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20 results on '"Overmeer, René M"'

3. Sequential cancer mutations in cultured human intestinal stem cells

Author

Drost, Jarno, van Jaarsveld, Richard H., Ponsioen, Bas, Zimberlin, Cheryl, van Boxtel, Ruben, Buijs, Arjan, Sachs, Norman, Overmeer, René M., Offerhaus, Johan G., Begthel, Harry, Korving, Jeroen, van de Wetering, Marc, Schwank, Gerald, Logtenberg, Meike, Cuppen, Edwin, Snippert, Hugo J., Paul Medema, Jan, Kops, Geert J. P. L., and Clevers, Hans

Published
2015
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8. Live imaging of cell division in 3D stem-cell organoid cultures

Author

Bolhaqueiro, Ana C F, van Jaarsveld, Richard H, Ponsioen, Bas, Overmeer, René M, Snippert, Hugo J, Kops, Geert J P L, and Hubrecht Institute for Developmental Biology and Stem Cell Research

Subjects
Stem Cells/physiology, Animals, Cell Culture Techniques/methods, Cell Division/physiology, Humans, Organoids/physiology, Microscopy/methods
Abstract

Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids.

Published
2018

9. Live imaging of cell division in 3D stem-cell organoid cultures

Author

Bolhaqueiro, Ana C.F., van Jaarsveld, Richard H., Ponsioen, Bas, Overmeer, René M., Snippert, Hugo J., Kops, Geert J.P.L., Bolhaqueiro, Ana C.F., van Jaarsveld, Richard H., Ponsioen, Bas, Overmeer, René M., Snippert, Hugo J., and Kops, Geert J.P.L.

Published
2018

10. Live imaging of cell division in 3D stem-cell organoid cultures

Author

Cancer, CMM Groep Snippert, CMM Sectie Molecular Cancer Research, CMM USEQ Facility, Bolhaqueiro, Ana C.F., van Jaarsveld, Richard H., Ponsioen, Bas, Overmeer, René M., Snippert, Hugo J., Kops, Geert J.P.L., Cancer, CMM Groep Snippert, CMM Sectie Molecular Cancer Research, CMM USEQ Facility, Bolhaqueiro, Ana C.F., van Jaarsveld, Richard H., Ponsioen, Bas, Overmeer, René M., Snippert, Hugo J., and Kops, Geert J.P.L.

Published
2018

11. Corrigendum: Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors

Author

Steinhart, Zachary, Pavlovic, Zvezdan, Chandrashekhar, Megha, Hart, Traver, Wang, Xiaowei, Zhang, Xiaoyu, Robitaille, Mélanie, Brown, Kevin R, Jaksani, Sridevi, Overmeer, René M, Boj, Sylvia F, Adams, Jarrett, Pan, James, Clevers, Hans, Sidhu, Sachdev, Moffat, Jason, Angers, Stéphane, Steinhart, Zachary, Pavlovic, Zvezdan, Chandrashekhar, Megha, Hart, Traver, Wang, Xiaowei, Zhang, Xiaoyu, Robitaille, Mélanie, Brown, Kevin R, Jaksani, Sridevi, Overmeer, René M, Boj, Sylvia F, Adams, Jarrett, Pan, James, Clevers, Hans, Sidhu, Sachdev, Moffat, Jason, and Angers, Stéphane

Abstract

This corrects the article DOI: 10.1038/nm.4219.

Published
2017

12. Targeting mutant RAS in patient-derived colorectal cancer organoids by combinatorial drug screening

Author

Verissimo, Carla S, Overmeer, René M, Ponsioen, Bas, Drost, Jarno, Mertens, Sander, Verlaan-Klink, Ingrid, Gerwen, Bastiaan van, van der Ven, Marieke, van de Wetering, Marc, Egan, David A, Bernards, René, Clevers, Hans, Bos, Johannes L, Snippert, Hugo J, Verissimo, Carla S, Overmeer, René M, Ponsioen, Bas, Drost, Jarno, Mertens, Sander, Verlaan-Klink, Ingrid, Gerwen, Bastiaan van, van der Ven, Marieke, van de Wetering, Marc, Egan, David A, Bernards, René, Clevers, Hans, Bos, Johannes L, and Snippert, Hugo J

Published
2016

13. Targeting mutant RAS in patient-derived colorectal cancer organoids by combinatorial drug screening

Author

CMM Groep Bos, Cancer, CMM Groep Snippert, Hubrecht Institute with UMC, CMM Cell Screening Center, CMM Sectie Molecular Cancer Research, Regenerative Medicine and Stem Cells, Child Health, Verissimo, Carla S, Overmeer, René M, Ponsioen, Bas, Drost, Jarno, Mertens, Sander, Verlaan-Klink, Ingrid, Gerwen, Bastiaan van, van der Ven, Marieke, van de Wetering, Marc, Egan, David A, Bernards, René, Clevers, Hans, Bos, Johannes L, Snippert, Hugo J, CMM Groep Bos, Cancer, CMM Groep Snippert, Hubrecht Institute with UMC, CMM Cell Screening Center, CMM Sectie Molecular Cancer Research, Regenerative Medicine and Stem Cells, Child Health, Verissimo, Carla S, Overmeer, René M, Ponsioen, Bas, Drost, Jarno, Mertens, Sander, Verlaan-Klink, Ingrid, Gerwen, Bastiaan van, van der Ven, Marieke, van de Wetering, Marc, Egan, David A, Bernards, René, Clevers, Hans, Bos, Johannes L, and Snippert, Hugo J

Published
2016

14. Targeting mutant RAS in patient-derived colorectal cancer organoids by combinatorial drug screening

Author

Verissimo, Carla S, primary, Overmeer, René M, additional, Ponsioen, Bas, additional, Drost, Jarno, additional, Mertens, Sander, additional, Verlaan-Klink, Ingrid, additional, Gerwen, Bastiaan van, additional, van der Ven, Marieke, additional, Wetering, Marc van de, additional, Egan, David A, additional, Bernards, René, additional, Clevers, Hans, additional, Bos, Johannes L, additional, and Snippert, Hugo J, additional

Published
2016
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15. Author response: Targeting mutant RAS in patient-derived colorectal cancer organoids by combinatorial drug screening

Author

Verissimo, Carla S, primary, Overmeer, René M, additional, Ponsioen, Bas, additional, Drost, Jarno, additional, Mertens, Sander, additional, Verlaan-Klink, Ingrid, additional, Gerwen, Bastiaan van, additional, van der Ven, Marieke, additional, Wetering, Marc van de, additional, Egan, David A, additional, Bernards, René, additional, Clevers, Hans, additional, Bos, Johannes L, additional, and Snippert, Hugo J, additional

Published
2016
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19. Live imaging of cell division in 3D stem-cell organoid cultures.

Author

Bolhaqueiro ACF, van Jaarsveld RH, Ponsioen B, Overmeer RM, Snippert HJ, and Kops GJPL

Subjects
Animals, Cell Culture Techniques methods, Humans, Cell Division physiology, Microscopy methods, Organoids physiology, Stem Cells physiology
Abstract

Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids., (© 2018 Elsevier Inc. All rights reserved.)

Published
2018
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20. Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment.

Author

Overmeer RM, Gourdin AM, Giglia-Mari A, Kool H, Houtsmuller AB, Siegal G, Fousteri MI, Mullenders LH, and Vermeulen W

Subjects
Binding Sites, Cell Line, Cytarabine pharmacology, DNA Damage, DNA Replication, Fluorescence Recovery After Photobleaching, Gene Knockdown Techniques, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Humans, Hydroxyurea pharmacology, Kinetics, Models, Biological, Nucleic Acid Synthesis Inhibitors pharmacology, Proliferating Cell Nuclear Antigen metabolism, RNA, Small Interfering genetics, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Replication Protein C antagonists & inhibitors, Replication Protein C genetics, Ultraviolet Rays, DNA Polymerase III metabolism, DNA Repair physiology, Replication Protein C metabolism
Abstract

Nucleotide excision repair (NER) operates through coordinated assembly of repair factors into pre- and postincision complexes. The postincision step of NER includes gap-filling DNA synthesis and ligation. However, the exact composition of this NER-associated DNA synthesis complex in vivo and the dynamic interactions of the factors involved are not well understood. Using immunofluorescence, chromatin immunoprecipitation, and live-cell protein dynamic studies, we show that replication factor C (RFC) is implicated in postincision NER in mammalian cells. Small interfering RNA-mediated knockdown of RFC impairs upstream removal of UV lesions and abrogates the downstream recruitment of DNA polymerase delta. Unexpectedly, RFC appears dispensable for PCNA recruitment yet is required for the subsequent recruitment of DNA polymerases to PCNA, indicating that RFC is essential to stably load the polymerase clamp to start DNA repair synthesis at 3' termini. The kinetic studies are consistent with a model in which RFC exchanges dynamically at sites of repair. However, its persistent localization at stalled NER complexes suggests that RFC remains targeted to the repair complex even after loading of PCNA. We speculate that RFC associates with the downstream 5' phosphate after loading; such interaction would prevent possible signaling events initiated by the RFC-like Rad17 and may assist in unloading of PCNA.

Published
2010
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