21 results on '"Opri R"'
Search Results
2. Surgical Antimicrobial Prophylaxis in Pediatric Patients Undergoing Plastic Surgery: A RAND/UCLA Appropriateness Method Consensus Study
- Author
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Esposito S, Sgarzani R, Bianchini S, Monaco S, Nicoletti L, Rigotti E, Di Pietro M, Opri R, Caminiti C, Ciccia M, Conti G, Donà D, Giuffré M, La Grutta S, Lancella L, Lima M, Lo Vecchio A, Pelizzo G, Piacentini G, Pietrasanta C, Puntoni M, Simonini A, Venturini E, Staiano A, Principi N, On Behalf Of The Peri-Operative Prophylaxis In Neonatal And Paediatric Age Pop-NeoPed Study Group., Esposito S., Sgarzani R., Bianchini S., Monaco S., Nicoletti L., Rigotti E., Di Pietro M., Opri R., Caminiti C., Ciccia M., Conti G., Dona D., Giuffre M., La Grutta S., Lancella L., Lima M., Vecchio A.L., Pelizzo G., Piacentini G., Pietrasanta C., Puntoni M., Simonini A., Venturini E., Staiano A., Principi N., Esposito, Susanna, Sgarzani, Rossella, Bianchini, Sonia, Monaco, Sara, Nicoletti, Laura, Rigotti, Erika, Di Pietro, Marilia, Opri, Roberta, Caminiti, Caterina, Ciccia, Matilde, Conti, Giorgio, Donà, Daniele, Giuffré, Mario, La Grutta, Stefania, Lancella, Laura, Lima, Mario, Lo Vecchio, Andrea, Pelizzo, Gloria, Piacentini, Giorgio, Pietrasanta, Carlo, Puntoni, Matteo, Simonini, Alessandro, Venturini, Elisabetta, Staiano, Annamaria, and Principi, Nicola
- Subjects
Microbiology (medical) ,Settore MED/38 - Pediatria Generale e Specialistica ,wound ,Biochemistry ,Microbiology ,antibiotics ,burn ,pediatric infectious diseases ,plastic surgery ,surgical antibiotic prophylaxis ,pediatric infectious disease ,Infectious Diseases ,antibiotic ,Pharmacology (medical) ,surgical antibiotic prophylaxi ,General Pharmacology, Toxicology and Pharmaceutics ,Keywords: antibiotic - Abstract
For many years, it was clearly shown that surgical procedures might be associated with surgical site infection (SSI). Many scientific institutions prepared guidelines to use in surgery to reduce abuse and misuse of antibiotics. However, in the general guidelines for surgical antibiotic prophylaxis, plastic surgical procedures are not addressed or are only marginally discussed, and children were almost systematically excluded. The main aim of this Consensus document is to provide clinicians with recommendations on antimicrobial prophylaxis for pediatric patients undergoing plastic surgery. The following scenarios were considered: clean plastic surgery in elective procedures with an exclusive skin and subcutis involvement; clean-contaminated/contaminated plastic surgery in elective procedures with an exclusive skin and subcutis involvement; elective plastic surgery with use of local flaps; elective plastic surgery with the use of grafts; prolonged elective plastic surgery; acute burns; clean contused lacerated wounds without bone exposure; high-risk contused lacerated wounds or with bone exposure; contused lacerated wound involving the oral mucosa; plastic surgery following human bite; plastic surgery following animal bite; plastic surgery with tissue expander insertion. Our Consensus document shows that antimicrobial perioperative prophylaxis in pediatric patients undergoing plastic surgery is recommended in selected cases. While waiting the results of further pediatric studies, the application of uniform and shared protocols in these procedures will improve surgical practice, with a reduction in SSIs and consequent rationalization of resources and costs, as well as limiting the phenomenon of antimicrobial resistance.
- Published
- 2022
3. True and false contraindications to vaccines
- Author
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Opri, R., Zanoni, G., Caffarelli, C., Bottau, P., Caimmi, S., Crisafulli, G., Franceschini, F., Liotti, L., Saretta, F., Vernich, M., and Peroni, D.G.
- Published
- 2018
- Full Text
- View/download PDF
4. A postmarket safety comparison of 2 vaccination strategies for measles, mumps, rubella and varicella in Italy
- Author
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Cocchio, Silvia, Zanoni, G, Opri, R, Russo, F, Baldo, Vincenzo, Baldovin, Tatjana, Furlan, Patrizia, Fontebasso, F, Bicciato, E, Morgillo, Gaetano, Minascurta, Veronica, Pitter, Gisella, Burmaz, Tea, Anello, Paola, Moretti, U, Gonella, L. A, Ferro, A, Ferraresso, A, Pedone, M. D, Selle, V, Destefani, E, Sforzi, M, Pupo, A, Costa, S, Vitale, V, Fassinato, V, Zivelonghi, G, and Napoletano, G.
- Subjects
Male ,Pediatrics ,medicine.medical_specialty ,Mumps measles rubella ,Drug-Related Side Effects and Adverse Reactions ,Immunology ,adverse event ,active surveillance, adverse event, febrile seizure, MMRV, MMR+V ,MMR+V ,MMRV ,active surveillance ,febrile seizure ,Rubella ,Measles ,Chickenpox Vaccine ,03 medical and health sciences ,0302 clinical medicine ,Short Reports ,Surveys and Questionnaires ,030225 pediatrics ,Product Surveillance, Postmarketing ,Humans ,Immunology and Allergy ,Medicine ,Vaccines, Combined ,030212 general & internal medicine ,Adverse effect ,Immunization Schedule ,Pharmacology ,business.industry ,Infant ,medicine.disease ,Vaccination ,Safety profile ,Systemic reaction ,Italy ,Cohort ,Female ,business ,Measles-Mumps-Rubella Vaccine - Abstract
It is strategically important to monitor the safety profile of vaccination schedules in order to achieve and maintain high levels of coverage. We analyzed the cohort of individuals actively invited for measles, mumps, rubella and varicella (MMRV) vaccination in the Veneto region (north-east Italy) from 8/1/2013 to 7/31/2014, assessing the onset of adverse events (AE) relating to 2 different vaccination strategies for MMRV (MMR+V vs MMRV). During the vaccination session at 14 months old, parents were given a form for recording local and systemic reactions to vaccinations for 4 weeks afterwards. Overall, 12,288 forms were returned, and 84.6% of them were included in this analysis (5,130 relating to MMR+V and 5,265 to MMRV); 37.3% of the sample reported no AEs, with no difference between the 2 groups. Local reactions were more common in the MMR+V group (9.6% vs 2.9%; RR 3.33; 95% CI 2.79-3.98), while there was no difference in general reactions between the 2 groups (50% MMR+V vs 52% MMRV). The events most often reported were "fever39.5°C," which was more frequently associated with the MMRV strategy (p0.001), and "skin blotches and marks," which occurred more often in the MMR+V group (p0.001). Reports of "fever ≥39.5°C" were equally distributed between the 2 groups. Sixteen cases of febrile seizures were reported (0.14% in the MMR+V group and 0.17% in the MMRV group). Similar safety profiles were identified for the 2 vaccination strategies. Although the method used to record reactions to vaccination demanded considerable resources, it enabled important information to be collected on parents' perception of the AEs occurring in response to their child's vaccination.
- Published
- 2015
5. EEG findings during “paroxysmal hemiplegia” in a patient with GLUT1-deficiency
- Author
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Pellegrin, S., primary, Cantalupo, G., additional, Opri, R., additional, Dalla Bernardina, B., additional, and Darra, F., additional
- Published
- 2017
- Full Text
- View/download PDF
6. Vaccination management in children and adults with mastocytosis
- Author
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Zanoni, G., primary, Zanotti, R., additional, Schena, D., additional, Sabbadini, C., additional, Opri, R., additional, and Bonadonna, P., additional
- Published
- 2017
- Full Text
- View/download PDF
7. Immune response to Hepatitis B vaccine in patients with celiac disease: A systematic review and meta-analysis
- Author
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Opri, R, primary, Veneri, D, additional, Mengoli, C, additional, and Zanoni, G, additional
- Published
- 2015
- Full Text
- View/download PDF
8. Adverse events following immunization,Reazioni avverse osservabili dopo vaccinazione
- Author
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Felicetti, P., Francesco Trotta, Santuccio, C., Biasiotta, A., Marchione, P., Rizzo, C., Zanoni, G., and Opri, R.
9. Antimicrobial Prophylaxis in Neonates and Children Undergoing Dental, Maxillo-Facial or Ear-Nose-Throat (ENT) Surgery: A RAND/UCLA Appropriateness Method Consensus Study
- Author
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Erika, Rigotti, Sonia, Bianchini, Laura, Nicoletti, Sara, Monaco, Elena, Carrara, Francesca, Opri, Roberta, Opri, Caterina, Caminiti, Daniele, Donà, Mario, Giuffré, Alessandro, Inserra, Laura, Lancella, Alessandro, Mugelli, Giorgio, Piacentini, Nicola, Principi, Simonetta, Tesoro, Elisabetta, Venturini, Annamaria, Staiano, Alberto, Villani, Enrico, Sesenna, Claudio, Vicini, Susanna, Esposito, On Behalf Of The Peri-Operative Prophylaxis In Neonatal And Paediatric Age Pop-NeoPed Study Group, Rigotti E., Bianchini S., Nicoletti L., Monaco S., Carrara E., Opri F., Opri R., Caminiti C., Dona D., Giuffre M., Inserra A., Lancella L., Mugelli A., Piacentini G., Principi N., Tesoro S., Venturini E., Staiano A., Villani A., Sesenna E., Vicini C., Esposito S., Rigotti, E., Bianchini, S., Nicoletti, L., Monaco, S., Carrara, E., Opri, F., Opri, R., Caminiti, C., Dona, D., Giuffre, M., Inserra, A., Lancella, L., Mugelli, A., Piacentini, G., Principi, N., Tesoro, S., Venturini, E., Staiano, A., Villani, A., Sesenna, E., Vicini, C., and Esposito, S.
- Subjects
Microbiology (medical) ,ENT surgery ,surgical antimicrobial prophylaxis ,Biochemistry ,Microbiology ,Settore MED/38 ,dental surgery ,head and neck surgery ,stomatognathic diseases ,Infectious Diseases ,maxilla-facial surgery ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics - Abstract
Surgical site infections (SSIs) represent a potential complication in surgical procedures, mainly because clean/contaminated surgery involves organs that are normally colonized by bacteria. Dental, maxillo-facial and ear-nose-throat (ENT) surgeries are among those that carry a risk of SSIs because the mouth and the first respiratory tracts are normally colonized by a bacterial flora. The aim of this consensus document was to provide clinicians with recommendations on surgical antimicrobial prophylaxis in neonates (
- Published
- 2022
10. Surgical Antimicrobial Prophylaxis in Patients of Neonatal and Pediatric Age Undergoing Orthopedic and Hand Surgery: A RAND/UCLA Appropriateness Method Consensus Study
- Author
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Francesca, Opri, Sonia, Bianchini, Laura, Nicoletti, Sara, Monaco, Roberta, Opri, Marilia, Di Pietro, Elena, Carrara, Erika, Rigotti, Cinzia, Auriti, Caterina, Caminiti, Daniele, Donà, Laura, Lancella, Andrea, Lo Vecchio, Simone, Pizzi, Nicola, Principi, Alessandro, Simonini, Simonetta, Tesoro, Eisabetta, Venturini, Alberto, Villani, Annamaria, Staiano, Leonardo, Marchesini Reggiani, Susanna, Esposito, On Behalf Of The Peri-Operative Prophylaxis In Neonatal And Paediatric Age Pop-NeoPed Study Group, Opri, F., Bianchini, S., Nicoletti, L., Monaco, S., Opri, R., Di Pietro, M., Carrara, E., Rigotti, E., Auriti, C., Caminiti, C., Dona, D., Lancella, L., Lo Vecchio, A., Pizzi, S., Principi, N., Simonini, A., Tesoro, S., Venturini, E., Villani, A., Staiano, A., Reggiani, L. M., and Esposito, S.
- Subjects
Microbiology (medical) ,Orthopedic surgery ,Antibiotic ,Hand surgery ,Biochemistry ,Microbiology ,Settore MED/38 ,antibiotics ,Infectious Diseases ,Pediatric infectious disease ,Surgical antibiotic prophylaxis ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,hand surgery ,orthopedic surgery ,pediatric infectious diseases ,surgical antibiotic prophylaxis - Abstract
Surgical site infections (SSIs) represent a potential complication in any type of surgery and can occur up to one year after the procedure in the case of implant placement. In the field of orthopedic and hand surgery, the rate of SSIs is a relevant issue, considering the need for the placement of synthesis devices and the type of some interventions (e.g., exposed fractures). This work aims to provide guidance on the management of peri-operative antibiotic prophylaxis for the pediatric and neonatal population undergoing orthopedic and hand surgery in order to standardize the management of patients and to reduce, on the one hand, the risk of SSI and, on the other, the development of antimicrobial resistance. The following scenarios were considered: (1) bloodless fracture reduction; (2) reduction of unexposed fracture and grade I and II exposed fracture; (3) reduction of grade III exposed fracture or traumatic amputation; (4) cruel fracture reduction with percutaneous synthesis; (5) non-traumatic amputation; (6) emergency intact skin trauma surgery and elective surgery without synthetic media placement; (7) elective orthopedic surgery with prosthetic and/or synthetic media placement and spinal surgery; (8) clean elective hand surgery with and without bone involvement, without use of synthetic means; (9) surgery of the hand on an elective basis with bone involvement and/or with use of synthetic means. This manuscript has been made possible by the multidisciplinary contribution of experts belonging to the most important Italian scientific societies and represents, in our opinion, the most complete and up-to-date collection of recommendations regarding the behavior to be adopted in the peri-operative setting in neonatal and pediatric orthopedic and hand surgery. The specific scenarios developed are aimed at guiding the healthcare professional in practice to ensure the better and standardized management of neonatal and pediatric patients, together with an easy consultation.
- Published
- 2022
11. The presence of glutamate residues on the PAS sequence of the stimuli-sensitive nano-ferritin improves in vivo biodistribution and mitoxantrone encapsulation homogeneity
- Author
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Roberta Opri, Francesco Fazi, Gianni Colotti, Elisa Tremante, Aldo Braca, Francesca Malagrinò, Elisabetta Falvo, Patrizio Di Micco, Giulio Fracasso, Alessandro Arcovito, Pierpaolo Ceci, Alessandro Giuffrè, Falvo, E., Malagrino, Francesca., Arcovito, A., Fazi, F., Colotti, G., Tremante, E., Di Micco, P., Braca, A., Opri, R., Giuffre, A., Fracasso, G., and Ceci, P.
- Subjects
0301 basic medicine ,Biodistribution ,Proteases ,Glutamic Acid ,Pharmaceutical Science ,Antineoplastic Agents ,02 engineering and technology ,Serine ,03 medical and health sciences ,Doxorubicin (PubChem CID:31703) ,In vivo ,Cell Line, Tumor ,Protein-cage nanocarrier ,Pasylated ferritin ,Humans ,Tissue Distribution ,cancer ,drug-delivery ,drug-encapsulation ,mitoxantrone ,pasylated ferritin ,protein-cage nanocarrier ,3003 ,Settore BIO/10 - BIOCHIMICA ,Peptide sequence ,Cancer ,Drug Carriers ,Chemistry ,Mitoxantrone (PubChem CID:4212) ,Drug-delivery ,Drug-encapsulation ,Mitoxantrone ,021001 nanoscience & nanotechnology ,In vitro ,030104 developmental biology ,Biochemistry ,Doxorubicin ,Apoferritins ,Cancer cell ,Drug delivery ,Nanoparticles ,0210 nano-technology - Abstract
A genetically engineered human ferritin heavy chain (HFt)-based construct has been recently shown by our group to efficiently entrap and deliver doxorubicin to cancer cells. This construct, named HFt-MP-PAS, contained a tumor-selective sequence (MP) responsive to proteolytic cleavage by tumor proteases (MMPs), located between each HFt subunit and an outer shielding polypeptide sequence rich in proline (P), serine (S) and alanine (A) residues (PAS). HFt-MP-PAS displayed excellent therapeutic efficacy in xenogenic pancreatic and head and neck cancer models in vivo, leading to a significant increase in overall animal survivals. Here we report a new construct obtained by the genetic insertion of two glutamate residues in the PAS sequence of HFt-MP-PAS. Such new construct, named HFt-MP-PASE, is characterized by improved performances as drug biodistribution in a xenogenic pancreatic cancer model in vivo. Moreover, HFt-MP-PASE efficiently encapsulates the anti-cancer drug mitoxantrone (MIT), and the resulting MIT-loaded nanoparticles proved to be more soluble and monodispersed than the HFt-MP-PAS counterparts. Importantly, in vitro MIT-loaded HFt-MP-PASE kills several cancer cell lines of different origin (colon, breast, sarcoma and pancreas) at least as efficiently as the free drug. Finally, our MIT loaded protein nanocages allowed in vivo an impressive incrementing of the drug accumulation in the tumor with respect to the free drug.
- Published
- 2018
- Full Text
- View/download PDF
12. Antimicrobial Prophylaxis in Neonates and Children Undergoing Dental, Maxillo-Facial or Ear-Nose-Throat (ENT) Surgery: A RAND/UCLA Appropriateness Method Consensus Study.
- Author
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Rigotti E, Bianchini S, Nicoletti L, Monaco S, Carrara E, Opri F, Opri R, Caminiti C, Donà D, Giuffré M, Inserra A, Lancella L, Mugelli A, Piacentini G, Principi N, Tesoro S, Venturini E, Staiano A, Villani A, Sesenna E, Vicini C, Esposito S, and On Behalf Of The Peri-Operative Prophylaxis In Neonatal And Paediatric Age Pop-NeoPed Study Group
- Abstract
Surgical site infections (SSIs) represent a potential complication in surgical procedures, mainly because clean/contaminated surgery involves organs that are normally colonized by bacteria. Dental, maxillo-facial and ear-nose-throat (ENT) surgeries are among those that carry a risk of SSIs because the mouth and the first respiratory tracts are normally colonized by a bacterial flora. The aim of this consensus document was to provide clinicians with recommendations on surgical antimicrobial prophylaxis in neonates (<28 days of chronological age) and pediatric patients (within the age range of 29 days−18 years) undergoing dental, maxillo-facial or ENT surgical procedures. These included: (1) dental surgery; (2) maxilla-facial surgery following trauma with fracture; (3) temporo-mandibular surgery; (4) cleft palate and cleft lip repair; (5) ear surgery; (6) endoscopic paranasal cavity surgery and septoplasty; (7) clean head and neck surgery; (8) clean/contaminated head and neck surgery and (9) tonsillectomy and adenoidectomy. Due to the lack of pediatric data for the majority of dental, maxillo-facial and ENT surgeries and the fact that the recommendations for adults are currently used, there is a need for ad hoc studies to be rapidly planned for the most deficient areas. This seems even more urgent for interventions such as those involving the first airways since the different composition of the respiratory microbiota in children compared to adults implies the possibility that surgical antibiotic prophylaxis schemes that are ideal for adults may not be equally effective in children.
- Published
- 2022
- Full Text
- View/download PDF
13. Surgical Antimicrobial Prophylaxis in Patients of Neonatal and Pediatric Age Undergoing Orthopedic and Hand Surgery: A RAND/UCLA Appropriateness Method Consensus Study.
- Author
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Opri F, Bianchini S, Nicoletti L, Monaco S, Opri R, Di Pietro M, Carrara E, Rigotti E, Auriti C, Caminiti C, Donà D, Lancella L, Lo Vecchio A, Pizzi S, Principi N, Simonini A, Tesoro S, Venturini E, Villani A, Staiano A, Marchesini Reggiani L, Esposito S, and On Behalf Of The Peri-Operative Prophylaxis In Neonatal And Paediatric Age Pop-NeoPed Study Group
- Abstract
Surgical site infections (SSIs) represent a potential complication in any type of surgery and can occur up to one year after the procedure in the case of implant placement. In the field of orthopedic and hand surgery, the rate of SSIs is a relevant issue, considering the need for the placement of synthesis devices and the type of some interventions (e.g., exposed fractures). This work aims to provide guidance on the management of peri-operative antibiotic prophylaxis for the pediatric and neonatal population undergoing orthopedic and hand surgery in order to standardize the management of patients and to reduce, on the one hand, the risk of SSI and, on the other, the development of antimicrobial resistance. The following scenarios were considered: (1) bloodless fracture reduction; (2) reduction of unexposed fracture and grade I and II exposed fracture; (3) reduction of grade III exposed fracture or traumatic amputation; (4) cruel fracture reduction with percutaneous synthesis; (5) non-traumatic amputation; (6) emergency intact skin trauma surgery and elective surgery without synthetic media placement; (7) elective orthopedic surgery with prosthetic and/or synthetic media placement and spinal surgery; (8) clean elective hand surgery with and without bone involvement, without use of synthetic means; (9) surgery of the hand on an elective basis with bone involvement and/or with use of synthetic means. This manuscript has been made possible by the multidisciplinary contribution of experts belonging to the most important Italian scientific societies and represents, in our opinion, the most complete and up-to-date collection of recommendations regarding the behavior to be adopted in the peri-operative setting in neonatal and pediatric orthopedic and hand surgery. The specific scenarios developed are aimed at guiding the healthcare professional in practice to ensure the better and standardized management of neonatal and pediatric patients, together with an easy consultation.
- Published
- 2022
- Full Text
- View/download PDF
14. Mitoxantrone-Loaded Nanoferritin Slows Tumor Growth and Improves the Overall Survival Rate in a Subcutaneous Pancreatic Cancer Mouse Model.
- Author
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Conti G, Pitea M, Ossanna R, Opri R, Tisci G, Falvo E, Innamorati G, Ghanem E, Sbarbati A, Ceci P, and Fracasso G
- Abstract
Pancreatic cancer (PC) represents an intriguing topic for researchers. To date, the prognosis of metastasized PC is poor with just 7% of patients exceeding a five-year survival period. Thus, molecular modifications of existing drugs should be developed to change the course of the disease. Our previously generated nanocages of Mitoxantrone (MIT) encapsulated in human H-chain Ferritin (HFt), designated as HFt-MP-PASE-MIT, has shown excellent tumor distribution and extended serum half-life meriting further investigation for PC treatment. Thus, in this study, we used the same nano-formulation to test its cytotoxicity using both in vitro and in vivo assays. Interestingly, both encapsulated and free-MIT drugs demonstrated similar killing capabilities on PaCa44 cell line. Conversely, in vivo assessment in a subcutaneous PaCa44 tumor model of PC demonstrated a remarkable capability for encapsulated MIT to control tumor growth and improve mouse survival with a median survival rate of 65 vs. 33 days for loaded and free-MIT, respectively. Interestingly, throughout the course of mice treatment, MIT encapsulation did not present any adverse side effects as confirmed by histological analysis of various murine tissue organs and body mass weights. Our results are promising and pave the way to effective PC targeted chemotherapy using our HFt nanodelivery platforms.
- Published
- 2021
- Full Text
- View/download PDF
15. Migrating Focal Seizures and Myoclonic Status in ARV1- Related Encephalopathy.
- Author
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Darra F, Lo Barco T, Opri R, Parrini E, Bianchini C, Fiorini E, Simonati A, Dalla Bernardina B, Cantalupo G, and Guerrini R
- Abstract
Objective: To report longitudinal clinical, EEG, and MRI findings in 2 sisters carrying compound heterozygous ARV1 mutations and exhibiting a peculiar form of developmental and epileptic encephalopathy (DEE). Neuropathologic features are also described in one of the sisters., Methods: Clinical course description, video-EEG polygraphic recordings, brain MRI, skin and muscle biopsies, whole-exome sequencing (WES), and brain neuropathology., Results: Since their first months of life, both girls exhibited severe axial hypotonia, visual inattention, dyskinetic movements, severe developmental delay, and slow background EEG activity. Intractable nonmotor seizures started in both at the eighth month of life, exhibiting the electroclinical characteristics of epilepsy of infancy with migrating focal seizures (EIMFS). In the second year of life, continuous epileptiform EEG activity of extremely high amplitude appeared in association with myoclonic status, leading to severely impaired alertness and responsiveness. Repeated brain MRI revealed progressive atrophic changes and severe hypomyelination. WES identified a compound heterozygous in the ARV1 gene [(p.Ser122Glnfs*7) and (p.Trp163*)] in one patient and was subsequently confirmed in the other. Both sisters died prematurely during respiratory infections. Postmortem neuropathologic examination of the brain, performed in one, revealed atrophic brain changes, mainly involving the cerebellum., Conclusions: This report confirms that biallelic ARV1 mutations cause a severe form of DEE and adds epilepsy with migrating focal seizures and myoclonic status to the spectrum of epilepsy phenotypes. Considering the potential role of human ARV1 in glycosylphosphatidylinositol (GPI) anchor biosynthesis, this severe syndrome can be assigned to the group of inherited GPI deficiency disorders, with which it shares remarkably similar clinical and neuroimaging features. ARV1 should be considered in the genetic screening of individuals with EIMFS., (Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.)
- Published
- 2021
- Full Text
- View/download PDF
16. The presence of glutamate residues on the PAS sequence of the stimuli-sensitive nano-ferritin improves in vivo biodistribution and mitoxantrone encapsulation homogeneity.
- Author
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Falvo E, Malagrinò F, Arcovito A, Fazi F, Colotti G, Tremante E, Di Micco P, Braca A, Opri R, Giuffrè A, Fracasso G, and Ceci P
- Subjects
- Cell Line, Tumor, Humans, Tissue Distribution, Antineoplastic Agents administration & dosage, Apoferritins administration & dosage, Doxorubicin administration & dosage, Drug Carriers administration & dosage, Glutamic Acid administration & dosage, Mitoxantrone administration & dosage, Nanoparticles administration & dosage
- Abstract
A genetically engineered human ferritin heavy chain (HFt)-based construct has been recently shown by our group to efficiently entrap and deliver doxorubicin to cancer cells. This construct, named HFt-MP-PAS, contained a tumor-selective sequence (MP) responsive to proteolytic cleavage by tumor proteases (MMPs), located between each HFt subunit and an outer shielding polypeptide sequence rich in proline (P), serine (S) and alanine (A) residues (PAS). HFt-MP-PAS displayed excellent therapeutic efficacy in xenogenic pancreatic and head and neck cancer models in vivo, leading to a significant increase in overall animal survivals. Here we report a new construct obtained by the genetic insertion of two glutamate residues in the PAS sequence of HFt-MP-PAS. Such new construct, named HFt-MP-PASE, is characterized by improved performances as drug biodistribution in a xenogenic pancreatic cancer model in vivo. Moreover, HFt-MP-PASE efficiently encapsulates the anti-cancer drug mitoxantrone (MIT), and the resulting MIT-loaded nanoparticles proved to be more soluble and monodispersed than the HFt-MP-PAS counterparts. Importantly, in vitro MIT-loaded HFt-MP-PASE kills several cancer cell lines of different origin (colon, breast, sarcoma and pancreas) at least as efficiently as the free drug. Finally, our MIT loaded protein nanocages allowed in vivo an impressive incrementing of the drug accumulation in the tumor with respect to the free drug., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
17. Immune Response to Rotavirus and Gluten Sensitivity.
- Author
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Puccetti A, Saverino D, Opri R, Gabrielli O, Zanoni G, Pelosi A, Fiore PF, Moretta F, Lunardi C, and Dolcino M
- Subjects
- Adult, Autoantibodies blood, Autoimmunity, Child, Preschool, Diarrhea, Female, Gene Expression Profiling, Humans, Male, Autoimmune Diseases immunology, Celiac Disease immunology, Glutens immunology, Rotavirus immunology, Rotavirus Infections immunology, Th17 Cells immunology
- Abstract
Rotavirus is a double-stranded RNA virus belonging to the family of Reoviridae . The virus is transmitted by the faecal-oral route and infects intestinal cells causing gastroenteritis. Rotaviruses are the main cause of severe acute diarrhoea in children less than 5 years of age worldwide. In our previous work we have shown a link between rotavirus infection and celiac disease. Nonceliac gluten sensitivity (NCGS) is emerging as new clinical entity lacking specific diagnostic biomarkers which has been reported to occur in 6-10% of the population. Clinical manifestations include gastrointestinal and/or extraintestinal symptoms which recede with gluten withdrawal. The pathogenesis of the disease is still unknown. Aim of this work is to clarify some aspects of its pathogenesis using a gene array approach. Our results suggest that NCGS may have an autoimmune origin. This is based both on gene expression data (i.e., TH17-interferon signatures) and on the presence of TH17 cells and of serological markers of autoimmunity in NCGS. Our results also indicate a possible involvement of rotavirus infection in the pathogenesis of nonceliac gluten sensitivity similarly to what we have previously shown in celiac disease.
- Published
- 2018
- Full Text
- View/download PDF
18. Targeted killing of prostate cancer cells using antibody-drug conjugated carbon nanohorns.
- Author
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Lucío MI, Opri R, Pinto M, Scarsi A, Fierro JLG, Meneghetti M, Fracasso G, Prato M, Vázquez E, and Herrero MA
- Abstract
The ability of carbon nanohorns (CNHs) to cross biological barriers makes them potential carriers for delivery purposes. In this work, we report the design of a new selective antibody-drug nanosystem based on CNHs for the treatment of prostate cancer (PCa). In particular, cisplatin in a prodrug form and the monoclonal antibody (Ab) D2B, selective for PSMA
+ cancer cells, have been attached to CNHs due to the current application of this antigen in PCa therapy. The hybrids Ab-CNHs, cisplatin-CNHs and functionalised-CNHs have also been synthesized to be used as control systems. The efficacy and specificity of the D2B-cisplatin-CNH conjugate to selectively target and kill PSMA+ prostate cancer cells have been demonstrated in comparison with other derivatives. The developed strategy to functionalise CNHs is fascinating because it can allow the fine tuning of both drug and Ab molecules attached to the nanostructure in order to modulate the activity of the nanosystem. Finally, the herein described methodology can be used for the incorporation of almost any drugs or Abs in the platforms in order to create new targeted drugs for the treatment of different diseases.- Published
- 2017
- Full Text
- View/download PDF
19. Single organ cutaneous vasculitis: Case definition & guidelines for data collection, analysis, and presentation of immunization safety data.
- Author
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Zanoni G, Girolomoni G, Bonetto C, Trotta F, Häusermann P, Opri R, and Bonhoeffer J
- Subjects
- Data Collection, Guidelines as Topic, Humans, Skin Diseases diagnosis, Skin Diseases therapy, Terminology as Topic, Vasculitis diagnosis, Vasculitis therapy, Immunization adverse effects, Skin Diseases epidemiology, Skin Diseases pathology, Vasculitis epidemiology, Vasculitis pathology
- Published
- 2016
- Full Text
- View/download PDF
20. Progressive Myoclonus Epilepsy in Congenital Generalized Lipodystrophy type 2: Report of 3 cases and literature review.
- Author
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Opri R, Fabrizi GM, Cantalupo G, Ferrarini M, Simonati A, Dalla Bernardina B, and Darra F
- Subjects
- Brain diagnostic imaging, Carrier Proteins genetics, Child, Diagnosis, Differential, Fatal Outcome, Female, Humans, Lipodystrophy, Congenital Generalized diagnosis, Lipodystrophy, Congenital Generalized pathology, Male, Myoclonic Epilepsies, Progressive diagnosis, Myoclonic Epilepsies, Progressive pathology, Protein Tyrosine Phosphatases, Non-Receptor genetics, Skin metabolism, Skin pathology, Ubiquitin-Protein Ligases, Brain physiopathology, GTP-Binding Protein gamma Subunits genetics, Lipodystrophy, Congenital Generalized genetics, Lipodystrophy, Congenital Generalized physiopathology, Myoclonic Epilepsies, Progressive genetics, Myoclonic Epilepsies, Progressive physiopathology
- Abstract
Purpose: A small case series with a neurodegenerative disorder involving central nervous system and related to Seipin mutations was recently reported. Herein we describe clinical and EEG features of three patients presenting with Progressive Myoclonus Epilepsy (PME) and Congenital Generalized Lipodystrophy type 2 (CGL2) related to novel Seipin mutations., Methods: The EEG-clinical picture was evaluated at epilepsy onset and in the follow-up period. The molecular analysis of BSCL2, Laforin and Malin genes was performed to patients and/or their parents by Denaturing High Performance Liquid Chromatography and automated nucleotide sequencing. Skin specimens collected from a patient were processed for histochemical and ultrastructural analysis., Results: The CGL2-PME syndrome co-segregated with two different BSCL2 genotypes: the homozygosity for c.782_783dupG involving exon 8 (two cases), or the compound heterozygosity for c.782_783dupG/c.828_829delAA (one case). Periodic-Acid Schiff positive osmiophilic material in the cytoplasm of fibrocytes and eccrine-gland cells were found in skin specimens. The lack of Lafora's bodies in skin specimens and the molecular analysis excluding mutations in Laforin and Malin genes ruled out Lafora disease., Conclusion: The spectrum of CGL2 associated to BSCL2 gene mutations may include PMEs. Selected mutations in BSCL2 gene seem to be related to PMEs in patients with CGL2 phenotype., (Copyright © 2016 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
21. A postmarket safety comparison of 2 vaccination strategies for measles, mumps, rubella and varicella in Italy.
- Author
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Cocchio S, Zanoni G, Opri R, Russo F, and Baldo V
- Subjects
- Female, Humans, Infant, Italy, Male, Surveys and Questionnaires, Vaccines, Combined administration & dosage, Vaccines, Combined adverse effects, Chickenpox Vaccine administration & dosage, Chickenpox Vaccine adverse effects, Drug-Related Side Effects and Adverse Reactions epidemiology, Drug-Related Side Effects and Adverse Reactions pathology, Immunization Schedule, Measles-Mumps-Rubella Vaccine administration & dosage, Measles-Mumps-Rubella Vaccine adverse effects, Product Surveillance, Postmarketing
- Abstract
It is strategically important to monitor the safety profile of vaccination schedules in order to achieve and maintain high levels of coverage. We analyzed the cohort of individuals actively invited for measles, mumps, rubella and varicella (MMRV) vaccination in the Veneto region (north-east Italy) from 8/1/2013 to 7/31/2014, assessing the onset of adverse events (AE) relating to 2 different vaccination strategies for MMRV (MMR+V vs MMRV). During the vaccination session at 14 months old, parents were given a form for recording local and systemic reactions to vaccinations for 4 weeks afterwards. Overall, 12,288 forms were returned, and 84.6% of them were included in this analysis (5,130 relating to MMR+V and 5,265 to MMRV); 37.3% of the sample reported no AEs, with no difference between the 2 groups. Local reactions were more common in the MMR+V group (9.6% vs 2.9%; RR 3.33; 95% CI 2.79-3.98), while there was no difference in general reactions between the 2 groups (50% MMR+V vs 52% MMRV). The events most often reported were "fever <39.5°C," which was more frequently associated with the MMRV strategy (p<0.001), and "skin blotches and marks," which occurred more often in the MMR+V group (p<0.001). Reports of "fever ≥39.5°C" were equally distributed between the 2 groups. Sixteen cases of febrile seizures were reported (0.14% in the MMR+V group and 0.17% in the MMRV group). Similar safety profiles were identified for the 2 vaccination strategies. Although the method used to record reactions to vaccination demanded considerable resources, it enabled important information to be collected on parents' perception of the AEs occurring in response to their child's vaccination.
- Published
- 2016
- Full Text
- View/download PDF
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