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2. A widespread group of large plasmids in methanotrophic Methanoperedens archaea

Author

Schoelmerich, Marie C, Ouboter, Heleen T, Sachdeva, Rohan, Penev, Petar I, Amano, Yuki, West-Roberts, Jacob, Welte, Cornelia U, and Banfield, Jillian F

Subjects
Biotechnology, Genetics, Archaea, Anaerobiosis, Methane, Oxidation-Reduction, Plasmids
Abstract

Anaerobic methanotrophic (ANME) archaea obtain energy from the breakdown of methane, yet their extrachromosomal genetic elements are little understood. Here we describe large plasmids associated with ANME archaea of the Methanoperedens genus in enrichment cultures and other natural anoxic environments. By manual curation we show that two of the plasmids are large (155,605 bp and 191,912 bp), circular, and may replicate bidirectionally. The plasmids occur in the same copy number as the main chromosome, and plasmid genes are actively transcribed. One of the plasmids encodes three tRNAs, ribosomal protein uL16 and elongation factor eEF2; these genes appear to be missing in the host Methanoperedens genome, suggesting an obligate interdependence between plasmid and host. Our work opens the way for the development of genetic vectors to shed light on the physiology and biochemistry of Methanoperedens, and potentially genetically edit them to enhance growth and accelerate methane oxidation rates.

Published
2022

5. Unraveling Nitrogen, Sulfur, and Carbon Metabolic Pathways and Microbial Community Transcriptional Responses to Substrate Deprivation and Toxicity Stresses in a Bioreactor Mimicking Anoxic Brackish Coastal Sediment Conditions

Author

Dalcin Martins, Paula, Echeveste Medrano, M.J., Arshad, A., Kurth, Julia M., Ouboter, Heleen T., Camp, Huub J.M. op den, Jetten, Mike S.M., Welte, Cornelia U., Dalcin Martins, Paula, Echeveste Medrano, M.J., Arshad, A., Kurth, Julia M., Ouboter, Heleen T., Camp, Huub J.M. op den, Jetten, Mike S.M., and Welte, Cornelia U.

Abstract

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Published
2022

6. Methane-Dependent Extracellular Electron Transfer at the Bioanode by the Anaerobic Archaeal Methanotroph “Candidatus Methanoperedens”

Author

Ouboter, Heleen T., Berben, Tom, Berger, Stefanie, Jetten, Mike S.M., Sleutels, Tom, ter Heijne, Annemiek, Welte, Cornelia U., Ouboter, Heleen T., Berben, Tom, Berger, Stefanie, Jetten, Mike S.M., Sleutels, Tom, ter Heijne, Annemiek, and Welte, Cornelia U.

Abstract

Anaerobic methanotrophic (ANME) archaea have recently been reported to be capable of using insoluble extracellular electron acceptors via extracellular electron transfer (EET). In this study, we investigated EET by a microbial community dominated by “Candidatus Methanoperedens” archaea at the anode of a bioelectrochemical system (BES) poised at 0 V vs. standard hydrogen electrode (SHE), in this way measuring current as a direct proxy of EET by this community. After inoculation of the BES, the maximum current density was 274 mA m–2 (stable current up to 39 mA m–2). Concomitant conversion of 13CH4 into 13CO2 demonstrated that current production was methane-dependent, with 38% of the current attributed directly to methane supply. Based on the current production and methane uptake in a closed system, the Coulombic efficiency was about 17%. Polarization curves demonstrated that the current was limited by microbial activity at potentials above 0 V. The metatranscriptome of the inoculum was mined for the expression of c-type cytochromes potentially used for EET, which led to the identification of several multiheme c-type cytochrome-encoding genes among the most abundant transcripts in “Ca. Methanoperedens.” Our study provides strong indications of EET in ANME archaea and describes a system in which ANME-mediated EET can be investigated under laboratory conditions, which provides new research opportunities for mechanistic studies and possibly the generation of axenic ANME cultures.

Published
2022

8. Unraveling Nitrogen, Sulfur, and Carbon Metabolic Pathways and Microbial Community Transcriptional Responses to Substrate Deprivation and Toxicity Stresses in a Bioreactor Mimicking Anoxic Brackish Coastal Sediment Conditions

Author

Dalcin Martins, Paula, primary, Echeveste Medrano, Maider J., additional, Arshad, Arslan, additional, Kurth, Julia M., additional, Ouboter, Heleen T., additional, Op den Camp, Huub J. M., additional, Jetten, Mike S. M., additional, and Welte, Cornelia U., additional

Published
2022
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