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Buçalışmada, Türkiye için endemik bir bitki olan Origanum acutidens (Hand.-Mazz.) Ietsw. iki farklı çözücü (Su ve %80 etanol) ile ekstrakte edildi. O.acutidens örnekleri Bingöl-Topalan'dan toplandı. (Toplayıcı kayıt numarası:MMH 1157). O. acutidens’in etanol vesu ekstraktlarının antioksidan aktiviteleri, toplam fenolik flavonoidiçeriklerinin yanı sıra element ve fenolik bileşik analizleri yapıldı. Tümanaliz sonuçları 1 mg/mL ekstrakt konsantrasyonuna göre hesaplandı. Analizsonuçlarına göre DPPH aktivitesinde; su ekstraktının % 77,53’ü etanolekstraktının ise % 90,69’u inhibisyon, FRAP aktivitesinde su için 0,34 mg/mL veetanol için 0,96 mg/mL FeSO4 eşdeğerinde aktivite gösterdiği tespitedildi. Toplam fenolik madde tayinindesu ekstraktının 86,48 mg /mL etanol ekstraktının ise 142,78 mg /mL gallik asiteşdeğeri total fenolik madde bulundu. Total flavonoid madde analizinde ise su ekstraktı için 280,58 mg/mL etanolekstraktı için ise 503,82 mg/mL rutin eşdeğeri flavonoid madde içeriği tespitedildi. Ayrıca O. acutidensbitkisinde 15 farklı fenolik bileşik ve 32 farklı element tespit edildi., In thisstudy, Origanum acutidens (Hand.-Mazz.)Ietsw. (zemul), an endemic plant forTurkey, were extracted with two different solvents (Water and 80 % ethanol). O. acutidens samples were collected fromBingöl-Topalan (Collector registration number: MMH 1157). In this study,antioxidant activities, total phenolic and flavonoid content, alsoidentification of element and phenolic compounds of O. acutidens (ethanol and water extracts) were analyzed. Allanalysis results were calculated at concentration of 1 mg/ml extract. Accordingto these results, for DPPH activity; It was found that the water extract showedactivity of 77.53% and ethanol extract 90.69% inhibition, at FRAP activity 0.34mg / mL for water and 0.96 mg / mL FeSO4 equivalent for ethanolextracts. Total phenolic content was calculated as 86.48 mg / mL in waterextract and 142.78 mg / mL gallic acid equivalent total phenolic substance inethanol extract. Total flavonoid analysis revealed 280.58 mg/mL for waterextract and 503.82 mg / mL routine equivalent flavonoid content for ethanolextract. In addition, 15 different phenolic compounds and 32 different elementswere detected in O. acutidens.

In this present study, it was aimed to determine the composition of the essential oil obtained by water distillation from the aerial parts of Origanum acutidens. The oil was analysed by GC-FID and GC/MS. Carvacrol (69.3%), p-cymene (8.3%), y-terpinene (6.3%), β-caryophyllene (2.7%) and borneol (2.2%) were found as main constituents. In addition, headspace-solid phase microextraction using a Polydimethylsiloxane-Divinylbenzene (PDMS/DVB – 65 μm) fiber of O. acutidens was performed and analysed by GC-FID and GC/MS. The main components were identified as: carvacrol (44.0%), p-cymene (25%), ϒ-terpinene (8%), β-caryophyllene (6.1%) and borneol (6.1%), respectively.

Aim: Nowadays, studies on the use of medicinal plants and their essential oils in cancer treatment are increasing rapidly and these studies are very important both scientifically and economically. However, the collection of plants from nature causes extinction. Therefore, it is of great importance to cultivate plants, especially endemic species, to reproduce and consume them. In our study, cytotoxic and membrane damaging effects of essential oils obtained from wild and cultured forms of Origanum acutidens (Hand.-Mazz.) Ietswaart (Lamiaceae) (O. acutidens), which is endemic to Turkey, on non-small-cell lung cancer (NSCLC) cells H1299 and A549 were compared. Material and Method: The level of malondialdehyde, an oxidative stress biomarker, was determined in cell lysates. Assessment of cell viability was made by CellTiter-Blue® Cell Viability Assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay after 10- 250 μg/mL concentrations of wild and cultured forms of O. acutidens essential oil treated to H1299 and A549 cells for 24, 48 and 72 h. Malondialdehyde levels were assayed for determining the membrane damaging effects. Results: Cell viability of H1299 and A549 cells incubated with essential oils obtained from wild and cultured forms of O. acutidens was found to decrease depending on concentration and time. 179 µg/mL, 157 µg/mL, and 132 µg/mL were calculated as IC50 values of wild form of O. acutidens essential oil on H1299 cells for 24, 48, and 72 h, respectively by MTT assay. 150 µg/mL, 131 µg/mL, and 110 µg/mL were calculated as IC50 values of wild form of O. acutidens essential oil on H1299 cells for 24, 48, and 72 h, respectively by resazurin-based assay. 118 µg/mL, 99 µg/mL, and 69 µg/mL were calculated as IC50 values of wild form of O. acutidens essential oil on A549 cells for 24, 48, and 72 h, respectively by MTT assay. 98 µg/mL, 83 µg/mL, and 57 µg/mL were calculated as IC50 values of wild form of O. acutidens essential oil on A549 cells for 24, 48, and 72 h, respectively by resazurin-based assay. Essential oils obtained from wild and cultured forms of O. acutidens increased malondialdehyde level on both H1299 and A549 cells. The greatest membrane damage was observed in A549 cells treated with wild form of O. acutidens essential oil. Conclusion: Essential oils obtained from wild and cultured forms of O. acutidens had cytotoxic effect on lung cancer cells and it has been demonstrated that they showed this effect by causing membrane damage in cells.

The word Origanum comes from the words that mean mountain ornament in Greek (oros= mountain, ganos = ornament)...

The main objective of the current work is to search the antioxidant activities of Origanum acutidens leaves by using DPPH, FRAP and CUPRAC methods severally. Three well known antioxidant compounds (BHA, BHT and ascorbic acid) were used as standards for comparing the extracts. Also, phenolic compounds of Origanum acutidens leaves were identified by UHPLC-ESI-MS/MS. The high concentrations of rosmarinic acid (11158.99 ppb) quinic acid (3200.84 ppb) and naringenin (1238.45 ppb) were detected quantitatively.

Objectives: The aim of this study was to investigate the cytotoxic and radical scavenging effects of the essential oil from Origanum acutidens (Hand-Mazz.) Ietswaart and to determine its chemical composition. Materials and Methods: The essential oil was obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC-MS). The cytotoxic effect of the essential oil on the growth of human colorectal adenocarcinoma (HT-29) and human cervical adenocarcinoma (HeLa) cell lines was investigated by xCELLigence Real Time Cell Analyzer instrument. Moreover, radical scavenging effect of the oil was determined by using DPPH method. Results: The main constituents of the oil were found to be carvacrol (61.69%), p-cymene (17.32%), γ-terpinene (4.05%), and borneol (3.96%). The essential oil of O. acutidens exhibited significant cytotoxic effect against HeLa and HT-29 cell lines at the tested concentrations. The essential oil had moderate DPPH radical scavenging activity compared to butylated hydroxytoluene (BHT). Conclusion: The data in the present study clearly demonstrated inhibitory effect of the oil on two human cancer cell lines. According to these results and other reported studies, this observed high effect may be attributed to the presence of the carvacrol component in the oil.

The chromosome number and chromosome morphology of endemic species Centaurea fenzlii and Origanum acutidens naturally growing in Turkey were investigated using karyological techniques. The specimens were collected from natural habitats in the years 2013. The basic chromosome number of Centaurea fenzlii is: x= 9 and diploid chromosome numbers were found to be 2n=18, species of Origanum acutidens basic chromosome number is: x= 15 and diploid chromosome numbers were found to be 2n=30. Chromosomes had median region (m) and submedian (sm) centromers. Karyotype analysis of Origanum acutidens endemic species has been conducted by us for the first time. © Afyon Kocatepe Üniversitesi, Türkiye’ de doğal olarak yetişen Centaurea fenzlii Reichardt. ve Origanum acutidens (Hand.-Mazz.) Ietswaart. endemik türlerinin kromozom sayısı ve kromozom morfolojisi karyolojik teknikler kullanılarak araştırıldı. Bitki örnekleri 2013 yılında doğal habitatlarından toplandı. Çalışılan bu türlerden Centaurea fenzlii’ nin temel kromozom sayısı x=9 ve diploid kromozom sayısı ise 2n=18, Origanum acutidens türünün temel kromozom sayısı x=15 ve diploid kromozom sayısı ise 2n=30 olarak tespit edildi. Türlere ait kromozomların median (m) ve submedian (sm) sentromerli olduğu gözlendi. Origanum acutidens endemik türünün karyotip analizleri ilk olarak tarafımızdan yapıldı

Objectives: The aim of this study was to investigate the cytotoxic and radical scavenging effects of the essential oil from Origanum acutidens (Hand-Mazz.) Ietswaart and to determine its chemical composition. Materials and Methods: The essential oil was obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC-MS). The cytotoxic effect of the essential oil on the growth of human colorectal adenocarcinoma (HT-29) and human cervical adenocarcinoma (HeLa) cell lines was investigated by xCELLigence Real Time Cell Analyzer instrument. Moreover, radical scavenging effect of the oil was determined by using DPPH method. Results: The main constituents of the oil were found to be carvacrol (61.69%), p-cymene (17.32%), γ-terpinene (4.05%), and borneol (3.96%). The essential oil of O. acutidens exhibited significant cytotoxic effect against HeLa and HT-29 cell lines at the tested concentrations. The essential oil had moderate DPPH radical scavenging activity compared to butylated hydroxytoluene (BHT). Conclusion: The data in the present study clearly demonstrated inhibitory effect of the oil on two human cancer cell lines. According to these results and other reported studies, this observed high effect may be attributed to the presence of the carvacrol component in the oil.

The chemical constituents of essential oil by hydrodistillation from the aerial parts of Hyssopus officinalis L. and Origanum acutidens (hand.-mazz.) ietswaart from Turkey were identified by gas chromatograph-mass spectrometer (GC-MS) demonstrated the presence of at least 24 compounds. The major constituents of H. officinalis oil were pinocarvone (29.2 %), trans-pine camphone (27.2 %), β-pinene (17.6 %), cis-pinocamphone (4.7 %) and myrcene (2.92 %). The major constituents of the essential oil of O. acutidens were carvacrol (76.2 %), p-cymene (7.4 %) and borneol (3.2 %).

WOS: 000278400600031, PubMed ID: 20438322, The radical scavenging, antimicrobial, and antispasmodic activities and the composition of Origanum acutidens (Hand.-Mazz.) Ietswaart (Order Labiatte) essential oil were evaluated in vitro. Gas chromatography/mass spectrometry analysis of the oil resulted in the identification of 20 compounds, representing 93.61% of the oil; carvacrol (65%) was the main component. The sample was also subjected to a screening for antioxidant activity by using the 1,1-diphenyl-2-picrylhydrazyl and beta-carotene/linoleic acid assays. The essential oil exhibited strong antioxidant activity. Using the agar disk diffusion method, antimicrobial activities of the essential oils were determined for nine microorganisms; antimicrobial activity was shown against eight of them. O. acutidens oil exhibited spontaneous contractions in rat ileum at the 100% level at 0.1 mg/mL level.

OZER, Hakan/0000-0002-8788-1597; Tozlu, Goksel/0000-0002-7187-7825; TOZLU, Elif/0000-0002-0016-9696; Cakir, Ahmet/0000-0003-1672-1438 WOS: 000294942900002 The essential oils of aerial parts of Achillea gypsicola Hub.-Mor., Hypericum scabrum L, Satureja hortensis L, and Origanum acutidens (Hand.-Mazz.) Letswaart were analyzed in this study by GC and GC-MS and their oils were tested for toxicity against broadbean weevil (Bruchus dentipes). A. gypsicola oil contained camphor (40.17%), 1,8-cineole (22.01%), piperitone (11.29%), borneol (9.50%) and alpha-terpineol (1.56%) as major components. A total of 74 components were identified by GC-MS in H. scabrum oil. including a-pinene (9.26%), terpinen-4-ol (5.12%), camphor (5.94%), delta-cadinene (4.52%), pulegone (4.45%),), gamma-muurolene (4.12%), pinocarvone (3.97%) and beta-caryophyllene (3.42%) as predominant components. The essential oils of O. acutidens and S. hortensis were characterized by high contents of carvacrol (86.99% and 55.74%), gamma-terpinene (0.71% and 20.94%), p-cymene (1.95% and 12.30%), alpha-terpinene (0.13% and 2.04%) and beta-caryophyllene (1.30% and 1.08%). All of the essential oils were toxic to adults of B. dentipes and insect mortality increased with increasing concentration of each oil. The oils (20 pi dose) brought about 100% mortality in 36 h. Although desirable insecticidal activities against the pest were achieved with the oils from all four plant species, S. hortensis and O. acutidens oils were more effective, particularly after 6 h of treatment. The current results concluded that the essential oils, in particular O. acutidens and S. hortensis oils, may be used as potential botanical insecticides against B. dentipes. (C) 2011 Elsevier B.V. All rights reserved.

WOS: 000221652400015, PubMed ID: 15161188, The essential oil and various extracts obtained from Origanum acutidens and methanol extracts (MeOH) from callus cultures have been evaluated for their antioxidative, antimicrobial, and antiviral properties. The essential oil exhibited strong antimicrobial activity with a significant inhibitory effect against 27 (77%) of the 35 bacteria, 12 (67%) of the 18 fungi, and a yeast tested and moderate antioxidative capacity in DPPH and beta-carotene/linoleic acid assays. GC and GC-MS analyses of the oil resulted in the identification of 38 constituents, carvacrol being the main component. The MeOH extracts obtained from herbal parts showed better antioxidative effect than that of butylated hydroxytoluene (BHT), whereas callus cultures also exhibited interesting antioxidative patterns. Concerning antiviral activity, none of the extracts inhibited the reproduction of influenza A/Aichi virus in MDCK cells. The MeOH extracts from herbal parts inhibited the reproduction of HSV-1, and also callus cultures exerted slight antiherpetic effect.

WOS: 000322750600010, PubMed ID: 23613392, Purpose: There has been a long-standing interest in the identification of medicinal plants and derived natural products for developing anticancer agents. This work aimed at investigating the antiprolipherative properties of Origanum acutidens (OA) on breast cancer. Methods: OA water extracts were studied for cytotoxicity against the breast cancer cell lines MCF-7, MDA-MB-468 and MDA-MB-231. In vitro apoptosis studies of these cancer cell lines were performed by annexin V staining in flow cytometry analyses. Immunohistochemistry studies for Ki-67 and caspase-7 of tumor tissue sections of dimethylbenzanthracene (DMBA) -induced mammary cancer in rats were also performed. TUNEL assay was used to detect apoptotic cells of tumor tissue. In vivo anticancer activity testing was carried out by inhibiting the growth of DMBA-induced mammary cancer in rats. Results: OA showed cytotoxicity on all 3 cancer cell lines. Annexin-positive cells level in OA-treated cell lines were significantly higher compared with untreated control cells (p=0.002). The expressions of caspase-7 protein and TUNEL-positive cells were much higher for the rats treated by OA, compared with the untreated control group (p, Scientific Research Project Fund of Cumhuriyet University [T-383], This work has been supported by the Scientific Research Project Fund of Cumhuriyet University under the project number T-383.

Water-distilled essential oil of Origanum acutidens, an endemic species of Turkey, was analyzed by GC and GC/MS. Forty-six components constituting 99.04% of the total components detected were characterized. Carvacrol (66.25%) and p-cymene (13.99%) were identified as major components.