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Many anthropological studies have examined the functional implications of enamel thickness in human dental crowns. Despite limitations, Enamel thickness (ET) values are still used to infer taxonomic attribution in the genus Homo, and to identify mechanisms of functional adaptation against macrowear. However, only a few studies have tried to describe the possible relationship between ET and dental wear patterns in permanent lower first molars (M) aiming to observe whether an adaptive response to the environmental and cultural context is detectable. The present work aims to investigate a possible signal of ET adaptive response in M (wear stage 3; Molnar, 1971) belonging to individuals who lived between the Neolithic (early 6th millennium BCE) and the Bronze Age (second half of the 2nd millennium BCE) in Croatia to identify any signal of change in dental tissue proportions based on archaeologically documented shifts in population structure and subsistence strategies. In order to do so, we explored 3D Average Enamel Thickness (AET) of the entire crown and wear pattern distribution among individuals and across chronological groups. We then described a new method called “Enamel Thickness per Masticatory Phases” (ETMP) involving the creation of virtual sections cutting enamel and coronal dentine in three parts based on masticatory phases, and explored the distribution of 3D AET accordingly. Finally, we performed geometric morphometric analysis on dental crown to ascertain possible morphological differences between Neolithic, Eneolithic, and Bronze Age groups. Results show that Bronze Age individuals differ from previous groups due to 1) higher values of ET in both the entire crown and specifically in the buccal area, 2) to an extensive wear pattern localized on the buccal side, and 3) to the distal extension of the hypoconid together with an extended mesio-distal shape of the crown. These patterns may represent an adaptive response of dental tissue to varying functional de

Modern humans have populated Europe for more than 45,000 years1,2. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe4, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants.

Modern humans have populated Europe for more than 45,000 years. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants.

The pile dwelling, which has been only limitedly excavated in 1999, is located along the old watercourse of the river Tartaro in the Verona plain. The dwelling can be dated to the Early Bronze Age and the materials belong to the Polada culture with affinities with the middle Danubian culture of Wieselburg-Gàta. The excavations brought to light a complete neurocranium and fragmentary remains of the skeletal face of a child about 6 years old immediately above at inhabited levels. From these, 10 bone fragments of cranial vault probably belonging to an adult and child were found. Dendrochronological investigation allowed the absolute dating of the wooden structures in the archeological area, thanks to the cross-dating against the oak chronology GARDA 1. Felling dates goes from 1963-1943 to 1933-1915 BC. The analysis of the faunal remains, the carpological remains and some wooden artifacts completes the cognitive framework on the economy and the exploitation of wood in the phases of life of the settlement.

We present the results of a multi-disciplinary investigation on a deciduous human tooth (Pradis 1), recently recovered from the Epigravettian layers of the Grotte di Pradis archaeological site (Northeastern Italian Prealps). Pradis 1 is an exfoliated deciduous molar (Rdm2), lost during life by an 11—12-year-old child. A direct radiocarbon date provided an age of 13,088—12,897 cal BP (95% probability, IntCal20). Amelogenin peptides extracted from tooth enamel and analysed through LC¿MS/MS indicate that Pradis 1 likely belonged to a male. Time-resolved 87Sr/86Sr analyses by laser ablation mass spectrometry (LA-MC-ICPMS), combined with dental histology, were able to resolve his movements during the first year of life (i.e. the enamel mineralization interval). Specifically, the Sr isotope ratio of the tooth enamel differs from the local baseline value, suggesting that the child likely spent his first year of life far from Grotte di Pradis. Sr isotopes are also suggestive of a cyclical/seasonal mobility pattern exploited by the Epigravettian human group. The exploitation of Grotte di Pradis on a seasonal, i.e. summer, basis is also indicated by the faunal spectra. Indeed, the nearly 100% occurrence of marmot remains in the entire archaeozoological collection indicates the use of Pradis as a specialized marmot hunting or butchering site. This work represents the first direct assessment of sub-annual movements observed in an Epigravettian hunter-gatherer group from Northern Italy.

[Objectives] During the middle-to-upper Paleolithic transition (50,000 and 40,000¿years ago), interaction between Neanderthals and Homo sapiens varied across Europe. In southern Italy, the association between Homo sapiens fossils and non-Mousterian material culture, as well as the mode and tempo of Neanderthal demise, are still vividly debated. In this research, we focus on the study of two human teeth by using 3D geometric morphometric approaches for a reliable taxonomical attribution as well as obtaining new radiometric dates on the archeological sequence., [Material and Methods] This work presents two lower deciduous molars uncovered at Roccia San Sebastiano (Mondragone-Caserta, Italy), stratigraphically associated with Mousterian (RSS1) and Uluzzian (RSS2) artifacts. To obtain a probabilistic attribution of the two RSS teeth to each reference taxa group composed of Neanderthals and Homo sapiens, we performed and compared the performance of three supervised learning algorithms (flexible discriminant analysis, multiadaptive regression splines, and random forest) on both crown and cervical outlines obtained by virtual morphometric methods. [Discussion] This site yields the most recent direct evidence for a Neanderthal presence in southern Italy and confirms a later shift to upper Paleolithic technology in southwestern Italy compared to the earliest Uluzzian evidence at Grotta del Cavallo (Puglia, Italy).

The reconstruction of the original morphology of bones and teeth after sampling for physicochemical (e.g., radiocarbon and uranium series dating, stable isotope analysis, paleohistology, trace element analysis) and biomolecular analyses (e.g., ancient DNA, paleoproteomics) is appropriate in many contexts and compulsory when dealing with fossil human remains. The reconstruction protocols available to date are mostly based on manual re-integration of removed portions and can lead to an imprecise recovery of the original morphology. In this work, to restore the original external morphology of sampled teeth we used computed microtomography (microCT), reverse engineering (RE), computer-aided design (CAD) and rapid prototyping (RP) techniques to fabricate customized missing parts. The protocol was tested by performing the reconstruction of two Upper Palaeolithic human teeth from the archaeological excavations of Roccia San Sebastiano (Mondragone, Caserta, southern Italy) and Riparo I of Grotte Verdi di Pradis (Clauzetto, Pordenone, north-eastern Italy) (RSS2 and Pradis 1, respectively), which were sampled for physicochemical and biomolecular analyses. It involved a composite procedure consisting in: a) the microCT scanning of the original specimens; b) sampling; c) the microCT scanning of the specimens after sampling; d) the reconstruction of the digital 3D surfaces of the specimens before and after sampling; e) the creation of digital models of the missing/sampled portions by subtracting the 3D images of the preserved portions (after the sampling) from the images of the intact specimens (before the sampling) by using reverse engineering techniques; f) the prototyping of the missing/sampled portions to be integrated; g) the painting and application of the prototypes through the use of compatible and reversible adhesives. By following the proposed protocol, in addition to the fabrication of a physical element which is faithful to the original, it was possible to obtain a r

During the Middle to Upper Palaeolithic transition (50,000 and 40,000 years ago), interaction between Neanderthals and Homo sapiens varied across Europe. In southern Italy, the association between Homo sapiens fossils and non-Mousterian material culture, as well as the mode and tempo of Neanderthal demise, are still vividly debated. This work presents two lower deciduous molars uncovered at Roccia San Sebastiano (Mondragone-Caserta, Italy), stratigraphically associated with Mousterian (RSS1) and Uluzzian (RSS2) artefacts. Using virtual morphometric methods and supervised learning algorithms we show that RSS1, whose Mousterian context appears more recent than 44,800-44,230 cal BP, can be attributed to a Neanderthal, while RSS2, found in an Uluzzian context that we dated to 42,640-42,380 cal BP, is attributed to Homo sapiens. This site therefore yields the most recent direct evidence for a Neanderthal presence in southern Italy and confirms a later shift to Early Upper Palaeolithic technology in southwestern Italy compared to the earliest Uluzzian evidence at Grotta del Cavallo (Puglia, Italy).

During the Middle to Upper Palaeolithic transition (50,000 and 40,000 years ago), interaction between Neanderthals and Homo sapiens varied across Europe. In southern Italy, the association between Homo sapiens fossils and non-Mousterian material culture, as well as the mode and tempo of Neanderthal demise, are still vividly debated. This work presents two lower deciduous molars uncovered at Roccia San Sebastiano (Mondragone-Caserta, Italy), stratigraphically associated with Mousterian (RSS1) and Uluzzian (RSS2) artefacts. Using virtual morphometric methods and supervised learning algorithms we show that RSS1, whose Mousterian context appears more recent than 44,800-44,230 cal BP, can be attributed to a Neanderthal, while RSS2, found in an Uluzzian context that we dated to 42,640-42,380 cal BP, is attributed to Homo sapiens. This site therefore yields the most recent direct evidence for a Neanderthal presence in southern Italy and confirms a later shift to Early Upper Palaeolithic technology in southwestern Italy compared to the earliest Uluzzian evidence at Grotta del Cavallo (Puglia, Italy).

Neanderthal extinction has been a matter of debate for many years. New discoveries, better chronologies and genomic evidence have done much to clarify some of the issues. This evidence suggests that Neanderthals became extinct around 40,000-37,000 years before present (BP), after a period of coexistence with Homo sapiens of several millennia, involving biological and cultural interactions between the two groups. However, the bulk of this evidence relates to Western Eurasia, and recent work in Central Asia and Siberia has shown that there is considerable local variation. Southwestern Asia, despite having a number of significant Neanderthal remains, has not played a major part in the debate over extinction. Here we report a Neanderthal deciduous canine from the site of Bawa Yawan in the West-Central Zagros Mountains of Iran. The tooth is associated with Zagros Mousterian lithics, and its context is preliminary dated to between ~43,600 and ~41,500 years ago.

Objectives Palate morphology is constantly changing throughout an individual's lifespan, yet its asymmetry during growth is still little understood. In this research, we focus on the study of palate morphology by using 3D geometric morphometric approaches to observe changes at different stages of life, and to quantify the impact of directional and fluctuating asymmetry on different areas at different growth stages.Materials and Methods The sample consists of 183 individuals (1-72 years) from two identified human skeletal collections of 19th and early 20th Century Italian contexts. A 3D-template of 41 (semi)landmarks was applied on digital palate models to observe morphological variation during growth.Results Asymmetrical components of the morphological structure appears multidirectional on the entire palate surface in individualsDiscussion Morphological structure appears instable during the first year of life and acquires an opposite asymmetric bilateral direction between 2 and 6 years of age. This condition has been also documented in adults; when paired with vertical alteration, anterior/posterior asymmetry seems to characterize palate morphology, which is probably due to mechanical factors during the lifespan. Fluctuating asymmetry is predominant in the first period of life due to a plausible relationship with the strength of morphological instability of the masticatory system. Directional asymmetry, on the other hand, shows that the patterning of group-level morphological change might be explained as a functional response to differential inputs (physiological forces, nutritive and non-nutritive habits, para-masticatory activity as well as the development of speech) in different growth stages. This research has implications with respect to medical and evolutionary fields. In medicine, palate morphology should be considered when planning orthodontic and surgical procedures as it could affect the outcome. As far as an evolutionary perspectiv

The present digital archive is the outcome of the paper: Oxilia, G., Menghi Sartorio JC., Bortolini, E. et al. Exploring Directional and Fluctuating Asymmetry in the human palate during growth. The american Journal of Physical Anthropology. The dataset included in this repository provides a collection of palatal arches of 183 individuals from two Italian identified human skeletal collections (Bologna and Florence) aged from 1 to 72 years. The individuals from the Bologna collection (n=87), housed at the Museum of Anthropology of the University of Bologna, are from the Certosa cemetery (Bologna). The collection includes a total of 425 individuals of known sex, name and age at death (range 0-91 years), most of whom belonged to the less-advantaged urban classes of late 19th and early 20th Century (Belcastro et al. 2017). The sample from the Florence collection (n=96), hosted in the Natural History Museum (Anthropology and Ethnology section), University of Florence, belongs to unclaimed indigents from the Florence hospital and comprises lower-class citizens of known sex, name and age at death (range 1-57 years), who lived in the town of Florence in the 19th century (industrialized only after 1890). Paleopathological information of the Bologna individuals was sourced from archival data, which indicated that most of the individuals died following infections diseases (Belcastro et al. 2017) while the Florence individuals health profiles indicated a tendency to breastfeed children until 12-18 months of age and an unbalanced dietary intake induced by poor living conditions (Moggi-Cecchi et al., 1994). Both these findings were consistent with pre- and post-unification Italy in Bologna and pre-industrialised Florence. For the study, male and female individuals from Bologna and Florence were separated into six groups (Table 1) based on direct observation of skeletal remains. Age groups were subdivided considering mixed dentition until permanent dentition based on the time of er