28 results on '"Ouellette T"'
Search Results
2. STIM1 deficiency results in impaired platelet procoagulant activity and protection from arterial thrombosis: O-TH-049
- Author
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Ahmad, F, Stefanini, L, Ouellette, T D, Greene, T, Feske, S, and Bergmeier, W
- Published
- 2011
3. Molybdenum(V) Fluoride (Molybdenum Pentafluoride)
- Author
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Ouellette, T. J., primary, Ratcliffe, C. T., additional, Sharp, D. W. A., additional, Steven, A. M., additional, and Schreiner, F., additional
- Published
- 2007
- Full Text
- View/download PDF
4. Relative contributions of stromal interaction molecule 1 and CalDAG-GEFI to calcium-dependent platelet activation and thrombosis: STIM1 and CalDAG-GEFI in platelet activation
- Author
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AHMAD, F., BERGMEIER, W., PONCZ, M., FESKE, S., BOULAFTALI, Y., GREENE, T. K., and OUELLETTE, T. D.
- Abstract
Stromal interaction molecule 1 (STIM1) was recently identified as a critical component of store-operated calcium entry (SOCE) in platelets. We previously reported the Ca2+-sensing guanine nucleotide exchange factor CalDAG-GEFI as critical molecule in Ca2+ signaling in platelets.
- Published
- 2011
- Full Text
- View/download PDF
5. Application of added beam filtration to intra-oral radiography.
- Author
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Kapa, S F, primary, Tyndall, D A, additional, and Ouellette, T E, additional
- Published
- 1990
- Full Text
- View/download PDF
6. Assessing energy requirements of patients on respirators.
- Author
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Mccamish, Mark A., Dean, Richard E., Ouellette, Thomas R., McCamish, M A, Dean, R E, and Ouellette, T R
- Published
- 1981
- Full Text
- View/download PDF
7. Vibrational spectra of molybdenum and tungsten pentafluorides.
- Author
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Ouellette, T. J., Ratcliffe, C. T., and Sharp, D. W. A.
- Published
- 1969
- Full Text
- View/download PDF
8. Preparation and complexes of molybdenum pentafluoride and molybdenum(V) bromide tetrafluoride.
- Author
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Mercer, M., Ouellette, T. J., Ratcliffe, C. T., and Sharp, D. W. A.
- Published
- 1969
- Full Text
- View/download PDF
9. Assessment of caloric needs in stressed patients.
- Author
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Paauw, J D, primary, McCamish, M A, additional, Dean, R E, additional, and Ouellette, T R, additional
- Published
- 1984
- Full Text
- View/download PDF
10. ChemInform Abstract: DIAETHYLAMIDOWOLFRAM(VI)‐FLUORIDE
- Author
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MAJID, A., primary, MCLEAN, R. R., additional, OUELLETTE, T. J., additional, SHARP, D. W. A., additional, and WINFIELD, J. M., additional
- Published
- 1971
- Full Text
- View/download PDF
11. Detecting the effect of genetic diversity on brain composition in an Alzheimer's disease mouse model.
- Author
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Gurdon B, Yates SC, Csucs G, Groeneboom NE, Hadad N, Telpoukhovskaia M, Ouellette A, Ouellette T, O'Connell KMS, Singh S, Murdy TJ, Merchant E, Bjerke I, Kleven H, Schlegel U, Leergaard TB, Puchades MA, Bjaalie JG, and Kaczorowski CC
- Subjects
- Animals, Mice, Mice, Transgenic, Amyloid beta-Peptides metabolism, Amyloid beta-Peptides genetics, Male, Alzheimer Disease genetics, Alzheimer Disease pathology, Alzheimer Disease metabolism, Disease Models, Animal, Brain metabolism, Brain pathology, Genetic Variation
- Abstract
Alzheimer's disease (AD) is broadly characterized by neurodegeneration, pathology accumulation, and cognitive decline. There is considerable variation in the progression of clinical symptoms and pathology in humans, highlighting the importance of genetic diversity in the study of AD. To address this, we analyze cell composition and amyloid-beta deposition of 6- and 14-month-old AD-BXD mouse brains. We utilize the analytical QUINT workflow- a suite of software designed to support atlas-based quantification, which we expand to deliver a highly effective method for registering and quantifying cell and pathology changes in diverse disease models. In applying the expanded QUINT workflow, we quantify near-global age-related increases in microglia, astrocytes, and amyloid-beta, and we identify strain-specific regional variation in neuron load. To understand how individual differences in cell composition affect the interpretation of bulk gene expression in AD, we combine hippocampal immunohistochemistry analyses with bulk RNA-sequencing data. This approach allows us to categorize genes whose expression changes in response to AD in a cell and/or pathology load-dependent manner. Ultimately, our study demonstrates the use of the QUINT workflow to standardize the quantification of immunohistochemistry data in diverse mice, - providing valuable insights into regional variation in cellular load and amyloid deposition in the AD-BXD model., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
12. The Use of MRI to Detect Malignancy in a Patient Presenting With Unilateral Bloody Nipple Discharge.
- Author
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Romero KN, Ouellette T, Patel R, and Patel T
- Abstract
Nipple discharge presents as either physiological, which is green, white, or yellow, or is considered pathological, which is typically unilateral, spontaneous, and bloody. Bloody nipple discharge (BND) can be due to underlying malignancy or premalignant lesions. Mammogram (MMG), ultrasound (US), MRI, and ductography are all used to evaluate BND, but different modalities offer greater value in the diagnostic process. Here, we present a case that demonstrates the ability of MRI to detect abnormalities not seen on MMG and US in the setting of BND due to underlying malignancy. The use of MRI earlier in the diagnostic process allows for the use of breast-conserving measures and decreases the possibility of metastasis. This would result in less of a need for more aggressive treatments., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2023, Romero et al.)
- Published
- 2023
- Full Text
- View/download PDF
13. Prompt Management of Airbag Burn Injuries Leads to Optimized Patient Outcomes: A Pilot Study.
- Author
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Schalet GN, Tannenbaum SL, Ouellette T, and Fletcher JW
- Abstract
Background: Dual airbags are required to be installed and available for use in all motor vehicles since 1997. The National Highway Traffic Safety Administration reported that 50,457 lives were saved by airbags from 1987 to 2017; however, airbag deployment can cause injuries, including thermal and chemical burns, hyperpigmentation, and dysaesthesia. There is little information available in the literature regarding differences in outcomes between promptly visiting a plastic surgeon and waiting for treatment, especially as an injury may not be immediately apparent or patients may not know that airbag burn injuries may be delayed in presenting., Methods: This is a retrospective cohort pilot study conducted among 14 patients who presented to a plastic surgeon between January 1, 2019 and June 30, 2022 owing to injuries from airbag deployment. An early visit was considered ≤30 days, and a late visit was >30 days. Other variables collected included age, sex, Fitzpatrick skin type, smoking status, comorbidities, type of injury, injury site, pain status, hyper/hypopigmentation, dysaesthesia, epithelialization, and improvements in pain, pigmentation, and dysaesthesia from treatment., Results: The mean age was 36.0 years (standard deviation (SD) 17.9). The majority were female (85.7%), non-smokers (87.5%), and not diabetic (75.0%). Only six patients (42.9%) visited their doctor within one month of injury. Most patients experienced dysaesthesia (85.7%) and pain (71.4%). Thirteen of the 14 patients had hyperpigmentation or hyperemia, and one had hypopigmentation. Full or slight epithelialization was seen in 35.7%, and nine of the 14 patients had no epithelialization. Ongoing issues were a factor for 64.3% of these patients; 42.9% had ongoing issues with hyperpigmentation. A full recovery was seen in 28.6% of the patients. The patients who saw the plastic surgeon by day 30 or less (early) from the time of injury had a 66.7% improvement in pigmentation and 33.3% resolution in pain. Of those who went to the surgeon beyond 30 days (late), 25% had improvement in pigmentation and 37.5% had resolution of pain. Improvement in dysaesthesia occurred in both groups, but those who saw the plastic surgeon early had 33.3% resolution, while 37.5% of those who went late improved. Of those who went late to the surgeon, only 12.5% had epithelialization, while 66.7% of those who went within 30 days showed signs of (full or slight) epithelialization., Conclusion: Patients involved in motor vehicle collisions (MVCs) should be informed of the delayed fashion in which airbag burns can develop. An ostensibly mild burn may portend long-term consequences, especially if such injuries are not addressed in a prompt manner. Our study demonstrates how airbag burn injuries and their sequelae are best addressed with early care., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2023, Schalet et al.)
- Published
- 2023
- Full Text
- View/download PDF
14. Detecting the effect of genetic diversity on brain composition in an Alzheimer's disease mouse model.
- Author
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Gurdon B, Yates SC, Csucs G, Groeneboom NE, Hadad N, Telpoukhovskaia M, Ouellette A, Ouellette T, O'Connell K, Singh S, Murdy T, Merchant E, Bjerke I, Kleven H, Schlegel U, Leergaard TB, Puchades MA, Bjaalie JG, and Kaczorowski CC
- Abstract
Alzheimer's disease (AD) is characterized by neurodegeneration, pathology accumulation, and progressive cognitive decline. There is significant variation in age at onset and severity of symptoms highlighting the importance of genetic diversity in the study of AD. To address this, we analyzed cell and pathology composition of 6- and 14-month-old AD-BXD mouse brains using the semi-automated workflow (QUINT); which we expanded to allow for nonlinear refinement of brain atlas-registration, and quality control assessment of atlas-registration and brain section integrity. Near global age-related increases in microglia, astrocyte, and amyloid-beta accumulation were measured, while regional variation in neuron load existed among strains. Furthermore, hippocampal immunohistochemistry analyses were combined with bulk RNA-sequencing results to demonstrate the relationship between cell composition and gene expression. Overall, the additional functionality of the QUINT workflow delivers a highly effective method for registering and quantifying cell and pathology changes in diverse disease models., Competing Interests: Declaration of Interests The authors declare no competing interests.
- Published
- 2023
- Full Text
- View/download PDF
15. Bilateral Renal Vein Thrombosis in Membranous Nephropathy: Hypoalbuminemia Predictive of Venous Thromboembolism in Nephrotic Syndrome.
- Author
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Casey D, Romero K, Patel R, Ouellette T, Anasseri S, and Eftekhari P
- Abstract
Nephrotic syndrome is a known clinical syndrome in which there is increased permeability in the glomerular basement membrane leading to proteinuria, >3.5g/24h, and hypoalbuminemia. The primary causes of nephrotic syndrome include membranous nephropathy, focal segmental glomerulosclerosis, and minimal change disease. Secondary causes include lupus nephritis, diabetes mellitus, multiple myeloma, amyloidosis, and other systemic conditions. Clinically, nephrotic syndrome presents with edema, hyperlipidemia, and increased risk of thromboembolism, the primary focus of this paper. Nephrotic syndrome is often associated with thromboembolic events, especially in patients with membranous nephropathy. It has been shown that hypoalbuminemia is the most significant independent predictor of venous thromboembolic risk. We present the case of a 32-year-old male who first presented with pleuritic chest pain and was found to have multiple bilateral pulmonary emboli treated with oral anticoagulation. On subsequent visits, prompted by either chest pain or edema, he was found to have increasing pulmonary emboli, as well as downtrending serum albumin levels at each visit. Eventually, bilateral non-occlusive renal vein thrombi were discovered. Lab work indicated membranous nephropathy as the most likely etiology secondary to the patient's presentation. Serum anti-phospholipase A2 receptor antibody positivity confirmed the diagnosis, and the patient was treated appropriately., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Casey et al.)
- Published
- 2022
- Full Text
- View/download PDF
16. Kocuria rosea Bacteremia in a Sickle Cell Patient: A Case Report.
- Author
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Nudelman BG, Ouellette T, Nguyen KQ, Schmaus WH, and Chokshi RR
- Abstract
Central line-associated bloodstream infections (CLABSI) are a significant risk factor for poor patient outcomes. It is important to correctly diagnose and treat these infections to ensure the best chance of recovery. Kocuria rosea is a novel bacteria that is native to the human flora and has been on the rise as a culprit in recent nosocomial infections. However, due to its characteristics, it is often misclassified by commonly used hospital tests. We present a case of a 55-year-old female with sickle cell disease who developed a K. rosea infection during her hospital course and was effectively diagnosed and treated. This case report aims to bring awareness to this unusual bacteria as a possible cause of inpatient infection and CLABSI. Further research should be conducted to determine the incidence of this bacteria and the best testing to be done for its proper recognition., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Nudelman et al.)
- Published
- 2022
- Full Text
- View/download PDF
17. Novel Tool to Monitor Adherence to Oral Oncolytics: A Pilot Study.
- Author
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Sun W, Reeve R, Ouellette T, Stutsky M, De Jesus R, Huffer MJ, and Mougalian SS
- Subjects
- Administration, Oral, Humans, Pilot Projects, Surveys and Questionnaires, Medication Adherence, Text Messaging
- Abstract
Purpose: Nonadherence is a significant issue in cancer care, especially as more oral therapies become available. Measuring and optimizing adherence to such therapies is challenging. In this study, we tested a novel technology that records real-time medication-taking behavior from a smart prescription bottle and can communicate with patients via text message to intervene in cases of nonadherence., Methods: We conducted a 28-patient pilot study to assess the feasibility of this technology in measuring and improving adherence in patients taking capecitabine, an oral chemotherapy agent with a complex, cyclical regimen. The study had a preintervention stage, during which patients were monitored, and an intervention stage, during which the text messaging intervention was enabled., Results: During preintervention, patients had an average self-adherence of 89%, and during post intervention, they had an average adherence of 90%. We defined three categories of patients by change in adherence: category 1 (> 8%), category 2 (-8% to 8%), and category 3 (< -8%). Patients in category 1 tended to live in regions with lower average household income (mean = $58,937 in US dollars [USD]) than those in category 2 (mean = $77,482 USD) and category 3 (mean = $90,972 USD). Of poststudy survey respondents, most indicated that they would want to continue using this technology and that they would recommend it to others., Conclusion: This novel technology is able to monitor, measure, and intervene for patients taking capecitabine in real time. Adherence overall was high, and some patients appeared to benefit more from text-message interventions. Future work should focus on patients deemed high risk for nonadherence., Competing Interests: Rachel De JesusStock and Other Ownership Interests: SMRxT Inc, AbbvieConsulting or Advisory Role: HivePath Michael J. HufferEmployment: SMRxT IncLeadership: SMRxT IncStock and Other Ownership Interests: SMRxT Inc Sarah S. MougalianStock and Other Ownership Interests: Gilead Sciences, Coronado Biosciences, RocheConsulting or Advisory Role: Eisai, Puma Biotechnology, CelgeneResearch Funding: Genentech, PfizerNo other potential conflicts of interest were reported.
- Published
- 2021
- Full Text
- View/download PDF
18. Macrocyclic Peptides that Selectively Inhibit the Mycobacterium tuberculosis Proteasome.
- Author
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Zhang H, Hsu HC, Kahne SC, Hara R, Zhan W, Jiang X, Burns-Huang K, Ouellette T, Imaeda T, Okamoto R, Kawasaki M, Michino M, Wong TT, Toita A, Yukawa T, Moraca F, Vendome J, Saha P, Sato K, Aso K, Ginn J, Meinke PT, Foley M, Nathan CF, Darwin KH, Li H, and Lin G
- Subjects
- Anti-Bacterial Agents chemistry, Anti-Bacterial Agents pharmacology, Drug Design, Humans, Mycobacterium tuberculosis drug effects, Peptides, Cyclic chemistry, Structure-Activity Relationship, Mycobacterium tuberculosis enzymology, Peptides, Cyclic pharmacology, Proteasome Endopeptidase Complex metabolism, Proteasome Inhibitors chemistry, Proteasome Inhibitors pharmacology
- Abstract
Treatment of tuberculosis (TB) currently takes at least 6 months. Latent Mycobacterium tuberculosis (Mtb) is phenotypically tolerant to most anti-TB drugs. A key hypothesis is that drugs that kill nonreplicating (NR) Mtb may shorten treatment when used in combination with conventional drugs. The Mtb proteasome (Mtb20S) could be such a target because its pharmacological inhibition kills NR Mtb and its genetic deletion renders Mtb unable to persist in mice. Here, we report a series of macrocyclic peptides that potently and selectively target the Mtb20S over human proteasomes, including macrocycle 6 . The cocrystal structure of macrocycle 6 with Mtb20S revealed structural bases for the species selectivity. Inhibition of 20S within Mtb by 6 dose dependently led to the accumulation of Pup-tagged GFP that is degradable but resistant to depupylation and death of nonreplicating Mtb under nitrosative stress. These results suggest that compounds of this class have the potential to develop as anti-TB therapeutics.
- Published
- 2021
- Full Text
- View/download PDF
19. A pharmacological characterization of Cannabis sativa chemovar extracts.
- Author
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Devsi A, Kiyota B, Ouellette T, Hegle AP, Rivera-Acevedo RE, Wong J, Dong Y, Pugsley MK, and Fung T
- Abstract
Background: Cannabis contains Δ
9 -tetrahydrocannabinol (Δ9 -THC) and cannabidiol (CBD) as the primary constituents responsible for pharmacological activity. However, there are numerous additional chemically-related structures to Δ9 -THC and CBD that are pharmacologically active and may influence the pharmacological properties of Δ9 -THC and CBD. This study chemically characterized the cannabinoid constituents in a series of cannabis chemovar extracts and investigated the potential cannabinoid entourage effect in two behavioral assays., Methods: Six chemovar extracts were compared to pure Δ9 -THC, CBD and morphine for effects on the following behavioral assays in mice: hot plate and tail suspension. The battery of behavioral tests was conducted post intravenous administration of cannabis chemovar extract. Cannabinoid profiles of extracts were analyzed using high performance liquid chromatography. Cannabis extracts were administered at equal doses of Δ9 -THC to investigate the role of their cannabinoid profiles in modulating the effects of Δ9 -THC. Dose response curves were fit using a log[inhibitor] vs response three parameter model and differences between group means were determined using a one-way ANOVA followed by a post hoc test., Results: Cannabis chemovars tested in this study exhibited substantially different cannabinoid profiles. All chemovars produced dose-dependent immobility in the tail suspension assay and dose-dependent antinociception in the hot plate assay. The maximum antinociceptive effect and ED50 was comparable between cannabis chemovars and Δ9 -THC. Two cannabis chemovars produced significantly greater immobility in the tail suspension test, with no significant differences in ED50., Conclusions: Commercially available cannabis chemovars vary widely in cannabinoid content, but when equalized for Δ9 -THC content, they produce similar behavioral effects with two exceptions. These findings provide only limited support for the entourage hypothesis. Further studies are necessary to characterize the nature of these pharmacological differences between cannabis chemovars and pure Δ9 -THC.- Published
- 2020
- Full Text
- View/download PDF
20. Context-Dependent Sensory Processing across Primary and Secondary Somatosensory Cortex.
- Author
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Condylis C, Lowet E, Ni J, Bistrong K, Ouellette T, Josephs N, and Chen JL
- Subjects
- Animals, Male, Mice, Models, Neurological, Neurons physiology, Somatosensory Cortex cytology, Vibrissae cytology, Vibrissae physiology, Memory, Short-Term, Somatosensory Cortex physiology, Touch Perception
- Abstract
To interpret the environment, our brain must evaluate external stimuli against internal representations from past experiences. How primary (S1) and secondary (S2) somatosensory cortices process stimuli depending on recent experiences is unclear. Using simultaneous multi-area population imaging of projection neurons and focal optogenetic inactivation, we studied mice performing a whisker-based working memory task. We find that activity reflecting a current stimulus, the recollection of a previous stimulus (cued recall), and the stimulus category are distributed across S1 and S2. Despite this overlapping representation, S2 is important for processing cued recall responses and transmitting these responses to S1. S2 network properties differ from S1, wherein S2 persistently encodes cued recall and the stimulus category under passive conditions. Although both areas encode the stimulus category, only information in S1 is important for task performance through pathways that do not necessarily include S2. These findings reveal both distributed and segregated roles for S1 and S2 in context-dependent sensory processing., Competing Interests: Declaration of Interests The authors declare no competing interests., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
21. Selective Phenylimidazole-Based Inhibitors of the Mycobacterium tuberculosis Proteasome.
- Author
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Zhan W, Hsu HC, Morgan T, Ouellette T, Burns-Huang K, Hara R, Wright AG, Imaeda T, Okamoto R, Sato K, Michino M, Ramjee M, Aso K, Meinke PT, Foley M, Nathan CF, Li H, and Lin G
- Subjects
- Imidazoles chemistry, Microbial Sensitivity Tests, Mycobacterium tuberculosis enzymology, Proteasome Inhibitors chemistry, Reactive Nitrogen Species metabolism, Structure-Activity Relationship, Imidazoles pharmacology, Mycobacterium tuberculosis drug effects, Proteasome Inhibitors pharmacology
- Abstract
Proteasomes of pathogenic microbes have become attractive targets for anti-infectives. Coevolving with its human host, Mycobacterium tuberculosis (Mtb) has developed mechanisms to resist host-imposed nitrosative and oxidative stresses. Genetic deletion or pharmacological inhibition of the Mtb proteasome (Mtb20S) renders nonreplicating Mtb susceptible to reactive nitrogen species in vitro and unable to survive in the lungs of mice, validating the Mtb proteasome as a promising target for anti-Mtb agents. Using a structure-guided and flow chemistry-enabled study of structure-activity relationships, we developed phenylimidazole-based peptidomimetics that are highly potent for Mtb20S. X-ray structures of selected compounds with Mtb20S shed light on their selectivity for mycobacterial over human proteasomes.
- Published
- 2019
- Full Text
- View/download PDF
22. Improvement of Asparagine Ethylenediamines as Anti-malarial Plasmodium -Selective Proteasome Inhibitors.
- Author
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Zhan W, Visone J, Ouellette T, Harris JC, Wang R, Zhang H, Singh PK, Ginn J, Sukenick G, Wong TT, Okoro JI, Scales RM, Tumwebaze PK, Rosenthal PJ, Kafsack BFC, Cooper RA, Meinke PT, Kirkman LA, and Lin G
- Subjects
- Antimalarials chemistry, Antimalarials metabolism, Asparagine chemistry, Asparagine metabolism, Drug Resistance drug effects, Drug Resistance genetics, Ethylenediamines chemistry, Ethylenediamines metabolism, Humans, Malaria, Falciparum metabolism, Malaria, Falciparum parasitology, Mutation, Plasmodium falciparum genetics, Plasmodium falciparum physiology, Proteasome Endopeptidase Complex genetics, Antimalarials pharmacology, Malaria, Falciparum prevention & control, Plasmodium falciparum drug effects, Proteasome Endopeptidase Complex metabolism, Proteasome Inhibitors pharmacology
- Abstract
The Plasmodium proteasome (Pf20S) emerged as a target for antimalarials. Pf20S inhibitors are active at multiple stages of the parasite life cycle and synergize with artemisinins, suggesting that Pf20S inhibitors have potential to be prophylactic, therapeutic, and transmission blocking as well as are useful for combination therapy. We recently reported asparagine ethylenediamines (AsnEDAs) as immunoproteasome inhibitors and modified AsnEDAs as selective Pf20S inhibitors. Here, we report further a structure-activity relationship study of AsnEDAs for selective inhibition of Pf20S over human proteasomes. Additionally, we show new mutation that conferred resistance to AsnEDAs and collateral sensitivity to an inhibitor of the Pf20S β2 subunit, the same as previously identified resistant mutation. This resistance could be overcome through the use of the structure-guided inhibitor design. Collateral sensitivity to inhibitors among respective proteasome subunits underscores the potential value of treating malaria with combinations of inhibitors of different proteasome subunits to minimize the emergence of drug resistance.
- Published
- 2019
- Full Text
- View/download PDF
23. Isolated avulsion fracture of the extensor carpi radialis brevis insertion due to a boxer's injury.
- Author
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Breeze SW, Ouellette T, and Mays MM
- Subjects
- Athletic Injuries, Boxing, Humans, Magnetic Resonance Imaging, Male, Metacarpus diagnostic imaging, Metacarpus surgery, Radiography, Tendon Injuries diagnostic imaging, Wrist Injuries diagnostic imaging, Young Adult, Metacarpus injuries, Tendon Injuries surgery, Wrist Injuries surgery
- Abstract
Avulsion fractures involving the radial wrist extensor tendons are extremely rare. Only 8 avulsion fractures of the extensor carpi radialis longus and 7 avulsion fractures of the extensor carpi radialis brevis have been previously reported. We know of no case involving an isolated avulsion fracture of the base of the third metacarpal where the extensor carpi radialis brevis attachment to the fracture fragment was still completely intact. This article reports a patient with an isolated avulsion fracture of the extensor carpi radialis brevis insertion treated by open reduction and internal fixation. The avulsion fracture was the result of a poor punching technique. The patient healed uneventfully, and returned to full painless activity. The senior author (S.W.B.) of this article is a former Tae Kwon Doe instructor and includes some editorial comments on proper technique for punching to avoid injury.
- Published
- 2009
24. Production and purification of refolded recombinant human IL-7 from inclusion bodies.
- Author
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Ouellette T, Destrau S, Ouellette T, Zhu J, Roach JM, Coffman JD, Hecht T, Lynch JE, and Giardina SL
- Subjects
- Electrophoresis, Polyacrylamide Gel, Escherichia coli, Fermentation, Humans, Interleukin-7 genetics, Interleukin-7 pharmacology, Protein Denaturation, Protein Renaturation, Quality Control, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins isolation & purification, Recombinant Proteins pharmacology, Inclusion Bodies chemistry, Interleukin-7 chemistry, Interleukin-7 isolation & purification, Protein Folding
- Abstract
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by homogenization with 6 M guanidine HCl. Attempts at refolding rhIL-7 from solubilized inclusion bodies without prior purification of monomeric, denatured rhIL-7 were not successful. Denatured, monomeric rhIL-7 was therefore initially purified by size-exclusion chromatography using Prep-Grade Pharmacia Superdex 200. Correctly folded rhIL-7 monomer was generated by statically refolding the denatured protein at a final protein concentration of 80-100 microg/ml in 100 mM Tris, 2mM EDTA, 500 mM L-arginine, pH 9.0, buffer with 0.55 g/l oxidized glutathione at 2-8 degrees C for at least 48 h. The refolded rhIL-7 was subsequently purified by low-pressure liquid chromatography, using a combination of hydrophobic interaction, cation-exchange, and size-exclusion chromatography. The purified final product was >95% pure by SDS-PAGE stained with Coomassie brilliant blue, high-pressure size-exclusion chromatography (SEC-HPLC), and reverse-phase HPLC. The endotoxin level was <0.05 EU/mg. The final purified product was biologically active in a validated IL-7 dependent pre-B-cell bioassay. In anticipation of human clinical trials, this material is currently being evaluated for safety and efficacy in non-human primate toxicology studies.
- Published
- 2003
- Full Text
- View/download PDF
25. Bibliography database managers. A comparative review.
- Author
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Nicoll LH, Ouellette TH, Bird DC, Harper J, and Kelley J
- Subjects
- Humans, Bibliographies as Topic, Database Management Systems organization & administration, Software Validation
- Abstract
Bibliography database managers (BDMs) are used to manage information resources: specifically, to maintain a database of references and create bibliographies and reference lists for written works. This comparative review provides an overview of BDMs and capsule reviews of five programs: EndNote Plus 2.0; Library Master 3.0; Papyrus 7.0.11; Pro-Cite 2.2.1; and Reference Manager 6.02.
- Published
- 1996
26. Application of added beam filtration to intra-oral radiography.
- Author
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Kapa SF, Tyndall DA, and Ouellette TE
- Subjects
- Erbium, Filtration, Gadolinium, Humans, Radiation Dosage, X-Ray Film, Yttrium, Radiography, Dental methods, X-Ray Intensifying Screens
- Published
- 1990
- Full Text
- View/download PDF
27. A method to forecast labor allocation requirements in a respiratory therapy service.
- Author
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Ouellette TR
- Subjects
- Time and Motion Studies, Hospital Departments organization & administration, Personnel Management methods, Personnel Staffing and Scheduling methods, Respiratory Therapy Department, Hospital organization & administration
- Published
- 1975
28. Topical anesthesia to improve patient tolerance of artificial airways during mechanical ventilation.
- Author
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Wilbur HO and Ouellette TR
- Subjects
- Female, Humans, Intubation, Intratracheal methods, Lidocaine, Male, Middle Aged, Tracheotomy methods, Anesthesia, Local statistics & numerical data, Respiration, Artificial methods
- Published
- 1976
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