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This study presents a comprehensive optimization of algal oil extraction and transesterification for sustainable biodiesel production. Freshwater Spirogyra algae underwent Soxhlet extraction using n-hexane. response surface methodology (RSM), principal component analysis (PCA), and multivariate regression analysis (MRA) were employed to investigate the effects of biomass–solvent ratio (BSR), algae particle size (APS), and extraction-contact time (E-CT) on algal oil yield (AOY). The extracted oil was then converted to biodiesel via transesterification, and the impacts of the methanol–oil ratio (MOR) and transesterification-contact time (T-CT) on biodiesel conversion efficiency (BCE) were analyzed. Results demonstrate that optimal BSR, APS, and E-CT for maximal AOY are 1:7, 400 µm, and 3–4 h, respectively. For transesterification, a MOR of 12:1 and a T-CT of 4 h yielded the highest BCE. Predictive models exhibited exceptional accuracy, with R2 values of 0.916 and 0.950 for AOY and BCE, respectively. The produced biodiesel complied with ASTM D6751 and EN 14214, showcasing its potential for renewable energy applications. [ABSTRACT FROM AUTHOR]

Various rapid propagation strategies have been discovered, which has facilitated large-scale plant reproduction and cultivar development. These methods, in many plant species, are used to rapidly generate large quantities (900 mini-tubers/m2) of high-quality propagule (free from contamination) at a relatively low cost in a small space. They are also used for plant preservation. This review article aims to provide potential applications for regeneration and clonal propagation. Plant propagation using advanced agrotechnology, such as aeroponics, is becoming increasingly popular among academics and industrialists. The advancement of asexual aeroponic propagation has been achieved through advancements in monitoring and control systems using IoT and smart sensor technology. New sensor technology systems have gained substantial interest in agriculture in recent years. It is used in agriculture to precisely arrange various operations and objectives while harnessing limited resources with minimal human intervention. Modern intelligent technologies and control systems simplify sensor data collection, making it more efficient than manual data collection, which can be slow and prone to errors. Specific ambient variables like temperature, humidity, light intensity, stock solution concentrations (nutrient water), EC (electrical conductivity), pH values, CO2 content, and atomization parameters (frequency and interval) are collected more effectively through these systems. The use of intelligent technologies provides complete control over the system. When combined with IoT, it aids in boosting crop quality and yield while also lowering production costs and providing data directly to tablets and smartphones in aeroponic propagation systems. It can potentially increase the system's productivity and usefulness compared to the older manual monitoring and operating methods. [ABSTRACT FROM AUTHOR]

Though, the performance of insecticides depends on various factors (i.e. pests, insecticide formulation, entry route, mode of action, dosage, calibration and application timing) but one factor that doesn’t get much attention is the quality of the water used to spray the product which may reflect in the success of spray operation. To know the influence of water quality on pesticide performance, this study was proposed to evaluate the water sources used for mixing the insecticides prior to application. For this purpose, two districts of lower Sindh province i.e., Hyderabad and Tando Allahyar were selected with 20 samples from each district for assessing the carrier water quality. Samples were used for the measurement of electrical conductivity (EC), total dissolved solids (TDS), pH, cations including potassium (K+), calcium (Ca2+), magnesium (Mg2+), iron (Fe2+) and sodium (Na+). In anions, bicarbonate (HCO3−), carbonate (CO32−), chloride (Cl−), nitrate (NO3-) and sulphate (SO2-) were recorded. The Durov diagram was generated which delineated that the overall water samples fell under mixed zones, therefore, the groundwater found neither anion dominant nor cation dominant, but Na-Cl type. The results further revealed that 95% of water samples (38) were alkaline in nature and unfit for dilution purposes of insecticide including Abamectin, Cyromazine, Fluvalinate, Imidacloprid, Methiocarb and Spiromesifen. Moreover, all the samples were exceeding the permissible pH level of 4.0 to 6.5 required for the mixing of commonly used insecticide such as Acephate, Azadiachtin, Buprofezin, Fenpropathrin, Fenpyroximate, Flonicamid and Pyriproxyfen. Furthermore, there were 23 samples (58%) exceeding the required level (114-342) of hardness and 39 (98%) samples were unfit in terms of TSD levels (250 ppm). The results of the study can be concluded that the water of the study area was not suitable for insecticide dilution and the regular water quality testing is mandatory. The water pH should be maintained for performance of insecticides. [ABSTRACT FROM AUTHOR]

The objective of the research work was to observe the rarely seen behavior in morphological domestic Goat (Capra aegagrushircus) have hermaphroditic condition. This case has been reported in a domestic goat (Capra aegagrushircus) having age of 3.5 years from Sunigram valley district Buner KP. The reported species having both male and female characters. The pictures have been taken from the reported species and shows clearly both male and female organs. This type of case has been reported for the first time from Pakistan. The male characters observed are having testes and scrotum. The neck morphology shows male character. The body is stout. Snout shape shows male character. Eyes show male character. The tail is erect above this also shows male character. Having a long beard. The hairs on front legs show male character. Hairs on the neck show male character. The male goat teasing the female goat but cannot mate. The female characters observed having Ears are elongated and suspended. Having two teats (mammary glands) and vegina. The belly has slope shape. Nose shows female character. Breast shows female character. Mouth is broad and shows female character. It is useful information for the Geneticists in future study. [ABSTRACT FROM AUTHOR]