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Axonotmesis causes sensorimotor and neurofunctional deficits, and its regeneration can occur slowly or not occur if not treated appropriately. Low-level laser therapy (LLLT) promotes nerve regeneration with the proliferation of myelinating Schwann cells to recover the myelin sheath and the production of glycoproteins for endoneurium reconstruction. This study aimed to evaluate the effects of LLLT on sciatic nerve regeneration after compression injury by means of the sciatic functional index (SFI) and Raman spectroscopy (RS). For this, 64 Wistar rats were divided into two groups according to the length of treatment: 14 days (n = 32) and 21 days (n = 32). These two groups were subdivided into four sub-groups of eight animals each (control 1; control 2; laser 660 nm; laser 808 nm). All animals had surgical exposure to the sciatic nerve, and only control 1 did not suffer nerve damage. To cause the lesion in the sciatic nerve, compression was applied with a Kelly clamp for 6 s. The evaluation of sensory deficit was performed by the painful exteroceptive sensitivity (PES) and neuromotor tests by the SFI. Laser 660 nm and laser 808 nm sub-groups were irradiated daily (100 mW, 40 s, energy density of 133 J/cm

Introduction: The cadaver is an irreplaceable piece in the learning of human anatomy, allowing a three-dimensional view of anatomical structures. However, there is a scenario of scarcity and increased demand for cadaveric parts in Brazilian universities. Therefore, several institutions, supported by article 14 of Law 10.406/2002 of the Brazilian Civil Code, implemented body donation programs aimed at bringing significant improvements in the teaching quality of the anatomy discipline. Objective: To analyze the level of knowledge and perspectives of a university community regarding the voluntary donation of cadaveric bodies for educational and research purposes. Method: This is an observational, cross-sectional and prospective study with a quantitative approach and data collection through an individual questionnaire. The subjects included in the study were teachers and students from the Federal University of Maranhão, Campus Dom Delgado - UFMA. The questionnaires were related to obtaining information about knowledge and opinions on the topic of body donation. Result: A total of 264 answered questionnaires were obtained, which showed that 85.65% of students and 87.1% of teachers were aware of the possibility of donating their own bodies. However, it was observed that 94% of the interviewees did not know what procedures were necessary for the donation, and 86% did not have knowledge about the legislation that allowed this practice. Furthermore, 27.65% of the participants were able to donate their own body, 33.7% indicated that they would not donate and 38.9% reported that they had no formed opinion. The main reason given for donating was: “to contribute to the advancement of education in the health area”, and for not donating: “I want to be buried or cremated”. Conclusion: There is a high percentage of individuals who do not know how to carry out the donation of their own bodies, being evidenced that the participants have low aptitude for donation. However, there is a significant percentage of individuals who do not have a formed opinion on the subject, demonstrating that the dissemination of this topic is a means to increase adherence to this practice. It is important to carry out the development of body donation programs to solve the problem.

The aim of this study was to identify biochemical changes in sciatic nerve (SN) after crush injury and low-level laser therapy (LLLT) with 660 nm and 808 nm by Raman spectroscopy (RS) analysis. A number of 32 Wistar rats were used, divided into four groups (control 1, control 2, LASER 660 nm, and LASER 808 nm). All animals underwent surgical procedure of the SN and groups control 2, LASER 660 nm, and LASER 808 nm were submitted to SN crush damage (axonotmesis). The LLLT in the groups LASER 660 nm and LASER 808 nm was applied daily for 21 consecutive days (100 mW, 30 s, 133 J/cm2 fluence). The hind paw was removed and the SN was dissected and positioned on an aluminum support to collect dispersive Raman spectra (830 nm excitation, 30 s accumulation). To estimate the biochemical changes in the SN associated with LLLT, the principal component analysis (PCA) was applied. The Raman spectra of the sciatic nerve fragments showed peaks of the major biochemical components of the nerve, especially sphingolipids, phospholipids, glycoproteins, and collagen. The spectral features identified in some of the principal component loading vectors are referred to the biochemical elements present on the SN and were increased in the groups treated with LLLT, mainly lipids (sphingo and phospholipids) and proteins (collagen)—constituents of the myelin sheath. The RS was effective in identifying the biochemical differences in the SN after the crush injury, and LASER 660 nm was more efficient than the LASER 808 nm in cell proliferation and repair of the injured SN.

Background: Extended vertical gastrectomy is a variation of the vertical gastrectomy technique requiring studies to elucidate safety in relation to gastroesophageal reflux. Aim: To analyze comparatively vertical gastrectomy (VG) and extended vertical gastrectomy (EVG) in rats with obesity induced by cafeteria diet in relation to the presence of reflux esophagitis, weight loss and macroscopic changes related to the procedures. Methods: Thirty Wistar rats were randomized into three groups, and after the obesity induction period by means of a 28-day cafeteria diet, underwent a simulated surgery (CG), VG and VGA. The animals were followed up for 28 days in the post-operative period, and after euthanasia, the reflux esophagitis evaluation was histopathologically performed. Weight and macroscopy were the other variables; weight was measured weekly and the macroscopic evaluation was performed during euthanasia. Results: All animals presented some degree of inflammation and the presence of at least one inflammation criterion; however, there was no statistically significant difference in the analysis among the groups. In relation to weight loss, the animals in CG showed a gradual increase during the whole experiment, evolving to super-obesity at the end of the study, while the ones with VG and EVG had weight regain after the first post-operative period; however, a less marked regain compared to CG, both for VG and EVG. Conclusion: There is no difference in relation to reflux esophagitis VG and EVG, as well as macroscopic alterations, and both techniques have the ability to control the evolution of weight during postoperative period in relation to CG.

Highlights • An uncommon syndrome of gastrointestinal obstruction due to vascular compression is presented. • Treatment of Dunbar syndrome by laparoscopy is proposal. • Better results with laparoscopic treatment are discussed. • Others forms of treatment are discussed., Background Dunbar syndrome is a rare disorder due to external compression of the celiac trunk by the median arcuate ligament. The symptoms include abdominal pain, nausea and vomiting. Laparoscopic treatment was suggested as a safe procedure. The aim of this study is to present a case of Dunbar syndrome underwent laparoscopic treatment. Casepresentation A 17-year-old female patient presented at emergency room with upper abdominal pain and dyspepsia, related to food intake. A selective arteriography of the celiac trunk revealed stenosis due to compression of the celiac artery. The decompression of the celiac trunk by the median arcuate ligament was performed. Postoperative course was uneventful and the patient was discharged on the 5th postoperative Conclusions Laparoscopic division of the arcuate ligament in patients with Dunbar syndrome is feasible and safe. This procedure can be performed uneventful in order to restore quality of life of the patient.

Highlights • The options of treatment for caudate tumors are presented. • Treatment of caudate tumors by left-sided approach is proposal. • Better results with left-sided approach are discussed. • Selection of patients with tumors in caudate lobe for left-sided approach is important., Introduction The caudate lobe is a distinct liver lobe and surgical resection requires expertise and precise anatomic knowledge. Left-sided approach was described for resection of small tumors originated in the Spiegel lobe but now the procedure has been performed even for tumors more than five centimeters. The aim of this study is to present three cases of tumor of caudate lobe underwent isolated lobectomy by left-sided approach. Presentation of case Three patients with metastasis of colorectal cancer, carcinoma hepatocellular and metastasis of neuroendocrine tumor underwent resection. After modified Makuuchi incision, early control of short hepatic e short portal veins before hepatectomy was performed. The operative time was 200, 270 and 230 min respectively. No blood transfusion was used and no postoperative complications were observed. The length of stay was 7, 11 and 5 days respectively. Discussion Some approaches have been described to access and resect tumors of the caudate lobe, including the left-sided approach, right-sided approach, combined left- and right-sided approach and the anterior transhepatic approach. For liver resection in patients with malignant disease, parenchymal preservation is important in order to avoid postoperative liver failure or due to the risk of second hepatectomy. In these patients isolated caudate lobectomy is a safe option. Conclusion Isolated caudate lobectomy is a feasible procedure. Left-sided approach can be preformed even for tumors larger than 5 cm.

To determine the opinion and knowledge of the population of Curitiba about organ donation and transplantation.The opinion and knowledge about organ donation and transplantation of 1,000 subjects over 18 years of age were determined. The subjects responded to a questionnaire of 20 queries. Respondents had age, gender, social-economic, and education distributions similar to those of the Brazilian population, as defined by the Brazilian Institute of Geography and Statistics (IBGE).Eighty-seven percent of respondents were in favor of organ donation. There was no difference in the percentage of respondents in favor of donation in relation to gender, marital status, religion, and income. The main reasons in favor of donation were to save life, to help other persons and to donate life. The main reasons against donation were distrust towards medicine or the Brazilian transplantation organization, the existence of organ sale, and fear of body mutilation. Most respondents believed that wealthy people have a better chance to receive an organ than poor people, that sales of organs exists in Brazil, and that misdiagnosis of brain death may occur.Most respondents are in favor of organ donation and have a good knowledge of organ donation and transplantation. The majority distrusts Brazilian organization of organ distribution and brain death diagnosis.