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Undecided students who self-identify as uncertain of their academic path represent a unique population to support from an academic advising perspective. In a sample of 257,813 students who entered 243 four-year institutions nationally, this study examined the relationship between students who selected undecided as their intended college major and individual student characteristics. Results indicate a primary predictor of undecidedness is student acknowledgment of indecisiveness about their choice of major or career; however, undecidedness also is related to interpersonal and contextual characteristics previously unexamined. Advisors should assess students' level of major and career certainty, concerns about financing college, and aspects of students' identities that were predictive of undecidedness. [ABSTRACT FROM AUTHOR]

Abstract An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology is shown to be as effective, less labour-intensive, reduces animal handling and animal numbers required for successful selection thereby contributing to two of the "three Rs" of animal experimentation, namely refinement and reduction.

Gene manipulation is an invaluable tool to investigate and understand the biology of an organism. Although this technology has been applied to both the human and rodent malarial parasites (RMP), Plasmodium berghei in particular offers a more robust system due to a higher and more efficient transformation rate. Here, we describe a comprehensive transfection and selection protocol using P. berghei including a variant negative selection protocol administering 5-fluorocytosine to the animals in drinking water. Additionally, we discuss and assess the latest advances in gene manipulation technologies developed in RMP to gain a better understanding of Plasmodium biology.