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13 results on '"Oliveira, Isabel B."'

11. Evaluation of female aphally in imposex-affected populations of Nucella lapillus at the southernmost distributional limit of the species in Europe.

Author

Sánchez-Marín, Paula, Oliveira, Isabel B., Sousa, Ana C. A., Shin Takahashi, Shinsuke Tanabe, and Galante-Oliveira, Susana

Subjects
*NUCELLA lapillus, *MOLLUSK populations, *GEOGRAPHICAL distribution of mollusks, *ANIMAL species, *TRIBUTYLTIN
Abstract

Male and female aphally in Nucella lapillus has been reported in some populations from the northeastern Atlantic coast, namely in localized areas in southeastern England, northwestern France and northwestern Spain. This occurrence has been associated with Dumpton syndrome (DS), a genetic anomaly that causes under-development of the male genital tract and confers resistance to the masculinizing effects of tributyltin (TBT), i.e. to the development of imposex (superimposition of male sexual characters on neogastropod females). In an effort to elucidate the potential occurrence of DS among Portuguese populations of N. lapillus, data obtained from imposex levels monitoring campaigns performed from 2000 to 2006, during which female aphally was frequently observed, were compiled and analysed. Several characteristics associated with a demasculinizing syndrome were observed in two sampling sites near the port of Lisbon: high frequency of female aphally, undersized penises in males and lower incidence of TBT-induced imposex together with the simultaneous presence of females with contrasting vas deferens sequence values (0 and 4) at the same site. Despite the similarities with DS, the relatively lower levels of TBT pollution recorded in Portugal in comparison with other European coastal areas, and the fact that male aphally was only occasionally found, are reasons to suggest that other nongenetic causes are potentially involved in the development of female aphally in this geographical area. Nevertheless, a lower sensitivity to TBT of Portuguese N. lapillus populations that present a high frequency of aphally is apparent and this hypothetical resistance is discussed. [ABSTRACT FROM AUTHOR]

Published
2016
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13. Tralopyril bioconcentration and effects on the gill proteome of the Mediterranean mussel Mytilus galloprovincialis

Author

Oliveira, Isabel B., Groh, Ksenia J., Stadnicka-Michalak, Julita, Schonenberger, Rene, Beiras, Ricardo, Barroso, Carlos M., Langford, Katherine H., Thomas, Kevin V., and Suter, Marc J. -F.

Subjects
Tralopyril, animal structures, Mytilus galloprovincialis, Proteomic differential analysis, Antifouling biocides, Multidimensional protein identification technology (MudPIT)
Abstract

Antifouling (AF) systems are used worldwide as one of the most cost-effective ways of protecting submerged structures against heavy biofouling. The emergence of environmentally friendly AF biocides requires knowledge on their environmental fate and toxicity. In this study we measured the bioconcentration of the emerging AF biocide tralopyril (TP) in the Mediterranean mussel Mytilus galloprovincialis and investigated the effects of TP on the mussel gill proteome following acute (2 days) and chronic (30 days) exposure, as well as after a 10-day depuration period. The experiments were carried out with 1 mu g/L TP; blank and solvent (5 x 10(-5)% DMSO) controls were also included. Proteomics analysis was performed by mass spectrometry-based multidimensional protein identification technology (MudPIT). Differentially expressed proteins were identified using a label-free approach based on spectral counts and G-test. Our results show that TP is rapidly accumulated by mussels at concentrations up to 362 ng/g dw (whole tissues), reaching steady-state condition within 13 days. Ten days of depuration resulted in 80% elimination of accumulated TP from the organism, suggesting that a complete elimination could be reached with longer depuration times. In total, 46 proteins were found to be regulated in the different exposure scenarios. Interestingly, not only TP but also DMSO alone significantly modulated the protein expression in mussel gills following acute and chronic exposure. Both compounds regulated proteins involved in bioenergetics, immune system, active efflux and oxidative stress, often in the opposite way. Alterations of several proteins, notably several cytoskeletal ones, were still observed after the depuration period. These may reflect either the continuing chemical effect due to incomplete elimination or an onset of recovery processes in the mussel gills. Our study shows that exposure of adult mussels to sublethal TP concentration results in the bioconcentration of this biocide in the tissues and modulates the expression of several proteins that may intervene in important metabolic pathways. (C) 2016 Elsevier B.V. All rights reserved.

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