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39 results on '"Orłowska, Blanka"'

2. Comparison of automatic methods MALDI-TOF, VITEK2 and manual methods for the identification of intestinal microbial communities on the example of samples from alpacas (Vicugna pacos)

Author

Pławińska-Czarnak Joanna, Wódz Karolina, Strzałkowska Zuzanna, Żychska Monika, Nowak Tomasz, Kwieciński Adam, Kwieciński Piotr, Bielecki Wojciech, Rodo Anna, Rzewuska Magdalena, Kłosińska Daria, Anusz Krzysztof, and Orłowska Blanka

Subjects
maldi-tof ms, vicugna pacos, intestinal microbiota, opportunistic pathogens, microbial identification, Veterinary medicine, SF600-1100
Abstract

Universally, in microbiological diagnostics the detection of live bacteria is essential. Rapid identification of pathogens enables appropriate remedial measures to be taken. The identification of many bacteria simultaneously facilitates the determination of the characteristics of the accompanying microbiota and/or the microbiological complexity of a given environment.

Published
2023
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3. The eradication of M. caprae tuberculosis in wild boar (Sus scrofa) in the Bieszczady Mountains, southern Poland – an administrative perspective

Author

Welz Mirosław, Krajewska-Wędzina Monika, Orłowska Blanka, Didkowska Anna, Radulski Łukasz, Łoś Przemysław, Weiner Marcin, and Anusz Krzysztof

Subjects
mycobacterium caprae, spoligotype sb2391, tuberculosis, veterinary administration, wild boar, Veterinary medicine, SF600-1100
Abstract

Animal tuberculosis (TB) is a zoonotic disease caused by acid-fast bacteria belonging to the Mycobacterium tuberculosis complex (MTBC). Both animals and humans are susceptible to infection by the MTBC. Interspecies transmission is also possible, including to livestock and humans. In the years 1997–2013, many tuberculosis cases were recorded in European bison in the Bieszczady Mountains; more alarmingly, TB was also recorded in wild boar in the years 2013–2020.

Published
2023
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4. Two centrifugal flotation techniques for counting gastrointestinal parasite eggs and oocysts in alpaca faeces

Author

Maria Pyziel-Serafin Anna, Raboszuk Adrianna, Klich Daniel, Orłowska Blanka, Sierociuk Dagmara, and Anusz Krzysztof

Subjects
faecal examination, flotation techniques, parasites, alpaca, monitoring, Veterinary medicine, SF600-1100
Abstract

The alpaca (Vicugna pacos) is a camelid native to South America, but the species has wide distribution outside its natural habitat and is found in various countries on other continents, Poland being one in Europe. Parasitic infections affect the productivity and health of alpacas. The aim of the study was to estimate the parasite loads in alpacas kept in Poland with the use of two direct centrifugal flotation methods.

Published
2022
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8. Mycobacterial Interspersed Repeat Unit–Variable Number Tandem Repeat Typing of Mycobacterium avium Strains Isolated from the Lymph Nodes of Free-Living Carnivorous Animals in Poland

Author

Orłowska, Blanka, primary, Majchrzak, Marta, additional, Didkowska, Anna, additional, Anusz, Krzysztof, additional, Krajewska-Wędzina, Monika, additional, Zabost, Anna, additional, Brzezińska, Sywia, additional, Kozińska, Monika, additional, Augustynowicz-Kopeć, Ewa, additional, Urbańska, Kaja, additional, Welz, Mirosław, additional, and Parniewski, Paweł, additional

Published
2023
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11. Ten Years of Animal Tuberculosis Monitoring in Free-Living European Bison (Bison bonasus) in Poland

Author

Krajewska-Wędzina, Monika, primary, Krzysiak, Michał K., additional, Bruczyńska, Małgorzata, additional, Orłowska, Blanka, additional, Didkowska, Anna, additional, Radulski, Łukasz, additional, Wiśniewski, Jan, additional, Olech, Wanda, additional, Nowakiewicz, Aneta, additional, Welz, Mirosław, additional, Kaczor, Stanisław, additional, Weiner, Marcin, additional, and Anusz, Krzysztof, additional

Published
2023
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22. Microbiological and molecular monitoring for bovine tuberculosis in the Polish population of European bison (Bison bonasus)

Author

Didkowska, Anna, primary, Orłowska, Blanka, additional, Krajewska-Wędzina, Monika, additional, Augustynowicz-Kopeć, Ewa, additional, Brzezińska, Sylwia, additional, Żygowska, Marta, additional, Wiśniewski, Jan, additional, Kaczor, Stanisław, additional, Welz, Mirosław, additional, Olech, Wanda, additional, and Anusz, Krzysztof, additional

Published
2021
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25. Detection of Mycobacterium tuberculosis Complex Genetic Material in a Free-Living Brown Bear (Ursus arctos).

Author

Orłowska, Blanka, Didkowska, Anna, Brzezińska, Sylwia, Augustynowicz-Kopeć, Ewa, Zabiega, Katarzyna, Pasiniewicz, Marek, Bojarska, Katarzyna, Kaczor, Stanisław, Krajewska-W edzina, Monika, Welz, Mirosław, and Anusz, Krzysztof

Abstract

Mycobacterium tuberculosis complex (MTBC) has rarely been detected in bears (Ursidae). We describe detection of MTBC genetic material using a single-tube, high-multiplex PCR and fluorescence-based detection system in a throat swab collected from a free-living, problem individual during immobilization and telemetry collar deployment. Mycobacterial culture was negative in all samples. [ABSTRACT FROM AUTHOR]

Published
2023
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28. Biopsy and Tracheobronchial Aspirates as Additional Tools for the Diagnosis of Bovine Tuberculosis in Living European Bison (Bison bonasus)

Author

Didkowska, Anna, primary, Orłowska, Blanka, additional, Witkowski, Lucjan, additional, Olbrych, Katarzyna, additional, Brzezińska, Sylwia, additional, Augustynowicz-Kopeć, Ewa, additional, Krajewska-Wędzina, Monika, additional, Bereznowski, Andrzej, additional, Bielecki, Wojciech, additional, Krzysiak, Michał, additional, Rakowska, Alicja, additional, Olech, Wanda, additional, Miller, Michele A., additional, Waters, Wade R., additional, Lyashchenko, Konstantin P., additional, and Anusz, Krzysztof, additional

Published
2020
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29. Pregnancy in European bison (Bison bonaus) with generalized tuberculosis - no evidence of vertical transmission.

Author

Didkowska, Anna, Orłowska, Blanka, Krajewska-Wędzina, Monika, Bielecki, Wojciech, Brzezińska, Sylwia, Augustynowicz-Kopeć, Ewa, Olech, Wanda, and Anusz, Krzysztof

Abstract

Introduction and objective. One of the main health threats to the endangered European bison (Bison bonsasus) is bovine tuberculosis, the pathogenesis of which in this species is not fully known. The aim of the study was to confirm a possible case of vertical transmission from a pregnant European bison with generalized tuberculosis to its 12-week-old foetus. Materials and method. During the autopsy it was found that the bison had become pregnant, despite an advanced stage of tuberculosis. Material collected from the organs and foetus was placed on Lowenstein and Stonebrink media and incubated at 37 °C for 12 weeks. Results. Mycobacteria were isolated from the lungs and lymph nodes; however, the tissue of the foetus and fragments of the reproductive system were negative. Conclusion. Vertical transmission was excluded, although it cannot be ruled out that infection could occur as pregnancy progresses. [ABSTRACT FROM AUTHOR]

Published
2022
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34. Prevalence of Trichinella britovi in muscle tissue samples from foxes from Podkarpackie Voivodeship, Poland

Author

Wiśniewski, Jan, primary, Bełkot, Zbigniew, additional, Orłowska, Blanka, additional, Bogdan, Janusz, additional, Welz, Mirosław, additional, Kaczor, Stanisław, additional, Nowicki, Marek, additional, Puchalska, Martyna, additional, Tracz, Michał, additional, Różycki, Mirosław, additional, Chmurzyńska, Ewa, additional, Bilska-Zając, Ewa, additional, and Anusz, Krzysztof, additional

Published
2016
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37. Małe gryzonie jako potencjalne źródlo zakażenia dermatofitami dia człowieka i zwierząt.

Author

Orłowska, Blanka, Compa, Agnieszka, and Czujkowska, Agnieszka

Subjects
*DERMATOPHYTES, *LABORATORY rodents, *MICROBIAL contamination, *DISEASE risk factors, *MAMMAL physiology, *ANIMAL disease models, *MEDICAL mycology, *MEDICAL research, ANIMAL research
Abstract

Background: Dermatophytosis are one of the most important zoonosis. Cats are commonly believed to be the main source of those zoophilic dermatophytes. Aim: The aim of this research was to verify if small mammals, e.g. rodents or leporids can also play an important role in the spreading of dermatophytosis. Material and methods: The authors have examined 43 animals from 5 different species (guinea pig, hamster, rat, rabbit, degu). Skin and hairs specimens were collected and tested with standard mycological procedures, e.g. direct microscopic examination, fungi culture. Antimycogramms were done as well. Results: Clinical dermatophytosis and asymptomatic carriers were found. Dermatophytes were isolated from 15(34.9%) of 43 examinated animals, 4 of them were free of clinical lesions. Conclusion: Small mammals can be a source of dermathophytes and play an important role in the spreading of the disease. [ABSTRACT FROM AUTHOR]

Published
2010

38. Microbiological assessment of sheep lymph nodes with lymphadenitis found during post-mortemexamination of slaughtered sheep: implications for veterinary-sanitary meat control

Author

Didkowska, Anna, Żmuda, Piotr, Kwiecień, Ewelina, Rzewuska, Magdalena, Klich, Daniel, Krajewska-Wędzina, Monika, Witkowski, Lucjan, Żychska, Monika, Kaczmarkowska, Aleksandra, Orłowska, Blanka, and Anusz, Krzysztof

Abstract

Background: Microbiological examination of lesions found in slaughtered animals during meat inspection is an important part of public health protection as such lesions may be due to zoonotic agents that can be transmitted by meat. Examination of inflamed lymph nodes also plays a particular important role, as lymphadenitis may reflect a more widespread infection. Such lesions in sheep are mainly caused by pyogenic bacteria but also mycobacteria are occasionally found. Meat inspection data from 2017 to 2018 from southern Poland, especially from the Małopolska region, indicate that purulent or caseous lymphadenitis involving the mediastinal and tracheobronchial lymph nodes (MTLNs) is a common finding. The primary aim of the current study was to determine the aetiology of these lesions. Furthermore, it was investigated how presence of lesions was correlated with age and grazing strategy of affected sheep. Results: Post-mortemexamination revealed purulent or caseous lymphadenitis in the MTLNs of 49 out of 284 animals (17.3%). Subsequent microbiological examination revealed the presence of Corynebacterium pseudotuberculosis(34.7%), Streptococcus dysgalactiaesubsp. equisimilis(34.7%), Staphylococcus aureus(8.2%), Enterococcusspp. (2.0%), Trueperella pyogenes(2.0%), and β-haemolytic strains of Escherichia coli(2.0%). Mycobacteriumspp. and Rhodococcus equiwere not detected. In older sheep, the probability of the presence of purulent or caseous lymphadenitis was higher than in younger, and the risk was increasing by 1.5% with each month of life. Sheep grazing locally had 4.5-times greater risk of having purulent or caseous lymphadenitis than individuals summer grazing in the mountains. Conclusion: The most common aetiological agents of purulent or caseous lymphadenitis in the MTLNs of sheep in the Małopolska region were C. pseudotuberculosisand S. dysgalactiaesubsp. equisimilis. Particular attention during post-mortemexamination should be paid to the carcasses of older sheep and sheep grazing on permanent pastures, as they seem more prone to develop purulent or caseous lymphadenitis.

Published
2020
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39. Molecular characterisation of Mycobacterium capraestrains isolated in Poland

Author

Krajewska‐Wędzina, Monika, Kozińska, Monika, Orłowska, Blanka, Weiner, Marcin, Szulowski, Krzysztof, Augustynowicz‐Kopeć, Ewa, Anusz, Krzysztof, and Smith, Noel H

Abstract

Bovine tuberculosis (bovine TB, bTB) is caused by bovine bacilli: Mycobacterium bovisand M caprae. The studies conducted in Poland, in the National Bovine Tuberculosis Reference Laboratory in the Department of Microbiology of the National Veterinary Research Institute in Pulawy, show that animal tuberculosis in Poland is also caused by M caprae. We here describe the identification and genotypic assessment of 52 isolates of M capraeobtained from Polish cattle and wild animals over the last five years. We show that strains isolated from bison have significant genotypic diversity and are distinct compared with the genotypes of strains isolated from cattle. Similarly, isolates from cattle herds can be highly genotypically variable. Formal designation of the members of the Mycobacterium tuberculosiscomplex is controversial in Poland; there is a gap in veterinary legislation with regard to bTB and no explicit mention of M capraecausing tuberculosis in animal.

Published
2018
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