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199 results on '"Ottomano, Cosimo"'

2. A preliminary proposal for quality control assessment and harmonization of leukocytes morphology-structural parameters (cell population data parameters)

Author

Seghezzi Michela, Buoro Sabrina, Previtali Giulia, Moioli Valentina, Manenti Barbara, Simon-Lopez Ramon, Ottomano Cosimo, and Lippi Giuseppe

Subjects
cell population data, quality control, withinrun imprecision, reference range, stability, Biochemistry, QD415-436
Abstract

Background: The cell population data (CPD) measured by Sysmex XN-9000 can be used for screening many hematological and non-hematological disorders. Since little information is available on harmonization of CPD among different instrumentation and clinical laboratories, this study aimed at assessing the current degree of CPD harmonization between separate Sysmex XN modules allocated to the same laboratory. Methods: A total number of 78291 data were used for verification of within-run imprecision, analyzers harmonization, reference ranges and assessment of blood sample stability of CPD parameters, including results of daily quality control testing and those generated in samples collected from blood donors and healthy volunteers. Results: Within-run imprecision of CPD parameters ranged between 0.4 and 14.1%. Good agreement was found among five different XN-modules, especially when values were adjusted after calculation of instrument-specific alignment factors. The bias of all parameters remained always lower than the reference change values in samples stored for up to 8 hours, regardless of storage temperature. Conclusions: The imprecision of CPD parameters was acceptable, except for those reflecting the dispersion of cellular clusters. Due to the lack of reference control materials, we showed that the use of data generated on a large number of normal routine samples (i.e., a Moving Average population) may be a reliable approach for testing analyzers harmonization. Nevertheless, availability of both calibration and quality control materials for these parameters is highly advisable in the future. We finally showed that whole blood samples may be stable for up to 2 -4 hours for most CPD parameters.

Published
2018

3. Evaluation of residual humoral immune response against SARS-CoV-2 by a surrogate virus neutralization test (sVNT) 9 months after BNT162b2 primary vaccination

Author

Pezzati, Laura, primary, Milazzo, Laura, additional, Carrozzo, Giorgia, additional, Kullmann, Cristina, additional, Oreni, Letizia, additional, Beltrami, Martina, additional, Caronni, Stefania, additional, Lai, Alessia, additional, Caberlotto, Livio, additional, Ottomano, Cosimo, additional, Antinori, Spinello, additional, and Ridolfo, Anna Lisa, additional

Published
2023
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8. Analytical and clinical evaluation of Sysmex UF1000i for automated screening of cerebrospinal fluids

Author

Buoro Sabrina, Ottomano Cosimo, Appassiti Esposito Sara, Gherardi Patrizia, Grazia Alessio Maria, Crippa Alberto, Farina Claudio, Raglio Annibale, and Lippi Giuseppe

Subjects
cerebrospinal fluid, meningitis, automation, cytofluorimeter, Biochemistry, QD415-436
Abstract

Background: We evaluated the performance of Sysmex UF1000i for cell counting and differential cell count, as well as for assessment of bacteria load in cerebrospinal fluid (CSF), as a potential approach for the rapid screening of meningitis or bacterial encephalitis. Methods: We analyzed 77 consecutive CSF samples, 34 of which (44%) displayed leukocyte count >5 white blood cell (WBC)/μL with optical microscopy. Results on the UF-1000i were compared with those obtained by microscopic analysis. Imprecision was evaluated by testing three CSF samples with leukocyte values between 3.5 and 28.8 WBC/μL in 10 replicates. Carry-over was evaluated with the Broughton formula on three CSF pools with leukocyte counts between 93.5 and 132.5 WBC/μL. Linearity was assessed according to CLSI document EP6-A. In the presence of bacteria, identification and antibiogram were performed with Vitex (Biomerieux), except for Neisserie meningitidis (ApiNH, Biomerieux). Sensitivity tests were performed with Vitex and disc diffusion. Results: Optimal correlation was found between UF-1000i and optical microscopy, displaying Pearson's correlation of 0.99 and mean bias of -3.5 WBC/μL (95% CI, from -7.0 to 0.0 WBC/μL). Imprecision varied between 12 and 16%. Linearity was excellent, 4-278 WBC/μL. Carry-over was negligible. ROC analysis yielded AUC of 0.99 for both WBC and bacterial counts. The agreement at threshold >4 WBC/μL was 0.91, with sensitivity and specificity of 1.00 and 0.84. At ≤19 bacteria/μL cut-off, accuracy was 0.98, sensitivity 1.00 and specificity 0.97. Conclusions: According to these results, CSF screening with UF-1000i seems a reliable approach in terms of instrument performance, turnaround time and overall laboratory efficiency.

Published
2014

9. Impact of prior infection status on antibody response to the BNT162b2 mRNA COVID-19 vaccine in healthcare workers at a COVID-19 referral hospital in Milan, Italy

Author

Milazzo, Laura, primary, Pezzati, Laura, additional, Oreni, Letizia, additional, Kullmann, Cristina, additional, Lai, Alessia, additional, Gabrieli, Arianna, additional, Bestetti, Giovanna, additional, Beschi, Cristian, additional, Conti, Federico, additional, Ottomano, Cosimo, additional, Gervasoni, Cristina, additional, Meroni, Luca, additional, Galli, Massimo, additional, Antinori, Spinello, additional, and Ridolfo, Anna Lisa, additional

Published
2021
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12. SARS-CoV-2 antibody responses before and after a third dose of the BNT162b2 vaccine in Italian healthcare workers aged =60 years: One year of surveillance.

Author

Franzese, Monica, Coppola, Luigi, Silva, Romina, Santini, Stefano Angelo, Cinquanta, Luigi, Ottomano, Cosimo, Salvatore, Marco, and Incoronato, Mariarosaria

Subjects
MEDICAL personnel, ANTIBODY formation, COVID-19 vaccines, ANTIBODY titer, SARS-CoV-2
Abstract

This study monitored the anti-spike-receptor-binding domain (RBD) and neutralizing antibodies induced by the Pfizer/BioNTech mRNA BNT162b2 vaccine in a cohort of 163 healthcare workers aged =60 years. We have taken advantage of two study groups, both of whom received the first two doses in the same time window, but Group 1 (54 HCWs) received the third dose 2 months before Group 2 (68 HCWs) did. The cohorts were monitored from the 12th day after the first vaccine dose up to 1 month after the third vaccine dose for a total of eight time points and about 1 year of surveillance (T1 = 12 days after the first dose; T2 = 10 days after the second dose; T3 = 1 month after the second dose; T4 = 3 months after the second dose; T5 = 4 months after the second dose; T6 = 5 months after the second dose; T7 = 7months after the second dose; T8 = 1 month after the third dose for Group 1; T8* = 9 months after the second dose forGroup 2; T9 = 1 month after the third dose for Group 2). The mean value of anti-spike antibodies decreased faster over time, but at T7, its declinewas significantly slowed (T7 vs. T8*). After the third dose, the anti-spike titer rose about 34-fold (T7 vs. T8 and T8* vs. T9) and the booster improved the anti-spike titer by about three times compared with that of the second dose (T3 vs. T8 and T3 vs. T9), and no difference was noted between the two groups. The neutralizing titer was evaluated at T3, T7, T8, and T9. Anti-spike and neutralizing antibodies were found to be strongly correlated (r2 = 0.980; p < 0.001). At T3, 70% of the participants had a neutralizing antibody titer >91% of total anti-spike antibodies that increased to 90% after the third dose (T8 and T9). However, when the anti-spike titer reached its lowest value (T7), the neutralizing antibody levels decreased even further, representing only 44% of total anti-spike antibodies (p < 0.0001). Our findings show that the third vaccine dose improves the humoral response, but the wane of the anti-spike and neutralizing antibody titers over time is more marked in the neutralizing antibodies. [ABSTRACT FROM AUTHOR]

Published
2022
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16. Prevalence of SARS-CoV-2 in an area of unrestricted viral circulation: Mass seroepidemiological screening in Castiglione d’Adda, Italy

Author

Pagani, Gabriele, primary, Giacomelli, Andrea, additional, Conti, Federico, additional, Bernacchia, Dario, additional, Rondanin, Rossana, additional, Prina, Andrea, additional, Scolari, Vittore, additional, Rizzo, Arianna, additional, Beltrami, Martina, additional, Caimi, Camilla, additional, Gandolfi, Cecilia Eugenia, additional, Castaldi, Silvana, additional, Rivieccio, Bruno Alessandro, additional, Buonanno, Giacomo, additional, Marano, Giuseppe, additional, Ottomano, Cosimo, additional, Boracchi, Patrizia, additional, Biganzoli, Elia, additional, and Galli, Massimo, additional

Published
2021
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17. Seroprevalence of SARS-CoV-2 significantly varies with age: Preliminary results from a mass population screening

Author

Pagani, Gabriele, primary, Conti, Federico, additional, Giacomelli, Andrea, additional, Bernacchia, Dario, additional, Rondanin, Rossana, additional, Prina, Andrea, additional, Scolari, Vittore, additional, Gandolfi, Cecilia Eugenia, additional, Castaldi, Silvana, additional, Marano, Giuseppe, additional, Ottomano, Cosimo, additional, Boracchi, Patrizia, additional, Biganzoli, Elia, additional, and Galli, Massimo, additional

Published
2020
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20. Prevalence of SARS-CoV-2 in an Area of Unrestricted Viral Circulation: Mass Seroepidemiological Screening in Castiglione D’Adda, Italy

Author

Pagani, Gabriele, primary, Giacomelli, Andrea, additional, Conti, Federico, additional, Bernacchia, Dario, additional, Rondanin, Rossana, additional, Prina, Andrea, additional, Scolari, Vittore, additional, Rizzo, Arianna, additional, Beltrami, Martina, additional, Caimi, Camilla, additional, Gandolfi, Cecilia Eugenia, additional, Castaldi, Silvana, additional, Rivieccio, Bruno Alessandro, additional, Buonanno, Giacomo, additional, Marano, Giuseppe, additional, Ottomano, Cosimo, additional, Boracchi, Patrizia, additional, and Biganzoli, Elia, additional

Published
2020
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31. Raccomandazioni della Federazione Italiana delle Società di Medicina di Laboratorio (FISMeLab) per il trasporto del materiale biologico

Author

Miconi, Valentino, primary, Brando, Bruno, additional, Clerici, Pierangelo, additional, Crivelli, Filippo, additional, Curcio, Francesco, additional, Giardini, Roberto, additional, Magliano, Enrico, additional, Ottomano, Cosimo, additional, Stioui, Sabine, additional, Torresani, Erminio, additional, Zaninotto, Martina, additional, and Cenci, Anna Maria, additional

Published
2019
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32. Comparison between optical microscopy and automation for cytometric analysis of pericardial fluids in a cohort of adult subjects undergoing cardiac surgery

Author

Buoro, Sabrina, primary, Seghezzi, Michela, additional, Baigorria Vaca, Maria del Carmen, additional, Manenti, Barbara, additional, Moioli, Valentina, additional, Previtali, Giulia, additional, Simon, Caterina, additional, Cugola, Diego, additional, Brucato, Antonio, additional, Ottomano, Cosimo, additional, and Lippi, Giuseppe, additional

Published
2019
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34. [Physical, chemical and morphological urine examination guidelines for the Analytical Phase from the Intersociety Urinalysis Group]

Author

Manoni, Fabio, Gessoni, Gianluca, Fogazzi, Giovanni Battista, Alessio, Maria Grazia, Caleffi, Alberta, Gambaro, Giovanni, Epifani, Maria Grazia, Pieretti, Barbara, Perego, Angelo, Ottomano, Cosimo, Saccani, Graziella, Valverde, Sara, and Secchiero, Sandra

Subjects
Settore MED/14 - NEFROLOGIA, Humans, Urinalysis, Urine, Esame Urine
Abstract

With these guidelines the Intersociety Urinalysis Group (GIAU) aims to stimulate the following aspects: Improvement and standardization of the analytical approach to physical, chemical and morphological urine examination (ECMU). Improvement of the chemical analysis of urine with particular regard to the reconsideration of the diagnostic significance of the parameters that are traditionally evaluated in dipstick analysis together with an increasing awareness of the limits of sensitivity and specificity of this analytical method. Increase the awareness of the importance of professional skills in the field of urinary morphology and the relationship with the clinicians. Implement a policy of evaluation of the analytical quality by using, in addition to traditional internal and external controls, a program for the evaluation of morphological competence. Stimulate the diagnostics industry to focus research efforts and development methodology and instrumental catering on the needs of clinical diagnosis. The hope is to revalue the enormous diagnostic potential of 'ECMU, implementing a urinalysis on personalized diagnostic needs for each patient. Emphasize the value added to ECMU by automated analyzers for the study of the morphology of the corpuscular fraction urine. The hope is to revalue the enormous potential diagnostic of 'ECMU, implementing a urinalysis on personalized diagnostic needs that each patient brings with it.

Published
2017

39. Innovative haematological parameters for early diagnosis of sepsis in adult patients admitted in intensive care unit

Author

Buoro, Sabrina, primary, Manenti, Barbara, additional, Seghezzi, Michela, additional, Dominoni, Paola, additional, Barbui, Tiziano, additional, Ghirardi, Arianna, additional, Carobbio, Alessandra, additional, Marchesi, Gianmariano, additional, Riva, Ivano, additional, Nasi, Alessandra, additional, Ottomano, Cosimo, additional, and Lippi, Giuseppe, additional

Published
2017
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41. Evaluation of nucleated red blood cell count by Sysmex XE-2100 in patients with thalassaemia or sickle cell anaemia and in neonates

Author

Buoro, Sabrina, Vavassori, Mauro, Pipitone, Silvia, Benegiamo, Anna, Lochis, Eleonora, Fumagalli, Sabina, Falanga, Anna, Marchetti, Marina, Crippa, Alberto, Ottomano, Cosimo, Lippi, Giuseppe, Buoro, S, Vavassori, M, Pipitone, S, Benegiamo, A, Lochis, E, Fumagalli, S, Falanga, A, Marchetti, M, Crippa, A, Ottomano, C, and Lippi, G

Subjects
Adult, Male, analysers, Adolescent, Erythroblasts, nucleated red blood cell, analysers, haematology, Anemia, Sickle Cell, Sensitivity and Specificity, Young Adult, nucleated red blood cells, XE-2100, thalassaemia, sickle cell disease, neonates, Humans, False Positive Reactions, Fluorometry, Child, False Negative Reactions, Observer Variation, beta-Thalassemia, Infant, Newborn, Infant, Middle Aged, ROC Curve, Area Under Curve, Child, Preschool, haematology, Erythrocyte Count, nucleated red blood cell, Original Article, Female
Abstract

Background. Current haematology analysers have variable sensitivity and accuracy for counting nucleated red blood cells in samples with low values and in all those conditions characterised by altered sensitivity of red blood cells to the lysing process, such as in beta-thalassaemia or sickle-cell diseases and in neonates. The aim of our study was to evaluate the performance of the automated analyser XE-2100 at counting nucleated red blood cells in the above-mentioned three categories of subjects with potentially altered red blood cell lysis sensitivity and yet a need for accurate nucleated red blood cell counts. Materials and methods. We measured nucleated red blood cell count by XE-2100 in peripheral blood samples of 187 subjects comprising 55 patients with beta-thalassaemia (40 major and 15 traits), 26 sickle-cell patients, 56 neonates and 50 normal subject. Results were compared with those obtained by optical microscopy. Agreement between average values of the two methods was estimated by means of Pearson's correlation and bias analysis, whereas diagnostic accuracy was estimated by analysis of receiver operating characteristic curves. Results. The comparison between the two methods showed a Pearson's correlation of 0.99 (95% CI; 0.98-0.99; p

Published
2015

42. Clinical significance of cell population data (CPD) on Sysmex XN-9000 in septic patients with our without liver impairment

Author

Buoro1, Sabrina, primary, Seghezzi, Michela, additional, Vavassori, Mauro, additional, Dominoni, Paola, additional, Apassiti Esposito, Sara, additional, Manenti, Barbara, additional, Mecca, Tommaso, additional, Marchesi, Gianmariano, additional, Castellucci, Enrico, additional, Azzarà, Giovanna, additional, Ottomano, Cosimo, additional, and Lippi, Giuseppe, additional

Published
2016
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43. Optimization of Cellular analysis of Synovial Fluids by optical microscopy and automated count using the Sysmex XN Body Fluid Mode

Author

Seghezzi, Michela, primary, Buoro, Sabrina, additional, Manenti, Barbara, additional, Mecca, Tommaso, additional, Ferrari, Roberto, additional, Zappalà, Giorgio, additional, Castelli, Claudio Carlo, additional, Balboni, Fiamma, additional, Pezzati, Paola, additional, Ottomano, Cosimo, additional, and Lippi, Giuseppe, additional

Published
2016
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