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4 results on '"Om, Zemlianko"'

1. [Characterization of missense mutations in the SUP45 gene of Saccharomyces cerevisiae encoding translation termination factor eRF1]

Author

Se, Moskalenko, Ga, Zhuravleva, Soom MIa, Sv, Shabel Skaia, Kv, Volkov, Om, Zemlianko, Philippe M, ludmila mironova, and Sg, Inge-Vechtomov

Subjects
Saccharomyces cerevisiae Proteins, Amino Acid Substitution, Two-Hybrid System Techniques, Molecular Sequence Data, Codon, Terminator, Mutation, Missense, Amino Acid Sequence, Saccharomyces cerevisiae, Peptide Chain Termination, Translational, Peptide Termination Factors
Abstract

Collection of missense mutations in the SUP45 gene of Saccharomyces cerevisiae encoding translation termination factor eRF1 has been obtained by different approaches. It has been shown that most of isolated mutations cause amino acid substitutions in the N-terminal part of eRF1 and do not decrease the eRF1 amount. Most of mutations studied do not abolish eRF1-eRF3 interaction. The role of the N-terminal part of eRF1 in stop codon recognition is discussed.

Published
2004

2. [The identification of Saccharomyces cerevisiae genes leading to synthetic lethality of prion [PSI+] with Sup45 mutations].

Author

Matveenko AG, Zemlianko OM, and Zhuravleva GA

Subjects
Diploidy, Gene Expression Regulation, Fungal, Gene Library, Genes, Lethal, HSP40 Heat-Shock Proteins genetics, Minichromosome Maintenance 1 Protein genetics, Peptide Elongation Factor 1, Peptide Elongation Factors genetics, Prions genetics, Protein Biosynthesis, Mutation, Peptide Termination Factors genetics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics
Abstract

Previously, we proposed a test system allowing to perform search for genes that influence the properties of the Sup35 and Sup45 protein. This test is based on the phenomenon of lethality of diploids that combine mutations in SUP45 gene with [PSI+] prion. Lethality of this combination depends on the type of sup45 mutation, and the properties of the prion. [PSI+] variant, which is a strong suppressor ([PSI+]s), showing synthetic lethality with all the nonsense mutations and some missense sup45 mutations in the heterozygote state. The presence of extra copies of a gene under test that affects the phenotypic manifestation of prion [PSI+] or translation termination factors properties, leads to the increase or decrease in diploid lethality. Gene library screening using this test system allowed us to establish the effect of ten fragments of genomic DNA of yeast on synthetic lethality. Deletion analysis of these regions has led to the identification of the HLJ1 and TEF2 genes, as affecting Sup35 protein prionization and/or the efficiency of translation termination.

Published
2013
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3. [Conservation of the MC domains in eukaryotic termination factor eRF3].

Author

Zhuravleva GA, Zemlianko OM, Le Goff C, Petrova AV, Philippe M, and Inge-Vechtomov SG

Subjects
Animals, Codon, Terminator, Humans, Mice, Mutation, Peptide Termination Factors metabolism, Prions genetics, Prions metabolism, Protein Structure, Tertiary, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Xenopus Proteins genetics, Xenopus Proteins metabolism, Xenopus laevis, Peptide Termination Factors genetics
Abstract

Eukaryotic translation termination employs two protein factors, eRF1 and eRF3. Proteins of the eRF3 family each consist of three domains. The N and M domains vary in different species, while the C domains are highly homologous. The MC domains of Homo sapiens eRF3a (hGSPT I), Xenopus laevis eRF3 (XSup35), and Mus musculus eRF3a (mGSPTI) and eRF3b (mGSPT2) were found to compensate for the sup35-21(ts) temperature-sensitive mutation and lethal disruption of the SUP35 gene in yeast Saccharomyces cerevisiae. At the same time, strains containing the MC domains of the eRF3 proteins from different species differed in growth rate and the efficiency of translation termination.

Published
2007

4. [Characterization of missense mutations in the SUP45 gene of Saccharomyces cerevisiae encoding translation termination factor eRF1].

Author

Moskalenko SE, Zhuravleva GA, Soom MIa, Shabel'skaia SV, Volkov KV, Zemlianko OM, Philippe M, Mironova LN, and Inge-Vechtomov SG

Subjects
Amino Acid Sequence, Amino Acid Substitution genetics, Molecular Sequence Data, Peptide Termination Factors analysis, Peptide Termination Factors metabolism, Saccharomyces cerevisiae chemistry, Saccharomyces cerevisiae Proteins analysis, Saccharomyces cerevisiae Proteins metabolism, Two-Hybrid System Techniques, Codon, Terminator genetics, Mutation, Missense genetics, Peptide Chain Termination, Translational genetics, Peptide Termination Factors genetics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics
Abstract

Collection of missense mutations in the SUP45 gene of Saccharomyces cerevisiae encoding translation termination factor eRF1 has been obtained by different approaches. It has been shown that most of isolated mutations cause amino acid substitutions in the N-terminal part of eRF1 and do not decrease the eRF1 amount. Most of mutations studied do not abolish eRF1-eRF3 interaction. The role of the N-terminal part of eRF1 in stop codon recognition is discussed.

Published
2004

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