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1. Raman image-activated cell sorting

Author

Nitta, Nao, Iino, Takanori, Isozaki, Akihiro, Yamagishi, Mai, Kitahama, Yasutaka, Sakuma, Shinya, Suzuki, Yuta, Tezuka, Hiroshi, Oikawa, Minoru, Arai, Fumihito, Asai, Takuya, Deng, Dinghuan, Fukuzawa, Hideya, Hase, Misa, Hasunuma, Tomohisa, Hayakawa, Takeshi, Hiraki, Kei, Hiramatsu, Kotaro, Hoshino, Yu, Inaba, Mary, Inoue, Yuki, Ito, Takuro, Kajikawa, Masataka, Karakawa, Hiroshi, Kasai, Yusuke, Kato, Yuichi, Kobayashi, Hirofumi, Lei, Cheng, Matsusaka, Satoshi, Mikami, Hideharu, Nakagawa, Atsuhiro, Numata, Keiji, Ota, Tadataka, Sekiya, Takeichiro, Shiba, Kiyotaka, Shirasaki, Yoshitaka, Suzuki, Nobutake, Tanaka, Shunji, Ueno, Shunnosuke, Watarai, Hiroshi, Yamano, Takashi, Yazawa, Masayuki, Yonamine, Yusuke, Di Carlo, Dino, Hosokawa, Yoichiroh, Uemura, Sotaro, Sugimura, Takeaki, Ozeki, Yasuyuki, and Goda, Keisuke

Published
2020
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2. Raman image-activated cell sorting

Author

Nitta, Nao, Iino, Takanori, Isozaki, Akihiro, Yamagishi, Mai, Kitahama, Yasutaka, Sakuma, Shinya, Suzuki, Yuta, Tezuka, Hiroshi, Oikawa, Minoru, Arai, Fumihito, Asai, Takuya, Deng, Dinghuan, Fukuzawa, Hideya, Hase, Misa, Hasunuma, Tomohisa, Hayakawa, Takeshi, Hiraki, Kei, Hiramatsu, Kotaro, Hoshino, Yu, Inaba, Mary, Inoue, Yuki, Ito, Takuro, Kajikawa, Masataka, Karakawa, Hiroshi, Kasai, Yusuke, Kato, Yuichi, Kobayashi, Hirofumi, Lei, Cheng, Matsusaka, Satoshi, Mikami, Hideharu, Nakagawa, Atsuhiro, Numata, Keiji, Ota, Tadataka, Sekiya, Takeichiro, Shiba, Kiyotaka, Shirasaki, Yoshitaka, Suzuki, Nobutake, Tanaka, Shunji, Ueno, Shunnosuke, Watarai, Hiroshi, Yamano, Takashi, Yazawa, Masayuki, Yonamine, Yusuke, Di, Carlo Dino, Hosokawa, Yoichiroh, Uemura, Sotaro, Sugimura, Takeaki, Ozeki, Yasuyuki, Goda, Keisuke, Nitta, Nao, Iino, Takanori, Isozaki, Akihiro, Yamagishi, Mai, Kitahama, Yasutaka, Sakuma, Shinya, Suzuki, Yuta, Tezuka, Hiroshi, Oikawa, Minoru, Arai, Fumihito, Asai, Takuya, Deng, Dinghuan, Fukuzawa, Hideya, Hase, Misa, Hasunuma, Tomohisa, Hayakawa, Takeshi, Hiraki, Kei, Hiramatsu, Kotaro, Hoshino, Yu, Inaba, Mary, Inoue, Yuki, Ito, Takuro, Kajikawa, Masataka, Karakawa, Hiroshi, Kasai, Yusuke, Kato, Yuichi, Kobayashi, Hirofumi, Lei, Cheng, Matsusaka, Satoshi, Mikami, Hideharu, Nakagawa, Atsuhiro, Numata, Keiji, Ota, Tadataka, Sekiya, Takeichiro, Shiba, Kiyotaka, Shirasaki, Yoshitaka, Suzuki, Nobutake, Tanaka, Shunji, Ueno, Shunnosuke, Watarai, Hiroshi, Yamano, Takashi, Yazawa, Masayuki, Yonamine, Yusuke, Di, Carlo Dino, Hosokawa, Yoichiroh, Uemura, Sotaro, Sugimura, Takeaki, Ozeki, Yasuyuki, and Goda, Keisuke

Abstract

The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.

Published
2023

3. Intelligent image-activated cell sorting 2.0

Author

Isozaki, Akihiro, primary, Mikami, Hideharu, additional, Tezuka, Hiroshi, additional, Matsumura, Hiroki, additional, Huang, Kangrui, additional, Akamine, Marino, additional, Hiramatsu, Kotaro, additional, Iino, Takanori, additional, Ito, Takuro, additional, Karakawa, Hiroshi, additional, Kasai, Yusuke, additional, Li, Yan, additional, Nakagawa, Yuta, additional, Ohnuki, Shinsuke, additional, Ota, Tadataka, additional, Qian, Yong, additional, Sakuma, Shinya, additional, Sekiya, Takeichiro, additional, Shirasaki, Yoshitaka, additional, Suzuki, Nobutake, additional, Tayyabi, Ehsen, additional, Wakamiya, Tsubasa, additional, Xu, Muzhen, additional, Yamagishi, Mai, additional, Yan, Haochen, additional, Yu, Qiang, additional, Yan, Sheng, additional, Yuan, Dan, additional, Zhang, Wei, additional, Zhao, Yaqi, additional, Arai, Fumihito, additional, Campbell, Robert E., additional, Danelon, Christophe, additional, Di Carlo, Dino, additional, Hiraki, Kei, additional, Hoshino, Yu, additional, Hosokawa, Yoichiroh, additional, Inaba, Mary, additional, Nakagawa, Atsuhiro, additional, Ohya, Yoshikazu, additional, Oikawa, Minoru, additional, Uemura, Sotaro, additional, Ozeki, Yasuyuki, additional, Sugimura, Takeaki, additional, Nitta, Nao, additional, and Goda, Keisuke, additional

Published
2020
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4. Intelligent Image-Activated Cell Sorting

Author

30183924, 00581323, 70570167, Nitta, Nao, Sugimura, Takeaki, Isozaki, Akihiro, Mikami, Hideharu, Hiraki, Kei, Sakuma, Shinya, Iino, Takanori, Arai, Fumihito, Endo, Taichiro, Fujiwaki, Yasuhiro, Fukuzawa, Hideya, Hase, Misa, Hayakawa, Takeshi, Hiramatsu, Kotaro, Hoshino, Yu, Inaba, Mary, Ito, Takuro, Karakawa, Hiroshi, Kasai, Yusuke, Koizumi, Kenichi, Lee, SangWook, Lei, Cheng, Li, Ming, Maeno, Takanori, Matsusaka, Satoshi, Murakami, Daichi, Nakagawa, Atsuhiro, Oguchi, Yusuke, Oikawa, Minoru, Ota, Tadataka, Shiba, Kiyotaka, Shintaku, Hirofumi, Shirasaki, Yoshitaka, Suga, Kanako, Suzuki, Yuta, Suzuki, Nobutake, Tanaka, Yo, Tezuka, Hiroshi, Toyokawa, Chihana, Yalikun, Yaxiaer, Yamada, Makoto, Yamagishi, Mai, Yamano, Takashi, Yasumoto, Atsushi, Yatomi, Yutaka, Yazawa, Masayuki, Di Carlo, Dino, Hosokawa, Yoichiroh, Uemura, Sotaro, Ozeki, Yasuyuki, Goda, Keisuke, 30183924, 00581323, 70570167, Nitta, Nao, Sugimura, Takeaki, Isozaki, Akihiro, Mikami, Hideharu, Hiraki, Kei, Sakuma, Shinya, Iino, Takanori, Arai, Fumihito, Endo, Taichiro, Fujiwaki, Yasuhiro, Fukuzawa, Hideya, Hase, Misa, Hayakawa, Takeshi, Hiramatsu, Kotaro, Hoshino, Yu, Inaba, Mary, Ito, Takuro, Karakawa, Hiroshi, Kasai, Yusuke, Koizumi, Kenichi, Lee, SangWook, Lei, Cheng, Li, Ming, Maeno, Takanori, Matsusaka, Satoshi, Murakami, Daichi, Nakagawa, Atsuhiro, Oguchi, Yusuke, Oikawa, Minoru, Ota, Tadataka, Shiba, Kiyotaka, Shintaku, Hirofumi, Shirasaki, Yoshitaka, Suga, Kanako, Suzuki, Yuta, Suzuki, Nobutake, Tanaka, Yo, Tezuka, Hiroshi, Toyokawa, Chihana, Yalikun, Yaxiaer, Yamada, Makoto, Yamagishi, Mai, Yamano, Takashi, Yasumoto, Atsushi, Yatomi, Yutaka, Yazawa, Masayuki, Di Carlo, Dino, Hosokawa, Yoichiroh, Uemura, Sotaro, Ozeki, Yasuyuki, and Goda, Keisuke

Abstract

A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.

Published
2018

5. Intelligent Image-Activated Cell Sorting

Author

Nitta, Nao, primary, Sugimura, Takeaki, additional, Isozaki, Akihiro, additional, Mikami, Hideharu, additional, Hiraki, Kei, additional, Sakuma, Shinya, additional, Iino, Takanori, additional, Arai, Fumihito, additional, Endo, Taichiro, additional, Fujiwaki, Yasuhiro, additional, Fukuzawa, Hideya, additional, Hase, Misa, additional, Hayakawa, Takeshi, additional, Hiramatsu, Kotaro, additional, Hoshino, Yu, additional, Inaba, Mary, additional, Ito, Takuro, additional, Karakawa, Hiroshi, additional, Kasai, Yusuke, additional, Koizumi, Kenichi, additional, Lee, SangWook, additional, Lei, Cheng, additional, Li, Ming, additional, Maeno, Takanori, additional, Matsusaka, Satoshi, additional, Murakami, Daichi, additional, Nakagawa, Atsuhiro, additional, Oguchi, Yusuke, additional, Oikawa, Minoru, additional, Ota, Tadataka, additional, Shiba, Kiyotaka, additional, Shintaku, Hirofumi, additional, Shirasaki, Yoshitaka, additional, Suga, Kanako, additional, Suzuki, Yuta, additional, Suzuki, Nobutake, additional, Tanaka, Yo, additional, Tezuka, Hiroshi, additional, Toyokawa, Chihana, additional, Yalikun, Yaxiaer, additional, Yamada, Makoto, additional, Yamagishi, Mai, additional, Yamano, Takashi, additional, Yasumoto, Atsushi, additional, Yatomi, Yutaka, additional, Yazawa, Masayuki, additional, Di Carlo, Dino, additional, Hosokawa, Yoichiroh, additional, Uemura, Sotaro, additional, Ozeki, Yasuyuki, additional, and Goda, Keisuke, additional

Published
2018
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6. Intelligent Cell Search Engine

Author

Nitta, Nao, primary, Sugimura, Takeaki, additional, Isozaki, Akihiro, additional, Mikami, Hideharu, additional, Hiraki, Kei, additional, Sakuma, Shinya, additional, Iino, Takanori, additional, Arai, Fumihito, additional, Endo, Taichiro, additional, Fujiwaki, Yasuhiro, additional, Fukuzawa, Hideya, additional, Hase, Misa, additional, Hayakawa, Takeshi, additional, Hiramatsu, Kotaro, additional, Hoshino, Yu, additional, Inaba, Mary, additional, Ito, Takuro, additional, Karakawa, Hiroshi, additional, Kasai, Yusuke, additional, Koizumi, Kenichi, additional, Lee, SangWook, additional, Lei, Cheng, additional, Li, Ming, additional, Maeno, Takanori, additional, Matsusaka, Satoshi, additional, Murakami, Daichi, additional, Nakagawa, Atsuhiro, additional, Oguchi, Yusuke, additional, Oikawa, Minoru, additional, Ota, Tadataka, additional, Shiba, Kiyotaka, additional, Shintaku, Hirofumi, additional, Shirasaki, Yoshitaka, additional, Suga, Kanako, additional, Suzuki, Yuta, additional, Suzuki, Nobutake, additional, Tanaka, Yo, additional, Tezuka, Hiroshi, additional, Toyokawa, Chihana, additional, Yalikun, Yaxiaer, additional, Yamada, Makoto, additional, Yamagishi, Mai, additional, Yamano, Takashi, additional, Yasumoto, Atsushi, additional, Yatomi, Yutaka, additional, Yazawa, Masayuki, additional, Di Carlo, Dino, additional, Hosokawa, Yoichiroh, additional, Uemura, Sotaro, additional, Ozeki, Yasuyuki, additional, and Goda, Keisuke, additional

Published
2018
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