1. A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtype
- Author
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Sarah Rank Rønnow, Jannie Marie Bülow Sand, Line Mærsk Staunstrup, Thomas Bahmer, Michael Wegmann, Lars Lunding, Janette Burgess, Klaus Rabe, Grith Lykke Sorensen, Oliver Fuchs, Erika V. Mutius, Gesine Hansen, Matthias Volkmar Kopp, Morten Karsdal, Diana Julie Leeming, Markus Weckmann, and the ALLIANCE Study Group as part of the German Center of Lung Research (DZL)
- Subjects
Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Asthma is a heterogeneous disease; therefore, biomarkers that can assist in the identification of subtypes and direct therapy are highly desirable. Asthma is a chronic inflammatory disease that leads to changes in the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) degradation causing fragments of type I collagen that is released into circulation. Objective Here, we asked if MMP-generated type I collagen (C1M) was associated with subtypes of asthma. Methods C1M was serologically assessed at baseline in the adult participants of the All Age Asthma study (ALLIANCE) (n = 233), and in The Prospective Epidemiological Risk Factor study (PERF) (n = 283). In addition, C1M was assessed in mice sensitized to ovalbumin (OVA) and challenged with OVA aerosol. C1M was evaluated in mice with and without acute neutrophilic inflammation provoked by poly(cytidylic-inosinic) acid and mice treated with CP17, a peptide inhibiting neutrophil accumulation. Results Serum C1M was significantly increased in asthmatics compared to healthy controls (p = 0.0005). We found the increased C1M levels in asthmatics were related to blood neutrophil and body mass index (BMI) in the ALLIANCE cohort, which was validated in the PERF cohort. When patients were stratified into obese (BMI > 30) asthmatics with high neutrophil levels and uncontrolled asthma, this group had a significant increase in C1M compared to normal-weight (BMI
- Published
- 2022
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