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In this study, mortality effects of BGN-1 which is local diatomaceous earths, were investigated against adults of German cockroach (Blatella gemanica (L.)) on concrete, ceramic floor tile and laminate flooring. On these three different surfaces, B. germanica adults were exposed to BGN-1 diatomaceous earth at the doses of 2.5, 5, 10, 20 g/m2 along 6 days. In all surface applications of BGN-1 diatomaceous earth, exposure time and dose caused significant effect on mortality rates of B. germanica adults. It was determined that BGN-1 coded Turkish diatomaceous earth has the lowest mortality effect on all application surfaces at the dose of 2.5 g/m2. 2.5 g/m2 BGN-1 caused 100 % mortality after 6 days concrete surface and caused 100 % mortality at the end of the fourth day on ceramic floor tile and laminate flooring. On the other hand, doses of 5 and 10 g/m2 of BGN-1 caused 100% B. germanica mortality on all surfaces at the end of the second day, while the highest dose of 20 g /m2 of BGN-1 reached 100% B. germanica mortality at the end of the first day on all application surfaces. In general, the mortality activity of BGN-1 diatomites against B. germanica adults was found to be similar on all three surfaces. At the end of this study, local diatomaceous earth coded BGN-1 was found to be good alternatives for controlling B. germanica which is a medical pest insect.

Histomoniasis was detected and described in naturally affected 35 turkeys. A polymerase chain reaction, which amplified a 209 bp region from the small subunit ribosomal RNA gene of H. meleagridis, was used and compared to the detection by histopathological observation of the histomonad trophozoits. In gross examination, typical histomoniasis lesions of hyperaemia and thickening of the cecal wall with grayish-yellow cecal cores were seen. In livers and kidneys, variably sized multiple foci of circumscribed necrotic areas were observed. Microscopically, varying amounts of histomonad trophozoits were detected in cecum (100%), liver (91.4%), kidney (17.1%), spleen (11.4%), proventriculus (5.7%) and bursa of Fabricius (2.8%). Mononuclear cell infiltration often containing giant cells in ceca, livers, kidneys and a proventriculus was observed. Lymphoid depletion was present in spleen and bursa of Fabricius. PCR assay detected the agent in cecum (100%), liver (100%), kidney (31.4%), spleen (25.7%), proventriculus (11.4%), bursa of Fabricius (11.4%) and lung (17.1%). This study describes histomoniasis lesions especially in several unusual locations such as kidney, spleen, bursa of Fabricius and proventriculus and could provide a help in the diagnosis of the natural histomoniasis in turkeys. It was also shown that PCR based detection was a more sensitive technique than detection based on histopathological observation.

The efficacy of melatonin co-administration on aflatoxicosis in chicks was investigated. Ross PM3 breed chicks were divided into groups of 10 and given conventional feed. One of the groups was kept as a control (C), and the others were given 150ppb aflatoxin (AF1), 300ppb aflatoxin (AF2), 150ppb aflatoxin plus 10mg/kg/bwt melatonin (AF1+M), 300ppb aflatoxin plus 10mg/kg/bwt melatonin (AF2+M), 10mg/kg/bwt melatonin (M), and 1% ethanol (E). After 21 day-treatment period, the chicks were sacrificed, liver and kidney tissues were collected, processed for immuno-histochemical staining, in situ TUNEL method, and biochemical analyses. Vacuolar degeneration, necrosis, bile duct hyperplasia in liver, and mild tubular degeneration in kidney were detected in AF groups. Pathological changes were markedly reduced in AF+M groups, and a microscopic view similar to group C was observed. Increased immunoreactivity against inducible nitric oxide synthase (iNOS) and nitrotyrosine was detected in AF groups compared to weak immunoreactivity in group C. Immunoreactivity in AF+M groups was markedly reduced compared to AF groups and was similar to group C in liver and kidney. Many apoptotic cells were detected in the livers of AF groups, whereas there were no apoptotic cells in AF+M groups. While reduced glutathione (GSH) levels in liver and kidney of AF groups were greatly reduced, malondialdehyde (MDA) levels increased. With melatonin co-administration, the levels of GSH and MDA approached to the values of group C. These results indicated that nitrosative tissue degeneration caused by aflatoxin could be greatly reduced by melatonin supplementation in chicks.

Congenital malformations with multiple anomalies have been described infrequently in the veterinary literature. A stillborn male crossbred lamb with diprosopus, craniorachischisis, and arthrogryposis was examined macroscopically and histopathologically in this study. The left head was smaller than the right head. Micrencephaly, agnathia, and a rudimentary tongue, which was adherent to the palate, were present in the left head. Micrencephaly, brachygnathia superior, and cleft palate were present in the right head. Cerebellar agenesis and spinal cord hypoplasia were observed. The cerebrums and the spinal cord were covered with a tapering membranous structure. Neural and dermal tissues were noted to intervene upon microscopic examination of this structure. Disorganization of neurons was observed in both cerebrums, though it was more severe in the left one. This case demonstrates many congenital defects occurring together in a lamb.