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33 results on '"Ou, Changbo"'

22. Identification and assessment of pathogenicity of a naturally reassorted infectious bursal disease virus from Henan, China

Author

Wang, Qiuxia, primary, Hu, Huilong, additional, Chen, Guangli, additional, Liu, Hailin, additional, Wang, Siyuan, additional, Xia, Dasong, additional, Yu, Yan, additional, Zhang, Yanhong, additional, Jiang, Jinqing, additional, Ma, Jinyou, additional, Xu, Yanzhao, additional, Xu, Zhiyong, additional, Ou, Changbo, additional, and Liu, Xingyou, additional

Published
2019
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23. Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus

Author

Pan Qing, Liu Aijing, Zhang Faming, Ling Yong, Ou Changbo, Hou Na, and He Cheng

Subjects
Isolation, Characterisation, Ornithobacterium rhinotracheale, H9N2, Co-infect, Broiler, Veterinary medicine, SF600-1100
Abstract

Abstract Background Since 2008, a progressive pneumonia has become prevalent in broilers and laying hens. This disease occurrs the first day after hatching and lasts more than 30 days, resulting in approximately 70% morbidity and 30% mortality in broilers. The objective of this study was to isolate and identify the pathogens that are responsible for the progressive pneumonia and establish an animal model for drug screening. Results 193 serum samples were collected from 8 intensive farms from 5 provinces in China and analysed in the current research. Our clinical survey showed that 65.2% to 100% of breeding broilers, breeding layers, broilers and laying hens were seropositive for ORT antibodies. From 8 intensive farms, six ORT isolates were identified by PCR and biochemical assays, and two H9N2 viruses were isolated. Newcastle Disease Virus (NDV) and Infectious BronchitisVirus (IBV) were excluded. Typical pneumonia and airsacculitis were observed both in broilers inoculated intraperitoneally with an ORT isolate alone and in those co-infected with ORT and H9N2 virus isolates. Specifically, the survival rate was 30%, 20%, 70%, 50% and 90% in birds inoculated with ORT+H9N2 virus, ORT followed by H9N2 virus, H9N2 virus followed by ORT, and ORT or H9N2 virus alone, respectively. Conclusions The results of this study suggest that ORT infections of domestic poultry have been occurring frequently in China. ORT infection can induce higher economic losses and mortality if H9N2 AIV is also present. Although the isolation of ORT and H9N2 virus has been reported previously, there have been no reported co-infections of poultry with these two pathogens. This is the first report of co-infection of broilers with ORT and H9N2 virus, and this co-infection is probably associated with the outbreak of broiler airsacculitis in China, which has caused extensive economic losses.

Published
2012
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27. Isolation of a novel serotype strain of infectious bronchitis virus ZZ2004 from ducks in China

Author

Liu Jinjing, Sixin Yao, Xingyou Liu, Ou Changbo, Xianwen Wang, and Zonghui Yao

Subjects
0301 basic medicine, Serotype, medicine.medical_specialty, China, animal structures, Infectious bronchitis virus, Spike, Biology, medicine.disease_cause, Serogroup, Article, 03 medical and health sciences, Medical microbiology, Antigen, Virology, Genetics, medicine, Animals, Amino Acid Sequence, Molecular Biology, Gene, Pathogen, Respiratory Tract Infections, Phylogeny, Poultry Diseases, Coronavirus, Phylogenetic analysis, Respiratory tract infections, General Medicine, 030104 developmental biology, Ducks, embryonic structures, RNA, Viral, Coronavirus Infections, Sequence Alignment, M gene
Abstract

In chickens, the infectious bronchitis virus (IBV) often causes respiratory distress, a decrease in egg production, poor egg quality, and occasional nephritis. However, ZZ2004, a Chinese isolate of IBV, was obtained from ducks with clinical growth suppression and mild respiratory symptoms that had been reared with chickens in the central region of China. Virus isolation, virus neutralization testing, and RT-PCR were employed to identify the causative pathogen, while sequence alignment was used to analyze gene variations of the S1 subunit and M genes. The results showed that the ducks were infected with IBV due to the emergence of a dwarfing phenotype and the death of embryos between 48 and 144 h post-inoculation. RT-PCR also confirmed the presence of the expected fragment sizes of the S1 subunit and M genes by RT-PCR. Meanwhile, the results of the virus neutralization test indicated that the strains of JX/99/01, GD, SAIBK, LDT3 showed cross-reactivity with the ZZ2004 isolate, and hardly any cross-neutralization of IBV ZZ2004 was observed with the strains of M41, H120, Gray, Holte, or Aust-T. Phylogenetic analysis suggested that there were large differences between ZZ2004 and other IBV reference strains on the S1 subunit. Meanwhile, homologies in the nucleotide and amino acid sequences of the M gene of IBV ZZ2004 were 86.9-92.0 % and 91.1-93.9 %, respectively, compared with 35 other IBV reference strains derived from different regions. This result revealed that there were conspicuous variations among the selected strains. Furthermore, the results showed that the prevalent strains of IBV in ducks had no antigen homology with the vaccine strains widely used in China except the LDT3-strain, making it urgent to explore and develop new IBV vaccines.

Published
2015

28. Dryocrassin ABBA, a novel active substance for use against amantadine-resistant H5N1 avian influenza virus

Author

Ou, Changbo, Zhang, Qiang, Wang, Juan, Wu, Guojiang, Shi, Ningning, He, Cheng, and Gao, Zengping

Subjects
Microbiology (medical), lcsh:QR1-502, Inflammation, medicine.disease_cause, Microbiology, lcsh:Microbiology, Virus, Influenza virus H5N1, Amantadine, medicine, Antiviral activity, Survival rate, Original Research, Dryocrassin ABBA, medicine.diagnostic_test, Inoculation, business.industry, Correction, Virology, phloroglucinol compound, Influenza A virus subtype H5N1, Interleukin 10, Bronchoalveolar lavage, medicine.symptom, business, Rhizoma Dryopteridis Crassirhizomatis, medicine.drug
Abstract

The occurrence of multi-drug resistant highly pathogenic avian influenza virus (HPAIV) strains highlights the urgent need for strategies for the prevention and control of avian influenza virus. The aim of our current study is to evaluate the antiviral activity of dryocrassin ABBA isolated from Rhizoma Dryopteridis Crassirhizomatis (RDC) against an amantadine-resistant H5N1 (A/Chicken/Hebei/706/2005) strain in a mouse model. Post inoculation with HPAIV H5N1 virus in mice, the survival rate was 87%, 80% and 60% respectively in the 33mg/kg, 18mg/kg and 12.5 mg/kg Dryocrassin ABBA-treated groups. On the other hand, the survival rate was 53% and 20%, respectively in the amantadine-treated group and untreated group. Mice administered dryocrassin ABBA or amantadine showed a significant weight increase compared to the untreated group. Moreover, 33 mg/kg and 18 mg/kg dryocrassin ABBA have decreased lung index (P>0.05) and virus loads (P

Published
2015

30. Alleviation of infectious-bursal-disease-virus-induced bursal injury by betaine is associated with DNA methylation in IL-6and interferon regulatory factor 7 promoter

Author

Zhang, Yanhong, Yu, Yan, Ou, Changbo, Ma, Jinyou, Wang, Qiuxia, Du, Shouyang, Xu, Zhiyong, Li, Renfeng, and Guo, Feng

Abstract

Infectious bursal disease virus (IBDV) often infects young chickens and causes severe immunosuppression and inflammatory injury. Betaine is an antiviral and anti-inflammatory ingredient that may exert functions through epigenetic regulation. However, the effects of betaine on an IBDV-induced bursal injury and their underlying mechanisms have not been investigated. In this study, betaine was supplemented to the drinking water of newly hatched commercial broilers for 3 wk. Afterward, the chickens were infected with the IBDV. After 5 D of infection, the bursal lesions were examined. The mRNA expression levels of IBDV VP2 gene, pro-inflammatory cytokines, and interferons were detected. Furthermore, the 5-methylcytosine level of the CpG island in the promoter region of IL-6and interferon regulatory factor 7 (IRF7) were determined. The IBDV induced the depletion of lymphocytes and inflammation in the bursal follicles. IBDV infection considerably elevated the mRNA levels of VP2, IL-6, types I (IFNαand IFNβ) and II (IFNγ) interferons, and IRF7. The CpG island methylation in the promoter regions of IL-6and IRF7were substantially decreased after IBDV infection. Betaine administration attenuated the IBDV-induced bursal lesions. Meanwhile, the IBDV-induced mRNA expression levels of IL-6, IFNβ, and IRF7were suppressed by betaine consumption. Furthermore, the hypomethylation effects of IBDV infection to the promoter regions of IL-6and IRF7genes were eliminated and relieved by betaine administration. Our results indicated that the IBDV-induced expression levels of IL-6and IRF7genes are associated with the suppression of methylation in the promoter region. Betaine administration through drinking water may alleviate the IBDV-induced bursal injury via epigenetic regulation.

Published
2019
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