1. Genotoxic and cytotoxic assessment of two endemic Lamiaceae species from Bosnia and Herzegovina
- Author
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Sanja Ćavar Zeljković, Sanin Haverić, Maida Hadžić, Jasmina Čakar, Tamara Ćetković, Mahira Mehanović, and Anja Haverić
- Subjects
Bosnia and Herzegovina ,Lamiaceae ,food.ingredient ,biology ,Traditional medicine ,Plant Extracts ,010405 organic chemistry ,Organic Chemistry ,Plant Science ,biology.organism_classification ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,food ,Phenols ,Clinopodium alpinum ,Humans ,Cytotoxic T cell ,Dimethyl Sulfoxide ,Thymus bracteosus ,Orontium ,DNA Damage - Abstract
Clinopodium alpinum subsp. orontium (K.Malý) Govaerts and Thymus bracteosus Vis. ex Benth. are endemic Lamiaceae species in Bosnia and Herzegovina with rather limited data about their cytotoxic and genotoxic effects. This study aimed to analyse phenolic compounds composition of C. alpinum subsp. orontium and T. bracteosus aqueous and dimethyl sulphoxide (DMSO) extracts and their cytotoxic and genotoxic potential in human peripheral blood lymphocyte cultures. Among 33 analytes, 17 were identified and quantified in the examined extracts with the rosmarinic and chlorogenic acids as main constituents. Genotoxic effects of extracts from both species are proven at the highest applied dose. T. bracteosus extracts and DMSO as a solvent exhibited stronger genotoxic potential that should be further investigated in tumour cell lines. Nevertheless, non-endemic species with similar phenolic composition and bioactivity should be the first choice for medicinal purposes.
- Published
- 2021
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