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233 results on '"Omoto Y"'

1. Panel Discussion

Author

Omoto, Y., Fukuoka, Y., Schelling, T., Tsunogai, S., van der Staal, P., Lincoln, C., Krupp, H., Takeuchi, Kei, editor, and Yoshino, Masatoshi, editor

Published
1991
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38. Generation of ERα-floxed and knockout mice using the Cre/LoxP system

Author

Antonson, P., Omoto, Y., Humire, P., and Gustafsson, J.-Å.

Subjects
*ESTROGEN receptors, *INFERTILITY, *NUCLEAR receptors (Biochemistry), *LABORATORY mice, *GENETIC regulation, *CYSTS (Pathology), *BODY weight
Abstract

Abstract: Estrogen receptor alpha (ERα) is a nuclear receptor that regulates a range of physiological processes in response to estrogens. In order to study its biological role, we generated a floxed ERα mouse line that can be used to knock out ERα in selected tissues by using the Cre/LoxP system. In this study, we established a new ERα knockout mouse line by crossing the floxed ERα mice with Cre deleter mice. Here we show that genetic disruption of the ERα gene in all tissues results in sterility in both male and female mice. Histological examination of uterus and ovaries revealed a dramatically atrophic uterus and hemorrhagic cysts in the ovary. These results suggest that infertility in female mice is the result of functional defects of the reproductive tract. Moreover, female knockout mice are hyperglycemic, develop obesity and at the age of 4months the body weight of these mice was more than 20% higher compared to wild type littermates and this difference increased over time. Our results demonstrate that ERα is necessary for reproductive tract development and has important functions as a regulator of metabolism in females. [Copyright &y& Elsevier]

Published
2012
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39. International Congress on Hormonal Steroids and Hormones and Cancer: The expression and function of estrogen receptor α and β in human breast cancer and its clinical application

Author

Hayashi, S-I, Eguchi, H, Tanimoto, K, Yoshida, T, Omoto, Y, Inoue, A, Yoshida, N, and Yamaguchi, Y

Abstract

The overexpression of estrogen receptor α (ERα) is frequently observed in the early stage of breast cancer. We previously reported that the specific promoter of the ERα gene is responsible for this enhanced transcription of the gene, and identified the cis-acting elements which play an important role in its transcription. Furthermore, methylation of the ERα gene promoters also contribute to the regulation of gene transcription. Elucidation of these mechanisms of ERα gene expression may provide useful information for the early detection and chemoprevention of breast cancer. On the other hand, the expression of ERβ has been reported in breast cancer. We have also assessed the significance and function of ERβ and its variant types in breast cancer, and suggest that ERβ and ERβcx specifically suppress the function of ERα through different mechanisms. ERβ isoforms may be important functional modulators of the estrogen-signaling pathway in breast cancer cells, and might affect the clinical outcome of patients. Moreover, to address the role of these ERs on the estrogen-dependent growth of breast cancer cells and to develop a diagnostic tool, we have analyzed the gene expression profiles of estrogen-responsive genes using cDNA microarray. Based on these results, the expression of several candidate genes in breast cancer tissues were analyzed by real-time RT-PCR and by immunohistochemical techniques, in order to discover new predictive factors for the endocrine therapy of patients with breast cancer. These studies could provide new clues for the elucidation of the estrogen-dependent mechanisms of cancer and the clinical benefits for patients.

Published
2003

41. CD4 T-lymphocyte activation is associated with peak expiratory flow variability in childhood asthma

Author

Doi, S., Murayama, N., Inoue, T., Takamatsu, I., Kameda, M., Omoto, Y., and Toyoshima, K.

Abstract

Background: Asthma has been recognized as a chronic inflammatory disorder of the airway. We have investigated the relationships among the activation markers on lymphocytes, eosinophils, their serum products in the peripheral blood, and the variability of airway obstruction in childhood asthma. Methods: Twenty-two patients with atopic asthma (mean age, 12 years) were treated regularly and asked to measure their peak expiratory flow (PEF) twice daily for 7 days. Peripheral venous blood was obtained on day 8. Results: The absolute counts of CD4 T lymphocytes expressing the activation marker CD25 in the peripheral blood on day 8 correlated significantly with the values of the coefficient of variation (CV) of both morning PEF (p<0.01) and night PEF (p<0.05) obtained over 7 days, but those of CD8+/CD25+ T lymphocytes, those of CD23+ B lymphocytes, and the serum concentrations of soluble CD25 did not. The absolute counts of peripheral blood eosinophils also demonstrated a significant correlation with the CV values of both morning PEF (p<0.01) and night PEF (p<0.05). Conclusion: CD4 T-lymphocyte activation and increased counts of eosinophils in peripheral blood correlate with CV of PEF in patients with asthma, suggesting that CV of PEF is a good marker for assessing not only the variability of airway obstruction but also the degree of airway inflammation

Published
1996
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