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Salvia microphylla is a known species due to its broad uses in traditional medicine against memory loss and rheumatism. The knowledge regarding the chemical composition and biological activities of the species collected in Algeria, no studies have been reported in the literature. Therefore, the present work focuses on the characterization of the chemical composition of the essential oils (EOs) and the determination of the antioxidant, anticholinesterase, α-glucosidase, and antimicrobial activities of Salvia microphylla. The EOs were obtained by hydrodistillation from the aerial parts, leaves and stems and submitted to chemical analysis by GC and GC/MS. The β-Caryophyllene was identified as the main constituent in the aerial parts and leaves essential oils with 16.75 ± 0.02 % and 17.86 ± 0.07 %, respectively. Likewise, the α-Eudesmol was the predominant component in the stems oil with (21.47 ± 0.20 %). The antioxidant activity of EOs was estimated through using four comparative methods: DPPH, ABTS•+, Reducing power and CUPRAC assays. The Stems oil was the most active one in CUPRAC assay, with an IC50 value with 7.72 ± 0.43 µg/mL. The enzyme inhibitory activity of the essential oils was realized against key enzymes involved in type 2 diabetes (α-glucosidase) using 4-Nitrophenyl-α-d-glucopyranoside as substrate and in neurodegenerative (AChE and BChE) diseases. The highest anticholinesterase activity against acetylcholinesterase was observed in the EO of aerial parts essential (IC50: 23.65 ± 0.73 µg/mL). The EO isolated from stems (IC50: 37.07 ± 1.44 µg/mL) exhibited a butyrylcholinesterase activity very close to that of analytical standard galantamine (IC50: 34.75 ± 1.99 µg/mL). Furthermore, all EOs displayed high inhibitory activity against α-glucosidase, better to that of the standard acarbose. The EOs of Salvia microphylla display potential properties against type 2 diabetes. A broth microdilution method was used to evaluate the antimicrobial activity of Salvia microphylla EOs, against eleven microbial strains and two yeast. The EOs showed better antibacterial activity against Gram-positive and Gram-negative bacteria except for Pseudomonas aeruginosa, with the stems essential oil being more efficient. Moreover, significant antifungal activity was observed against Candida albicans. Resumen. Salvia microphylla es una specie conocida debido a su amplio uso en medicina tradicional, contra la pérdida de memoria y el reumatismo. En el caso de la especie de planta recolectada en Algeria, no hay datos publicados sobre su composición química y sus actividades biológicas. Por ello, el presente trabajo ha sido enfocado en la caracterización de la composición química de aceites esenciales (EOs) de Salvia microphylla y en la determinación de sus actividades antioxidante, anticolinesterasa, α-glucosidasa y antimicrobiano. Los EOs fueron obtenidos mediante hidrodestilación de las partes aéreas, ojas y tallos, y fueron sometidos al análisis por cromatografía de gases con detección por ionización en flama y por espectrometría de masas. Se identificó a β-cariofileno como el componente principal de los aceites de las partes aéreas y de tallos con concentraciones de 16.75 ± 0.02 % y 17.86 ± 0.07 %, respectivamente. Por su parte, el α-Eudesmol fue encontrado como componente predominante en aceite de tallos (21.47 ± 0.20 %). La actividad antioxidante de los EOs fue estimada en base a cuatro métodos compartivos: DPPH, ABTS•+, poder reductor y ensayo CUPRAC. El aceite de tallos resultó ser el más activo en ensayo CUPRAC, con el valor IC50 de 7.72 ± 0.43 µg/mL. La actividad inhibitora de enzimas de los EOs fue evaluada contra principales enzimas involucrados en diabetes tipo 2 (α-glucosidasa), utilizando 4-Nitrofenil-α-d-glucopiranosida como sustrato, y en enfermedades neurodergenerativas (AChE y BChE). La mayor actividad anticolinesterasa y acetilcolinesterasa fue observada en el EO de partes aéreas (IC50: 23.65 ± 0.73 µg/mL). El EO islado de tallos (IC50: 37.07 ± 1.44 µg/mL) presentó actividad de butirilcolinestarasa muy similar a la del estándar analítico, galantamina (IC50: 34.75 ± 1.99 µg/mL). Aunado a ello, todos EOs presentaron una alta actividad inhibitora contra α-glucosidasa, que era mejor comparando con la del estándar de acarbosa. Los EOs de Salvia microphylla presentan potenciales propiedades contra diabetes tipo 2. Para evaluar la actividad antimicrobiana de los EOs de Salvia microphylla, se utilizó el método de microdulución en caldo, contra once sepas microbianas y dos de levadura. La mejor actividad se observó contra bacterias Gram-positivas y Gram-negativas, excepto Pseudomonas aeruginosa, los cuales presentaron alta resistencia. Los EOs presentaron también importante actividad antifungica contra Candida albicans.

The authors thank the DG/RSDT, MESRS, Algeria for financial support.

Teurium atratum (Lamiaceae) is an endemic species growing in northeastern Algeria, where it is used in a mint tea drink. The volatile compounds obtained by hydrodistillation of aerial parts of T. atratum were analyzed by GC and GC/MS. Thirty compounds were characterized, representing 96.9% of the essential oil, with T-cadinol (45.8%), thymol (22.7%) and carvacrol (14.0%) as the main components.

International audience; Thirteen compounds (flavonoids, phenolic acids and sterols) were isolated from the aerial parts of Hypericum tomentosum L. Their structures were established on the basis of physical and spectroscopic analysis, and by comparison with the literature data. In vivo and in vitro protective effect of the butanolic extract of H. tomentosum (BEHT) against acute oxidative stress induced by a single dose of 20 mg/kg epirubicin (an anticancer drug) was evaluated in female Wistar rat using kidney and heart cytosolic fraction. Scavenging effect of BEHT, lipid peroxidation (MDA), glutathione (GSH) concentration and Catalase activity were measured. BEHT extract restored the renal and heart functions and clearly shows a protective effect against epirubicin-induced oxidative stress.

The hydrodistilled essential oil of Hypericum perfoliatum (Clusiaceae), grown at Jijel (north-eastern Algeria), was analysed by GC–MS; 35 components, representing 96% of the oil, have been identified, with thymol (22.10%), T-cadinol (18.5%) and 4,5-dimethyl-2-ethylphenol (13%) as the main constituents. Copyright © 2005 John Wiley & Sons, Ltd.

The volatile compounds obtained by hydrodistillation of the aerial parts of Rosmarinus officinalis cultivated at the Algerian Sahara were analyzed by GC/MS. Thirty compounds were characterized representing 98.2% of the essential oil with 1,8-cineole (29.5%), 2-ethyl-4,5-dimethylphenol (12.0%) and camphor (11.5%) as the major components.

International audience; Three long-chain unsaturated esters (1-3), a fatty acid (4), a fatty ester (5), phytone (6) and a phloroglucinol (hyperfoliatin) (7) were isolated from the light petroleum extract of the endemic species Hypericum tomentosum L. (Clusiaceae). Compound 2, 8,10,13-trimethyltetradecanoic acid (2E)-3-methylhexadec-2-enyl ester, which we named tomentosate, is reported for the first time. The structures of the identified compounds were established on the basis of physical and spectroscopic analysis, and by comparison with literature data.