepatotoxicity, while the accumulation of sialic acids could indicate SARS-CoV-2 invasion. Enrichment analysis identified several pathways influenced by SARS-CoV-2 infection. Of these, sugar, glycan, and amino acid metabolisms were the key altered pathways in the upper respiratory channel during infection. These findings provide some new insights into the progression of SARS-CoV-2 infection in ferrets at the metabolic level, which could be useful for the development of early clinical diagnosis tools and new or repurposed drug therapies.
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- 2022
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15. Animal models for COVID-19
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Muñoz-Fontela, César, Dowling, William E., Funnell, Simon G. P., Gsell, Pierre-S., Riveros-Balta, A. Ximena, Albrecht, Randy A., Andersen, Hanne, Baric, Ralph S., Carroll, Miles W., Cavaleri, Marco, Qin, Chuan, Crozier, Ian, Dallmeier, Kai, de Waal, Leon, de Wit, Emmie, Delang, Leen, Dohm, Erik, Duprex, W. Paul, Falzarano, Darryl, Finch, Courtney L., Frieman, Matthew B., Graham, Barney S., Gralinski, Lisa E., Guilfoyle, Kate, Haagmans, Bart L., Hamilton, Geraldine A., Hartman, Amy L., Herfst, Sander, Kaptein, Suzanne J. F., Klimstra, William B., Knezevic, Ivana, Krause, Philip R., Kuhn, Jens H., Le Grand, Roger, Lewis, Mark G., Liu, Wen-Chun, Maisonnasse, Pauline, McElroy, Anita K., Munster, Vincent, Oreshkova, Nadia, Rasmussen, Angela L., Rocha-Pereira, Joana, Rockx, Barry, Rodríguez, Estefanía, Rogers, Thomas F., Salguero, Francisco J., Schotsaert, Michael, Stittelaar, Koert J., Thibaut, Hendrik Jan, Tseng, Chien-Te, Vergara-Alert, Júlia, Beer, Martin, Brasel, Trevor, Chan, Jasper F. W., García-Sastre, Adolfo, Neyts, Johan, Perlman, Stanley, Reed, Douglas S., Richt, Juergen A., Roy, Chad J., Segalés, Joaquim, Vasan, Seshadri S., Henao-Restrepo, Ana María, and Barouch, Dan H.
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- 2020
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16. Soft magnetic properties of Co–Ni–Fe–Gd/Cu electrodeposited thin film
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Suresh, R., Sathishkumar, P., Kumaravelan, S., Suganthi, Nachimuthu, and Seshadri, S.
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- 2020
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17. Use of Human Lung Tissue Models for Screening of Drugs against SARS-CoV-2 Infection
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Alexander J. McAuley, Petrus Jansen van Vuren, Muzaffar-Ur-Rehman Mohammed, Faheem, Sarah Goldie, Shane Riddell, Nathan J. Gödde, Ian K. Styles, Matthew P. Bruce, Simran Chahal, Stephanie Keating, Kim R. Blasdell, Mary Tachedjian, Carmel M. O’Brien, Nagendrakumar Balasubramanian Singanallur, John Noel Viana, Aditya V. Vashi, Carl M. Kirkpatrick, Christopher A. MacRaild, Rohan M. Shah, Elizabeth Vincan, Eugene Athan, Darren J. Creek, Natalie L. Trevaskis, Sankaranarayanan Murugesan, Anupama Kumar, and Seshadri S. Vasan
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COVID-19 ,CoviRx.org ,therapeutics ,drug repurposing ,3D tissue models ,Microbiology ,QR1-502 - Abstract
The repurposing of licenced drugs for use against COVID-19 is one of the most rapid ways to develop new and alternative therapeutic options to manage the ongoing pandemic. Given circa 7817 licenced compounds available from Compounds Australia that can be screened, this paper demonstrates the utility of commercially available ex vivo/3D airway and alveolar tissue models. These models are a closer representation of in vivo studies than in vitro models, but retain the benefits of rapid in vitro screening for drug efficacy. We demonstrate that several existing drugs appear to show anti-SARS-CoV-2 activity against both SARS-CoV-2 Delta and Omicron Variants of Concern in the airway model. In particular, fluvoxamine, as well as aprepitant, everolimus, and sirolimus, has virus reduction efficacy comparable to the current standard of care (remdesivir, molnupiravir, nirmatrelvir). Whilst these results are encouraging, further testing and efficacy studies are required before clinical use can be considered.
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- 2022
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18. Systematic Down-Selection of Repurposed Drug Candidates for COVID-19
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Christopher A. MacRaild, Muzaffar-Ur-Rehman Mohammed, Faheem, Sankaranarayanan Murugesan, Ian K. Styles, Amanda L. Peterson, Carl M. J. Kirkpatrick, Matthew A. Cooper, Enzo A. Palombo, Moana M. Simpson, Hardik A. Jain, Vinti Agarwal, Alexander J. McAuley, Anupama Kumar, Darren J. Creek, Natalie L. Trevaskis, and Seshadri S. Vasan
- Subjects
COVID-19 ,CoviRx.org ,database ,drugs ,pandemic ,repurposing ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
SARS-CoV-2 is the cause of the COVID-19 pandemic which has claimed more than 6.5 million lives worldwide, devastating the economy and overwhelming healthcare systems globally. The development of new drug molecules and vaccines has played a critical role in managing the pandemic; however, new variants of concern still pose a significant threat as the current vaccines cannot prevent all infections. This situation calls for the collaboration of biomedical scientists and healthcare workers across the world. Repurposing approved drugs is an effective way of fast-tracking new treatments for recently emerged diseases. To this end, we have assembled and curated a database consisting of 7817 compounds from the Compounds Australia Open Drug collection. We developed a set of eight filters based on indicators of efficacy and safety that were applied sequentially to down-select drugs that showed promise for drug repurposing efforts against SARS-CoV-2. Considerable effort was made to evaluate approximately 14,000 assay data points for SARS-CoV-2 FDA/TGA-approved drugs and provide an average activity score for 3539 compounds. The filtering process identified 12 FDA-approved molecules with established safety profiles that have plausible mechanisms for treating COVID-19 disease. The methodology developed in our study provides a template for prioritising drug candidates that can be repurposed for the safe, efficacious, and cost-effective treatment of COVID-19, long COVID, or any other future disease. We present our database in an easy-to-use interactive interface (CoviRx that was also developed to enable the scientific community to access to the data of over 7000 potential drugs and to implement alternative prioritisation and down-selection strategies.
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- 2022
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19. Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection.
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Ryan J Farr, Christina L Rootes, Louise C Rowntree, Thi H O Nguyen, Luca Hensen, Lukasz Kedzierski, Allen C Cheng, Katherine Kedzierska, Gough G Au, Glenn A Marsh, Seshadri S Vasan, Chwan Hong Foo, Christopher Cowled, and Cameron R Stewart
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
The host response to SARS-CoV-2 infection provide insights into both viral pathogenesis and patient management. The host-encoded microRNA (miRNA) response to SARS-CoV-2 infection, however, remains poorly defined. Here we profiled circulating miRNAs from ten COVID-19 patients sampled longitudinally and ten age and gender matched healthy donors. We observed 55 miRNAs that were altered in COVID-19 patients during early-stage disease, with the inflammatory miR-31-5p the most strongly upregulated. Supervised machine learning analysis revealed that a three-miRNA signature (miR-423-5p, miR-23a-3p and miR-195-5p) independently classified COVID-19 cases with an accuracy of 99.9%. In a ferret COVID-19 model, the three-miRNA signature again detected SARS-CoV-2 infection with 99.7% accuracy, and distinguished SARS-CoV-2 infection from influenza A (H1N1) infection and healthy controls with 95% accuracy. Distinct miRNA profiles were also observed in COVID-19 patients requiring oxygenation. This study demonstrates that SARS-CoV-2 infection induces a robust host miRNA response that could improve COVID-19 detection and patient management.
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- 2021
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20. Live Virus Neutralisation of the 501Y.V1 and 501Y.V2 SARS-CoV-2 Variants following INO-4800 Vaccination of Ferrets
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Shane Riddell, Sarah Goldie, Alexander J. McAuley, Michael J. Kuiper, Peter A. Durr, Kim R. Blasdell, Mary Tachedjian, Julian D. Druce, Trevor R. F. Smith, Kate E. Broderick, and Seshadri S. Vasan
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biomolecular modelling ,COVID-19 ,DNA vaccine ,neutralisation ,SARS-CoV-2 antibodies ,variants ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The ongoing COVID-19 pandemic has resulted in significant global morbidity and mortality on a scale similar to the influenza pandemic of 1918. Over the course of the last few months, a number of SARS-CoV-2 variants have been identified against which vaccine-induced immune responses may be less effective. These “variants-of-concern” have garnered significant attention in the media, with discussion around their impact on the future of the pandemic and the ability of leading COVID-19 vaccines to protect against them effectively. To address concerns about emerging SARS-CoV-2 variants affecting vaccine-induced immunity, we investigated the neutralisation of representative ‘G614’, ‘501Y.V1’ and ‘501Y.V2’ virus isolates using sera from ferrets that had received prime-boost doses of the DNA vaccine, INO-4800. Neutralisation titres against G614 and 501Y.V1 were comparable, but titres against the 501Y.V2 variant were approximately 4-fold lower, similar to results reported with other nucleic acid vaccines and supported by in silico biomolecular modelling. The results confirm that the vaccine-induced neutralising antibodies generated by INO-4800 remain effective against current variants-of-concern, albeit with lower neutralisation titres against 501Y.V2 similar to other leading nucleic acid-based vaccines.
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- 2021
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21. Validation of Differentially Expressed Immune Biomarkers in Latent and Active Tuberculosis by Real-Time PCR
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Prem Perumal, Mohamed Bilal Abdullatif, Harriet N. Garlant, Isobella Honeyborne, Marc Lipman, Timothy D. McHugh, Jo Southern, Ronan Breen, George Santis, Kalaiarasan Ellappan, Saka Vinod Kumar, Harish Belgode, Ibrahim Abubakar, Sanjeev Sinha, Seshadri S. Vasan, Noyal Joseph, and Karen E. Kempsell
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tuberculosis ,biomarker ,qPCR ,validation ,diagnosis ,immune ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Tuberculosis (TB) remains a major global threat and diagnosis of active TB ((ATB) both extra-pulmonary (EPTB), pulmonary (PTB)) and latent TB (LTBI) infection remains challenging, particularly in high-burden countries which still rely heavily on conventional methods. Although molecular diagnostic methods are available, e.g., Cepheid GeneXpert, they are not universally available in all high TB burden countries. There is intense focus on immune biomarkers for use in TB diagnosis, which could provide alternative low-cost, rapid diagnostic solutions. In our previous gene expression studies, we identified peripheral blood leukocyte (PBL) mRNA biomarkers in a non-human primate TB aerosol-challenge model. Here, we describe a study to further validate select mRNA biomarkers from this prior study in new cohorts of patients and controls, as a prerequisite for further development. Whole blood mRNA was purified from ATB patients recruited in the UK and India, LTBI and two groups of controls from the UK (i) a low TB incidence region (CNTRLA) and (ii) individuals variably-domiciled in the UK and Asia ((CNTRLB), the latter TB high incidence regions). Seventy-two mRNA biomarker gene targets were analyzed by qPCR using the Roche Lightcycler 480 qPCR platform and data analyzed using GeneSpring™ 14.9 bioinformatics software. Differential expression of fifty-three biomarkers was confirmed between MTB infected, LTBI groups and controls, seventeen of which were significant using analysis of variance (ANOVA): CALCOCO2, CD52, GBP1, GBP2, GBP5, HLA-B, IFIT3, IFITM3, IRF1, LOC400759 (GBP1P1), NCF1C, PF4V1, SAMD9L, S100A11, TAF10, TAPBP, and TRIM25. These were analyzed using receiver operating characteristic (ROC) curve analysis. Single biomarkers and biomarker combinations were further assessed using simple arithmetic algorithms. Minimal combination biomarker panels were delineated for primary diagnosis of ATB (both PTB and EPTB), LTBI and identifying LTBI individuals at high risk of progression which showed good performance characteristics. These were assessed for suitability for progression against the standards for new TB diagnostic tests delineated in the published World Health Organization (WHO) technology product profiles (TPPs).
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- 2021
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22. Highly Thermotolerant SARS-CoV-2 Vaccine Elicits Neutralising Antibodies against Delta and Omicron in Mice
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Petrus Jansen van Vuren, Alexander J. McAuley, Michael J. Kuiper, Nagendrakumar Balasubramanian Singanallur, Matthew P. Bruce, Shane Riddell, Sarah Goldie, Shruthi Mangalaganesh, Simran Chahal, Trevor W. Drew, Kim R. Blasdell, Mary Tachedjian, Leon Caly, Julian D. Druce, Shahbaz Ahmed, Mohammad Suhail Khan, Sameer Kumar Malladi, Randhir Singh, Suman Pandey, Raghavan Varadarajan, and Seshadri S. Vasan
- Subjects
AlphaFold ,biomolecular modelling ,COVID-19 ,Omicron ,receptor-binding domain (RBD) ,SARS-CoV-2 ,Microbiology ,QR1-502 - Abstract
As existing vaccines fail to completely prevent COVID-19 infections or community transmission, there is an unmet need for vaccines that can better combat SARS-CoV-2 variants of concern (VOC). We previously developed highly thermo-tolerant monomeric and trimeric receptor-binding domain derivatives that can withstand 100 °C for 90 min and 37 °C for four weeks and help eliminate cold-chain requirements. We show that mice immunised with these vaccine formulations elicit high titres of antibodies that neutralise SARS-CoV-2 variants VIC31 (with Spike: D614G mutation), Delta and Omicron (BA.1.1) VOC. Compared to VIC31, there was an average 14.4-fold reduction in neutralisation against BA.1.1 for the three monomeric antigen-adjuvant combinations and a 16.5-fold reduction for the three trimeric antigen-adjuvant combinations; the corresponding values against Delta were 2.5 and 3.0. Our findings suggest that monomeric formulations are suitable for upcoming Phase I human clinical trials and that there is potential for increasing the efficacy with vaccine matching to improve the responses against emerging variants. These findings are consistent with in silico modelling and AlphaFold predictions, which show that, while oligomeric presentation can be generally beneficial, it can make important epitopes inaccessible and also carries the risk of eliciting unwanted antibodies against the oligomerisation domain.
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- 2022
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23. Euploid blastocysts implant irrespective of their morphology after NGS-(PGT-A) testing in advanced maternal age patients
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Viñals Gonzalez, X., Odia, R., Naja, R., Serhal, P., Saab, W., Seshadri, S., and Ben-Nagi, J.
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- 2019
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24. Don't Trash your Intermediate Results, Cache 'em
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Roy, Prasan, Ramamritham, Krithi, Seshadri, S., Shenoy, Pradeep, and Sudarshan, S.
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Computer Science - Databases ,H.2.4 ,H.2.7 - Abstract
In data warehouse and data mart systems, queries often take a long time to execute due to their complex nature. Query response times can be greatly improved by caching final/intermediate results of previous queries, and using them to answer later queries. In this paper we describe a caching system called Exchequer which incorporates several novel features including optimization aware cache maintenance and the use of a cache aware optimizer. In contrast, in existing work, the module that makes cost-benefit decisions is part of the cache manager and works independent of the optimizer which essentially reconsiders these decisions while finding the best plan for a query. In our work, the optimizer takes the decisions for the cache manager. Furthermore, existing approaches are either restricted to cube (slice/point) queries, or cache just the query results. On the other hand, our work is extens ible and in fact presents a data-model independent framework and algorithm. Our experimental results attest to the efficacy of our cache management techniques and show that over a wide range of parameters (a) Exchequer's query response times are lower by more than 30% compared to the best performing competitor, and (b) Exchequer can deliver the same response time as its competitor with just one tenth of the cache size., Comment: 22 pages, 4 figures
- Published
- 2000
25. Quantum revivals, geometric phases and circle map recurrences
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Seshadri, S., Lakshmibala, S., and Balakrishnan, V.
- Subjects
Quantum Physics ,Nonlinear Sciences - Chaotic Dynamics - Abstract
Revivals of the coherent states of a deformed, adiabatically and cyclically varying oscillator Hamiltonian are examined. The revival time distribution is exactly that of Poincar\'{e} recurrences for a rotation map: only three distinct revival times can occur, with specified weights. A link is thus established between quantum revivals and recurrences in a coarse-grained discrete-time dynamical system., Comment: 9 pages
- Published
- 1999
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26. Efficient and Extensible Algorithms for Multi Query Optimization
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Roy, Prasan, Seshadri, S., Sudarshan, S., and Bhobe, Siddhesh
- Subjects
Computer Science - Databases ,H.2.4 ,H.2.7 - Abstract
Complex queries are becoming commonplace, with the growing use of decision support systems. These complex queries often have a lot of common sub-expressions, either within a single query, or across multiple such queries run as a batch. Multi-query optimization aims at exploiting common sub-expressions to reduce evaluation cost. Multi-query optimization has hither-to been viewed as impractical, since earlier algorithms were exhaustive, and explore a doubly exponential search space. In this paper we demonstrate that multi-query optimization using heuristics is practical, and provides significant benefits. We propose three cost-based heuristic algorithms: Volcano-SH and Volcano-RU, which are based on simple modifications to the Volcano search strategy, and a greedy heuristic. Our greedy heuristic incorporates novel optimizations that improve efficiency greatly. Our algorithms are designed to be easily added to existing optimizers. We present a performance study comparing the algorithms, using workloads consisting of queries from the TPC-D benchmark. The study shows that our algorithms provide significant benefits over traditional optimization, at a very acceptable overhead in optimization time.
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- 1999
27. Control of Wave Packet Revivals Using Geometric Phases
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Seshadri, S., Lakshmibala, S., and Balakrishnan, V.
- Subjects
Quantum Physics ,Nonlinear Sciences - Chaotic Dynamics - Abstract
Wave packets in a system governed by a Hamiltonian with a generic nonlinear spectrum typically exhibit both full and fractional revivals. It is shown that the latter can be eliminated by inducing suitable geometric phases in the states, by varying the parameters in the Hamiltonian cyclically with a period T. Further, with the introduction of this natural time step T, the occurrence of near revivals can be mapped onto that of Poincar\'{e} recurrences in an irrational rotation map of the circle. The distinctive recurrence time statistics of the latter can thus serve as a clear signature of the dynamics of wave packet revivals., Comment: 11 pages
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- 1999
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28. Ladder operators for isospectral oscillators
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Seshadri, S., Balakrishnan, V., and Lakshmibala, S.
- Subjects
Quantum Physics - Abstract
We present, for the isospectral family of oscillator Hamiltonians, a systematic procedure for constructing raising and lowering operators satisfying any prescribed `distorted' Heisenberg algebra (including the $q$-generalization). This is done by means of an operator transformation implemented by a shift operator. The latter is obtained by solving an appropriate partial isometry condition in the Hilbert space. Formal representations of the non-local operators concerned are given in terms of pseudo-differential operators. Using the new annihilation operators, new classes of coherent states are constructed for isospectral oscillator Hamiltonians. The corresponding Fock-Bargmann representations are also considered, with specific reference to the order of the entire function family in each case., Comment: 13 pages
- Published
- 1999
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29. Geometric phases for generalized squeezed coherent states
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Seshadri, S., Lakshmibala, S., and Balakrishnan, V.
- Subjects
Quantum Physics - Abstract
A simple technique is used to obtain a general formula for the Berry phase (and the corresponding Hannay angle) for an arbitrary Hamiltonian with an equally-spaced spectrum and appropriate ladder operators connecting the eigenstates. The formalism is first applied to a general deformation of the oscillator involving both squeezing and displacement. Earlier results are shown to emerge as special cases. The analysis is then extended to multiphoton squeezed coherent states and the corresponding anholonomies deduced., Comment: 15 pages
- Published
- 1999
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30. Noise-amplitude dependence of the invariant density for noisy, fully chaotic one-dimensional maps
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Seshadri, S., Balakrishnan, V., and Lakshmibala, S.
- Subjects
Nonlinear Sciences - Chaotic Dynamics - Abstract
We present some analytic, non-perturbative results for the invariant density rho(x) for noisy one-dimensional maps at fully developed chaos. Under periodic boundary conditions, the Fourier expansion method is used to show precisely how noise makes rho(x) absolutely continuous and smoothens it out. Simple solvable models are used to illustrate the explicit dependence of rho(x) on the amplitude eta of the noise distribution, all the way from the case of zero noise (eta > 0) to the completely noise-dominated limit (eta=1)., Comment: 15 pages, 5 Postscript figures (To appear in Phys. Rev. E)
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- 1999
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31. Development of NIR detectors and science driven requirements for SNAP
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Brown, M.G., Bebek, C., Bernstein, G., Bonissent, A., Carithers, B., Cole, D., Figer, D., Gerdes, D., Gladney, L., Lorenzon, W., Kim, A., Kushner, G., Kuznetsova, N., Linder, E., McKee, S., Miquel, R., Mostek, N., Mufson, S., Schubnell, M., Seshadri, S., Shukla, H., Smith, R., Stebbins, A., Stoughton, C., and Tarle, G.
- Subjects
Instrumentation -- other ,NIR detectors HgCdTe supernova dark energy SNAP - Abstract
Precision near infrared (NIR) measurements are essential for the next generation of ground and space based instruments. The SuperNova Acceleration Probe (SNAP) will measure thousands of type Ia supernovae up to a redshift of 1.7. The highest redshift supernovae provide the most leverage for determining cosmological parameters, in particular the dark energy equation of state and its possible time evolution. Accurate NIR observations are needed to utilize the full potential of the highest redshift supernovae. Technological improvements in NIR detector fabrication have lead to high quantum efficiency, low noise detectors using a HgCdTe diode with a band-gap that is tuned to cutoff at 1:7 1m. The effects of detector quantum efficiency, read noise, and dark current on lightcurve signal to noise, lightcurve parameter errors, and distance modulus ?ts are simulated in the SNAP sim framework. Results show that improving quantum efficiency leads to the largest gains in photometric accuracy for type Ia supernovae. High quantum efficiency in the NIR reduces statistical errors and helps control systematic uncertainties at the levels necessary to achieve the primary SNAP science goals.
- Published
- 2006
32. Characterization of NIR InGaAs imager arrays for the JDEM SNAP mission concept
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Seshadri, S., Cole, M.D., Hancock, B., Ringold, P., Wrigley, C., Bonati, M., Brown, M.G., Schubnell, M., Rahmer, G., Guzman, D., Figer, D., Tarle, G., Smith, R.M., and Bebek, C.
- Subjects
Instrumentation -- other ,NIR InGaAs astronomy low-temperature focal plane array imager SNAP - Abstract
We present the results of a study of the performance of InGaAs detectors conducted for the SuperNova Acceleration Probe (SNAP) dark energy mission concept. Low temperature data from a nominal 1.7um cut-off wavelength 1kx1k InGaAs photodiode array, hybridized to a Rockwell H1RG multiplexer suggest that InGaAs detector performance is comparable to those of existing 1.7um cut-off HgCdTe arrays. Advances in 1.7um HgCdTe dark current and noise initiated by the SNAP detector research and development program makes it the baseline detector technology for SNAP. However, the results presented herein suggest that existing InGaAs technology is a suitable alternative for other future astronomy applications.
- Published
- 2006
33. Near infrared detectors for SNAP
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Schubnell, M., Barron, N., Bebek, C., Brown, M.G., Borysow, M., Cole, D., Figer, D., Lorenzon, W., Mostek, N., Mufson, S., Seshadri, S., Smith, R., and Tarle, G.
- Subjects
Instrumentation -- other ,Near Infrared HgCdTe Detector Technology Photometry Dark Energy Cosmology Supernovae - Abstract
Large format (1k x 1k and 2k x 2k) near infrared detectors manufactured by Rockwell Scientific Center and Raytheon Vision Systems are characterized as part of the near infrared R&D effort for SNAP (the Super-Nova/Acceleration Probe). These are hybridized HgCdTe focal plane arrays with a sharp high wavelength cut-off at 1.7 um. This cut-off provides a sufficiently deep reach in redshift while it allows at the same time low dark current operation of the passively cooled detectors at 140 K. Here the baseline SNAP near infrared system is briefly described and the science driven requirements for the near infrared detectors are summarized. A few results obtained during the testing of engineering grade near infrared devices procured for the SNAP project are highlighted. In particular some recent measurements that target correlated noise between adjacent detector pixels due to capacitive coupling and the response uniformity within individual detector pixels are discussed.
- Published
- 2006
34. Metabolic Profiling from an Asymptomatic Ferret Model of SARS-CoV-2 Infection
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David J. Beale, Rohan Shah, Avinash V. Karpe, Katie E. Hillyer, Alexander J. McAuley, Gough G. Au, Glenn A. Marsh, and Seshadri S. Vasan
- Subjects
animal model ,COVID-19 ,ferret ,lipidomics ,metabolomics ,SARS-CoV-2 ,Microbiology ,QR1-502 - Abstract
Coronavirus disease (COVID-19) is a contagious respiratory disease that is causing significant global morbidity and mortality. Understanding the impact of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection on the host metabolism is still in its infancy but of great importance. Herein, we investigated the metabolic response during viral shedding and post-shedding in an asymptomatic SARS-CoV-2 ferret model (n = 6) challenged with two SARS-CoV-2 isolates. Virological and metabolic analyses were performed on (minimally invasive) collected oral swabs, rectal swabs, and nasal washes. Fragments of SARS-CoV-2 RNA were only found in the nasal wash samples in four of the six ferrets, and in the samples collected 3 to 9 days post-infection (referred to as viral shedding). Central carbon metabolism metabolites were analyzed during viral shedding and post-shedding periods using a dynamic Multiple Reaction Monitoring (dMRM) database and method. Subsequent untargeted metabolomics and lipidomics of the same samples were performed using a Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LC-QToF-MS) methodology, building upon the identified differentiated central carbon metabolism metabolites. Multivariate analysis of the acquired data identified 29 significant metabolites and three lipids that were subjected to pathway enrichment and impact analysis. The presence of viral shedding coincided with the challenge dose administered and significant changes in the citric acid cycle, purine metabolism, and pentose phosphate pathways, amongst others, in the host nasal wash samples. An elevated immune response in the host was also observed between the two isolates studied. These results support other metabolomic-based findings in clinical observational studies and indicate the utility of metabolomics applied to ferrets for further COVID-19 research that advances early diagnosis of asymptomatic and mild clinical COVID-19 infections, in addition to assessing the effectiveness of new or repurposed drug therapies.
- Published
- 2021
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35. Structure and Corrosion of High Voltage Anodic Oxide Coatings on Ti6Al4V Biomaterial
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Narayanan, R. and Seshadri, S. K.
- Published
- 2018
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36. Production, characterization and evaluation of biochar from pod (Pisum sativum), leaf (Brassica oleracea)and peel (Citrus sinensis)wastes
- Author
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Stella Mary, G., Sugumaran, P., Niveditha, S., Ramalakshmi, B., Ravichandran, P., and Seshadri, S.
- Abstract
Biochar has potential as a valuable tool for the agricultural industry with its unique ability to help build soil health, increase physical properties of soil, soil pH, organic carbon content, conserve water and mitigate drought, reduce GHG emission, conserve nutrients, decrease fertilizer requirements, sequester carbon, increase crop productivity and serve as a most preferred habitat for microbes. In this study, three perishable biomass wastes viz. Pea pod (Pisum sativum), cauliflower leaves (Brassica oleracea) and orange peel wastes (Citrus sinensis) were carbonized and characterized for differential application. The biomass was subjected to carbonization at different temperatures from 100 to 600 °C for 1 h. Biomass and biochar samples were characterized for proximate (M, VM, FC, Ash), ultimate (CHNS-O), biochemical properties (Ce, He, Li), thermo gravimetric analysis, pH, EC and bulk density. The biochars were also analyzed through SEM and FTIR for identification of pore size and functional groups. The char yield was high in cauliflower leaf (30.16 %), followed by orange peel (25.54 %) and pea pod (21.154 %) at 300 °C. The total organic carbon (11.61 %), total negative surface anions (4.25 mmol H+eq/g C) and water holding capacity (200 %) were high in pea pod biochar. The SEM images of biochar samples showed plane cleavage surfaces with broken edges. The surface functional groups of all the three biochar samples were hydroxyl, methyl, carboxylic and alkene groups. The pea pod and cauliflower leaf biochar showed higher values of organic carbon, total surface anions, water holding capacity and mineral content and performed as best soil amendment than orange peel biochar. These biochar can be used as an effective medium for increasing soil carbon, irrigation efficiency and efficient disposal of agricultural waste-biomass.
- Published
- 2024
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37. Performance Evaluation of Mass Storage Systems for Scientific Databases
- Author
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Segev, A., Seshadri, S., and Rotem, D.
- Published
- 1994
38. Effective C–N–P ratio for growth and nutrient removal efficiency of Scenedesmus obliquus in sugar mill effluent
- Author
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Shashirekha, V., Sivakumar, M., and Seshadri, S.
- Published
- 2016
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39. Production, characterization and evaluation of biochar from pod (Pisum sativum), leaf (Brassica oleracea) and peel (Citrus sinensis) wastes
- Author
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Stella Mary, G., Sugumaran, P., Niveditha, S., Ramalakshmi, B., Ravichandran, P., and Seshadri, S.
- Published
- 2016
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40. GWAS for executive function and processing speed suggests involvement of the CADM2 gene
- Author
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Ibrahim-Verbaas, C A, Bressler, J, Debette, S, Schuur, M, Smith, A V, Bis, J C, Davies, G, Trompet, S, Smith, J A, Wolf, C, Chibnik, L B, Liu, Y, Vitart, V, Kirin, M, Petrovic, K, Polasek, O, Zgaga, L, Fawns-Ritchie, C, Hoffmann, P, Karjalainen, J, Lahti, J, Llewellyn, D J, Schmidt, C O, Mather, K A, Chouraki, V, Sun, Q, Resnick, S M, Rose, L M, Oldmeadow, C, Stewart, M, Smith, B H, Gudnason, V, Yang, Q, Mirza, S S, Jukema, J W, deJager, P L, Harris, T B, Liewald, D C, Amin, N, Coker, L H, Stegle, O, Lopez, O L, Schmidt, R, Teumer, A, Ford, I, Karbalai, N, Becker, J T, Jonsdottir, M K, Au, R, Fehrmann, R S N, Herms, S, Nalls, M, Zhao, W, Turner, S T, Yaffe, K, Lohman, K, van Swieten, J C, Kardia, S L R, Knopman, D S, Meeks, W M, Heiss, G, Holliday, E G, Schofield, P W, Tanaka, T, Stott, D J, Wang, J, Ridker, P, Gow, A J, Pattie, A, Starr, J M, Hocking, L J, Armstrong, N J, McLachlan, S, Shulman, J M, Pilling, L C, Eiriksdottir, G, Scott, R J, Kochan, N A, Palotie, A, Hsieh, Y-C, Eriksson, J G, Penman, A, Gottesman, R F, Oostra, B A, Yu, L, DeStefano, A L, Beiser, A, Garcia, M, Rotter, J I, Nöthen, M M, Hofman, A, Slagboom, P E, Westendorp, R G J, Buckley, B M, Wolf, P A, Uitterlinden, A G, Psaty, B M, Grabe, H J, Bandinelli, S, Chasman, D I, Grodstein, F, Räikkönen, K, Lambert, J-C, Porteous, D J, Price, J F, Sachdev, P S, Ferrucci, L, Attia, J R, Rudan, I, Hayward, C, Wright, A F, Wilson, J F, Cichon, S, Franke, L, Schmidt, H, Ding, J, de Craen, A J M, Fornage, M, Bennett, D A, Deary, I J, Ikram, M A, Launer, L J, Fitzpatrick, A L, Seshadri, S, van Duijn, C M, and Mosley, T H
- Published
- 2016
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41. A novel Alzheimer disease locus located near the gene encoding tau protein
- Author
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Jun, G, Ibrahim-Verbaas, C A, Vronskaya, M, Lambert, J-C, Chung, J, Naj, A C, Kunkle, B W, Wang, L-S, Bis, J C, Bellenguez, C, Harold, D, Lunetta, K L, Destefano, A L, Grenier-Boley, B, Sims, R, Beecham, G W, Smith, A V, Chouraki, V, Hamilton-Nelson, K L, Ikram, M A, Fievet, N, Denning, N, Martin, E R, Schmidt, H, Kamatani, Y, Dunstan, M L, Valladares, O, Laza, A R, Zelenika, D, Ramirez, A, Foroud, T M, Choi, S-H, Boland, A, Becker, T, Kukull, W A, van der Lee, S J, Pasquier, F, Cruchaga, C, Beekly, D, Fitzpatrick, A L, Hanon, O, Gill, M, Barber, R, Gudnason, V, Campion, D, Love, S, Bennett, D A, Amin, N, Berr, C, Tsolaki, Magda, Buxbaum, J D, Lopez, O L, Deramecourt, V, Fox, N C, Cantwell, L B, Tárraga, L, Dufouil, C, Hardy, J, Crane, P K, Eiriksdottir, G, Hannequin, D, Clarke, R, Evans, D, Mosley, Jr., T H, Letenneur, L, Brayne, C, Maier, W, De Jager, P, Emilsson, V, Dartigues, J-F, Hampel, H, Kamboh, M I, de Bruijn, R F A G, Tzourio, C, Pastor, P, Larson, E B, Rotter, J I, O'Donovan, M C, Montine, T J, Nalls, M A, Mead, S, Reiman, E M, Jonsson, P V, Holmes, C, St George-Hyslop, P H, Boada, M, Passmore, P, Wendland, J R, Schmidt, R, Morgan, K, Winslow, A R, Powell, J F, Carasquillo, M, Younkin, S G, Jakobsdóttir, J, Kauwe, J S K, Wilhelmsen, K C, Rujescu, D, Nöthen, M M, Hofman, A, Jones, L, Haines, J L, Psaty, B M, Van Broeckhoven, C, Holmans, P, Launer, L J, Mayeux, R, Lathrop, M, Goate, A M, Escott-Price, V, Seshadri, S, Pericak-Vance, M A, Amouyel, P, Williams, J, van Duijn, C M, Schellenberg, G D, and Farrer, L A
- Published
- 2016
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42. Downy Brome (Bromus tectorum L.) and Broadleaf Weed Control in Winter Wheat with Acetolactate Synthase-Inhibiting Herbicides
- Author
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Patrick W. Geier, Phillip W. Stahlman, and Seshadri S. Reddy
- Subjects
blue mustard ,downy brome ,flixweed ,henbit ,propoxycarbazone-Na ,pyroxsulam ,sulfosulfuron ,Agriculture - Abstract
A study was conducted for three seasons in northwest Kansas, USA to evaluate acetolactate synthase (ALS)-inhibiting herbicides for downy brome (Bromus tectorum L.) and winter annual broadleaf weed control in winter wheat. Herbicides included pyroxsulam at 18.4 g ai ha−1, propoxycarbazone-Na at 44 g ai ha−1, premixed propoxycarbazone-Na & mesosulfuron-methyl at 27 g ai ha−1, and sulfosulfuron at 35 g ai ha−1. The herbicides were applied postemergence in fall and spring seasons. Averaged over time of application, no herbicide controlled downy brome more than 78% in any year. When downy brome densities were high, control was less than 60%. Pyroxsulam controlled downy brome greater than or similar to other herbicides tested. Flixweed (Descurainia sophia L.), blue mustard [Chorispora tenella (Pallas) DC.], and henbit (Lamium amplexicaule L.) control did not differ among herbicide treatments. All herbicides tested controlled flixweed and blue mustard at least 87% and 94%, respectively. However, none of the herbicides controlled henbit more than 73%. Fall herbicide applications improved weed control compared to early spring applications; improvement ranged from 3% to 31% depending on the weed species. Henbit control was greatly decreased by delaying herbicide applications until spring compared to fall applications (49% vs. 80% control). Herbicide injury was observed in only two instances. The injury was ≤13% with no difference between herbicides and the injury did not impact final plant height or grain yield.
- Published
- 2013
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43. DISC1 regulates trafficking and processing of APP and Aβ generation
- Author
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Shahani, N, Seshadri, S, Jaaro-Peled, H, Ishizuka, K, Hirota-Tsuyada, Y, Wang, Q, Koga, M, Sedlak, T W, Korth, C, Brandon, N J, Kamiya, A, Subramaniam, S, Tomoda, T, and Sawa, A
- Published
- 2015
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- View/download PDF
44. Genetic contributions to variation in general cognitive function: a meta-analysis of genome-wide association studies in the CHARGE consortium (N=53 949)
- Author
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Davies, G, Armstrong, N, Bis, J C, Bressler, J, Chouraki, V, Giddaluru, S, Hofer, E, Ibrahim-Verbaas, C A, Kirin, M, Lahti, J, van der Lee, S J, Le Hellard, S, Liu, T, Marioni, R E, Oldmeadow, C, Postmus, I, Smith, A V, Smith, J A, Thalamuthu, A, Thomson, R, Vitart, V, Wang, J, Yu, L, Zgaga, L, Zhao, W, Boxall, R, Harris, S E, Hill, W D, Liewald, D C, Luciano, M, Adams, H, Ames, D, Amin, N, Amouyel, P, Assareh, A A, Au, R, Becker, J T, Beiser, A, Berr, C, Bertram, L, Boerwinkle, E, Buckley, B M, Campbell, H, Corley, J, De Jager, P L, Dufouil, C, Eriksson, J G, Espeseth, T, Faul, J D, Ford, I, Scotland, Generation, Gottesman, R F, Griswold, M E, Gudnason, V, Harris, T B, Heiss, G, Hofman, A, Holliday, E G, Huffman, J, Kardia, S L R, Kochan, N, Knopman, D S, Kwok, J B, Lambert, J-C, Lee, T, Li, G, Li, S-C, Loitfelder, M, Lopez, O L, Lundervold, A J, Lundqvist, A, Mather, K A, Mirza, S S, Nyberg, L, Oostra, B A, Palotie, A, Papenberg, G, Pattie, A, Petrovic, K, Polasek, O, Psaty, B M, Redmond, P, Reppermund, S, Rotter, J I, Schmidt, H, Schuur, M, Schofield, P W, Scott, R J, Steen, V M, Stott, D J, van Swieten, J C, Taylor, K D, Trollor, J, Trompet, S, Uitterlinden, A G, Weinstein, G, Widen, E, Windham, B G, Jukema, J W, Wright, A F, Wright, M J, Yang, Q, Amieva, H, Attia, J R, Bennett, D A, Brodaty, H, de Craen, A J M, Hayward, C, Ikram, M A, Lindenberger, U, Nilsson, L-G, Porteous, D J, Räikkönen, K, Reinvang, I, Rudan, I, Sachdev, P S, Schmidt, R, Schofield, P R, Srikanth, V, Starr, J M, Turner, S T, Weir, D R, Wilson, J F, van Duijn, C, Launer, L, Fitzpatrick, A L, Seshadri, S, Mosley, Jr, T H, and Deary, I J
- Published
- 2015
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45. First report on ALS herbicide resistance in barnyardgrass (Echinochloa crus-galli) from rice fields of India
- Author
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Choudhary, Vijay K., Reddy, Seshadri S., Mishra, Subhash K., Gharde, Yogita, Kumar, Sunil, Yadav, Mayank, Barik, Suhrid, and Singh, P.K.
- Abstract
AbstractBispyribac-sodium, a herbicide that inhibits acetolactate synthase (ALS), is frequently used in rice fields in India to control weeds, including the most common noxious weed, barnyardgrass. However, rice growers have recently reported reduced control of barnyardgrass with bispyribac-sodium. Hence, a large-scale survey was carried out to assess bispyribac-sodium resistance in Chhattisgarh and Kerala, two rice-growing states. Open-field pot experiments were conducted for 2 yr to confirm resistance to bispyribac-sodium. Of the 37 biotypes tested, 30% (11) survived the recommended label rate of bispyribac-sodium (25 g ai ha−1). The effective rate of bispyribac-sodium required to achieve 50% control (ED50) of putative resistant biotypes ranged from 18 to 41 g ha−1, whereas it was about 10 g ha−1for susceptible biotypes. This suggests that putative biotypes were two to four times more resistant to bispyribac-sodium. At 6 d after herbicide application, an in vitro enzyme assay demonstrated higher ALS enzyme activity in putative resistant biotypes (66% to 75%) compared with susceptible biotypes (48% to 52%). This indicates the presence of an insensitive ALS enzyme in those biotypes and a target site mutation as a possible mechanism for resistance. Whole-plant bioassays also suggested that the resistance problem is more widespread in Chhattisgarh than in Kerala. This study confirmed the first case of evolved resistance in barnyardgrass to bispyribac-sodium in rice fields of India.
- Published
- 2023
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46. Relationship Between Cotton Varieties and Moisture Vapor Transport of Knitted Fabrics
- Author
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Seshadri S. Ramkumar, Ph.D, Arvind Purushothaman, Kater D. Hake, Ph.D., and David D. McAlister, III
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Chemical technology ,TP1-1185 ,Textile bleaching, dyeing, printing, etc. ,TP890-933 - Abstract
Cotton offers next-to-skin comfort and hence is a preferred fiber for undergarments. There have been a number of studies on the effect of different fiber types, fabric structure, fabric finishes, etc. on the moisture vapor transport properties. However, there has been no report in the public domain on the relationship between cotton varieties on the moisture vapor transport characteristics of fabrics produced from them. The study reported in this paper focuses on the moisture vapor transport properties (MVTR) of cotton fabrics knitted from 31 different cotton fibers with different pedigrees grown over a three-year period in three major cotton growing regions of US, Southwest (Texas), Mid-South (Mississippi) and Southeast (Georgia). Results indicate that cotton varieties influence the MVTR of knitted fabrics produced from them. This study, for the first time has attempted to link varietal effects on the most important property of cotton—breathability quantified using MVTR. Preliminary analysis indicates a relationship between the basic sugar content such as verbascose of cotton and its moisture vapor transport. However this result has to be followed up with a thorough study.
- Published
- 2007
47. Influence of carbon-dioxide on the growth of Spirulina sp. (MCRC-A0003) isolated from Muttukadu backwaters, South India
- Author
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Sivakumar, M., Ranjith Kumar, R., Shashirekha, V., and Seshadri, S.
- Published
- 2014
- Full Text
- View/download PDF
48. Allelic differences between Europeans and Chinese for CREB1 SNPs and their implications in gene expression regulation, hippocampal structure and function, and bipolar disorder susceptibility
- Author
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Li, M, Luo, X-j, Rietschel, M, Lewis, C M, Mattheisen, M, Müller-Myhsok, B, Jamain, S, Leboyer, M, Landén, M, Thompson, P M, Cichon, S, Nöthen, M M, Schulze, T G, Sullivan, P F, Bergen, S E, Donohoe, G, Morris, D W, Hargreaves, A, Gill, M, Corvin, A, Hultman, C, Toga, A W, Shi, L, Lin, Q, Shi, H, Gan, L, Meyer-Lindenberg, A, Czamara, D, Henry, C, Etain, B, Bis, J C, Ikram, M A, Fornage, M, Debette, S, Launer, L J, Seshadri, S, Erk, S, Walter, H, Heinz, A, Bellivier, F, Stein, J L, Medland, S E, Arias Vasquez, A, Hibar, D P, Franke, B, Martin, N G, Wright, M J, and Su, B
- Published
- 2014
- Full Text
- View/download PDF
49. Determination of Chorionicity in Twins: Feasibility at a Tertiary Care Centre and Audit of Current Practice in the Community
- Author
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Pilli, M. L., Balusamy, S. L., Ramesh, J., Suresh, I., Seshadri, S., and Sairam, S.
- Published
- 2014
- Full Text
- View/download PDF
50. Defect-sealing synthesis of vertically oriented ordered mesoporous silica membranes
- Author
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Seshadri, S. K. and Lin, Y. S.
- Published
- 2014
- Full Text
- View/download PDF
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