23 results on '"Olsen, Maia"'
Search Results
2. Background 210Po activity concentrations in Greenland marine biota and dose assessment
- Author
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Hansen, Violeta, Mosbech, Anders, Rigét, Frank Farsø, Søgaard-Hansen, Jens, Bjerregaard, Peter, Dietz, Rune, Sonne, Christian, Asmund, Gert, Bøknæs, Niels, Olsen, Maia, Gustavson, Kim, Boertmann, David, Fabricius, Sandra Drewes, Clausen, Daniel Spelling, and Hansen, Alexander Serban
- Published
- 2022
- Full Text
- View/download PDF
3. The Lancet NCDI Poverty Commission: bridging a gap in universal health coverage for the poorest billion
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Adjaye-Gbewonyo, Kafui, Adler, Alma, Amegashie, Fred, Amuyunzu-Nyamongo, Mary Kigasia, Arwal, Said Habib, Bassoff, Nicole, Beste, Jason A., Boudreaux, Chantelle, Byass, Peter, Cadet, Jean Roland, Dagnaw, Wubaye Walelgne, Eagan, Arielle Wilder, Feigl, Andrea, Gathecha, Gladwell, Haakenstad, Annie, Haileamlak, Abraham Mitike, Johansson, Kjell Arne, Kamanda, Mamusu, Karmacharya, Biraj, Kasomekera, Noel, Kintu, Alex, Koirala, Bhagawan, Kwan, Gene F., Larco, Nancy Charles, Maongezi, Sarah, Masiye, Jones, Mayige, Mary, McLaughlin, Amy, Memirie, Solomon Tessema, Muquingue, Humberto Nelson, Mwangi, Kibachio Joseph Muiruri, Ndayisaba, Gilles Francois, Noble, Christopher A., Noormal, Bashir, Olsen, Maia, Park, Paul, Aguilar, Gisela Robles, Sankoh, Osman A., Saxena, Akshar, Schwartz, Leah N., Schwarz, Dan K., Shaffer, Jonathan D., Sumner, Andrew P., Doe, Zoe Taylor, Upreti, Senendra Raj, Verguet, Stéphane, Watkins, David, Wroe, Emily B., Bukhman, Gene, Mocumbi, Ana O, Atun, Rifat, Becker, Anne E, Bhutta, Zulfiqar, Binagwaho, Agnes, Clinton, Chelsea, Coates, Matthew M, Dain, Katie, Ezzati, Majid, Gottlieb, Gary, Gupta, Indrani, Gupta, Neil, Hyder, Adnan A, Jain, Yogesh, Kruk, Margaret E, Makani, Julie, Marx, Andrew, Miranda, J Jaime, Norheim, Ole F, Nugent, Rachel, Roy, Nobhojit, Stefan, Cristina, Wallis, Lee, and Mayosi, Bongani
- Published
- 2020
- Full Text
- View/download PDF
4. WHO framework on meaningful engagement: A transformational approach to integrate lived experience in the noncommunicable disease and mental health agenda
- Author
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Fisher, Jack, primary, Fones, Guy, additional, Arivalagan, Yvonne, additional, Ahmadpour, Ida, additional, Akselrod, Svetlana, additional, and Olsen, Maia, additional
- Published
- 2024
- Full Text
- View/download PDF
5. Realigning noncommunicable disease monitoring with health systems priorities in the Africa region
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Boudreaux, Chantelle, primary, Salvi, Devashri, additional, Adler, Alma J, additional, Wroe, Emily B, additional, Coates, Matthew M, additional, Olsen, Maia, additional, Jain, Yogesh, additional, Mocumbi, Ana O, additional, and Bukhman, Gene, additional
- Published
- 2023
- Full Text
- View/download PDF
6. DNA from keratinous tissue. Part I: Hair and nail
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Bengtsson, Camilla Friis, Olsen, Maia E., Brandt, Luise Ørsted, Bertelsen, Mads F., Willerslev, Eske, Tobin, Desmond J., Wilson, Andrew S., and Gilbert, M. Thomas P.
- Published
- 2012
- Full Text
- View/download PDF
7. DNA from keratinous tissue: Part II: Feather
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Olsen, Maia E., Bengtsson, Camilla Friis, Bertelsen, Mads F., Willerslev, Eske, and Gilbert, M. Thomas P.
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- 2012
- Full Text
- View/download PDF
8. Fossil avian eggshell preserves ancient DNA
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Oskam, Charlotte L., Haile, James, McLay, Emma, Rigby, Paul, Allentoft, Morten E., Olsen, Maia E., Bengtsson, Camilla, Miller, Gifford H., Schwenninger, Jean-Luc, Jacomb, Chris, Walter, Richard, Baynes, Alexander, Dortch, Joe, Parker-Pearson, Michael, Gilbert, M. Thomas P., Holdaway, Richard N., Willerslev, Eske, and Bunce, Michael
- Published
- 2010
9. Hydroclimatic Controls on the Isotopic (δ18 O, δ2 H, d-excess) Traits of Pan-Arctic Summer Rainfall Events
- Author
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Mellat, Moein, primary, Bailey, Hannah, additional, Mustonen, Kaisa-Riikka, additional, Marttila, Hannu, additional, Klein, Eric S., additional, Gribanov, Konstantin, additional, Bret-Harte, M. Syndonia, additional, Chupakov, Artem V., additional, Divine, Dmitry V., additional, Else, Brent, additional, Filippov, Ilya, additional, Hyöky, Valtteri, additional, Jones, Samantha, additional, Kirpotin, Sergey N., additional, Kroon, Aart, additional, Markussen, Helge Tore, additional, Nielsen, Martin, additional, Olsen, Maia, additional, Paavola, Riku, additional, Pokrovsky, Oleg S., additional, Prokushkin, Anatoly, additional, Rasch, Morten, additional, Raundrup, Katrine, additional, Suominen, Otso, additional, Syvänperä, Ilkka, additional, Vignisson, Sölvi Rúnar, additional, Zarov, Evgeny, additional, and Welker, Jeffrey M., additional
- Published
- 2021
- Full Text
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10. The Lancet NCDI Poverty Commission: bridging a gap in universal health coverage for the poorest billion
- Author
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Bukhman, Gene, primary, Mocumbi, Ana O, additional, Atun, Rifat, additional, Becker, Anne E, additional, Bhutta, Zulfiqar, additional, Binagwaho, Agnes, additional, Clinton, Chelsea, additional, Coates, Matthew M, additional, Dain, Katie, additional, Ezzati, Majid, additional, Gottlieb, Gary, additional, Gupta, Indrani, additional, Gupta, Neil, additional, Hyder, Adnan A, additional, Jain, Yogesh, additional, Kruk, Margaret E, additional, Makani, Julie, additional, Marx, Andrew, additional, Miranda, J Jaime, additional, Norheim, Ole F, additional, Nugent, Rachel, additional, Roy, Nobhojit, additional, Stefan, Cristina, additional, Wallis, Lee, additional, Mayosi, Bongani, additional, Adjaye-Gbewonyo, Kafui, additional, Adler, Alma, additional, Amegashie, Fred, additional, Amuyunzu-Nyamongo, Mary Kigasia, additional, Arwal, Said Habib, additional, Bassoff, Nicole, additional, Beste, Jason A., additional, Boudreaux, Chantelle, additional, Byass, Peter, additional, Cadet, Jean Roland, additional, Dagnaw, Wubaye Walelgne, additional, Eagan, Arielle Wilder, additional, Feigl, Andrea, additional, Gathecha, Gladwell, additional, Haakenstad, Annie, additional, Haileamlak, Abraham Mitike, additional, Johansson, Kjell Arne, additional, Kamanda, Mamusu, additional, Karmacharya, Biraj, additional, Kasomekera, Noel, additional, Kintu, Alex, additional, Koirala, Bhagawan, additional, Kwan, Gene F., additional, Larco, Nancy Charles, additional, Maongezi, Sarah, additional, Masiye, Jones, additional, Mayige, Mary, additional, McLaughlin, Amy, additional, Memirie, Solomon Tessema, additional, Muquingue, Humberto Nelson, additional, Mwangi, Kibachio Joseph Muiruri, additional, Ndayisaba, Gilles Francois, additional, Noble, Christopher A., additional, Noormal, Bashir, additional, Olsen, Maia, additional, Park, Paul, additional, Aguilar, Gisela Robles, additional, Sankoh, Osman A., additional, Saxena, Akshar, additional, Schwartz, Leah N., additional, Schwarz, Dan K., additional, Shaffer, Jonathan D., additional, Sumner, Andrew P., additional, Doe, Zoe Taylor, additional, Upreti, Senendra Raj, additional, Verguet, Stéphane, additional, Watkins, David, additional, and Wroe, Emily B., additional
- Published
- 2020
- Full Text
- View/download PDF
11. Parasitoids indicate major climate‐induced shifts in arctic communities
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Kankaanpää, Tuomas, primary, Vesterinen, Eero, additional, Hardwick, Bess, additional, Schmidt, Niels M., additional, Andersson, Tommi, additional, Aspholm, Paul E., additional, Barrio, Isabel C., additional, Beckers, Niklas, additional, Bêty, Joël, additional, Birkemoe, Tone, additional, DeSiervo, Melissa, additional, Drotos, Katherine H. I., additional, Ehrich, Dorothee, additional, Gilg, Olivier, additional, Gilg, Vladimir, additional, Hein, Nils, additional, Høye, Toke T., additional, Jakobsen, Kristian M., additional, Jodouin, Camille, additional, Jorna, Jesse, additional, Kozlov, Mikhail V., additional, Kresse, Jean‐Claude, additional, Leandri‐Breton, Don‐Jean, additional, Lecomte, Nicolas, additional, Loonen, Maarten, additional, Marr, Philipp, additional, Monckton, Spencer K., additional, Olsen, Maia, additional, Otis, Josée‐Anne, additional, Pyle, Michelle, additional, Roos, Ruben E., additional, Raundrup, Katrine, additional, Rozhkova, Daria, additional, Sabard, Brigitte, additional, Sokolov, Aleksandr, additional, Sokolova, Natalia, additional, Solecki, Anna M., additional, Urbanowicz, Christine, additional, Villeneuve, Catherine, additional, Vyguzova, Evgenya, additional, Zverev, Vitali, additional, and Roslin, Tomas, additional
- Published
- 2020
- Full Text
- View/download PDF
12. Assessing and Addressing the Need for Cancer Patient Education in a Resource-Limited Setting in Haiti
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Schleimer, Lauren E., primary, Desameau, Peter-Gens, additional, Damuse, Ruth, additional, Olsen, Maia, additional, Manzo, Veronica, additional, Cardenas, Carlos, additional, Mehrtash, Hedieh, additional, Krakauer, Eric L., additional, Masamba, Leo, additional, Wang, Catharine, additional, Bhatt, Ami S., additional, Shulman, Lawrence N., additional, and Huang, Franklin W., additional
- Published
- 2020
- Full Text
- View/download PDF
13. Background 210Po activity concentrations in Greenlandic seafood and marine mammals, and dose assessment
- Author
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Hansen, Violeta, Mosbech, Anders, Riget, Frank Farsø, Asmund, Gert, Bøknæs, Niels, Bjerregaard, Peter, Olsen, Maia, Gustavson, Kim, Dietz, Rune, Sonne, Christian, Fabricius, Sandra Drewes, and Søgaard-Hansen , Jens
- Published
- 2020
14. BioBasis manual:Conceptual design and sampling procedures of the biological monitoring programme within NuukBasic. Nuuk Ecological Research Operations
- Author
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Raundrup, Katrine, Olsen, Maia, Jacobsen, Ida B. Dyrholm, Nymand, Josephine, Lauridsen, Torben, Krogh, Paul Henning, Schmidt, Niels Martin, Illeris, Lotte, Ro-Poulsen, Helge, and Aastrup, Peter
- Abstract
The NuukBasic programme was initiated in 2007 by the National Environmental Research Institute, Aarhus University, in cooperation with the Greenland Institute of Natural Resources. NuukBasic is funded by the Danish Energy Agency and the Danish Environmental Protection Agency as part of the environmental support programme DANCEA – Danish Cooperation for Environment in the Arctic. The present manual describes methods and sampling procedures of the subprogram BioBasis. The manual is updated regularly. The latest version can always be found at www.nuuk-basic.dk.NuukBasic is a climate change effects monitoring programme with the monitoring sites located close to Nuuk in west Greenland. The programme studies the effects of climate variability and change on marine and terrestrial ecosystems. In terms of scientific concept, NuukBasic is equivalent to the investigations carried out in ZackenbergBasic, at Zackenberg Research Station in Northeast Greenland (www.zackenberg.dk).The terrestrial monitoring area is located at the inner parts of Kobbefjord approximately 20 km east of Nuuk as seen in figure 1 and 2. The local climate is low arctic with a mean annual temperature of - 0.1°C (2008-2017). The warmest month is July (average 10.6°C) while the average temperature in March is - 8.2°C (coldest month). The annual mean precipitation in Kobbefjord is around 800 mm and maximum snow depths vary strongly between 0.3 and 1.3 m at the climate station. The drainage basin is located in an alpine landscape with mountains rising up to 1400 meter above sea level and with glacier coverage of approximately 2km2. Geologically, the area is relatively homogenous with precambrium gneisses as basement throughout the drainage basin. For a presentation of the Kobbefjord geology please refer to the note on “Kobbefjord geology” by Thebaud and Lebrun which can be found in the cabin in Kobbefjord.
- Published
- 2019
15. The LancetNCDI Poverty Commission: bridging a gap in universal health coverage for the poorest billion
- Author
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Bukhman, Gene, Mocumbi, Ana O, Atun, Rifat, Becker, Anne E, Bhutta, Zulfiqar, Binagwaho, Agnes, Clinton, Chelsea, Coates, Matthew M, Dain, Katie, Ezzati, Majid, Gottlieb, Gary, Gupta, Indrani, Gupta, Neil, Hyder, Adnan A, Jain, Yogesh, Kruk, Margaret E, Makani, Julie, Marx, Andrew, Miranda, J Jaime, Norheim, Ole F, Nugent, Rachel, Roy, Nobhojit, Stefan, Cristina, Wallis, Lee, Mayosi, Bongani, Adjaye-Gbewonyo, Kafui, Adler, Alma, Amegashie, Fred, Amuyunzu-Nyamongo, Mary Kigasia, Arwal, Said Habib, Bassoff, Nicole, Beste, Jason A., Boudreaux, Chantelle, Byass, Peter, Cadet, Jean Roland, Dagnaw, Wubaye Walelgne, Eagan, Arielle Wilder, Feigl, Andrea, Gathecha, Gladwell, Haakenstad, Annie, Haileamlak, Abraham Mitike, Johansson, Kjell Arne, Kamanda, Mamusu, Karmacharya, Biraj, Kasomekera, Noel, Kintu, Alex, Koirala, Bhagawan, Kwan, Gene F., Larco, Nancy Charles, Maongezi, Sarah, Masiye, Jones, Mayige, Mary, McLaughlin, Amy, Memirie, Solomon Tessema, Muquingue, Humberto Nelson, Mwangi, Kibachio Joseph Muiruri, Ndayisaba, Gilles Francois, Noble, Christopher A., Noormal, Bashir, Olsen, Maia, Park, Paul, Aguilar, Gisela Robles, Sankoh, Osman A., Saxena, Akshar, Schwartz, Leah N., Schwarz, Dan K., Shaffer, Jonathan D., Sumner, Andrew P., Doe, Zoe Taylor, Upreti, Senendra Raj, Verguet, Stéphane, Watkins, David, and Wroe, Emily B.
- Published
- 2020
- Full Text
- View/download PDF
16. Conceptual design and sampling procedures of the biological programme of NuukBasic
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Aastrup, Peter, Nymand, Josephine, Raundrup, Katrine, Olsen, Maia, Lauridsen, Torben Linding, Krogh, Paul Henning, Schmidt, Niels Martin, Illeris, Lotte, and Ro-Poulsen, Helge
- Subjects
carbon flux ,microarthropods ,monitoring ,NDVI ,UV-B ,arctic ,lake ecology ,arthropods ,phenology - Abstract
This manual describes procedures for biologic climate eff ectmonitoring in Kobbefjord, Nuuk. The monitoring is a part ofNuukBasic which is a cross-disciplinary ecological monitoringprogramme in low Arctic West Greenland. Biologicalmonitoring comprises the NERO line which is a permanentvegetation transect, and monitoring reproductive phenologyof Salix glauca, Loiseleuria procumbens, Eriophorumangustifolium, and Silene acaulis. The progression in vegetationgreenness is followed along the vegetation transectand in the plant phenology plots by measurement of NormalizedDiff erence Vegetation Index (NDVI). The fl ux of CO2is measured in natural conditions as well as in manipulationssimulating increased temperature, increased cloud cover,shorter growing season, and longer growing season. The effectof increased UV-B radiation on plant stress is studied bymeasuring chlorophyll fl uorescence in three series of plots.Arthropods are sampled by means of yellow pitfall traps aswell as in window traps. Microarthropods are sampled in soilcores and extracted in an extractor by gradually heatingup soil. The avifauna is monitored with special emphasison passerine birds. Only few terrestrial mammals occur inthe study area. All observations of mammals are recordedad-hoc. Monitoring in lakes include ice cover, water chemistry,physical conditions, species composition of plankton,vegetation, bottom organisms and fi sh. Physical-chemicalparameters, phytoplankton and zooplankton
- Published
- 2015
17. Global Design Strategy for Cancer Patient Education Materials: Haiti Pilot Case Study
- Author
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Cardenas, Carlos, primary, Schleimer, Lauren E., additional, Olsen, Maia, additional, Manzo, Veronica, additional, Guay, Rachael, additional, Kim, Taerim, additional, Desameau, Peter-Gens, additional, Damuse, Ruth, additional, Shulman, Lawrence N., additional, Huang, Franklin W., additional, and Bhatt, Ami S., additional
- Published
- 2016
- Full Text
- View/download PDF
18. Developing and Evaluating Culturally and Literacy Appropriate Cancer Patient Education Materials for Haiti
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Schleimer, Lauren E., primary, Desameau, Peter-Gens, additional, Damuse, Ruth, additional, Olsen, Maia, additional, Manzo, Veronica, additional, Guay, Rachael, additional, Bhatt, Ami S., additional, Cardenas, Carlos, additional, Huang, Franklin W., additional, and Shulman, Lawrence N., additional
- Published
- 2016
- Full Text
- View/download PDF
19. Nuuk Basic:BioBasis programme
- Author
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Olsen, Maia, Nymand, Josephine, Raundrup, Katrine, Aastrup, Peter, Krogh, Paul Henning, Lauridsen, Torben Linding, Lund, Magnus, Albert, Kristian Rost, Magelund Jensen, Lillian, and Christensen, Torben Røjle
- Abstract
Results of the seventh year of the BioBasis monitoring programme at Nuuk arepresented. Reproductive phenology: In L. procumbens and S. acaulis vegetative phenology is similar to that of 2012, although there was a tendency to produce larger numbers of flowers in most plots. The timing of 50 % flowering was within the same range as previous years. The flower bud production, the onset of flowering, and the peak flower production of L. procumbens occurred at approximately the same timeas in 2012. A second bud production was recorded 3 September Day of Year (DOY) 246. All plots produced more flowers in 2013 than in 2012. The first senescent flowers were recorded about the same time as in 2012. Timing of budding, flowering and senescence in S. acaulis was later thanin 2012. S. glauca buds were observed aweek later compared to the earliest year 2010. First flowering male catkins were observed 13 June (DOY 164) and first female catkins 18 June (DOY 169). The first female flowers with hair were observed 11 July. A higher number of flowers were produced in 2013 than in 2012. During April the area was exposed to two weeks of severe snowmelt, followed by heavy snowfall in May, resulting in late snowmelt. A preliminary review of data related to flowering indicates that 2013 was characterized by no larval outbreak of the noctuid moth E. occulta, and large numbers of flowers produced in L. procumbens and S. glauca compared to 2012. Vegetation greening: Empetrum nigrum reached the highest Normalised Difference Vegetation Index (NDVI) values of all the species, and NDVI was consistently high throughout the season. The values were at the same level or higher than in 2012, which was the highest recorded so far. Generally, the vegetation greening in 2013 was very similar to that in 2012. The NDVI values were in many cases higher throughout the season compared to previous years, with an intermediate to late peak. The vegetation seems to have recovered after the outbreak of the noctuid moth larvae.Carbon dioxide exchange: Since 2008 carbon dioxide exchange has been measured including manipulation experiments simulating higher temperatures and increased cloud cover. Generally, all plots functioned as sinks for atmospheric CO2 at the time of the measurement (midday). In October, Net Ecosystem Exchange (NEE) was generally close to zero. Similar to previous years the uptake of CO2 was higher in control plots compared to plots with elevated temperature and shaded plots. The differences between treatments during 2013 were in general similar to previous years. Together with 2012, the flux amplitudes in 2013 were higher compared with previous years. UV-B exclusion plots: Measurements of chlorophyll fluorescence as a proxy forplant stress were carried out. The total performance index (PItotal) integrates the measurements, and the PItotal were sensitive to UV-B exclusion in both V.uliginosum and B. nana. The seasonal plant stress level was reduced when the plants were protected from UV-B radiation.Arthropods: Pitfall traps were established from 28 May (DOY 148) through 13 June (DOY 164) and they all worked continuously until 7 October (DOY 280) when the liquid began to freeze. In 2013, arthropods were caught during 4511 trapdays (including 4057 pitfall-trap daysand 454 window-trap days). Parts of the samples are being sorted at Department of Bioscience, Aarhus University, Denmark. The material sampled in the 2013 season is currently stored in 70 % ethanol at Greenland Institute of Natural Resources. Microarthropods: In the season 2013, the sampling of microarthropods was reduced from the standard of eight samples per plot to four samples per plot. Sampling was carried out three times during the season. The samples have not been analysed yet. The material sampled and extracted is currently stored in 70 %ethanol at the Greenland Institute of Natural Resources. Materials collected from research projects completed in 2012 in Kobbefjord and Zackenberg were further analysed. For project description andresults see chapter 6.Birds: No survey of breeding passerines was carried out in 2013. A breeding pair of white-tailed eagles Haliaeetus albicilla was observed on the mountain side northeast of the research house in Kobbefjord. All four species of passerines (Lapland buntings, snow buntings, northern wheatears, and redpolls) were already present at the time of the first census, and the survey was carried out until no more observations were made at any census point. The total number of passerines has varied between the years. In 2013, the northern wheatear was the most abundantly observed passerine, and the only species to have more observations per census in 2013 than in 2012. Although there is some variance from year to year, the number of observations of northern wheatears is usually at its maximum in August. Mammals: The Kobbefjord catchment area is only sparsely populated with mammals. In 2013, at least two different foxes have been observed on several occasions; tracks, faeces and barks have been observed/heard throughout the season. One Arctic hare was observed. Lakes: 2013 was relatively wet, particularly during the winter period. It was the coldest summer registered during the monitored period and the ice-free period lasted less than 132 days in Badesø and less than 124 in Qassi-sø. Average total nitrogen concentration in both lakes was the lowest measured and average totalphosphorus was generally low in Badesø. During the last three years, chlorophylla has stabilized around 0.8 μg Chl a l-1, following an increasing trend during the2008-2010 period. Zooplankton biomass is generally higher in Qassi-sø compared to Badesø, which is consistent with the lack of fi sh in Qassi-sø. During the past three years an increasing zooplankton biomass is observed in Badesø; this can partly be due to reduced predation pressure, corresponding well with reduced fi sh abundance in 2013 compared to 2008, when the first fish investigation was undertaken.
- Published
- 2014
20. Environmental monitoring at the Nalunaq Gold Mine, South Greenland, 2013
- Author
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Bach, Lis, Olsen, Maia, and Asmund, Gert
- Subjects
monitoring ,blue mussel ,Greenland ,Nalunaq Gold Mine ,metals ,brown seaweed ,shorthorn sculpin ,Arctic char ,lichen ,environment ,transplantation - Published
- 2014
21. DNA from keratinous tissue. Part II:feather
- Author
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Olsen, Maia E., Bengtsson, Camilla Friis, Bertelsen, Mads Frost, Willerslev, Eske, Gilbert, Tom, Olsen, Maia E., Bengtsson, Camilla Friis, Bertelsen, Mads Frost, Willerslev, Eske, and Gilbert, Tom
- Abstract
Although good quality DNA can be recovered from the base of the calamus of freshly sampled feathers, as from other fully keratinized tissues such as nail or hair shaft, the quality and quantity of DNA in the majority of feather structures is much poorer. Little research has been performed to characterize the quality of this DNA is, and thus what a researcher might be able to achieve when using feathers as a source of DNA. In this review, we expand on our companion article detailing the quality of DNA in nail and hair, by synthesizing published, and new preliminary genetic data obtained from feathers. As with nail and hair, we demonstrate that although DNA can, in general, be recovered from all parts of the feather, the quality of such DNA varies. As such, although one can expect a priori that genetic analyses are possible on the feather, for PCR based analyses, it is extremely difficult to predict the size of amplicon that can be used in such analyses. However, PCR-free genetic analyses that can exploit much smaller DNA fragments may promise to be a powerful tool for future exploitation.
- Published
- 2012
22. DNA from keratinous tissue. Part I:hair and nail
- Author
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Bengtsson, Camilla Friis, Olsen, Maia E., Brandt, Luise Ørsted, Bertelsen, Mads Frost, Willerslev, Eske, Tobin, Desmond J., Wilson, Andrew S., Gilbert, Tom, Bengtsson, Camilla Friis, Olsen, Maia E., Brandt, Luise Ørsted, Bertelsen, Mads Frost, Willerslev, Eske, Tobin, Desmond J., Wilson, Andrew S., and Gilbert, Tom
- Abstract
Keratinous tissues such as nail, hair, horn, scales and feather have been used as a source of DNA for over 20 years. Particular benefits of such tissues include the ease with which they can be sampled, the relative stability of DNA in such tissues once sampled, and, in the context of ancient genetic analyses, the fact that sampling generally causes minimal visual damage to valuable specimens. Even when freshly sampled, however, the DNA quantity and quality in the fully keratinized parts of such tissues is extremely poor in comparison to other tissues such as blood and muscle - although little systematic research has been undertaken to characterize how such degradation may relate to sample source. In this review paper we present the current understanding of the quality and limitations of DNA in two key keratinous tissues, nail and hair. The findings indicate that although some fragments of nuclear and mitochondrial DNA appear to be present in almost all hair and nail samples, the quality of DNA, both in quantity and length of amplifiable DNA fragments, vary considerably not just by species, but by individual, and even within individual between hair types.
- Published
- 2012
23. Fossil avian eggshell preserves ancient DNA
- Author
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Oskam, Charlotte L, Haile, James Seymour, McLay, Emma, Rigby, Paul, Allentoft, Morten Erik, Olsen, Maia E., Bengtsson, Camilla, Miller, Gifford H., Schwenninger, Jean-Luc, Jacomb, Christopher, Walter, Richard, Baynes, Alexander, Dortch, Joe, Parker-Pearson, Michael, Gilbert, Tom, Holdaway, Richard N., Willerslev, Eske, Bunce, Michael, Oskam, Charlotte L, Haile, James Seymour, McLay, Emma, Rigby, Paul, Allentoft, Morten Erik, Olsen, Maia E., Bengtsson, Camilla, Miller, Gifford H., Schwenninger, Jean-Luc, Jacomb, Christopher, Walter, Richard, Baynes, Alexander, Dortch, Joe, Parker-Pearson, Michael, Gilbert, Tom, Holdaway, Richard N., Willerslev, Eske, and Bunce, Michael
- Abstract
Owing to exceptional biomolecule preservation, fossil avian eggshell has been used extensively in geochronology and palaeodietary studies. Here, we show, to our knowledge, for the first time that fossil eggshell is a previously unrecognized source of ancient DNA (aDNA). We describe the successful isolation and amplification of DNA from fossil eggshell up to 19 ka old. aDNA was successfully characterized from eggshell obtained from New Zealand (extinct moa and ducks), Madagascar (extinct elephant birds) and Australia (emu and owl). Our data demonstrate excellent preservation of the nucleic acids, evidenced by retrieval of both mitochondrial and nuclear DNA from many of the samples. Using confocal microscopy and quantitative PCR, this study critically evaluates approaches to maximize DNA recovery from powdered eggshell. Our quantitative PCR experiments also demonstrate that moa eggshell has approximately 125 times lower bacterial load than bone, making it a highly suitable substrate for high-throughput sequencing approaches. Importantly, the preservation of DNA in Pleistocene eggshell from Australia and Holocene deposits from Madagascar indicates that eggshell is an excellent substrate for the long-term preservation of DNA in warmer climates. The successful recovery of DNA from this substrate has implications in a number of scientific disciplines; most notably archaeology and palaeontology, where genotypes and/or DNA-based species identifications can add significantly to our understanding of diets, environments, past biodiversity and evolutionary processes.
- Published
- 2010
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