1. Anion protection of CuZnSOD during peroxidative activity with H(2)O(2).
- Author
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Jewett SL, Olmsted HK, Marach JA, Rojas F, and Silva K
- Subjects
- Animals, Binding Sites, Carbon chemistry, Carbonates chemistry, Cattle, Cyclic N-Oxides chemistry, Free Radicals, Hydrogen Peroxide chemistry, Hydrogen-Ion Concentration, Liver enzymology, Models, Chemical, Spectrophotometry, Superoxide Dismutase chemistry, Time Factors, Anions, Hydrogen Peroxide metabolism, Superoxide Dismutase metabolism
- Abstract
The "peroxidase" activity of the copper-zinc superoxide dismutase is a poorly sustained activity because of the competing inactivation of the enzyme. New evidence suggests that the bound oxidant may be partitioning between oxidizing the enzyme or oxidizing small anions. At constant peroxide, nitrite and azide only partially protect the enzyme (50%) against loss of copper(I) and inactivation up to one anion per copper. Beyond that level, there is no further protection. Bicarbonate ion also protects, but larger amounts are required. These data suggest that there is significant oxidation of the enzyme even in the presence of the small anions and therefore the formation of the bound oxidant cannot be sustained in a true catalytic process., (Copyright 2000 Academic Press.)
- Published
- 2000
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