1. Short-range Fgf signalling patterns hindbrain progenitors to induce the neurogenesis-to-oligodendrogenesis switch.
- Author
-
Yeung TJ and Wilkinson DG
- Subjects
- Animals, Gene Expression Regulation, Developmental, Cell Differentiation, Oligodendrocyte Transcription Factor 2 metabolism, Oligodendrocyte Transcription Factor 2 genetics, Basic Helix-Loop-Helix Transcription Factors metabolism, Basic Helix-Loop-Helix Transcription Factors genetics, SOXE Transcription Factors metabolism, SOXE Transcription Factors genetics, Rhombencephalon metabolism, Rhombencephalon cytology, Neurogenesis physiology, Neurogenesis genetics, Zebrafish embryology, Zebrafish metabolism, Fibroblast Growth Factors metabolism, Fibroblast Growth Factors genetics, Signal Transduction, Zebrafish Proteins metabolism, Zebrafish Proteins genetics, Oligodendroglia metabolism, Oligodendroglia cytology
- Abstract
In the vertebrate nervous system, neurogenesis generally precedes gliogenesis. The mechanisms driving the switch in cell type production and generation of the correct proportion of cell types remain unclear. Here, we show that Fgf20 signalling patterns progenitors to induce the switch from neurogenesis to oligodendrogenesis in the zebrafish hindbrain. Fgf20 emanating from earlier-born neurons signals at a short range to downregulate proneural gene expression in the segment centre with high spatial precision along both anterior-posterior and dorsal-ventral axes. This signal induces oligodendrocytes in the segment centre by upregulating olig2 and sox10 expression in pre-patterned competent progenitors. We show that the magnitude of proneural gene downregulation and the quantity of oligodendrocyte precursor cells specified is dependent on the extent of Fgf20 signalling. Overexpression of fgf20a induces precocious specification and differentiation of oligodendrocytes among olig2+ progenitors, resulting in an increase in oligodendrocytes at the expense of neurogenesis. Thus, Fgf20 signalling defines the proportion of each cell type produced. Taken together, Fgf20 signalling from earlier-born neurons patterns hindbrain segments spatially and temporally to induce the neurogenesis-to-oligodendrogenesis switch., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2024. Published by The Company of Biologists Ltd.) more...
- Published
- 2024
- Full Text
- View/download PDF