1. Onium- and Alkyl Amine-Decorated Protein Nanoparticles as Antimicrobial Agents and Carriers of Antibiotics to Promote Synergistic Antibacterial and Antibiofilm Activities.
- Author
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Patel, Anjali, Paul, Suman, Akhtar, Nasim, Das, Sribash, Kar, Sajal, Bhattacharjee, Surajit, and Manna, Debasis
- Abstract
The unprecedented cases of antimicrobial resistance and scarcity of effective antibiotics against resistant strains demand the development of proficient antibiotics and their stewardship. The antibiotic carriers and the adjuvants that can counteract the resistance mechanism and revive the activity of existing antibiotics are considered as one of the promising tools to fight against antimicrobial resistance and its consequences. Herein, we demonstrated the development of sulfonium- and lauryl amine-conjugated BSA protein nanoparticles (PNPs) with inherent antimicrobial activities that embraced the benefits of biocompatibility and antibiotic-carrying capability. The PNPs showed encapsulation and controlled release profiles of clinically approved antibiotics. The antibiotic-encapsulated PNPs exhibited synergistic antimicrobial activity against Gram-negative, Gram-positive, and drug-resistant bacterial strains, which could reduce the effective doses of antibiotics and exposure to other microbial strains. Subsequent studies showed that the PNPs were capable enough to breach the sturdy biofilm barriers of the bacterial strains, and at a minimum inhibitory concentration, the biofilm lost its viability. The antibiotic-encapsulated PNPs also lower the drug resistance ability of commercial antibiotics. The mechanistic studies revealed that the antibacterial activity of the PNPs follows a membrane-directed pathway. The PNPs showed negligible toxicity against erythrocytes. Interestingly, lauryl amine and sulfonium-conjugated albumin protein (R-BSA-S) gives protection against Staphylococcus aureus biofilm-associated infection in albino laboratory-bred (BALB/c) mice, as appeared from the decrease in the colony forming unit (CFU) count and histological changes in the liver and spleen. Thus, the synthesized antimicrobial carrier molecule revitalizes the activity of the antibiotics and is a cost-effective strategy. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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