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Your search keyword '"Osteoprotegerin/metabolism"' showing total 2 results
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2 results on '"Osteoprotegerin/metabolism"'

1. Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis

Author

Anja Schlagenhauf, Katrin S. Lips, Hans-Joachim Wilke, Wolfgang Böcker, Stefanie Kern, Fathi Hassan, Anita Ignatius, Sabine Stoetzel, Deeksha Malhan, Angela Rösen-Wolff, Christian Heiss, Felix Merboth, Dirk Rosenbaum, Felix Schulze, Natali Bauer, Judith Langenstein, Thaqif El Khassawna, Dirk Hose, Anja Secklinger, Diaa Eldin S. Daghma, Markus Rupp, Hematology, and Basic (bio-) Medical Sciences

Subjects
0301 basic medicine, Bone density, Osteoporosis, Methylprednisolone/pharmacology, surgery, chemistry.chemical_compound, 0302 clinical medicine, Bone Density, Signal Transduction/drug effects, Osteoporosis/metabolism, Orthopedics and Sports Medicine, Osteoprotegerin/metabolism, biology, hematology, NF-kappa B, RANK Ligand/metabolism, medicine.anatomical_structure, RANKL, Osteocyte, Female, Spine/drug effects, Signal Transduction, musculoskeletal diseases, medicine.medical_specialty, Ovariectomy, 030209 endocrinology & metabolism, Methylprednisolone, Osteocytes, Pathology and Forensic Medicine, 03 medical and health sciences, Osteoprotegerin, Internal medicine, medicine, Internal Medicine, Animals, Sheep, business.industry, RANK Ligand, NF-κB, Bone Density/drug effects, medicine.disease, Spine, Disease Models, Animal, 030104 developmental biology, Endocrinology, chemistry, NF-kappa B/metabolism, biology.protein, Sclerostin, business, Osteocytes/drug effects
Abstract

Osteoporosis induction in a sheep model by steroid administration combined with ovariectomy recapitulates decreased bone formation and substandard matrix mineralization in patients. Recently, the role of osteocytes has been frequently addressed, with focus on their role in osteoclastogenesis. However, the quantification of receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) signaling in osteocytes was not studied in sheep. The current study reproduced the sheep model of osteoporosis to study the RANKL/OPG ratio correlation to the method of osteoporosis induction. We investigated the induction of osteoporosis after 8 months using 31 female merino land sheep divided into four groups: control, ovariectomy, ovariectomy with dietary limitation, and ovariectomy with dietary limitation and steroid injection. In accordance to previous reports, the present study showed trabecular thinning, higher numbers of apoptotic osteocytes, and imbalanced metabolism, leading to defective mineralization. The global RANKL/OPG ratio in the spine after 8 months of steroid and dietary treatment was not different from that of the control. Interestingly, assessment of the osteocyte-specific RANKL/OPG ratio showed that the steroid-induced osteoporosis in its late progressive phase stimulates RANKL expression in osteocytes. Sclerostin is suggested to induce RANKL expression in osteocytes. The findings of this study can contribute to further explain the success of sclerostin antibodies in treating osteoporotic patients despite increased osteocyte-expressed RANKL.

Published
2017
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2. Low-dose prednisolone in early rheumatoid arthritis inhibits collagen type I degradation by matrix metalloproteinases as assessed by serum 1CTP-a possible mechanism for specific inhibition of radiological destruction

Author

Engvall, I.L., Svensson, B., Boonen, A., Heijde, D. van der, Lerner, U.H., Hafstrom, I., BARFOT Study Grp, Rheumatology, Interne Geneeskunde, and RS: CAPHRI School for Public Health and Primary Care

Subjects
Male, rheumatoid arthritis, Peptides/blood, CTX-1, Osteoclasts, Arthritis, Collagen Type I/antagonists & inhibitors, Matrix metalloproteinase, Arthritis, Rheumatoid, Pharmacology (medical), Prospective Studies, 1CTP, Osteoprotegerin/metabolism, Medicine(all), biology, Remission Induction, RANKL, prednisolone, Middle Aged, RANK Ligand/metabolism, Rheumatoid arthritis, Prednisolone, Disease Progression, young adult, Female, joint destruction, bone resorption, radiography, medicine.drug, musculoskeletal diseases, Adult, Prednisolone/administration & dosage, medicine.medical_specialty, Adolescent, Collagen Type I, Bone resorption, Rheumatology, Osteoprotegerin, Internal medicine, medicine, Humans, Glucocorticoids, Aged, business.industry, RANK Ligand, Glucocorticoids/administration & dosage, medicine.disease, Matrix Metalloproteinases, Endocrinology, Matrix Metalloproteinases/metabolism, biology.protein, Osteoclasts/drug effects, OPG, Peptides, business, aged, 80 and over, Arthritis, Rheumatoid/diagnostic imaging, Follow-Up Studies
Abstract

Objective. To study the effects of low-dose prednisolone on the osteoclast-regulating proteins osteoprotegerin (OPG) and RANK ligand (RANKL) and on markers of bone resorption, 1CTP generated by MMPs and CTX-1 generated by cathepsin K, in patients with early RA in relation to inflammation and joint destruction. Methods. In 225 patients, who at the start of the first DMARD had been randomized to 7.5 mg prednisolone daily for 2 years, the P-group, or no prednisolone, the NoP-group, OPG and RANKL were analysed at 0-24 months and 1CTP and CTX-1 at 0-12 months. Radiographs of hands and feet were assessed at 0, 1 and 2 years using the modified Sharp-van der Heijde score and radiological progression defined as increase in total Sharp score above 5.8. Data were analysed with a mixed linear model and by the GENMOD procedure. Results. In the P-group, RANKL and the ratio OPG/RANKL were stable between baseline and 24 months, whereas in the NoP-group, RANKL increased and the ratio OPG/RANKL decreased. CTX-1 decreased significantly more in the P-group. 1CTP decreased over time in both groups, but more in the P-group, P

Published
2013

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