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706 results on '"Osada, Takuya"'

3. Effects of Aerobic Cycling Training on O2 Dynamics in Several Leg Muscles in Early Post-myocardial Infarction

Author

Takagi, Shun, Kime, Ryotaro, Murase, Norio, Niwayama, Masatsugu, Osada, Takuya, Katsumura, Toshihito, COHEN, IRUN R., Editorial Board Member, LAJTHA, ABEL, Editorial Board Member, LAMBRIS, JOHN D., Editorial Board Member, PAOLETTI, RODOLFO, Editorial Board Member, REZAEI, NIMA, Editorial Board Member, Thews, Oliver, editor, LaManna, Joseph C., editor, and Harrison, David K., editor

Published
2018
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4. The use of histotripsy as intratumoral immunotherapy beyond tissue ablation—the rationale for exploring the immune effects of histotripsy

Author

Osada, Takuya, primary, Jiang, Xiaoning, additional, Zhao, Yuhang, additional, Chen, Mengyue, additional, Kreager, Benjamin C., additional, Wu, Huaiyu, additional, Kim, Howuk, additional, Ren, Jun, additional, Snyder, Joshua, additional, Zhong, Pei, additional, Morse, Michael A., additional, and Lyerly, H. Kim, additional

Published
2023
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5. A novel diagnostic examination for dropped head syndrome (DHS) (Prone position cervical extension test; DHS test)

Author

Sano, Hiroki, primary, Endo, Kenji, additional, Sawaji, Yasunobu, additional, Aihara, Takahito, additional, Suzuki, Hidekazu, additional, Yamauchi, Tomoyasu, additional, Ishiyama, Masahiro, additional, Osada, Takuya, additional, Ueno, Ryuuichi, additional, Masaoka, Toshinori, additional, Nishimura, Hirosuke, additional, and Yamamoto, Kengo, additional

Published
2023
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9. Evidence for phenotypic plasticity in aggressive triple-negative breast cancer: human biology is recapitulated by a novel model system.

Author

D'Amato, Nicholas C, Ostrander, Julie H, Bowie, Michelle L, Sistrunk, Christopher, Borowsky, Alexander, Cardiff, Robert D, Bell, Katie, Young, Lawrence JT, Simin, Karl, Bachelder, Robin E, Delrow, Jeff, Dawson, Alyssa, Yee, Lisa D, Mrózek, Krzysztof, Clay, Timothy M, Osada, Takuya, and Seewaldt, Victoria L

Subjects
Cell Line, Tumor, Bone Marrow, Animals, Humans, Mice, Breast Neoplasms, Neoplasm Metastasis, Culture Media, Serum-Free, Prognosis, Karyotyping, Gene Expression Profiling, Neoplasm Transplantation, Cytogenetics, Gene Expression Regulation, Neoplastic, Phenotype, Exons, Adult, Female, Epithelial-Mesenchymal Transition, Cell Line, Tumor, Culture Media, Serum-Free, Gene Expression Regulation, Neoplastic, General Science & Technology
Abstract

Breast cancers with a basal-like gene signature are primarily triple-negative, frequently metastatic, and carry a poor prognosis. Basal-like breast cancers are enriched for markers of breast cancer stem cells as well as markers of epithelial-mesenchymal transition (EMT). While EMT is generally thought to be important in the process of metastasis, in vivo evidence of EMT in human disease remains rare. Here we report a novel model of human triple-negative breast cancer, the DKAT cell line, which was isolated from an aggressive, treatment-resistant triple-negative breast cancer that demonstrated morphological and biochemical evidence suggestive of phenotypic plasticity in the patient. The DKAT cell line displays a basal-like phenotype in vitro when cultured in serum-free media, and undergoes phenotypic changes consistent with EMT/MET in response to serum-containing media, a unique property among the breast cancer cell lines we tested. This EMT is marked by increased expression of the transcription factor Zeb1, and Zeb1 is required for the enhanced migratory ability of DKAT cells in the mesenchymal state. DKAT cells also express progenitor-cell markers, and single DKAT cells are able to generate tumorspheres containing both epithelial and mesenchymal cell types. In vivo, as few as ten DKAT cells are capable of forming xenograft tumors which display a range of epithelial and mesenchymal phenotypes. The DKAT model provides a novel model to study the molecular mechanisms regulating phenotypic plasticity and the aggressive biology of triple-negative breast cancers.

Published
2012

10. Evidence for Phenotypic Plasticity in Aggressive Triple-Negative Breast Cancer: Human Biology Is Recapitulated by a Novel Model System

Author

D’Amato, Nicholas C, Ostrander, Julie H, Bowie, Michelle L, Sistrunk, Christopher, Borowsky, Alexander, Cardiff, Robert D, Bell, Katie, Young, Lawrence JT, Simin, Karl, Bachelder, Robin E, Delrow, Jeff, Dawson, Alyssa, Yee, Lisa D, Mrózek, Krzysztof, Clay, Timothy M, Osada, Takuya, and Seewaldt, Victoria L

Subjects
Biochemistry and Cell Biology, Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Biological Sciences, Breast Cancer, Biotechnology, Stem Cell Research, Genetics, Stem Cell Research - Nonembryonic - Non-Human, Stem Cell Research - Nonembryonic - Human, Cancer, 2.1 Biological and endogenous factors, Aetiology, Adult, Animals, Bone Marrow, Breast Neoplasms, Cell Line, Tumor, Culture Media, Serum-Free, Cytogenetics, Epithelial-Mesenchymal Transition, Exons, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Karyotyping, Mice, Neoplasm Metastasis, Neoplasm Transplantation, Phenotype, Prognosis, General Science & Technology
Abstract

Breast cancers with a basal-like gene signature are primarily triple-negative, frequently metastatic, and carry a poor prognosis. Basal-like breast cancers are enriched for markers of breast cancer stem cells as well as markers of epithelial-mesenchymal transition (EMT). While EMT is generally thought to be important in the process of metastasis, in vivo evidence of EMT in human disease remains rare. Here we report a novel model of human triple-negative breast cancer, the DKAT cell line, which was isolated from an aggressive, treatment-resistant triple-negative breast cancer that demonstrated morphological and biochemical evidence suggestive of phenotypic plasticity in the patient. The DKAT cell line displays a basal-like phenotype in vitro when cultured in serum-free media, and undergoes phenotypic changes consistent with EMT/MET in response to serum-containing media, a unique property among the breast cancer cell lines we tested. This EMT is marked by increased expression of the transcription factor Zeb1, and Zeb1 is required for the enhanced migratory ability of DKAT cells in the mesenchymal state. DKAT cells also express progenitor-cell markers, and single DKAT cells are able to generate tumorspheres containing both epithelial and mesenchymal cell types. In vivo, as few as ten DKAT cells are capable of forming xenograft tumors which display a range of epithelial and mesenchymal phenotypes. The DKAT model provides a novel model to study the molecular mechanisms regulating phenotypic plasticity and the aggressive biology of triple-negative breast cancers.

Published
2012

12. Muscle Oxygen Dynamics During Cycling Exercise in Angina Pectoris Patients

Author

Takagi, Shun, Murase, Norio, Kime, Ryotaro, Niwayama, Masatsugu, Osada, Takuya, Katsumura, Toshihito, COHEN, IRUN R., Series editor, LAJTHA, ABEL, Series editor, LAMBRIS, JOHN D., Series editor, PAOLETTI, RODOLFO, Series editor, Luo, Qingming, editor, Li, Lin Z., editor, Harrison, David K., editor, Shi, Hua, editor, and Bruley, Duane F., editor

Published
2016
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13. Muscle Deoxygenation and Its Heterogeneity Changes After Endurance Training

Author

Kime, Ryotaro, Niwayama, Masatsugu, Kaneko, Yasuhisa, Takagi, Shun, Fuse, Sayuri, Osada, Takuya, Murase, Norio, Katsumura, Toshihito, COHEN, IRUN R., Series editor, LAJTHA, ABEL, Series editor, LAMBRIS, JOHN D., Series editor, PAOLETTI, RODOLFO, Series editor, Luo, Qingming, editor, Li, Lin Z., editor, Harrison, David K., editor, Shi, Hua, editor, and Bruley, Duane F., editor

Published
2016
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18. Data from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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19. Supplementary Table 1 from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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20. Supplementary Figure 1 from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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21. Supplementary Table 3 from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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22. Supplementary Materials and Methods from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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23. Supplementary Figure Legends 1-2 from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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24. Supplementary Figure 2 from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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25. Supplementary Table 2 from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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26. Supplementary Table 4 from Characterization of an Oxaliplatin Sensitivity Predictor in a Preclinical Murine Model of Colorectal Cancer

Author

Kim, Mickey K., primary, Osada, Takuya, primary, Barry, William T., primary, Yang, Xiao Yi, primary, Freedman, Jennifer A., primary, Tsamis, Katherine A., primary, Datto, Michael, primary, Clary, Bryan M., primary, Clay, Timothy, primary, Morse, Michael A., primary, Febbo, Philip G., primary, Lyerly, H. Kim, primary, and Hsu, David S., primary

Published
2023
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27. Figure S2 from Intratumoral Plasmid IL12 Expands CD8+ T Cells and Induces a CXCR3 Gene Signature in Triple-negative Breast Tumors that Sensitizes Patients to Anti–PD-1 Therapy

Author

Telli, Melinda L., primary, Nagata, Hiroshi, primary, Wapnir, Irene, primary, Acharya, Chaitanya R., primary, Zablotsky, Kaitlin, primary, Fox, Bernard A., primary, Bifulco, Carlo B., primary, Jensen, Shawn M., primary, Ballesteros-Merino, Carmen, primary, Le, Mai Hope, primary, Pierce, Robert H., primary, Browning, Erica, primary, Hermiz, Reneta, primary, Svenson, Lauren, primary, Bannavong, Donna, primary, Jaffe, Kim, primary, Sell, Jendy, primary, Foerter, Kellie Malloy, primary, Canton, David A., primary, Twitty, Christopher G., primary, Osada, Takuya, primary, Lyerly, H. Kim, primary, and Crosby, Erika J., primary

Published
2023
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28. Supplementary Data from An Adenoviral Vaccine Encoding Full-Length Inactivated Human Her2 Exhibits Potent Immunogenicty and Enhanced Therapeutic Efficacy without Oncogenicity

Author

Hartman, Zachary C., primary, Wei, Junping, primary, Osada, Takuya, primary, Glass, Oliver, primary, Lei, Gangjun, primary, Yang, Xiao-Yi, primary, Peplinski, Sharon, primary, Kim, Dong-Wan, primary, Xia, Wenle, primary, Spector, Neil, primary, Marks, Jeffrey, primary, Barry, William, primary, Hobeika, Amy, primary, Devi, Gayathri, primary, Amalfitano, Andrea, primary, Morse, Michael A., primary, Lyerly, H. Kim, primary, and Clay, Timothy M., primary

Published
2023
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31. Figure S1 from Vaccine-Induced Memory CD8+ T Cells Provide Clinical Benefit in HER2 Expressing Breast Cancer: A Mouse to Human Translational Study

Author

Crosby, Erika J., primary, Gwin, William, primary, Blackwell, Kimberly, primary, Marcom, Paul K., primary, Chang, Serena, primary, Maecker, Holden T., primary, Broadwater, Gloria, primary, Hyslop, Terry, primary, Kim, Sungjin, primary, Rogatko, Andre, primary, Lubkov, Veronica, primary, Snyder, Joshua C., primary, Osada, Takuya, primary, Hobeika, Amy C., primary, Morse, Michael A., primary, Lyerly, H. Kim, primary, and Hartman, Zachary C., primary

Published
2023
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32. Table S4 from Intratumoral Plasmid IL12 Expands CD8+ T Cells and Induces a CXCR3 Gene Signature in Triple-negative Breast Tumors that Sensitizes Patients to Anti–PD-1 Therapy

Author

Telli, Melinda L., primary, Nagata, Hiroshi, primary, Wapnir, Irene, primary, Acharya, Chaitanya R., primary, Zablotsky, Kaitlin, primary, Fox, Bernard A., primary, Bifulco, Carlo B., primary, Jensen, Shawn M., primary, Ballesteros-Merino, Carmen, primary, Le, Mai Hope, primary, Pierce, Robert H., primary, Browning, Erica, primary, Hermiz, Reneta, primary, Svenson, Lauren, primary, Bannavong, Donna, primary, Jaffe, Kim, primary, Sell, Jendy, primary, Foerter, Kellie Malloy, primary, Canton, David A., primary, Twitty, Christopher G., primary, Osada, Takuya, primary, Lyerly, H. Kim, primary, and Crosby, Erika J., primary

Published
2023
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36. Supplementary Table 1 from Phase I Study Utilizing a Novel Antigen-Presenting Cell–Targeted Vaccine with Toll-like Receptor Stimulation to Induce Immunity to Self-antigens in Cancer Patients

Author

Morse, Michael A., primary, Chapman, Robert, primary, Powderly, John, primary, Blackwell, Kimberly, primary, Keler, Tibor, primary, Green, Jennifer, primary, Riggs, Renee, primary, He, Li-Zhen, primary, Ramakrishna, Venky, primary, Vitale, Laura, primary, Zhao, Biwei, primary, Butler, Stephen A., primary, Hobeika, Amy, primary, Osada, Takuya, primary, Davis, Thomas, primary, Clay, Timothy, primary, and Lyerly, H. Kim, primary

Published
2023
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38. Data from Phase I Study Utilizing a Novel Antigen-Presenting Cell–Targeted Vaccine with Toll-like Receptor Stimulation to Induce Immunity to Self-antigens in Cancer Patients

Author

Morse, Michael A., primary, Chapman, Robert, primary, Powderly, John, primary, Blackwell, Kimberly, primary, Keler, Tibor, primary, Green, Jennifer, primary, Riggs, Renee, primary, He, Li-Zhen, primary, Ramakrishna, Venky, primary, Vitale, Laura, primary, Zhao, Biwei, primary, Butler, Stephen A., primary, Hobeika, Amy, primary, Osada, Takuya, primary, Davis, Thomas, primary, Clay, Timothy, primary, and Lyerly, H. Kim, primary

Published
2023
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39. Data from Vaccine-Induced Memory CD8+ T Cells Provide Clinical Benefit in HER2 Expressing Breast Cancer: A Mouse to Human Translational Study

Author

Crosby, Erika J., primary, Gwin, William, primary, Blackwell, Kimberly, primary, Marcom, Paul K., primary, Chang, Serena, primary, Maecker, Holden T., primary, Broadwater, Gloria, primary, Hyslop, Terry, primary, Kim, Sungjin, primary, Rogatko, Andre, primary, Lubkov, Veronica, primary, Snyder, Joshua C., primary, Osada, Takuya, primary, Hobeika, Amy C., primary, Morse, Michael A., primary, Lyerly, H. Kim, primary, and Hartman, Zachary C., primary

Published
2023
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40. Table S2 from Vaccine-Induced Memory CD8+ T Cells Provide Clinical Benefit in HER2 Expressing Breast Cancer: A Mouse to Human Translational Study

Author

Crosby, Erika J., primary, Gwin, William, primary, Blackwell, Kimberly, primary, Marcom, Paul K., primary, Chang, Serena, primary, Maecker, Holden T., primary, Broadwater, Gloria, primary, Hyslop, Terry, primary, Kim, Sungjin, primary, Rogatko, Andre, primary, Lubkov, Veronica, primary, Snyder, Joshua C., primary, Osada, Takuya, primary, Hobeika, Amy C., primary, Morse, Michael A., primary, Lyerly, H. Kim, primary, and Hartman, Zachary C., primary

Published
2023
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41. Supplementary methods from Intratumoral Plasmid IL12 Expands CD8+ T Cells and Induces a CXCR3 Gene Signature in Triple-negative Breast Tumors that Sensitizes Patients to Anti–PD-1 Therapy

Author

Telli, Melinda L., primary, Nagata, Hiroshi, primary, Wapnir, Irene, primary, Acharya, Chaitanya R., primary, Zablotsky, Kaitlin, primary, Fox, Bernard A., primary, Bifulco, Carlo B., primary, Jensen, Shawn M., primary, Ballesteros-Merino, Carmen, primary, Le, Mai Hope, primary, Pierce, Robert H., primary, Browning, Erica, primary, Hermiz, Reneta, primary, Svenson, Lauren, primary, Bannavong, Donna, primary, Jaffe, Kim, primary, Sell, Jendy, primary, Foerter, Kellie Malloy, primary, Canton, David A., primary, Twitty, Christopher G., primary, Osada, Takuya, primary, Lyerly, H. Kim, primary, and Crosby, Erika J., primary

Published
2023
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