Search

Your search keyword '"Ouyang, Yingshi"' showing total 42 results
Start Over Author "Ouyang, Yingshi"
42 results on '"Ouyang, Yingshi"'

7. High-yield and rapid isolation of extracellular vesicles by flocculation via orbital acoustic trapping: FLOAT

Author

Rufo, Joseph, primary, Zhang, Peiran, additional, Wang, Zeyu, additional, Gu, Yuyang, additional, Yang, Kaichun, additional, Rich, Joseph, additional, Chen, Chuyi, additional, Zhong, Ruoyu, additional, Jin, Ke, additional, He, Ye, additional, Xia, Jianping, additional, Li, Ke, additional, Wu, Jiarong, additional, Ouyang, Yingshi, additional, Sadovsky, Yoel, additional, Lee, Luke P., additional, and Huang, Tony Jun, additional

Published
2024
Full Text
View/download PDF

13. The lipase cofactor CGI58 controls placental lipolysis

Author

Guerrero-Santoro, Jennifer, primary, Morizane, Mayumi, additional, Oh, Soo-Young, additional, Mishima, Takuya, additional, Goff, Julie P., additional, Bildirici, Ibrahim, additional, Sadovsky, Elena, additional, Ouyang, Yingshi, additional, Tyurin, Vladimir A., additional, Tyurina, Yulia Y., additional, Kagan, Valerian E., additional, and Sadovsky, Yoel, additional

Published
2023
Full Text
View/download PDF

19. Placental response to maternal SARS-CoV-2 infection

Author

Mourad, Mirella, primary, Jacob, Taylor, additional, Sadovsky, Elena, additional, Bejerano, Shai, additional, Simone, Glicella Salazar-De, additional, Bagalkot, Tarique Rajasaheb, additional, Zucker, Jason, additional, Yin, Michael T., additional, Chang, Jennifer Y., additional, Liu, Lihong, additional, Debelenko, Larisa, additional, Shawber, Carrie J., additional, Firestein, Morgan, additional, Ouyang, Yingshi, additional, Gyamfi-Bannerman, Cynthia, additional, Penn, Anna, additional, Sorkin, Alexander, additional, Wapner, Ronald, additional, and Sadovsky, Yoel, additional

Published
2021
Full Text
View/download PDF

21. PLAC-S-19–00704 / Placental Small Extracellular Vesicles: Current Questions and Investigative Opportunities

Author

Sadovsky, Yoel, Ouyang, Yingshi, Powell, Juliana S., Li, Hui, Mouillet, Jean-Francois, Morelli, Adrian E., Sorkin, Alexander, and Margolis, Leonid

Subjects
Pregnancy Complications, Extracellular Vesicles, Pregnancy, Placenta, Animals, Humans, Female, Article
Abstract

The discovery of regulated trafficking of extracellular vesicles (EVs) has added a new dimension to our understanding of local and distant communication among cells and tissues. Notwithstanding the expanded landscape of EV subtypes, the majority of research in the field centers on small and large EVs that are commonly termed exosomes, microvesicles and apoptotic cell-derived vesicles. In the context of pregnancy, EV-based communication has a special role in the crosstalk among the placenta, maternal and fetal compartments, with most studies focusing on trophoblastic EVs and their effect on other placental cell types, endothelial cells, and distant tissues. Many unanswered questions in the field of EV biology center on the mechanisms of vesicle biogenesis, loading of cargo molecules, EV release and trafficking, the interaction of EVs with target cells and the endocytic pathways underlying their uptake, and the intracellular processing of EVs inside target cells. These questions are directly relevant to EV-based placental-maternal-fetal communication and have unique implications in the context of interaction between two organisms. Despite rapid progress in the field, the number of speculative, unsubstantiated assumptions about placental EVs is concerning. Here we attempt to delineate existing knowledge in the field, focusing primarily on placental small EVs (exosomes). We define central questions that require investigative attention in order to advance the field.

Published
2020

22. Polo inhibits progenitor self-renewal and regulates Numb asymmetry by phosphorylating Pon

Author

Wang, Hongyan, Ouyang, Yingshi, Somers, W. Gregory, Chia, William, and Lu, Bingwei

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Hongyan Wang [1, 3]; Yingshi Ouyang [2, 3]; W. Gregory Somers [1]; William Chia (corresponding author) [1]; Bingwei Lu (corresponding author) [2] Self-renewal and differentiation are cardinal features of [...]

Published
2007

24. Ferroptosis induces membrane blebbing in placental trophoblasts

Author

Kajiwara, Kazuhiro, primary, Beharier, Ofer, additional, Chng, Choon-Peng, additional, Goff, Julie P., additional, Ouyang, Yingshi, additional, St Croix, Claudette M., additional, Huang, Changjin, additional, Kagan, Valerian E., additional, Hsia, K. Jimmy, additional, and Sadovsky, Yoel, additional

Published
2021
Full Text
View/download PDF

25. Acoustofluidic centrifuge for nanoparticle enrichment and separation

Author

Gu, Yuyang, primary, Chen, Chuyi, additional, Mao, Zhangming, additional, Bachman, Hunter, additional, Becker, Ryan, additional, Rufo, Joseph, additional, Wang, Zeyu, additional, Zhang, Peiran, additional, Mai, John, additional, Yang, Shujie, additional, Zhang, Jinxin, additional, Zhao, Shuaiguo, additional, Ouyang, Yingshi, additional, Wong, David T. W., additional, Sadovsky, Yoel, additional, and Huang, Tony Jun, additional

Published
2021
Full Text
View/download PDF

32. Trophoblastic extracellular vesicles and viruses: Friends or foes?

Author

Ouyang, Yingshi, Mouillet, Jean‐Francois, Sorkin, Alexander, and Sadovsky, Yoel

Subjects
*EXTRACELLULAR vesicles, *VIRUS diseases, *CELL communication, *VIRUSES, *VIRAL replication
Abstract

Cells produce cytoplasmic vesicles to facilitate the processing and transport of RNAs, proteins, and other signaling molecules among intracellular organelles. Moreover, most cells release a range of extracellular vesicles (EVs) that mediate intercellular communication in both physiological and pathological settings. In addition to a better understanding of their biological functions, the diagnostic and therapeutic prospects of EVs, particularly the nano‐sized small EVs (sEVs, exosomes), are currently being rigorously pursued. While EVs and viruses such as retroviruses might have evolved independently, they share a number of similar characteristics, including biogenesis pathways, size distribution, cargo, and cell‐targeting mechanisms. The interplay of EVs with viruses has profound effects on viral replication and infectivity. Our research indicates that sEVs, produced by primary human trophoblasts, can endow other non‐placental cell types with antiviral response. Better insights into the interaction of EVs with viruses may illuminate new ways to attenuate viral infections during pregnancy, and perhaps develop new antiviral therapeutics to protect the feto‐placental unit during critical times of human development. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

34. Isolation of exosomes from whole blood by integrating acoustics and microfluidics

Author

Massachusetts Institute of Technology. Department of Materials Science and Engineering, Dao, Ming, Wu, Mengxi, Ouyang, Yingshi, Wang, Zeyu, Zhang, Rui, Huang, Po-Hsun, Chen, Chuyi, Li, Hui, Li, Peng, Quinn, David, Suresh, Subra, Sadovsky, Yoel, Huang, Tony Jun, Massachusetts Institute of Technology. Department of Materials Science and Engineering, Dao, Ming, Wu, Mengxi, Ouyang, Yingshi, Wang, Zeyu, Zhang, Rui, Huang, Po-Hsun, Chen, Chuyi, Li, Hui, Li, Peng, Quinn, David, Suresh, Subra, Sadovsky, Yoel, and Huang, Tony Jun

Abstract

Exosomes are nanoscale extracellular vesicles that play an important role in many biological processes, including intercellular communications, antigen presentation, and the transport of proteins, RNA, and other molecules. Recently there has been significant interest in exosome-related fundamental research, seeking new exosome-based biomarkers for health monitoring and disease diagnoses. Here, we report a separation method based on acoustofluidics (i.e., the integration of acoustics and microfluidics) to isolate exosomes directly from whole blood in a label-free and contact-free manner. This acoustofluidic platform consists of two modules: a microscale cell-removal module that first removes larger blood components, followed by extracellular vesicle subgroup separation in the exosome-isolation module. In the cell-removal module, we demonstrate the isolation of 110-nm particles from a mixture of micro- and nanosized particles with a yield greater than 99%. In the exosome-isolation module, we isolate exosomes from an extracellular vesicle mixture with a purity of 98.4%. Integrating the two acoustofluidic modules onto a single chip, we isolated exosomes from whole blood with a blood cell removal rate of over 99.999%. With its ability to perform rapid, biocompatible, label-free, contact-free, and continuous-flow exosome isolation, the integrated acoustofluidic device offers a unique approach to investigate the role of exosomes in the onset and progression of human diseases with potential applications in health monitoring, medical diagnosis, targeted drug delivery, and personalized medicine. Keywords: extracellular vesicles; exosomes; blood-borne vesicles; surface acoustic waves; acoustic tweezers, National Science Foundation (U.S.) (Grant R01 HD086325), National Science Foundation (U.S.) (Grant IIP-1534645)

Published
2018

42. Ferroptosis induces membrane blebbing in placental trophoblasts.

Author

Kajiwara K, Beharier O, Chng CP, Goff JP, Ouyang Y, St Croix CM, Huang C, Kagan VE, Hsia KJ, and Sadovsky Y

Subjects
Female, Humans, Lipid Peroxidation, Phospholipid Hydroperoxide Glutathione Peroxidase, Placenta, Pregnancy, Trophoblasts, Ferroptosis
Abstract

Ferroptosis is a regulated, non-apoptotic form of cell death, characterized by hydroxy-peroxidation of discrete phospholipid hydroperoxides, particularly hydroperoxyl (Hp) forms of arachidonoyl- and adrenoyl-phosphatidylethanolamine, with a downstream cascade of oxidative damage to membrane lipids, proteins and DNA, culminating in cell death. We recently showed that human trophoblasts are particularly sensitive to ferroptosis caused by depletion or inhibition of glutathione peroxidase 4 (GPX4) or the lipase PLA2G6. Here, we show that trophoblastic ferroptosis is accompanied by a dramatic change in the trophoblast plasma membrane, with macro-blebbing and vesiculation. Immunofluorescence revealed that ferroptotic cell-derived blebs stained positive for F-actin, but negative for cytoplasmic organelle markers. Transfer of conditioned medium that contained detached macrovesicles or co-culture of wild-type target cells with blebbing cells did not stimulate ferroptosis in target cells. Molecular modeling showed that the presence of Hp-phosphatidylethanolamine in the cell membrane promoted its cell ability to be stretched. Together, our data establish that membrane macro-blebbing is characteristic of trophoblast ferroptosis and can serve as a useful marker of this process. Whether or not these blebs are physiologically functional remains to be established., Competing Interests: Competing interests Y.S. is a consultant at Illumina, Inc. The other authors report no conflicts., (© 2021. Published by The Company of Biologists Ltd.)

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results