11 results on '"Olivier, Jessica"'
Search Results
2. Detection of cardiac apoptosis by [18F]ML-10 in a mouse model of permanent LAD ligation
- Author
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Fischer, Maximilian, Olivier, Jessica, Lindner, Simon, Zacherl, Mathias J., Massberg, Steffen, Bartenstein, Peter, Ziegler, Sibylle, Brendel, Matthias, Lehner, Sebastian, Boening, Guido, and Todica, Andrei
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- 2022
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3. Myxosarkom in der Halsregion eines Teddy-Hamsters (Mesocricetus auratus)
- Author
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Olivier, Jessica, additional, Weiler, Kristina, additional, Hartung, Svenja, additional, Weyrich, Angelika, additional, and Bauer, Natali, additional
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- 2023
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4. Detection of apoptosis by [18F]ML-10 after cardiac ischemia–reperfusion injury in mice
- Author
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Fischer, Maximilian, primary, Zacherl, Mathias J., additional, Olivier, Jessica, additional, Lindner, Simon, additional, Massberg, Steffen, additional, Bartenstein, Peter, additional, Grawe, Freba, additional, Ziegler, Sibylle, additional, Brendel, Matthias, additional, Lehner, Sebastian, additional, Boening, Guido, additional, and Todica, Andrei, additional
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- 2022
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5. Detection of apoptosis by [18F]ML-10 after cardiac ischemia–reperfusion injury in mice.
- Author
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Fischer, Maximilian, Zacherl, Mathias J., Olivier, Jessica, Lindner, Simon, Massberg, Steffen, Bartenstein, Peter, Grawe, Freba, Ziegler, Sibylle, Brendel, Matthias, Lehner, Sebastian, Boening, Guido, and Todica, Andrei
- Abstract
Objective: Myocardial infarction leads to ischemic heart disease and cell death, which is still a major obstacle in western society. In vivo imaging of apoptosis, a defined cascade of cell death, could identify myocardial tissue at risk. Methods: Using 2-(5-[
18 F]fluoropentyl)-2-methyl-malonic acid ([18 F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in a mouse model of transient ligation of the left anterior descending (LAD) artery. 2-deoxy-2-[18F]fluoro-D-glucose ([18 F]FDG) PET imaging indicated the defect area. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) histology stain indicated cardiac apoptosis. Results: [18 F]ML-10 uptake was evident in the ischemic area after transient LAD ligation in ex vivo autoradiography and in vivo PET imaging. Detection of [18 F]ML-10 is in line with the defect visualized by [18 F]FDG and the histological approach of TUNEL staining. Conclusion: The tracer [18 F]ML-10 is suitable for detecting apoptosis after transient LAD ligation in mice. [ABSTRACT FROM AUTHOR]- Published
- 2023
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6. Detection of cardiac apoptosis by [18F]ML-10 in a mouse model of permanent LAD ligation.
- Author
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Fischer, Maximilian, Olivier, Jessica, Lindner, Simon, Zacherl, Mathias J., Massberg, Steffen, Bartenstein, Peter, Ziegler, Sibylle, Brendel, Matthias, Lehner, Sebastian, Boening, Guido, and Todica, Andrei
- Abstract
Purpose: The loss of viable cardiac cells and cell death by myocardial infarction (MI) is still a significant obstacle in preventing deteriorating heart failure. Imaging of apoptosis, a defined cascade to cell death, could identify areas at risk. Procedures: Using 2-(5-[18F]fluoropentyl)-2-methyl-malonic acid ([18F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in murine hearts after permanent ligation of the left anterior descending artery (LAD) inducing myocardial infarction (MI). 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) PET imaging localized the infarct area after MI. Histology by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining validated apoptosis in the heart. Results: Accumulation of [18F]ML-10 was evident in the infarct area after permanent ligation of the LAD in autoradiography and PET imaging. Detection of apoptosis by [18F]ML-10 is in line with the defect visualized by [18F]FDG and the histological approach. Conclusion: [18F]ML-10 could be a suitable tracer for apoptosis imaging in a mouse model of permanent LAD ligation. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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7. Molekulare Bildgebung mittels [18F]-ML10 zur Beurteilung des zeitlichen Verlaufs der kardialen Apoptose im Ischämie-Modell der Maus
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Olivier, Jessica
- Subjects
FOS: Veterinary science ,Apoptose, Myokardinfarkt, Ischämie-Modell, FDG, ML10, Autoradiographie, PET, Tracer - Abstract
In der vorliegenden Arbeit wurde der Tracer [18F]-ML10 zur Bestimmung der Apoptose in kardiologischen Ischämie-Modellen (permanent und transient) der Maus zu verschiedenen Zeitpunkten nach Myokardinfarkt untersucht. Es wurden sowohl autoradiographische Untersuchungen als auch µPET-Messung durchgeführt, um die Tracerverteilung und die Akkumulation im infarzierten Myokard zu untersuchen. In beiden Modalitäten reicherte sich [18F]-ML10 im Versorgungsgebiet des Ramus interventricularis anterior nach induziertem Myokardinfarkt an. In der µPET-Messung war die höchste Anreicherung mittels [18F]-FDG im Bereich des dargestellten Infarktareals zu beobachten. Im Gegensatz hierzu war eine Anreicherung in der durchgeführten µPET-Messung im nicht-infarzierten Remote-Myokard nahezu fehlend und in der Autoradiographie zeigte sich ein hohes target-to-background Verhältnis (Infarkt zu Remotemyokard). Die höchste Anreicherung von [18F]-ML10 war sowohl im Modell der permanenten als auch in dem der transienten Ischämie zum frühen Zeitpunkt (2 Stunden) nach Myokardinfarkt in beiden Untersuchungsmethoden zu beobachten. Es fand sich im Modell der transienten Ischämie zum Zeitpunkt 2 Stunden ebenfalls der größte Effekt der Signaländerung und eine signifikant höhere Anreicherung im Vergleich zu späteren Zeitpunkten. Dieses Modell entspricht bereits einer therapeutischen Intervention nach akutem Myokardinfarkt was sich auch in den hohen Variationskoeffizienten bis zum Zeitpunkt 6 Stunden widerspiegelt. Erst zu den späten Zeitpunkten nahm dieser wieder ab. In einem Modell mit hohen Variationskoeffizienten werden vornehmlich größere Effekte durch Interventionstherapien messbar sein. Im Modell der permanenten Ischämie zeichnete sich der Zeitpunkt 6 Stunden sowohl in der Autoradiographie als auch in der µPET-Auswertung mit einem großen Effekt aus und zeigte zusätzlich ein signifikant höheres Signal im Vergleich zu den späteren Zeitpunkten. Auch wenn die Anreicherung zu den früheren Zeitpunkten größer war, zeigte sich der Zeitpunkt 6 Stunden möglicherweise stabiler und für zukünftige Interventionsstudien auf Grund des geringeren Variationskoeffizienten als besser geeignet. Es ergibt sich in diesem Modell zu diesem Zeitpunkt am ehesten eine stabile Vergleichsgruppe für Interventionsstudien in der wahrscheinlich auch kleine Therapieeffekte messbar werden. Allgemein sollte der Zeitpunkt nach Myokardinfarkt und das Ischämie-Modell in Abhängigkeit des zu untersuchenden Zielparameters sorgfältig ausgewählt werden. Zudem sollte der Messzeitpunkt zwischen Vergleichsgruppe und Interventionsgruppe exakt übereinstimmen da diese Studie gezeigt hat, dass innerhalb kurzer Zeitdifferenzen große Effekte in der Signaländerung in beiden Ischämie-Modellen zustande kommen. Zusammenfassend lässt sich festhalten, dass [18F]-ML10 zur spezifischen Darstellung der kardialen Apoptose nach Myokardinfarkt geeignet erscheint. Der höchste Uptake ist frühzeitig nach Infarktentstehung abzugrenzen wobei der Zeitpunkt 6 Stunden nach Ischämie sich auf Grund der weitgehend stabilsten Anreicherung für weitere Therapiemonitoringstudien eignet., In the present study the tracer [18F]-ML10 was used to assess the degree of apoptosis in mouse models of cardiac ischemia (permanent and transient) at various time points after myocardial infarction. Autoradiography and μPET-Imaging were performed to investigate the tracer distribution and accumulation in the infarcted myocardium. [18F]-ML10 was found in both modalities in the supply area of the Ramus interventricularis anterior and therefore in the area of myocardial infarction. In the μPET images tracer accumulation was found too in the area of myocardial infarction visualized by [18F]-FDG PET. In contrast, tracer accumulation in non-infarcted remote myocardium was nearly absent in the μPET scan with a high target-to-background ratio (infarct-to-remote myocardium) in the Autoradiography. The highest [18F]-ML10-uptake in both modalities was during the earliest measured time point approx. 2 hours after myocardial infarction in both evaluated ischemia models. The highest effect in the model of transient ischemia, as well as a significant accumulation of [18F]-ML10 in comparison to the late time points, was seen at the early time point (2 hours). This model represents itself as a therapeutic intervention after myocardial infarction which might have resulted in an increased coefficient of variation at the time point of 6 hours. In this model, due to a high coefficient of variation, effects in intervention-therapy will have to be large to be detected as a significant difference. In both methods (autoradiography and µPET) the time point of 6 hours showed a high effect in the model of permanent ischemia supported by a significantly higher signal as compared to later time points. Although earlier time points showed a higher accumulation this time point might be more stable for further investigations due to the lower coefficient of variation. In this model effects of an interventions-therapy are more likely to be detected even if they are small. The choice of the cardiac ischemia model and the time point following infarction should be chosen carefully based on the specific aim of the planned investigation. Furthermore, the time point of measurement in the intervention- and the control-group should be matched as closely as possible, since the tracer accumulation in the infarcted area shows a high variability during a short period of time in both models. In conclusion, in this study [18F] -ML10 appears to be a suitable tracer in the first in-vivo method for imaging apoptosis following myocardial infarction with the highest uptake early after infarction and a quite stable tracer accumulation approximately 6 hours after ischemia.
- Published
- 2019
- Full Text
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8. 18F–FDG-PET/CT and diffusion-weighted MRI for monitoring a BRAF and CDK 4/6 inhibitor combination therapy in a murine model of human melanoma
- Author
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Eschbach, Ralf S., primary, Kazmierczak, Philipp M., additional, Heimer, Maurice M., additional, Todica, Andrei, additional, Hirner-Eppeneder, Heidrun, additional, Schneider, Moritz J., additional, Keinrath, Georg, additional, Solyanik, Olga, additional, Olivier, Jessica, additional, Kunz, Wolfgang G., additional, Reiser, Maximilian F., additional, Bartenstein, Peter, additional, Ricke, Jens, additional, and Cyran, Clemens C., additional
- Published
- 2018
- Full Text
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9. 18F-FDG-PET/CT and diffusion-weighted MRI for monitoring a BRAF and CDK 4/6 inhibitor combination therapy in a murine model of human melanoma.
- Author
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Eschbach, Ralf S., Kazmierczak, Philipp M., Heimer, Maurice M., Todica, Andrei, Hirner-Eppeneder, Heidrun, Schneider, Moritz J., Keinrath, Georg, Solyanik, Olga, Olivier, Jessica, Kunz, Wolfgang G., Reiser, Maximilian F., Bartenstein, Peter, Ricke, Jens, and Cyran, Clemens C.
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- 2018
- Full Text
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10. [Myxosarcoma in the cervical region in a teddy bear hamster (Mesocricetus auratus)].
- Author
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Olivier J, Weiler K, Hartung S, Weyrich A, and Bauer N
- Subjects
- Animals, Cricetinae, Mesocricetus, Immunohistochemistry, Myxosarcoma diagnosis, Myxosarcoma pathology, Myxosarcoma surgery, Myxosarcoma veterinary, Rodent Diseases
- Abstract
This case report describes a rare case of a myxosarcoma in a 1-year-old teddy bear hamster presenting with a mass in the cervical region. The fine-needle aspiration cytology revealed high numbers of pleomorphic spindle-shaped cells found in a viscous mucinous background. The presumptive cytological diagnosis was malignant spindle cell neoplasia based on marked criteria of malignancy of the mesenchymal cell population. The abundant matrix in the background was suggestive of a myxosarcoma. The hamster died during surgery and a necropsy was performed. Histopathology was in complete agreement with the cytological report. Immunohistochemistry revealed the tumour to be vimentin positive with alcian-blue positive matrix and confirmed the presumptive diagnosis of a myxosarcoma. This case shows that fine-needle aspiration cytology can be utilized as a minimally invasive diagnostic tool in small mammals to classify mass lesions. However, so far little is known about the biological behaviour of myxosarcoma in the hamster as case descriptions are rare., Competing Interests: Die Autoren bestätigen, dass kein Interessenkonflikt besteht., (Thieme. All rights reserved.)
- Published
- 2023
- Full Text
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11. 18 F-FDG-PET/CT and diffusion-weighted MRI for monitoring a BRAF and CDK 4/6 inhibitor combination therapy in a murine model of human melanoma.
- Author
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Eschbach RS, Kazmierczak PM, Heimer MM, Todica A, Hirner-Eppeneder H, Schneider MJ, Keinrath G, Solyanik O, Olivier J, Kunz WG, Reiser MF, Bartenstein P, Ricke J, and Cyran CC
- Subjects
- Aminopyridines administration & dosage, Aminopyridines therapeutic use, Animals, Antineoplastic Agents administration & dosage, Cyclin-Dependent Kinase 4 antagonists & inhibitors, Cyclin-Dependent Kinase 6 antagonists & inhibitors, Female, Imidazoles administration & dosage, Imidazoles therapeutic use, Male, Melanoma drug therapy, Mice, Mice, Inbred BALB C, Mice, Nude, Oximes administration & dosage, Oximes therapeutic use, Protein Kinase Inhibitors administration & dosage, Proto-Oncogene Proteins B-raf antagonists & inhibitors, Purines administration & dosage, Purines therapeutic use, Antineoplastic Agents therapeutic use, Diffusion Magnetic Resonance Imaging methods, Fluorodeoxyglucose F18 pharmacokinetics, Melanoma diagnostic imaging, Positron Emission Tomography Computed Tomography methods, Protein Kinase Inhibitors therapeutic use, Radiopharmaceuticals pharmacokinetics
- Abstract
Background: The purpose of the study was to investigate a novel BRAF and CDK 4/6 inhibitor combination therapy in a murine model of BRAF-V600-mutant human melanoma monitored by
18 F-FDG-PET/CT and diffusion-weighted MRI (DW-MRI)., Methods: Human BRAF-V600-mutant melanoma (A375) xenograft-bearing balb/c nude mice (n = 21) were imaged by18 F-FDG-PET/CT and DW-MRI before (day 0) and after (day 7) a 1-week BRAF and CDK 4/6 inhibitor combination therapy (n = 12; dabrafenib, 20 mg/kg/d; ribociclib, 100 mg/kg/d) or placebo (n = 9). Animals were scanned on a small animal PET after intravenous administration of 20 MBq18 F-FDG. Tumor glucose uptake was calculated as the tumor-to-liver-ratio (TTL). Unenhanced CT data sets were subsequently acquired for anatomic coregistration. Tumor diffusivity was assessed by DW-MRI using the apparent diffusion coefficient (ADC). Anti-tumor therapy effects were assessed by ex vivo immunohistochemistry for validation purposes (microvascular density - CD31; tumor cell proliferation - Ki-67)., Results: Tumor glucose uptake was significantly suppressed under therapy (∆TTLTherapy - 1.00 ± 0.53 vs. ∆TTLControl 0.85 ± 1.21; p < 0.001). In addition, tumor diffusivity was significantly elevated following the BRAF and CDK 4/6 inhibitor combination therapy (∆ADCTherapy 0.12 ± 0.14 × 10-3 mm2 /s; ∆ADCControl - 0.12 ± 0.06 × 10-3 mm2 /s; p < 0.001). Immunohistochemistry revealed a significant suppression of microvascular density (CD31, 147 ± 48 vs. 287 ± 92; p = 0.001) and proliferation (Ki-67, 3718 ± 998 vs. 5389 ± 1332; p = 0.007) in the therapy compared to the control group., Conclusion: A novel BRAF and CDK 4/6 inhibitor combination therapy exhibited significant anti-angiogenic and anti-proliferative effects in experimental human melanomas, monitored by18 F-FDG-PET/CT and DW-MRI.- Published
- 2018
- Full Text
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