Search

Your search keyword '"Omotehara Y"' showing total 3 results
Start Over Author "Omotehara Y"
3 results on '"Omotehara Y"'

3. Glutamate agonist causes irreversible degeneration of inner hair cells.

Author

Hyodo J, Hakuba N, Hato N, Takeda S, Okada M, Omotehara Y, and Gyo K

Subjects
Animals, Cell Count, Cell Death, Dendrites drug effects, Dendrites physiology, Dose-Response Relationship, Drug, Evoked Potentials, Auditory, Brain Stem drug effects, Evoked Potentials, Auditory, Brain Stem physiology, Female, Gerbillinae, Hair Cells, Auditory, Inner cytology, Scala Tympani drug effects, Time Factors, Vacuoles drug effects, Excitatory Amino Acid Agonists toxicity, Hair Cells, Auditory, Inner drug effects, Hair Cells, Auditory, Inner physiology, Neurodegenerative Diseases chemically induced, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid toxicity
Abstract

Glutamate neurotoxicity in cochlear hair cells was investigated by administering the glutamate agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) into the scala tympani of Mongolian gerbils. AMPA administration caused the formation of large number of vacuoles in the inner hair cells (IHCs) and dendritic terminals. The number of degenerated hair cells was counted using rhodamine-phalloidin and Hoechst 33342 staining. The administration of 50 microM AMPA caused reversible elevation of the auditory brainstem response threshold without loss of IHCs. In contrast, 200 microM AMPA induced a substantial elevation of the auditory brainstem response threshold with the characteristic disappearance of IHCs. As cochlear ischemia involves excessive glutamate release, these results suggest that an elevated glutamate level in the cochlea is responsible for the progressive IHC death related to ischemic injury.

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results