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4 results on '"Ono, Fuka"'

2. Production of recombinant His-tagged triple-FLAG peptide in Brevibacillus choshinensisand its utilization as an easy-to-remove affinity peptide

Author

Sugihara, Daiki, Ono, Fuka, Sugino, Motoki, Suzuki, Hiromi, Endo, Noriko, Shimada, Atsuhiro, and Ebihara, Akio

Abstract

Triple-FLAG (3 × FLAG)-tagged proteins can be affinity purified through binding to an anti-FLAG antibody and competitive elution with excess free 3 × FLAG peptide. To expand the availability of the 3 × FLAG purification system, we produced a recombinant His-tagged 3 × FLAG peptide in Brevibacillus choshinensis. The screening of connecting linkers between His-tag and the 3 × FLAG peptide, culture containers, and culture media showed that the His-tagged 3 × FLAG peptide with an LA linker was most expressed in 2SY medium using a baffled shake flask. The peptide was affinity-purified to give a yield of about 25 mg/L of culture. The peptide was effective for eluting 3 × FLAG-tagged α-amylase from anti-FLAG magnetic beads. Finally, the peptide remaining in the amylase fraction was removed by His-tag affinity purification. These results show that the recombinant His-tagged 3 × FLAG peptide can function as an easy-to-remove affinity peptide in the 3 × FLAG purification system.Graphical AbstractThe recombinant His-tagged 3 × FLAG peptide produced using the Brevibacillusexpression system can expand the utility of the 3 × FLAG purification system.

Published
2023
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4. A Study on Characteristics of Cooling Heat Transfer of Supercritical Pressure Fluids in a Plate Heat Exchanger.

Author

Mori, Hideo, Nakamura, Shin, Ono, Fuka, Kariya, Keishi, and Umezawa, Shuichi

Subjects
ADSORPTION kinetics, THERMODYNAMICS of heat exchangers, THERMAL conductivity, HEAT transfer, THERMAL properties, COOLING towers
Abstract

For the development of industrial heat pump systems supplying a high-temperature heat source over 130°C, experiments were carried out on cooling heat transfer of supercritical pressure fluids flowing in a plate heat exchanger (PHE). Using two refrigerants of HFC134a and HCFC22 as the test fluids, heat transfer coefficient data were obtained at different pressure, flow rate, and heat load conditions. The heat transfer coefficient generally had a maximum in the vicinity of the pseudocritical point and showed seven- to ninefold values compared with tube flow. Based on the measurements, characteristics of cooling heat transfer of supercritical pressure fluids in the PHE were clarified and a correlation of heat transfer coefficient was developed. [ABSTRACT FROM AUTHOR]

Published
2016
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