31 results on '"Oses, R."'
Search Results
2. Tracing low‐CO2 fluxes in soil incubation and 13C labeling experiments: A simplified gas sampling system for respiration and photosynthesis measurements
- Author
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Witzgall, K., primary, Hesse, B. D., additional, Seguel, O., additional, Oses, R., additional, Grams, T. E. E., additional, and Mueller, C. W., additional
- Published
- 2023
- Full Text
- View/download PDF
3. Trends in planetary science research in the Puna and Atacama Desert regions: Underrepresentation of local scientific institutions?
- Author
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Tavernier, A., primary, Pinto, G. A., additional, Valenzuela, M., additional, Garcia, A., additional, Ulloa, C., additional, Oses, R., additional, and Foing, B. H., additional
- Published
- 2023
- Full Text
- View/download PDF
4. An improved method for intracellular DNA (iDNA) recovery from terrestrial environments
- Author
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Medina Caro, D., Horstmann, L., Ganzert, L., Oses, R., Friedl, T., and Wagner, D.
- Abstract
The simultaneous extraction of intracellular DNA (iDNA) and extracellular DNA (eDNA) can help to separate the living in situ community (represented by iDNA) from background DNA that originated both from past communities and from allochthonous sources. As iDNA and eDNA extraction protocols require separating cells from the sample matrix, their DNA yields are generally lower than direct methods that lyse the cells within the sample matrix. We, therefore, tested different buffers with and without adding a detergent mix (DM) in the extraction protocol to improve the recovery of iDNA from surface and subsurface samples that covered a variety of terrestrial environments. The combination of a highly concentrated sodium phosphate buffer plus DM significantly improved iDNA recovery for almost all tested samples. Additionally, the combination of sodium phosphate and EDTA improved iDNA recovery in most of the samples and even allowed the successful extraction of iDNA from extremely low-biomass iron-bearing rock samples taken from the deep biosphere. Based on our results, we recommend using a protocol with sodium phosphate in combination with either a DM (NaP 300 mM + DM) or EDTA (NaP + EDTA 300 mM). Furthermore, for studies that rely on the eDNA pool, we recommend using buffers solely based on sodium phosphate because the addition of EDTA or a DM resulted in a decrease in eDNA for most of the tested samples. These improvements can help reduce community bias in environmental studies and contribute to better characterizations of both modern and past ecosystems.
- Published
- 2023
5. Tracing Low‐CO2 Fluxes in Soil Incubation and 13C Labeling Experiments: A Simplified Gas Sampling System for Respiration and Photosynthesis Measurements.
- Author
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Witzgall, K., Hesse, B. D., Seguel, O., Oses, R., Grams, T. E. E., and Mueller, C. W.
- Subjects
RESPIRATORY measurements ,SOIL air ,ARID soils ,SOIL respiration ,CARBON cycle - Abstract
Quantifying carbon dioxide (CO2) fluxes between soil and atmosphere is key in understanding net ecosystem C exchange and biogeochemical C cycling in plant‐soil systems. In ecosystems with low primary production and sparse vegetation, for example, dry lands or subpolar regions where C fluxes are small, measurement sensitivity is key—even so when measurements are combined with isotopic labeling. Here, we present a simplified gas sampling system developed to facilitate sampling and measurement of low soil CO2 fluxes as well as in situ 13CO2 labeling in the same setup. The capacity of the system was tested in a set of feature tests along with gas measurements of dryland soil‐biocrust systems. The system's sensitivity to capture minor changes in CO2 concentration was confirmed in respiration and photosynthesis measurements of soil‐biocrust systems, where fluxes down to 0.1 μmol CO2 m−2 s−1 were quantified. A balloon, implemented to counterbalance underpressure build‐up during gas withdrawal, mitigated 72% of pressure differences at sampling. The overall system volume was reduced to a minimum to limit contamination caused by residual air, and the design enabled one‐step flushing and evacuation of system compartments and gas sample bags, successfully ruling out cross‐contamination between samples. Ultimately, this system offers a flexible and accessible solution for CO2 measurements that can be applied not only on arid soils with low biological activity and turnover rates, but also on plant‐soil systems. The modifications enabled larger, and thereby more representative, sample volumes to be collected while limiting incubation, contamination, and pressure effects on the intact soil system. Plain Language Summary: Measurements of carbon dioxide (CO2) fluxes between soil and atmosphere are crucial to understand the terrestrial carbon cycle. In dry regions, these fluxes are rather small as both the release of CO2 via microbial respiration and uptake of CO2 via photosynthesis is limited by lack of water and sparse vegetation cover. We have developed a simplified CO2 gas sampling setup suitable for incubation experiments of soils with low biological activity and turnover rates, for example, soils from dry lands or subpolar regions, using only cost‐effective, easily available and replaceable system compartments. We tested system parameters in a set of feature tests and could rule out leakage and cross‐contamination. We further confirmed the suitability of the system for capturing small CO2 fluxes in photosynthesis and respiration measurements of soils with biocrusts. A balloon was installed to counterbalance pressure build‐up during sampling, allowing for larger gas volumes to be collected, which resulted in a mitigation of 72% of the underpressure build‐up. This system offers a straightforward and accessible solution for CO2 measurements, with features limiting contamination and pressure effects on the research sample. Key Points: We present a simplified and low‐cost gas sampling system, developed for quantifying CO2 fluxes in soil incubation experimentsRespiration rates down to 0.1 μmol CO2 m−2 s−1 were quantified, confirming the suitability of the system for low‐CO2 flux measurementsA balloon was installed, mitigating 72% of pressure changes in the headspace during sampling [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
6. Data supplement to 'Pedogenic and microbial interrelations to regional climate and local topography: New insights from a climate gradient (arid to humid) along the Coastal Cordillera of Chile'
- Author
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Bernhard, N., Moskwa, L., Schmidt, K., Oeser, R., Aburto, F., Bader, M., Baumann, K., von Blanckenburg, F., Boy, J., van den Brink, L., Brucker, E., Büdel, B., Canessa, R., Dippold, M., Ehlers, T., Fuentes, J., Godoy, R., Jung, P., Karsten, U., Köster, M., Kuzyakov, Y., Leinweber, P., Neidhardt, H., Matus, F., Mueller, C., Oelmann, Y., Oses, R., Osses, P., Paulino, L., Samolov, E., Schaller, M., Schmid, M., Spielvogel, S., Spohn, M., Stock, S., Stroncik, N., Tielbörger, K., Übernickel, K., Scholten, T., Seguel, O., Wagner, D., and Kühn, P.
- Subjects
ComputingMethodologies_PATTERNRECOGNITION ,Data_MISCELLANEOUS - Abstract
Dataset
- Published
- 2018
7. Tracing Low‐CO2Fluxes in Soil Incubation and 13C Labeling Experiments: A Simplified Gas Sampling System for Respiration and Photosynthesis Measurements
- Author
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Witzgall, K., Hesse, B. D., Seguel, O., Oses, R., Grams, T. E. E., and Mueller, C. W.
- Abstract
Quantifying carbon dioxide (CO2) fluxes between soil and atmosphere is key in understanding net ecosystem C exchange and biogeochemical C cycling in plant‐soil systems. In ecosystems with low primary production and sparse vegetation, for example, dry lands or subpolar regions where C fluxes are small, measurement sensitivity is key—even so when measurements are combined with isotopic labeling. Here, we present a simplified gas sampling system developed to facilitate sampling and measurement of low soil CO2fluxes as well as in situ 13CO2labeling in the same setup. The capacity of the system was tested in a set of feature tests along with gas measurements of dryland soil‐biocrust systems. The system's sensitivity to capture minor changes in CO2concentration was confirmed in respiration and photosynthesis measurements of soil‐biocrust systems, where fluxes down to 0.1 μmol CO2m−2s−1were quantified. A balloon, implemented to counterbalance underpressure build‐up during gas withdrawal, mitigated 72% of pressure differences at sampling. The overall system volume was reduced to a minimum to limit contamination caused by residual air, and the design enabled one‐step flushing and evacuation of system compartments and gas sample bags, successfully ruling out cross‐contamination between samples. Ultimately, this system offers a flexible and accessible solution for CO2measurements that can be applied not only on arid soils with low biological activity and turnover rates, but also on plant‐soil systems. The modifications enabled larger, and thereby more representative, sample volumes to be collected while limiting incubation, contamination, and pressure effects on the intact soil system. Measurements of carbon dioxide (CO2) fluxes between soil and atmosphere are crucial to understand the terrestrial carbon cycle. In dry regions, these fluxes are rather small as both the release of CO2via microbial respiration and uptake of CO2via photosynthesis is limited by lack of water and sparse vegetation cover. We have developed a simplified CO2gas sampling setup suitable for incubation experiments of soils with low biological activity and turnover rates, for example, soils from dry lands or subpolar regions, using only cost‐effective, easily available and replaceable system compartments. We tested system parameters in a set of feature tests and could rule out leakage and cross‐contamination. We further confirmed the suitability of the system for capturing small CO2fluxes in photosynthesis and respiration measurements of soils with biocrusts. A balloon was installed to counterbalance pressure build‐up during sampling, allowing for larger gas volumes to be collected, which resulted in a mitigation of 72% of the underpressure build‐up. This system offers a straightforward and accessible solution for CO2measurements, with features limiting contamination and pressure effects on the research sample. We present a simplified and low‐cost gas sampling system, developed for quantifying CO2fluxes in soil incubation experimentsRespiration rates down to 0.1 μmol CO2m−2s−1were quantified, confirming the suitability of the system for low‐CO2flux measurementsA balloon was installed, mitigating 72% of pressure changes in the headspace during sampling We present a simplified and low‐cost gas sampling system, developed for quantifying CO2fluxes in soil incubation experiments Respiration rates down to 0.1 μmol CO2m−2s−1were quantified, confirming the suitability of the system for low‐CO2flux measurements A balloon was installed, mitigating 72% of pressure changes in the headspace during sampling
- Published
- 2023
- Full Text
- View/download PDF
8. Fungal endophytes in xylem of healthy chilean trees and their possible role in early wood decay
- Author
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Freer-Calderón, Juanita, Oses, R., Rodríguez, J., Sanfuentes-Von Stowasser, Eugenio, and Valenzuela, S.
- Abstract
A survey of fungal endophytes associated with xylem of presumably healthy trees was conducted. Wood-inhabiting fungal endophytes of Prumnopitys andina, Podocarpus saligna, Drimys winteri and Nothofagus obliqua were isolated from surface sterilized xylem core samples. Five basidiomycetes (Inonotus sp., Bjerkandra adusta and three unknown strains), two ascomycetes (Xylaria sp., Bipolaris sp.) and one anamorphic strain were detected. Xylaria sp and Bjerkandera adusta were the most frequent fungal isolates. Ultrastructural observations of wood cores samples by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and light microscopy showed the presence of fungal hyphae attached to the inner Cell surface inhabiting xylem elements even before the induction of wood degradation. Evidence of latent infection was found mainly along the parenchyma rays indicating, Fungal colonization and distribution. Results Showed simultaneous decay of all Wood components, characterized by a thinning cell-wall from the cell lumen to the Middle lamella and erosive wood degradation typical of non selective white rot. By using SEM, TEM and light microscopy it was possible to detect natural incidence of latent infections, to visualize spreading of colonization and the ability to produce wood degradation Under suitable conditions.
- Published
- 2008
9. Andrology (Male Fertility, Spermatogenesis)
- Author
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Matsumoto, Y., primary, Goto, S., additional, Hashimoto, H., additional, Kokeguchi, S., additional, Shiotani, M., additional, Okada, H., additional, Cohen - Bacrie, P., additional, Hazout, A., additional, Belloc, S., additional, De Mouzon, J., additional, Menezo, Y., additional, Dumont, M., additional, Junca, A. M., additional, Cohen-Bacrie, M., additional, Alvarez, S., additional, Olivennes, F., additional, Prisant, N., additional, Weltin, M., additional, Geissler, W., additional, Clussmann, C., additional, Strowitzki, T., additional, Eggert-Kruse, W., additional, Endou, Y., additional, Fjii, Y., additional, Motoyama, H., additional, Quintana, F. Q., additional, Zaloa Larreategui, Z. L., additional, Iratxe Penalba, I. P., additional, Sara Ortega, S. O., additional, Monica Martin, M. M., additional, Guillermo Quea, G. Q., additional, Jose Serna, J. S., additional, Showell, M. G., additional, Brown, J., additional, Yazdani, A., additional, Stankiewicz, M. T., additional, Hart, R. J., additional, Zumoffen, C., additional, Munuce, M. J., additional, Caille, A., additional, Ghersevich, S., additional, Lendinez, A. M., additional, Perez-Nevot, B., additional, Palomares, A. R., additional, Serrano Garballo, A., additional, Rodriguez, A., additional, Reche, A., additional, Mayor-Olea, A., additional, Ruiz-Galdon, M., additional, Reyes-Engel, A., additional, Mendiola, J., additional, Jorgensen, N., additional, Andersson, A. M., additional, Calafat, A. M., additional, Redmon, J. B., additional, Drobnis, E. Z., additional, Wang, C., additional, Sparks, A., additional, Thurston, S. W., additional, Liu, F., additional, Swan, S. H., additional, Tarasconi, A. C., additional, Tarasconi, B. V., additional, Tarasconi, D. V., additional, Silva, E. M. V., additional, Fujii, Y., additional, Crha, I., additional, Pribyl, J., additional, Skladal, P., additional, Zakova, J., additional, Ventruba, P., additional, Pohanka, M., additional, De La Fuente, G., additional, Pacheco, A., additional, Velasco, J. A. G., additional, Requena, A., additional, Pacheco Castro, A., additional, San Celestino Carchenilla, M., additional, Salvanes, R., additional, Arnanz, A., additional, Balmori, C., additional, Pellicer, A., additional, Garcia-Velasco, J. A., additional, Ishikawa, T., additional, Fujisawa, M., additional, Kranz, S., additional, Hersemeyer, K., additional, Hentrich, A., additional, Tinneberg, H. R., additional, Konrad, L., additional, Simon, L., additional, Lutton, D., additional, McManus, J., additional, Lewis, S. E. M., additional, Rubio, S., additional, Simon Sanjurjo, P., additional, Lewis, S., additional, Buzzi, J., additional, Valcarcel, A., additional, Lombardi, E., additional, Oses, R., additional, Rawe, V., additional, Young, E., additional, Magendzo, A., additional, Lizama, S., additional, Duque, G., additional, Mackenna, A., additional, Monqaut, A., additional, Zavaleta, C., additional, Lopez, G., additional, Lafuente, R., additional, Brassesco, M., additional, Condorelli, R., additional, La Vignera, S., additional, La Rosa, S., additional, Barone, N., additional, Vicari, E., additional, Bellanca, S., additional, D'Agata, R., additional, Calogero, A. E., additional, Enciso, M., additional, Iglesias, M., additional, Galan, I., additional, Gosalvez, A., additional, Gosalvez, J., additional, Curaba, M., additional, Poels, J., additional, Van Langendonckt, A., additional, Donnez, J., additional, Wyns, C., additional, Garcez, M., additional, Salvador, M., additional, Pasqualotto, E. B., additional, Braga, D. P. A. F., additional, Borges, E., additional, Pasqualotto, F. F., additional, Aoki, T., additional, Figueira, R. C. S., additional, Maldonado, L. G. L., additional, Iaconelli, A., additional, Frassini, R., additional, Mandelli, J., additional, Setti, A. S., additional, Cortezzi, S. S., additional, Di Mauro, M., additional, Burrello, N., additional, Kashir, J., additional, Jones, C., additional, Young, C., additional, Ruas, M., additional, Grasa, P., additional, Rietdorf, K., additional, Heytens, E., additional, Heindryckx, B., additional, Yoon, S. Y., additional, Fissore, R. A., additional, Deane, C. M., additional, Nikiforaki, D., additional, Tee, S. T., additional, de Sutter, P., additional, Parrington, J., additional, Coward, K., additional, Visser, L., additional, Westerveld, G. H., additional, van Daalen, S. K. M., additional, van der Veen, F., additional, Lombardi, M. P., additional, Repping, S., additional, Cubillos, S., additional, Sanchez, S., additional, Pedraza, J., additional, Charria, G., additional, Aparicio, H., additional, Gongora, A., additional, Caldino, F., additional, Cuneo, S., additional, Ou, J. P., additional, Zhao, W. E., additional, Liu, Y. F., additional, Xu, Y. W., additional, Zhou, C. Q., additional, Al-Asmar Pinar, N., additional, Peinado, V., additional, Gruhn, J., additional, Susiarjo, M., additional, Gil-Salom, M., additional, Martinez-Jabaloyas, J. M., additional, Remohi, J., additional, Rubio, C., additional, Hassold, T., additional, Al-Asmar, N., additional, Rodrigo, L., additional, Hassold, T. J., additional, Bungum, M., additional, Forsell, N., additional, Giwercman, A., additional, Amiri, I., additional, Sheikh, N., additional, Najafi, R., additional, Godarzi, M., additional, Farimani, M., additional, Makukh, H., additional, Tyrkus, M., additional, Zastavna, D., additional, Nakonechnuy, A., additional, Khayat, S. S., additional, Schileiko, L. V., additional, Kurilo, L. F., additional, Garcia-Herrero, S., additional, Garrido, N., additional, Martinez-Conejero, J. A., additional, Romany, L., additional, Meseguer, M., additional, Dorphin, B., additional, Lefevre, M., additional, Gout, C., additional, Oger, P., additional, Yazbeck, C., additional, Rougier, N., additional, De Stefani, S., additional, Scala, V., additional, Benedetti, S., additional, Tagliamonte, M. C., additional, Zavagnini, E., additional, Palini, S., additional, Bulletti, C., additional, Canestrari, F., additional, Subiran, N., additional, Pinto, F. M., additional, Candenas, M. L., additional, Agirregoitia, E., additional, Irazusta, J., additional, Cha, E. M., additional, Lee, J. H., additional, Park, I. H., additional, Lee, K. H., additional, Kim, M. H., additional, Jensen, M. S., additional, Rebordosa, C., additional, Thulstrup, A. M., additional, Toft, G., additional, Sorensen, H. T., additional, Bonde, J. P., additional, Henriksen, T. B., additional, Olsen, J., additional, Bosco, L., additional, Speciale, M., additional, Manno, M., additional, Amireh, N., additional, Roccheri, M. C., additional, Cittadini, E., additional, Wu, P., additional, Lee, Y. M., additional, Chen, H. W., additional, Tzeng, C. R., additional, Llacer, J., additional, Ten, J., additional, Lledo, B., additional, Rodriguez-Arnedo, A., additional, Morales, R., additional, Bernabeu, R., additional, Garcia-Peiro, A., additional, Martinez-Heredia, J., additional, Oliver-Bonet, M., additional, Ribas, J., additional, Abad, C., additional, Amengual, M. J., additional, Navarro, J., additional, Benet, J., additional, Moutou, C., additional, Gardes, N., additional, Nicod, J. C., additional, Becker, N., additional, Bailly, M. P., additional, Galland, I., additional, Pirello, O., additional, Rongieres, C., additional, Wittemer, C., additional, Viville, S., additional, Elmahaishi, W., additional, Smith, B., additional, Doshi, A., additional, Serhal, P., additional, Harper, J. C., additional, Rennemeier, C., additional, Kammerer, U., additional, Dietl, J., additional, Staib, P., additional, Elgmati, K., additional, Nomikos, M., additional, Theodoridou, M., additional, Calver, B., additional, Swann, K., additional, Lai, F. A., additional, Georgiou, I., additional, Lazaros, L., additional, Xita, N., additional, Kaponis, A., additional, Plachouras, N., additional, Hatzi, E., additional, Zikopoulos, K., additional, Ferfouri, F., additional, Clement, P., additional, Molina Gomes, D., additional, Albert, M., additional, Bailly, M., additional, Wainer, R., additional, Selva, J., additional, Vialard, F., additional, Takisawa, T., additional, Usui, K., additional, Kyoya, T., additional, Shibuya, Y., additional, Hattori, H., additional, Sato, Y., additional, Ota, M., additional, Kyono, K., additional, Chiu, P. C., additional, Lam, K. K., additional, Lee, C. L., additional, Chung, M. K., additional, Huang, V. W., additional, O, W. S., additional, Tang, F., additional, Ho, P. C., additional, Yeung, W. S., additional, Kim, C. H., additional, Lee, J. Y., additional, Kim, S. H., additional, Suh, C. S., additional, Shin, Y. K., additional, Kang, Y. J., additional, Jung, J. H., additional, Cha, C. Y., additional, Hwang, E. S., additional, Mukaida, T., additional, Nagaba, M., additional, Takahashi, K., additional, Elkaffash, D., additional, Sedrak, M., additional, Huhtaniemi, I., additional, Abdel-Al, T., additional, Younan, D., additional, Cassuto, N. G., additional, Bouret, D., additional, Hammoud, I., additional, Barak, Y., additional, Seshadri, S., additional, Bates, M., additional, Vince, G., additional, Jones, D. I., additional, Ben Khalifa, M., additional, Montjean, D., additional, Cohen-Bacrie, P., additional, Aubriot, F. X., additional, Cohen, M., additional, Boudjema, E., additional, Magli, M. C., additional, Crippa, A., additional, Baccetti, B., additional, Ferraretti, A. P., additional, Gianaroli, L., additional, Singer, T., additional, Neri, Q. V., additional, Hu, J. C., additional, Maggiulli, R., additional, Kollman, Z., additional, Rauch, E., additional, Schlegel, P. N., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Zorn, B., additional, Skrbinc, B., additional, Matos, E., additional, Golob, B., additional, Pfeifer, M., additional, Osredkar, J., additional, Sabanegh, E., additional, Sharma, R. K., additional, Thiyagarajan, A., additional, Agarwal, A., additional, Robin, G., additional, Boitrelle, F., additional, Marcelli, F., additional, Marchetti, C., additional, Mitchell, V., additional, Dewailly, D., additional, Rigot, J. M., additional, Rives, N., additional, Perdrix, A., additional, Travers, A., additional, Milazzo, J. P., additional, Mousset-Simeon, N., additional, Mace, B., additional, Jakab, A., additional, Molnar, Z., additional, Benyo, M., additional, Levai, I., additional, Kassai, Z., additional, Ihan, A., additional, Kopitar, A., additional, Kolbezen, M., additional, Vaamonde, D., additional, Da Silva-Grigoletto, M. E., additional, Garcia-Manso, J. M., additional, Vaamonde-Lemos, R., additional, Oehninger, S. C., additional, Walis, G., additional, Monahan, D., additional, Ermolovich, E., additional, Fadlon, E., additional, Abu Elhija, A., additional, Abu Elhija, M., additional, Lunenfeld, E., additional, Huleihel, M., additional, Costantini-Ferrando, M., additional, Hu, J. C. Y., additional, Alvarez, J. G., additional, Velilla, E., additional, Lopez-Teijon, M., additional, Lopez-Fernandez, C., additional, Tempest, H. G., additional, Sun, F., additional, Ko, E., additional, Turek, P., additional, Martin, R. H., additional, Zomeno-Abellan, M. T., additional, Ramirez, A., additional, Gutierrez-Adan, A., additional, Martinez, J. C., additional, Landeras, J., additional, Ballesta, J., additional, Aviles, M., additional, Ganaiem, M., additional, Binder, S., additional, Meinhardt, A., additional, Sousa, L., additional, Grangeia, A., additional, Carvalho, F., additional, Sousa, M., additional, Barros, A., additional, Sifer, C., additional, Sermondade, N., additional, Hafhouf, E., additional, Poncelet, C., additional, Benzacken, B., additional, Levy, R., additional, Wolf, J. P., additional, Crisol, L., additional, Aspichueta, F., additional, Hernandez, M. L., additional, Exposito, A., additional, Matorras, R., additional, Ruiz-Larrea, M. B., additional, Ruiz-Sanz, J. I., additional, Jallad, S., additional, Atig, F., additional, Ben Amor, H., additional, Saad, A. L. I., additional, Kerkeni, A., additional, Ajina, M., additional, Othmane, A. L. I., additional, Koscinski, I., additional, Ladureau, L., additional, Scarselli, F., additional, Casciani, V., additional, Lobascio, M., additional, Minasi, M. G., additional, Rubino, P., additional, Colasante, A., additional, Arizzi, L., additional, Litwicka, K., additional, Iammarrone, E., additional, Ferrero, S., additional, Mencacci, C., additional, Franco, G., additional, Zavaglia, D., additional, Nagy, Z. P., additional, Greco, E., additional, Ohgi, S., additional, Takahashi, M., additional, Kishi, C., additional, Suga, K., additional, Yanaihara, A., additional, Chamley, L. W., additional, Wagner, A., additional, and Shelling, A. N., additional
- Published
- 2010
- Full Text
- View/download PDF
10. Twin pregnancy after elongated spermatid injection (ELSI)
- Author
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Valcarcel, A., primary, Tiveron, M., additional, Buzzi, J., additional, Oses, R., additional, Medel, P., additional, and Young, E.T., additional
- Published
- 2008
- Full Text
- View/download PDF
11. Poor sperm quality negatively impacts embryo development and reproductive outcome in donor oocyte assisted reproductive technology cycles
- Author
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Buzzi, J., primary, Vilela, M., additional, Valcarcel, A., additional, Oses, R., additional, Young, E.T., additional, and Kenny, K., additional
- Published
- 2008
- Full Text
- View/download PDF
12. Are testicular sperm in epididymal necrospermia (EN) normal?
- Author
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Oses, R., primary, Valcarcel, A., additional, Tiveron, M., additional, Lombardi, E.P., additional, Medel, P., additional, and Chemes, H., additional
- Published
- 2008
- Full Text
- View/download PDF
13. Ultrastructural Pathology of the Sperm Flagellum
- Author
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Chemes, H. E., primary, Olmedo, S. Brugo, additional, Carrere, C., additional, Oses, R., additional, Carizza, C., additional, Leisner, M., additional, and Blaquier, J., additional
- Published
- 1999
- Full Text
- View/download PDF
14. Ultrastructural pathology of the sperm flagellum: association between flagellar pathology and fertility prognosis in severely asthenozoospermic men
- Author
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Chemes, H. E., primary, Olmedo, S. B., additional, Carrere, C., additional, Oses, R., additional, Carizza, C., additional, Leisner, M., additional, and Blaquier, J., additional
- Published
- 1998
- Full Text
- View/download PDF
15. Prognostic value of sperm morphology in intrauterine insemination
- Author
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De Caro, R.V., Osés, R., Sícaro, L.V., Valcarcel, A., and Lombardi, E.P.
- Published
- 2010
- Full Text
- View/download PDF
16. Synthesis and Characterization of Mixed-Phosphine Osmium Polyhydrides: Hydrogen Delocalization in [OsH<INF>5</INF>P<INF>3</INF>]<SUP>+</SUP> Systems
- Author
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Esteruelas, M. A., Lledos, A., Martin, M., Maseras, F., Oses, R., Ruiz, N., and Tomas, J.
- Abstract
The hexahydrido complex OsH
6 (PiPr3 )2 (1 ) reacts with PHPh2 to give molecular hydrogen and the tetrahydride OsH4 (PHPh2 )(PiPr3 )2 (2 ). However, the formation of OsH2 (PHPh2 )2 (PiPr3 )2 (3 ) as a consequence of the substitution of a second hydrogen molecule from2 by PHPh2 does not occur. The treatment of2 with 1.0 equiv of PHPh2 in toluene at 80 °C leads after 3 days to OsH2 (PHPh2 )3 (PiPr3 ) (4 ). The preparation of3 requires the previous acidolysis of2 with HBF4 , which gives [OsH5 (PHPh2 )(PiPr3 )2 ]BF4 (5 ). In contrast to2 , the addition of PHPh2 to5 affords [OsH3 (PHPh2 )2 (PiPr3 )2 ]BF4 (6 ) and molecular hydrogen. Deprotonation of6 with Et3 N yields3 . The skeleton of the cation of5 has been determined by X-ray diffraction. The configuration is consistent with a Y-shaped OsP3 disposition with the osmium atom in the common vertex. Complex5 also reacts with methanol and water to give [OsH5 {P(OMe)Ph2 }(PiPr3 )2 ]BF4 (7 ) and [OsH5 {P(OH)Ph2 }(PiPr3 )2 ]BF4 (8 ), respectively. The addition of Et3 N to7 affords OsH4 {P(OMe)Ph2 }(PiPr3 )2 (9 ). A theoretical study on the OsH5 (PH3 )3 + model complex reveals that although a static description is fully consistent with a classical pentahydride assignment, the formation of a dihydrogen is a very low energy costing process, on both thermodynamic and kinetic grounds. Thus, these polyhydride systems might be better described as possessing delocalized hydrogen atoms. A further QM/MM IMOMM study on the actual OsH5 (PR3 )+ systems indicates that the inclusion of bulky phosphine substituents plays a role against the stability of dihydrogen forms, because of the higher steric congestion of lower coordination number complexes arising from repulsions between bulky phosphines. Although IMOMM calculations improve significantly the agreement with experimental structures, they do not change the validity of the aforementioned statement concerning delocalization.- Published
- 2001
17. ENVIRONMENTAL CHARACTERIZATION AND DEVELOPMENT OF PLANETARY SCIENCE IN THE PUNA DE ATACAMA.
- Author
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Tavernier, A., Garcia, A., Ulloa, C., Oses, R., Bonnail, E., Santibañez, T., McGrath, K., Mohan, C., Reilly, H., Brady, G., and Foing, B.
- Subjects
PLANETARY science ,LUNAR exploration ,SCIENTIFIC expeditions ,EXTREME environments - Published
- 2021
18. A shooting simulator from boats
- Author
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Figueroa, P., Carlos F. Rodriguez, Hernandez, J. T., Blanco, J. C., Oses, R., and Ballesteros, L.
19. Fertility in hair sheep inseminated with freeze spermatozoa rediluited with seminal plasma,Fertilidad en ovejas de pelo inseminadas con semen congelado rediluido con plasma seminal
- Author
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Domínguez Rebolledo, A., Navarrete Sierra, L., ALVAR ALONZO CRUZ TAMAYO, Aguiar Loria, A., Erosa Denis, S., Bolio Oses, R., González Parra, E., Paredes Monsreal, L., and Ramón Ugalde, J.
20. Correlation between testicular sperm extraction (TESE) and biopsy results performed for intracytoplasmic sperm injection (ICSI) cycles.
- Author
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Oses, R. J., Bisioli, C., Tiveron, M., Medel, P., Quintana, R., and Marconi, G.
- Published
- 2001
- Full Text
- View/download PDF
21. Quinoa biodiversity and sustainability for food security under climate change. A review
- Author
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Rómulo Oses, Fabiana Antognoni, Marco A. Molina-Montenegro, Ian S. Acuña-Rodríguez, Didier Bazile, Milton Pinto, Enrique A. Martinez-Mosqueira, Karina B. Ruiz, Stefania Biondi, Alipio Canahua-Murillo, Andrés Zurita-Silva, Amadou Coulibaly, Sven-Erik Jacobsen, RUIZ K. B., BIONDI S., OSES R., ACUÑA-RODRÍGUEZ I. S., ANTOGNONI F., MARTINEZ-MOSQUEIRA E.A., COULIBALY A., CANAHUA-MURILLO A., PINTO M., ZURITA-SILVA A., BAZILE D., JACOBSEN S.-E., and MOLINA-MONTENEGRO M.A.
- Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,PLANT ABIOTIC STRESS ,QUINOA ,Biodiversity ,Stress tolerance ,Ecosystem services ,F30 - Génétique et amélioration des plantes ,E14 - Économie et politique du développement ,F01 - Culture des plantes ,Tolérance au sel ,Chenopodium quinoa ,2. Zero hunger ,[SDV.EE]Life Sciences [q-bio]/Ecology, environment ,education.field_of_study ,Food security ,Ecology ,Agroforestry ,FOOD SECURITY ,food and beverages ,Geography ,sécurité alimentaire ,Sustainability ,Agroécosystème ,Diversification ,Environmental Engineering ,P40 - Météorologie et climatologie ,F60 - Physiologie et biochimie végétale ,Population ,Petite exploitation agricole ,Variation génétique ,education ,Agroecology ,Changement climatique ,business.industry ,E80 - Économie familiale et artisanale ,15. Life on land ,Valeur nutritive ,Agrobiodiversity ,Résistance à la sécheresse ,13. Climate action ,Agriculture ,Développement durable ,Agricultural biodiversity ,business ,Agronomy and Crop Science ,Andean farmers ,Food quality - Abstract
International audience; Climate change is rapidly degrading the conditions of crop production. For instance, increasing salinization and aridity is forecasted to increase in most parts of the world. As a consequence, new stress-tolerant species and genotypes must be identified and used for future agriculture. Stress-tolerant species exist but are actually underutilized and neglected. Many stress-tolerant species are indeed traditional crops that are only cultivated by farmers at a local scale. Those species have a high biodiversity value. Besides, the human population will probably reach nine billion within coming decades. To keep pace with population growth, food production must increase dramatically despite the limited availability of cultivable land and water. Here, we review the benefits of quinoa, Chenopodium quinoa Willd., a seed crop that has endured the harsh bioclimatic conditions of the Andes since ancient times. Although the crop is still mainly produced in Bolivia and Peru, agronomic trials and cultivation are spreading to many other countries. Quinoa maintains productivity on rather poor soils and under conditions of water shortage and high salinity. Moreover, quinoa seeds are an exceptionally nutritious food source, owing to their high protein content with all essential amino acids, lack of gluten, and high content of several minerals, e.g., Ca, Mg, Fe, and health-promoting compounds such as flavonoids. Quinoa has a vast genetic diversity resulting from its fragmented and localized production over the centuries in the Andean region, from Ecuador to southern Chile, and from sea level to the altiplano. Quinoa can be adapted to diverse agroecological conditions worldwide. Year 2013 has therefore been declared the International Year of Quinoa by the United Nations Food and Agriculture Organization. Here, we review the main characteristics of quinoa, its origin and genetic diversity, its exceptional tolerance to drought and salinity, its nutritional properties, the reasons why this crop can offer several ecosystem services, and the role of Andean farmers in preserving its agrobiodiversity. Finally, we propose a schematic model integrating the fundamental factors that should determine the future utilization of quinoa, in terms of food security, biodiversity conservation, and cultural identity.
- Published
- 2014
22. Complete genome sequence of Cellulomonas sp. strain ATA003.
- Author
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Bartholomäus A, Mitzscherling J, Lipus D, Wagner D, Lavin P, Contreras R, and Oses R
- Abstract
The Gram-positive, rod-shaped endophytic bacterium Cellulomonas sp. strain ATA003 was isolated from the endemic cactus Maihueniopsis domeykoensis seeds collected in the Coastal Atacama Desert, Chile. Here, we present a circular genome with a size of 4,084,881 bp and a GC content of 73.8% obtained by Nanopore sequencing., Competing Interests: The authors declare no conflict of interest.
- Published
- 2024
- Full Text
- View/download PDF
23. Microbial impact on initial soil formation in arid and semiarid environments under simulated climate change.
- Author
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Rodríguez V, Bartholomäus A, Witzgall K, Riveras-Muñoz N, Oses R, Liebner S, Kallmeyer J, Rach O, Mueller CW, Seguel O, Scholten T, and Wagner D
- Abstract
The microbiota is attributed to be important for initial soil formation under extreme climate conditions, but experimental evidence for its relevance is scarce. To fill this gap, we investigated the impact of in situ microbial communities and their interrelationship with biocrust and plants compared to abiotic controls on soil formation in initial arid and semiarid soils. Additionally, we assessed the response of bacterial communities to climate change. Topsoil and subsoil samples from arid and semiarid sites in the Chilean Coastal Cordillera were incubated for 16 weeks under diurnal temperature and moisture variations to simulate humid climate conditions as part of a climate change scenario. Our findings indicate that microorganism-plant interaction intensified aggregate formation and stabilized soil structure, facilitating initial soil formation. Interestingly, microorganisms alone or in conjunction with biocrust showed no discernible patterns compared to abiotic controls, potentially due to water-masking effects. Arid soils displayed reduced bacterial diversity and developed a new community structure dominated by Proteobacteria , Actinobacteriota , and Planctomycetota , while semiarid soils maintained a consistently dominant community of Acidobacteriota and Proteobacteria . This highlighted a sensitive and specialized bacterial community in arid soils, while semiarid soils exhibited a more complex and stable community. We conclude that microorganism-plant interaction has measurable impacts on initial soil formation in arid and semiarid regions on short time scales under climate change. Additionally, we propose that soil and climate legacies are decisive for the present soil microbial community structure and interactions, future soil development, and microbial responses., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Rodríguez, Bartholomäus, Witzgall, Riveras-Muñoz, Oses, Liebner, Kallmeyer, Rach, Mueller, Seguel, Scholten and Wagner.)
- Published
- 2024
- Full Text
- View/download PDF
24. An improved method for intracellular DNA (iDNA) recovery from terrestrial environments.
- Author
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Medina Caro D, Horstmann L, Ganzert L, Oses R, Friedl T, and Wagner D
- Subjects
- Edetic Acid, Phosphates, Ecosystem, DNA genetics
- Abstract
The simultaneous extraction of intracellular DNA (iDNA) and extracellular DNA (eDNA) can help to separate the living in situ community (represented by iDNA) from background DNA that originated both from past communities and from allochthonous sources. As iDNA and eDNA extraction protocols require separating cells from the sample matrix, their DNA yields are generally lower than direct methods that lyse the cells within the sample matrix. We, therefore, tested different buffers with and without adding a detergent mix (DM) in the extraction protocol to improve the recovery of iDNA from surface and subsurface samples that covered a variety of terrestrial environments. The combination of a highly concentrated sodium phosphate buffer plus DM significantly improved iDNA recovery for almost all tested samples. Additionally, the combination of sodium phosphate and EDTA improved iDNA recovery in most of the samples and even allowed the successful extraction of iDNA from extremely low-biomass iron-bearing rock samples taken from the deep biosphere. Based on our results, we recommend using a protocol with sodium phosphate in combination with either a DM (NaP 300 mM + DM) or EDTA (NaP + EDTA 300 mM). Furthermore, for studies that rely on the eDNA pool, we recommend using buffers solely based on sodium phosphate because the addition of EDTA or a DM resulted in a decrease in eDNA for most of the tested samples. These improvements can help reduce community bias in environmental studies and contribute to better characterizations of both modern and past ecosystems., (© 2023 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
25. Impact of Climate and Slope Aspects on the Composition of Soil Bacterial Communities Involved in Pedogenetic Processes along the Chilean Coastal Cordillera.
- Author
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Rodriguez V, Moskwa LM, Oses R, Kühn P, Riveras-Muñoz N, Seguel O, Scholten T, and Wagner D
- Abstract
Soil bacteria play a fundamental role in pedogenesis. However, knowledge about both the impact of climate and slope aspects on microbial communities and the consequences of these items in pedogenesis is lacking. Therefore, soil-bacterial communities from four sites and two different aspects along the climate gradient of the Chilean Coastal Cordillera were investigated. Using a combination of microbiological and physicochemical methods, soils that developed in arid, semi-arid, mediterranean, and humid climates were analyzed. Proteobacteria , Acidobacteria , Chloroflexi , Verrucomicrobia , and Planctomycetes were found to increase in abundance from arid to humid climates, while Actinobacteria and Gemmatimonadetes decreased along the transect. Bacterial-community structure varied with climate and aspect and was influenced by pH, bulk density, plant-available phosphorus, clay, and total organic-matter content. Higher bacterial specialization was found in arid and humid climates and on the south-facing slope and was likely promoted by stable microclimatic conditions. The presence of specialists was associated with ecosystem-functional traits, which shifted from pioneers that accumulated organic matter in arid climates to organic decomposers in humid climates. These findings provide new perspectives on how climate and slope aspects influence the composition and functional capabilities of bacteria, with most of these capabilities being involved in pedogenetic processes.
- Published
- 2022
- Full Text
- View/download PDF
26. Draft Genome Sequence of Antarctic Psychrotroph Streptomyces fildesensis Strain INACH3013, Isolated from King George Island Soil.
- Author
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Lavin P, Henríquez-Castillo C, Yong ST, Valenzuela-Heredia D, Oses R, Frez K, Borba MP, Purcarea C, and Wong CMVL
- Abstract
The draft genome sequence of Streptomyces fildesensis strain INACH3013, a psychrotrophic bacterium isolated from Northwest Antarctic soil, was reported. The genome sequence totaling 9,306,785 bp resulted from 122 contigs characterized by a GC content of 70.55%., (Copyright © 2021 Lavin et al.)
- Published
- 2021
- Full Text
- View/download PDF
27. Root-endophytes improve the ecophysiological performance and production of an agricultural species under drought condition.
- Author
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Molina-Montenegro MA, Oses R, Torres-Díaz C, Atala C, Zurita-Silva A, and Ruiz-Lara S
- Abstract
Throughout many regions of the world, climate change has limited the availability of water for irrigating crops. Indeed, current models of climate change predict that arid and semi-arid zones will be places where precipitation will drastically decrease. In this context, plant root-associated fungi appear as a new strategy to improve ecophysiological performance and yield of crops under abiotic stress. Thus, use of fungal endophytes from ecosystems currently subjected to severe drought conditions could improve the ecophysiological performance and quantum yield of crops exposed to drought. In this study, we evaluated how the inoculation of fungal endophytes isolated from Antarctic plants can improve the net photosynthesis, water use efficiency and production of fresh biomass in a lettuce cultivar, grown under different water availability regimes. In addition, we assessed if the presence of biochemical mechanisms and gene expression related with environmental tolerance are improved in presence of fungal endophytes. Overall, those individuals with presence of endophytes showed higher net photosynthesis and maintained higher water use efficiency in drought conditions, which was correlated with greater fresh and dry biomass production as well as greater root system development. In addition, presence of fungal endophytes was correlated with a higher proline concentration, lower peroxidation of lipids and up-/down-regulation of ion homeostasis. Our results suggest that presence of fungal endophytes could minimize the negative effect of drought by improving drought tolerance through biochemical mechanisms and improving nutritional status. Thus, root-endophytes might be a successful biotechnological tool to maintain high levels of ecophysiological performance and productivity in zones under drought., (© The Authors 2016. Published by Oxford University Press on behalf of the Annals of Botany Company.)
- Published
- 2016
- Full Text
- View/download PDF
28. Biological Interactions and Simulated Climate Change Modulates the Ecophysiological Performance of Colobanthus quitensis in the Antarctic Ecosystem.
- Author
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Torres-Díaz C, Gallardo-Cerda J, Lavin P, Oses R, Carrasco-Urra F, Atala C, Acuña-Rodríguez IS, Convey P, and Molina-Montenegro MA
- Subjects
- Antarctic Regions, Biomass, Caryophyllaceae chemistry, Caryophyllaceae microbiology, Climate Change, Ecosystem, Temperature, Caryophyllaceae physiology, Endophytes physiology, Phytochemicals analysis
- Abstract
Most climate and environmental change models predict significant increases in temperature and precipitation by the end of the 21st Century, for which the current functional output of certain symbioses may also be altered. In this context we address the following questions: 1) How the expected changes in abiotic factors (temperature, and water) differentially affect the ecophysiological performance of the plant Colobanthus quitensis? and 2) Will this environmental change indirectly affect C. quitensis photochemical performance and biomass accumulation by modifying its association with fungal endophytes? Plants of C. quitensis from King George Island in the South Shetland archipelago (62°09' S), and Lagotellerie Island in the Antarctic Peninsula (65°53' S) were put under simulated abiotic conditions in growth chambers following predictive models of global climate change (GCC). The indirect effect of GCC on the interaction between C. quitensis and fungal endophytes was assessed in a field experiment carried out in the Antarctica, in which we eliminated endophytes under contemporary conditions and applied experimental watering to simulate increased precipitation input. We measured four proxies of plant performance. First, we found that warming (+W) significantly increased plant performance, however its effect tended to be less than watering (+W) and combined warming and watering (+T°+W). Second, the presence of fungal endophytes improved plant performance, and its effect was significantly decreased under experimental watering. Our results indicate that both biotic and abiotic factors affect ecophysiological performance, and the directions of these influences will change with climate change. Our findings provide valuable information that will help to predict future population spread and evolution through using ecological niche models under different climatic scenarios., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2016
- Full Text
- View/download PDF
29. Is physiological performance a good predictor for fitness? Insights from an invasive plant species.
- Author
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Molina-Montenegro MA, Salgado-Luarte C, Oses R, and Torres-Díaz C
- Subjects
- Environment, Phenotype, Seeds, Taraxacum physiology, Genetic Fitness, Introduced Species, Plant Physiological Phenomena
- Abstract
Is physiological performance a suitable proxy of fitness in plants? Although, several studies have been conducted to measure some fitness-related traits and physiological performance, direct assessments are seldom found in the literature. Here, we assessed the physiology-fitness relationship using second-generation individuals of the invasive plant species Taraxacum officinale from 17 localities distributed in five continents. Specifically, we tested if i) the maximum quantum yield is a good predictor for seed-output ii) whether this physiology-fitness relationship can be modified by environmental heterogeneity, and iii) if this relationship has an adaptive consequence for T. officinale individuals from different localities. Overall, we found a significant positive relationship between the maximum quantum yield and fitness for all localities evaluated, but this relationship decreased in T. officinale individuals from localities with greater environmental heterogeneity. Finally, we found that those individuals from localities where environmental conditions are highly seasonal performed better under heterogeneous environmental conditions. Contrarily, under homogeneous controlled conditions, those individuals from localities with low environmental seasonality performed much better. In conclusion, our results suggest that the maximum quantum yield seem to be good predictors for plant fitness. We suggest that rapid measurements, such as those obtained from the maximum quantum yield, could provide a straightforward proxy of individual's fitness in changing environments.
- Published
- 2013
- Full Text
- View/download PDF
30. Hypogonadal impotence treated by transdermal testosterone.
- Author
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McClure RD, Oses R, and Ernest ML
- Subjects
- Administration, Cutaneous, Adult, Clinical Protocols, Drug Administration Schedule, Erectile Dysfunction blood, Erectile Dysfunction etiology, Humans, Hypogonadism blood, Hypogonadism complications, Male, Middle Aged, Erectile Dysfunction drug therapy, Testosterone therapeutic use
- Abstract
The transdermal therapeutic system for testosterone (TTS-T) (ALZA Corp.), applied to the scrotal skin for twenty-two hours daily, was tested for twelve weeks on 4 men with hypogonadal impotence; 2 of these men wore TTS-T for as long as twenty-six months. The 40 cm2 system delivered a daily dose of 2.8 +/- 0.16 (S.E.) mg (nominal dose 2.4 mg) and the 60 cm2 system delivered a dose of 3.99 +/- 0.24 mg (nominal dose 3.6 mg). Both systems promptly increased serum testosterone and dihydrotestosterone (DHT) to physiologic levels, restoring normal erectile activity with an increased frequency of ejaculation and a positive effect on both mood and energy. There were no changes in serum sex binding globulin and estradiol, prostate or breast size, hematologic or liver function measures, or urinary flow and frequency. There were no significant changes in serum cholesterol or low-density lipoproteins, but high-density lipoproteins tended to decline slightly. There were no dermatologic problems associated with the system. The tenfold increase in DHT over baseline levels was attributed to 5-alpha reduction of testosterone in the scrotal skin. The TTS-T is convenient, reliable, and mimics normal physiologic testosterone secretion and levels more closely than conventional methods of testosterone replacement.
- Published
- 1991
- Full Text
- View/download PDF
31. Does sexual intercourse improve pregnancy rates in gamete intrafallopian transfer?
- Author
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Marconi G, Auge L, Oses R, Quintana R, Raffo F, and Young E
- Subjects
- Adult, Chorionic Gonadotropin therapeutic use, Clinical Trials as Topic, Female, Humans, Oocytes cytology, Ovarian Follicle cytology, Ovarian Follicle physiology, Pregnancy, Random Allocation, Coitus, Gamete Intrafallopian Transfer, Pregnancy Outcome
- Published
- 1989
- Full Text
- View/download PDF
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