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19. Sperm morphology, motility, and concentration in fertile and infertile men

Author

Guzick, David S., Overstreet, James W., Factor-Litvak, Pam, Brazil, Charlene K., Nakajima, Steven T., Coutifaris, Christos, Carson, Sandra Ann, Cisneros, Pauline, Steinkampf, Michael P., Hill, Joseph A., Xu, Dong, and Vogel, Donna L.

Subjects
Infertility, Male -- Diagnosis, Spermatozoa -- Motility
Abstract

It may be very difficult to diagnose male infertility by analyzing the number, movement, or shape of sperm. In a study of 765 infertile men and 696 fertile men, sperm characteristics varied considerably and there was a substantial overlap in sperm quality between both groups.

Published
2001

20. Efficacy of superovulation and intrauterine insemination in the treatment of infertility

Author

Guzick, David S., Carson, Sandra Ann, Coutifaris, Christos, Overstreet, James W., Factor-Litvak, Pam, Steinkampf, Michael P., Hill, Joseph A., Mastroianni, Luigi, Jr., Buster, John E., Nakajima, Steven T., Vogel, Donna L., and Canfield, Robert E.

Subjects
Infertility, Female -- Care and treatment, Artificial insemination, Human -- Evaluation
Abstract

Superovulation and intrauterine insemination appears to be more effective in the treatment of infertility than many other methods. In this technique, the woman is given hormones to stimulate ovulation and then the man's sperm is injected into the uterus through the cervix. Researchers compared the pregnancy rates in 231 couples treated with this technique to the rates in 234 couples who received intrauterine insemination alone and two other groups of women who received their partner's sperm using other methods, with or without superovulation. Pregnancy rates were highest in the women who received superovulation and intrauterine insemination.

Published
1999

26. The PH-20 protein in cynomolgus macaque spermatozoa: identification of two different forms exhibiting hyaluronidase activity

Author

Cherr, Gary N., Meyers, Stuart A., Yudin, Ashley I., VandeVoort, Catherine A., Myles, Diana G., Primakoff, Paul, and Overstreet, James W.

Subjects
Spermatozoa -- Research, Proteins -- Research, Macaques -- Research, Enzymes -- Regulation, Biological sciences
Abstract

In these experiments, we have characterized the bifunctional sperm protein PH-20 in macaque sperm and studied its hyaluronidase activity. Intact sperm were evaluated before the acrosome reaction (AR), and a soluble form of PH-20 released during acrosomal exocytosis was also investigated. Western blots of SDS-PAGE of acrosome-intact sperm extracts revealed a 64-kDa form of PH-20 was recognized by a polyclonal antibody (R-10) raised in rabbits against purified, recombinant cynomolgus macaque sperm PH-20. The soluble components released during the AR which were recognized by the R-10 antibody included both the 64-kDa form and a 53-kDa form of PH-20. An ELISA-like procedure for determining PH-20 hyaluronidase activity indicated that acrosome-intact sperm exhibited two peaks of hyaluronidase activity near pH 4 and [greater than or equal to]pH 7. The majority of enzyme activity in acrosome-intact sperm extracts occurred at neutral pH, while the soluble hyaluronidase activity released at the AR was predominantly acid-active. Hyaluronidase activity of PH-20 at different pH optima was investigated using hyaluronic acid substrate gel electrophoresis, and results indicated that the 64-kDa polypeptide had a broad range, with the majority of activity at neutral pH (pH 7). The 53-kDa polypeptide in sperm extracts only exhibited activity at acid pH (pH 4). The hyaluronidase activities of both enzymes could be inhibited by apigenin. The soluble PH-20 hyaluronidase activity released during the AR was primarily of the acid-active 53-kDa form. Fine structural localization of PH-20 using Fab fragments of R-10 IgG demonstrated that PH-20 was associated not only with sperm membranes, but also with the dispersing acrosomal contents. These data suggest that the more neutral-active form of PH-20 (64 kDa) is present on the plasma and inner acrosomal membranes and gives rise to the soluble acid-active form at the time of the AR. The generation of the soluble form of PH-20 may result from the action of acrosomal enzymes, which could include proteases, glycosidases, and phospholipases.

Published
1996

39. The Authors Reply

Author

Schenker, Marc B., Samuels, Steven, Eaton, Muzza, and Overstreet, James W.

Published
1987

41. A Common Mutation in the Defensin DEFB126 Causes Impaired Sperm Function and Subfertility

Author

Tollner, Theodore L., primary, Venners, Scott A., additional, Hollox, Edward J., additional, Yudin, Ashley I., additional, Liu, Xue, additional, Tang, Genfu, additional, Xing, Houxun, additional, Kays, Robert J., additional, Lau, Tsang, additional, Overstreet, James W., additional, Xu, Xiping, additional, Bevins, Charles L., additional, and Cherr, Gary N., additional

Published
2011
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43. Serum inhibin-b in fertile men is strongly correlated with low but not high sperm counts: a coordinated study of 1,797 European and US men

Author

Jørgensen, Niels, primary, Liu, Fan, additional, Andersson, Anna-Maria, additional, Vierula, Matti, additional, Irvine, D. Stewart, additional, Auger, Jacques, additional, Brazil, Charlene K., additional, Drobnis, Erma Z., additional, Jensen, Tina K., additional, Jouannet, Pierre, additional, Overstreet, James W., additional, Redmon, J. Bruce, additional, Sparks, Amy, additional, Toppari, Jorma, additional, Wang, Christina, additional, Skakkebæk, Niels E., additional, and Swan, Shanna H., additional

Published
2010
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