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This study examines the impact of access to India’s farm science centers (Krishi Vigyan Kendras, or KVKs) on agricultural households’ welfare using household data from the nationally representative Situation Assessment Survey of Agricultural Households conducted by India’s National Sample Survey Office in 2013. Employing different matching techniques and endogenous switching regression models, it was observed that the KVKs have a positive and statistically significant impact on agricultural households’ economic welfare, although, that impact is heterogeneous. Further, the investments made in expansion of India’s network of KVKs have been quite remunerative, as the benefit- to-cost ratio of expenditure on KVKs ranges from 8–12. Moreover, present findings suggest that expanding rural formal credit markets and promoting literacy can maximize the potential impact of KVKs on agricultural households’ economic welfare.

Surface irrigation methods in puddle transplanted rice (PTR) requires a huge amount of irrigation water and leads to decline in water table in rice (Oryza sativa L.) growing areas of Indo-Gangetic Plains (IGP) of India. Mini sprinkler irrigation system with high water application efficiency in direct seeded rice (DSR) can save substantial amount of irrigation water. With this hypothesis, a four-year field experiment was conducted at ICAR-Central Soil Salinity Research Institute, Karnal to assess the feasibility of mini-sprinkler irrigation system for growing rice crop under conservation agriculture. Total 4 treatments, viz. mini-sprinkler irrigation system in direct seeded rice (SPRL-DSR), mini-sprinkler irrigation system in direct seeded rice with 1/3rd wheat residue (SPRL-DSR+R), surface irrigation in direct seeded rice (SUR-DSR), and surface irrigation in puddle transplanted rice (SUR-TPR), were laid out. Significantly higher grain yield, grains/panicle, and 1000-grain weight were recorded under SUR-TPR, while higher number of effective tillers/m.r.l. was recorded in SUR-DSR. The yield penalty in SPRL-DSR (6.84 t/ha) was 8.3% as compared to SUR-TPR. The saving in irrigation water and nitrogen was found to be 52.8 and 26.7%, respectively in SPRL-DSR as compared to SUR-TPR. SPRL-DSR also recorded 1.6-2.6 times higher grain water productivity (GWP) in comparison of SUR-TPR. Higher water and nitrogen use efficiency suggest that mini-sprinkler irrigation system is a viable option for DSR cultivation in groundwater depleting Indo-Gangetic plains of India.

Abstract Background Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery. Results To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N = 1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3–5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism. Conclusions Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.

Abstract Space-based research can provide a major leap forward in the study of key open questions in the fundamental physics domain. They include the validity of Einstein’s Equivalence principle, the origin and the nature of dark matter and dark energy, decoherence and collapse models in quantum mechanics, and the physics of quantum many-body systems. Cold-atom sensors and quantum technologies have drastically changed the approach to precision measurements. Atomic clocks and atom interferometers as well as classical and quantum links can be used to measure tiny variations of the space-time metric, elusive accelerations, and faint forces to test our knowledge of the physical laws ruling the Universe. In space, such instruments can benefit from unique conditions that allow improving both their precision and the signal to be measured. In this paper, we discuss the scientific priorities of a space-based research program in fundamental physics.

A large-scale GWAS provides insight on diabetes-dependent genetic effects on the glomerular filtration rate, a common metric to monitor kidney health in disease.

Abstract Wild bees are important pollinators and monitoring their abundance and diversity is necessary to develop conservation protocols. It is imperative to understand differences in sampling efficiency among different trap types to help guide monitoring efforts. This study used a new vane trap design to collect bees in a livestock pasture ecosystem and examined the impact of six different vane colors on wild bee sampling. We recorded 2230 bees comprising 49 species and five families. The most abundant species were Augochlorella aurata (25.8%), Lasioglossum disparile (18.3%), Lasioglossum imitatum (10.85%), Agapostemon texanus (10.8%), Melissodes vernoniae (9.9%) and Halictus ligatus (4.7%). Traps with bright blue vanes captured the greatest number and diversity of bees as compared to traps with bright yellow, dark blue, dark yellow, and purple vanes. Red vanes had the lowest captures rates of individuals and species. Different colors were associated with different bee species arrays and only nine species were found in all vane color types. Vanes with higher light reflectance properties (within 400–600 nm range) attracted the greatest number of bees. These results show that different light wavelengths and reflectivity of vane traps influence bee capture rates, and such findings can help optimize bee sampling methods in different ecosystems.

Genome‐wide association studies (GWAS) have identified several risk loci for nonalcoholic fatty liver disease (NAFLD). Previous studies have largely relied on small sample sizes and have assessed quantitative traits. We performed a case‐control GWAS in the UK Biobank using recorded diagnosis of NAFLD based on diagnostic codes recommended in recent consensus guidelines. We performed a GWAS of 4,761 cases of NAFLD and 373,227 healthy controls without evidence of NAFLD. Sensitivity analyses were performed excluding other co‐existing hepatic pathology, adjusting for body mass index (BMI) and adjusting for alcohol intake. A total of 9,723,654 variants were assessed by logistic regression adjusted for age, sex, genetic principal components, and genotyping batch. We performed a GWAS meta‐analysis using available summary association statistics. Six risk loci were identified (P

A 6-month-old boy, a case of Shone's complex, presented in decompensated state was found to have severe mitral stenosis along with multisystem inflammatory syndrome in children (MISC) warranting urgent surgical intervention. Various modalities including cytokine-adsorbing hemofilter were used to target inflammation. Postoperatively, the child recovered from low cardiac output accompanied by decrease in the levels of inflammatory markers, inopressors, and ventilatory requirements. Open heart surgery in itself is a proinflammatory process and is best avoided during the active inflammatory phase of MISC. In the rare and unavoidable circumstance exemplified by this index case, multipronged strategy targeting inflammation as described can be successfully implemented.

Background : Sternal clefts are rare congenital anterior chest wall defects created by a lack of midline thoracic fusion. Various surgical repairs have been proposed to provide protection to underlying viscera in these defects. Aim : This study aims to perform primary sternal cleft repair using techniques, leading to the provision of a complete bony cover and to assess their outcomes on follow-ups. Materials and Methods : During 2009–2020, seven patients were referred to our unit with sternal defects. Out of them, four infants with sternal clefts underwent primary repair using bilateral perichondrial flap creation of the sternal bars and sliding costal chondrotomy at our institute. In one of them with a wider defect, bilateral “intraperiosteal” sliding clavicular osteotomy was additionally performed to achieve tension-free closure. Results : Satisfactory surgical outcomes were achieved with an uneventful postoperative period. On follow-up, all four patients are thriving well and have a stable anterior chest wall. Those with follow-ups longer than 5 years showed evidence of bone formation. Conclusion : Bony cover to the heart can be provided in all varieties of sternal cleft defects using primary surgical repair early in infancy. The delay in surgical correction increases the complexity of the procedure and may require the use of prosthetic material which has its own disadvantages.

Purpose: To analyze the effect of various macular hole indices and postoperative microstructural changes of all retinal layers on postoperative functional outcomes in patients with idiopathic full-thickness macular hole (FTMH). Methods: In this prospective study, pre and post-operative optical coherence tomography (OCT) scans of 36 eyes with idiopathic FTMH were analyzed. Hole indices and microstructural changes of all retinal layers such as ellipsoid zone (EZ), external limiting membrane (ELM) integrity, outer and inner retinal defects, and cystoid resolution were studied on follow-up visits. Results: Out of 36 eyes, type-1 closure was achieved in 23 eyes (65.7%) and type-2 closure in 11 eyes (31.42%), one eye showed persistent hole, and one eye was lost to follow-up. The mean minimum diameter of hole (P = 0.026), mean MHI (P = 0.001), DHI (P = 0.158), THI (P = 0.001), and HFF (P < 0.001) showed statistical significance with the type of hole closure. Postoperatively, eyes with intact ELM and EZ had better BCVA at the final visit. The BCVA was better by logMAR 0.73 ± 0.38 (P < 0.001) in patients with absent outer retinal defects. There was a significant difference in BCVA of 0.52 ± 0.35 at 1 month and 0.64 ± 0.34 at 6 months in eyes without inner retinal defects (P < 0.001). At 6 months, cystoid resolution was observed in 28 (80%) eyes. BCVA was significantly better at 1 month (P < 0.001) and at 6 months (P = 0.001) in eyes with no DONFL. Conclusion: Macular hole indices determine the closure type. Postoperative regeneration of outer retinal layers and resolution of retinal defects significantly influence the final visual outcomes. ELM recovery is seen as a prerequisite for EZ regeneration with no new IRD after a period of 3 months.

Background: E-aushadhi is a drug supply chain management initiative of the Rajasthan government. This study is conducted to assess this e-health program as evidence is lacking in this context. Methods: A mix-method study was conducted. Primary data were recorded from key stakeholders using qualitative interviews. Secondary data were collected from internet-based searches, reports, documents, and available literature. Findings were contextualized into the Benefit-Evaluation framework using six dimensions. Results: E-aushadhi provides a systematic approach for sourcing, storing, and re-distribution of essential medicine through its three-tier structure. Its user-friendly dashboard entails accurate entries, customizable reports, and easy tracking. It has reduced workload and improved information management with timely drug supply while allowing monitoring with key performance indicators. Conclusions: E-aushadhi has been successful in improving beneficiary access at public health facilities and may act as a backbone architecture for various digital interventions in the National Digital Health Mission that supports the universal health coverage.

Abstract In the present study, a sea surface temperature-based index named global-scale interdecadal variability (GIV) encompassing the combined variability of Atlantic multidecadal oscillation (AMO) and interdecadal Pacific oscillation (IPO) has been proposed. The warm phase of GIV exhibits a “cold AMO-like” pattern in the Atlantic basin and a “warm IPO-like” pattern in the Pacific basin. About 84% (R ~−0.914) of Sahelian and 42% (R ~−0.647) of Indian rainfall’s temporal variance is attributed to GIV, showing substantial improvement compared to the variance explained by AMO and IPO individually. The physical mechanism for GIV-rainfall teleconnection is related to a modification of the Walker circulation. Although there is a substantial degree of uncertainty in the current generation of state-of-the-art climate models from the Coupled Model Intercomparison Project Phase 5 (CMIP5), some still replicate the observed GIV’s spatial structure, its teleconnection, and associated physical mechanism. The results presented herein advance our knowledge about rainfall’s interdecadal variability and have imperative ramifications for developing skillful decadal predictions.

Background: Across the globe, morbidity and mortality due to non-communicable diseases (NCDs) are major public health issues. The resulting concern is not just epidemiological but also about the economic consequences at the household level. Objective: To assess the various facets of out-of-pocket spending (OOPs) incurring on NCDs, namely hypertension and diabetes on patients attending a healthcare teaching institute in Rajasthan. Methodology: This cross-sectional study involves patients older than 18 years attending either out-patient clinics or who were admitted in the wards in a healthcare teaching institute for seeking care for diabetes or hypertension. Four hundred patients were chosen purposively and a pretested questionnaire was used to elicit information on incurring OOPs for NCDs. Descriptive statistics (like percentage, mean, median, and standard deviation) were calculated. Results: The study shows a significant expenditure other than out-patient, in-patient admissions, in the form of personal expenditure and loss of employment, amounting to 31.86 and 34.07%, respectively, of the mean total expenditure. In a quarter (3 months), the mean total expenditure is ₹9014.37 ± 6452.37. On average, the OOP expenditure per visit for an out-patient visit was ₹370.54 ± 237, while for the patients admitted to the hospital, the average OOPs was ₹1564.72 ± 1310.5. Conclusions: Health expenditures can contribute toward the impoverishment of many segments of the community. Undoubtedly, numerous people may tend to neglect the needed care for NCDs due to financial hurdles. Thus, there is a need to develop NCD care management centers with health insurance packages and make them accessible for all.

Context: The Government of India under a performance-based payment scheme is providing remuneration as per the activities completed by the Accredited Social Health Activists (ASHA) health workers. Each state in India has a differentperformance-based payment system for ASHAs. The state of Rajasthan developed the ASHA-Soft system in 2014 for providing incentives to ASHAs. Aims: This study is planned to analyze the performance of ASHA-Soft considering the economic aspects as there is a paucity of studies in this context. Settings and Design: This study was conducted in Jodhpur, Rajasthan. Methods and Material: Economic analysis of ASHA-Soft program was performed based on Incentive paid to ASHA workers; Number of beneficiaries; Overall contribution of ASHAs in the health services; and unit cost per health service under ASHA-Soft program. Data of five financial years, that is, from the inception of ASHA-Soft was analyzed. Results: Consolidated incentive for maternal health, child health, and Immunization services provided to ASHAs through ASHA-Soft in the year 2015--16 was $96794.22, $35348.32, and $49016.83 which increased to $200285.00, $116320.52, and $101686.48 in the year 2019—20, respectively. Family planning and national program services were provided to 6,259 and 4,061 beneficiaries, respectively, in the year 2015--16 which markedly increased to 16,360 and 9,552 in the year 2019--20. Unit-cost of service provided by ASHAs in the National Programme ($1.13) was the lowest and for Family Planning Services ($5.71) were the highest. Conclusions: ASHA-Soft program seems a potential program to attract ASHA workers for delivering health care services. The findings of this study could assist policymakers in guiding further decision-making.

Abstract Concerted efforts are underway to establish an infrastructure for a global quantum Internet to realise a spectrum of quantum technologies. This will enable more precise sensors, secure communications, and faster data processing. Quantum communications are a front‐runner with quantum networks already implemented in several metropolitan areas. A number of recent proposals have modelled the use of space segments to overcome range limitations of purely terrestrial networks. Rapid progress in the design of quantum devices have enabled their deployment in space for in‐orbit demonstrations. We review developments in this emerging area of space‐based quantum technologies and provide a roadmap of key milestones towards a complete, global quantum networked landscape. Small satellites hold increasing promise to provide a cost effective coverage required to realise the quantum Internet. The state of art in small satellite missions is reviewed and the most current in‐field demonstrations of quantum cryptography are collated. The important challenges in space quantum technologies that must be overcome and recent efforts to mitigate their effects are summarised. A perspective on future developments that would improve the performance of space quantum communications is included. The authors conclude with a discussion on fundamental physics experiments that could take advantage of a global, space‐based quantum network.

Background: 69% of thoracic meningoceles are associated with Neurofibromatosis type 1(NF1). Pathology is debated between being a saccular protrusion of dysplastic meninges through a vertebral defect, or a forme fruste of neurofibroma, with no pathological evidence to the later. Treatment is usually conservative, however surgical intervention is sometimes indicated. Surgical options include thoracotomy, endoscopic plication of the cyst, or CSF diversion. After excision of the meningocele, watertight closure of the dura is difficult and meningoceles often reform, so dural reinforcement is indicated. Objective: A literature review on thoracic meningocele in NF1 patients: pathological hypothesis, presentation including rupture and hemothorax in large meningoceles, management strategies, and surgical challenges. The review also includes an illustrative case discussing the surgical challenges with giant meningoceles.Case presentation.A 46 year old male, NF1, with a known thoracic meningocele, presented with dyspnoea due to enlarging thoracic meningocele. The meningocele was successfully excised via thoracotomy in a challenging surgery with a novel surgical technique and unique insight on histology. Results: 10 cases of giant meningoceles in NF1 patients have been reported in literature, surgery was performed in 9 of them. 1 patient died, and there was recurrence or no change in 6 them. Histology in our illustrative case showed diffuse population of schwann cells. Also, Buttress plates achieved reinforced dural closure with no recurrence. Conclusion: Our study concluded that in in large thoracic meningocele(s) surgery via thoracotomy is advised , and dural reinforcement with buttress plates proved to be successful. Our study also concluded that meningoceles in NF1 patients is likely a forme fruste of neurofibroma, or a giant cystic neurofibroma rather than due to dysplastic dura.

A systematic compilation and evaluation of turbulent spray flame combustion data acquired from lab-scale spray-burners and CFD models validated with these are presented. Previous review studies have been limited to presentations of the progress with validation targets and modelling methods. This work expands these by presenting the details of lab-scale spray-burners and the characteristics of their datasets, the computational methodologies developed to capture the turbulent spray combustion processes acquired from the burners and their validation with the dataset. Additionally, by critically evaluating the spray and inflow boundary conditions, the reacting and non-reacting features and the main flame characteristics and data, direct correlations between the effectiveness of the dataset from the lab-scale spray burners and the outcomes from the computational methodologies using them for validation are clearly elucidated. The evaluation indicates that the turbulence modelling and validation of the swirling non-reacting flows before the application of the combustion models are important. A better representation of the multi-mode nature and the flame-turbulence-chemistry interactions in the combustion models are required. Also, a clearer experimental presentation of the flame structure and modes would help with the development of the modelling techniques. To plug these research needs, this paper reinforces the importance of developing improved numerical techniques that can be validated by selecting an appropriate lab-scale spray-burner with a robust database. Together these can be used to improve the development of and reduce emissions from combustors burning future green fuels.

Abstract Wild bees form diverse communities that pollinate plants in both native and agricultural ecosystems making them both ecologically and economically important. The growing evidence of bee declines has sparked increased interest in monitoring bee community and population dynamics using standardized methods. Here, we studied the dynamics of bee biodiversity within and across years by monitoring wild bees adjacent to four apple orchard locations in Southern Pennsylvania, USA. We collected bees using passive Blue Vane traps continuously from April to October for 6 years (2014–2019) amassing over 26,000 bees representing 144 species. We quantified total abundance, richness, diversity, composition, and phylogenetic structure. There were large seasonal changes in all measures of biodiversity with month explaining an average of 72% of the variation in our models. Changes over time were less dramatic with years explaining an average of 44% of the variation in biodiversity metrics. We found declines in all measures of biodiversity especially in the last 3 years, though additional years of sampling are needed to say if changes over time are part of a larger trend. Analyses of population dynamics over time for the 40 most abundant species indicate that about one third of species showed at least some evidence for declines in abundance. Bee family explained variation in species‐level seasonal patterns but we found no consistent family‐level patterns in declines, though bumble bees and sweat bees were groups that declined the most. Overall, our results show that season‐wide standardized sampling across multiple years can reveal nuanced patterns in bee biodiversity, phenological patterns of bees, and population trends over time of many co‐occurring species. These datasets could be used to quantify the relative effects that different aspects of environmental change have on bee communities and to help identify species of conservation concern.

Summary: Mechanisms governing regional human adipose tissue (AT) development remain undefined. Here, we show that the long non-coding RNA HOTAIR (HOX transcript antisense RNA) is exclusively expressed in gluteofemoral AT, where it is essential for adipocyte development. We find that HOTAIR interacts with polycomb repressive complex 2 (PRC2) and we identify core HOTAIR-PRC2 target genes involved in adipocyte lineage determination. Repression of target genes coincides with PRC2 promoter occupancy and H3K27 trimethylation. HOTAIR is also involved in modifying the gluteal adipocyte transcriptome through alternative splicing. Gluteal-specific expression of HOTAIR is maintained by defined regions of open chromatin across the HOTAIR promoter. HOTAIR expression levels can be modified by hormonal (estrogen, glucocorticoids) and genetic variation (rs1443512 is a HOTAIR eQTL associated with reduced gynoid fat mass). These data identify HOTAIR as a dynamic regulator of the gluteal adipocyte transcriptome and epigenome with functional importance for human regional AT development.

Obstructive sleep Apnoea (OSA) has generated lot of attention recently. The prevalence of OSA in adult Indian population is approximately 3.5% and the majority of these patients remain undiagnosed. OSA is associated with repeated episodes of hemoglobin desaturation and higher inspiratory effort, combined with repeated arousals during sleep. This causes repeated sympathetic and parasympathetic stimulation. This has been postulated to be associated with both electric and mechanical remodeling of the heart leading to cardiac arrhythmias. In this study, we try to find a correlation between OSA and cardiac arrhythmias and to find the type of arrhythmia as the secondary aim.This study was conducted over three year period at a tertiary care hospital in Mumbai. We screened 186 patients with proven OSA (Apnoea Hypopnea Index> 5). Among these, those with pre-existing structural heart disease or arrhythmia or those not consenting for the study were excluded. 121 subjects were taken up for the study. 01 was lost to follow up, leading to a final sample size of 120. These 120 patients with proven OSAand no pre- existing cardiac pathology were subjected to 24 hour ambulatory Holter monitoring to determine the prevalence of cardiac arrhythmias among this cohort. It was found that 30% of this population had some or the other arrhythmia. (n = 36). Various factors such as age, metabolic and endocrine ailments and others were also accounted for.In the follow up to this study, these 36 patients were advised to use CPAP. Following 06 months of effective CPAP use, these subjects underwent repeat 24 hour Holter examination. Unsurprisingly, significant reduction in prevalence of cardiac arrhythmia was noticed. Now, out of this cohort of 36 subjects, only 02 were found to have cardiac arrhythmia.

Diet is considered as one of the most important modifiable factors influencing human health, but efforts to identify foods or dietary patterns associated with health outcomes often suffer from biases, confounding, and reverse causation. Applying Mendelian randomization in this context may provide evidence to strengthen causality in nutrition research. To this end, we first identified 283 genetic markers associated with dietary intake in 445,779 UK Biobank participants. We then converted these associations into direct genetic effects on food exposures by adjusting them for effects mediated via other traits. The SNPs which did not show evidence of mediation were then used for MR, assessing the association between genetically predicted food choices and other risk factors, health outcomes. We show that using all associated SNPs without omitting those which show evidence of mediation, leads to biases in downstream analyses (genetic correlations, causal inference), similar to those present in observational studies. However, MR analyses using SNPs which have only a direct effect on the exposure on food exposures provided unequivocal evidence of causal associations between specific eating patterns and obesity, blood lipid status, and several other risk factors and health outcomes. Author summary Food and drink consumption is one of the most important factors influencing human health and wellbeing. The role of diet in human physiology and disease has been widely studied, but challenges in accurately assessing long term diet result in contradicting findings. Mendelian randomization is a statistical technique that uses genetic variants associated with modifiable exposures to estimate the causal effect of an exposure to a health outcome and could be extremely useful in the context of diet-health relationships. In our study, we initially identified genetic variants associated to 29 measures of food and drink consumption. We then show that genetic variants associated with food and drink consumption are subject to reverse causation and confounding. We have thus developed a statistical genetics method to identify genetic variants directly associated with food and drink consumption. By using these genetic variants (and their corresponding direct effects) in Mendelian randomization analyses we provided consistent evidence of causal associations of food and drink consumption with obesity, blood lipid status, and several other risk factors and health outcomes.

More than just a health crisis, COVID-19 pandemic has stressed across social, economical and emotional dimensions of human well being and health. The sudden enforcement although willful and honest, perpetuated a sense of insecurity and uncertainty as a result of livelihood loss, especially for the people employed in unorganized and private sectors across different urban centers of the country. The unexpected scenario not only caused widespread joblessness but also created varied conditions of psychological stresses foreseeing the less likelihood of returning of pre-COVID conditions. As an outcome of the pandemic, the involuntarily return of youth was seen as a boon for reversing the undesirable and unprecedented trends impacting the traditional rural dynamics. Present study is an attempt to highlights impacts, challenges and opportunities under and after COVID-19 on rural populace of Kailash Sacred Landscape (KSL) amid the sudden halt of remittances and jobless aspiring youth. A systematic approach was followed, where 16 villages in eight Blocks of KSL were assessed and migrants (n=815) were interviewed for compiling the information. Results revealed that COVID-19 has impacted (both positively and negatively) various sectors such as agriculture, tourism, overall family income, etc., as perceived by the respondents. Sectors like restaurants/shops, tourism, and agriculture emerged as potential cash generating avenues, with 30 %, 21 %, and 20 % respondent's perception, respectively. It is also revealed that in the absence of immediate alternatives, and policy intervention, villagers engaged themselves in the time tested traditional sectors of livelihoods or created their own niche by integrating traditional wisdom and acquired skills.

Dengue and chikungunya are two important mosquito-borne infections which are known to occur extensively in tropical and subtropical areas. Presently, there is no treatment for these viral diseases. In vitro antiviral screening of 25 extracts prepared from the plants of Vitex negundo, Plumeria alba, Ancistrocladus heyneanus, Bacopa monnieri, Anacardium occidentale, Cucurbita maxima, Simarouba glauca, and Embelia ribes using different solvents and four purified compounds (anacardic acid, chloroquinone, glaucarubinone, and methyl gallate) were carried out for their anti-dengue virus (DENV) and anti-chikungunya virus (CHIKV) activities. Maximum nontoxic concentrations of the chloroform, methanol, ethyl acetate, petroleum ether, dichloromethane, and hydroalcoholic extracts of eight plants were used. The antiviral activity was assessed by focus-forming unit assay, quantitative real-time RT-PCR, and immunofluorescence assays. Extracts from Plumeria alba, Ancistrocladus heyneanus, Bacopa monnieri, and Cucurbita maxima showed both anti-DENV and CHIKV activity while extract from Vitex negundo showed only anti-DENV activity. Among the purified compounds, anacardic acid, chloroquinone and methyl gallate showed anti-dengue activity while only methyl gallate had anti-chikungunya activity. The present study had identified the plant extracts with anti-dengue and anti-chikungunya activities, and these extracts can be further characterized for finding effective phytopharmaceutical drugs against dengue and chikungunya.

Ageing phenotypes are of great interest but are difficult to study genetically, partly due to the sample sizes required. Here, the authors present a multivariate framework to combine GWAS summary statistics and increase statistical power, identifying additional loci enriched for aging.

On the electrocardiogram, the PR interval reflects conduction from the atria to ventricles and also serves as risk indicator of cardiovascular morbidity and mortality. Here, the authors perform genome-wide meta-analyses for PR interval in multiple ancestries and identify 141 previously unreported genetic loci.

Age at voice-breaking is used to determine puberty timing in men, recall of which is considered less accurate than age at first menarche in women. Here, the authors perform multi-trait GWAS for male puberty timing by including both age at voice breaking and age of first facial hair for improved phenotype definition and power.

Abstract Background Aspergillus fumigatus, an opportunistic fungal pathogen is associated with a wide array of diseases. It produces 1, 8-dihydroxy naphthalene (DHN) melanin that imparts greenish grey color to conidia and is an important virulence factor. It masks various molecular patterns associated with A. fumigatus and protects the fungus from host immune system. Myristica fragrans, enriched with secondary metabolites has been traditionally used for the treatment of infectious and inflammatory diseases. The present study was aimed to explore the anti-melanogenic effect of M. fragrans extracts on A. fumigatus. Methods M. fragrans extracts (hexane, chloroform, methanol and ethanol) were prepared through polarity guided extraction. Phytochemical analysis was performed to detect the chemical constituents of the extracts. The minimum effective concentration (MEC) of the extracts against A. fumigatus melanin was determined by broth micro-dilution assay. Various virulence factors were assayed by spectrophotometric methods. Electron microscopic studies were performed to evaluate the effect of the hexane extract of M. fragrans on A. fumigatus cell surface morphology. The major active compounds of the extract were detected by gas chromatography-mass spectrometry (GC-MS). Docking was performed to study the interaction between the major identified compounds and the ketosynthase domain of polyketide synthase protein. Results The results indicated that the hexane extract of M. fragrans inhibited melanin production (76.09%), reduced ergosterol content (83.63%) and hydrophobicity of the cell (72.2%) at the MEC of 0.078 mg/mL. Altered conidial surface, disappearance of protrusions and absence of melanin layer on outer cell surface was observed in electron microscopy. Forty-two compounds were identified by GC-MS. The main constituents were identified as sabinene (12.2%), linoleic acid (11.7%), hexadecanoic acid (10.5%), safrole (8.1%) and elemicin (7.8%). Docking studies revealed that hexadecanoic acid, its derivative compound cis-9-hexadecenal and isoeugenol have lower binding energy forming proper hydrogen bond with ketosynthase domain of polyketide synthase protein. Conclusion The study concludes that the extract of M. fragrans has potential antifungal properties that can be explored in combination with available antifungals. This combination approach may be helpful for large number of patients suffering with A. fumigatus infections.

Background and Objectives: The procedure of removal of pathologic substances present in the plasma of the patients and its substitution with a suitable replacement fluid is known as therapeutic plasma exchange (TPE). It has been seen that this procedure has an ameliorating effect on the clinical condition of the patient of Guillain-Barré syndrome (GBS). This study aims to assess the clinical improvement in GBS patients treated with TPE at a tertiary care center. Methods: A retrospective chart review was conducted in a series of 57 GBS patients treated with TPE using an intermittent flow cell separator system. GBS disability and Medical Research Council (MRC) muscle strength scoring systems were used as final endpoints. Statistical analysis was done through the R software version 3.2.0. A value of P < 0.05 was considered to be statistically significant. Results: After 05 alternate day cycles, TPE treatment led to a significant improvement in both MRC muscle strength scores and GBS disability scores. Before TPE, the MRC muscle strength score was 1.38 ± 1.19 (range: 0–4) and this increased to 3.15 ± 1.57 (range: 0–5) after TPE, (P = 0.002). Similarly, before TPE, the mean GBS disability score was 4.00 ± 0.81 (range: 2–5) and this decreased to 2.69 ± 1.31 (range: 1–5) after TPE, (P = 0.003). Conclusion: The result of this study showed TPE to be an effective mode of treatment for GBS. The safety profile was found to be acceptable and going by the findings of this study, TPE must be considered as an early line of management.

Infectious laryngotracheitis (ILT) is a highly contagious upper respiratory tract disease of chicken caused by a Gallid herpesvirus 1 (GaHV-1) belonging to the genus Iltovirus, and subfamily Alphaherpesvirinae within Herpesviridae family. The disease is characterized by conjunctivitis, sinusitis, oculo-nasal discharge, respiratory distress, bloody mucus, swollen orbital sinuses, high morbidity, considerable mortality and decreased egg production. It is well established in highly dense poultry producing areas of the world due to characteristic latency and carrier status of the virus. Co-infections with other respiratory pathogens and environmental factors adversely affect the respiratory system and prolong the course of the disease. Latently infected chickens are the primary source of ILT virus (ILTV) outbreaks irrespective of vaccination. Apart from conventional diagnostic methods including isolation and identification of ILTV, serological detection, advanced biotechnological tools such as PCR, quantitative real-time PCR, next generation sequencing, and others are being used in accurate diagnosis and epidemiological studies of ILTV. Vaccination is followed with the use of conventional vaccines including modified live attenuated ILTV vaccines, and advanced recombinant vector vaccines expressing different ILTV glycoproteins, but still these candidates frequently fail to reduce challenge virus shedding. Some herbal components have proved to be beneficial in reducing the severity of the clinical disease. The present review discusses ILT with respect to its current status, virus characteristics, epidemiology, transmission, pathobiology, and advances in diagnosis, vaccination and control strategies to counter this important disease of poultry.

Spot blotch caused by the fungus Bipolaris sorokiniana poses a serious threat to bread wheat production in warm and humid wheat-growing regions of the world. Hence, the major objective of this study was to identify consistent genotyping-by-sequencing (GBS) markers associated with spot blotch resistance using genome-wide association mapping on a large set of 6,736 advanced bread wheat breeding lines from the International Maize and Wheat Improvement Center. These lines were phenotyped as seven panels at Agua Fria, Mexico between the 2013–2014 and 2019–2020 crop cycles. We identified 214 significant spot blotch associated GBS markers in all the panels, among which only 96 were significant in more than one panel, indicating a strong environmental effect on the trait and highlights the need for multiple phenotypic evaluations to identify lines with stable spot blotch resistance. The 96 consistent GBS markers were on chromosomes 1A, 1B, 1D, 2A, 3B, 4A, 5B, 5D, 6B, 7A, 7B, and 7D, including markers possibly linked to the Lr46, Sb1, Sb2 and Sb3 genes. We also report the association of the 2NS translocation from Aegilops ventricosa with spot blotch resistance in some environments. Moreover, the spot blotch favorable alleles at the 2NS translocation and two markers on chromosome 3BS (3B_2280114 and 3B_5601689) were associated with increased grain yield evaluated at several environments in Mexico and India, implying that selection for favorable alleles at these loci could enable simultaneous improvement for high grain yield and spot blotch resistance. Furthermore, a significant relationship between the percentage of favorable alleles in the lines and their spot blotch response was observed, which taken together with the multiple minor effect loci identified to be associated with spot blotch in this study, indicate quantitative genetic control of resistance. Overall, the results presented here have extended our knowledge on the genetic basis of spot blotch resistance in bread wheat and further efforts to improve genetic resistance to the disease are needed for reducing current and future losses under climate change.

Abstract Many countries have made ambitious pledges to increase forest areas to mitigate climate change. However, the availability of land to meet these goals is not well understood. Global studies indicate substantial potential, but do not account for local land‐use and regional variation, crucial for policy making. Using India as a case study, we use a machine learning framework to define the bioclimatic envelope of forest cover and map this against current land‐uses with varying suitability for restoration. We estimate the additional feasible area for restoration to be only 1.58 Mha, cumulatively sequestering 61.3 TgC, which is substantially less than estimates derived from global studies. However, we also find up to 14.67 Mha of opportunity for agroforestry in current agricultural land, delivering up to 98.1 TgC nationally. In the UN Decade of Restoration, we recommend developing forest restoration strategies that are compatible with existing land‐uses, such as agroforestry, especially in countries that have large smallholder agriculture holdings.

An effective and greener method for the synthesis of 2-(phenyl)-4H-chromen-4-ones has been developed by reacting 2-hydroxychalcones and tert-butyl hydroperoxide (TBHP) in the presence of a base via ultrasonic irradiation. Ultrasonic irradiation is used to replace conventional energy sources in synthesis. In terms of energy conservation and waste reduction, ultrasonic irradiations are increasingly being used as eco-friendly, green, and clean solutions in organic processes. Antifungal activity of all the synthesized compounds was evaluated against three fungi strains. Compound 4d showed the highest activity against A. fumigatus, R. stolonifer and M. mucedo with the values of 20 ​± ​0.47 ​mm, 19 ​± ​0.46 ​mm and 22 ​± ​0.49 ​mm respectively. Compound 4d also showed highest moldock score and formed H-bond with active amino acids. Compounds bearing lipophilic electron withdrawing groups showed more potent activity compared to compounds having electron donating groups.

Honey bees, Apis mellifera (Linnaeus) are crucial pollinators, however large overwinter losses of managed hives and declines in feral colonies have occurred since 2006. Increased insecticide use and the introduction of new insecticide classes have been proposed as factors contributing to these declines. Previous studies have demonstrated contact toxicity of single active ingredient insecticides to honey bees. However, the field realistic contact toxicity of formulated (pre-mix) insecticide mixtures containing multiple active ingredients (each with a different mode of action) to honey bees has not been thoroughly assessed. Here, we simulate whole body contact to spray applications made at low label recommended field rates of thiamethoxam + lambda-cyhalothrin, imidacloprid + beta-cyfluthrin, chlorantraniliprole + lambda-cyhalothrin and methoxyfenozide + spinetoram to honey bees under three realistic scenarios in field crops: (1) continuous exposure, (2) spray-only exposure, and (3) residual exposure. A customized spray tower in a laboratory setting was used to apply insecticides at the lowest recommended dose in the Environmental Protection Agency regulated label of each insecticide. Acute toxicity was determined by screening resulting mortality up to 96-hours post treatment. Mortality varied significantly between treatments at all evaluation periods for all experiments except residual exposure. For continuous and spray-only exposure, thiamethoxam + lambda-cyhalothrin and imidacloprid + beta-cyfluthrin resulted in 80–90% mortality at 48-hours, while at 96-hours we observed close to complete mortality for all treatments. This difference in mortality onset between treatments highlights the importance of extended observation beyond the 48-hours recommended by the USDA-EPA. Our finding of overall high mortality demonstrates the importance of comparing both combined and individual active ingredients in determining insecticide risk to honey bees.

Wheat blast is an emerging threat to wheat production, due to its recent migration to South Asia and Sub-Saharan Africa. Because genomic selection (GS) has emerged as a promising breeding strategy, the key objective of this study was to evaluate it for wheat blast phenotyped at precision phenotyping platforms in Quirusillas (Bolivia), Okinawa (Bolivia) and Jashore (Bangladesh) using three panels: (i) a diversity panel comprising 172 diverse spring wheat genotypes, (ii) a breeding panel comprising 248 elite breeding lines, and (iii) a full-sibs panel comprising 298 full-sibs. We evaluated two genomic prediction models (the genomic best linear unbiased prediction or GBLUP model and the Bayes B model) and compared the genomic prediction accuracies with accuracies from a fixed effects model (with selected blast-associated markers as fixed effects), a GBLUP + fixed effects model and a pedigree relationships-based model (ABLUP). On average, across all the panels and environments analyzed, the GBLUP + fixed effects model (0.63 ± 0.13) and the fixed effects model (0.62 ± 0.13) gave the highest prediction accuracies, followed by the Bayes B (0.59 ± 0.11), GBLUP (0.55 ± 0.1), and ABLUP (0.48 ± 0.06) models. The high prediction accuracies from the fixed effects model resulted from the markers tagging the 2NS translocation that had a large effect on blast in all the panels. This implies that in environments where the 2NS translocation-based blast resistance is effective, genotyping one to few markers tagging the translocation is sufficient to predict the blast response and genome-wide markers may not be needed. We also observed that marker-assisted selection (MAS) based on a few blast-associated markers outperformed GS as it selected the highest mean percentage (88.5%) of lines also selected by phenotypic selection and discarded the highest mean percentage of lines (91.8%) also discarded by phenotypic selection, across all panels. In conclusion, while this study demonstrates that MAS might be a powerful strategy to select for the 2NS translocation-based blast resistance, we emphasize that further efforts to use genomic tools to identify non-2NS translocation-based blast resistance are critical.

Wheat (Tritium aestivum L.) productivity is severely hampered by various pathogens and changing climatic conditions. Spot blotch and terminal heat stress are the major constraints of wheat production in the eastern Gangetic plains of India. To identify novel breeding sources and to understand underlying resistance mechanisms, forty-four gamma rays mutagenized wheat genotypes, derived from three different parents were screened under favourable agro-ecological conditions for spot blotch and terminal heat stress. Ten mutants showed reduced spot blotch infection calculated based on Area Under Disease Progress Curve (AUDPC), than their respective parents. The mutant TAW41 had the least infection (AUDPC: 354.32), significantly lower than its parent HD2967 (AUDPC: 675.51) and other checks. TAW41 also had a higher Normalized Difference Vegetation Index (NDVI) and chlorophyll content than the parent. Gene expression analysis of TAW41 showed differential accumulation of transcripts involved in hormonal pathways (Salicylic acid, Jasmonic acid, and ethylene) and other defense-associated genes, indicating that TAW41 might have unique resistance mechanism that facilitates this genotype to perform better against the combined stress of spot blotch and terminal heat. Hence, mutant TAW41 has been identified as a novel source of resistance that could be exploited in wheat improvement programmes to enhance tolerance to spot blotch and terminal heat stress.

Honey bees are the most important managed pollinators in commercial agriculture. Large irrigated mixed agricultural landscapes in the mid-south United States can be vital to maintaining commercial honey bee operations during times of prolonged nectar dearth, even if those crops are not dependent on bee pollinators. Severe declines in bee populations have generated concerns about the relationship of pesticide-treated seeds and crops and the health of honey bees. To investigate the role of pollen from seed-treated crops as a component of honey bee diet and pesticide contamination in pollen diet of honey bees from agricultural and urban landscapes, we monitored honey bee colonies in both agricultural and urban areas. Pollen collection began in mid-March, before seed-treated crops were planted, and continued through the end of August, after crops had ceased blooming. Pollen samples from returning bees were identified to determine the botanical origin of the bees’ pollen diet, and were also analyzed for pesticide contamination. Honey bees in the agricultural landscape visited crop sources only during the seasonal period of natural nectar dearth, when other wildflower sources were limited, and they encountered acutely toxic pesticide residues (above LD50) during this period. These bees also encountered toxic levels of herbicide on multiple occasions throughout the season, in pollen from non-crop plants. Bees in the urban area were also exposed to toxic levels of insecticides on several occasions. Urban bees were exposed to herbicides throughout the season, but not at concentrations approaching acute toxicity. Simultaneous exposure to multiple pesticides occurred at both sites on multiple occasions. The results underscore the need to conserve areas of habitat and forage for both wild and managed pollinators within both agricultural and urban landscapes.

The growth in the global energy consumption demands an increasing use of the renewable energy sources. Nevertheless, fossil fuels are projected to remain as the dominating backbone of petrochemical and consumer industry in the coming decades. As a bridge from the fossil fuel-based to renewable fuel-based economy, chemical looping technology has been developed to achieve sustainable applications by generating highly efficient energy and products through economically viable processes. This article discusses the advances in chemical looping with a focus on the fundamentals of formulation concepts on chemical looping carriers, rationale for reactor configurations, and design consideration from the process analysis. Particularly, the carriers utilized for different chemical looping applications ranging from syngas generation to ammonia synthesis are elaborated. The reactor configurations applied for different chemical looping systems are discussed along with the essentials of the process simulation approach to system optimization. The article also describes the prospects of the future development in the chemical looping technology, and its viability and readiness for industrial deployment.

Inbreeding depression has been observed in many different species, but in humans a systematic analysis has been difficult so far. Here, analysing more than 1.3 million individuals, the authors show that a genomic inbreeding coefficient (FROH) is associated with disadvantageous outcomes in 32 out of 100 traits tested.

Spot blotch caused by Bipolaris sorokiniana has spread to more than 9 million ha of wheat in the warm, humid areas of the Eastern Gangetic Plains (EGP) of South Asia and is a disease of major concern in other similar wheat growing regions worldwide. Differential lignin content in resistant and susceptible genotypes and its association with free radicals such as hydrogen peroxide (H2O2), superoxide (O2−) and hydroxyl radical (OH−) were studied after inoculation under field conditions for two consecutive years. H2O2 significantly influenced lignin content in flag leaves, whereas there was a negative correlation among lignin and H2O2 to the Area Under Disease Progress Curve (AUDPC). The production of H2O2 was higher in the resistant genotypes than susceptible ones. The O2− and OH− positively correlated with AUDPC but negatively with lignin content. This study illustrates that H2O2 has a vital role in prompting lignification and thereby resistance to spot blotch in wheat. We used cluster analysis to separate the resistant and susceptible genotypes by phenotypic and biochemical traits. H2O2 associated lignin production significantly reduced the number of appressoria and penetration pegs. We visualized the effect of lignin in disease resistance using differential histochemical staining of tissue from resistant and susceptible genotypes, which shows the variable accumulation of hydrogen peroxide and lignin around penetration sites.

Crop yield estimation before harvest is required for marketing, pricing, storage, import, export etc. Productivity of cropping systems under various weather, management and policy scenarios can be predicted successfully by simulation models. Due to increase in input cost of agricultural operation, agriculture produces become costly. Therefore, crop yield estimation in the agriculture becomes essential. Weather variability causes the losses in the yield. Therefore, model based on weather parameters, soil parameter and crop parameters can provide reliable crop yield estimation in advance. For estimating the multi stage wheat crop yield, experiments were conducted at research farm of IARI, New Delhi during Rabi 2016–17 and Rabi 2017–18. Crop yield were estimated by weather based and crop simulation model. Percentage deviation of estimated yield by observed yield at flowering and grain filling stage was −5.1 and 2.0 by weather based model, 4.3 and 2.1 by InfoCrop model, 10.2 and 9.0 by DSSAT model during Rabi 2016–17 and 5.3 and 5.9 by weather based model, 2.3 and 2.2 by InfoCrop model, −10.8 and −9.6 by DSSAT model during Rabi 2017–18 respectively. Among the three models opted for estimating the yield at flowering and grain filling stage, InfoCrop model gave better results followed by weather based and DSSAT model. Therefore, this model can be used for multi stage wheat crop yield estimation at district as well as regional level.

Christina M. Lill, who contributed to analysis of data, was inadvertently omitted from the author list in the originally published version of this article. This has now been corrected in both the PDF and HTML versions of the article.

Interventions early in life are the need of the hour when it comes to controlling the rising incidence of communicable and non-communicable diseases (NCDs) globally. WHO has issued guidelines towards health promotional initiatives at schools as a part of Global School Health Initiative, and the Government of India has directed many policies and programs to integrate health deep within the school activities. School Health Promotion is an international need with programmes implementing across continents due to numerous documented benefits, to not just the individuals but to the community and country as a whole. Simple teachings like hand hygiene have shown to reduce the incidence diarrhea by more than 50% amongst children (a major cause of mortality in India), thus raising an urgent need of developing a model for health promotion at schools that is replicable, sustainable, and can be modified to the local needs as well. Though the existent programmes have a few documented challenges, a multisectorial involvement of government agencies, educational boards, and health sector along with the school is the way forward to address those challenges and covert the theory of health promoting schools (HPS) into a well-established fact. It presents a scope for the various established and newly emerging Schools of Public Health in the country to come forward and collaborate with these multiple sectors. These collaborations can be the only way to ensure sustainability and incorporation of health promotion into the core academic structure of schools in a diverse and highly populous country like India.

Wheat blast (WB) is a destructive disease in South America and its first outbreak in Bangladesh in 2016 posed a great risk to food security of South Asian countries. A genome wide association study (GWAS) was conducted on a diverse panel of 184 wheat genotypes from South Asia and CIMMYT. Phenotyping was conducted in eight field experiments in Bolivia and Bangladesh and a greenhouse experiment in the United States. Genotypic data included 11,401 SNP markers of the Illumina Infinium 15K BeadChip and four additional STS markers on the 2NS/2AS translocation region. Accessions with stable WB resistance across experiments were identified, which were all 2NS carriers. Nevertheless, a dozen moderately resistant 2AS lines were identified, exhibiting big variation among experiments. Significant marker-trait associations (MTA) were detected on chromosomes 1BS, 2AS, 6BS, and 7BL; but only MTAs on 2AS at the 2NS/2AS translocation region were consistently significant across experiments. The resistant accessions identified in this study could be used in production in South Asian countries as a preemptive strategy to prevent WB outbreak.

Background: Lung function is highly heritable and differs between the sexes throughout life. However, little is known about sex-differential genetic effects on lung function. We aimed to conduct the first genome-wide genotype-by-sex interaction study on lung function to identify genetic effects that differ between males and females. Methods: We tested for interactions between 7,745,864 variants and sex on spirometry-based measures of lung function in UK Biobank (N=303,612), and sought replication in 75,696 independent individuals from the SpiroMeta consortium. Results: Five independent single-nucleotide polymorphisms (SNPs) showed genome-wide significant (P

Spot blotch (SB) disease causes significant yield loss in wheat production in the warm and humid regions of the eastern Gangetic plains (EGP) of South Asia (SA). Most of the cultivated varieties in the eastern part of SA are affected by SB under favorable climatic conditions. To understand the nature of SB resistance and map the underlying resistant loci effective in SA, two bi-parental mapping populations were evaluated for 3 years, i.e., 2013–2015 for the BARTAI × CIANO T79 population (denoted as BC) and 2014–2016 for the CASCABEL × CIANO T79 population (CC), at Varanasi, Uttar Pradesh, India. DArTSeq genotyping-by-sequencing (GBS) platform was used for genotyping of the populations. Distribution of disease reaction of genotypes in both populations was continuous, revealing the quantitative nature of resistance. Significant “genotype,” “year,” and “genotype × year” interactions for SB were observed. Linkage map with the genome coverage of 8,598.3 and 9,024.7 cM in the BC and CC population, respectively, was observed. Two quantitative trait loci (QTLs) were detected on chromosomes 1A and 4D in the BC population with an average contribution of 4.01 and 12.23% of the total phenotypic variation (PV), respectively. Seven stable QTLs were detected on chromosomes 1B, 5A, 5B, 6A, 7A, and 7B in the CC population explaining 2.89–10.32% of PV and collectively 39.91% of the total PV. The QTL detected at the distal end of 5A chromosome contributed 10.32% of the total PV. The QTLs on 6A and 7B in CC could be new, and the one on 5B may represent the Sb2 gene. These QTLs could be used in SB resistance cultivar development for SA.

Colors are added to food items to make them more attractive and appealing. Food colorants therefore, have an impressive market due to the requirements of food industries. A variety of synthetic coloring agents approved as food additives are available and being used in different types of food prepared or manufactured worldwide. However, there is a growing concern that the use of synthetic colors may exert a negative impact on human health and environment in the long run. The natural pigments obtained from animals, plants, and microorganisms are a promising alternative to synthetic food colorants. Compared to animal and plant sources, microorganisms offer many advantages such as no seasonal impact on the quality and quantity of the pigment, ease of handling and genetic manipulation, amenability to large scale production with little or no impact on biodiversity etc. Among the microorganisms algae, fungi and bacteria are being used to produce pigments as food colorants. This review describes the types of microbial food pigments in use, their benefits, production strategies, and associated challenges.

The ever-growing genome-wide association studies (GWAS) have revealed widespread pleiotropy. To exploit this, various methods that jointly consider associations of a genetic variant with multiple traits have been developed. Most efforts have been made concerning improving GWAS discovery power. However, how to replicate these discovered pleiotropic loci has yet to be discussed thoroughly. Unlike a single-trait scenario, multi-trait replication is not trivial considering the underlying genotype-multi-phenotype map of the associations. Here, we evaluate four methods for replicating multi-trait associations, corresponding to four levels of replication strength. Weak replication cannot justify pleiotropic genetic effects, whereas strong replication using our developed correlation methods can inform consistent pleiotropic genetic effects across the discovery and replication samples. We provide a protocol for replicating multi-trait genetic associations in practice. The described methods are implemented in the free and open-source R package MultiABEL.