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25,731 results on '"Oral Medicine"'

1. Radiographical Assessment of Mandibular Molars of children and young Adults to determine the probability at 16-year threshold: Sri Lankan study

Author

Ranasinghe, Forensic Odontologist Dr. Sisira, Perera, Professor of Forensic Medicine and Toxicology Professor Jean, Taylor, Professor of Oral Biology Professor Jane A., Tennakoon, Forensic Pathologist Dr. Ajith, Pallewatte, Consultant Radiologist Dr. Aruna, and Jayasinghe, Professor of Oral Medicine Professor Ruwan

Published
2022
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4. Book of abstracts of the congress “120 Years of Organized Dental Medicine in the Republic of Croatia” and symposium “Oral Medicine and Medically Complex Patients for General Dental Practice”, with international participation November 10-11, 2023, Zagreb, Croatia

Author

120 Years of Organized Dental Medicine in the Republic of Croatia, Congress, Oral Medicine and Medically Complex Patients for General Dental Practice, Symposium, 120 Years of Organized Dental Medicine in the Republic of Croatia, Congress, and Oral Medicine and Medically Complex Patients for General Dental Practice, Symposium

Published
2023

5. Oral medicine (stomatology) across the globe: birth, growth, and future

Author

Scully, Crispian, Miller, Craig S., Aguirre Urizar, Jose-Manuel, Alajbeg, Ivan, Almeida, Oslei P.D., Bagan, Jose Vicente, Birek, Catalena, Chen, Qianming, Farah, Camile S., Figueirido, José Pedro, Hasséus, Bengt, Jontell, Mats, Kerr, A. Ross, Laskaris, George, Lo Muzio, Lorenzo, Mosqueda-Taylor, Adalberto, Nagesh, Kikkeri S., Nikitakis, Nikolaos G., Peterson, Douglas, Sciubba, James, Thongprasom, Kobkan, Tovaru, Şerban, and Zadik, Yehuda

Published
2016
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6. Awareness of Teledentistry Amongst Dental Professionals in India Amid COVID-19 Pandemic. A Cross-Sectional Study

Author

Nigam, Haritma; Department of Oral Medicine and Radiology, Pacific Dental College and Research Center, Udaipur, Rajasthan, Shah, Takshil D.; Department of Prosthodontics, Gujarat University, Ahmedabad - 380013, Gujarat, Saikia, Priyanki; Consultant Endodontist, Dental Concept, Guwahati, Assam, Raza, Aasif; Dental Surgeon, Ministry of Health & Family welfare, Girdih, Jharkhand, Gupta, Ankita; Department of Oral Medicine & Radiology, Inderprastha Dental College & Hospital, New Delhi, Prapurna, Kalyani; Consultant Periodontist, Conquer Hospital, Andhra Pradesh, Nigam, Haritma; Department of Oral Medicine and Radiology, Pacific Dental College and Research Center, Udaipur, Rajasthan, Shah, Takshil D.; Department of Prosthodontics, Gujarat University, Ahmedabad - 380013, Gujarat, Saikia, Priyanki; Consultant Endodontist, Dental Concept, Guwahati, Assam, Raza, Aasif; Dental Surgeon, Ministry of Health & Family welfare, Girdih, Jharkhand, Gupta, Ankita; Department of Oral Medicine & Radiology, Inderprastha Dental College & Hospital, New Delhi, and Prapurna, Kalyani; Consultant Periodontist, Conquer Hospital, Andhra Pradesh

Abstract

Background: COVID-19 has become a crisis across the country these days, which likewise changed living of individual to entity contact and its spread. There the presentation of Teledentistry will be the solitary solution that will have the option to treat patients quickly and expertly. Aim: The current study aims to assess the awareness of dental professionals towards teledentistry during COVID-19 crisis in India. Methods: A cross-sectional descriptive survey was conceded on over all 142 dentists. A self-organized, close-ended questionnaire containing 20 questions was appropriately assembled and distributed. Data collected from the survey was examined in terms of frequency (yes/no). One Way Anova test was used to exemplify statistical variances and result came as significant at p < 0.05. Results: The responses from specialist dental professionals (with or without practicing) responded (57%) and dentist working in a private sector (68%) having clinical experience of about 5-10 years (36%). Conclusion: Dentists were well aware of this advanced technique and due to feasibility, most of them were ready to practice it in future.

Published
2021

7. Primary Non-Hodgkin’s Lymphoma of the Mandible in an Immunocompetent Patient

Author

Arora, Preeti Chawla; Associate Professor, Department of Oral Medicine, Diagnosis and Radiology, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, Arora, Aman; Associate Professor, Department of Prosthodontics and Crown and Bridge and Implantology, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, Ramaswami, Easwaran; Associate Professor, and Head, Department of Oral Medicine and Radiology, Government Dental College and Hospital, Mumbai, Maharashtra, Arora, Saurabh; Senior Resident, Department of Pathology, GMC, Amritsar – 143001, Punjab, Arora, Preeti Chawla; Associate Professor, Department of Oral Medicine, Diagnosis and Radiology, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, Arora, Aman; Associate Professor, Department of Prosthodontics and Crown and Bridge and Implantology, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, Ramaswami, Easwaran; Associate Professor, and Head, Department of Oral Medicine and Radiology, Government Dental College and Hospital, Mumbai, Maharashtra, and Arora, Saurabh; Senior Resident, Department of Pathology, GMC, Amritsar – 143001, Punjab

Abstract

Non-Hodgkin’s Lymphoma (NHL) is very commonly associated with immunosuppressive states. Its occurrence in an immunocompetent person is scarcely reported in literature. Although NHL is known to occur in the jaws, mandibular involvement with invasion in bone is an uncommon entity. Dental surgeons should be aware that NHL can present in the oral cavity as a non-tender swelling or ulceration and should be included in the differential diagnosis of oral swellings and ulcers. Histopathological examination, assessment of immune status of the patient and search for primary or metastatic lesions should be a part of the diagnostic work up. A case of NHL of the mandible in an immunocompetent patient with follow up is presented here.

Published
2020

8. Extraoral Sublingual Epidermoid Cyst: A Case Report

Author

Limdiwala, Piyush G.; Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, Shah, Jigna S.; Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, Parikh, Shilpa J.; Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, Chaudhari, Nayankumar K.; Department of Dentistry, R.H and C.H.C, Gadat, Tapi, Vyara, Gujarat, Limdiwala, Piyush G.; Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, Shah, Jigna S.; Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, Parikh, Shilpa J.; Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, and Chaudhari, Nayankumar K.; Department of Dentistry, R.H and C.H.C, Gadat, Tapi, Vyara, Gujarat

Abstract

Epidermoid Cysts (EC) are rare cysts of the head and neck region, which may confuse with many soft tissue sublingual mass pathologies. A presentation of such cyst is important to decide the surgical approach. The aim of this paper was to present the role of USG and MRI for determine the surgical approach. A female patient presented with chronic asymptomatic swelling over left submandibular neck which was single diffuse, non-tender, soft, compressible, fluctuant with normal overlying skin. Swelling was not movable with deglutination and protrusion of tongue. MRI and USG guided FNAC was conclusive to diagnose the lesion. Histological examination confirmed the diagnosis. Lateral neck swelling should be considered as EC for differential diagnosis. A careful physical examination and fine needle aspiration and diagnostic imaging should be performed which clarifies the surgical approach.

Published
2020

9. Radiographical Assessment of Mandibular Molars of children and young Adults to determine the probability at 16-year threshold: Sri Lankan study

Author

Forensic Odontologist Dr. Sisira Ranasinghe, Professor of Forensic Medicine and Toxicology Professor Jean Perera, Professor of Oral Biology Professor Jane A. Taylor, Forensic Pathologist Dr. Ajith Tennakoon, Consultant Radiologist Dr. Aruna Pallewatte, and Professor of Oral Medicine Professor Ruwan Jayasinghe

Subjects
Radiology, Nuclear Medicine and imaging, Pathology and Forensic Medicine
Published
2022

10. Embryonal Rhabdomyosarcoma as a Gingival Growth: A Rare Presentation

Author

Shah, Jigna S.; Professor and HOD, Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, Lunagriya, Nutan; Post Graduate Student, Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, Shah, Jigna S.; Professor and HOD, Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat, and Lunagriya, Nutan; Post Graduate Student, Department of Oral Medicine and Radiology, Government Dental College and Hospital, Asarwa, Ahmedabad - 380016, Gujarat

Abstract

Rhabdomyosarcoma (RMS) is a malignant tumor of striated muscle composed of neoplastic mesenchymal cells with varying degrees of cell differentiation. It accounts 60% of all soft-tissue sarcomas in children and only 2-5% in adults. Typical pediatric rhabdomyosarcoma variants like embryonal and alveolar sub-types are less frequently occur in adults. It has predilection for viscera followed by the head and neck region. RMS occurs in the head and neck region with 35% and 10-12% in the oral cavity. Establishment of the correct diagnosis and histological sub-type is of very importance in the treatment of this disease, because of the rarity of this neoplasm in the adult population the treatment is not uniform in the literature. Here we present case of embryonal rhabdomyosarcoma as a gingival growth in a 26-year-old female with clinical, radiological, histo-pathological and immunohistochemical aspects.

Published
2019

11. Prevalence of Haller’s Cells using Computed Tomography in Namakkal District - A Retrospective Study

Author

Mohan, Chitra; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, Mani, Sudhaa; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, Pazhani, Ambiga; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, G., Sivaraman; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, Ahamed, Yasmeen; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, N., Balan; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, Mohan, Chitra; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, Mani, Sudhaa; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, Pazhani, Ambiga; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, G., Sivaraman; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, Ahamed, Yasmeen; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205, and N., Balan; Department of Oral Medicine, Vivekanandha Dental College for Women, Namakkal district, Tamil Nadu - 637205

Abstract

Aim: Haller’s cells are developmental variants of anterior ethmoidal sinus which are also known as infraorbital ethmoidal cells or orbito ethmoidal cells or maxilla ethmoidal cells. These anatomic variants of sinonasal regions pneumatize inferiorly and medial to the orbital floor and towards the inferior of maxillary sinus. Hence, the present study was to determine the prevalence of Haller's cells using CT PNS images. Objectives: The purpose of the study is to determine the prevalence of Haller’s cells according to age and gender, to evaluate the relationship between Haller’s cells and maxillary sinusitis and to correlate between Haller’s cells and ipsilateral orbital floor dehiscence. Methods: CT PNS images of 60 patients within the age group of 16-75 years were retrieved, screened by a radiologist and analyzed. The data collected were subjected to statistical analysis analyzed with statistical software package, SPSS version 16.0. and p value < 0.05 was considered to be statistically significant. Results: The prevalence of Haller's cells in the present study was found to be 48%. There was no statistically significant association between the presence of Haller cells with respect to age and gender. There was a significant association between Haller cells with maxillary sinusitis and orbital floor dehiscence. Conclusion: This study has attempted to find the prevalence of Haller’s cells which will be helpful for dentists in specifying the differential diagnosis for Oro facial pain and maxillary sinusitis.

Published
2019

12. Dens Evaginatus and Dens Invaginatus in All Maxillary Incisors A Case Report with Review of Literature

Author

Kurian, K.; Professor, Department of Oral Medicine & Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, Shanmugam, S.; Professor and HOD, Department of Oral Medicine & Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, Vishwanath, R.; Reader, Department of Oral Medicine & Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, T., Harsha Vardhan; P. G. Student, Department of Oral Medicine & Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, Kurian, K.; Professor, Department of Oral Medicine & Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, Shanmugam, S.; Professor and HOD, Department of Oral Medicine & Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, Vishwanath, R.; Reader, Department of Oral Medicine & Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, and T., Harsha Vardhan; P. G. Student, Department of Oral Medicine & Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu

Abstract

Dens evaginatus is a developmental malformation characterized by the presence of an extra cusp that takes the form of a tubercle arising from the occlusal or the lingual surface of the tooth'. It is also refer red to as talons cusp in the anterior teeth and enamel pearl in the premolars. Dens invaginatus is a deep surface invagination of the crown or the root that is lined by enamel, dentin and pulp. Though many cases of dens evaginatus and dens invaginatus have been reported in literature, an association of both is a rare anomaly. This is a case report of dens evaginatus and dens Invaglnatus 'Involving all the four maxillary incisors with bilateral paramolars in a 25 yr old patient.

Published
2018

13. Spindle Cell Carcinoma - A Case Report

Author

Mubeen, ; Professor & HOD, Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Bangalore, Karnataka, Shilpa, Yalsangi; PG students, Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Bangalore, Karnataka, Eugenia, Sherubin J.; Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Bangalore, Karnataka, Mubeen, ; Professor & HOD, Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Bangalore, Karnataka, Shilpa, Yalsangi; PG students, Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Bangalore, Karnataka, and Eugenia, Sherubin J.; Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Bangalore, Karnataka

Abstract

Spindle cell carcinoma is a relatively rare malignant neoplasm. It occurs primarily in the oral cavity and larynx and less often in the Esophagus and skin. The mean age at diagnosis for Spindle cell carcinoma is 57 years. This paper presents the case of a 17 year old girl with Spindle cell carcinoma involving the left maxillary alveolus. P.D of CGCG and D/D of minor salivary gland tumor, Ca-alveolus, Caantrum was considered. Histopathological and imunohistochemical analysis confirmed a diagnosis of Spindle cell carcinoma.

Published
2018

14. Embryonal Rhabdomyosarcoma of Oral Cavity with Extensive Osteolysis of the Mandible – A Rare Case Report

Author

Jayaram, Vidya; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Tamil Nadu, Jayachandran, S.; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Tamil Nadu, Bhaskar, Y. Hemavathy; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Tamil Nadu, Jayaram, Vidya; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Tamil Nadu, Jayachandran, S.; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Tamil Nadu, and Bhaskar, Y. Hemavathy; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Tamil Nadu

Abstract

Rhabdomyosarcoma is a malignant tumor of skeletal muscle. It is the most common soft tissue sarcoma in children and adolescents. It rarely occurs in oral cavity and accounts for 0.04% of all head and neck malignancies. The incidence is 4.5 per 1 million children and 50% of cases are seen in the first decade of life. In this paper, we report a rare case of oral rhabdomyosarcoma in a 3 year old boy with involvement of buccal mucosa and extensive destruction of the mandible.

Published
2018

15. Benign Cementoblastoma - A Rare Odontogenic Tumour

Author

Aswath, Nalini; Department of Oral Medicine and Radiology, Sree Balaji Dental College & Hospital, Chennai, Tamil Nadu, Balaji, Anandhi; Department of Oral Medicine & Radiology, SRM Dental College, Ramavaram, Chennai, Aswath, Nalini; Department of Oral Medicine and Radiology, Sree Balaji Dental College & Hospital, Chennai, Tamil Nadu, and Balaji, Anandhi; Department of Oral Medicine & Radiology, SRM Dental College, Ramavaram, Chennai

Abstract

Benign cementoblastoma is a rare odontogenic tumour derived from odontogenic ectomesenchyme of cementoblast origin that forms cementum layer on the roots of a tooth. Benign cementoblastomas have a distinct clinical and radiographic appearance. It is important to consider them as one among the differential diagnosis in bony swellings of mandible. A case report of a 55 yr old female patient diagnosed as a case of benign cementoblastoma is presented.

Published
2018

16. Craniofacial Polyostotic Fibrous Dysplasia: Report of A Case with Review of Literature

Author

Mubeen, ; Professor and head of the Department, Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Fort, Bangalore, Karnataka, K. R., Kiran Kumar; Postgradllate Student, Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Fort, Bangalore, Karnataka, Mubeen, ; Professor and head of the Department, Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Fort, Bangalore, Karnataka, and K. R., Kiran Kumar; Postgradllate Student, Department of Oral Medicine & Radiology, Government Dental College & Research Institute, Fort, Bangalore, Karnataka

Abstract

Fibrous dysplasia is a nonspecific hamartomatous skeletal developmental anomaly, characterized by slow progressive replacement of a localized area of bone by an abnormal proliferation of isomorphic fibrous tissue. The exact etiology of it is in ambiguity. The recent concept with etiopathogenesis proposes that post-zygotic mutation in the guanine nucleotide binding protein a-stimulating activity polypeptide-l gene. The Fibrous dysplasia can be of monostotic type which is common and affects maxilla, and polyostotic type, a rare variety. The incidence of cranial fibrous dysplasia is even rare amongst the primary bone lesions, representing less than one percent. There is no specific treatment for fibrous dysplasia. Untreated fibrous dysplasias are associated with complications like hearing loss, trismus, and facial disfigurement for which cosmetic surgical treatment is rendered.

Published
2018

17. Odontoma-associated Calcifying Cystic Odontogenic Tumour: Report af A Case

Author

Arora, Preeti Chawla; Department of Oral Medicine, Diagnosis & Radiology, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, Umarji, H. R.; Department of Oral Medicine, Diagnosis & Radiology, Government Dental College and Hospital, Mumbai, Maharashtra, Kaur, Arshdeep; Department of Oral Medicine, Diagnosis & Radiology, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, Arora, Aman; Department of Prosthodontics, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, Arora, Preeti Chawla; Department of Oral Medicine, Diagnosis & Radiology, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, Umarji, H. R.; Department of Oral Medicine, Diagnosis & Radiology, Government Dental College and Hospital, Mumbai, Maharashtra, Kaur, Arshdeep; Department of Oral Medicine, Diagnosis & Radiology, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab, and Arora, Aman; Department of Prosthodontics, SGRD Institute of Dental Sciences and Research, Amritsar, Punjab

Abstract

The calcifying cystic odontogenic tumour (CCOT) is an uncommon benign neoplasm of odontogenic origin, which was first described by Gorlin. It has been shown to have extensive diversity in its clinical and histopathological features and its biological behaviour. Most of these cases present with cystic characteristics, but a few are of the solid type. Cystic variant of CCOT associated with odontoma is considered as rare variant. A case of calcifying cystic odontogenic tumour associated with odontoma is reported in an 11-yearold female patient. In this report, the lesion was very large extending from the midline up to the right tuberosity region and impeded the eruption of five permanent teeth. Through this article, the radiographic and histopathological features of this entity are discussed and the literature is reviewed.

Published
2018

18. A Case of Isolated Craniofacial Fibrous Dysplasia - Radiologist's Perspective

Author

Regu, P.; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Jayachandran, S.; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai, 600003, Meenapriya, P. K.; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Regu, P.; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003, Jayachandran, S.; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai, 600003, and Meenapriya, P. K.; Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital, Chennai 600003

Abstract

Fibrous dysplasia is a disease characterized by replacement of bone by fibro osseous tissue. FD has four different disease patterns. They are monostotic, polyostotic and McCunee Albright syndrome. Craniofacial pattern of disease occurs in 10-25% of patients with monostotic form and in 50% with polyostotic form. It also occurs as isolated craniofacial form. In isolated variety no extracranial lesions are present. Radiographic diagnosis plays an important role in diagnosis, classification and assessing prognosis of fibrous dysplasia. In this paper we report a case of isolated craniofacial type of fibrous dysplasia in a young female patient involving the maxilla and skull bones with a complete radiographic CT assessment of the extent of the lesion. Temporal bone involvement and bilateral lesion in certain cranial bones are the rare findings noted in this case.

Published
2018

19. Comparing the Effect of Coconut Oil Pulling Practice with Chlorhexidine Mouth Wash in Plaque Induced Gingivitis by Evaluation of Salivary Biochemical Marker – A Comparative Interventional Study

Author

Kaliamoorthy, Sriram; Department of Dentistry, Vinayaka Missions Medical College, Vinayaka Mission's Research Foundation (Deemed to be University), Karaikal – 609 609, Puducherry, Vijayakumar, Jayasuriya; Department of Dentistry, Vinayaka Missions Medical College, Vinayaka Mission's Research Foundation (Deemed to be University), Karaikal – 609 609, Puducherry, Caliaperoumal, Santhosh Kumar; Department of Dentistry, Vinayaka Missions Medical College, Vinayaka Mission's Research Foundation (Deemed to be University), Karaikal – 609 609, Puducherry, Pazhani, Ambiga; Department of Oral Medicine and Radiology, Vivekananda Dental College and Hospital, Thiruchencode – 637 205, Tamilnadu, Raju, Kanmani; Department of Oral Medicine & Radiology, Chettinad Dental College and Research Institute, Kelambakkam – 603 103, Tamilnadu, Venkatesan, Padmavathi; Department of Oral Medicine & Radiology, Chettinad Dental College and Research Institute, Kelambakkam – 603 103, Tamilnadu, Murugaboopathy, Vikneshan; Department of Public Health Dentistry, Indira Gandhi Institute of Dental Sciences, Sree Balaji Vidhyapeeth (Deemed to be University), Puducherry – 607 403, Kaliamoorthy, Sriram; Department of Dentistry, Vinayaka Missions Medical College, Vinayaka Mission's Research Foundation (Deemed to be University), Karaikal – 609 609, Puducherry, Vijayakumar, Jayasuriya; Department of Dentistry, Vinayaka Missions Medical College, Vinayaka Mission's Research Foundation (Deemed to be University), Karaikal – 609 609, Puducherry, Caliaperoumal, Santhosh Kumar; Department of Dentistry, Vinayaka Missions Medical College, Vinayaka Mission's Research Foundation (Deemed to be University), Karaikal – 609 609, Puducherry, Pazhani, Ambiga; Department of Oral Medicine and Radiology, Vivekananda Dental College and Hospital, Thiruchencode – 637 205, Tamilnadu, Raju, Kanmani; Department of Oral Medicine & Radiology, Chettinad Dental College and Research Institute, Kelambakkam – 603 103, Tamilnadu, Venkatesan, Padmavathi; Department of Oral Medicine & Radiology, Chettinad Dental College and Research Institute, Kelambakkam – 603 103, Tamilnadu, and Murugaboopathy, Vikneshan; Department of Public Health Dentistry, Indira Gandhi Institute of Dental Sciences, Sree Balaji Vidhyapeeth (Deemed to be University), Puducherry – 607 403

Abstract

Background: Oil pulling is an oral hygiene practice and is described in the ancient Ayurvedic literature. Materials and Methods: A total of 30 subjects with plaque related mild – moderate gingivitis in the age Group of 18-25 was recruited and they were divided into two Groups as Group A and B, each with 15 subjects. Group A subjects were advised to practice coconut oil pulling therapy and Group B subjects were advised to use Chlorhexidine Gluconate 0.12% solution for 4 weeks as an adjuvant practice along with routine tooth brushing. Estimation of salivary Aspartate Aminotransferase was done at pre and post intervention stage for both the study Groups by using semi auto analyser. Results: Statistically significant reduction in the reduction in the salivary Aspartate Aminotransferase level was seen at post intervention stage in Group A and Group B compared to preintervention stage. Comparison of salivary Aspartate Aminotransferase level between Group A subjects and Group B subjects at pre and post intervention stage revealed no significant difference. Our study results show that oil pulling utilizing coconut oil is effective in reducing the severity of plaque induced mild-moderate gingivitis and the effect is comparable to 0.12% chlorhexidine mouthwash in reducing plaque formation and gingivitis. Conclusion: Oil pulling utilizing coconut oil is an effective adjuvant oral hygiene practice in reducing the severity of plaque induced mild – moderate gingivitis along with the routine oral hygiene measure. Coconut oil pulling practice could be a potential alternative to chlorhexidine mouth wash for long term use.

Published
2018

20. Awareness, Attitude & Knowledge of COVID-19 amongst North Indian Population: Mirror of an Outburst Scenario

Author

Nigam, Haritma; Department of Oral Medicine and Radiology, Pacific Dental College and Research Center, Udaipur, Rajasthan, Shah, Takshil D.; Department of Prosthodontics, Gujarat University, Ahmedabad - 380013, Gujarat, Bhardwaj, Sudhanshu; Private Practioner, Periodontology, Jaipur, Rajasthan, Parmar, Pravin; Department of Dentistry, SMIMER Medical College, Surat, Gujarat, Raza, Aasif; Dental Surgeon, Ministry of Health and Family Welfare, Jharkhand, Saware, Sandhya; Private Practitioner, Prosthodontics, Mumbai, Maharashtra, Nigam, Haritma; Department of Oral Medicine and Radiology, Pacific Dental College and Research Center, Udaipur, Rajasthan, Shah, Takshil D.; Department of Prosthodontics, Gujarat University, Ahmedabad - 380013, Gujarat, Bhardwaj, Sudhanshu; Private Practioner, Periodontology, Jaipur, Rajasthan, Parmar, Pravin; Department of Dentistry, SMIMER Medical College, Surat, Gujarat, Raza, Aasif; Dental Surgeon, Ministry of Health and Family Welfare, Jharkhand, and Saware, Sandhya; Private Practitioner, Prosthodontics, Mumbai, Maharashtra

Abstract

Context: In the current era, the evolving pathogen, CoVs was originated anonymously in a patient suffering from pneumonia in Wuhan in December 2019 has drawn implausible care around the world. Aim: Its aim was to assess the knowledge, attitudes and degree of awareness among the North India population of different ages towards COVID-19 through a well-formatted questionnaire. Methods and Materials: A cross-sectional study of 384 participants enlisted from various places was conducted through an effective and dependable questionnaire including socio demographic and COVID-19 knowledge data in the population of Northern India. Statistical Analysis Used: ONE-WAY ANOVA test was used to illustrate statistical differences and result came as significant at (p<0.05). Result: Maximum participants were males (52%) of age 40-59 years (39%); secondary passed (12%) and people working in a private sector (12%). Conclusion: Due to collaboration of all the sources as social media, television, newspaper and health related advertisement public was having good knowledge about COVID-19.

Published
2020

22. Symptomatic Bilateral Bifid Mandibular Condyle and Oligodontia in a Non-syndromic Patient - A Rare Case Report and Review of Literature

Author

Chaudhry, Astha; Department of Oral Medicine and Radiology, Faculty of Dental Sciences, SGT University, Gurgaon – 122505, Haryana, Sharma, Akshika; Department of Health Behaviour, Roswell Park Comprehensive Cancer Center, Buffalo, New York, Chaudhry, Astha; Department of Oral Medicine and Radiology, Faculty of Dental Sciences, SGT University, Gurgaon – 122505, Haryana, and Sharma, Akshika; Department of Health Behaviour, Roswell Park Comprehensive Cancer Center, Buffalo, New York

Abstract

Bifidism of mandibular condyle is an uncommon anomaly of unknown etiology and uncertain pathogenesis. Usually it is asymptomatic and detected incidentally on a panoramic radiograph. However, it may be symptomatic where panoramic radiograph should be supplemented with computed tomography or magnetic resonance if surgical treatment is planned. The literature has reviewed many cases of bifid condyle mostly unilateral. The association of other dental abnormalities associated with bifid condyle has not been reported. We present a rare case of bilateral bifid condyle along with anterior disc displacement with reduction which also showed multiple missing teeth and retained deciduous teeth. This case report signifies the importance of both developmental and occlusal factors as an etiology for symptomatic temporomandibular joint.

Published
2019

23. Oral Cancer Screening Based on Methylation Frequency Detection in hTERT Gene Using Electrochemical Hybridization Assay via a Multi‐electrode Chip Coupled with Ferrocenylnaphthalene Diimide

Author

Department of Science of Physical Functions, Division of Oral and Maxillofacial Surgery, Kyushu Dental University, 2-6-1 Manazuru, Kokurakitaku, Kitakyushu, Fukuoka 803-8580, Japan, Department of Applied Chemistry and Research Center for Bio-microsensing Technology, Kyushu Institute of Technology,1-1 Sensui, Tobataku, Kitakyushu, Fukuoka 804-8550, Japan, Department of Health Promotion, Division of Oral Pathology, Kyushu Dental University, 2-6-1 Manazuru, Kokurakitaku, Kitakyushu, Fukuoka 803-8580, Japan, Department of Science of Physical Functions, Division of Oral Medicine, Kyushu Dental University, 2-6-1 Manazuru, Kokurakitaku, Kitakyushu, Fukuoka 803-8580, Japan, Haraguchi, Kazuya, Sato, Shinobu, Habu, Manabu, Yada, Naomi, Hayakawa, Mana, Takahashi, Osamu, Yoshioka, Izumi, Matsuo, Kou, Tominaga, Kazuhiro, Takenaka, Shigeori, Department of Science of Physical Functions, Division of Oral and Maxillofacial Surgery, Kyushu Dental University, 2-6-1 Manazuru, Kokurakitaku, Kitakyushu, Fukuoka 803-8580, Japan, Department of Applied Chemistry and Research Center for Bio-microsensing Technology, Kyushu Institute of Technology,1-1 Sensui, Tobataku, Kitakyushu, Fukuoka 804-8550, Japan, Department of Health Promotion, Division of Oral Pathology, Kyushu Dental University, 2-6-1 Manazuru, Kokurakitaku, Kitakyushu, Fukuoka 803-8580, Japan, Department of Science of Physical Functions, Division of Oral Medicine, Kyushu Dental University, 2-6-1 Manazuru, Kokurakitaku, Kitakyushu, Fukuoka 803-8580, Japan, Haraguchi, Kazuya, Sato, Shinobu, Habu, Manabu, Yada, Naomi, Hayakawa, Mana, Takahashi, Osamu, Yoshioka, Izumi, Matsuo, Kou, Tominaga, Kazuhiro, and Takenaka, Shigeori

Abstract

type:Journal Article, Ferrocenylnaphthalene diimide‐based electrochemical hybridization assay via a multi‐electrode chip was applied to detect the methylation frequency in the promoter region of human telomerase reverse transcriptase (hTERT) gene for clinical samples from tissues, local exfoliated oral cells from a lesion, or from entire oral cavity after their methylation specific PCRs. These methylation frequencies were increased with cancer progress as the following order: healthy volunteers, oral leukoplakia as precancerous lesion, and oral squamous cell carcinoma (OSCC). Operating characteristic analysis of the obtained current data doesn't only give excellent discrimination ability of OSCC, but also of oral leukoplakia from healthy volunteers for all samples. Sensitivity and specificity was 95% and 90%, respectively, which is a comparable with methods in practical use.

Published
2019

24. Association between Psychological Distress and Occlusion among Temporomandibular Disorder Patients - A Clinical Study

Author

Lakshmi, S.; Department of Oral medicine and Radiology, The Oxford Dental College, Bangalore, Dyasanoor, S.; Department of Oral medicine and Radiology, The Oxford Dental College, Bangalore, Lakshmi, S.; Department of Oral medicine and Radiology, The Oxford Dental College, Bangalore, and Dyasanoor, S.; Department of Oral medicine and Radiology, The Oxford Dental College, Bangalore

Abstract

Background: TMD is a multifactorial disorder significant research efforts have been invested in finding its etiologies and management to improve their quality of life.Objectives: To evaluate and compare digital occlusal parameters, depression, anxiety and serum cortisol level between temporomandibular disorder patients and control group.Material and Methods: A sample size of 24 TMD patients and 24 apparently healthy age and sex matched controls were recruited in the study. The patients occlusion time and disclusion time was recorded using T-Scan III, the level of anxiety and depression was evaluated by using Hospital Anxiety and Depression scale questtionaire and serum cortisol was evaluated by collecting fasting blood sample, under aseptic conditions.Results: The incidence of premature contacts (occlusal interferences), clusion time and disclusion time was significantly longer in TMD group than in control subjects. The anxiety and depression scores were statistically significant in TMD group compared to control group, but no statistically significant difference was observed in serum cortisol levels.Conclusion: The present study demonstrated increase in the premature contacts i.e. occlusal interferences were high with increase in clusion time, disclusion time, anxiety and depression scores in TMD group, where as no significant increase in serum cortisol levels was seen among TMD group even though their anxiety and depression levels were high. Hence, patients with temporomandibular disorders should always be evaluated for stress and anxiety along with the T-Scan evaluation of occlusal prematurities and correction of the same.

Published
2016

25. Radicular Dens Invaginatus Associated With Radicular Cyst in Maxillary Third Molar - Rare Case Report

Author

Gannepalli, Ashalata; Department of Maxillofacial Pathology and Microbiology, Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre, Hyderabad, Telangana, Ayinampudi, Bhargavi Krishna; Department of Maxillofacial Pathology and Microbiology, Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre, Hyderabad, Telangana, Podduturi, Sanjay Reddy; Department of Oral Medicine and Radiology, Lenora Institute of Dental Sciences, Rajahmundry, Andhra Pradesh, Gannepalli, Ashalata; Department of Maxillofacial Pathology and Microbiology, Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre, Hyderabad, Telangana, Ayinampudi, Bhargavi Krishna; Department of Maxillofacial Pathology and Microbiology, Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre, Hyderabad, Telangana, and Podduturi, Sanjay Reddy; Department of Oral Medicine and Radiology, Lenora Institute of Dental Sciences, Rajahmundry, Andhra Pradesh

Abstract

Dens invaginatus is a developmental tooth anomaly showing broad spectrum of morphological variations. Most extreme form of the invagination results in a bulbous expansion of the affected crown and/or root has been termed as dilated odontome. Radicular dens invaginatus is a rare dentalanomaly formed by infolding ofHertwig's epithelial root sheath and in few forms invagination is lined by enamel. Various techniques of treatment including conservative treatment, nonsurgical root canal treatment, and endodontic surgery usually are unsuccessful because of their complex morphology and are associated with periapical lesions. We report a case of radicular dens in dente with dilated root and was associated with radicular cyst in the right maxillary third molar in a patient aged 38 years. Histological examination showed a dilated bulbous root with enamel formation within root invagination and the associated radicular cyst was lined by pseudostratified ciliated columnar epithelium.

Published
2018

26. Evaluation and Comparative Study of Palatal Rugae, Arch Length, and Arch Width with its Predictive Accuracy for Gender Identification

Author

Patel, Nirali; Intern, Manubhai Patel Dental College & Hospital, Vadodara 390011, Gujarat, India, Joshi, Manjiri; Reader and Head, Department of Oral Medicine and Radiology, Manubhai Patel Dental College & Hospital, Vadodara 390011, Gujarat, Patel, Nirali; Intern, Manubhai Patel Dental College & Hospital, Vadodara 390011, Gujarat, India, and Joshi, Manjiri; Reader and Head, Department of Oral Medicine and Radiology, Manubhai Patel Dental College & Hospital, Vadodara 390011, Gujarat

Abstract

Aim: The aim of this study was to evaluate and correlate variation in the palatal rugae pattern, maxillary arch length, and maxillary arch width in males and females visiting dental college along with its predictive accuracy in gender differentiation. Materials and methods: 100 preorthodontic maxillary dental stone casts, including 50 males and 50 females in age range of 18–30 years, were studied for different rugae pattern, arch length, and arch width. Thomas and Kotze classification was adopted for assessing rugae pattern. The arch length was measured by using the Lavelle's method and arch width by measuring intermolar distance. The data were analyzed using independent t-test and discriminant function analysis by SPSS 19 Statistical Package. Results: The study revealed that females had higher curved and diverging type of rugae while males had higher circular and converging type of rugae, which were found to be statistically significant (P < 0.05). The arch length and width at all dimensions were higher for males than females, which were found to be statistically significant (P < 0.05). Association between rugae pattern and arch dimension was computed using discriminant function analysis, which permitted gender differentiation in this population with an accuracy of 83%. Conclusion: The rugae pattern, arch length, and arch width can be used for gender differentiation in the human population. However, further research work on a larger population and use of other methods is necessary to validate its use in forensics science.

Published
2018

27. Influence of Alveolar Bone Height on Stress Distribution of Endodontically Treated Teeth with Post: A 3D Finite Element Analysis

Author

Singh, S. Vijay; Department of Conservative Dentistry and Endodontics, D.A.V. (C) Dental College, Yamuna Nagar, Haryana 135001, Pandit, I. K.; Department of Paedodontics and Preventive Dentistry, D.A.V. (C) Dental College, Yamuna Nagar, Haryana 135001, Gupta, Saurabh; Department of Conservative Dentistry and Endodontics, D.A.V. (C) Dental College, Yamuna Nagar, Haryana, Singh, Aruna Vijay; Department of Oral Medicine and Radiology, D.A.V. (C) Dental College and Hospital, Yamuna Nagar, Haryana 135001, Satija, Harsha; Department of Conservative Dentistry and Endodontics, D.A.V. (C) Dental College, Yamuna Nagar, Haryana 135001, Singh, S. Vijay; Department of Conservative Dentistry and Endodontics, D.A.V. (C) Dental College, Yamuna Nagar, Haryana 135001, Pandit, I. K.; Department of Paedodontics and Preventive Dentistry, D.A.V. (C) Dental College, Yamuna Nagar, Haryana 135001, Gupta, Saurabh; Department of Conservative Dentistry and Endodontics, D.A.V. (C) Dental College, Yamuna Nagar, Haryana, Singh, Aruna Vijay; Department of Oral Medicine and Radiology, D.A.V. (C) Dental College and Hospital, Yamuna Nagar, Haryana 135001, and Satija, Harsha; Department of Conservative Dentistry and Endodontics, D.A.V. (C) Dental College, Yamuna Nagar, Haryana 135001

Abstract

Objective: Adequate bone support is an essential factor to avoid undue stress to the tooth. This is especially important, when the tooth is endodontically treated and requires a post. The purpose of the present finite element analysis study was to evaluate the stress distribution in endodontically treated teeth with post with different alveolar bone height.Method and materials: A 3D model was fabricated using software to represent an endodontically treated mandibular second premolar restored with titanium post and a full ceramic crown restoration and was analyzed using finite element analysis. A load of 300 N at an angle of 60 to the vertical was applied to the triangular ridge of the buccal cusp in a buccolingual plane. The relationship of alveolar bone height at 2 mm and 4 mm was calculated using von Mises stresses.Results: The finite element analysis showed the maximum stresses of 137.43 Mpa in dentin with alveolar bone height of 4 mm as compared to 105.91 Mpa in the model with alveolar bone height of 2 mm from the CEJ.Conclusion: Stress was observed more in alveolar bone height level of 4 mm from CEJ than 2 mm alveolar bone height level from CEJ.

Published
2018

28. Contributors

Author

Austin, Rupert, Bains, Davinder, Banerjee, Avijit, Bavisha, Kalpesh A., Bellis, Wendy, Bister, Dirk, Bomfim, Deborah, Burke, Mary, Carey, Barbara, Cascarini, Luke, Chauhan, Ravi, Crighton, Alexander, Davies, Rodhri, D’Cruz, Len, Dickinson, Chris, Djemal, Serpil, Drage, Nicholas, Escudier, Michael, Ghuman, Mandeep, Goodger, Nicholas M., Harris, Jennifer C., Harrison, Mike, Heidari, Ellie, Hullah, Esther, Joshi, Sandeep, Lalli, Anand, Mackenzie, Louis, Mannocci, Francesco, McParland, Helen, Nayee, Shalini, O’Neill, Niall, Odell, Edward, Ormond, Martyn, Patel, Vinod, Radford, David R., Renton, Tara, Rooney, Yvonne M., Setterfield, Jane, Sherwin, Emily, Shirlaw, Pepe, Tappuni, Anwar R., Thavaraj, Selvam, Thomas, Bethan, Thomas, Michael, Tilakaratne, Wanninayaka M., Turner, Jonathan, Watkins, Sophie, and Whaites, Eric

Published
2020
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29. Sirtuin-3 (SIRT3), a novel potential therapeutic target for oral cancer

Author

Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan ; Department of Pathology, University of Michigan, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan ; Fax: (734) 763-5503 ; Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, Gladstone Institute of Virology and Immunology, University of California, San Francisco, California, Alhazzazi, Turki Y., Kamarajan, Pachiyappan, Joo, Nam, Huang, Jing-Yi, Verdin, Eric, D'Silva, Nisha J., Kapila, Yvonne L., Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan ; Department of Pathology, University of Michigan, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan ; Fax: (734) 763-5503 ; Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, Gladstone Institute of Virology and Immunology, University of California, San Francisco, California, Alhazzazi, Turki Y., Kamarajan, Pachiyappan, Joo, Nam, Huang, Jing-Yi, Verdin, Eric, D'Silva, Nisha J., and Kapila, Yvonne L.

Abstract

BACKGROUND: Several sirtuin family members (SIRT1-7), which are evolutionarily conserved NAD-dependent deacetylases, play an important role in carcinogenesis. However, their role in oral cancer has not yet been investigated. Therefore, the objective of this study was to investigate whether sirtuins play a role in oral cancer carcinogenesis. METHODS: The expression levels of all sirtuins in several oral squamous cell carcinoma (OSCC) cell lines were compared with normal human oral keratinocytes and observed that SIRT3 was highly expressed. Therefore, tissue microarrays were used to evaluate the clinical relevance of this overexpression. SIRT3 down-regulation in OSCC cell proliferation and survival was investigated and analyzed by using cell-proliferation and cell-viability assays. Ionizing radiation and cisplatin were used to investigate whether SIRT3 down-regulation could increase the sensitivity of OSCC to both treatments. To further assess the in vivo role of SIRT3 in OSCC carcinogenesis, a floor-of-mouth oral cancer murine model was used to study the effect of SIRT3 down-regulation on OSCC tumor growth in immunodeficient mice. RESULTS: The current results demonstrated for the first time that SIRT3 is overexpressed in OSCC in vitro and in vivo compared with other sirtuins. Down-regulation of SIRT3 inhibited OSCC cell growth and proliferation and increased OSCC cell sensitivity to radiation and cisplatin treatments in vitro. SIRT3 down-regulation also reduced tumor burden in vivo. CONCLUSIONS: The current investigation revealed a novel role for SIRT3 in oral cancer carcinogenesis as a promoter of cell proliferation and survival, thus implicating SIRT3 as a new potential therapeutic target to treat oral cancer. Cancer 2011. ?? 2010 American Cancer Society.

Published
2011

30. Parotid Sparing Study in Head and Neck Cancer Patients Receiving Bilateral Radiation Therapy: One-year Results

Author

Department of Oral Medicine, Pathology, Surgery, University of Michigan School of Dentistry, 1011 N. University, Room 2010, Ann Arbor, Michigan 48109-1078, Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan, Department of Oral Medicine, Pathology, Surgery, University of Michigan School of Dentistry, 1011 N. University, Room 2010, Ann Arbor, Michigan 48109-1078, 0dessa Brown Children's Clinic, Seattle, WA, Ship, J. A., Eisbruch, A., D'Hondt, E., Jones, R. E., Department of Oral Medicine, Pathology, Surgery, University of Michigan School of Dentistry, 1011 N. University, Room 2010, Ann Arbor, Michigan 48109-1078, Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan, Department of Oral Medicine, Pathology, Surgery, University of Michigan School of Dentistry, 1011 N. University, Room 2010, Ann Arbor, Michigan 48109-1078, 0dessa Brown Children's Clinic, Seattle, WA, Ship, J. A., Eisbruch, A., D'Hondt, E., and Jones, R. E.

Abstract

Many patients with head and neck cancers receive radiation therapy as part of their treatment which frequently causes considerable morbidity, including various degrees of permanent salivary gland dysfunction. Three-dimensional treatment planning [3-DTP] and conformational dose delivery constitute a new therapeutic modality that conforms the high-dose radiation volume to the shape of the tumor volume while minimizing the dose to tissue that is not at risk of containing cancer. The treatment volumes for head and neck tumors as well as parotid glands can be well-defined on cross-sectional CT imaging techniques. The purpose of this investigation is to determine if 3-DTP and conformational dose-delivery could minimize radiation dose and salivary gland dysfunction to contralateral parotid glands in patients with head and neck cancers. Eleven patients with head and neck cancers who required bilateral radiation therapy were treated with 3-DTP. Unstimulated and stimulated bilateral parotid saliva was collected prior to radiotherapy, weekly during treatment, and 1, 3, 6, and 12 months after the completion of radiotherapy. Treated parotid glands received an average dose of 5745 cGy, while spared glands received only 1986 cGy (p < 0.0001). Unstimulated and stimulated parotid flow rates decreased dramatically in treated glands after the initiation of radiotherapy, remained at extremely low rates without any improvements, and were significantly lower at 1 year after radiotherapy compared with baseline. Conversely, parotid flow rates in spared glands underwent mild changes during radiotherapy and were approximately 50% of baseline values. The results of this study suggest that with the use of 3-DTP, contralateral parotid gland function can be partially preserved for at least 1 year in patients with head and neck cancers requiring bilateral radiation.

Published
2010

31. Differentiation-dependent association of phosphorylated extracellular signal-regulated kinase with the chromatin of osteoblast-related genes

Author

Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry ; Department of Biological Chemistry, University of Michigan School of Medicine, 1011 N. University Ave., Ann Arbor, MI, USA ; Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, 1011 North University Ave., Ann Arbor, MI 48109, USA., Li, Yan, Ge, Chunxi, Franceschi, Renny T., Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry ; Department of Biological Chemistry, University of Michigan School of Medicine, 1011 N. University Ave., Ann Arbor, MI, USA ; Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, 1011 North University Ave., Ann Arbor, MI 48109, USA., Li, Yan, Ge, Chunxi, and Franceschi, Renny T.

Abstract

The ERK/MAP kinase pathway is an important regulator of gene expression and differentiation in postmitotic cells. To understand how this pathway controls gene expression in bone, we examined the subnuclear localization of P-ERK in differentiating osteoblasts. Induction of differentiation was accompanied by increased ERK phosphorylation and expression of osteoblast-related genes, including osteocalcin ( Bglap2 ) and bone sialoprotein ( Ibsp ). Confocal immunofluorescence microscopy revealed that P-ERK colocalized with the RUNX2 transcription factor in the nuclei of differentiating cells. Interestingly, a portion of this nuclear P-ERK was directly bound to the proximal promoter regions of Bglap2 and Ibsp . Furthermore, the level of P-ERK binding to chromatin increased with differentiation, whereas RUNX2 binding remained relatively constant. The P-ERK-chromatin interaction was seen only in RUNX2-positive cells, required intact RUNX2-selective enhancer sequences, and was blocked with MAPK inhibition. These studies show for the first time that RUNX2 specifically targets P-ERK to the chromatin of osteoblast-related genes, where it may phosphorylate multiple substrates, including RUNX2, resulting in altered chromatin structure and gene expression. ?? 2010 American Society for Bone and Mineral Research

Published
2010

32. Tumor expressed PTHrP facilitates prostate cancer-induced osteoblastic lesions

Author

Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI, Department of Urology, Urology Center, University of Michigan, Ann Arbor, MI ; Department of Internal Medicine, University of Michigan, Ann Arbor, MI, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI ; Department of Pathology, Medical School, University of Michigan, Ann Arbor, MI ; Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA, Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus, OH, Liao, Jinhui, Li, Xin, Koh, Amy J., Berry, Janice E., Thudi, Nanda, Rosol, Thomas J., Pienta, Kenneth J., McCauley, Laurie K., Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI, Department of Urology, Urology Center, University of Michigan, Ann Arbor, MI ; Department of Internal Medicine, University of Michigan, Ann Arbor, MI, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI ; Department of Pathology, Medical School, University of Michigan, Ann Arbor, MI ; Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA, Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus, OH, Liao, Jinhui, Li, Xin, Koh, Amy J., Berry, Janice E., Thudi, Nanda, Rosol, Thomas J., Pienta, Kenneth J., and McCauley, Laurie K.

Abstract

Expression of parathyroid hormone-related protein (PTHrP) correlates with prostate cancer skeletal progression; however, the impact of prostate cancer-derived PTHrP on the microenvironment and osteoblastic lesions in skeletal metastasis has not been completely elucidated. In this study, PTHrP overexpressing prostate cancer clones were stably established by transfection of full length rat PTHrP cDNA. Expression and secretion of PTHrP were verified by western blotting and IRMA assay. PTHrP overexpressing prostate cancer cells had higher growth rates in vitro , and generated larger tumors when inoculated subcutaneously into athymic mice. The impact of tumor-derived PTHrP on bone was investigated using a vossicle co-implant model. Histology revealed increased bone mass adjacent to PTHrP overexpressing tumor foci, with increased osteoblastogenesis, osteoclastogenesis and angiogenesis. In vitro analysis demonstrated pro-osteoclastic and pro-osteoblastic effects of PTHrP. PTHrP enhanced proliferation of bone marrow stromal cells and early osteoblast differentiation. PTHrP exerted a pro-angiogenic effect indirectly, as it increased angiogenesis but only in the presence of bone marrow stromal cells. These data suggest PTHrP plays a role in tumorigenesis in prostate cancer, and that PTHrP is a key mediator for communication and interactions between prostate cancer and the bone microenvironment. Prostate cancer-derived PTHrP is actively involved in osteoblastic skeletal progression. ?? 2008 Wiley-Liss, Inc.

Published
2008

33. Annexin II/Annexin II receptor axis regulates adhesion, migration, homing, and growth of prostate cancer

Author

Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan ; Harvard School of Dental Medicine, Boston, Massachusetts, Department of Internal Medicine, Division of Hemotology/Oncology, University of Michigan School of Medicine, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan ; Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Room 3307, 1011 North University Avenue, Ann Arbor, MI 48109-1078., Medicine-Hematology/Oncology, University of Pittsburgh, Pittsburgh, Pennsylvania, Shiozawa, Yusuke, Havens, Aaron M., Jung, Younghun, Ziegler, Anne M., Pedersen, Elisabeth A., Wang, Jingcheng, Wang, Jianhua, Lu, Ganwei, Roodman, G. David, Loberg, Robert D., Pienta, Kenneth J., Taichman, Russell S., Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan ; Harvard School of Dental Medicine, Boston, Massachusetts, Department of Internal Medicine, Division of Hemotology/Oncology, University of Michigan School of Medicine, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan ; Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Room 3307, 1011 North University Avenue, Ann Arbor, MI 48109-1078., Medicine-Hematology/Oncology, University of Pittsburgh, Pittsburgh, Pennsylvania, Shiozawa, Yusuke, Havens, Aaron M., Jung, Younghun, Ziegler, Anne M., Pedersen, Elisabeth A., Wang, Jingcheng, Wang, Jianhua, Lu, Ganwei, Roodman, G. David, Loberg, Robert D., Pienta, Kenneth J., and Taichman, Russell S.

Abstract

One of the most life-threatening complications of prostate cancer is skeletal metastasis. In order to develop treatment for metastasis, it is important to understand its molecular mechanisms. Our work in this field has drawn parallels between hematopoietic stem cell and prostate cancer homing to the marrow. Our recent work demonstrated that annexin II expressed by osteoblasts and endothelial cells plays a critical role in niche selection. In this study, we demonstrate that annexin II and its receptor play a crucial role in establishing metastasis of prostate cancer. Prostate cancer cell lines migrate toward annexin II and the adhesion of prostate cancer to osteoblasts and endothelial cells was inhibited by annexin II. By blocking annexin II or its receptor in animal models, short-term and long-term localization of prostate cancers are limited. Annexin II may also facilitate the growth of prostate cancer in vitro and in vivo by the MAPK pathway. These data strongly suggest that annexin II and its receptor axis plays a central role in prostate cancer metastasis, and that prostate cancer utilize the hematopoietic stem cell homing mechanisms to gain access to the niche. J. Cell. Biochem. 105: 370???380, 2008. ?? 2008 Wiley-Liss, Inc.

Published
2008

34. Bone marrow stromal cells as a genetic platformfor systemic delivery of therapeutic proteins in vivo human factor IX model.

Author

University of Michigan School of Dentistry, Department of Oral Medicine, Pathology, and Oncology, Ann Arbor, Michigan, USA ; University of Michigan School of Dentistry, Dept. of Oral Medicine, Pathology, and Oncology, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA., University of Michigan School of Medicine, Department of Human Genetics, Ann Arbor, Michigan, USA, Krebsbach, Paul H., Zhang, Kezhong, Malik, Ajay K., Kurachi, Kotoku, University of Michigan School of Dentistry, Department of Oral Medicine, Pathology, and Oncology, Ann Arbor, Michigan, USA ; University of Michigan School of Dentistry, Dept. of Oral Medicine, Pathology, and Oncology, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA., University of Michigan School of Medicine, Department of Human Genetics, Ann Arbor, Michigan, USA, Krebsbach, Paul H., Zhang, Kezhong, Malik, Ajay K., and Kurachi, Kotoku

Abstract

Background Hemophilia B is an X-linked bleeding disorder that results from a deficiency in functional coagulation factor IX (hFIX). In patients lacking FIX, the intrinsic coagulation pathway is disrupted leading to a lifelong, debilitating and sometimes fatal disease. Methods We have developed an ex vivo gene therapy system using genetically modified bone marrow stromal cells (BMSCs) as a platform for sustained delivery of therapeutic proteins into the general circulation. This model exploits the ability of BMSCs to form localized ectopic ossicles when transplanted in vivo . BMSCs were transduced with MFG-hFIX, a retroviral construct directing the expression of hFIX. The biological activity of hFIX expressed by these cells was assessed in vitro and in vivo . Results Transduced cells produced biologically active hFIX in vitro with a specific activity of 90% and expressed hFIX at levels of???497 ng/10 6 cells/24 h and 322 ng/10 6 cells/24 h for human and porcine cells, respectively. The secretion of hFIX was confirmed by Western blot analysis of the conditioned medium using a hFIX-specific antibody. Transduced BMSCs (8?? 10 6 cells per animal) were transplanted within scaffolds into subcutaneous sites in immunocompromised mice. At 1 week post-implantation, serum samples contained hFIX at levels greater than 25 ng/ml. Circulating levels of hFIX gradually decreased to 11.5 ng/ml at 1 month post-implantation and declined to a stable level at 6.1 ng/ml at 4 months. Conclusions These findings demonstrate that genetically modified BMSCs can continuously secrete biologically active hFIX from self-contained ectopic ossicles in vivo, and thus represent a novel delivery system for releasing therapeutic proteins into the circulation. Copyright?? 2002 John Wiley & Sons, Ltd.

Published
2006

36. Clinician's Guide to Pharmacology in Dental Medicine

Author

Jacobsen, Peter L., Casiglia, Jeffrey M., American Academy of Oral Medicine, Jacobsen, Peter L., Casiglia, Jeffrey M., and American Academy of Oral Medicine

Subjects
Dental pharmacology
Abstract

This monograph is intended to provide information pertaining to salivary gland and chemosensory disorders. Areas such as xerostomia, salivary gland disfunction, Sjogren's Syndrome, hypersalivation, halitosis, and chemosensory disorders will be covered. The information in this guide will be useful in a private office, as well as in an institutional setting.

Published
2009

37. Clinician's Guide to Diagnosis and Treatment of Chronic Orofacial Pain

Author

Epstein, Joel B., Arm, Robert N., Brown, Ronald S., American Academy of Oral Medicine, Epstein, Joel B., Arm, Robert N., Brown, Ronald S., and American Academy of Oral Medicine

Subjects
Orofacial pain, Chronic pain
Abstract

This monograph is a current and concise review for the clinician providing oral health care for chronic orofacial pain patients. This guide was written for clinicians in a ready-to-use format to serve as a quick reference for many chronic orofacial pain conditions.

Published
2009

38. Clinician's Guide : Pharmacology in Dental Medicine

Author

Jacobsen, Peter L., Casiglia, Jeffrey M., American Academy of Oral Medicine, Jacobsen, Peter L., Casiglia, Jeffrey M., and American Academy of Oral Medicine

Subjects
Dental pharmacology
Abstract

This monograph is intended to provide information pertaining to the use of phamracologic agents that may be used by the dental practitioner in general or specialty practice. The subjects are grouped by either specific classes of therapeutic agent or by pharmacologic concept.

Published
2009

39. Clinician's Guide : Tobacco Cessation

Author

Mohammad, Abdel Rahim, American Academy of Oral Medicine, Mohammad, Abdel Rahim, and American Academy of Oral Medicine

Subjects
Smoking cessation--Handbooks, manuals, etc, Nicotine addiction--Treatment--Handbooks, manuals, etc
Abstract

This monograph is intended as a concise handbook which offers a useful outline of common oral diseases and dental conditions found in smokers and alerts dental professionals to the systemic diseases hazarded by tobacco users.

Published
2009

40. Effect of erlotinib on epidermal growth factor receptor and downstream signaling in oral cavity squamous cell carcinoma

Author

Internal Medicine, Division of Hematology???Oncology, University of Michigan Medical Center, Ann Arbor, Michigan, Department of Oral Medicine/Pathology and Oncology, School of Dentistry, University of Michigan, Ann Arbor, Michigan, University of Michigan Medical Center, Department of Radiation Oncology, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan, Department of Otolaryngology, University of Michigan Medical Center, Ann Arbor, Michigan, Oral and Maxillofacial Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, Tsien, Christina I., Nyati, Mukesh K., Ahsan, Aarif, Ramanand, Susmita G., Chepeha, Douglas B., Worden, Francis P., Helman, Joseph I., D'Silva, Nisha, Bradford, Carol R., Wolf, Gregory T., Lawrence, Theodore S., Eisbruch, Avraham, Internal Medicine, Division of Hematology???Oncology, University of Michigan Medical Center, Ann Arbor, Michigan, Department of Oral Medicine/Pathology and Oncology, School of Dentistry, University of Michigan, Ann Arbor, Michigan, University of Michigan Medical Center, Department of Radiation Oncology, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan, Department of Otolaryngology, University of Michigan Medical Center, Ann Arbor, Michigan, Oral and Maxillofacial Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, Tsien, Christina I., Nyati, Mukesh K., Ahsan, Aarif, Ramanand, Susmita G., Chepeha, Douglas B., Worden, Francis P., Helman, Joseph I., D'Silva, Nisha, Bradford, Carol R., Wolf, Gregory T., Lawrence, Theodore S., and Eisbruch, Avraham

Abstract

Background The purpose of this study was to determine if there are differences in biomarker modulation and epidermal growth factor receptor (EGFR) degradation between the tumor and the normal mucosa after treatment with an EGFR inhibitor, erlotinib, in head and neck cancer. Methods Patients with primary oral cavity squamous cell cancers received a course of erlotinib, 150 mg every day for 7 days before surgical resection. Tumor and normal mucosa biopsies were obtained both pre???erlotinib and post???erlotinib. Changes in known markers of EGFR activity (phospho, AKT, STAT3) were measured by immunoblotting, whereas changes in tissue distribution were analyzed by immunohistochemical analysis. Results Twelve patients were enrolled; 7 had evaluable paired tumors and normal mucosa biopsies pretreatment and posttreatment. Expression of EGFR was higher in tumors compared to the normal mucosa ( p = .005). Erlotinib administration was associated with marked inhibition of phosphorylated epidermal growth factor receptor (pEGFR) and reduction in total EGFR protein ( p = .004, p = .007) in tumors, whereas there was heterogeneity in EGFR inhibition in the normal mucosa ( p = .10 [pEGFR], and p = .07 [EGFR]). Reduced levels of pSrc and pSTAT3 and enhanced p27 levels were noted in tumors after erlotinib. Cell culture studies confirmed that EGFR is degraded in tumor cells after prolonged treatment with erlotinib. Conclusion Our results show that EGFR inhibition by erlotinib led to a marked reduction in EGFR protein levels in patients. Differential effects of erlotinib on tumors compared to the normal mucosa suggest there may be individual patient heterogeneity. These preliminary data suggest EGFR degradation should be further analyzed as a potential biomarker in selecting patients likely to benefit from EGFR inhibitors. ?? 2012 Wiley Periodicals, Inc. Head Neck, 2013

Published
2013

41. Dickkopf-1 (DKK-1) stimulated prostate cancer growth and metastasis and inhibited bone formation in osteoblastic bone metastases

Author

Departments of Urology and Pathology, School of Medicine, University of Michigan, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan, M.D. Anderson Cancer Center, University of Texas, Houston, Texas, Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, Ohio, Department of Internal Medicine, The Ohio State University, Columbus, Ohio, Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio ; 1925 Coffey Road, Columbus, OH 43210., Thudi, Nanda K., Martin, Chelsea K., Murahari, Sridhar, Shu, Sherry T., Lanigan, Lisa G., Werbeck, Jillian L., Keller, Evan T., McCauley, Laurie K., Pinzone, Joseph J., Rosol, Thomas J., Departments of Urology and Pathology, School of Medicine, University of Michigan, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Michigan, M.D. Anderson Cancer Center, University of Texas, Houston, Texas, Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, Ohio, Department of Internal Medicine, The Ohio State University, Columbus, Ohio, Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio ; 1925 Coffey Road, Columbus, OH 43210., Thudi, Nanda K., Martin, Chelsea K., Murahari, Sridhar, Shu, Sherry T., Lanigan, Lisa G., Werbeck, Jillian L., Keller, Evan T., McCauley, Laurie K., Pinzone, Joseph J., and Rosol, Thomas J.

Abstract

BACKGROUND Osteoblastic bone metastasis is the predominant phenotype observed in prostate cancer patients and is associated with high patient mortality and morbidity. However, the mechanisms determining the development of this phenotype are not well understood. Prostate cancer cells secrete several osteogenic factors including Wnt proteins, which are not only osteoinductive but also oncogenic. Therefore, the purpose of the study was to investigate the contribution of the Wnt signaling pathway in prostate cancer growth, incidence of bone metastases, and osteoblastic phenotype of bone metastases. The strategy involved overexpressing the Wnt antagonist, DKK-1, in the mixed osteoblastic and osteolytic Ace-1 prostate cancer cells. METHODS Ace-1 prostate cancer cells stably expressing human DKK-1 or empty vector were established and transduced with lentiviral yellow fluorescent protein (YFP)-luciferase (Luc). The Ace-1/vector YFP-LUC and Ace-1/DKK-1 YFP-LUC cells were injected subcutaneously, intratibially, or in the left cardiac ventricle in athymic mice. RESULTS Unexpectedly, DKK-1 significantly increased Ace-1 subcutaneous tumor mass and the incidence of bone metastases after intracardiac injection of Ace-1 cells. DKK-1 increased Ace-1 tumor growth associated with increased phospho46 c-Jun amino-terminal kinase by the Wnt noncanonical pathway. As expected, DKK-1 decreased the Ace-1 osteoblastic phenotype of bone metastases, as confirmed by radiographic, histopathologic, and microcomputed tomographic analysis. DKK-1 decreased osteoblastic activity via the Wnt canonical pathway evidenced by an inhibition of T-cell factor activity in murine osteoblast precursor ST2 cells. CONCLUSION The present study showed that DKK-1 is a potent inhibitor of bone growth in prostate cancer-induced osteoblastic metastases. Prostate 71:615???625, 2011. ?? 2010 Wiley-Liss, Inc.

Published
2011

42. HPV-positive/p16-positive/EBV-negative nasopharyngeal carcinoma in white North Americans

Author

University of Michigan Medical School, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Department of Otolaryngology/Head and Neck Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, Departments of Otolaryngology???Head and Neck Surgery, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Department of Otolaryngology/Head and Neck Surgery, Ohio State University, Columbus, OH, Radiation Oncology, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Pathology, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Pathology, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Periodontics and Oral Medicine, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Internal Medicine, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Departments of Otolaryngology???Head and Neck Surgery, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Departments of Otolaryngology???Head and Neck Surgery, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Pharmacology, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Departments of Otolaryngology-Head and Neck Surgery, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Department of Otolaryngology/Head and Neck Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, SensiGen, LLC, Ann Arbor, MI; Sequenom, Inc, San Diego, CA, Maxwell, Jessica H., Kumar, Bhavna, Feng, Felix Y., McHugh, Jonathan B., Cordell, Kitrina G., Eisbruch, Avraham, Worden, Francis P., Wolf, Gregory T., Prince, Mark E., Moyer, Jeffrey S., Teknos, Theodoros N., Chepeha, Douglas B., Stoerker, Jay, Walline, Heather, Carey, Thomas E., Bradford, Carol R., University of Michigan Medical School, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Department of Otolaryngology/Head and Neck Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, Departments of Otolaryngology???Head and Neck Surgery, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Department of Otolaryngology/Head and Neck Surgery, Ohio State University, Columbus, OH, Radiation Oncology, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Pathology, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Pathology, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Periodontics and Oral Medicine, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Internal Medicine, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Departments of Otolaryngology???Head and Neck Surgery, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Departments of Otolaryngology???Head and Neck Surgery, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Pharmacology, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI ; Departments of Otolaryngology-Head and Neck Surgery, The University of Michigan Health System and Comprehensive Cancer Center, Ann Arbor, MI, Department of Otolaryngology/Head and Neck Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, SensiGen, LLC, Ann Arbor, MI; Sequenom, Inc, San Diego, CA, Maxwell, Jessica H., Kumar, Bhavna, Feng, Felix Y., McHugh, Jonathan B., Cordell, Kitrina G., Eisbruch, Avraham, Worden, Francis P., Wolf, Gregory T., Prince, Mark E., Moyer, Jeffrey S., Teknos, Theodoros N., Chepeha, Douglas B., Stoerker, Jay, Walline, Heather, Carey, Thomas E., and Bradford, Carol R.

Abstract

Background Human papillomavirus (HPV) has been detected in keratinizing nasopharyngeal carcinomas (NPCs); however, the relationship between HPV and Epstein???Barr virus (EBV) among whites with nonkeratinizing NPCs remains unclear. The HPV, p16, and EBV status was examined in current University of Michigan patients with NPC. Methods From 2003 to 2007, 89 patients, 84 with oropharyngeal cancer (OPC) and 5 with NPC, were enrolled in an organ-sparing trial. Biopsy tissues from all 89 patients were evaluated for HPV and p16 expression. A separate HPV analysis of the 84 OPC patients is in progress. Among the patients with NPC, tumor tissue was also analyzed for EBV-encoded RNA (EBER). Results Five of 89 patients (5.6%) had NPC, all with nonkeratinizing histology. The 4 white patients with NPC were HPV(+) (subtype-16, subtype-18 [2 patients], and subtype-59)/p16(+)/EBER(-). One Asian patient with NPC had an HPV(-)/p16(-)/EBER(+) NPC tumor that developed distant metastases. Conclusion We postulate that HPV may be the etiologic factor in some EBV-negative, nonkeratinizing NPCs among whites. ?? 2009 Wiley Periodicals, Inc. Head Neck, 2010

Published
2010

43. Cytotoxicity and Dentin Permeability of Carbamide Peroxide and Hydrogen Peroxide Vital Bleaching Materials, in vitro

Author

Department of Prosthodontics, The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078, Departments of Oral Medicine, Pathology and Surgery, Department of Cariology and General Dentistry, Hanks, C. T., Fat, J. C., Wataha, J. C., Corcoran, J. F., Department of Prosthodontics, The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078, Departments of Oral Medicine, Pathology and Surgery, Department of Cariology and General Dentistry, Hanks, C. T., Fat, J. C., Wataha, J. C., and Corcoran, J. F.

Abstract

There has been recent concern about the inadvertent exposure of dentin with patent tubules as well as gingiva to bleaching systems containing 10-15% carbamide peroxide or 2-10% hydrogen peroxide for more than a few minutes. The aims of the present study were: (1) to determine the cytotoxicity of dilutions of hydrogen peroxide in cell culture; (2) to measure hydrogen peroxide diffusion from bleaching agents through dentin in vitro; and (3) to determine the risk of hydrogen peroxide-induced cytotoxicity from exposure of dentin to these vital bleaching agents. The 50% inhibitory dose (ID50) of hydrogen peroxide to succinyl dehydrogenase activity in cultured cells was found to be 0.58 mmol/L after 1 h. All bleaching materials demonstrated diffusion of hydrogen peroxide through dentin in an "in vitro pulp chamber" device. The one- and six-hour diffusates of all bleaching agents through 0.5-mm dentin exceeded the ID50 in monolayer cultures. Inhibition of succinyl dehydrogenase activity corresponded to the amount of hydrogen peroxide that can rapidly diffuse through dentin in vitro and reach concentrations which are toxic to cultured cells in less than 1 h.

Published
2010

44. Cytotoxic Effects of Resin Components on Cultured Mammalian Fibroblasts

Author

Departments of Oral Medicine, Pathology and Surgery, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, Biological and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, Hanks, C. T., Strawn, S. E., Watahai, J. C., Craig, R. G., Departments of Oral Medicine, Pathology and Surgery, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, Biological and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, Hanks, C. T., Strawn, S. E., Watahai, J. C., and Craig, R. G.

Abstract

The objectives of this study were to determine the cytotoxic concentrations of 11 components of resin composites on monolayers of cultured Balb/c 3T3 fibroblasts, to study the inhibitory effects of these components on DNA synthesis, total protein content, and protein synthesis, and to determine whether effects were reversible when the components were withdrawn from the medium. These data were reported as concentrations which inhibited 10% (ID10) and 50% (ID50) of a particular metabolic process as well as the range of concentrations over which cell metabolism was irreversibly inhibited. For any individual component, the ID50 values for all three metabolic parameters were of the same magnitude. The same was true for the ranges of irreversibility. Ethoxylated Bis-phenol A dimethacrylate (E-BPA) was the most toxic molecule of the group (ID50 being between 1 and 10 μmol/L). The ID50 concentrations for three of the components, including Bis-GMA, UDMA, TEGDMA, and Bis-phenol A, ranged between 10 and 100 μmol/L, while the ID 50 values of three components (N,N dihydroxyethyl-p-toluidine, camphoroquinone, and N,N dimethylaminoethyl methacrylate) were above 100 μmol/L. The concentrations to which the cells and tissues are exposed in uiuo are not known. This study should help to identify the concentrations of organic composite components which pose clinical cytotoxic hazards.

Published
2010

45. Systematic comparison of the human saliva and plasma proteomes

Author

Departments of Medicine and Genetics and Center for Computational Medicine and Biology, University of Michigan, Ann Arbor, MI, USA, Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, USA, EMBL Outstation, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK, Calibrant Biosystems, Gaithersburg, MD, USA, Biomarkers and Systems Biology Center, Research Triangle Institute, Research Triangle, NC, USA, UCLA School of Dentistry and UCLA Dental Research Institute, University of California-Los Angeles, Los Angeles, CA, USA, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA, Center for Oral Biology, University of Rochester Medical Center, Rochester, NY, USA, Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA, College of Dentistry, New York University, New York, NY, USA, Division of Pulmonary and Critical Care Medicine, University of Southern California, Los Angeles, CA, USA, Department of Oral Medicine, Diagnosis, and Radiology, School of Dentistry, University of Minnesota, Minneapolis, MN, USA, Scripps Research Institute, La Jolla, CA, USA, Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, USA ; Department of Biological Chemistry, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, USA ; Additional corresponding author, UCLA School of Dentistry and UCLA Dental Research Institute, University of California-Los Angeles, Los Angeles, CA, USA ; University of California-Los Angeles, School of Dentistry, 73-017 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA Fax: +1-310-825-7609, Yan, Weihong, Apweiler, Rolf, Balgley, Brian M., Boontheung, Pinmanee, Bundy, Jonathan L., Cargile, Benjamin J., Cole, Steve, Fang, Xueping, Gonzalez-Begne, Mireya, Griffin, Timothy J., Hagen, Fred, Hu, Shen, Wolinsky, Lawrence E., Lee, Cheng S., Malamud, Daniel, Melvin, James E., Menon, Rajasree, Mueller, Michael, Qiao, Renli, Rhodus, Nelson L., Sevinsky, Joel R., States, David, Stephenson, James L., Than, Shawn, Yates, John R., Yu, Weixia, Xie, Hongwei, Xie, Yongming, Omenn, Gilbert S., Loo, Joseph A., Wong, David T., Departments of Medicine and Genetics and Center for Computational Medicine and Biology, University of Michigan, Ann Arbor, MI, USA, Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, USA, EMBL Outstation, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK, Calibrant Biosystems, Gaithersburg, MD, USA, Biomarkers and Systems Biology Center, Research Triangle Institute, Research Triangle, NC, USA, UCLA School of Dentistry and UCLA Dental Research Institute, University of California-Los Angeles, Los Angeles, CA, USA, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA, Center for Oral Biology, University of Rochester Medical Center, Rochester, NY, USA, Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA, College of Dentistry, New York University, New York, NY, USA, Division of Pulmonary and Critical Care Medicine, University of Southern California, Los Angeles, CA, USA, Department of Oral Medicine, Diagnosis, and Radiology, School of Dentistry, University of Minnesota, Minneapolis, MN, USA, Scripps Research Institute, La Jolla, CA, USA, Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, USA ; Department of Biological Chemistry, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, USA ; Additional corresponding author, UCLA School of Dentistry and UCLA Dental Research Institute, University of California-Los Angeles, Los Angeles, CA, USA ; University of California-Los Angeles, School of Dentistry, 73-017 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA Fax: +1-310-825-7609, Yan, Weihong, Apweiler, Rolf, Balgley, Brian M., Boontheung, Pinmanee, Bundy, Jonathan L., Cargile, Benjamin J., Cole, Steve, Fang, Xueping, Gonzalez-Begne, Mireya, Griffin, Timothy J., Hagen, Fred, Hu, Shen, Wolinsky, Lawrence E., Lee, Cheng S., Malamud, Daniel, Melvin, James E., Menon, Rajasree, Mueller, Michael, Qiao, Renli, Rhodus, Nelson L., Sevinsky, Joel R., States, David, Stephenson, James L., Than, Shawn, Yates, John R., Yu, Weixia, Xie, Hongwei, Xie, Yongming, Omenn, Gilbert S., Loo, Joseph A., and Wong, David T.

Abstract

The proteome of human salivary fluid has the potential to open new doors for disease biomarker discovery. A recent study to comprehensively identify and catalog the human ductal salivary proteome led to the compilation of 1166 proteins. The protein complexity of both saliva and plasma is large, suggesting that a comparison of these two proteomes will provide valuable insight into their physiological significance and an understanding of the unique and overlapping disease diagnostic potential that each fluid provides. To create a more comprehensive catalog of human salivary proteins, we have first compiled an extensive list of proteins from whole saliva (WS) identified through MS experiments. The WS list is thereafter combined with the proteins identified from the ductal parotid, and submandibular and sublingual (parotid/SMSL) salivas. In parallel, a core dataset of the human plasma proteome with 3020 protein identifications was recently released. A total of 1939 nonredundant salivary proteins were compiled from a total of 19 14474 unique peptide sequences identified from whole and ductal salivas[semi] 740 out of the total 1939 salivary proteins were identified in both whole and ductal saliva. A total of 597 of the salivary proteins have been observed in plasma. Gene ontology (GO) analysis showed similarities in the distributions of the saliva and plasma proteomes with regard to cellular localization, biological processes, and molecular function, but revealed differences which may be related to the different physiological functions of saliva and plasma. The comprehensive catalog of the salivary proteome and its comparison to the plasma proteome provides insights useful for future study, such as exploration of potential biomarkers for disease diagnostics.

Published
2009

46. Fungiform papilla pattern: EGF regulates inter-papilla lingual epithelium and decreases papilla number by means of PI3K/Akt, MEK/ERK, and p38 MAPK signaling

Author

Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan, Oral Health Sciences Ph.D. Program, School of Dentistry, University of Michigan, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, School of Dentistry, and Department of Pathology, Medical School, University of Michigan, Ann Arbor, Michigan, Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan ; Room 6217, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, Liu, Hong-Xiang, Henson, Bradley S., Zhou, Yanqiu, D'Silva, Nisha J., Mistretta, Charlotte M., Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan, Oral Health Sciences Ph.D. Program, School of Dentistry, University of Michigan, Ann Arbor, Michigan, Department of Periodontics and Oral Medicine, School of Dentistry, and Department of Pathology, Medical School, University of Michigan, Ann Arbor, Michigan, Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan ; Room 6217, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, Liu, Hong-Xiang, Henson, Bradley S., Zhou, Yanqiu, D'Silva, Nisha J., and Mistretta, Charlotte M.

Abstract

Fungiform papillae are epithelial taste organs that form on the tongue, requiring differentiation of papillae and inter-papilla epithelium. We tested roles of epidermal growth factor (EGF) and the receptor EGFR in papilla development. Developmentally, EGF was localized within and between papillae whereas EGFR was progressively restricted to inter-papilla epithelium. In tongue cultures, EGF decreased papillae and increased cell proliferation in inter-papilla epithelium in a concentration-dependent manner, whereas EGFR inhibitor increased and fused papillae. EGF preincubation could over-ride disruption of Shh signaling that ordinarily would effect a doubling of fungiform papillae. With EGF-induced activation of EGFR, we demonstrated phosphorylation in PI3K/Akt, MEK/ERK, and p38 MAPK pathways; with pathway inhibitors (LY294002, U0126, SB203580) the EGF-mediated decrease in papillae was reversed, and synergistic actions were shown. Thus, EGF/EGFR signaling by means of PI3K/Akt, MEK/ERK, and p38 MAPK contributes to epithelial cell proliferation between papillae; this biases against papilla differentiation and reduces numbers of papillae. Developmental Dynamics 237:2378–2393, 2008. © 2008 Wiley-Liss, Inc.

Published
2008

47. Angiogenesis induced by tumor necrosis factor-agr; is mediated by??4 integrins

Author

Department of Oral Medicine, Pathology and Surgery, The University of Michigan, Ann Arbor, MI, USA, Department of Immunology, Texas Biotechnology Corporation, Houston, TX, USA, Department of Cell Biology, Texas Biotechnology Corporation, Houston, TX, USA, Department of Pharmacology, Texas Biotechnology Corporation, Houston, TX, USA, Ann Arbor, Vanderslice, Peter, Munsch, Christy L., Rachal, Eugene, Erichsen, David, Sughrue, Kay M., Truong, Ann N., Wygant, James N., McIntyre, Bradley W., Eskin, Suzanne G., Tilton, Ronald G., Polverini, Peter J., Department of Oral Medicine, Pathology and Surgery, The University of Michigan, Ann Arbor, MI, USA, Department of Immunology, Texas Biotechnology Corporation, Houston, TX, USA, Department of Cell Biology, Texas Biotechnology Corporation, Houston, TX, USA, Department of Pharmacology, Texas Biotechnology Corporation, Houston, TX, USA, Ann Arbor, Vanderslice, Peter, Munsch, Christy L., Rachal, Eugene, Erichsen, David, Sughrue, Kay M., Truong, Ann N., Wygant, James N., McIntyre, Bradley W., Eskin, Suzanne G., Tilton, Ronald G., and Polverini, Peter J.

Abstract

Tumor necrosis factor-?? (TNF-??) and fibroblast growth factor-2 (FGF-2 or bFGF) are potent stimulators of angiogenesis. TNF-??, but not FGF-2, can induce the expression of vascular cell adhesion molecule-1 (VCAM-1) on the surface of endothelial cells. The soluble form of VCAM-1 has recently been demonstrated to function as an angiogenic mediator. Here we demonstrate that monoclonal antibodies directed against VCAM-1 or its??4 integrin counter-receptor inhibited TNF-??-induced endothelial cell migration in vitro. Angiogenesis induced in vivo in rat corneas by TNF-?? was inhibited by a neutralizing antibody directed against the rat??4 integrin subunit. A peptide antagonist of the a4 integrins blocked TNF-??-induced endothelial cell migration in vitro and angiogenesis in rat corneas in vivo. No inhibition by the antibodies or peptide antagonist was observed either in vitro or in vivo when FGF-2 was used as the stimulus. The peptide antagonist did not inhibit TNF-a binding to its receptor nor did it block the function of??v??3, an integrin previously implicated in TNF-a and FGF-2 mediated angiogenesis. These results demonstrate that angiogenic processes induced by TNF-?? are mediated in part by agr;4 integrins possibly by a mechanism involving the induction of soluble VCAM-1.

Published
2006

48. Effect of cell line on in vitro metal ion cytotoxicity

Author

Department of Prosthodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA, Department of Oral Medicine, Pathology and Surgery, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA, Wataha, John C., Hanks, Carl T., Sun, Zhi Lin, Department of Prosthodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA, Department of Oral Medicine, Pathology and Surgery, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA, Wataha, John C., Hanks, Carl T., and Sun, Zhi Lin

Abstract

Objectives. The choice of cell line for in vitro biological tests which assess the cytotoxicity of dental materials remains controversial, yet this issue is important because these tests are widely used to rate the biocompatibility of new and existing materials, and many different cell lines are commonly used. The Purpose of the current study was to quantify the responses of four cell lines (Balb/c 3T3, L929, ROS 17/2.8 and WI-38) to 14 metal ions which are released from dental materials, and relate these responses to the metabolic activity and population doubling times of these cells. Methods. Succinic dehydrogenase (SDH) activity was used to monitor metabolic activity and cytotoxic response. Results. The cell lines responded differently to most metal ions. In general, the Balb/c 3T3 line was the most sensitive, and the WI-38 line was the least sensitive. However, there were many exceptions depending on the metal ion. The passage number of the cells also affected the cytotoxic response. It was concluded that the cytotoxicity of materials which release metal ions will be significantly different depending on which cell line is selected and its passage number. Significance. Based on the findings that cell lines ranked the toxicities of the metal ions similarly, it seems reasonable to use these types of in vitro tests to rank the cytotoxicities of materials. However, if these types of tests are used to predict in vivo cytotoxicity, care should be taken to choose conditions and cells which are relevant.

Published
2006

49. Autoradiographic study of the effects of pulsed electromagnetic fields on bone and cartilage growth in juvenile rats

Author

Department of Orthodontics and Pediatrie Dentistry, Schools of Dentistry and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Department of Cariology and General Dentistry and Biological and Materials Sciences, Schools of Dentistry and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Department of Oral Medicine, Pathology and Surgery, Schools of Dentistry and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Department of Internal Medicine-Nuclear Medicine, Schools of Dentistry and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Wilmot, Janice J., Chiego, Jr, D.J., Carlson, David S., Hanks, C.T., Moskwa, James J., Department of Orthodontics and Pediatrie Dentistry, Schools of Dentistry and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Department of Cariology and General Dentistry and Biological and Materials Sciences, Schools of Dentistry and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Department of Oral Medicine, Pathology and Surgery, Schools of Dentistry and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Department of Internal Medicine-Nuclear Medicine, Schools of Dentistry and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Wilmot, Janice J., Chiego, Jr, D.J., Carlson, David S., Hanks, C.T., and Moskwa, James J.

Abstract

Application of pulsed electromagnetic fields (PEMF) has been used in growth and repair of non-union bone fractures. The similarities between the fibrocartilage callus in non-union bone fractures and the secondary cartilage in the mandibular condyle, both histologically and functionally, lead naturally to study the effects of PEMFs on growth in the condyle. The purposes of this study were: (1) to describe the effects of PEMFs on the growth of the condyle using autoradiography, [3H]-proline and [3H]-thymidine, and (2) to differentiate between the effects of the magnetic and electrical components of the field. Male pre-adolescent Sprague-Dawley rats (28 days old) were divided into three experimental groups of five animals each: (1) PEMF-magnetic (M), (2) PEMF-electrical (E) and (3) control, and were examined at three different times--3, 7 and 14 days of exposure. Each animal was exposed to the field for 8 h per day. Histological coronal sections were processed for quantitative autoradiography to determine the mitotic activity of the condylar cartilage and the amount of bone deposition. The PEMF (magnetic or electrical) had statistically significant effects only on the thickness of the articular zone, with the thickness in the PEMF-M group being the most reduced. Length of treatment was associated with predictable significant changes in the thickness of the condylar cartilage zones and the amount of bone deposition. As the animals aged, less condylar growth was observed and generally the highest mean thicknesses were found in the 3- and 7-day groups; likewise as more bone was deposited, the 7-day measurements of proline-band thickness were significantly greater than the 3-day measurements. Thus, the PEMF-M had a negative effect on the chondrogenic layer of the articular zone, but no other chondrogenic or osteogenic effects were noted.

Published
2006

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