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33 results on '"Pérez-Cabezas, Begoña"'

1. MAEBL Contributes to Plasmodium Sporozoite Adhesiveness

Author

Sá, Mónica, primary, Costa, David Mendes, additional, Teixeira, Ana Rafaela, additional, Pérez-Cabezas, Begoña, additional, Formaglio, Pauline, additional, Golba, Sylvain, additional, Sefiane-Djemaoune, Hélèna, additional, Amino, Rogerio, additional, and Tavares, Joana, additional

Published
2022
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2. Immunization with CSP and a RIG-I Agonist is Effective in Inducing a Functional and Protective Humoral Response Against Plasmodium

Author

Teixeira, Ana Rafaela, primary, Pérez-Cabezas, Begoña, additional, Costa, David M., additional, Sá, Mónica, additional, Golba, Sylvain, additional, Sefiane-Djemaoune, Hélèna, additional, Ribeiro, Joana, additional, Kaneko, Izumi, additional, Iwanaga, Shiroh, additional, Yuda, Masao, additional, Tsuji, Moriya, additional, Boscardin, Silvia Beatriz, additional, Amino, Rogerio, additional, Cordeiro-da-Silva, Anabela, additional, and Tavares, Joana, additional

Published
2022
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3. Downregulation of the secreted protein with an altered thrombospondin repeat (SPATR) impacts the infectivity of malaria sporozoites

Author

Costa, David Mendes, primary, Sá, Mónica, additional, Teixeira, Ana Rafaela, additional, Pérez-Cabezas, Begoña, additional, Golba, Sylvain, additional, Sefiane-Djemaoune, Hélèna, additional, Formaglio, Pauline, additional, Franke-Fayard, Blandine, additional, Janse, Chris J., additional, Amino, Rogerio, additional, and Tavares, Joana, additional

Published
2022
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5. Protective Efficacy in a Hamster Model of a Multivalent Vaccine for Human Visceral Leishmaniasis (MuLeVaClin) Consisting of the KMP11, LEISH-F3+, and LJL143 Antigens in Virosomes, Plus GLA-SE Adjuvant

Author

Fernández, Laura, primary, Solana, Jose Carlos, additional, Sánchez, Carmen, additional, Jiménez, Mª Ángeles, additional, Requena, Jose M., additional, Coler, Rhea, additional, Reed, Steven G., additional, Valenzuela, Jesus G., additional, Kamhawi, Shaden, additional, Oliveira, Fabiano, additional, Fichera, Epifanio, additional, Glueck, Reinhard, additional, Bottazzi, Maria Elena, additional, Gupta, Gaurav, additional, Cecilio, Pedro, additional, Pérez-Cabezas, Begoña, additional, Cordeiro-da-Silva, Anabela, additional, Gradoni, Luigi, additional, Carrillo, Eugenia, additional, and Moreno, Javier, additional

Published
2021
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6. Protective efficacy in a hamster model of a multivalent vaccine for human visceral leishmaniasis (Mulevaclin) consisting of the kmp11, leish-f3+, and ljl143 antigens in virosomes, plus gla-se adjuvant

Author

European Commission, Red de Investigación Cooperativa en Enfermedades Tropicales (España), Fernández, Laura, Solana, José Carlos, Sánchez, Carmen, Jiménez, M. Angeles, Requena, José María, Coler, Rhea, Reed, Steven G., Valenzuela, Jesus G., Kamhawi, Shaden, Oliveira, Fabiano, Fichera, Epifanio, Glueck, Reinhard, Bottazzi, María Elena, Gupta, Gaurav, Cecilio, Pedro, Pérez-Cabezas, Begoña, Cordeiro-da-Silva, Anabela, Gradoni, Luigi, Carrillo, Eugenia, Moreno, Javier, European Commission, Red de Investigación Cooperativa en Enfermedades Tropicales (España), Fernández, Laura, Solana, José Carlos, Sánchez, Carmen, Jiménez, M. Angeles, Requena, José María, Coler, Rhea, Reed, Steven G., Valenzuela, Jesus G., Kamhawi, Shaden, Oliveira, Fabiano, Fichera, Epifanio, Glueck, Reinhard, Bottazzi, María Elena, Gupta, Gaurav, Cecilio, Pedro, Pérez-Cabezas, Begoña, Cordeiro-da-Silva, Anabela, Gradoni, Luigi, Carrillo, Eugenia, and Moreno, Javier

Abstract

Visceral leishmaniasis (VL) is the most severe clinical form of leishmaniasis, fatal if untreated. Vaccination is the most cost-effective approach to disease control; however, to date, no vaccines against human VL have been made available. This work examines the efficacy of a novel vaccine consisting of the Leishmania membrane protein KMP11, LEISH-F3+ (a recombinant fusion protein, composed of epitopes of the parasite proteins nucleoside hydrolase, sterol-24-c-methyltransferase, and cysteine protease B), and the sand fly salivary protein LJL143, in two dose ratios. The inclusion of the TLR4 agonist GLA-SE as an adjuvant, and the use of virosomes (VS) as a delivery system, are also examined. In a hamster model of VL, the vaccine elicited antigen-specific immune responses prior to infection with Leishmania infantum. Of note, the responses were greater when higher doses of KMP11 and LEISH-F3+ proteins were administered along with the GLA-SE adjuvant and/or when delivered within VS. Remarkably, hamsters immunized with the complete combination (i.e., all antigens in VS + GLA-SE) showed significantly lower parasite burdens in the spleen compared to those in control animals. This protection was underpinned by a more intense, specific humoral response against the KMP11, LEISH-F3+, and LJL143 antigens in vaccinated animals, but a significantly less intense antibody response to the pool of soluble Leishmania antigens (SLA). Overall, these results indicate that this innovative vaccine formulation confers protection against L. infantum infection, supporting the advancement of the vaccine formulation into process development and manufacturing and the conduction of toxicity studies towards future phase I human clinical trials

Published
2021

9. IL-2 and IFN-γ are biomarkers of SARS-CoV-2 specific cellular response in whole blood stimulation assays

Author

Pérez-Cabezas, Begoña, primary, Ribeiro, Ricardo, additional, Costa, Inês, additional, Esteves, Sofia, additional, Teixeira, Ana Rafaela, additional, Reis, Teresa, additional, Monteiro, Ricardo, additional, Afonso, Alexandre, additional, Pinheiro, Vitor, additional, Antunes, Maria Isabel, additional, Lucília Araújo, Maria, additional, Ribeiro, João Niza, additional, Cordeiro-da-Silva, Anabela, additional, Santarém, Nuno, additional, and Tavares, Joana, additional

Published
2021
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11. Lipid Antigen Presentation by CD1b and CD1d in Lysosomal Storage Disease Patients

Author

Pereira, Catia S., primary, Pérez-Cabezas, Begoña, additional, Ribeiro, Helena, additional, Maia, M. Luz, additional, Cardoso, M. Teresa, additional, Dias, Ana F., additional, Azevedo, Olga, additional, Ferreira, M. Fatima, additional, Garcia, Paula, additional, Rodrigues, Esmeralda, additional, Castro-Chaves, Paulo, additional, Martins, Esmeralda, additional, Aguiar, Patricio, additional, Pineda, Mercè, additional, Amraoui, Yasmina, additional, Fecarotta, Simona, additional, Leão-Teles, Elisa, additional, Deng, Shenglou, additional, Savage, Paul B., additional, and Macedo, M. Fatima, additional

Published
2019
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15. Pre-clinical antigenicity studies of an innovative multivalent vaccine for human visceral leishmaniasis

Author

Cecílio, Pedro, primary, Pérez-Cabezas, Begoña, additional, Fernández, Laura, additional, Moreno, Javier, additional, Carrillo, Eugenia, additional, Requena, José M., additional, Fichera, Epifanio, additional, Reed, Steven G., additional, Coler, Rhea N., additional, Kamhawi, Shaden, additional, Oliveira, Fabiano, additional, Valenzuela, Jesus G., additional, Gradoni, Luigi, additional, Glueck, Reinhard, additional, Gupta, Gaurav, additional, and Cordeiro-da-Silva, Anabela, additional

Published
2017
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17. Poly-N-acetyl glucosamine expression in Staphylococcus epidermidis biofilm cells affects the immune response and promotes tissue pathology in infected hosts

Author

Ferreirinha, Pedro, Pérez-Cabezas, Begoña, Correia, Alexandra, Miyazawa, Bruna, Carvalhais, Virgínia Maria Dinis, França, Ângela Maria Oliveira Sousa, Faustinho, Augusto, Cordeiro-da-Silva, Anabela, Teixeira, Luzia, Pier, Geral, Cerca, Nuno, Vilanova, Manuel, and Universidade do Minho

Subjects
Ciências Médicas::Biotecnologia Médica
Abstract

Staphylococcus epidermidis is a biofilm - forming bacterium and a leading etiological agent of nosocomial infections. The ability to establish biofilms on indwelling medical devices is a key virulence factor for this bacterium. Still, the influence of poly - N - acetyl glucosamine (PNAG), the major component of the extracellular biofilm matrix, in the host immune response has been scarcely studied. Here, t h is influence was assessed in mice challenged i.p. with PNAG - p roducing (WT) and isogenic - mutant lacking PNAG (M10) bacteria grown in biofilm - inducing conditions. Faster bacterial clearance was observed in the mice infected with WT bacteria than in M10 - infected counterparts , which w as accompanied by earlier neutrophil recruitment and higher IL - 6 production. Interestingly, in the WT - infected mice, but not in those infected with M10 , elevated serum IL - 10 was detected . To further study the effe ct of PNAG in the immune response, mice were primed with WT or M10 biofilm bacteria and subsequently infected with WT biofilm - released cells. WT - primed mice presented a higher frequency of splenic IFN - γ + and IL - 17 + CD4 + T cells, and more severe liver patho logy than M10 - primed counterparts. Nevertheless, T reg cells obtained from the WT - primed mice presented a higher suppressive function than those obtained from M10 - primed mice. This effect was abrogated when IL - 10 - deficient mice were similarly primed and infected indicating that PNAG promotes the differentiati on of highly suppressive T reg cells by a mechanism dependent on IL - 10. Altogether, these results provide evidence help ing explain ing the coexistence of inflammation and bacterial persistence often observed in biofilm - originated S. epidermidis infections, FCT and COMPETE grants PTDC/BIA-MIC/113450/2009 and FCOMP-01-0124-FEDER-14309. PF, AC, LT and VC were supported by FCT grants SFRH/BD/76900/2011, SFRH/BPD/91623/2012, IF/01241/2014, SFRH/BD/78235/2011

Published
2015

19. Poly- N -Acetylglucosamine Production by Staphylococcus epidermidis Cells Increases Their In Vivo Proinflammatory Effect

Author

Ferreirinha, Pedro, primary, Pérez-Cabezas, Begoña, additional, Correia, Alexandra, additional, Miyazawa, Bruna, additional, França, Angela, additional, Carvalhais, Virgínia, additional, Faustino, Augusto, additional, Cordeiro-da-Silva, Anabela, additional, Teixeira, Luzia, additional, Pier, Gerald B., additional, Cerca, Nuno, additional, and Vilanova, Manuel, additional

Published
2016
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22. Human scavenger protein AIM increases foam cell formation and CD36-mediated oxLDL uptake

Author

Amézaga, Núria, primary, Sanjurjo, Lucía, additional, Julve, Josep, additional, Aran, Gemma, additional, Pérez-Cabezas, Begoña, additional, Bastos-Amador, Patricia, additional, Armengol, Carolina, additional, Vilella, Ramon, additional, Escolà-Gil, Joan Carles, additional, Blanco-Vaca, Francisco, additional, Borràs, Francesc E, additional, Valledor, Annabel F, additional, and Sarrias, Maria-Rosa, additional

Published
2013
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25. DC2010: Forum on Vaccine Science

Author

Bastos, Patricia, primary, Benitez-Ribas, Daniel, additional, Crisci, Elisa, additional, Naranjo, Mar, additional, Pérez-Cabezas, Begoña, additional, and Montoya, Maria, additional

Published
2011
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29. Poly-N-Acetylglucosamine Production by Staphylococcus epidermidisCells Increases Their In VivoProinflammatory Effect

Author

Ferreirinha, Pedro, Pérez-Cabezas, Begoña, Correia, Alexandra, Miyazawa, Bruna, França, Angela, Carvalhais, Virgínia, Faustino, Augusto, Cordeiro-da-Silva, Anabela, Teixeira, Luzia, Pier, Gerald B., Cerca, Nuno, and Vilanova, Manuel

Abstract

ABSTRACTPoly-N-acetylglucosamine (PNAG) is a major component of the Staphylococcus epidermidisbiofilm extracellular matrix. However, it is not yet clear how this polysaccharide impacts the host immune response and infection-associated pathology. Faster neutrophil recruitment and bacterial clearance were observed in mice challenged intraperitoneally with S. epidermidisbiofilm cells of the PNAG-producing 9142 strain than in mice similarly challenged with the isogenic PNAG-defective M10 mutant. Moreover, intraperitoneal priming with 9142 cells exacerbated liver inflammatory pathology induced by a subsequent intravenous S. epidermidischallenge, compared to priming with M10 cells. The 9142-primed mice had elevated splenic CD4+T cells producing gamma interferon and interleukin-17A, indicating that PNAG promoted cell-mediated immunity. Curiously, despite having more marked liver tissue pathology, 9142-primed mice also had splenic T regulatory cells with greater suppressive activity than those of their M10-primed counterparts. By showing that PNAG production by S. epidermidisbiofilm cells exacerbates host inflammatory pathology, these results together suggest that this polysaccharide contributes to the clinical features associated with biofilm-derived infections.

Published
2016
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30. Deception and manipulation: the arms of Leishmania, a successful parasite.

Author

Cecílio, Pedro, Pérez-Cabezas, Begoña, Santarém, Nuno, Maciel, Joana, Rodrigues, Vasco, and da Silva, Anabela Cordeiro

Subjects
LEISHMANIA, PARASITES, IMMUNOREGULATION, NATURAL immunity, DISEASE progression
Abstract

Leishmania spp. are intracellular parasitic protozoa responsible for a group of neglected tropical diseases, endemic in 98 countries around the world, called leishmaniasis. These parasites have a complex digenetic life cycle requiring a susceptible vertebrate host and a permissive insect vector, which allow their transmission. The clinical manifestations associated with leishmaniasis depend on complex interactions between the parasite and the host immune system. Consequently, leishmaniasis can be manifested as a self-healing cutaneous affliction or a visceral pathology, being the last one fatal in 85-90% of untreated cases. As a result of a long host-parasite co-evolutionary process, Leishmania spp. developed different immunomodulatory strategies that are essential for the establishment of infection. Only through deception and manipulation of the immune system, Leishmania spp. can complete its life cycle and survive. The understanding of the mechanisms associated with immune evasion and disease progression is essential for the development of novel therapies and vaccine approaches. Here, we revise how the parasite manipulates cell death and immune responses to survive and thrive in the shadow of the immune system. [ABSTRACT FROM AUTHOR]

Published
2014
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31. Human scavenger protein AIM increases foam cell formation and CD36‐mediated oxLDL uptake

Author

Amézaga, Núria, Sanjurjo, Lucía, Julve, Josep, Aran, Gemma, Pérez‐Cabezas, Begoña, Bastos‐Amador, Patricia, Armengol, Carolina, Vilella, Ramon, Escolà‐Gil, Joan Carles, Blanco‐Vaca, Francisco, Borràs, Francesc E., Valledor, Annabel F., and Sarrias, Maria‐Rosa

Abstract

Human scavenger protein AIM is involved in macrophage survival, adhesion, and foam cell formation, contributing to atherosclerosis‐related mechanisms in the macrophage. AIM is expressed by macrophages in response to agonists of the nuclear receptors LXR/RXR. In mice, it acts as an atherogenic factor by protecting macrophages from the apoptotic effects of oxidized lipids. In humans, it is detected in atherosclerotic lesions, but no role related to atherosclerosis has been reported. This study aimed to investigate whether the role of hAIM extends beyond inhibiting oxidized lipid‐induced apoptosis. To accomplish this goal, functional analysis with human monocytic THP1 cells and macrophages differentiated from peripheral blood monocytes were performed. It was found that hAIM reduced oxLDL‐induced macrophage apoptosis and increased macrophage adhesion to endothelial ICAM‐1 by enhancing LFA‐1 expression. Furthermore, hAIM increased foam cell formation, as shown by Oil Red O and Nile Red staining, as well as quantification of cholesterol content. This was not a result of decreased reverse cholesterol transport, as hAIM did not affect the efflux significantly from [3H] Cholesterol‐laden macrophages driven by plasma, apoA‐I, or HDL2 acceptors. Rather, flow cytometry studies indicated that hAIM increased macrophage endocytosis of fluorescent oxLDL, which correlated with an increase in the expression of the oxLDLR CD36. Moreover, hAIM bound to oxLDL in ELISA and enhanced the capacity of HEK‐293 cells expressing CD36 to endocytose oxLDL, as studied using immunofluorescence microscopy, suggesting that hAIM serves to facilitate CD36‐mediated uptake of oxLDL. Our data represent the first evidence that hAIM is involved in macrophage survival, adhesion, and foam cell formation and suggest a significant contribution to atherosclerosis‐related mechanisms in the macrophage.

Published
2014
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32. Estudo da expressão CDC5 e CDC6 en células iNKT humanas

Author

Cunha, Ana Margarida Delindro Ferreira da, Macedo, Maria de Fátima Matos Almeida Henriques, and Pérez-Cabezas , Begoña

Subjects
Antigen, Antigen presenting cells, T cells, CD6, TCR, Invariant Natural Killer T cells, CD5
Abstract

Submitted by Cristina Santos (cmaria@ua.pt) on 2019-01-24T11:53:22Z No. of bitstreams: 1 Documento.pdf: 2845453 bytes, checksum: aecc9acd3f838c221f2d515e5e4644cb (MD5) Made available in DSpace on 2019-01-24T11:53:22Z (GMT). No. of bitstreams: 1 Documento.pdf: 2845453 bytes, checksum: aecc9acd3f838c221f2d515e5e4644cb (MD5) Previous issue date: 2018-12-21 Mestrado em Biomedicina Molecular

Published
2018

33. Study of CDC5 and CDC6 expression on human iNKT cells

Author

Cunha, Ana Margarida Delindro Ferreira da, Macedo, Maria de Fátima Matos Almeida Henriques, and Pérez-Cabezas , Begoña

Subjects
Antigen, Antigen presenting cells, T cells, CD6, TCR, Invariant Natural Killer T cells, CD5
Abstract

Invariant Natural Killer (iNKT) cells are T lymphocytes that recognize lipid antigens presented by CD1d molecules and have a semi-invariant T cell receptor. iNKT cells produce high quantities of cytokines after antigen recognition or activation. The regulation of iNKT cell activation is crucial for its role in infection and tumor control. CD5 and CD6 are transmembrane surface glycoproteins expressed by conventional T cells that regulate the activation of these cells, but their expression on human iNKTs has not been investigated yet. Here, we studied the basal expression of both CD5 and CD6 on human iNKTs and also their modulation after activation. The involvement of CD6 in antigen induced iNKT cell activation was also addressed. Our results show that human peripheral blood iNKT cells express both CD5 and CD6, at similar and higher levels, respectively, than conventional T cells. Activation of a human iNKT cell line with the non-specific stimulus Phytohaemagglutinin (PHA) down-regulated the expression of both CD5 and CD6, whereas when the prototypic antigen α-Galactosylceramide (α-GalCer) was used only a decrease in CD6 expression was observed. The use of Raji B cells, expressing or not CD166, a ligand for CD6, as antigen presenting cells did not disclose a major role for CD6 in α-GalCer induced iNKT cell activation As células Invariant Natural killer T (iNKT) são um subtipo de linfócitos T que reconhecem antigénios lipídicos através da molecula de CD1d e têm um recetor de células T semi-invariante. Estas células produzem grandes quantidades de citocinas após serem ativadas. A regulação das iNKTs é crucial para controlar o seu efeito na infeção e no cancro. CD5 e CD6 são glicoproteínas transmembranares expressas na superfície das células T e que regulam a sua activação, sendo que a sua expressão em células iNKTs humanas ainda não tinha sido estudada. Neste trabalho estudou-se a expressão basal destas moléculas em iNKTs humanas e também a sua modelação após activação. O envolvimento do CD6 na indução de ativação das iNKTs pelo antigénio α-Galactosylceramide (α-GalCer) também foi estudada. Os resultados mostraram que as iNKTs presentes no sangue humano periférico expressam CD5 e CD6, sendo que em comparação com as células T convencionais, o CD5 é expresso a níveis semelhantes e o CD6 é expresso em níveis superiores. A activação de iNKTs humanas pela Phytohaemagglutinin (PHA) (estímulo não específico) diminuiu a expressão das moléculas CD5 e CD6 enquanto que com o antigénio prototípico α-GalCer só se verificou uma descida na expressão de CD6. O uso de células Raji expressando ou não o CD166, um ligando do CD6, como células apresentadoras de antigénio, não revelou ter um papel importante na indução de activação da iNKTs pelo antigénio α-GalCer Mestrado em Biomedicina Molecular

Published
2018

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