1. Chemokine Receptors CXCR3 and CCR6 and Their Ligands in the Liver and Blood of Patients with Chronic Hepatitis C.
- Author
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Arsent'eva NA, Semenov AV, Lyubimova NE, Ostankov YV, Elezo DS, Kudryavtsev IV, Basina VV, Esaulenko EV, Kozlov KV, Zhdanov KV, and Totolyan AA
- Subjects
- Disease Progression, Female, Gene Expression Profiling, Gene Expression Regulation, Hepatitis C, Chronic blood, Hepatitis C, Chronic pathology, Humans, Ligands, Liver Cirrhosis blood, Liver Cirrhosis diagnosis, Liver Cirrhosis etiology, Lymphocytes immunology, Male, Receptors, Chemokine blood, Receptors, Chemokine genetics, Receptors, Chemokine metabolism, Hepatitis C, Chronic diagnosis, Receptors, CCR6 blood, Receptors, CCR6 metabolism, Receptors, CXCR3 blood, Receptors, CXCR3 metabolism
- Abstract
We performed a comprehensive analysis of CCR6 and CXCR3 chemokine receptors and their ligands CCL20/MIP-3α, CXCL9/MIG, CXCL10/IP-10, and CXCL11/ITAC in the liver and blood of patients with chronic hepatitis C at different stages of the disease. TaqMan PCR was used to determine mRNA gene expression of chemokines and their receptors in liver specimens, xMAP multiplex analysis was performed to estimate the concentration of chemokines in blood plasma, and fl ow cytofluorometry was used to evaluate CCR6 and CXCR3 expression on peripheral blood lymphocyte populations. In the liver of patients with hepatitis C, mRNA expression of CXCL10, CCR6, and CXCR3 genes increases with fibrosis progression in the liver tissue. In the plasma, concentrations of all studied chemokines increased depending on the stage of liver fibrosis, CCR6 and CXCR3 expression was changed in various lymphocyte populations. Thus, chemokines are involved in the immunopathogenesis and fibrogenesis in chronic viral hepatitis C. The results suggest using these chemokines in the diagnosis and prognosis of the disease.
- Published
- 2015
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